Suparat Srithawong - Academia.edu (original) (raw)

Papers by Suparat Srithawong

Research paper thumbnail of Genetic Structure and Forensic Utility of 23 Autosomal STRs of the Ethnic Lao Groups From Laos and Thailand

Frontiers in Genetics

The Lao Isan and Laotian are the major groups in the area of present-day northeastern Thailand an... more The Lao Isan and Laotian are the major groups in the area of present-day northeastern Thailand and Laos, respectively. Several previous genetic and forensic studies indicated an admixed genetic structure of Lao Isan with the local Austroasiatic speaking groups, e.g. Khmer, whereas there is a paucity of reporting Laotian’s forensic short tandem repeats (STRs). Here, we newly generated 451 genotypes of seven Lao Isan and three Laotian populations (two Lao Lum and one Lao Thoeng) using 23 autosomal STRs embedded in VerifilerTM plus PCR Amplification kit. We reported allelic frequency and forensic parameters in different dataset: combined ethnic Lao groups, combined Lao Isan populations and combined Laotians. Overall, the forensic parameter results indicate that this set of STRs is suitable for forensic investigation. The anthropological results revealed the genetic homogeneity of Tai-Kadai speaking Lao groups from Thailand and Laos, consistent with previous studies, while the Austroasi...

Research paper thumbnail of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

BMC Plant Biology

Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season... more Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season grasses. Erianthus species are widely distributed throughout Southeast Asia, East Asia and South Asia. Erianthus arundinaceus (Retz.) Jeswiet is highly adaptable to the environment, has a high percentage of dry matter, and is highly productive. Recently, this species has attracted attention as a novel bioenergy crop and as a breeding material for sugarcane improvement. Such interest in E. arundinaceus has accelerated the collection and conservation of its genetic resources, mainly in Asian countries, and also evaluation of morphological, agricultural, and cytogenetic features in germplasm collections. In Thailand, genetic resources of E. arundinaceus have been collected over the past 20 years and their phenotypic traits have been evaluated. However, the genetic differences and relatedness of the germplasms are not fully understood. Results A set of 41 primer pairs for nuclear simple seq...

Research paper thumbnail of Additional file 6 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 6: Figure S1. Geographic locations of 121 Erianthus accessions collected in Thail... more Additional file 6: Figure S1. Geographic locations of 121 Erianthus accessions collected in Thailand, and a pie chart of the populations and a bar chart of ancestry proportion in the 7 admixtures. Colors correspond to those in Fig. 1 at K = 4. Admixture group is indicated in gray. Accession numbers are listed as map No. in Table S2.

Research paper thumbnail of Additional file 5 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 5: Table S5. Sequences of primers used for amplification and sequencing of chloro... more Additional file 5: Table S5. Sequences of primers used for amplification and sequencing of chloroplast DNA regions in Thai Erianthus accessions.

Research paper thumbnail of Additional file 3 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 3: Table S3. GenBank accession numbers of three non-coding regions of chloroplast... more Additional file 3: Table S3. GenBank accession numbers of three non-coding regions of chloroplast DNA and their concatenated sequences in 121 Thai Erianthus accessions.

Research paper thumbnail of Additional file 4 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 4: Table S4. Statistical analysis of haplotype diversity, nucleotide diversity, a... more Additional file 4: Table S4. Statistical analysis of haplotype diversity, nucleotide diversity, and neutrality in each chloroplast DNA region in 121 Thai Erianthus accessions.

Research paper thumbnail of Additional file 2 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 2: Table S2. Sampling locations of 121 Erianthus accessions collected in Thailand... more Additional file 2: Table S2. Sampling locations of 121 Erianthus accessions collected in Thailand, their grouping by each analysis, and size of amplified fragment in each accession.

Research paper thumbnail of Additional file 1 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 1: Table S1. Characteristics of 28 SSR loci in 121 Thai Erianthus accessions.

