S. Sukno - Academia.edu (original) (raw)

Papers by S. Sukno

Genome biology, 2011

Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevanc... more Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevance when the prey is a plant pathogen, providing a strategy for biological control of pests for plant protection. Probably, the most studied biocontrol agents are species of the genus Hypocrea/Trichoderma.

Applied and Environmental Microbiology, 2007

Colletotrichum graminicola is a filamentous ascomycete that causes anthracnose disease of maize. ... more Colletotrichum graminicola is a filamentous ascomycete that causes anthracnose disease of maize. While the fungus can cause devastating foliar leaf blight and stalk rot diseases, little is known about its ability to infect roots. Previously published reports suggest that C. graminicola may infect maize roots and that root infections may contribute to the colonization of aboveground plant tissues, leading to disease. To determine whether C. graminicola can infect maize roots and whether root infections can result in the colonization of aboveground plant tissues, we developed a green fluorescent protein-tagged strain and used it to study the plant root colonization and infection process in vivo. We observed structures produced by other root pathogenic fungi, including runner hyphae, hyphopodia, and microsclerotia. A mosaic pattern of infection resulted from specific epidermal and cortical cells becoming infected by intercellular hyphae while surrounding cells were uninfected, a patter...

Colletotrichum sublineola is a filamentous fungus that causes anthracnose disease on sorghum. We ... more Colletotrichum sublineola is a filamentous fungus that causes anthracnose disease on sorghum. We report a draft whole-genome shotgun sequence and gene annotation of the nuclear genome of this fungus using Illumina sequencing.

Molecular Plant Pathology, 2016

PloS one, 2015

Fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging t... more Fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging to Rosaceae family. Colletotrichum acutatum sensu lato (s.l.) is considered to be the second most economically important pathogen worldwide affecting strawberries. A collection of 148 Colletotrichum spp. isolates including 67 C. acutatum s.l. isolates associated with the phytosanitary history of UK strawberry production were used to characterize multi-locus genetic variation of this pathogen in the UK, relative to additional reference isolates that represent a worldwide sampling of the diversity of the fungus. The evidence indicates that three different species C. nymphaeae, C. godetiae and C. fioriniae are associated with strawberry production in the UK, which correspond to previously designated genetic groups A2, A4 and A3, respectively. Among these species, 12 distinct haplotypes were identified suggesting multiple introductions into the country. A subset of isolates was also used to c...

Journal of nematology, 2006

The root-knot nematode Meloidogyne incognita is an obligate endoparasite of plant roots and stimu... more The root-knot nematode Meloidogyne incognita is an obligate endoparasite of plant roots and stimulates elaborate modifications of selected root vascular cells to form giant cells for feeding. An Arabidopsis thaliana endoglucanase (Atcel1) promoter is activated in giant cells that were formed in Atcel1::UidA transgenic tobacco and Arabidopsis plants. Activity of the full-length Atcel1 promoter was detected in root and shoot elongation zones and in the lateral root primordia. Different 5' and internal deletions of regions of the 1,673 bp Atcel1 promoter were each fused to the UidA reporter gene and transformed in tobacco, and roots of the transformants were inoculated with M. incognita to assay for GUS expression in giant cells and noninfected plant tissues. Comparison of the Atcel1 promoter deletion constructs showed that the region between -1,673 and -1,171 (fragment 1) was essential for Atcel1 promoter activity in giant cells and roots. Fragment 1 alone, however, was not suffic...

Nitric Oxide in Plant Physiology

In addition to its economic impact, Colletotrichum acutatum sensu lato is an interesting model fo... more In addition to its economic impact, Colletotrichum acutatum sensu lato is an interesting model for molecular investigations due to the diversity of host-determined specialization and reproductive lifestyles within the species complex. The pathogen Colletotrichum fioriniae forms part of this species complex and causes anthracnose in a wide range of crops and wild plants worldwide. Some members of this species have also been reported to be entomopathogenic. Here, we report the draft genome sequence of a heterothallic reference isolate of C. fioriniae (strain PJ7). This sequence provides a range of new resources that serve as a useful platform for further research in the field.

Molecular Plant-Microbe Interactions®, 2013

Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. T... more Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. The transcription factor pacC is a regulator of pH-controlled genes and is essential for successful colonization. We present here the sequence assembly of the Colletotrichum fruit pathogen and use it to explore the global regulation of pathogenicity by ambient pH. The assembled genome size was 54 Mb, encoding 18,456 genes. Transcriptomes of the wild type and ΔpacC mutant were established by RNA-seq and explored for their global pH-dependent gene regulation. The analysis showed that pacC upregulates 478 genes and downregulates 483 genes, comprising 5% of the fungal genome, including transporters, antioxidants, and cell-wall-degrading enzymes. Interestingly, gene families with similar functionality are both up- and downregulated by pacC. Global analysis of secreted genes showed significant pacC activation of degradative enzymes at alkaline pH and during fruit infection. Select genes from al...

Molecular Plant Pathology, 2004

1986., 1986

Autor: Díaz Zorita, Martín; Duarte, Gustavo A.; Aguirrezábal, Luis A.; Andrade, Fernando H.; Arag... more Autor: Díaz Zorita, Martín; Duarte, Gustavo A.; Aguirrezábal, Luis A.; Andrade, Fernando H.; Aragón, Jorge R.; Avila, Federico; Bartosik, Ricardo E.; Baumer, Ricardo; Bragachini, Mario A.; Di Napoli, Marcelo; Dosio, Guillermo A.; Duarte, Gustavo A.; Fernández Canigia, María ...

