S. Pourbakhsh - Academia.edu (original) (raw)

Papers by S. Pourbakhsh

Iranian Journal of Veterinary Medicine, 2015

Background: Campylobacter jejuni and Campylobacter coli are zoonotic bacteria which are frequentl... more Background: Campylobacter jejuni and Campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. Sharing of the cytolethal distending toxin (cdt) genes in Campylobacter is common and is considered species specific. OBJECTIVES: In this study we focused on detecting the presence of cdt gene in C. jejuni and C. coli isolated from broilers, turkeys and quails of Iran. METHODS: Cecal samples were randomly collected from 240 broiler chickens, 100 meat type turkeys and 100 quails after slaughtering. We used PCR as a method for detecting cdt genes. RESULTS: In broilers, 93% of 58 C. jejuni positive samples possessed cdt gene and in all cases the three different subunits of cdt genes were present. However, only 56% of 14 C. coli isolates in broilers had contained cdt genes, while one fourth having all three subunits present. In turkeys, around 65% of 34 C. jejuni positive samples had cdt gene present with 38% possessing all three subunits of cdt genes. But a...

A mannose-resistant hemagglutinating (MRHA) P or P-related fimbriae was purified from three Ecoli... more A mannose-resistant hemagglutinating (MRHA) P or P-related fimbriae was purified from three Ecoli isolates from chickens with colisepticemia. The polycJonal antiserum against the fimbriae was prepared in rabbits. Examination of the purified fimbriae by SOS-polyacrylamide gel electrophoresis (SOS-PAGE) demonstrated a major fimbriae subunit of approximately 18 kOa. This band was also reacted with anti-FII serum on Western blotting. The antiserum against the avian P fimbriae strongly reacted with the major subunit of the homologous fimbriae and with Fil fimbriae on Western blotting. The first 21 N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriae was identical to that of Fil fimbriae. The adhesive properties of the avian P fimbriae was similar to that of Fil fimbriae with regard to both MRHA ofhuman erythrocytes and binding to the Gal-Gal cJass II receptor. These results indicate that this fimbriae on avian Ecoli isolates are cJosely related to Fil fimb...

An indirect enzyme-Iinked immunosorbent assay (ELISA) was developed for the rapid and efficient l... more An indirect enzyme-Iinked immunosorbent assay (ELISA) was developed for the rapid and efficient large scale screening of antibodies to avian influenza virus (AIV) infection in chicken. Antigen was a whole-purified influenza virus produced from the H9N2 subtype. Optimum dilution for goat anti-chicken conjugate to be used in the ELISA was 1: 1000, as determined by signal-to-noise ratio. The antigen concentration was 0.375J.1g of protein per weil, as determined by checkerboard titration. The sensitivity of the ELISA was compared with hemmaglutination inhibition test under field exposure. After testing of 656 field sera, the correlation coefficient for the results of two tests was significant (r=0.929, P<O.OOI). Testing 8 standard antisera of various subtypes (HI, H2, H4, H5, H6, H7, H9 and HIO) of AIV and AIV antibody positive and negative sera determined specificity of the ELISA. Antisera to ail 8-hemmaglutinin subtypes were strongly positive. 80th the sensitivity and specificity o...

Ten Hybridomas secreting monoclonal antibodies directed against the Fglycoprotein of MKI3 (Irania... more Ten Hybridomas secreting monoclonal antibodies directed against the Fglycoprotein of MKI3 (Iranian isolate) strain of Newcastle disease virus (NOV) have isolated which 6 of them showed positive reaction in different tests. Ali of these antibodies are IgM c1ass with K .chain which showed sharp and single band on F-Protein (56000 Dalton) in western blot assay. Ali these antibodies reacted with same epitope on competitive ELISA. On cross reactivity analysis with different avian viruses ail were specifie to NOV only and did not show any reaction with other virus. The importance and application of these antibodies would be on specifie identification of NOV. highly purification of F-protein of NOV specially on preparation of recombinant F-protein on subunit vaccine, developing anti-idiotype vaccine and al50 blocking the c1eavage site of F-protein which resulting in decreasing pathogenicity of virus.