Research paper thumbnail of Autosomal Microsatellite Investigation Reveals Multiple Genetic Components of the Highlanders from Thailand

Genes, 2021

The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiati... more The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiatic-speaking (AA) Khmu, Htin and Lawa; the Hmong-Mien-speaking (HM) IuMien and Hmong; and the Sino-Tibetan-speaking (ST) Akha, Karen, Lahu and Lisu. Except the Lawa, the rest of the hill tribes migrated into their present habitats only very recently. The Thai hill tribes were of much interest to research groups focusing on study of cultural and genetic variation because of their unique languages and cultures. So far, there have been several genetic studies of the Thai hill tribes. However, complete forensic microsatellite database of the Thai hill tribes is still lacking. To construct such database, we newly generated 654 genotypes of 15 microsatellites commonly used in forensic investigation that belong to all the nine hill tribes and also non-hill tribe highlanders from northern Thailand. We also combined 329 genotypes from previous studies of northern Thai populations bringing to a tota...

Research paper thumbnail of Genetic and linguistic correlation of the Kra-Dai-speaking groups in Thailand

Journal of human genetics, Jan 2, 2015

The Kra-Dai linguistic family includes Thai and Lao as well as a great number of languages spoken... more The Kra-Dai linguistic family includes Thai and Lao as well as a great number of languages spoken by ethnic minorities in Southeast Asia. In Thailand, a dozen of other Kra-Dai languages are spoken in addition to Thai, the national language. The genetic structure of the Kra-Dai-speaking populations in Thailand has been studied extensively using uniparentally inherited markers. To extend this line of genetic investigation, this study used 15 autosomal microsatellites of 500 individuals from 11 populations, belonging to nine Kra-Dai ethnicities, namely, the Kaleung, Phu Thai, Saek, Nyo, Lao Isan, Yuan, Black Tai, Phuan and Lue. These ethnolinguistic groups are dispersed in three different geographic regions of Thailand, that is, Northern, Northeastern and Central. The results show a very low average of pairwised Fst (0.0099), as well as no population substructure based on STRUCTURE analysis, indicating genetic homogeneity within the Kra-Dai-speaking group, possibly owing to shared ling...

Research paper thumbnail of Geography has more influence than language on maternal genetic structure of various northeastern Thai ethnicities

Journal of Human Genetics, 2014

Several literatures have shown the influence of geographic and linguistic factors in shaping gene... more Several literatures have shown the influence of geographic and linguistic factors in shaping genetic variation patterns, but their relative impact, if any, in the very heterogeneous northeastern region of Thailand has not yet been studied. This area, called Isan, is geographically structured in two wide basins, the Sakon Nakorn Basin and the Korat Basin, serving today as home to diverse ethnicities encompassing two different linguistic families, that is, the Austro-Asiatic; Suay (Kui), Mon, Chaobon (Nyahkur), So and Khmer, and the Tai-Kadai; Saek, Nyaw, Phu Tai, Kaleung and Lao Isan. In this study, we evaluated the relative role of geographic distance and barriers as well as linguistic differences as possible causes affecting the maternal genetic distances among northeastern Thai ethnicities. A 596-bp segment of the hypervariable region I mitochondrial DNA was utilized to elucidate the genetic structure and biological affinity from 433 individuals. Different statistical analyses agreed in suggesting that most ethnic groups in the Sakon Nakorn Basin are closely related. Mantel test revealed that genetic distances were highly associated to geographic (r ¼ 0.445, Po0.01) but not to linguistic (r ¼ 0.001, P40.01) distances. Three evolutionary models were compared by Approximate Bayesian Computation. The posterior probability of the scenario, which assumed an initial population divergence possibly related to reduced gene flow among basins, was equal or higher than 0.87. All analyses exhibited concordant results supporting that geography was the most relevant factor in determining the maternal genetic structure of northeastern Thai populations.

