Sadaf Asaei - Academia.edu (original) (raw)

Papers by Sadaf Asaei

Health Science Reports

2.2 | Study area and sample size Samples of these individuals were taken from six different healt... more 2.2 | Study area and sample size Samples of these individuals were taken from six different health centers in Fars province and then transferred to PACMRC. Based on the 11% of COVID-19 infection rate at the time of the study in PACMRC, a minimal sample size of 151 was estimated (95% confidence interval [CI], 5% margin of error). However, at least 1373 samples were required to achieve such a population, but 1895 samples were tested. Of the 1895 samples, 187 were positive for

Archives of Pediatric Infectious Diseases

Background: Given that immunocompromised patients are more at risk for the infection of SARS-CoV-... more Background: Given that immunocompromised patients are more at risk for the infection of SARS-CoV-2, epidemiological data are critical for assessing the corresponding prevalence among health care workers (HCWs) and patients at health centers. Objectives: This study aims to investigate the prevalence of SARS-CoV-2 infection among the staff of two hospitals that take care of immunocompromised patients, including pediatrics and adults with special medical conditions. Methods: This cross-sectional study includes all HCWs of the two hospitals; Abu Ali Sina Transplant Hospital (AASTH) and Amir al-Momenin Burn Injury Hospital (AABIH) in Shiraz, southern Iran, conducted from April 11, 2020, to June 16, 2021. The TaqMan real-time PCR assay was used to assess the SARS-CoV-2 infection rate in the suspected HCWs. Results: Out of 1232 sampled HCWs, 694 (56%) were female. Two hundred sixty-five samples (21.5%) and 967 samples (78.5%) were prepared from AABIH, and AASTH, respectively. The results s...

Parasites & Vectors

Background The magnitude of the health problems caused by leishmaniasis has been a major driving ... more Background The magnitude of the health problems caused by leishmaniasis has been a major driving factor behind the development and implementation of leishmaniasis control programs by the national authorities in Iran, with a priority for health and environmental management. Such programs are not achievable unless all of the factors leading to the infection, including the parasite’s life-cycle, vectors and reservoirs, are recognized. So far in Iran, humans and rodents have been considered the principal reservoirs of Leishmania tropica and Leishmania major, respectively, both associated with cutaneous leishmaniasis (CL), with domestic dogs considered to be the main reservoir for Leishmania infantum, associated with visceral leishmaniasis (VL). The role of other mammals in maintaining the Leishmania parasite has remained unclear. This study aimed to investigate Leishmania infection among livestock in endemic areas of VL and CL in Fars province, southern Iran, using serological and molec...

Objective: The purpose of this study was to investigate the prevalence of non-polio enteroviral i... more Objective: The purpose of this study was to investigate the prevalence of non-polio enteroviral infections by quantitative and qualitative PCR assays in neonates 3-30 days old with clinical diagnosis of sepsis. Methods: The patients included all the neonates aged 3-30 days admitted with diagnosis of neonatal sepsis. Blood specimens, throat swabs, and in 60 cases CSF specimens were tested for Enteroviruses (EVs), using a quantitative RT-PCR assay. Alternate RT-PCR assays separated polio and non-polio enteroviruses. Demographic data including age, sex, type of feeding and gestational age were obtained. Results: As PCR results revealed, of the 177 neonates with clinical diagnosis of sepsis, one patient (0.55%) was positive for non-polio EVs in all of throat, serum and CSF samples. In 3 patients (1.7%), the results were positive for non-polio EVs in both throat and serum, while in15 (8.45%) others only throat samples were positive. Sex, age and gestational age had no significant relatio...

Background & Objective: Herpes viruses can cause diseases in the clinical range. The virus can ca... more Background & Objective: Herpes viruses can cause diseases in the clinical range. The virus can cause infection in various body parts, especially eyes and nervous system. The aim of this study was at evaluating the Real-Time TaqMan probe PCR in diagnosing and monitoring of the patients with suspected HSV infections. Materials & Methods: More than a thousand patients with suspected HSV infections were collected. The samples were analyzed by Real-time PCR assays. DNA was extracted according to manufacturer's instruction (Invisorb Spin Virus DNA mini Kit, Germany(. The Real-Time PCR assay was performed with the primer Design TM genesis for herpes simplex virus (Primer Design, UK(.

