Sabrine Kobbi - Academia.edu (original) (raw)

Papers by Sabrine Kobbi

Research paper thumbnail of Peptide from RuBisCO protein hydrolysate and its application in beef meat preservation

Pesquisa Agropecuaria Brasileira, Dec 31, 2022

Research paper thumbnail of Physicochemical and Biological Properties of Eel By-Products Protein Hydrolysates: Potential Application to Meat Product Preservation

Waste and Biomass Valorization, Aug 13, 2018

European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides wit... more European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides with antibacterial and antioxidant activity. Protein hydrolysates from Anguilla by-27 products obtained by treatment with Savinase® (PHAB-s) have high protein content than that 28 produced with Protamex ® (PHAB-p). Furthemore, both protein hydrolysates had a high 29 percentage of essential amino acids. In addition, results indicated that PHAB-s and PHAB-p 30 exhibited different degrees of antioxidant activities, evaluated by several antioxidant assays. 31 Thus, PHAB-s displayed high antioxidant activity, in terms of DPPH-radical scavenging effect 32 (IC50=5.2 mg/ml), β-carotene bleaching inhibitory effect (CI50=4.4 mg/ml) and metal chelating 33 activity. At 10 mg/ml, PHAB-p showed the highest reducing power. Antibacterial activities of 34 PHAB-s and PHAB-p were also evaluated. PHAB-s showed the most important inhibitory effects 35 against seven bacterial species. The application of PHAB-s and PHAB-p on minced meat as 36 preservative reduced the lipid oxidation and inhibited the microbial growth during chilled storage 37 for eleven days. 38 Statement of Novelty 39 Fish processing wastes are one of the important sources of natural bioactive compounds. 40 The recovery of these valuable components would not only improve the economy for fish 41 processors, but also would minimize the pollution potential of the fish processing waste. 42 Recently, our research group has been looking for new bioactive compounds from marine by-43 products. Further exploration of protein hydrolysates and peptides in other marine species can 44 provide a wider insight into studies on their biological and functional proprieties. In this context, 45 the objective of this investigation was to study bioactive potential of protein hydrolysates from 46 European eel (Anguilla anguilla) by-products.

Research paper thumbnail of Synthesis and antibacterial activity of new peptides from Alfalfa RuBisCO protein hydrolysates and mode of action via a membrane damage mechanism against Listeria innocua

Microbial pathogenesis, Jan 6, 2017

In this work we evaluated the mode of action of six new synthesized peptides (Met-Asp-Asn; Glu-le... more In this work we evaluated the mode of action of six new synthesized peptides (Met-Asp-Asn; Glu-leu-Ala-Ala-Ala-Cys; Leu-Arg-Asp-Asp-Phe; Gly-Asn-Ala-Pro-Gly-Ala-Val-Ala; Ala-Leu-Arg-Met-Ser-Gly and Arg-Asp-Arg-Phe-Leu), previously identified, from the most active peptide fractions of RuBisCO peptic hydrolysate against Listeria innocua via a membrane damage mechanism. Antibacterial effect and the minimum inhibitory concentrations (MIC) of these peptides were evaluated against six strains and their hemolytic activities towards bovine erythrocytes were determined. Prediction of the secondary structure of peptides indicated that these new antibacterial peptides are characterized by a short peptide chains (3-8 amino acid) and a random coli structure. Moreover, it was observed that one key characteristic of antibacterial peptides is the presence of specific amino acids such as cysteine, glycine, arginine and aspartic acid. In addition the determination of the extracellular potassium conce...

Research paper thumbnail of Identification and molecular docking of novel ACE inhibitory peptides from protein hydrolysates of shrimp waste

Engineering in Life Sciences, 2018

The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive... more The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive activity of shrimp waste proteins was evaluated. The physicochemical characterization, interfacial tension, and surface characteristics of shrimp waste protein hydrolysates (SWPH) using different enzyme/substrate (E/S) (SWPH 5 (SWPH using E/S = 5), SWPH 15 (SWPH using E/S = 15), and SWPH 40 (SWPH using E/S = 40)) were also studied. SWPH 5 , SWPH 15 , and SWPH 40 had an isoelectric pH around 2.07, 2.17, and 2.54 respectively. SWPH 5 exhibited the lowest interfacial tension (68.96 mN/m) followed by SWPH 15 (69.36 mN/m) and SWPH 40 (70.29 mN/m). The in vitro ACE inhibitory activity of shrimp waste protein hydrolysates showed that the most active hydrolysate was obtained using an enzyme/substrate of 15 U/mg (SWPH 15). SWPH 15 had a lower IC 50 value (2.17 mg/mL) than that of SWPH 5 and SWPH 40 (3.65 and 5.7 mg/mL, respectively). This hydrolysate was then purified and characterized. Fraction F1 separated by Sephadex G25 column which presents the best ACE inhibition activity was then separated by reversed-phase high performance liquid chromatography. Four ACE inhibitory peptides were identified and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were SSSKAKKMP, HGEGGRSTHE, WLGHGGRPDHE, and WRMDIDGDIMISE-QEAHQR. The structural modeling of anti-ACE peptides from shrimp waste through docking simulations results showed that these peptides bound to ACE with high affinity.