Research paper thumbnail of Genetic structure of the ethnic Lao groups from mainland Southeast Asia revealed by forensic microsatellites

Annals of Human Genetics

Laotians and Lao Isan are widely spread Lao groups who live in Laos and northeastern Thailand, re... more Laotians and Lao Isan are widely spread Lao groups who live in Laos and northeastern Thailand, respectively. We explored the genetic structure between them and other ethnic groups from Thailand to clarify historical patterns of admixture between Tai‐Kadai and Austroasiatic speakers, and to expand the forensic reference database for the region.

Research paper thumbnail of Forensic and genetic characterizations of diverse southern Thai populations based on 15 autosomal STRs

Scientific Reports

Southern Thailand is home to various populations; the Moklen, Moken and Urak Lawoi’ sea nomads an... more Southern Thailand is home to various populations; the Moklen, Moken and Urak Lawoi’ sea nomads and Maniq negrito are the minority, while the southern Thai groups (Buddhist and Muslim) are the majority. Although previous studies have generated forensic STR dataset for major groups, such data of the southern Thai minority have not been included; here we generated a regional forensic database of southern Thailand. We newly genotyped common 15 autosomal STRs in 184 unrelated southern Thais, including all minorities and majorities. When combined with previously published data of major southern Thais, this provides a total of 334 southern Thai samples. The forensic parameter results show appropriate values for personal identification and paternity testing; the probability of excluding paternity is 0.99999622, and the combined discrimination power is 0.999999999999999. Probably driven by genetic drift and/or isolation with small census size, we found genetic distinction of the Maniq and se...

Research paper thumbnail of Mitochondrial DNA-HVR1 Variation Reveals Genetic Heterogeneity in Thai-Isan Peoples from the Lower Region of

Copyright © 2014 Wibhu Kutanan et al. This is an open access article distributed under the Creati... more Copyright © 2014 Wibhu Kutanan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In accordance of the Creative Commons Attribution License all Copyrights © 2014 are reserved for SCIRP and the owner of the intellectual property Wibhu Kutanan et al. All Copyright © 2014 are guarded by law and by SCIRP as a guardian. The Thai-Isan are the largest ethnic group in Northeastern Thailand, who migrated from the territory of present-day Laos around 200 years ago. There are a limited number of previous studies of mtDNA diver-sity in Northeastern Thai populations. The present study investigates genetic variation and genetic struc-ture in three Thai-Isan populations by analyzing mitochondrial DNA variation in hypervariable region 1. We also evaluate genetic relationship and admixture between Thai-Isan and surrounding populati...

Research paper thumbnail of Autosomal Microsatellite Investigation Reveals Multiple Genetic Components of the Highlanders from Thailand

Genes, 2021

The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiati... more The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiatic-speaking (AA) Khmu, Htin and Lawa; the Hmong-Mien-speaking (HM) IuMien and Hmong; and the Sino-Tibetan-speaking (ST) Akha, Karen, Lahu and Lisu. Except the Lawa, the rest of the hill tribes migrated into their present habitats only very recently. The Thai hill tribes were of much interest to research groups focusing on study of cultural and genetic variation because of their unique languages and cultures. So far, there have been several genetic studies of the Thai hill tribes. However, complete forensic microsatellite database of the Thai hill tribes is still lacking. To construct such database, we newly generated 654 genotypes of 15 microsatellites commonly used in forensic investigation that belong to all the nine hill tribes and also non-hill tribe highlanders from northern Thailand. We also combined 329 genotypes from previous studies of northern Thai populations bringing to a tota...

Research paper thumbnail of Close genetic relationship between central Thai and Mon people in Thailand revealed by autosomal microsatellites

International Journal of Legal Medicine, 2020

Central Thailand is home to diverse populations with the central Thai constituting the major grou... more Central Thailand is home to diverse populations with the central Thai constituting the major group, while the Mon, who migrated from southern Myanmar, are sparsely distributed within the region. A total of 338 individuals of eight central Thai (246 samples) and three Mon populations (92 samples) were newly genotyped. When combined with our previously published Mon data, this provides a total of 139 Mon samples. We found genetic similarity between the central Thai and Mon and weak sub-structuring among Thais from central, northern, and northeastern Thailand. The forensic parameter results show high discrimination values which are appropriate for forensic personal identification and paternity testing in both the central Thai and Mon; the probabilities of excluding paternity are 0.999999112 and 0.999999031, respectively, and the combined discrimination power is 0.9999999999999999999999 in both groups. This regional allelic frequency on forensic microsatellites may serve as a useful ref...