Archives of Pediatric Infectious Diseases, 2022

Background: Childhood bacterial meningitis (BM) requires prompt and precise diagnosis to provide ... more Background: Childhood bacterial meningitis (BM) requires prompt and precise diagnosis to provide proper treatment and decline mortality and morbidity. Objectives: We aimed to evaluate the World Health Organization (WHO) criteria and polymerase chain reaction (PCR) for diagnosing BM in children admitted to a tertiary referral hospital in Shiraz, southern Iran. Materials: We included all 492 children aged one month to 17 years suspected of meningitis who had cerebrospinal fluid (CSF) leukocytosis admitted to Nemazi Hospital from August 2016 to September 2017. The CSF specimens were examined for routine analysis, Gram staining, and culture. A multiplex real-time PCR was used to identify Streptococcus pneumoniae, Haemophilus influenzae type b (Hib), and Neisseria meningitidis in the CSF samples. Seven viruses were also investigated using real-time PCR. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated using the WHO criteri...

Background & Objective: Host resistance towards Leishmania infection is mediated by cellular immu... more Background & Objective: Host resistance towards Leishmania infection is mediated by cellular immune responses leading to macrophage activation and parasite killing. According to the important role of IL-13 in the defense against visceral leishmaniasis)VL(and the known effect of the IL-13 gene polymorphisms on its production, the aim of this study was to investigate the probable relationship between IL-13 gene polymorphisms and susceptibility to VL. Materials & Methods: The patient group included 52 patients who had suffered from VL infection and the control group consisted of 104 non-relative healthy people from the same endemic areas the patients were from)southern part of Fars Province(. IL-13)position-1512 A/C(gene polymorphism was determined by polymerase chain reaction-restricted fragment length polymorphism)PCR-RFLP(. Results: There was no significant association between the frequencies of IL-13 (-1512) alleles and genotypes in the patients with VL compared to the thenormal population. Conclusion: This study indicated that the IL-13)position-1512 A/C(genotypes cannot be considered as a genetic susceptibility factor for leishmaniasis.

Archives of Clinical Infectious Diseases

Background: Interleukin-17A (IL-17A) gene can be a potential candidate gene implicated in viscera... more Background: Interleukin-17A (IL-17A) gene can be a potential candidate gene implicated in visceral leishmaniasis (VL), a disease caused by an infection with Leishmania parasite. Objectives: The aim of this study was to explore whether there is an association between IL-17A polymorphisms and VL in the Iranian population. Methods: A total of 202 participants (55 VL patients and 125 healthy controls) were investigated in the present case-control study. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of IL-17A rs3819024, rs3819025, and rs8193038 A alleles, and haplotype AGAG were significantly higher in the controls than patients (P = 0.0006, 0.017, 0.0003 and 0.001, respectively), while IL-17A rs3748067 A allele distribution was higher in patients than controls (P = 0.00004). Also, the frequencies of AA genotypes of rs3819024, rs3819025 and rs8193038 were higher in the controls (P = 0.0048, 0.014, and 0.018, respectively) while rs3748067 AA genotype was of greater distribution in the patients (P = 0.000048). Conclusions: The findings highlighted the role of IL-17A in the pathogenesis of the VL in humans.

On the subject of brucellosis, it seems that Th1/Th2 cytokines balance may be involved in the res... more On the subject of brucellosis, it seems that Th1/Th2 cytokines balance may be involved in the resistance or susceptibility to Brucella infection. In this respect, Th1 cytokines confer resistance, while Th2 cytokines predispose brucellosis. It is also clarified that IL-17 is required for the induction of IFN-c and IL-12 in macrophages and dendritic cells. Then, it seems that IL-17 can affect the induction of Th1 immunity which is necessary for controlling Brucella. In the present study, we tried to investigate probable relationship between IL-17A genetic variants and susceptibility to the human brucellosis. One hundred and seventy six patients with brucellosis and 84 healthy animal husbandmen, who consumed contaminated raw milk and dairy products from animals with brucellosis, were included in this study. All individuals were genotyped for 9 single nucleotide polymorphisms (SNPs) (rs4711998AG, rs8193036CT, rs3819024AG, rs2275913AG, rs3819025AG, rs8193038AG, rs3804513AT, rs1974226AG and rs3748067AG) being selected by using NCBI SNP database and literature using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The distribution of IL-17 rs4711998, rs8193038, rs3748067 AA genotypes and AAGAA haplotype were significantly more frequent in the patients than in the controls (P = 0.008, 0.0019, 0.003 and 0.002, respectively) while IL-17 genotypes rs3819024GG and rs3819025AA were more frequent in the controls than the patients (P = 0.001 and 0.0035, respectively). Based on the results, IL-17 rs4711998, rs8193038, rs3748067 AA genotypes and AAGAA haplotype could be considered as susceptibility factors for brucellosis while the inheritance of IL-17 rs3819024GG and rs3819025AA genotypes might be resistance factors against the disease.

