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Papers by S.KRISHNA SUNDARI

Indo global journal of pharmaceutical sciences, 2017

Indo Global Journal of Pharmaceutical Sciences, 2017

International journal of nanobiotechnology, Oct 31, 2017

Indo Global Journal of Pharmaceutical Sciences, 2017

Indo Global Journal of Pharmaceutical Sciences, 2017

Journal of Bioprocessing & Biotechniques, 2015

Modern sustainable agricultural practices prefer to use biological agents for plant growth promot... more Modern sustainable agricultural practices prefer to use biological agents for plant growth promotion, biocontrol and bioremediation as these are cost effective and eco-friendly. Our present study aims to investigate the effects of direct inoculation of selected consortia on plants to study its effects on supporting plant growth in the presence of root pathogen Sclerotium rolfsii and organophosphate pesticides Malathion (ML) and Methyl Parathion (MP). Candidate Plant Growth Promoting Microbial (PGPM) isolates chosen for the study are two bacterial isolates (PGPM2 a diazotrophic bacterium, PGPM9 a fluorescent Pseudomonad) and one fungal species (T103 a biocontrol fungus), originating from native agricultural fields of western U.P., India. Host plants inoculated with individual species showed a distributed growth enhancement pattern i.e., while isolate T103 improved root biomass, isolate PGPM9 enriched photosynthetic pigment content and isolate PGPM2 expanded root and shoot lengths. It appeared as though individual isolates showed a preference to enhance certain parameters over the other rather than exhibiting a uniform increment in all growth parameters. This preference to specific growth parameter over the other waned off in consortium studies where Sorghum bicolor inoculated with the consortium registered almost 2-fold increase in all parameters viz., root length, shoot length and overall biomass (root, shoot and total biomass) along with 23% rise in total chlorophyll content as compared to un-inoculated control. Selected consortia combination was able to provide better growth promotion in presence of pathogen Sclerotium rolfsii registering 58% increase in total biomass content while individual inoculation of biocontrol fungus T103 showed only 36% improvement. Selected consortia were also effective in plant growth promotion in presence of organophosphate pesticides ML and MP. More than two fold amplification was registered in all roots and shoot growth parameters studied when consortia was provided with ML and 28% increase was recorded when MP treatment was countered with consortia inoculation. All these results affirms our hypothesis that synergistic action of carefully selected PGPMs can escalate the benefits of plant growth promotion even in presence of pathogen and pesticide, hence this consortia may be a valuable option for sustained plant growth in modern agriculture systems.

Ecology, Environment and Conservation

Organic and inorganic pollutants have deleterious effect in the environment, crops productivity, ... more Organic and inorganic pollutants have deleterious effect in the environment, crops productivity, microbiomeand soil fertility. The persistence uses of toxic compound like pesticides not only affect soil health but alsoagitated ecosystem. Use of Plant Growth Promoting rhizobacteria can play an important role towardsachieving the objectives of sustainable ecosystem. Pseudomonas group of bacteria are known for theirremediation properties but are lesser explored for their growth promotion attributes and to alleviate otherabiotic and biotic stress in the agriculture system. The paper presents multifarious potential of Pseudomonasisolates obtained from agriculture fields in the vicinity of Ghaziabad, U.P (PGPM2, 3, 4, 5). Biochemicaltests for identification suggested that these isolates belong to Pseudomonads group of organisms. Further,molecular characterization through 16S RNA gene sequence analysis confirmed their identity as PGPM2-Pseudomonas species, PGPM3- Pseudomonas plecoglosida, ...