A total of twelve Newcastle disease virus isolates recovered from different outbreaks in Iran wer... more A total of twelve Newcastle disease virus isolates recovered from different outbreaks in Iran were analyzed for in vivo pathogenicity, biological property and amplification of F c1eavage site gene. Intracerebral pathogenicity index varied from 1.7 to 1.96 while the intravenous pathogenicity index was more than 2.0. The me an death time value for ail the isolates ranged from 39.6 to 68.8 hours. Ali the isolates except 2, hemagglutinated bovine erythrocytes whereas only one of the isolates agglutinated the equine erythrocytes. Polymerase chain reaction was used to amplify the F c1eavage site gene of ail isolates. Based on me an death time the Newcastle disease virus isolates could be placed in mesogenic or velogenic group.

Abstract: Vaccination is often considered as an appropriate option in prevention most of poultry ... more Abstract: Vaccination is often considered as an appropriate option in prevention most of poultry viral diseases worldwide. This study was conducted to evaluate effects of current routine vaccination routes (spray, eye-drop and drinking water) of live vaccines against infectious bronchitis (IB) on performance and humoral immune responses of broiler chickens. The results of this study indicated that Vaccination significantly (P<0.05) affects performance of the broiler chickens and effects on weight gain and FCR, did not differed significantly among these routes. Immune responses of vaccinated chickens were significantly (P<0.001) differed from those of control chickens. Comparison of various vaccination routes revealed that eye-drop group had the highest antibody titer with the closest range. There were also positive significant (P<0. 01) degrees of correlation among chickens vaccinated with spray and eye-drop, spray and drinking water, eye-drop and drinking water (r = 0.84, ...

Abstract: The in vitro activities of aivlosin tylosin tiamulin, erythromycin, oxytetracycline, sp... more Abstract: The in vitro activities of aivlosin tylosin tiamulin, erythromycin, oxytetracycline, spectinomycin, lincomycin, lincomycin-spectinomycin (1:2) and enrofloxacin were determined against twenty-eight isolates of Mycoplasma gallisepticum and 4 isolates of Mycoplasma synoviae using microbroth dilution method. The isolates showed various susceptibilities to antimicrobial agents. Aivlosin, lincomycin-spectinomycin (1:2), tylosin, tiamulin, enrofloxacin and also lincomycin were commonly more effective against these field isolates. However, aivlosin was the most effective drugs against Mycoplasma gallisepticum and Mycoplasma synoviae as its MIC was the lowest of all. Oxytetracycline, erythromycin and spectinomycin were not effective against all isolates, as their MICs for some of isolates were extremely high. The MIC of erythromycin and oxytetracycline were distributed across a broad range. Resistant strains to two mentioned antibiotics were obtained from the field. Key words: Myco...

Summary The type, severity and frequency of gross lesion, histopatholgic change, and tissue tropi... more Summary The type, severity and frequency of gross lesion, histopatholgic change, and tissue tropism of A/Chicken/Iran/259/1998(H9N2) avian influenza virus following intratrachealy (IT) inoculation were studied in chickens. Twenty, 5-week-old chickens (hatched from SPF eggs) were inoculated with this virus. Another twenty chickens were inoculated with sterile chorio allantoic fluid. Tracheitis, pneumonia and tubulointerstital nephritis were the most frequent specific histopathologic changes. Influenza nucleoproteins were demonstrated in epithelium of trachea, secondary bronchi and cecal tonsile of an inoculated chicken. Common non-specific histopathologic changes were lymphoid and reticuloendothelial cell hyperplasia in spleen, cecal tonsil and leukocyte cell infiltration in myocardium. These results indicate that the low-pathogenic avian influenza virus is epithliotropic in chicken. In IT route of inoculation, it has tissue tropism and pathogenicity for trachea, lung (pneumotropic) ...

A mannose-resistanthemagglutinating(MRHA)P or P-relatedfimbriae was purified from three E.coli is... more A mannose-resistanthemagglutinating(MRHA)P or P-relatedfimbriae was purified from three E.coli isolates from chickenswith colisepticemia.The polyclonal antiserum against the fimbriae was prepared in rabbits. Examination of the purified fimbriae by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated a major fimbriae subunit of approximately 18 kDa. This band was also reacted with anti-FII serum on Western blotting. The antiserum against the avian P fimbriae strongly reacted with the major subunit of the homologousfimbriae and with FII fimbriae on Western blotting. The first 21 N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriaewas identical to that of FII fimbriae. The adhesive properties of the avian P fimbriae was similar to that of FII fimbriae with regard to both MRHA of human erythrocytes and binding to the Gal-Gal class 11receptor. These results indicate that this fimbriae on avian E.coli isolates are closely related to FII fimbriae which are associated with E.coli isolatedfrom human urinarytract infection.

archives of razi institute, 2000

Summary Four antigens consisting of heat killed cells, formalin killed cells, lipopolysaccharides... more Summary Four antigens consisting of heat killed cells, formalin killed cells, lipopolysaccharides and extracellular products were prepared from Aeromonas hydrophila. The immune response of about 3-year-old Persian sturgeon (Acipenser persicus) that immunized by intraperitoneally injection of the antigens with or without Freund’s adjuvant was evaluated. The immune response to the extracellular products was significantly (P<0.05) less than that other antigens. The results indicate that whole cell antigens of A.hydrophila represent protective antigens against motile aeromonad septicemia.

Summary A total of twelve Newcastle disease virus isolates recovered from different outbreaks in ... more Summary A total of twelve Newcastle disease virus isolates recovered from different outbreaks in Iran were analyzed for in vivo pathogenicity, biological property and amplification of F cleavage site gene. Intracerebral pathogenicity index varied from 1.7 to 1.96 while the intravenous pathogenicity index was more than 2.0. The mean death time value for all the isolates ranged from 39.6 to 68.8 hours. All the isolates except 2, hemagglutinated bovine erythrocytes whereas only one of the isolates agglutinated the equine erythrocytes. Polymerase chain reaction was used to amplify the F cleavage site gene of all isolates. Based on mean death time the Newcastle disease virus isolates could be placed in mesogenic or velogenic group.

Archives of Razi Institute, 2017

Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious ag... more Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefaciens (Mp) produce a clinically similar disease, more often in goats. The aim of the present study was to detect Mcc in sheep flocks in East Azerbaijan Province of Iran. Milk, ear canal, and eye swab samples were collected from 49 sheep flocks with clinical signs of CA or a history of a disease. All the samples were examined using both culture and molecular methods. In the molecular method,positive samples for the Mycoplasma genus were tested for M. mycoides cluster and Mcc. From 272 samples, 67, 87, and 62 samples were shown to be positive using the culture method, polymerase chain reaction (PCR...

Iranian Journal of Veterinary Medicine, 2015

Background: Campylobacter jejuni and Campylobacter coli are zoonotic bacteria which are frequentl... more Background: Campylobacter jejuni and Campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. Sharing of the cytolethal distending toxin (cdt) genes in Campylobacter is common and is considered species specific. OBJECTIVES: In this study we focused on detecting the presence of cdt gene in C. jejuni and C. coli isolated from broilers, turkeys and quails of Iran. METHODS: Cecal samples were randomly collected from 240 broiler chickens, 100 meat type turkeys and 100 quails after slaughtering. We used PCR as a method for detecting cdt genes. RESULTS: In broilers, 93% of 58 C. jejuni positive samples possessed cdt gene and in all cases the three different subunits of cdt genes were present. However, only 56% of 14 C. coli isolates in broilers had contained cdt genes, while one fourth having all three subunits present. In turkeys, around 65% of 34 C. jejuni positive samples had cdt gene present with 38% possessing all three subunits of cdt genes. But a...

A mannose-resistant hemagglutinating (MRHA) P or P-related fimbriae was purified from three Ecoli... more A mannose-resistant hemagglutinating (MRHA) P or P-related fimbriae was purified from three Ecoli isolates from chickens with colisepticemia. The polycJonal antiserum against the fimbriae was prepared in rabbits. Examination of the purified fimbriae by SOS-polyacrylamide gel electrophoresis (SOS-PAGE) demonstrated a major fimbriae subunit of approximately 18 kOa. This band was also reacted with anti-FII serum on Western blotting. The antiserum against the avian P fimbriae strongly reacted with the major subunit of the homologous fimbriae and with Fil fimbriae on Western blotting. The first 21 N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriae was identical to that of Fil fimbriae. The adhesive properties of the avian P fimbriae was similar to that of Fil fimbriae with regard to both MRHA ofhuman erythrocytes and binding to the Gal-Gal cJass II receptor. These results indicate that this fimbriae on avian Ecoli isolates are cJosely related to Fil fimb...