Cytokine, 2013

On the subject of brucellosis, it seems that Th1/Th2 cytokines balance may be involved in the res... more On the subject of brucellosis, it seems that Th1/Th2 cytokines balance may be involved in the resistance or susceptibility to Brucella infection. In this respect, Th1 cytokines confer resistance, while Th2 cytokines predispose brucellosis. It is also clarified that IL-17 is required for the induction of IFN-γ and IL-12 in macrophages and dendritic cells. Then, it seems that IL-17 can affect the induction of Th1 immunity which is necessary for controlling Brucella. In the present study, we tried to investigate probable relationship between IL-17A genetic variants and susceptibility to the human brucellosis. One hundred and seventy six patients with brucellosis and 84 healthy animal husbandmen, who consumed contaminated raw milk and dairy products from animals with brucellosis, were included in this study. All individuals were genotyped for 9 single nucleotide polymorphisms (SNPs) (rs4711998AG, rs8193036CT, rs3819024AG, rs2275913AG, rs3819025AG, rs8193038AG, rs3804513AT, rs1974226AG and rs3748067AG) being selected by using NCBI SNP database and literature using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The distribution of IL-17 rs4711998, rs8193038, rs3748067 AA genotypes and AAGAA haplotype were significantly more frequent in the patients than in the controls (P=0.008, 0.0019, 0.003 and 0.002, respectively) while IL-17 genotypes rs3819024GG and rs3819025AA were more frequent in the controls than the patients (P=0.001 and 0.0035, respectively). Based on the results, IL-17 rs4711998, rs8193038, rs3748067 AA genotypes and AAGAA haplotype could be considered as susceptibility factors for brucellosis while the inheritance of IL-17 rs3819024GG and rs3819025AA genotypes might be resistance factors against the disease.

Molecular Biology Reports, 2012

Lymphotoxin-a (LT-a) and interleukin-1beta (IL-1b) are proinflammatory cytokines playing importan... more Lymphotoxin-a (LT-a) and interleukin-1beta (IL-1b) are proinflammatory cytokines playing important roles in immunity against Leishmania infection and the outcome of the disease. As cytokine productions are under the genetic control, this study tried to find any probable relationship between these cytokine gene polymorphisms and the susceptibility to visceral leishmaniasis in Iranian pediatric patients. Ninety-five pediatric patients involved with visceral leishmaniasis and 128 non-relative healthy people, from the same area as the patients, were genotyped for LT-a (?252A/G) and IL-1b (?3953T/C and-511T/C) gene polymorphisms using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). There was not found any significant differences in allele and genotype frequencies of LT-a (?252A/G) and IL-1b (?3953) among the study groups. However, the frequency of IL-1b-511TT genotype was higher in the controls (P = 0.0004) while the frequency of IL-1b-511CC genotype and C allele were higher in the patients (P = 0.008 and P = 0.00006, respectively). Furthermore, IL-1b CC (-511/?3953) haplotype was more frequent in VL patients compared with the controls (P = 0.0002) and the distribution of TT haplotype was higher in the controls compared with the patients (P = 0.003). In conclusion, based on the results, IL-1b-511C allele, CC genotype and CC (-511/?3953) haplotype could be considered as the susceptibility factors for visceral leishmaniasis while IL-1b-511TT genotype, T allele and TT haplotype (-511/ ?3953) might be counted as the influential factors for resistance to the disease.

Journal of Fasa University of Medical Sciences, Sep 15, 2013

Host resistance towards Leishmania infection is mediated by cellular immune responses leading to ... more Host resistance towards Leishmania infection is mediated by cellular immune responses leading to macrophage activation and parasite killing. According to the important role of IL-13 in the defense against visceral leishmaniasis )VL( and the known effect of the IL-13 gene polymorphisms on its production, the aim of this study was to investigate the probable relationship between IL-13 gene polymorphisms and susceptibility to VL.

Iranian journal of immunology: IJI

Background: Increased levels of interleukin-8 (IL-8) and interleukin-6 (IL-6) in acute human bruc... more Background: Increased levels of interleukin-8 (IL-8) and interleukin-6 (IL-6) in acute human brucellosis have been reported. Previous studies have shown that the production and level of IL-6 and IL-8 cytokines are associated with the polymorphism of the encoding genes. Objective: To investigate the probable association between IL-6 (-174 C/G) and IL-8 (-251 A/T) gene polymorphisms and susceptibility/resistance to brucellosis. Methods: The patient group included 196 patients suffering from Brucella infection and the control group consisted of 82 healthy animal husbandmen from the same geographical area. IL-8 (-251 A/C) and IL-6 (-174 C/G) gene polymorphisms were analyzed by PCR-RFLP and Allele Specific PCR (AS-PCR) respectively. Results: The frequency of -251 IL-8 AA genotype was significantly lower in the controls compared with that of the patients (p=0.0051), while the frequencies of other genotypes (AT and TT) and alleles (A and T) were not significantly different among the partic...

