Serap KURUCA - Academia.edu (original) (raw)

Papers by Serap KURUCA

Background: The present study was designed to evaluate the mechanism of cytotoxic effects of AE r... more Background: The present study was designed to evaluate the mechanism of cytotoxic effects of AE relative to Aloe vera gel extract (AVG) on chronic myelogenous leukemia K562 and Burkitt’s lymphoma P3HR-1 cell lines. Methods: Cytotoxicity tests were assessed by tetrazolium bromide (MTT) assay. Annexin V-FITC/PI labelling was used for apoptosis/necrosis evaluation and western blotting for apoptotic molecules measurement. Results: It was shown that, AE has cytotoxic activity againts K562 and P3HR-1 cells with an IC50 value of 60.9 µM and 28 µM, respectively. AVG was found cytotoxic on K562 cells with an IC50 value of 243.2 µg/ml and ineffective on P3HR-1 cells. The ratio of apoptotic cells (46.7%) was high in only K562 cells after AVG treatment. The percentage of apoptotic cells (K562=34.1, P3HR-1=38.8) was higher than necrotic cells (K562=11.9, P3HR-1=16.6) after AE treatment. The main apoptosis pathway in both cell lines was found to be through caspase-3 and caspase-9 activations afte...

Istanbul Journal of Pharmacy, 2018

Known for centuries as a "wonder" plant, Aloe vera (L.) Burm. f. has many biological and pharmaco... more Known for centuries as a "wonder" plant, Aloe vera (L.) Burm. f. has many biological and pharmacological activities. These effects are due to the variety of the chemical compounds including anthraquinones, glycoproteins, polysaccharides, vitamins and enzymes contained (Choi and Chung 2003; Du Plessis and Hamman 2014; Akev et al. 2015; Shrestha et al. 2015). Many of the medicinal effects have also been attributed to the immunomodulatory properties of the inner gel (Im et al. 2010), but it is also believed that synergistic action of the compounds contained in the whole leaf extracts is responsible for the multiple and diverse beneficial properties of the plant (Eshun and He 2004). The mucilageneous gel part of A. vera is used commercially as a softener in various cosmetic preparations, soaps and shampoos due to its glycoprotein content. The first known effects of A. vera after its cathartic activity, is the wound and burn healing effect of the leaves gel portion, widely supported in scientific literature (Capasso et al. 1998; Chithra et al. 1998; Heggers et al. 1995). Because of this effect, the gel is also added to many preparations used for skin treatment. Antitumor and cytotoxic potential of A. vera extracts continues to be the interest of scientific research from 1980's (Winters et al. 1981; Tsuda et al. 1993; Corsi et al. 1998) until recent years (Naveena Bharath and Selvasubramanian 2011; Du Plessis and Hamman 2014). In a research undertaken in our laboratory, A. vera leaf skin aqueous extract was proved to be effective as prophylactic against Ehrlich ascites tumours in vivo (Akev et al. 2007). The Food and Drug Administration of the USA has approved the developmental study of A. vera for the treatment of cancer and AIDS (Nandal and Bhardwaj 2012). Antitumor effect of aloe emodin (AE), the major anthraquinone derivative of A. vera, was reported in recent years (Cárdenas et al. 2006; Lee et al. 2006; Lin et al. 2006) and attention has been given recently to the possibility of utilizing AE as a chemothera

Macromolecular Materials and Engineering, 2018

The version in the Kent Academic Repository may differ from the final published version. Users ar... more The version in the Kent Academic Repository may differ from the final published version. Users are advised to check http://kar.kent.ac.uk for the status of the paper. Users should always cite the published version of record.

Asian Pacific Journal of Cancer Prevention, 2020

The multiple biological effects of the "wonder plant" Aloe vera (L.) Burm. f. (Xanthorraceae, for... more The multiple biological effects of the "wonder plant" Aloe vera (L.) Burm. f. (Xanthorraceae, formerly Aloaceae and Liliaceae) were proved and reviewed by scientific research (Akev et al., 2015). Several in vivo studies were conducted from 1980's to date with A. vera leaf extracts regarding their antitumour effects (Winters et al., 1981; Gribel and Pashinskiĭ, 1986; Akev et al., 2007a). Aloe emodin (AE) is an anthraquinone glycoside purified from A. vera. In addition to its well established laxative effect, AE have been reported to exhibit antiviral, antimicrobial, hepatoprotective and anticancer properties. Considerable attention has been given recently to the possibility of utilizing AE as a chemotherapeutic drug for the treatment of various types of cancers (Yordanova and Koprinarova, 2014). Lectins are proteins or glycoproteins that bind to specific sugar residues on cell surfaces (Sharon and Lis, 1972). Lectins play multiple roles in inter-and intra-cellular

