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Papers by Shehu Danlami
Acta Biologica Marisiensis, 2021
Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. ... more Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. The Pf HRP2 based RDT kit is the most widely used RDT for malaria diagnosis in Nigeria but is affected by the deletion of HRP2 gene in Plasmodium falciparum parasites. Therefore, identifying the prevalence and distribution of Plasmodium falciparum parasites with deleted Pf HRP2 is important for malaria control. Pf HRP2 gene deletion was assessed in this study by first carrying out Giemsa stained thick blood film microscopy and Pf HRP2 RDT strip test. The samples were further analyzed for molecular examination by PCR assay for multiple single–copy genes (Pf Cox3, Pf HRP2, Pf HRP3 and Pf Beta tubulin). This study found the existence of eight (8) Plasmodium falciparum isolates lacking the HRP2 gene in the samples analyzed, this necessitates the need to develop a unique RDT Kit targeting other housekeeping genes unique for Plasmodium falciparum with far greater sensitivity than the current o...
Acta Biologica Marisiensis, 2021
Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. ... more Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. The Pf HRP2 based RDT kit is the most widely used RDT for malaria diagnosis in Nigeria but is affected by the deletion of HRP2 gene in Plasmodium falciparum parasites. Therefore, identifying the prevalence and distribution of Plasmodium falciparum parasites with deleted Pf HRP2 is important for malaria control. Pf HRP2 gene deletion was assessed in this study by first carrying out Giemsa stained thick blood film microscopy and Pf HRP2 RDT strip test. The samples were further analyzed for molecular examination by PCR assay for multiple single–copy genes (Pf Cox3, Pf HRP2, Pf HRP3 and Pf Beta tubulin). This study found the existence of eight (8) Plasmodium falciparum isolates lacking the HRP2 gene in the samples analyzed, this necessitates the need to develop a unique RDT Kit targeting other housekeeping genes unique for Plasmodium falciparum with far greater sensitivity than the current o...
Acta Biologica Marisiensis, 2021
Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. ... more Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. The Pf HRP2 based RDT kit is the most widely used RDT for malaria diagnosis in Nigeria but is affected by the deletion of HRP2 gene in Plasmodium falciparum parasites. Therefore, identifying the prevalence and distribution of Plasmodium falciparum parasites with deleted Pf HRP2 is important for malaria control. Pf HRP2 gene deletion was assessed in this study by first carrying out Giemsa stained thick blood film microscopy and Pf HRP2 RDT strip test. The samples were further analyzed for molecular examination by PCR assay for multiple single–copy genes (Pf Cox3, Pf HRP2, Pf HRP3 and Pf Beta tubulin). This study found the existence of eight (8) Plasmodium falciparum isolates lacking the HRP2 gene in the samples analyzed, this necessitates the need to develop a unique RDT Kit targeting other housekeeping genes unique for Plasmodium falciparum with far greater sensitivity than the current o...
Women responses to pain during child birth might be different. Some women cried, some would shout... more Women responses to pain during child birth might be different. Some women cried, some would shout, while some were observed to be quite, calm and responding to midwives’ instructions. The aim of this study was to examine perception of pain and factors influencing pain response among parturients. Study would provide information regarding the needs of women for providing efficient care and support in labour. The study was a descriptive cross-sectional in nature in which 51 questionnaires were distributed to the women, 46 completed were collected, data analyzed and presented in descriptive form. Findings suggest that majority of women experienced mild to moderate pain in labour. Fear of labour and uncomfortable positions were the major factors aggravating labour pain. In order to reduce labour pain most women preferred reassurance, good attitude of midwives and good environmental condition. Therefore, midwives should provide support, encouragement as well as patient-centered care to wo...
Acta Biologica Marisiensis, 2021
Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. ... more Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. The Pf HRP2 based RDT kit is the most widely used RDT for malaria diagnosis in Nigeria but is affected by the deletion of HRP2 gene in Plasmodium falciparum parasites. Therefore, identifying the prevalence and distribution of Plasmodium falciparum parasites with deleted Pf HRP2 is important for malaria control. Pf HRP2 gene deletion was assessed in this study by first carrying out Giemsa stained thick blood film microscopy and Pf HRP2 RDT strip test. The samples were further analyzed for molecular examination by PCR assay for multiple single–copy genes (Pf Cox3, Pf HRP2, Pf HRP3 and Pf Beta tubulin). This study found the existence of eight (8) Plasmodium falciparum isolates lacking the HRP2 gene in the samples analyzed, this necessitates the need to develop a unique RDT Kit targeting other housekeeping genes unique for Plasmodium falciparum with far greater sensitivity than the current o...
Acta Biologica Marisiensis, 2021
Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. ... more Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. The Pf HRP2 based RDT kit is the most widely used RDT for malaria diagnosis in Nigeria but is affected by the deletion of HRP2 gene in Plasmodium falciparum parasites. Therefore, identifying the prevalence and distribution of Plasmodium falciparum parasites with deleted Pf HRP2 is important for malaria control. Pf HRP2 gene deletion was assessed in this study by first carrying out Giemsa stained thick blood film microscopy and Pf HRP2 RDT strip test. The samples were further analyzed for molecular examination by PCR assay for multiple single–copy genes (Pf Cox3, Pf HRP2, Pf HRP3 and Pf Beta tubulin). This study found the existence of eight (8) Plasmodium falciparum isolates lacking the HRP2 gene in the samples analyzed, this necessitates the need to develop a unique RDT Kit targeting other housekeeping genes unique for Plasmodium falciparum with far greater sensitivity than the current o...
Acta Biologica Marisiensis, 2021
Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. ... more Malaria Rapid Diagnostic Tests (RDTs) plays an important role in malaria management and control. The Pf HRP2 based RDT kit is the most widely used RDT for malaria diagnosis in Nigeria but is affected by the deletion of HRP2 gene in Plasmodium falciparum parasites. Therefore, identifying the prevalence and distribution of Plasmodium falciparum parasites with deleted Pf HRP2 is important for malaria control. Pf HRP2 gene deletion was assessed in this study by first carrying out Giemsa stained thick blood film microscopy and Pf HRP2 RDT strip test. The samples were further analyzed for molecular examination by PCR assay for multiple single–copy genes (Pf Cox3, Pf HRP2, Pf HRP3 and Pf Beta tubulin). This study found the existence of eight (8) Plasmodium falciparum isolates lacking the HRP2 gene in the samples analyzed, this necessitates the need to develop a unique RDT Kit targeting other housekeeping genes unique for Plasmodium falciparum with far greater sensitivity than the current o...
Women responses to pain during child birth might be different. Some women cried, some would shout... more Women responses to pain during child birth might be different. Some women cried, some would shout, while some were observed to be quite, calm and responding to midwives’ instructions. The aim of this study was to examine perception of pain and factors influencing pain response among parturients. Study would provide information regarding the needs of women for providing efficient care and support in labour. The study was a descriptive cross-sectional in nature in which 51 questionnaires were distributed to the women, 46 completed were collected, data analyzed and presented in descriptive form. Findings suggest that majority of women experienced mild to moderate pain in labour. Fear of labour and uncomfortable positions were the major factors aggravating labour pain. In order to reduce labour pain most women preferred reassurance, good attitude of midwives and good environmental condition. Therefore, midwives should provide support, encouragement as well as patient-centered care to wo...