Molecular Biology Reports, 2012

Lymphotoxin-a (LT-a) and interleukin-1beta (IL-1b) are proinflammatory cytokines playing importan... more Lymphotoxin-a (LT-a) and interleukin-1beta (IL-1b) are proinflammatory cytokines playing important roles in immunity against Leishmania infection and the outcome of the disease. As cytokine productions are under the genetic control, this study tried to find any probable relationship between these cytokine gene polymorphisms and the susceptibility to visceral leishmaniasis in Iranian pediatric patients. Ninety-five pediatric patients involved with visceral leishmaniasis and 128 non-relative healthy people, from the same area as the patients, were genotyped for LT-a (?252A/G) and IL-1b (?3953T/C and -511T/C) gene polymorphisms using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). There was not found any significant differences in allele and genotype frequencies of LT-a (?252A/G) and IL-1b (?3953) among the study groups. However, the frequency of IL-1b -511TT genotype was higher in the controls (P = 0.0004) while the frequency of IL-1b -511CC genotype and C allele were higher in the patients (P = 0.008 and P = 0.00006, respectively). Furthermore, IL-1b CC (-511/?3953) haplotype was more frequent in VL patients compared with the controls (P = 0.0002) and the distribution of TT haplotype was higher in the controls compared with the patients (P = 0.003). In conclusion, based on the results, IL-1b -511C allele, CC genotype and CC (-511/?3953) haplotype could be considered as the susceptibility factors for visceral leishmaniasis while IL-1b -511TT genotype, T allele and TT haplotype (-511/ ?3953) might be counted as the influential factors for resistance to the disease.

Molecular Biology Reports, 2013

Host resistance to Leishmania infection is mediated by cellular immune responses leading to macro... more Host resistance to Leishmania infection is mediated by cellular immune responses leading to macrophage activation and parasite killing. Interleukin-18 (IL-18) known as interferon-c (IFN-c) inducing factor, stimulates IFN-c production by T cells. Taking into account the important role of IL-18 in the defense against visceral leishmaniasis (VL) and the known effect of IL-18 gene polymorphisms on its production, the aim of this study was to investigate the probable relationship between IL-18 gene polymorphisms and the susceptibility to VL. The study groups included 118 pediatric patients who suffered from VL and 156 non-relative healthy people as the controls from the same endemic area. IL-18 gene polymorphisms at the positions -656 G/T, -137 G/C and ?105A/C (codon 35/3) were analyzed by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP). The results showed that the frequency of T allele at the position -656 was significantly higher in the controls, compared with that in the patients (P = 0.047), but it couldn't tolerate Bonferroni correction. Regarding the IL-18 genotypes, there was no significant difference between the patients and controls. Although the frequencies of ATG single haplotype and AGG/ATG double haplotype were significantly higher in the controls (P = 0.043) and the patients (P = 0.044), respectively, the two P values couldn't tolerate Bonferroni correction. Furthermore, a strong linkage disequilibrium was observed among the -656, -137 and ?105 single nucleotide polymorphisms of IL-18 gene (all Ps \ 0.001). In conclusion, this study suggests that the inheritance of T allele at the position -656 may be considered as a genetic factor for resistance to VL.

International Journal of Infectious Diseases, 2012

Background: Lymphotoxin-␣ (LT-␣), interleukin-6 (IL-6) and interleukin-1beta (IL-1␤) are proinfla... more Background: Lymphotoxin-␣ (LT-␣), interleukin-6 (IL-6) and interleukin-1beta (IL-1␤) are proinflammatory cytokines playing important roles in immunity against Leishmania infection and the outcome of the disease. As cytokine productions are under the genetic control, this study tried to find any probable relationship between these cytokine gene polymorphisms and the susceptibility to visceral leishmaniasis (VL) in Iranian pediatric patients. Methods: 95 pediatric patients involved with visceral leishmaniasis and 128 non-relative healthy people, from the same area as the patients, were genotyped for LT-␣ (+252 A/G), IL-6 (-174 C/G) and IL-1␤ (+3953 T/C and-511T/C) gene polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: There was not found any significant differences in allele and genotype frequencies of LT-␣ (+252 A/G), IL-6 (-174 C/G) and IL-1␤ (+3953) among the study groups. However, the frequency of IL-1␤-511TT genotype was higher in the controls (P = 0.0004) while the frequency of IL-1␤-511CC genotype and C allele were higher in the patients (P = 0.008 and P = 0.00006, respectively). Furthermore, IL-1␤ CC (-511/ + 3953) haplotype was more frequent in VL patients compared with the controls (P = 0.0002) and the distribution of TT haplotype was higher in controls compared with the patients (P = 0.003). Conclusion: Based on the results IL-1␤-511C allele, CC genotype and CC (-511/ + 3953) haplotype could be considered as the susceptibility factors for visceral leishmaniasis while IL-1␤-511TT genotype, T allele and TT haplotype (-511/ + 3953) might be counted as the influential factors for resistance to the disease.

