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Papers by Suchada Chutimaworapan

Journal of Pharmaceutical Science and Technology, Japan, Sep 20, 2000

Journal of Pharmaceutical Science and Technology, Japan, Mar 20, 2000

Drug Development and Industrial Pharmacy, 2000

T he solid binary systems of aceclofenac (AC) with β-cyclodextrin (βCD) were prepared by co-grind... more T he solid binary systems of aceclofenac (AC) with β-cyclodextrin (βCD) were prepared by co-grinding, kneading, and co-evaporation, and with PEG 6000 were prepared by the melt-solvent method in 1:1 and 1:2 molar and weight ratios, respectively. The phase solubility study with βCD suggested B S type of curve with a possibility of 1:1 inclusion complex. The solid systems were characterized by in vitro release studies, DSC, and SEM. The results of solid state studies revealed that AC was completely dissolved in the carrier matrix in case of the melt-solvent method, which suggested the possible formation of solid solution with AC to be existed in an amorphous state. All the binary systems exhibited improved dissolution as compared to pure drug. However, the best dissolution enhancement was achieved with the binary system AC: PEG 6000 in 1:2 weight ratio using the melt-solvent method which was subjected to tablet preparation by direct compression. The tablets so compressed complied with in-house and compendial specifications. The in vitro dissolution test was carried out for the formulated tablets and three popular marketed brands of conventional AC tablets. None of the commercial brands showed complete drug release but the formulated tablets exhibited almost complete drug release within 50 min. The dissolution data were further characterized using model-independent parameters DP 30 , DE 50 , t 50% , similarity factor f 2 and difference factor f 1. The tablets formulated incorporating the AC: PEG 6000 (1:2) binary system displayed significantly improved dissolution profile as compared to existing immediate release commercial tablets.

The archives of practical pharmacy, Mar 20, 2001

Journal of Pharmaceutical Science and Technology, Japan, 2001

Journal of Pharmaceutical Science and Technology, Japan, 2000

Journal of Pharmaceutical Science and Technology, Japan, 2000

The purposes of this study were to develop chitosan microspheres containing doxycycline hyclate a... more The purposes of this study were to develop chitosan microspheres containing doxycycline hyclate and incorporate into glyceryl monooleate-based drug delivery system for the treatment of periodontal disease. The chitosan microspheres were prepared by using emulsification and ionotropic gelation method. The Box-Behnken experimental design was used as a tool for optimize the formulation. From the result obtained the fomulations comprising of doxycycline hyclate load (%), chitosan (%) and sodium tripolyphosphate (STPP) (%) as 75:3:15, 75:4:10 and 30:3:10 were selected. The microspheres could sustain release of doxycycline hyclate over a period of 24 hrs and followed square root of time kinetic which indicated that the rate of release was diffusion controlled. The accelerated stability study at 40, 50, 60 and 70 ℃ and the Arrhenius plot indicated that the predicted shelf-lives of microsphere formulations comprising of doxycycline hyclate load (%), chitosan (%) and STPP (%) of 75:3:15, 75:...

The in situ gelling suppositories containing Centella asiatica extracts spray-dried with chitosan... more The in situ gelling suppositories containing Centella asiatica extracts spray-dried with chitosan were formulated with poloxamer mixtures in this investigation. An experimental factorial design was built to investigate the effects of five parameters on percentage production yield and percentage moisture content of spray-dried products. These factors concerned both formulation parameters (%solid content, %additive concentration and polymer: extract ratio) and spray drying parameters (inlet temperature and feed rate). The operating condition was to maximize production yields while minimizing moisture contents. First screening experiments consisting of 25-1 fractional factorial design revealed the most significant factors to be inlet temperature and %solid content (P < 0.01). Then, the optimal operating condition was estimated by response surface methodology. Central rotational composite design showed a quadratic model for %yield and a linear model for %moisture content were adequat...

Antibacterial activity of mangosteen pericarp extract against cariogenic

Objective The purpose of this study was to examine the antibacterial activity of extract from man... more Objective The purpose of this study was to examine the antibacterial activity of extract from mangosteen pericarp against Streptococcus mutans, bacteria associated with dental plaque formation and caries development. Materials and methods Bacterial strains used in this study were S. mutans ATCC 25175 and KPSK2. Antibacterial activity was expressed as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The rates at which bacteria were killed were also determined by exposure to the extract at 2x and 4x MBC for varying time periods. Results The extract was effective against both strains of S. mutans. The MIC and MBC for the strain ATCC 25175 were both equal to 0.625 μg/ml. The respective values for the strain KPSK2 were 0.625 and 1.25 μg/ml. The MIC and MBC were comparable to those of chlorhexidine, an antiseptic commonly used in dental plaque control. Time-kill assays for the strain ATCC 25175 showed that treatment with the extract at 2x MBC caused a s...