Research paper thumbnail of Physicochemical and Biological Properties of Eel By-Products Protein Hydrolysates: Potential Application to Meat Product Preservation

Waste and Biomass Valorization, 2018

European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides wit... more European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides with antibacterial and antioxidant activity. Protein hydrolysates from Anguilla by-27 products obtained by treatment with Savinase® (PHAB-s) have high protein content than that 28 produced with Protamex ® (PHAB-p). Furthemore, both protein hydrolysates had a high 29 percentage of essential amino acids. In addition, results indicated that PHAB-s and PHAB-p 30 exhibited different degrees of antioxidant activities, evaluated by several antioxidant assays. 31 Thus, PHAB-s displayed high antioxidant activity, in terms of DPPH-radical scavenging effect 32 (IC50=5.2 mg/ml), β-carotene bleaching inhibitory effect (CI50=4.4 mg/ml) and metal chelating 33 activity. At 10 mg/ml, PHAB-p showed the highest reducing power. Antibacterial activities of 34 PHAB-s and PHAB-p were also evaluated. PHAB-s showed the most important inhibitory effects 35 against seven bacterial species. The application of PHAB-s and PHAB-p on minced meat as 36 preservative reduced the lipid oxidation and inhibited the microbial growth during chilled storage 37 for eleven days. 38 Statement of Novelty 39 Fish processing wastes are one of the important sources of natural bioactive compounds. 40 The recovery of these valuable components would not only improve the economy for fish 41 processors, but also would minimize the pollution potential of the fish processing waste. 42 Recently, our research group has been looking for new bioactive compounds from marine by-43 products. Further exploration of protein hydrolysates and peptides in other marine species can 44 provide a wider insight into studies on their biological and functional proprieties. In this context, 45 the objective of this investigation was to study bioactive potential of protein hydrolysates from 46 European eel (Anguilla anguilla) by-products.

Research paper thumbnail of Antibacterial activity of novel peptides isolated from protein hydrolysates of RuBisCO purified from green juice alfalfa

Journal of Functional Foods, 2015

The present paper reports on the isolation and characterization of novel bioactive peptides by th... more The present paper reports on the isolation and characterization of novel bioactive peptides by the hydrolysis of RuBisCO proteins. Antibacterial activity was evaluated against Gramnegative (Escherichia coli and Salmonella enterica) and Gram-positive (Listeria innocua, Staphylococcus aureus, Enterococcus faecalis, Micrococcus luteus and Bacillus subtilis) bacteria. A simple method, consisting in the precipitation of the active peptides under ionic strength and pH conditions, was used to separate antimicrobial peptides from complex peptic hydrolysates of RuBisCO obtained by treatment with Pepsin ® (DH = 18.8%). The obtained peptide extract was fractionated using RP-HPLC, and the active fractions were analyzed by liquid chromatography, electrospray ionization, and tandem mass spectrometry (LC-ESI-MS). Among a total of twelve identified peptides, three new pure antibacterial peptides, namely (Met-Asp-Asn), (Glu-Leu-Ala-Ala-Ala-Cys) and (Leu-Arg-Asp-Asp-Phe), were obtained under hydrolytic conditions. The latter peptides were highly active against the tested strains. The three peptides did not show hemolytic activity towards bovine erythrocytes. Taken together, the results suggest that the new peptides isolated from RuBisCO could offer potential ingredients for the functional foods industry.