Research paper thumbnail of Genetic Divergence of Austroasiatic Speaking Groups in the Northeast of Thailand: A Case Study on Northern Khmer and Kuy

Research paper thumbnail of Reconstructing the human genetic history of mainland Southeast Asia: insights from genome-wide data from Thailand and Laos

Thailand and Laos, located in the center of Mainland Southeast Asia (MSEA), harbor diverse ethnol... more Thailand and Laos, located in the center of Mainland Southeast Asia (MSEA), harbor diverse ethnolinguistic groups encompassing all five language families of MSEA: Tai-Kadai (TK), Austroasiatic (AA), Sino-Tibetan (ST), Hmong-Mien (HM) and Austronesian (AN). Previous genetic studies of Thai/Lao populations have focused almost exclusively on uniparental markers and there is a paucity of genome-wide studies. We therefore generated genome-wide SNP data for 33 ethnolinguistic groups, belonging to the five MSEA language families from Thailand and Laos, and analysed these together with data from modern Asian populations and SEA ancient samples. Overall, we find genetic structure according to language family, albeit with heterogeneity in the AA-, HM- and ST-speaking groups, and in the hill tribes, that reflects both population interactions and genetic drift. For the TK speaking groups, we find localized genetic structure that is driven by different levels of interaction with other groups in ...

Research paper thumbnail of Cultural variation impacts paternal and maternal genetic lineages of the Hmong-Mien and Sino-Tibetan groups from Thailand

European Journal of Human Genetics

The Hmong-Mien (HM) and Sino-Tibetan (ST) speaking groups are known as hill tribes in Thailand; t... more The Hmong-Mien (HM) and Sino-Tibetan (ST) speaking groups are known as hill tribes in Thailand; they were the subject of the first studies to show an impact of patrilocality vs. matrilocality on patterns of mitochondrial (mt) DNA vs. male-specific portion of the Y chromosome (MSY) variation. However, HM and ST groups have not been studied in as much detail as other Thai groups; here we report and analyze 234 partial MSY sequences (∼2.3 mB) and 416 complete mtDNA sequences from 14 populations that, when combined with our previous published data, provides the largest dataset yet for the hill tribes. We find a striking difference between Hmong and IuMien (Mien-speaking) groups: the Hmong are genetically different from both the IuMien and all other Thai groups, whereas the IuMien are genetically more similar to other linguistic groups than to the Hmong. In general, we find less of an impact of patrilocality vs. matrilocality on patterns of mtDNA vs. MSY variation than previous studies. ...

Research paper thumbnail of Genetic Diversity of the Endangered Mekong Giant Catfish, Striped Catfish, and Their Hybrids From Thailand

Tropical Conservation Science, 2019

Previous genetic investigations of the Mekong giant catfish (Pangasianodon gigas Chevey, 1913) an... more Previous genetic investigations of the Mekong giant catfish (Pangasianodon gigas Chevey, 1913) and striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) provided discordant results. Here, we sequenced mitochondrial (mt) DNA of the cytochrome B region, and a control region, to characterize the genetic variation of P. gigas, P. hypophthalmus, and hybrids of these two species. Among the three groups, P. hypophthalmus had the greatest diversity in both regions, yet all three studied groups showed lower genetic diversity compared to the results of previous studies. The Bayesian Skyline Plots showed a reduction in effective population sizes of the parental species. The hybrids were found to have a constant population size with a recent divergence time. As expected, the network and neighbor joining tree showed a close maternal genetic relationship between the hybrid and P. hypophthalmus, a reflection of the breeding between male P. gigas and female P. hypophthalmus. Our results provide genetic information on these endangered fish that will be useful for both conservation and commercial breeding programs.

Research paper thumbnail of Genetic Structure and Forensic Utility of 23 Autosomal STRs of the Ethnic Lao Groups From Laos and Thailand

Frontiers in Genetics

The Lao Isan and Laotian are the major groups in the area of present-day northeastern Thailand an... more The Lao Isan and Laotian are the major groups in the area of present-day northeastern Thailand and Laos, respectively. Several previous genetic and forensic studies indicated an admixed genetic structure of Lao Isan with the local Austroasiatic speaking groups, e.g. Khmer, whereas there is a paucity of reporting Laotian’s forensic short tandem repeats (STRs). Here, we newly generated 451 genotypes of seven Lao Isan and three Laotian populations (two Lao Lum and one Lao Thoeng) using 23 autosomal STRs embedded in VerifilerTM plus PCR Amplification kit. We reported allelic frequency and forensic parameters in different dataset: combined ethnic Lao groups, combined Lao Isan populations and combined Laotians. Overall, the forensic parameter results indicate that this set of STRs is suitable for forensic investigation. The anthropological results revealed the genetic homogeneity of Tai-Kadai speaking Lao groups from Thailand and Laos, consistent with previous studies, while the Austroasi...

Research paper thumbnail of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

BMC Plant Biology

Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season... more Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season grasses. Erianthus species are widely distributed throughout Southeast Asia, East Asia and South Asia. Erianthus arundinaceus (Retz.) Jeswiet is highly adaptable to the environment, has a high percentage of dry matter, and is highly productive. Recently, this species has attracted attention as a novel bioenergy crop and as a breeding material for sugarcane improvement. Such interest in E. arundinaceus has accelerated the collection and conservation of its genetic resources, mainly in Asian countries, and also evaluation of morphological, agricultural, and cytogenetic features in germplasm collections. In Thailand, genetic resources of E. arundinaceus have been collected over the past 20 years and their phenotypic traits have been evaluated. However, the genetic differences and relatedness of the germplasms are not fully understood. Results A set of 41 primer pairs for nuclear simple seq...

Research paper thumbnail of Additional file 6 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 6: Figure S1. Geographic locations of 121 Erianthus accessions collected in Thail... more Additional file 6: Figure S1. Geographic locations of 121 Erianthus accessions collected in Thailand, and a pie chart of the populations and a bar chart of ancestry proportion in the 7 admixtures. Colors correspond to those in Fig. 1 at K = 4. Admixture group is indicated in gray. Accession numbers are listed as map No. in Table S2.

Research paper thumbnail of Additional file 5 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 5: Table S5. Sequences of primers used for amplification and sequencing of chloro... more Additional file 5: Table S5. Sequences of primers used for amplification and sequencing of chloroplast DNA regions in Thai Erianthus accessions.

Research paper thumbnail of Additional file 3 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 3: Table S3. GenBank accession numbers of three non-coding regions of chloroplast... more Additional file 3: Table S3. GenBank accession numbers of three non-coding regions of chloroplast DNA and their concatenated sequences in 121 Thai Erianthus accessions.

Research paper thumbnail of Additional file 4 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 4: Table S4. Statistical analysis of haplotype diversity, nucleotide diversity, a... more Additional file 4: Table S4. Statistical analysis of haplotype diversity, nucleotide diversity, and neutrality in each chloroplast DNA region in 121 Thai Erianthus accessions.

Research paper thumbnail of Additional file 2 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 2: Table S2. Sampling locations of 121 Erianthus accessions collected in Thailand... more Additional file 2: Table S2. Sampling locations of 121 Erianthus accessions collected in Thailand, their grouping by each analysis, and size of amplified fragment in each accession.

Research paper thumbnail of Additional file 1 of Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

Additional file 1: Table S1. Characteristics of 28 SSR loci in 121 Thai Erianthus accessions.