Objective The aim of this study was to investigate the toxicity of mangosteen pericarp extract to... more Objective The aim of this study was to investigate the toxicity of mangosteen pericarp extract to human gingival fibroblasts in vitro. Materials and methods Human gingival fibroblasts were exposed to mangosteen extract at the concentrations of 0, 100, 200, 400, 800 or 1,600 µg/ml for 24 or 48 hours. The changes in cell viability were observed by inverted phase contrast microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results MTT assay showed that mangosteen extract at the concentrations of 200 µg/ml or less did not significantly affect cell viability. The extract at 400 µg/ml did not affect the number of viable cells at 24 hours, but significantly decreased cell survival at 48 hours. The extract at 800 µg/ml or higher resulted in a significant decrease in cell viability within 24 hours, and the number of viable cells was further declined after 48-hour exposure. The results from morphological analysis generally agreed with those from the MTT as...

การศกษาไนเฟดพนโซลดเพอรชนในกลมโพลเอทธลนไกลคอล (พอจ 4000 และพอจ 6000) กลมโพลอกซาเมอร (โพลอกซาเมอร 1... more การศกษาไนเฟดพนโซลดเพอรชนในกลมโพลเอทธลนไกลคอล (พอจ 4000 และพอจ 6000) กลมโพลอกซาเมอร (โพลอกซาเมอร 188, โพลอกซาเมอร 288 และโพลอกซาเมอร407) บตาไซโคลเดกซตรน และไฮดรอกซโพรพลบตาไซโคลเดกซทรน) โดยใชอตราสวนของตวยาตอตวพาเทากบ 1:1, 1:3, 1:5, และ 1:10 และเตรยมโดยวธการหลอมเหลว การใชตวทำละลายและการนวดผสมเปรยบเทยบกบของผสมทางกายภาพ พบวา อตราสวนของตวยาและตวพา 1:10 และเตรยมโดยวธหลอมเหลวและการใชตวทำลายของระบบสวนใหญจะใหคาอตราการละลายสงกวาระบบอน (p<0.05) อตราการละลายเพมขนสงสดในกลมโพลอกซาเมอร อตราการละลายทเพมขนสงเดนชดและเวลาทยาละลายได 80% ในเวลาเพยง 15 นาท สามารถไดจากการใชโพลอกซาเมอร 188 และโพลอกซาเมอร407 โดยวธการหลอมเหลวในอตราสวน 1:3, 1:5 และ1:10 อตราการละลายของพอจ 4000 และพอจ 6000 มคาสงใกลเคยงกนมาเมอเปรยบเทยบระหวางวธการเตรยมและอตราสวนเดยวกน สำหรบกลมบตาไซโคลเดกซทรนและไฮดรอกซโพรพลบตาไซโคลเดกซทรนใหคาคงทของอตราการละลายเพมขนเพยงเลกนอย การศกษาลกษณะทางเคมฟสกสพบวากลไกสำคญของการปลดปลอยเรวคอ การเกดรปอสณฐานของนเฟดพนในตวพาซงแสดงจากเอกซเรยดฟแฟรกชนและดฟเฟอเรนเชยลสแกนนงแคลอรเมตร การเพมการเปยกนำและคาการละลายมผลดตอกา...

Drug Development and Industrial Pharmacy, 2000

Solid dispersions of nifedipine (NP) with polyethylene glycols (PEG4000 and PEG6000), hydroxyprop... more Solid dispersions of nifedipine (NP) with polyethylene glycols (PEG4000 and PEG6000), hydroxypropyl-beta-cyclodextrin (HP beta CD), and poloxamer 407 (PXM 407) in four mixing ratios were prepared by melting, solvent, and kneading methods in order to improve the dissolution of NP. The enhancement of the dissolution rate and the time for 80% NP dissolution T80% depended on the mixing ratio and the preparation method. The highest dissolution rate and the T80% as short as 15 min were obtained from PXM 407 solid dispersion prepared by the melting method at the mixing ratio of 1:10. The X-ray diffraction (XRD) patterns of solid dispersions at higher proportions of carriers demonstrated consistent with the results from differential scanning calorimetric (DSC) thermograms that NP existed in the amorphous state. The wettability and solubility were markedly improved in the PXM 407 system. The presence of intermolecular hydrogen bonding between NP and PEGs and between HP beta CD and PXM 407 was shown by infrared (IR) spectroscopy.

Journal of Pharmaceutical Science and Technology, Japan, Sep 20, 2000

Journal of Pharmaceutical Science and Technology, Japan, Mar 20, 2000

Drug Development and Industrial Pharmacy, 2000

T he solid binary systems of aceclofenac (AC) with β-cyclodextrin (βCD) were prepared by co-grind... more T he solid binary systems of aceclofenac (AC) with β-cyclodextrin (βCD) were prepared by co-grinding, kneading, and co-evaporation, and with PEG 6000 were prepared by the melt-solvent method in 1:1 and 1:2 molar and weight ratios, respectively. The phase solubility study with βCD suggested B S type of curve with a possibility of 1:1 inclusion complex. The solid systems were characterized by in vitro release studies, DSC, and SEM. The results of solid state studies revealed that AC was completely dissolved in the carrier matrix in case of the melt-solvent method, which suggested the possible formation of solid solution with AC to be existed in an amorphous state. All the binary systems exhibited improved dissolution as compared to pure drug. However, the best dissolution enhancement was achieved with the binary system AC: PEG 6000 in 1:2 weight ratio using the melt-solvent method which was subjected to tablet preparation by direct compression. The tablets so compressed complied with in-house and compendial specifications. The in vitro dissolution test was carried out for the formulated tablets and three popular marketed brands of conventional AC tablets. None of the commercial brands showed complete drug release but the formulated tablets exhibited almost complete drug release within 50 min. The dissolution data were further characterized using model-independent parameters DP 30 , DE 50 , t 50% , similarity factor f 2 and difference factor f 1. The tablets formulated incorporating the AC: PEG 6000 (1:2) binary system displayed significantly improved dissolution profile as compared to existing immediate release commercial tablets.

The archives of practical pharmacy, Mar 20, 2001

Journal of Pharmaceutical Science and Technology, Japan, 2001

Journal of Pharmaceutical Science and Technology, Japan, 2000

Journal of Pharmaceutical Science and Technology, Japan, 2000

The purposes of this study were to develop chitosan microspheres containing doxycycline hyclate a... more The purposes of this study were to develop chitosan microspheres containing doxycycline hyclate and incorporate into glyceryl monooleate-based drug delivery system for the treatment of periodontal disease. The chitosan microspheres were prepared by using emulsification and ionotropic gelation method. The Box-Behnken experimental design was used as a tool for optimize the formulation. From the result obtained the fomulations comprising of doxycycline hyclate load (%), chitosan (%) and sodium tripolyphosphate (STPP) (%) as 75:3:15, 75:4:10 and 30:3:10 were selected. The microspheres could sustain release of doxycycline hyclate over a period of 24 hrs and followed square root of time kinetic which indicated that the rate of release was diffusion controlled. The accelerated stability study at 40, 50, 60 and 70 ℃ and the Arrhenius plot indicated that the predicted shelf-lives of microsphere formulations comprising of doxycycline hyclate load (%), chitosan (%) and STPP (%) of 75:3:15, 75:...

The in situ gelling suppositories containing Centella asiatica extracts spray-dried with chitosan... more The in situ gelling suppositories containing Centella asiatica extracts spray-dried with chitosan were formulated with poloxamer mixtures in this investigation. An experimental factorial design was built to investigate the effects of five parameters on percentage production yield and percentage moisture content of spray-dried products. These factors concerned both formulation parameters (%solid content, %additive concentration and polymer: extract ratio) and spray drying parameters (inlet temperature and feed rate). The operating condition was to maximize production yields while minimizing moisture contents. First screening experiments consisting of 25-1 fractional factorial design revealed the most significant factors to be inlet temperature and %solid content (P < 0.01). Then, the optimal operating condition was estimated by response surface methodology. Central rotational composite design showed a quadratic model for %yield and a linear model for %moisture content were adequat...

Antibacterial activity of mangosteen pericarp extract against cariogenic

Objective The purpose of this study was to examine the antibacterial activity of extract from man... more Objective The purpose of this study was to examine the antibacterial activity of extract from mangosteen pericarp against Streptococcus mutans, bacteria associated with dental plaque formation and caries development. Materials and methods Bacterial strains used in this study were S. mutans ATCC 25175 and KPSK2. Antibacterial activity was expressed as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The rates at which bacteria were killed were also determined by exposure to the extract at 2x and 4x MBC for varying time periods. Results The extract was effective against both strains of S. mutans. The MIC and MBC for the strain ATCC 25175 were both equal to 0.625 μg/ml. The respective values for the strain KPSK2 were 0.625 and 1.25 μg/ml. The MIC and MBC were comparable to those of chlorhexidine, an antiseptic commonly used in dental plaque control. Time-kill assays for the strain ATCC 25175 showed that treatment with the extract at 2x MBC caused a s...

Objective The aim of this study was to investigate the toxicity of mangosteen pericarp extract to... more Objective The aim of this study was to investigate the toxicity of mangosteen pericarp extract to human gingival fibroblasts in vitro. Materials and methods Human gingival fibroblasts were exposed to mangosteen extract at the concentrations of 0, 100, 200, 400, 800 or 1,600 µg/ml for 24 or 48 hours. The changes in cell viability were observed by inverted phase contrast microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results MTT assay showed that mangosteen extract at the concentrations of 200 µg/ml or less did not significantly affect cell viability. The extract at 400 µg/ml did not affect the number of viable cells at 24 hours, but significantly decreased cell survival at 48 hours. The extract at 800 µg/ml or higher resulted in a significant decrease in cell viability within 24 hours, and the number of viable cells was further declined after 48-hour exposure. The results from morphological analysis generally agreed with those from the MTT as...

การศกษาไนเฟดพนโซลดเพอรชนในกลมโพลเอทธลนไกลคอล (พอจ 4000 และพอจ 6000) กลมโพลอกซาเมอร (โพลอกซาเมอร 1... more การศกษาไนเฟดพนโซลดเพอรชนในกลมโพลเอทธลนไกลคอล (พอจ 4000 และพอจ 6000) กลมโพลอกซาเมอร (โพลอกซาเมอร 188, โพลอกซาเมอร 288 และโพลอกซาเมอร407) บตาไซโคลเดกซตรน และไฮดรอกซโพรพลบตาไซโคลเดกซทรน) โดยใชอตราสวนของตวยาตอตวพาเทากบ 1:1, 1:3, 1:5, และ 1:10 และเตรยมโดยวธการหลอมเหลว การใชตวทำละลายและการนวดผสมเปรยบเทยบกบของผสมทางกายภาพ พบวา อตราสวนของตวยาและตวพา 1:10 และเตรยมโดยวธหลอมเหลวและการใชตวทำลายของระบบสวนใหญจะใหคาอตราการละลายสงกวาระบบอน (p<0.05) อตราการละลายเพมขนสงสดในกลมโพลอกซาเมอร อตราการละลายทเพมขนสงเดนชดและเวลาทยาละลายได 80% ในเวลาเพยง 15 นาท สามารถไดจากการใชโพลอกซาเมอร 188 และโพลอกซาเมอร407 โดยวธการหลอมเหลวในอตราสวน 1:3, 1:5 และ1:10 อตราการละลายของพอจ 4000 และพอจ 6000 มคาสงใกลเคยงกนมาเมอเปรยบเทยบระหวางวธการเตรยมและอตราสวนเดยวกน สำหรบกลมบตาไซโคลเดกซทรนและไฮดรอกซโพรพลบตาไซโคลเดกซทรนใหคาคงทของอตราการละลายเพมขนเพยงเลกนอย การศกษาลกษณะทางเคมฟสกสพบวากลไกสำคญของการปลดปลอยเรวคอ การเกดรปอสณฐานของนเฟดพนในตวพาซงแสดงจากเอกซเรยดฟแฟรกชนและดฟเฟอเรนเชยลสแกนนงแคลอรเมตร การเพมการเปยกนำและคาการละลายมผลดตอกา...

Drug Development and Industrial Pharmacy, 2000

Solid dispersions of nifedipine (NP) with polyethylene glycols (PEG4000 and PEG6000), hydroxyprop... more Solid dispersions of nifedipine (NP) with polyethylene glycols (PEG4000 and PEG6000), hydroxypropyl-beta-cyclodextrin (HP beta CD), and poloxamer 407 (PXM 407) in four mixing ratios were prepared by melting, solvent, and kneading methods in order to improve the dissolution of NP. The enhancement of the dissolution rate and the time for 80% NP dissolution T80% depended on the mixing ratio and the preparation method. The highest dissolution rate and the T80% as short as 15 min were obtained from PXM 407 solid dispersion prepared by the melting method at the mixing ratio of 1:10. The X-ray diffraction (XRD) patterns of solid dispersions at higher proportions of carriers demonstrated consistent with the results from differential scanning calorimetric (DSC) thermograms that NP existed in the amorphous state. The wettability and solubility were markedly improved in the PXM 407 system. The presence of intermolecular hydrogen bonding between NP and PEGs and between HP beta CD and PXM 407 was shown by infrared (IR) spectroscopy.