ChemistrySelect, 2019

Bone tissue engineering studies have brought three-dimensional scaffolds into focus that can prov... more Bone tissue engineering studies have brought three-dimensional scaffolds into focus that can provide tissue regeneration with designed porosity and strengthened structure. Current research has concentrated on the fabrication of natural and synthetic polymer-based complex structures that closely mimic biological tissues due to their superior biocompatibility and biodegradabilities. Gelatine/Sodium Alginate hydrogels reinforced with different concentrations of β-Tricalcium Phosphate (TCP) (10, 13, and 15 wt.%) were studied to form 3D bone tissue. Physical, mechanical, chemical, morphological properties and biodegradability of the constructs were investigated. Furthermore, in vitro biological assay with human osteosarcoma cell line (SAOS-2) was performed to determine the biocompatibility of the constructs. It is found that cell viability rates for all constructs were increased and maximum cell viability rate was attained for 20%Gelatine/2%Alginate/10%TCP (wt.). The present work demonstrates that 3D printed Gelatine/ Alginate/TCP constructs with porous structures are potential candidates for bone tissue engineering applications.

European Polymer Journal, 2019

The use of natural compounds such as biocompatible and non-toxic plant extracts, without undesire... more The use of natural compounds such as biocompatible and non-toxic plant extracts, without undesired side effects, in tissue engineering applications, is highly preferred compared to chemical drugs. In this study, the characterization and performance of electrospun Achillea lycaonica-loaded (0.125, 0.250 and 0.500, wt%) poly (lactic acid) (PLA) (8%, w/v) nanofibrous mats for skin tissue engineering were investigated. SEM, FTIR, DSC, and tensile strength test of the electrospun nanofibers have been investigated. Drug releasing test and cell culture study were also carried out. Achillea lycaonica-loaded nanofibrous mats in 0.250 (wt%) and 0.500 (wt%) demonstrated excellent cell compatibility and increased the viability of NIH/3T3 (mouse embryo fibroblast) cells within 72 h. According to the results, Achillea lycaonica-loaded PLA nanofibers have proper tensile strength and controlled release. The working temperature range enlarged for the composites having higher plant extract content. Consequently, Achillea lycaonica-loaded nanofibrous mats have a great potential in skin tissue engineering applications.

International wound journal, Jan 27, 2018

Electrospun nanofibrous scaffolds are promising regenerative wound dressing options but have yet ... more Electrospun nanofibrous scaffolds are promising regenerative wound dressing options but have yet to be widely used in practice. The challenge is that nanofibre productions rely on bench-top apparatuses, and the delicate product integrity is hard to preserve before reaching the point of need. Timing is critically important to wound healing. The purpose of this investigation is to produce novel nanofibrous scaffolds using a portable, hand-held "gun", which enables production at the wound site in a time-dependent fashion, thereby preserving product integrity. We select bacterial cellulose, a natural hydrophilic biopolymer, and polycaprolactone, a synthetic hydrophobic polymer, to generate composite nanofibres that can tune the scaffold hydrophilicity, which strongly affects cell proliferation. Composite scaffolds made of 8 different ratios of bacterial cellulose and polycaprolactone were successfully electrospun. The morphological features and cell-scaffold interactions were ...

Journal of the Australian Ceramic Society, 2017

The biocompatibility of clinoptilolite/alumina/bovine hydroxyapatite (Cp-A1 2 O 3-BHA) composite,... more The biocompatibility of clinoptilolite/alumina/bovine hydroxyapatite (Cp-A1 2 O 3-BHA) composite, at different ratio obtained by powder pressing process, were investigated studying the behavior of osteosarcoma (SAOS-2) cells. The biocompatibility was examined by means of cytotoxicity and cytocompatibility tests. The structure and morphology of bioceramic composites were studied by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) technique. The results showed that these materials have no toxic effects. The natural composite that fabricated in this study may be a promising approach for boneengineering applications.