Indo global journal of pharmaceutical sciences, 2017

Indo global journal of pharmaceutical sciences, 2017

Indo global journal of pharmaceutical sciences, 2017

Indo global journal of pharmaceutical sciences, 2014

Farmers across the world face the challenge of compromised agriculture productivity due to abioti... more Farmers across the world face the challenge of compromised agriculture productivity due to abiotic stress. Residual pesticides accumulating in the soil rhizospere are often found to cause a chemical imbalance in the soil microclimate and cause chemical stress and toxicity. Plant growth promoting rhizobacteria (PGPR) are popularly studied for

Indo Global Journal of Pharmaceutical Sciences, 2017

Indo Global Journal of Pharmaceutical Sciences, 2014

The cellular system may have an imbalance between the oxidants and anti-oxidants, simply caused e... more The cellular system may have an imbalance between the oxidants and anti-oxidants, simply caused eitherby decreased antioxidant defense system or an increased production of Reactive Oxygen Species (ROS). The ROS over production leads to conditions of oxidative stress. The present review discusses how ROS play a major role in triggering / accelerating processes related to early ageing.ROS were found to be capable of damaging mitochondrial DNA,

In India more than 40% of solid waste generated annually is from organic and agricultural sources... more In India more than 40% of solid waste generated annually is from organic and agricultural sources. There is a growing concern for these accumulating wastes as they are either being dumped in landfills, burnt, or left to rot in the open, leading to severe environmental pollution. A sustainable solution would be to utilize these solid wastes as an ‘economical’ alternative to costly raw materials and produce industrially important products of practical utility. Tannase and the associated byproduct gallic acid have many potential applications in food, pharmaceutical and chemical industries. Many plant species have naturally occurring tannins in their biomass. The present study focuses on utilization of these solid food and agricultural residues (FAR) as a source of tannic acid for producing tannase and gallic acid through microbial fermentation. Native micro-organisms were isolated from FAR and soil from FAR dump sites in Delhi NCR region. Screening and selection of tannase producing is...

Microorganisms for Sustainability, 2019

Increasing population has led to increased demand of food supply which marks greater importance t... more Increasing population has led to increased demand of food supply which marks greater importance to the agriculture sector worldwide. Chemical pesticides applied with an intention to maximize agriculture productivity and crop yield are leaving high residues in the agricultural soils, influencing the soil health adversely. Plant growth-promoting microbes (PGPMs) are largely known to be applied for their role in enhancing plant growth, thereby becoming a favourable choice for developing the new age bioinoculants. Presently, many researchers are concentrating their efforts to explore the potential of PGPMs to fight this problem of chemical residue accumulation in soils. Employing PGPM for bioremediation process would provide dual benefits to agricultural fields.

i-manager’s Journal on Management, 2015

Current Science, 2017

In this study a stepwise, statistically verifiable scientific protocol-'six-step-strategy'-to dev... more In this study a stepwise, statistically verifiable scientific protocol-'six-step-strategy'-to develop a consortium is presented. Additionally, it introduces a novel in vivo plant bioassay-'tube-in-tube' method-that gives faster (< three weeks) and reproducible results for selecting the most desirable consortia combinations. The study employs eight plant growth promoting microbes (PGPMs) with pre-established growth supporting abilities and no mutual antagonism. Following a two-factorial design, 15 consortia combinations (CCs) were developed from these PGPMs. Applying the principles of the 'six-step-strategy', combinations CC11, CC13 and CC4 showing significant increments (>100%) in root length and dry weight were recognized as the best performing consortia. The method thus shortlists the best and manageable number of consortia for further field trials.

MethodsX, 2019

Bioinoculant studies focusing on plant-microbe association hold immense importance for research i... more Bioinoculant studies focusing on plant-microbe association hold immense importance for research in field of agriculture and plant science. Such plant-microbe associations are amongst the most complex and beneficial partnerships in nature. Research focused to explore favourable host-microbe relationship requires a contamination free, non-interceptive and easily manageable system where these interactions can be studied in real-time. Also a provision for efficient sample recovery to support a variety of analyses would be a definite advantage. The manuscript proposes a new multi-potential plant bioassay abbreviated "ASURE" inspired by hydroponics. ASURE is a robust system, providing a workable solution to challenges faced during in-vitro microcosm studies. Significance of this system is its plant growth supporting design, facilitating comparative assessment of PGPM treatment benefits to host plant while providing a closer real-time view of plant microbe association. ASURE serves as a testing tool to predetermine the efficiency of various plant friendly microbes to develop them as future bioinoculants. ASURE accommodates 16 individual samples in a 12 Â 12 cm space, ensures homogenous micro environment and facilitates continuous and undisturbed (real-time) monitoring of root and shoot growth. ASURE enables efficient sample recovery with zero loss during harvesting and no interception from substrate.

