Sajeeb Khan - Academia.edu (original) (raw)
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Papers by Sajeeb Khan
Environmental and Experimental Biology
Pseudomonas aeruginosa is a gram-negative, motile and clinically relevant bacterium. It is a sign... more Pseudomonas aeruginosa is a gram-negative, motile and clinically relevant bacterium. It is a significant pathogen associated with immunosuppression, cystic fibrosis and malignancy. Infections due to multidrug-resistant P. aeruginosa cause greater morbidity, death rate, and increased healthcare expenses. Drug efflux is a prevalent mechanism of antibiotic resistance in P. aeruginosa. The objective of the study was to analyze the antibiotic susceptibility profile of a clinical strain of P. aeruginosa (GC14) and to identify the mechanism of antibiotic resistance. The antibiotic resistance of GC14 was tested against 19 antibiotics using an antibiotic susceptibility test. A polymerase chain reaction-based strategy was employed to detect the presence of mexB, mexD and mexY efflux pump genes. The efflux pump inhibition assay was conducted to analyze the effect of efflux pumps on antibiotic resistance in GC14, using carbonyl cyanide m‑chlorophenyl hydrazone (CCCP) as an efflux pump inhibitor...
Environmental and Experimental Biology
Pseudomonas aeruginosa is a gram-negative, motile and clinically relevant bacterium. It is a sign... more Pseudomonas aeruginosa is a gram-negative, motile and clinically relevant bacterium. It is a significant pathogen associated with immunosuppression, cystic fibrosis and malignancy. Infections due to multidrug-resistant P. aeruginosa cause greater morbidity, death rate, and increased healthcare expenses. Drug efflux is a prevalent mechanism of antibiotic resistance in P. aeruginosa. The objective of the study was to analyze the antibiotic susceptibility profile of a clinical strain of P. aeruginosa (GC14) and to identify the mechanism of antibiotic resistance. The antibiotic resistance of GC14 was tested against 19 antibiotics using an antibiotic susceptibility test. A polymerase chain reaction-based strategy was employed to detect the presence of mexB, mexD and mexY efflux pump genes. The efflux pump inhibition assay was conducted to analyze the effect of efflux pumps on antibiotic resistance in GC14, using carbonyl cyanide m‑chlorophenyl hydrazone (CCCP) as an efflux pump inhibitor...