Research paper thumbnail of Autosomal Microsatellite Investigation Reveals Multiple Genetic Components of the Highlanders from Thailand

Genes, 2021

The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiati... more The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiatic-speaking (AA) Khmu, Htin and Lawa; the Hmong-Mien-speaking (HM) IuMien and Hmong; and the Sino-Tibetan-speaking (ST) Akha, Karen, Lahu and Lisu. Except the Lawa, the rest of the hill tribes migrated into their present habitats only very recently. The Thai hill tribes were of much interest to research groups focusing on study of cultural and genetic variation because of their unique languages and cultures. So far, there have been several genetic studies of the Thai hill tribes. However, complete forensic microsatellite database of the Thai hill tribes is still lacking. To construct such database, we newly generated 654 genotypes of 15 microsatellites commonly used in forensic investigation that belong to all the nine hill tribes and also non-hill tribe highlanders from northern Thailand. We also combined 329 genotypes from previous studies of northern Thai populations bringing to a tota...

Research paper thumbnail of Genetic and linguistic correlation of the Kra-Dai-speaking groups in Thailand

Journal of human genetics, Jan 2, 2015

The Kra-Dai linguistic family includes Thai and Lao as well as a great number of languages spoken... more The Kra-Dai linguistic family includes Thai and Lao as well as a great number of languages spoken by ethnic minorities in Southeast Asia. In Thailand, a dozen of other Kra-Dai languages are spoken in addition to Thai, the national language. The genetic structure of the Kra-Dai-speaking populations in Thailand has been studied extensively using uniparentally inherited markers. To extend this line of genetic investigation, this study used 15 autosomal microsatellites of 500 individuals from 11 populations, belonging to nine Kra-Dai ethnicities, namely, the Kaleung, Phu Thai, Saek, Nyo, Lao Isan, Yuan, Black Tai, Phuan and Lue. These ethnolinguistic groups are dispersed in three different geographic regions of Thailand, that is, Northern, Northeastern and Central. The results show a very low average of pairwised Fst (0.0099), as well as no population substructure based on STRUCTURE analysis, indicating genetic homogeneity within the Kra-Dai-speaking group, possibly owing to shared ling...

Research paper thumbnail of Geography has more influence than language on maternal genetic structure of various northeastern Thai ethnicities

Journal of Human Genetics, 2014

Several literatures have shown the influence of geographic and linguistic factors in shaping gene... more Several literatures have shown the influence of geographic and linguistic factors in shaping genetic variation patterns, but their relative impact, if any, in the very heterogeneous northeastern region of Thailand has not yet been studied. This area, called Isan, is geographically structured in two wide basins, the Sakon Nakorn Basin and the Korat Basin, serving today as home to diverse ethnicities encompassing two different linguistic families, that is, the Austro-Asiatic; Suay (Kui), Mon, Chaobon (Nyahkur), So and Khmer, and the Tai-Kadai; Saek, Nyaw, Phu Tai, Kaleung and Lao Isan. In this study, we evaluated the relative role of geographic distance and barriers as well as linguistic differences as possible causes affecting the maternal genetic distances among northeastern Thai ethnicities. A 596-bp segment of the hypervariable region I mitochondrial DNA was utilized to elucidate the genetic structure and biological affinity from 433 individuals. Different statistical analyses agreed in suggesting that most ethnic groups in the Sakon Nakorn Basin are closely related. Mantel test revealed that genetic distances were highly associated to geographic (r ¼ 0.445, Po0.01) but not to linguistic (r ¼ 0.001, P40.01) distances. Three evolutionary models were compared by Approximate Bayesian Computation. The posterior probability of the scenario, which assumed an initial population divergence possibly related to reduced gene flow among basins, was equal or higher than 0.87. All analyses exhibited concordant results supporting that geography was the most relevant factor in determining the maternal genetic structure of northeastern Thai populations.

Research paper thumbnail of Genetic structure of the ethnic Lao groups from mainland Southeast Asia revealed by forensic microsatellites

Annals of Human Genetics

Laotians and Lao Isan are widely spread Lao groups who live in Laos and northeastern Thailand, re... more Laotians and Lao Isan are widely spread Lao groups who live in Laos and northeastern Thailand, respectively. We explored the genetic structure between them and other ethnic groups from Thailand to clarify historical patterns of admixture between Tai‐Kadai and Austroasiatic speakers, and to expand the forensic reference database for the region.