An indirect enzyme-Iinked immunosorbent assay (ELISA) was developed for the rapid and efficient l... more An indirect enzyme-Iinked immunosorbent assay (ELISA) was developed for the rapid and efficient large scale screening of antibodies to avian influenza virus (AIV) infection in chicken. Antigen was a whole-purified influenza virus produced from the H9N2 subtype. Optimum dilution for goat anti-chicken conjugate to be used in the ELISA was 1: 1000, as determined by signal-to-noise ratio. The antigen concentration was 0.375J.1g of protein per weil, as determined by checkerboard titration. The sensitivity of the ELISA was compared with hemmaglutination inhibition test under field exposure. After testing of 656 field sera, the correlation coefficient for the results of two tests was significant (r=0.929, P<O.OOI). Testing 8 standard antisera of various subtypes (HI, H2, H4, H5, H6, H7, H9 and HIO) of AIV and AIV antibody positive and negative sera determined specificity of the ELISA. Antisera to ail 8-hemmaglutinin subtypes were strongly positive. 80th the sensitivity and specificity o...

Ten Hybridomas secreting monoclonal antibodies directed against the Fglycoprotein of MKI3 (Irania... more Ten Hybridomas secreting monoclonal antibodies directed against the Fglycoprotein of MKI3 (Iranian isolate) strain of Newcastle disease virus (NOV) have isolated which 6 of them showed positive reaction in different tests. Ali of these antibodies are IgM c1ass with K .chain which showed sharp and single band on F-Protein (56000 Dalton) in western blot assay. Ali these antibodies reacted with same epitope on competitive ELISA. On cross reactivity analysis with different avian viruses ail were specifie to NOV only and did not show any reaction with other virus. The importance and application of these antibodies would be on specifie identification of NOV. highly purification of F-protein of NOV specially on preparation of recombinant F-protein on subunit vaccine, developing anti-idiotype vaccine and al50 blocking the c1eavage site of F-protein which resulting in decreasing pathogenicity of virus.

A total of twelve Newcastle disease virus isolates recovered from different outbreaks in Iran wer... more A total of twelve Newcastle disease virus isolates recovered from different outbreaks in Iran were analyzed for in vivo pathogenicity, biological property and amplification of F c1eavage site gene. Intracerebral pathogenicity index varied from 1.7 to 1.96 while the intravenous pathogenicity index was more than 2.0. The me an death time value for ail the isolates ranged from 39.6 to 68.8 hours. Ali the isolates except 2, hemagglutinated bovine erythrocytes whereas only one of the isolates agglutinated the equine erythrocytes. Polymerase chain reaction was used to amplify the F c1eavage site gene of ail isolates. Based on me an death time the Newcastle disease virus isolates could be placed in mesogenic or velogenic group.

Abstract: Vaccination is often considered as an appropriate option in prevention most of poultry ... more Abstract: Vaccination is often considered as an appropriate option in prevention most of poultry viral diseases worldwide. This study was conducted to evaluate effects of current routine vaccination routes (spray, eye-drop and drinking water) of live vaccines against infectious bronchitis (IB) on performance and humoral immune responses of broiler chickens. The results of this study indicated that Vaccination significantly (P<0.05) affects performance of the broiler chickens and effects on weight gain and FCR, did not differed significantly among these routes. Immune responses of vaccinated chickens were significantly (P<0.001) differed from those of control chickens. Comparison of various vaccination routes revealed that eye-drop group had the highest antibody titer with the closest range. There were also positive significant (P<0. 01) degrees of correlation among chickens vaccinated with spray and eye-drop, spray and drinking water, eye-drop and drinking water (r = 0.84, ...

Abstract: The in vitro activities of aivlosin tylosin tiamulin, erythromycin, oxytetracycline, sp... more Abstract: The in vitro activities of aivlosin tylosin tiamulin, erythromycin, oxytetracycline, spectinomycin, lincomycin, lincomycin-spectinomycin (1:2) and enrofloxacin were determined against twenty-eight isolates of Mycoplasma gallisepticum and 4 isolates of Mycoplasma synoviae using microbroth dilution method. The isolates showed various susceptibilities to antimicrobial agents. Aivlosin, lincomycin-spectinomycin (1:2), tylosin, tiamulin, enrofloxacin and also lincomycin were commonly more effective against these field isolates. However, aivlosin was the most effective drugs against Mycoplasma gallisepticum and Mycoplasma synoviae as its MIC was the lowest of all. Oxytetracycline, erythromycin and spectinomycin were not effective against all isolates, as their MICs for some of isolates were extremely high. The MIC of erythromycin and oxytetracycline were distributed across a broad range. Resistant strains to two mentioned antibiotics were obtained from the field. Key words: Myco...

Summary The type, severity and frequency of gross lesion, histopatholgic change, and tissue tropi... more Summary The type, severity and frequency of gross lesion, histopatholgic change, and tissue tropism of A/Chicken/Iran/259/1998(H9N2) avian influenza virus following intratrachealy (IT) inoculation were studied in chickens. Twenty, 5-week-old chickens (hatched from SPF eggs) were inoculated with this virus. Another twenty chickens were inoculated with sterile chorio allantoic fluid. Tracheitis, pneumonia and tubulointerstital nephritis were the most frequent specific histopathologic changes. Influenza nucleoproteins were demonstrated in epithelium of trachea, secondary bronchi and cecal tonsile of an inoculated chicken. Common non-specific histopathologic changes were lymphoid and reticuloendothelial cell hyperplasia in spleen, cecal tonsil and leukocyte cell infiltration in myocardium. These results indicate that the low-pathogenic avian influenza virus is epithliotropic in chicken. In IT route of inoculation, it has tissue tropism and pathogenicity for trachea, lung (pneumotropic) ...

A mannose-resistanthemagglutinating(MRHA)P or P-relatedfimbriae was purified from three E.coli is... more A mannose-resistanthemagglutinating(MRHA)P or P-relatedfimbriae was purified from three E.coli isolates from chickenswith colisepticemia.The polyclonal antiserum against the fimbriae was prepared in rabbits. Examination of the purified fimbriae by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated a major fimbriae subunit of approximately 18 kDa. This band was also reacted with anti-FII serum on Western blotting. The antiserum against the avian P fimbriae strongly reacted with the major subunit of the homologousfimbriae and with FII fimbriae on Western blotting. The first 21 N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriaewas identical to that of FII fimbriae. The adhesive properties of the avian P fimbriae was similar to that of FII fimbriae with regard to both MRHA of human erythrocytes and binding to the Gal-Gal class 11receptor. These results indicate that this fimbriae on avian E.coli isolates are closely related to FII fimbriae which are associated with E.coli isolatedfrom human urinarytract infection.

archives of razi institute, 2000

Summary Four antigens consisting of heat killed cells, formalin killed cells, lipopolysaccharides... more Summary Four antigens consisting of heat killed cells, formalin killed cells, lipopolysaccharides and extracellular products were prepared from Aeromonas hydrophila. The immune response of about 3-year-old Persian sturgeon (Acipenser persicus) that immunized by intraperitoneally injection of the antigens with or without Freund’s adjuvant was evaluated. The immune response to the extracellular products was significantly (P<0.05) less than that other antigens. The results indicate that whole cell antigens of A.hydrophila represent protective antigens against motile aeromonad septicemia.

Summary A total of twelve Newcastle disease virus isolates recovered from different outbreaks in ... more Summary A total of twelve Newcastle disease virus isolates recovered from different outbreaks in Iran were analyzed for in vivo pathogenicity, biological property and amplification of F cleavage site gene. Intracerebral pathogenicity index varied from 1.7 to 1.96 while the intravenous pathogenicity index was more than 2.0. The mean death time value for all the isolates ranged from 39.6 to 68.8 hours. All the isolates except 2, hemagglutinated bovine erythrocytes whereas only one of the isolates agglutinated the equine erythrocytes. Polymerase chain reaction was used to amplify the F cleavage site gene of all isolates. Based on mean death time the Newcastle disease virus isolates could be placed in mesogenic or velogenic group.

Archives of Razi Institute, 2017

Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious ag... more Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefaciens (Mp) produce a clinically similar disease, more often in goats. The aim of the present study was to detect Mcc in sheep flocks in East Azerbaijan Province of Iran. Milk, ear canal, and eye swab samples were collected from 49 sheep flocks with clinical signs of CA or a history of a disease. All the samples were examined using both culture and molecular methods. In the molecular method,positive samples for the Mycoplasma genus were tested for M. mycoides cluster and Mcc. From 272 samples, 67, 87, and 62 samples were shown to be positive using the culture method, polymerase chain reaction (PCR...