Samara Eberlin - Academia.edu (original) (raw)

Papers by Samara Eberlin

Surgical and Cosmetic Dermatology, 2022

Photodermatology, Photoimmunology and Photomedicine, Dec 1, 2008

Exposure to ultraviolet (UV) radiation induces generation of reactive oxygen species, production ... more Exposure to ultraviolet (UV) radiation induces generation of reactive oxygen species, production of proinflammatory cytokines and melanocyte-stimulating hormone (MSH) as well as increase in tyrosinase activity. The potential photoprotective effects of Coccoloba uvifera extract (CUE) were evaluated in UV-stimulated melanocytes. Human epidermal melanocytes were used as an in vitro model to evaluate the effects of CUE on the production interleukin-1alpha (IL-1alpha), tumor necrosis factor alpha (TNF-alpha), and alpha-MSH under basal and UV-stimulated conditions. Antioxidant and anti-tyrosinase activities were also evaluated in membrane lipid peroxidation and mushroom tyrosinase assay, respectively. Coccoloba uvifera L. showed antioxidant and anti-tyrosinase activities and also inhibited the production of IL-1alpha, TNF-alpha and alpha-MSH in melanocytes subjected to UV radiation (P<0.01). Moreover, CUE inhibited the activity of tyrosine kinase in cell culture under basal and UV radiation conditions (P<0.001), corroborating the findings of the mushroom tyrosinase assay. This study supports the photoprotective potential of CUE.

Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality... more Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality. Objective: To evaluate the reduction of abdominal fat in volunteers who underwent low-frequency diode laser therapy. Methods: A total of 60 volunteers (18 to 50 years of age, with a BMI of between 18.5 kg/m2 and 30 kg/m2, and who had abdominal fat), underwent a treatment performed twice a week for 31 days and were then evaluated. The abdominal circumference was evaluated in three locations, with photographic and ultrasound images taken of the studied area. Results: There was a reduction in the abdominal circumference measurements in the evaluated sites, with dermal compression and a significant reduction of hypodermis verified through ultrasound. Conclusion: The low-frequency diode laser therapy showed significant results in the

International journal of cosmetic science, Jan 20, 2015

Most of the cosmetic compounds with preservative properties available in the market pose some ris... more Most of the cosmetic compounds with preservative properties available in the market pose some risks concerning safety, such as the possibility of causing sensitization. Due to the fact that there are few options, the proper development of new molecules with this purpose is needed. Xylitol is a natural sugar, and the antimicrobial properties of xylitol-derived compounds have already been described in the literature. C-8 xylitol monoester and xylitol phosphate esters may be useful for the development of skincare products. As an initial screen for safety of chemicals, the combination of in silico methods and in vitro testing can aid in prioritizing resources in toxicological investigations while reducing the ethical and monetary costs that are related to animal and human testing. This study was designed to evaluate the safety of C-8 xylitol monoester and xylitol phosphate esters regarding carcinogenicity, mutagenicity, skin and eye irritation/corrosion and sensitization through alterna...

Surgical and Cosmetic Dermatology, 2015

Journal of the American Academy of Dermatology, 2020

Native human skin has been reported in the literature as being an important experimental model fo... more Native human skin has been reported in the literature as being an important experimental model for studying skin biology. Studies performed by our group have shown that <i>ex vivo</i> skin, from elective plastic surgery, maintains the biological characteristics of native skin under specific culture conditions. As such, it might be a feasible model for the safety and efficacy testing of topical substances. While Brazil is at the forefront of global regulation implementation, Brazilian researchers are not always able to transfer certain widely used protocols to their laboratories, particularly protocols that involve the use of reconstructed tissues with limited viability, such as those for skin corrosion (OECD TG 431) and irritation testing (OECD TG 439). In this study, we investigated the applicability of the <i>ex vivo</i> skin model to the evaluation of irritation and corrosion potential of a number of proficiency substances described in TG 431 and TG 439. T...