International Journal of Polymeric Materials and Polymeric Biomaterials, 2015

ABSTRACT In this study, we prepared photo-crosslinked modified HAP (hydroxy apatite)/polyvinyl al... more ABSTRACT In this study, we prepared photo-crosslinked modified HAP (hydroxy apatite)/polyvinyl alcohol (PVA) composite nanofiber scaffold for cell growth applications. HAP was synthesized and then modified with 4-vinylbenzene boronic acid (VBBA) to obtain 4-VBBA-HAP. By means of the simultaneous UV electrospinning technique 4-VBBA-HAP/PVA composite was obtained. The structure and morphology of electrospun membranes were investigated by scanning electron microscopy) and Fourier transform infrared spectroscopy technique. Nanofibers were treated with collagen solution via the spraying method. For the cell culture applications ECV304 and SAOS cells were seeded on the chosen electrospun fibrous scaffolds.

Macromolecular Materials and Engineering, 2018

Bacterial cellulose (BC) is a very promising biological material. However, at present its utiliza... more Bacterial cellulose (BC) is a very promising biological material. However, at present its utilization is limited by difficulties in shape forming it. In this Communication, it is shown how this can be overcome by blending it with poly(methylmethacrylate) (PMMA) polymer. BC:PMMA fibers are produced by pressurized gyration of blended BC:PMMA solutions. Subsequently, BC:PMMA bandage‐like scaffolds are generated with different blends. The products are investigated to determine their morphological and chemical features. Cell culture and proliferation tests are performed to obtain information on biocompatibility of the scaffolds.

The Journal of Advanced Prosthodontics, 2020

PURPOSE. The effects of four different self-adhesive resin cement materials on cell viability and... more PURPOSE. The effects of four different self-adhesive resin cement materials on cell viability and apoptosis after direct and indirect exposure were evaluated using different cell culture techniques. MATERIALS AND METHODS. Self-adhesive cements were applied to NIH/3T3 mouse fibroblasts by the extract test method, cell culture inserts, and dentin barrier test method. After exposure periods of 24 h and 72 h, the cytotoxicity of these self-adhesive materials was evaluated using the MTT assay (viability) and the Annexin-V-FITC/PI staining (apoptosis). RESULTS. The lowest cell viability was found in cells exposed to BeautiCem SA for 24 h in the extract test method. Cell viability was reduced to 70.6% compared to negative controls. After the 72 h exposure period, viability rate of cell cultures exposed to BeautiCem SA decreased more than 2-fold (29.5%) while cells exposed to RelyX U200 showed the highest viability rate of 71.4%. In the dentin barrier test method, BeautiCem SA induced the highest number of cells in apoptosis after a 24 h exposure (4.1%). Panavia SA Cement Plus was the material that caused the lowest number of cells in apoptosis (1.5%). CONCLUSION. The used self-adhesive cements have showed different cytotoxic effects based on the evaluation method. As exposure time increased, the materials showed more cytotoxic and apoptotic effects. BeautiCem SA caused significantly more severe cytotoxic and apoptotic effects than other cements tested. Moreover, cements other than BeautiCem SA have caused necrotic cell death rather than apoptotic cell death.

İstanbul Tıp Fakültesi Dergisi, 2019

The effects of zeolite supported nutrition on cancer development in the 1,2 dymethylhydrasin indu... more The effects of zeolite supported nutrition on cancer development in the 1,2 dymethylhydrasin induced-colon cancer model. J Ist Faculty Med.

Turkiye Klinikleri Journal of Medical Sciences, 2010

Alt ho ugh um bi li cal cord blo od (UCB) is a use ful so ur ce of stem and pro geni tor cells fo... more Alt ho ugh um bi li cal cord blo od (UCB) is a use ful so ur ce of stem and pro geni tor cells for bo ne mar row trans plan ta ti on, it is not pos sib le to ob ta in suf fi ci ent num ber of trans plantab le cells from a sing le hu man um bi li cal cord for trans plan ta ti on in adult pa ti ents. In this study, we com bi ned early and la te growth fac tors in short term cul tu res to en rich cord blo od CD34+ he ma topo i e tic pro ge ni tor cell co unt for adult pa ti ents. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : Cord blo od mo no nuc le ar cells (CBMC) har ves ted from 15 he althy vo lun te ers, de li ve ring in term, in cu ba ted in cul tu re me di um supp le men ted with in ter le u kin-3 (IL-3), IL-6, IL-11, stem cell fac tor, flt3/flk2 li gand and throm bo po i etin for 14 days. Star ting cells and cul tu red cells (day 7 and 14 cells) we re eva lu a ted for; CD4, CD8, CD33, CD34, CD38 and HLA-DR by flow cyto metry and se e ded in se mi so lid methy lcel lu lo se cul ture for de ter mi ning BFU-E, CFU-GM and CFU-GEMM co lony for ming abi li ti es. R Re e s su ul lt ts s: : In the shortterm cul tu res, to tal CBMC co unt sig ni fi cantly in cre a sed (p= 0.0001). Com pa red to star ting cells, the ex pres si ons of CD34 (p= 0.002), CD33 (p= 0.0001), HLA-DR (p= 0.0001) and CD8 (p= 0.002) in cre ased, but tho se of CD38 (p= 0.057) and CD4 (p= 0.0001) we re sup pres sed on day 14 of cul tu res. The re we re an in cre a se in ge ne ra ti on of CFU-GM (p= 0.131) and CFU-GEMM (p= 0.134) co lo ni es and a signi fi cant dec re a se in ge ne ra ti on of BFU-E co lo ni es (p= 0.0001). C Co on nc c l lu u s si i o on n: : Ex vi vo ex pan si on of CBMC, un der com bi na ti on of the growth fac tors, con tri bu te to CD34+ pro ge ni tor cell co unt and may help to re ach suf fi ci ent amo unt of trans plan tab le cells for adult pa ti ents. Sus pen si on cul tu res may lead to eli mi na ti on of graft-ver sus-host di se a se risk and pro tec ti on of graft-ver sus-le u ke mi a ef fect, through the dec re a se of CD4+ T cells and ma in te nan ce of CD8+ T lymphocy tes, res pec ti vely. K Ke ey y W Wo or rd ds s: : Cord blo od stem cell trans plan ta ti on; an ti gens, CD34; cell cul tu re tech ni qu es Ö ÖZ ZE ET T A Am ma aç ç: : Gö bek kor don ka nı kök ve pro je ni tör hüc re açı sın dan ke mik ili ği trans plan tas yo nu için el ve riş li bir kay nak ol ma sı na kar şın tek bir in san gö bek kor do nun dan eriş kin has ta la ra na kil için ye ter li sa yı da hüc re el de et me ola na ğı bu lun ma mak ta dır. Ça lış ma mız da kor don ka nı CD34+ he mopo e tik pro je ni tör hüc re le ri eriş kin has ta la ra ye te cek dü zey de ço ğalt mak ama cıy la er ken ve geç etki li bü yü me fak tör le ri ni kı sa sü re li kül tür ler de kom bi ne et tik. G Ge e r re eç ç v ve e Y Yö ön n t te em ml le er r: : Mi a dın da do ğum ya pan 15 sağ lık lı gö nül lü den kor don ka nı mo no nük le er hüc re le ri alı na rak in ter lö kin-3 (IL-3), IL-6, IL-11, stem cell fak tör, flt3/flk2 li gand and trom bo po e tin ek le nen kül tür med yu mun da 14 gün sü rey le in kü be edil di. Baş lan gıç hüc re ler ile kül tür den alı nan hüc re ler (7. ve 14. gün ler) flow si to met ri de CD4, CD8, CD33, CD34, CD38 ve HLA-DR yö nün den ana liz edil di ve ay nı za man da yarı ka tı me til se lü loz kül tür le re eki le rek BFU-E, CFU-GM ve CFU-GEMM ko lo ni le ri ni mey da na getir me ka pa si te le ri in ce len di. B Bu ul l g gu u l la ar r: : Kı sa sü re li kül tür ler de to tal kor don ka nı mo no nük le er hüc re sa yı sın da an lam lı ar tış göz len di (p= 0.0001). Baş lan gıç hüc re le ri ile kar şı laş tı rıl dı ğın da, kül tür le rin 14. gü nün de hüc re ler de CD34 (p= 0.002), CD33 (p= 0.0001) HLA-DR (p= 0.0001) ve CD8 (p= 0.002) eks pres yon la rın da ar tış la bir lik te CD38 (p= 0.057) ve CD4 (p= 0.0001) eks pres yo nun da bas kı lan ma göz len di. CFU-GM (p= 0.131) ve CFU-GEMM (p= 0.134) ko lo ni le ri ni oluş tur ma ka pa si te le rin de artış ve BFU-E (p= 0.0001) ko lo ni oluş tur ma ka pa si te le rin de an lam lı dü şüş göz len di. S So o n nu uç ç: : Bü yü me fak tör le ri nin kom bi nas yo nu al tın da kor don ka nı mo no nük le er hüc re le ri nin vü cut dı şı or tam da çoğal tıl ma sı, eriş kin has ta la ra ye te cek mik tar da nak le di le bi lir CD34+ pro je ni tör hüc re sa yı sı na ulaşma ya yar dım cı ola bi le cek tir. Süs pan si yon kül tü rü son ra sı CD4+ T hüc re sa yı sın da ki azal ma graft-ver sus-host has ta lı ğı ris ki nin or ta dan kal dı rıl ma sı na yar dım cı ola bi le cek ve CD8+ T len fo sitle ri nin ya şam la rı nı sür dür me le ri ile de graf tın an ti-lö se mik et ki si ko ru na bi le cek tir. A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Kor don ka nı kök hüc re nak li; an ti jen ler, CD34; hüc re kül tü rü tek nik le ri T Tu ur rk ki iy ye e K Kl li in ni ik kl le er ri i J J M Me ed d S Sc ci i 2 20 01 10 0; ;3 30 0((1 1