Molecular Plant Pathology, 2004

The promoter of the Arabidopsis thaliana Cel1 endo-1,4-β β β β glucanase gene is differentially e... more The promoter of the Arabidopsis thaliana Cel1 endo-1,4-β β β β glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes

Genome biology, 2011

Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevanc... more Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevance when the prey is a plant pathogen, providing a strategy for biological control of pests for plant protection. Probably, the most studied biocontrol agents are species of the genus Hypocrea/Trichoderma.

Applied and Environmental Microbiology, 2007

Colletotrichum graminicola is a filamentous ascomycete that causes anthracnose disease of maize. ... more Colletotrichum graminicola is a filamentous ascomycete that causes anthracnose disease of maize. While the fungus can cause devastating foliar leaf blight and stalk rot diseases, little is known about its ability to infect roots. Previously published reports suggest that C. graminicola may infect maize roots and that root infections may contribute to the colonization of aboveground plant tissues, leading to disease. To determine whether C. graminicola can infect maize roots and whether root infections can result in the colonization of aboveground plant tissues, we developed a green fluorescent protein-tagged strain and used it to study the plant root colonization and infection process in vivo. We observed structures produced by other root pathogenic fungi, including runner hyphae, hyphopodia, and microsclerotia. A mosaic pattern of infection resulted from specific epidermal and cortical cells becoming infected by intercellular hyphae while surrounding cells were uninfected, a patter...

Colletotrichum sublineola is a filamentous fungus that causes anthracnose disease on sorghum. We ... more Colletotrichum sublineola is a filamentous fungus that causes anthracnose disease on sorghum. We report a draft whole-genome shotgun sequence and gene annotation of the nuclear genome of this fungus using Illumina sequencing.

Molecular Plant Pathology, 2016

PloS one, 2015

Fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging t... more Fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging to Rosaceae family. Colletotrichum acutatum sensu lato (s.l.) is considered to be the second most economically important pathogen worldwide affecting strawberries. A collection of 148 Colletotrichum spp. isolates including 67 C. acutatum s.l. isolates associated with the phytosanitary history of UK strawberry production were used to characterize multi-locus genetic variation of this pathogen in the UK, relative to additional reference isolates that represent a worldwide sampling of the diversity of the fungus. The evidence indicates that three different species C. nymphaeae, C. godetiae and C. fioriniae are associated with strawberry production in the UK, which correspond to previously designated genetic groups A2, A4 and A3, respectively. Among these species, 12 distinct haplotypes were identified suggesting multiple introductions into the country. A subset of isolates was also used to c...

Journal of nematology, 2006

The root-knot nematode Meloidogyne incognita is an obligate endoparasite of plant roots and stimu... more The root-knot nematode Meloidogyne incognita is an obligate endoparasite of plant roots and stimulates elaborate modifications of selected root vascular cells to form giant cells for feeding. An Arabidopsis thaliana endoglucanase (Atcel1) promoter is activated in giant cells that were formed in Atcel1::UidA transgenic tobacco and Arabidopsis plants. Activity of the full-length Atcel1 promoter was detected in root and shoot elongation zones and in the lateral root primordia. Different 5' and internal deletions of regions of the 1,673 bp Atcel1 promoter were each fused to the UidA reporter gene and transformed in tobacco, and roots of the transformants were inoculated with M. incognita to assay for GUS expression in giant cells and noninfected plant tissues. Comparison of the Atcel1 promoter deletion constructs showed that the region between -1,673 and -1,171 (fragment 1) was essential for Atcel1 promoter activity in giant cells and roots. Fragment 1 alone, however, was not suffic...

Nitric Oxide in Plant Physiology

In addition to its economic impact, Colletotrichum acutatum sensu lato is an interesting model fo... more In addition to its economic impact, Colletotrichum acutatum sensu lato is an interesting model for molecular investigations due to the diversity of host-determined specialization and reproductive lifestyles within the species complex. The pathogen Colletotrichum fioriniae forms part of this species complex and causes anthracnose in a wide range of crops and wild plants worldwide. Some members of this species have also been reported to be entomopathogenic. Here, we report the draft genome sequence of a heterothallic reference isolate of C. fioriniae (strain PJ7). This sequence provides a range of new resources that serve as a useful platform for further research in the field.

Molecular Plant-Microbe Interactions®, 2013

Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. T... more Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. The transcription factor pacC is a regulator of pH-controlled genes and is essential for successful colonization. We present here the sequence assembly of the Colletotrichum fruit pathogen and use it to explore the global regulation of pathogenicity by ambient pH. The assembled genome size was 54 Mb, encoding 18,456 genes. Transcriptomes of the wild type and ΔpacC mutant were established by RNA-seq and explored for their global pH-dependent gene regulation. The analysis showed that pacC upregulates 478 genes and downregulates 483 genes, comprising 5% of the fungal genome, including transporters, antioxidants, and cell-wall-degrading enzymes. Interestingly, gene families with similar functionality are both up- and downregulated by pacC. Global analysis of secreted genes showed significant pacC activation of degradative enzymes at alkaline pH and during fruit infection. Select genes from al...

Molecular Plant Pathology, 2004

1986., 1986

Autor: Díaz Zorita, Martín; Duarte, Gustavo A.; Aguirrezábal, Luis A.; Andrade, Fernando H.; Arag... more Autor: Díaz Zorita, Martín; Duarte, Gustavo A.; Aguirrezábal, Luis A.; Andrade, Fernando H.; Aragón, Jorge R.; Avila, Federico; Bartosik, Ricardo E.; Baumer, Ricardo; Bragachini, Mario A.; Di Napoli, Marcelo; Dosio, Guillermo A.; Duarte, Gustavo A.; Fernández Canigia, María ...

Molecular Plant Pathology, 2004

The promoter of the Arabidopsis thaliana Cel1 endo-1,4-β β β β glucanase gene is differentially e... more The promoter of the Arabidopsis thaliana Cel1 endo-1,4-β β β β glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes