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S Singh

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Papers by S Singh

RSC Advances, 2015

Zn2+ (0, 2, 5, 7 and 10 at%) co-doped CaMoO4:2Eu3+ nanophosphors have been synthesized via the po... more Zn2+ (0, 2, 5, 7 and 10 at%) co-doped CaMoO4:2Eu3+ nanophosphors have been synthesized via the polyol method using ethylene glycol (EG) as both capping agent and reaction medium at 150 °C.

2005 13th IEEE International Conference on Networks Jointly held with the 2005 IEEE 7th Malaysia International Conf on Communic, 2005

This paper describes an architecture that filters packets within a subset of nodes on an existing... more This paper describes an architecture that filters packets within a subset of nodes on an existing shared multicast tree. The path connecting the group of nodes that want to communicate privately on the existing tree will be given a label. These labels are used to route one-to-one and group communication traffic for selected nodes on a multicast tree. Nodes connected to the tree but are not on this label path will not receive any filtered packets. This filter architecture reduces network resource waste by utilising the existing network resources on the multicast tree like quality of service (QoS). In this paper, we also describe how this architecture can be used for mobile communication when implemented in a shared tree mobile multicast architecture.

Microorganisms in Sustainable Agriculture and Biotechnology, 2011

Agro residues, a precious source of nutrients are being disposed off because of their bulky natur... more Agro residues, a precious source of nutrients are being disposed off because of their bulky nature and non-availability of management practices. Besides loss of essential plant nutrients and organic matter important for sustainability of the system, it also causes ...

PLoS ONE, 2010

Understanding the requirements for protection against pneumococcal carriage and pneumonia will gr... more Understanding the requirements for protection against pneumococcal carriage and pneumonia will greatly benefit efforts in controlling these diseases. Several proteins and polysaccharide capsule have recently been implicated in the virulence of and protective immunity against Streptococcus pneumonia. Pneumococcal surface protein A (PspA) is highly conserved among S. pneumonia strains, inhibits complement activation, binds lactoferrin, elicits protective systemic immunity against pneumococcal infection, and is necessary for full pneumococcal virulence. Identification of PspA peptides that optimally bind human leukocyte antigen (HLA) would greatly contribute to global vaccine efforts, but this is hindered by the multitude of HLA polymorphisms. Here, we have used an experimental data set of 54 PspA peptides and in silico methods to predict peptide binding to HLA and murine major histocompatibility complex (MHC) class II. We also characterized spleenand cervical lymph node (CLN)-derived helper T lymphocyte (HTL) cytokine responses to these peptides after S. pneumonia strain EF3030-challenge in mice. Individual, yet overlapping peptides, 15 amino acids in length revealed residues 199 to 246 of PspA (PspA 199-246) consistently caused the greatest IFN-c, IL-2, IL-5 and proliferation as well as moderate IL-10 and IL-4 responses by ex vivo stimulated splenic and CLN CD4 + T cells isolated from S. pneumonia strain EF3030-challeged F 1 (B66BALB/c) mice. IEDB, RANKPEP, SVMHC, MHCPred, and SYFPEITHI in silico analysis tools revealed peptides in PspA 199-246 also interact with a broad range of HLA-DR,-DQ, and-DP allelles. These data suggest that predicted MHC class II-peptide binding affinities do not always correlate with T helper (Th) cytokine or proliferative responses to PspA peptides, but when used together with in vivo validation can be a useful tool to choose candidate pneumococcal HTL epitopes.