World Journal of Microbiology and Biotechnology, 2016

Species of Trichoderma are widely recognized for their biocontrol abilities, but seldom studied c... more Species of Trichoderma are widely recognized for their biocontrol abilities, but seldom studied collectively, for their plant growth promotion, abiotic stress tolerance and bioremediation properties. Our study is a concentrated effort to establish the potential of native isolate Trichoderma harzianum KSNM (T103) to tolerate biotic (root pathogens) and abiotic stresses [high salt (100-1000 mM); heavy metal (chromium, nickel and zinc: 1-10 mM); pesticides: malathion (100-600 ppm), carbofuran (100-600 ppb)], along with its ability to support plant growth. In vitro growth promotion assays with T103 treated Vigna radiata, Vigna mungo and Hordeum vulgare confirmed 'non-species specific' growth promotion effects of T103. At lower metal concentration, T103 treatment was found to completely negate the impact of metal stress [60 % increase in radicle length (RL) with no significant decrease in %germination (%G)]. Even at 10 mM metal, T103 inoculation gave 80 % increase in %G and [50 % increase in RL. In vitro experiments confirmed high metal reduction capacity (47 %-Cr, 35 %-Ni and 42 %-Zn) of T103 at concentrations as high as 4 mM. At maximum residual concentrations of malathion (440 ppm) and carbofuran (100 ppb) reported in agricultural soils, T103 maintained 80 and 100 % survivability, respectively. T103 treatment has improved %G and RL in all three hosts challenged with pesticide. Isolate T103 was found to effectively suppress growth of three major root pathogens: Macrophomina phaseolina (65.83 %) followed by Sclerotium rolfsii (19.33 %) and Fusarium oxysporum (19.18 %). In the light of these observations, native T. harzianum (T103) seems to be a competent biocontrol agent for tropical agricultural soils contaminated with residual pesticides and heavy metals.

Indo global journal of pharmaceutical sciences, 2017

Indo Global Journal of Pharmaceutical Sciences, 2017

International journal of nanobiotechnology, Oct 31, 2017

Indo Global Journal of Pharmaceutical Sciences, 2017

Indo Global Journal of Pharmaceutical Sciences, 2017

Journal of Bioprocessing & Biotechniques, 2015

Modern sustainable agricultural practices prefer to use biological agents for plant growth promot... more Modern sustainable agricultural practices prefer to use biological agents for plant growth promotion, biocontrol and bioremediation as these are cost effective and eco-friendly. Our present study aims to investigate the effects of direct inoculation of selected consortia on plants to study its effects on supporting plant growth in the presence of root pathogen Sclerotium rolfsii and organophosphate pesticides Malathion (ML) and Methyl Parathion (MP). Candidate Plant Growth Promoting Microbial (PGPM) isolates chosen for the study are two bacterial isolates (PGPM2 a diazotrophic bacterium, PGPM9 a fluorescent Pseudomonad) and one fungal species (T103 a biocontrol fungus), originating from native agricultural fields of western U.P., India. Host plants inoculated with individual species showed a distributed growth enhancement pattern i.e., while isolate T103 improved root biomass, isolate PGPM9 enriched photosynthetic pigment content and isolate PGPM2 expanded root and shoot lengths. It appeared as though individual isolates showed a preference to enhance certain parameters over the other rather than exhibiting a uniform increment in all growth parameters. This preference to specific growth parameter over the other waned off in consortium studies where Sorghum bicolor inoculated with the consortium registered almost 2-fold increase in all parameters viz., root length, shoot length and overall biomass (root, shoot and total biomass) along with 23% rise in total chlorophyll content as compared to un-inoculated control. Selected consortia combination was able to provide better growth promotion in presence of pathogen Sclerotium rolfsii registering 58% increase in total biomass content while individual inoculation of biocontrol fungus T103 showed only 36% improvement. Selected consortia were also effective in plant growth promotion in presence of organophosphate pesticides ML and MP. More than two fold amplification was registered in all roots and shoot growth parameters studied when consortia was provided with ML and 28% increase was recorded when MP treatment was countered with consortia inoculation. All these results affirms our hypothesis that synergistic action of carefully selected PGPMs can escalate the benefits of plant growth promotion even in presence of pathogen and pesticide, hence this consortia may be a valuable option for sustained plant growth in modern agriculture systems.