Research paper thumbnail of Purification de la RuBisCO à partir de la Luzerne, hydrolyse enzymatique, identification, structure-fonction des peptides bioactifs et leur valorisation dans des produits alimentaires

La luzerne est la plante la plus cultivee dans le monde et est une excellente source de proteines... more La luzerne est la plante la plus cultivee dans le monde et est une excellente source de proteines. Cependant, la RuBisCO a montre le plus d'interet. Cette proteine a ete etiquetee comme la proteine la plus abondante sur terre, elle constitue environ 65% (p/p) des proteines solubles de la luzerne. Dans ce travail une nouvelle methode a ete mise en place pour la purification de la RuBisCO a partir de la poudre de luzerne 10% (p/v) en utilisant deux solvants differents et l’effet du pH. Premierement, des methodes d’analyse qualitative et quantitative ont ete utilisees pour confirmer l’efficacite de la methode de purification qui pourrait remplacer certains procedes industriels classiques. Puis, une hydrolyse pepsique a ete realisee sur la RuBisCO purifiee qui aboutit a une forte population peptidique bioactive. Les peptides finaux de 24h d’hydrolyse ont montre une meilleure activite antibacterienne ou antioxydante par rapport aux autres hydrolysats. 9 nouveaux peptides antibacterie...

Research paper thumbnail of Purification and Recovery of RuBisCO Protein from Alfalfa Green Juice: Antioxidative Properties of Generated Protein Hydrolysate

Waste and Biomass Valorization, 2016

RuBisCO is the main soluble protein of alfalfa juice. Despite its attractive applications in a wi... more RuBisCO is the main soluble protein of alfalfa juice. Despite its attractive applications in a wide range of food products this protein remains largely underutilized. Accordingly, the present study was undertaken to investigate the feasibility and efficiency of using ammonium hydroxide (NH 4 OH) and precipitation at pH 3 for purification and recovery of RuBisCO from industrial Alfalfa green juice. The performance of the proposed RuBisCO recovery method was evaluated through qualitative and quantitative analysis. The results obtained by the proposed method were compared to those achieved for industrial brown juice. The findings from gel electrophoresis (SDS-PAGE), mass spectrometry, and reverse phase high-performance liquid chromatography (RP-HPLC) revealed that RuBisCO was obtained at a high purity of about 90 %. RuBisCO was hydrolyzed using pepsin and the resulting hydrolysate was assayed for antioxidative activity using various antioxidant assays, including the inhibition of linoleic acid oxidation, reduction of ferric ion, and stabilization of ABTS *? radicals. Overall, the results showed that RuBisCO could be considered as a promising alternative source for the production of novel industrial peptides for application in the pharmaceutical, nutraceutical and functional food industries.

Research paper thumbnail of Peptide from RuBisCO protein hydrolysate and its application in beef meat preservation

Pesquisa Agropecuaria Brasileira, Dec 31, 2022

Research paper thumbnail of Physicochemical and Biological Properties of Eel By-Products Protein Hydrolysates: Potential Application to Meat Product Preservation

Waste and Biomass Valorization, Aug 13, 2018

European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides wit... more European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides with antibacterial and antioxidant activity. Protein hydrolysates from Anguilla by-27 products obtained by treatment with Savinase® (PHAB-s) have high protein content than that 28 produced with Protamex ® (PHAB-p). Furthemore, both protein hydrolysates had a high 29 percentage of essential amino acids. In addition, results indicated that PHAB-s and PHAB-p 30 exhibited different degrees of antioxidant activities, evaluated by several antioxidant assays. 31 Thus, PHAB-s displayed high antioxidant activity, in terms of DPPH-radical scavenging effect 32 (IC50=5.2 mg/ml), β-carotene bleaching inhibitory effect (CI50=4.4 mg/ml) and metal chelating 33 activity. At 10 mg/ml, PHAB-p showed the highest reducing power. Antibacterial activities of 34 PHAB-s and PHAB-p were also evaluated. PHAB-s showed the most important inhibitory effects 35 against seven bacterial species. The application of PHAB-s and PHAB-p on minced meat as 36 preservative reduced the lipid oxidation and inhibited the microbial growth during chilled storage 37 for eleven days. 38 Statement of Novelty 39 Fish processing wastes are one of the important sources of natural bioactive compounds. 40 The recovery of these valuable components would not only improve the economy for fish 41 processors, but also would minimize the pollution potential of the fish processing waste. 42 Recently, our research group has been looking for new bioactive compounds from marine by-43 products. Further exploration of protein hydrolysates and peptides in other marine species can 44 provide a wider insight into studies on their biological and functional proprieties. In this context, 45 the objective of this investigation was to study bioactive potential of protein hydrolysates from 46 European eel (Anguilla anguilla) by-products.

Research paper thumbnail of Synthesis and antibacterial activity of new peptides from Alfalfa RuBisCO protein hydrolysates and mode of action via a membrane damage mechanism against Listeria innocua

Microbial pathogenesis, Jan 6, 2017

In this work we evaluated the mode of action of six new synthesized peptides (Met-Asp-Asn; Glu-le... more In this work we evaluated the mode of action of six new synthesized peptides (Met-Asp-Asn; Glu-leu-Ala-Ala-Ala-Cys; Leu-Arg-Asp-Asp-Phe; Gly-Asn-Ala-Pro-Gly-Ala-Val-Ala; Ala-Leu-Arg-Met-Ser-Gly and Arg-Asp-Arg-Phe-Leu), previously identified, from the most active peptide fractions of RuBisCO peptic hydrolysate against Listeria innocua via a membrane damage mechanism. Antibacterial effect and the minimum inhibitory concentrations (MIC) of these peptides were evaluated against six strains and their hemolytic activities towards bovine erythrocytes were determined. Prediction of the secondary structure of peptides indicated that these new antibacterial peptides are characterized by a short peptide chains (3-8 amino acid) and a random coli structure. Moreover, it was observed that one key characteristic of antibacterial peptides is the presence of specific amino acids such as cysteine, glycine, arginine and aspartic acid. In addition the determination of the extracellular potassium conce...

Research paper thumbnail of Identification and molecular docking of novel ACE inhibitory peptides from protein hydrolysates of shrimp waste

Engineering in Life Sciences, 2018

The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive... more The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive activity of shrimp waste proteins was evaluated. The physicochemical characterization, interfacial tension, and surface characteristics of shrimp waste protein hydrolysates (SWPH) using different enzyme/substrate (E/S) (SWPH 5 (SWPH using E/S = 5), SWPH 15 (SWPH using E/S = 15), and SWPH 40 (SWPH using E/S = 40)) were also studied. SWPH 5 , SWPH 15 , and SWPH 40 had an isoelectric pH around 2.07, 2.17, and 2.54 respectively. SWPH 5 exhibited the lowest interfacial tension (68.96 mN/m) followed by SWPH 15 (69.36 mN/m) and SWPH 40 (70.29 mN/m). The in vitro ACE inhibitory activity of shrimp waste protein hydrolysates showed that the most active hydrolysate was obtained using an enzyme/substrate of 15 U/mg (SWPH 15). SWPH 15 had a lower IC 50 value (2.17 mg/mL) than that of SWPH 5 and SWPH 40 (3.65 and 5.7 mg/mL, respectively). This hydrolysate was then purified and characterized. Fraction F1 separated by Sephadex G25 column which presents the best ACE inhibition activity was then separated by reversed-phase high performance liquid chromatography. Four ACE inhibitory peptides were identified and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were SSSKAKKMP, HGEGGRSTHE, WLGHGGRPDHE, and WRMDIDGDIMISE-QEAHQR. The structural modeling of anti-ACE peptides from shrimp waste through docking simulations results showed that these peptides bound to ACE with high affinity.

Research paper thumbnail of Physicochemical and Biological Properties of Eel By-Products Protein Hydrolysates: Potential Application to Meat Product Preservation

Waste and Biomass Valorization, 2018

European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides wit... more European eel by-products have been hydrolysed in order to produce novel bioactive 26 peptides with antibacterial and antioxidant activity. Protein hydrolysates from Anguilla by-27 products obtained by treatment with Savinase® (PHAB-s) have high protein content than that 28 produced with Protamex ® (PHAB-p). Furthemore, both protein hydrolysates had a high 29 percentage of essential amino acids. In addition, results indicated that PHAB-s and PHAB-p 30 exhibited different degrees of antioxidant activities, evaluated by several antioxidant assays. 31 Thus, PHAB-s displayed high antioxidant activity, in terms of DPPH-radical scavenging effect 32 (IC50=5.2 mg/ml), β-carotene bleaching inhibitory effect (CI50=4.4 mg/ml) and metal chelating 33 activity. At 10 mg/ml, PHAB-p showed the highest reducing power. Antibacterial activities of 34 PHAB-s and PHAB-p were also evaluated. PHAB-s showed the most important inhibitory effects 35 against seven bacterial species. The application of PHAB-s and PHAB-p on minced meat as 36 preservative reduced the lipid oxidation and inhibited the microbial growth during chilled storage 37 for eleven days. 38 Statement of Novelty 39 Fish processing wastes are one of the important sources of natural bioactive compounds. 40 The recovery of these valuable components would not only improve the economy for fish 41 processors, but also would minimize the pollution potential of the fish processing waste. 42 Recently, our research group has been looking for new bioactive compounds from marine by-43 products. Further exploration of protein hydrolysates and peptides in other marine species can 44 provide a wider insight into studies on their biological and functional proprieties. In this context, 45 the objective of this investigation was to study bioactive potential of protein hydrolysates from 46 European eel (Anguilla anguilla) by-products.

Research paper thumbnail of Antibacterial activity of novel peptides isolated from protein hydrolysates of RuBisCO purified from green juice alfalfa

Journal of Functional Foods, 2015

The present paper reports on the isolation and characterization of novel bioactive peptides by th... more The present paper reports on the isolation and characterization of novel bioactive peptides by the hydrolysis of RuBisCO proteins. Antibacterial activity was evaluated against Gramnegative (Escherichia coli and Salmonella enterica) and Gram-positive (Listeria innocua, Staphylococcus aureus, Enterococcus faecalis, Micrococcus luteus and Bacillus subtilis) bacteria. A simple method, consisting in the precipitation of the active peptides under ionic strength and pH conditions, was used to separate antimicrobial peptides from complex peptic hydrolysates of RuBisCO obtained by treatment with Pepsin ® (DH = 18.8%). The obtained peptide extract was fractionated using RP-HPLC, and the active fractions were analyzed by liquid chromatography, electrospray ionization, and tandem mass spectrometry (LC-ESI-MS). Among a total of twelve identified peptides, three new pure antibacterial peptides, namely (Met-Asp-Asn), (Glu-Leu-Ala-Ala-Ala-Cys) and (Leu-Arg-Asp-Asp-Phe), were obtained under hydrolytic conditions. The latter peptides were highly active against the tested strains. The three peptides did not show hemolytic activity towards bovine erythrocytes. Taken together, the results suggest that the new peptides isolated from RuBisCO could offer potential ingredients for the functional foods industry.

Research paper thumbnail of Purification de la RuBisCO à partir de la Luzerne, hydrolyse enzymatique, identification, structure-fonction des peptides bioactifs et leur valorisation dans des produits alimentaires

La luzerne est la plante la plus cultivee dans le monde et est une excellente source de proteines... more La luzerne est la plante la plus cultivee dans le monde et est une excellente source de proteines. Cependant, la RuBisCO a montre le plus d'interet. Cette proteine a ete etiquetee comme la proteine la plus abondante sur terre, elle constitue environ 65% (p/p) des proteines solubles de la luzerne. Dans ce travail une nouvelle methode a ete mise en place pour la purification de la RuBisCO a partir de la poudre de luzerne 10% (p/v) en utilisant deux solvants differents et l’effet du pH. Premierement, des methodes d’analyse qualitative et quantitative ont ete utilisees pour confirmer l’efficacite de la methode de purification qui pourrait remplacer certains procedes industriels classiques. Puis, une hydrolyse pepsique a ete realisee sur la RuBisCO purifiee qui aboutit a une forte population peptidique bioactive. Les peptides finaux de 24h d’hydrolyse ont montre une meilleure activite antibacterienne ou antioxydante par rapport aux autres hydrolysats. 9 nouveaux peptides antibacterie...

Research paper thumbnail of Purification and Recovery of RuBisCO Protein from Alfalfa Green Juice: Antioxidative Properties of Generated Protein Hydrolysate

Waste and Biomass Valorization, 2016

RuBisCO is the main soluble protein of alfalfa juice. Despite its attractive applications in a wi... more RuBisCO is the main soluble protein of alfalfa juice. Despite its attractive applications in a wide range of food products this protein remains largely underutilized. Accordingly, the present study was undertaken to investigate the feasibility and efficiency of using ammonium hydroxide (NH 4 OH) and precipitation at pH 3 for purification and recovery of RuBisCO from industrial Alfalfa green juice. The performance of the proposed RuBisCO recovery method was evaluated through qualitative and quantitative analysis. The results obtained by the proposed method were compared to those achieved for industrial brown juice. The findings from gel electrophoresis (SDS-PAGE), mass spectrometry, and reverse phase high-performance liquid chromatography (RP-HPLC) revealed that RuBisCO was obtained at a high purity of about 90 %. RuBisCO was hydrolyzed using pepsin and the resulting hydrolysate was assayed for antioxidative activity using various antioxidant assays, including the inhibition of linoleic acid oxidation, reduction of ferric ion, and stabilization of ABTS *? radicals. Overall, the results showed that RuBisCO could be considered as a promising alternative source for the production of novel industrial peptides for application in the pharmaceutical, nutraceutical and functional food industries.