Hormone and Metabolic Research, 2006

This study was performed to compare the effects of 17beta-estradiol on blood-brain barrier disrup... more This study was performed to compare the effects of 17beta-estradiol on blood-brain barrier disruption in focal cerebral ischemia between younger and older rats. Younger (three-month-old) and older (24-month-old) ovariectomized female Fischer 344 rats were studied. In one half of each age group, a 500 microg 17beta-estradiol 21-day release pellet and in another half, a vehicle pellet was implanted 21 days before the experiments. One hour after middle cerebral artery occlusion, the transfer coefficient (Ki) of 14C-alpha-aminoisobutyric acid and the volume of 3H-dextran distribution were determined to examine the degree of blood-brain barrier disruption. In all four groups, the Ki in the ischemic cortex was higher than in the corresponding contralateral cortex. There was no significant difference in the Ki in both cortices among the groups. The volume of dextran distribution of the ischemic cortex was only greater than in the corresponding contralateral cortex in the older 17beta-estradiol-treated group, and the volume of that group was greater than the younger 17beta-estradiol-treated group (4.00 +/- 1.29 VS. 2.13 +/- 0.88 ml/100 g). After analyzing the difference in Ki between the ischemic cortex and the contralateral cortex in each animal, the difference was significantly greater in the older 17beta-estradiol-treated rats than the older vehicle-treated rats (3.40 +/- 2.10 VS. 1.26 +/- 1.44 microl/g/min). In the younger rats, however, 17beta-estradiol did not significantly affect the difference. Our data showed that 17beta-estradiol treatment failed to attenuate the BBB disruption in the cerebral ischemic cortex in the older or younger Fischer 344 rats. However, our data also suggest the possibility that 17beta-estradiol could aggravate the BBB disruption in older rats.

Glokom- …, 2009

1 Bağcılar EA Hastanesi, Göz Kliniği, İstanbul, Uzm. Dr. 2 İ.Ü, İstanbul Tıp Fakültesi, Fizyoloji... more 1 Bağcılar EA Hastanesi, Göz Kliniği, İstanbul, Uzm. Dr. 2 İ.Ü, İstanbul Tıp Fakültesi, Fizyoloji AD, İstanbul, Prof. Dr. 3 İ.Ü, Fen Fakültesi, Biyoloji Bölümü, İstanbul, Uzm. Dr. 4 İ.Ü., İstanbul Tıp Fakültesi, Fizyoloji AD, İstanbul, Asist. Dr. 5 İ.Ü., Fen Fakültesi, Moleküler Biyoloji ve ...

Istanbul Yeni Yuzyil Universitesi, Yeni Yuzyil Journal of Medical Sciences

Meme kanseri, kadınlarda en yaygın görülen kanserlerden biridir ve dünyadaki en yüksek insidansa ... more Meme kanseri, kadınlarda en yaygın görülen kanserlerden biridir ve dünyadaki en yüksek insidansa sahip kanser türüdür. Meme kanseri gelişiminde cinsiyet, yaş, östrojen düzeyi, aile öyküsü, gen mutasyon- ları ve sağlıksız yaşam tarzı gibi çok sayıda risk faktörü bulunmaktadır. Mevcut tedavi seçenekleri cerrahi, radyoterapi, hormonal tedaviler, kemoterapi ve hedefe yönelik tedavilerdir. Hedefe yönelik tedavilerin içinde immünoterapi son yıllarda oldukça yaygın bir tedavi stratejisi olarak karşımıza çıkmaktadır. Meme kanseri immün sistemden bağımsız bir kanser olduğu düşün- cesinin aksine, artık meme kanserinin immün temelli bir kanser türü olduğu bilinmektedir. Bu kapsamda diğer kanser türlerinde olduğu gibi, meme kanserinde de immünoterapinin hedeflediği moleküller- den programlanmış hücre ölümü-1 (PD-1) ve programlanmış hücre ölümü ligandı-1 (PD-L1) ile yapılan çok sayıda çalışma bulunmakta- dır. PD-1 ve PD-L1 hedefli tedaviler günümüzde meme kanseri tedavi- sinde uygulanmaktadır. M...

Molecular Biology Reports

International Journal of Biological Macromolecules

1,25(OH)2D3 (Calcitriol, active form of Vitamin D), which is a broad regulatory molecule, plays a... more 1,25(OH)2D3 (Calcitriol, active form of Vitamin D), which is a broad regulatory molecule, plays a role in changing the efficacy of chemotherapeutic drugs. In bladder cancer, effectiveness of the treatment and reducing the resistance to chemotherapeutics such as cisplatin are of great importance. Cisplatin usage is one of a current standard chemotherapy regimen for bladder cancer. In our study T24, ECV-304 and HUVEC cell lines were treated with calcitriol and cisplatin individually and with combination. MTT assays were used for optimal dose determination. It was aimed to determine the possible potential of the combined use of cisplatin with calcitriol. Determined doses and combination treatments of calcitriol and cisplatin on all cell lines were evaluated for cytotoxicity, apoptosis and drug resistance. Also calcitriol and cisplatin interaction with p53 protein was assessed by molecular docking analysis. Datas showed that combination treatment increased the anti-proliferative efficac...

are both associated with a splenomegaly, an extreme hyper-leukocytosis and a shorter complete rem... more are both associated with a splenomegaly, an extreme hyper-leukocytosis and a shorter complete remission duration. The median overall survival and event free survival were respectively of 8 months and 4 months. The age (<1 year and >50 yeras) and the WBC (>30.109 / l) are unfavorable predictive prognostic factors. Translocation t(4; 11) is associated to the worst clinical outcome. In conclusion, 11q23 rearrangements in hematologi-cal malignancies are associated to heterogeneous clinical features and treatment outcome according to the cytogenetic subroup. Abstract: 187 Oral: 187 Abstract: 204 Poster: 111

Istanbul Journal of Pharmacy, 2018

Macromolecular Materials and Engineering, 2018

Asian Pacific Journal of Cancer Prevention, 2020

ChemistrySelect, 2019

European Polymer Journal, 2019

International wound journal, Jan 27, 2018

Journal of the Australian Ceramic Society, 2017

International Journal of Polymeric Materials and Polymeric Biomaterials, 2015

Macromolecular Materials and Engineering, 2018

The Journal of Advanced Prosthodontics, 2020

İstanbul Tıp Fakültesi Dergisi, 2019

Turkiye Klinikleri Journal of Medical Sciences, 2010

Hormone and Metabolic Research, 2006

Glokom- …, 2009

Istanbul Yeni Yuzyil Universitesi, Yeni Yuzyil Journal of Medical Sciences

Molecular Biology Reports

International Journal of Biological Macromolecules