Research paper thumbnail of Forensic and genetic characterizations of diverse southern Thai populations based on 15 autosomal STRs

Scientific Reports

Southern Thailand is home to various populations; the Moklen, Moken and Urak Lawoi’ sea nomads an... more Southern Thailand is home to various populations; the Moklen, Moken and Urak Lawoi’ sea nomads and Maniq negrito are the minority, while the southern Thai groups (Buddhist and Muslim) are the majority. Although previous studies have generated forensic STR dataset for major groups, such data of the southern Thai minority have not been included; here we generated a regional forensic database of southern Thailand. We newly genotyped common 15 autosomal STRs in 184 unrelated southern Thais, including all minorities and majorities. When combined with previously published data of major southern Thais, this provides a total of 334 southern Thai samples. The forensic parameter results show appropriate values for personal identification and paternity testing; the probability of excluding paternity is 0.99999622, and the combined discrimination power is 0.999999999999999. Probably driven by genetic drift and/or isolation with small census size, we found genetic distinction of the Maniq and se...

Research paper thumbnail of Mitochondrial DNA-HVR1 Variation Reveals Genetic Heterogeneity in Thai-Isan Peoples from the Lower Region of

Copyright © 2014 Wibhu Kutanan et al. This is an open access article distributed under the Creati... more Copyright © 2014 Wibhu Kutanan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In accordance of the Creative Commons Attribution License all Copyrights © 2014 are reserved for SCIRP and the owner of the intellectual property Wibhu Kutanan et al. All Copyright © 2014 are guarded by law and by SCIRP as a guardian. The Thai-Isan are the largest ethnic group in Northeastern Thailand, who migrated from the territory of present-day Laos around 200 years ago. There are a limited number of previous studies of mtDNA diver-sity in Northeastern Thai populations. The present study investigates genetic variation and genetic struc-ture in three Thai-Isan populations by analyzing mitochondrial DNA variation in hypervariable region 1. We also evaluate genetic relationship and admixture between Thai-Isan and surrounding populati...

Research paper thumbnail of Autosomal Microsatellite Investigation Reveals Multiple Genetic Components of the Highlanders from Thailand

Genes, 2021

The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiati... more The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiatic-speaking (AA) Khmu, Htin and Lawa; the Hmong-Mien-speaking (HM) IuMien and Hmong; and the Sino-Tibetan-speaking (ST) Akha, Karen, Lahu and Lisu. Except the Lawa, the rest of the hill tribes migrated into their present habitats only very recently. The Thai hill tribes were of much interest to research groups focusing on study of cultural and genetic variation because of their unique languages and cultures. So far, there have been several genetic studies of the Thai hill tribes. However, complete forensic microsatellite database of the Thai hill tribes is still lacking. To construct such database, we newly generated 654 genotypes of 15 microsatellites commonly used in forensic investigation that belong to all the nine hill tribes and also non-hill tribe highlanders from northern Thailand. We also combined 329 genotypes from previous studies of northern Thai populations bringing to a tota...

Research paper thumbnail of Close genetic relationship between central Thai and Mon people in Thailand revealed by autosomal microsatellites

International Journal of Legal Medicine, 2020

Central Thailand is home to diverse populations with the central Thai constituting the major grou... more Central Thailand is home to diverse populations with the central Thai constituting the major group, while the Mon, who migrated from southern Myanmar, are sparsely distributed within the region. A total of 338 individuals of eight central Thai (246 samples) and three Mon populations (92 samples) were newly genotyped. When combined with our previously published Mon data, this provides a total of 139 Mon samples. We found genetic similarity between the central Thai and Mon and weak sub-structuring among Thais from central, northern, and northeastern Thailand. The forensic parameter results show high discrimination values which are appropriate for forensic personal identification and paternity testing in both the central Thai and Mon; the probabilities of excluding paternity are 0.999999112 and 0.999999031, respectively, and the combined discrimination power is 0.9999999999999999999999 in both groups. This regional allelic frequency on forensic microsatellites may serve as a useful ref...