Cytokine, 2013

Health Science Reports

Archives of Pediatric Infectious Diseases

Parasites & Vectors

Archives of Pediatric Infectious Diseases, 2022

Archives of Clinical Infectious Diseases

Cytokine, 2013

On the subject of brucellosis, it seems that Th1/Th2 cytokines balance may be involved in the res... more On the subject of brucellosis, it seems that Th1/Th2 cytokines balance may be involved in the resistance or susceptibility to Brucella infection. In this respect, Th1 cytokines confer resistance, while Th2 cytokines predispose brucellosis. It is also clarified that IL-17 is required for the induction of IFN-γ and IL-12 in macrophages and dendritic cells. Then, it seems that IL-17 can affect the induction of Th1 immunity which is necessary for controlling Brucella. In the present study, we tried to investigate probable relationship between IL-17A genetic variants and susceptibility to the human brucellosis. One hundred and seventy six patients with brucellosis and 84 healthy animal husbandmen, who consumed contaminated raw milk and dairy products from animals with brucellosis, were included in this study. All individuals were genotyped for 9 single nucleotide polymorphisms (SNPs) (rs4711998AG, rs8193036CT, rs3819024AG, rs2275913AG, rs3819025AG, rs8193038AG, rs3804513AT, rs1974226AG and rs3748067AG) being selected by using NCBI SNP database and literature using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The distribution of IL-17 rs4711998, rs8193038, rs3748067 AA genotypes and AAGAA haplotype were significantly more frequent in the patients than in the controls (P=0.008, 0.0019, 0.003 and 0.002, respectively) while IL-17 genotypes rs3819024GG and rs3819025AA were more frequent in the controls than the patients (P=0.001 and 0.0035, respectively). Based on the results, IL-17 rs4711998, rs8193038, rs3748067 AA genotypes and AAGAA haplotype could be considered as susceptibility factors for brucellosis while the inheritance of IL-17 rs3819024GG and rs3819025AA genotypes might be resistance factors against the disease.

Molecular Biology Reports, 2012

Journal of Fasa University of Medical Sciences, Sep 15, 2013

Iranian journal of immunology: IJI

Molecular Biology Reports, 2012

Lymphotoxin-a (LT-a) and interleukin-1beta (IL-1b) are proinflammatory cytokines playing importan... more Lymphotoxin-a (LT-a) and interleukin-1beta (IL-1b) are proinflammatory cytokines playing important roles in immunity against Leishmania infection and the outcome of the disease. As cytokine productions are under the genetic control, this study tried to find any probable relationship between these cytokine gene polymorphisms and the susceptibility to visceral leishmaniasis in Iranian pediatric patients. Ninety-five pediatric patients involved with visceral leishmaniasis and 128 non-relative healthy people, from the same area as the patients, were genotyped for LT-a (?252A/G) and IL-1b (?3953T/C and -511T/C) gene polymorphisms using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). There was not found any significant differences in allele and genotype frequencies of LT-a (?252A/G) and IL-1b (?3953) among the study groups. However, the frequency of IL-1b -511TT genotype was higher in the controls (P = 0.0004) while the frequency of IL-1b -511CC genotype and C allele were higher in the patients (P = 0.008 and P = 0.00006, respectively). Furthermore, IL-1b CC (-511/?3953) haplotype was more frequent in VL patients compared with the controls (P = 0.0002) and the distribution of TT haplotype was higher in the controls compared with the patients (P = 0.003). In conclusion, based on the results, IL-1b -511C allele, CC genotype and CC (-511/?3953) haplotype could be considered as the susceptibility factors for visceral leishmaniasis while IL-1b -511TT genotype, T allele and TT haplotype (-511/ ?3953) might be counted as the influential factors for resistance to the disease.

Molecular Biology Reports, 2013

International Journal of Infectious Diseases, 2012

Cytokine, 2013