Ecology, Environment and Conservation

Organic and inorganic pollutants have deleterious effect in the environment, crops productivity, ... more Organic and inorganic pollutants have deleterious effect in the environment, crops productivity, microbiomeand soil fertility. The persistence uses of toxic compound like pesticides not only affect soil health but alsoagitated ecosystem. Use of Plant Growth Promoting rhizobacteria can play an important role towardsachieving the objectives of sustainable ecosystem. Pseudomonas group of bacteria are known for theirremediation properties but are lesser explored for their growth promotion attributes and to alleviate otherabiotic and biotic stress in the agriculture system. The paper presents multifarious potential of Pseudomonasisolates obtained from agriculture fields in the vicinity of Ghaziabad, U.P (PGPM2, 3, 4, 5). Biochemicaltests for identification suggested that these isolates belong to Pseudomonads group of organisms. Further,molecular characterization through 16S RNA gene sequence analysis confirmed their identity as PGPM2-Pseudomonas species, PGPM3- Pseudomonas plecoglosida, ...

Indo global journal of pharmaceutical sciences, 2017

Indo global journal of pharmaceutical sciences, 2017

Indo global journal of pharmaceutical sciences, 2017

Indo global journal of pharmaceutical sciences, 2014

Farmers across the world face the challenge of compromised agriculture productivity due to abioti... more Farmers across the world face the challenge of compromised agriculture productivity due to abiotic stress. Residual pesticides accumulating in the soil rhizospere are often found to cause a chemical imbalance in the soil microclimate and cause chemical stress and toxicity. Plant growth promoting rhizobacteria (PGPR) are popularly studied for

Indo Global Journal of Pharmaceutical Sciences, 2017

Indo Global Journal of Pharmaceutical Sciences, 2014

The cellular system may have an imbalance between the oxidants and anti-oxidants, simply caused e... more The cellular system may have an imbalance between the oxidants and anti-oxidants, simply caused eitherby decreased antioxidant defense system or an increased production of Reactive Oxygen Species (ROS). The ROS over production leads to conditions of oxidative stress. The present review discusses how ROS play a major role in triggering / accelerating processes related to early ageing.ROS were found to be capable of damaging mitochondrial DNA,

In India more than 40% of solid waste generated annually is from organic and agricultural sources... more In India more than 40% of solid waste generated annually is from organic and agricultural sources. There is a growing concern for these accumulating wastes as they are either being dumped in landfills, burnt, or left to rot in the open, leading to severe environmental pollution. A sustainable solution would be to utilize these solid wastes as an ‘economical’ alternative to costly raw materials and produce industrially important products of practical utility. Tannase and the associated byproduct gallic acid have many potential applications in food, pharmaceutical and chemical industries. Many plant species have naturally occurring tannins in their biomass. The present study focuses on utilization of these solid food and agricultural residues (FAR) as a source of tannic acid for producing tannase and gallic acid through microbial fermentation. Native micro-organisms were isolated from FAR and soil from FAR dump sites in Delhi NCR region. Screening and selection of tannase producing is...