Research paper thumbnail of Genetic Divergence of Austroasiatic Speaking Groups in the Northeast of Thailand: A Case Study on Northern Khmer and Kuy

Research paper thumbnail of Reconstructing the human genetic history of mainland Southeast Asia: insights from genome-wide data from Thailand and Laos

Thailand and Laos, located in the center of Mainland Southeast Asia (MSEA), harbor diverse ethnol... more Thailand and Laos, located in the center of Mainland Southeast Asia (MSEA), harbor diverse ethnolinguistic groups encompassing all five language families of MSEA: Tai-Kadai (TK), Austroasiatic (AA), Sino-Tibetan (ST), Hmong-Mien (HM) and Austronesian (AN). Previous genetic studies of Thai/Lao populations have focused almost exclusively on uniparental markers and there is a paucity of genome-wide studies. We therefore generated genome-wide SNP data for 33 ethnolinguistic groups, belonging to the five MSEA language families from Thailand and Laos, and analysed these together with data from modern Asian populations and SEA ancient samples. Overall, we find genetic structure according to language family, albeit with heterogeneity in the AA-, HM- and ST-speaking groups, and in the hill tribes, that reflects both population interactions and genetic drift. For the TK speaking groups, we find localized genetic structure that is driven by different levels of interaction with other groups in ...

Research paper thumbnail of Cultural variation impacts paternal and maternal genetic lineages of the Hmong-Mien and Sino-Tibetan groups from Thailand

European Journal of Human Genetics

The Hmong-Mien (HM) and Sino-Tibetan (ST) speaking groups are known as hill tribes in Thailand; t... more The Hmong-Mien (HM) and Sino-Tibetan (ST) speaking groups are known as hill tribes in Thailand; they were the subject of the first studies to show an impact of patrilocality vs. matrilocality on patterns of mitochondrial (mt) DNA vs. male-specific portion of the Y chromosome (MSY) variation. However, HM and ST groups have not been studied in as much detail as other Thai groups; here we report and analyze 234 partial MSY sequences (∼2.3 mB) and 416 complete mtDNA sequences from 14 populations that, when combined with our previous published data, provides the largest dataset yet for the hill tribes. We find a striking difference between Hmong and IuMien (Mien-speaking) groups: the Hmong are genetically different from both the IuMien and all other Thai groups, whereas the IuMien are genetically more similar to other linguistic groups than to the Hmong. In general, we find less of an impact of patrilocality vs. matrilocality on patterns of mtDNA vs. MSY variation than previous studies. ...

Research paper thumbnail of Genetic Diversity of the Endangered Mekong Giant Catfish, Striped Catfish, and Their Hybrids From Thailand

Tropical Conservation Science, 2019

Previous genetic investigations of the Mekong giant catfish (Pangasianodon gigas Chevey, 1913) an... more Previous genetic investigations of the Mekong giant catfish (Pangasianodon gigas Chevey, 1913) and striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) provided discordant results. Here, we sequenced mitochondrial (mt) DNA of the cytochrome B region, and a control region, to characterize the genetic variation of P. gigas, P. hypophthalmus, and hybrids of these two species. Among the three groups, P. hypophthalmus had the greatest diversity in both regions, yet all three studied groups showed lower genetic diversity compared to the results of previous studies. The Bayesian Skyline Plots showed a reduction in effective population sizes of the parental species. The hybrids were found to have a constant population size with a recent divergence time. As expected, the network and neighbor joining tree showed a close maternal genetic relationship between the hybrid and P. hypophthalmus, a reflection of the breeding between male P. gigas and female P. hypophthalmus. Our results provide genetic information on these endangered fish that will be useful for both conservation and commercial breeding programs.