Microorganisms for Sustainability, 2019

Increasing population has led to increased demand of food supply which marks greater importance t... more Increasing population has led to increased demand of food supply which marks greater importance to the agriculture sector worldwide. Chemical pesticides applied with an intention to maximize agriculture productivity and crop yield are leaving high residues in the agricultural soils, influencing the soil health adversely. Plant growth-promoting microbes (PGPMs) are largely known to be applied for their role in enhancing plant growth, thereby becoming a favourable choice for developing the new age bioinoculants. Presently, many researchers are concentrating their efforts to explore the potential of PGPMs to fight this problem of chemical residue accumulation in soils. Employing PGPM for bioremediation process would provide dual benefits to agricultural fields.

i-manager’s Journal on Management, 2015

Current Science, 2017

In this study a stepwise, statistically verifiable scientific protocol-'six-step-strategy'-to dev... more In this study a stepwise, statistically verifiable scientific protocol-'six-step-strategy'-to develop a consortium is presented. Additionally, it introduces a novel in vivo plant bioassay-'tube-in-tube' method-that gives faster (< three weeks) and reproducible results for selecting the most desirable consortia combinations. The study employs eight plant growth promoting microbes (PGPMs) with pre-established growth supporting abilities and no mutual antagonism. Following a two-factorial design, 15 consortia combinations (CCs) were developed from these PGPMs. Applying the principles of the 'six-step-strategy', combinations CC11, CC13 and CC4 showing significant increments (>100%) in root length and dry weight were recognized as the best performing consortia. The method thus shortlists the best and manageable number of consortia for further field trials.

MethodsX, 2019

Bioinoculant studies focusing on plant-microbe association hold immense importance for research i... more Bioinoculant studies focusing on plant-microbe association hold immense importance for research in field of agriculture and plant science. Such plant-microbe associations are amongst the most complex and beneficial partnerships in nature. Research focused to explore favourable host-microbe relationship requires a contamination free, non-interceptive and easily manageable system where these interactions can be studied in real-time. Also a provision for efficient sample recovery to support a variety of analyses would be a definite advantage. The manuscript proposes a new multi-potential plant bioassay abbreviated "ASURE" inspired by hydroponics. ASURE is a robust system, providing a workable solution to challenges faced during in-vitro microcosm studies. Significance of this system is its plant growth supporting design, facilitating comparative assessment of PGPM treatment benefits to host plant while providing a closer real-time view of plant microbe association. ASURE serves as a testing tool to predetermine the efficiency of various plant friendly microbes to develop them as future bioinoculants. ASURE accommodates 16 individual samples in a 12 Â 12 cm space, ensures homogenous micro environment and facilitates continuous and undisturbed (real-time) monitoring of root and shoot growth. ASURE enables efficient sample recovery with zero loss during harvesting and no interception from substrate.

World Journal of Microbiology and Biotechnology, 2016

Species of Trichoderma are widely recognized for their biocontrol abilities, but seldom studied c... more Species of Trichoderma are widely recognized for their biocontrol abilities, but seldom studied collectively, for their plant growth promotion, abiotic stress tolerance and bioremediation properties. Our study is a concentrated effort to establish the potential of native isolate Trichoderma harzianum KSNM (T103) to tolerate biotic (root pathogens) and abiotic stresses [high salt (100-1000 mM); heavy metal (chromium, nickel and zinc: 1-10 mM); pesticides: malathion (100-600 ppm), carbofuran (100-600 ppb)], along with its ability to support plant growth. In vitro growth promotion assays with T103 treated Vigna radiata, Vigna mungo and Hordeum vulgare confirmed 'non-species specific' growth promotion effects of T103. At lower metal concentration, T103 treatment was found to completely negate the impact of metal stress [60 % increase in radicle length (RL) with no significant decrease in %germination (%G)]. Even at 10 mM metal, T103 inoculation gave 80 % increase in %G and [50 % increase in RL. In vitro experiments confirmed high metal reduction capacity (47 %-Cr, 35 %-Ni and 42 %-Zn) of T103 at concentrations as high as 4 mM. At maximum residual concentrations of malathion (440 ppm) and carbofuran (100 ppb) reported in agricultural soils, T103 maintained 80 and 100 % survivability, respectively. T103 treatment has improved %G and RL in all three hosts challenged with pesticide. Isolate T103 was found to effectively suppress growth of three major root pathogens: Macrophomina phaseolina (65.83 %) followed by Sclerotium rolfsii (19.33 %) and Fusarium oxysporum (19.18 %). In the light of these observations, native T. harzianum (T103) seems to be a competent biocontrol agent for tropical agricultural soils contaminated with residual pesticides and heavy metals.