O modelo experimental de infeccao por Listeria monocytogenes tem provado ser util na investigacao... more O modelo experimental de infeccao por Listeria monocytogenes tem provado ser util na investigacao dos efeitos de novos compostos na resposta imunologica primaria contra bacterias intracelulares. Em particular, o numero de celulas hematopoeticas na medula ossea e baco e de fundamental importância na resistencia a esta infeccao. Neste trabalho, investigamos o efeito do extrato de Uncaria tomentosa (EUT) sobre o crescimento e diferenciacao de precursores hematopoeticos para granulocitos e macrofagos (CFU-GM) da medula ossea e do baco e a atividade estimuladora de colonias (CSA) do soro de animais normais e infectados com Listeria monocytogenes. A eficacia de EUT frente a uma dose letal da bacteria tambem foi avaliada. Demonstramos que o EUT protegeu os animais contra uma dose letal de L. monocytogenes, quando administrado profilaticamente nas doses de 50 e 100 mg/kg durante 7 dias, com taxas de sobrevida de 15 e 35%, respectivamente. Este tratamento tambem preveniu a mielossupressao e hematopoese extramedular causadas por uma dose sub-letal de L. monocytogenes, devido a um aumento no numero de CFU-GM na medula ossea. Alem disso, observamos uma resposta dose-dependente na estimulacao da mielopoese. Animais somente tratados (nao infectados) com uma a dose de 100mg/Kg tambem apresentaram numeros elevados de CFU-GM na medula ossea em relacao ao grupo controle. Adicionalmente, a investigacao da producao de fatores estimuladores de colonias no soro dos animais revelou um aumento da CSA nos grupos normais e infectados quando estes foram pre-tratados com EUT, sugerindo que a estimulacao da mielopoese pelo extrato seja mediada pela producao de fatores de crescimento. Estes resultados favorecem a nossa hipotese de que a habilidade da Uncaria tomentosa em induzir uma alta reserva de progenitores na medula ossea seja o maior determinante da resistencia a infeccao Abstract

Antioxidants, 2019

The aim of this study was to develop a phytocosmetic sunscreen emulsion with antioxidant effect, ... more The aim of this study was to develop a phytocosmetic sunscreen emulsion with antioxidant effect, containing a blend of flavonoid-enriched plant extracts. In vitro sun protection factor, antioxidant activity, skin irritation, photostability, cutaneous permeation, and retention of flavonoids were evaluated. Thermodynamically stable emulsions were obtained and tested for sensorial analysis after loading the blend of extracts. The selected emulsion was stable when stored at low temperatures (5 C), for which after 120 days the concentration of quercetin and rutin were above their limit of quantification, i.e., 2.8 ± 0.39 µg/mL and 30.39 ± 0.39 µg/mL, respectively. Spreadability, low rupture strength and adhesiveness were shown to be similar to a conventional topical product. Higher brittleness, pseudo-plastic, and viscoelastic behaviors were also recorded for the developed phytocosmetic sunscreen. The product presented a critical wavelength of 387.0 nm and ultraviolet rays A and B (UVA/U...

Supplemental Material, sj-docx-1-atl-10.1177_02611929211038652 for <i>Ex Vivo</i> Hum... more Supplemental Material, sj-docx-1-atl-10.1177_02611929211038652 for <i>Ex Vivo</i> Human Skin: An Alternative Test System for Skin Irritation and Corrosion Assays by Samara Eberlin, Gustavo Facchini, Gustavo Henrique da Silva, Samir Eberlin, Aline Rodrigues Bragatto, Ana Lúcia Tabarini Alves Pinheiro and Adriano da Silva Pinheiro in Alternatives to Laboratory Animals

Journal of The American Academy of Dermatology, 2020

Periorbital disobers (POD), cosmetic concern for large number of individuals, are characterized b... more Periorbital disobers (POD), cosmetic concern for large number of individuals, are characterized by an alteration in the color and shape of eyelids, manifested by hyperpigmentation (dark circles) and a safe and effective dermocosmetic product composed by concentration saponins and flavonoids, which corrects the multifonctional dissarrangement observed in POD physiopathology. Our results demonstrated that CEPPI reducted the levels of the main mediators of the inflammatory response, presented considerable lipolytic action, and promoted a stimuliting action on extracellular matrix protein synthesis. Clinical trials were also performed and demonstrated an improvement on dark circle tone, as measured by using a colorimetry assay, taken together, the results led us to conclude that CEPPL is an innovative and complete eyecare cosmetic indicated for dark circles and puffiness improvement.

Introducao: Introduction: Skin aging is a challenge to treat. Objectives: To evaluate the in vitr... more Introducao: Introduction: Skin aging is a challenge to treat. Objectives: To evaluate the in vitro and in vivo efficacy and clinical safety of a phytoestrogens-based cosmetic in the management of photoaging. Methods: The in vitro investigation was performed using the analysis of the genic expression of fibronectin and procollagen, evaluation of the immunomodulatory activity (proinflammatory and anti-inflammatory cytokines synthesis) and histochemistry and immunofluorescence analyses of the skin and the dermoepidermal junction. The in vivo investigation – performed in 76 women randomized into Group A (phytoestrogens complex cream and SPF 20 twice daily) or Group B (the same product plus a commercially available anti-aging cream applied overnight). The study lasted for 120 days, with physician- and patient-led evaluations, in addition to monthly ultrasound (20 MHz) and photographic analysis. Skin biopsies of the face were performed before and after treatment. Results: The study showed...

Desordens periorbitais (DPO) e um nome atribuido a um conjunto de disfuncoes esteticas caracteriz... more Desordens periorbitais (DPO) e um nome atribuido a um conjunto de disfuncoes esteticas caracterizadas por alteracoes na coloracao e na forma das palpebras. As principais manifestacoes observadas sao, hipercromia da regiao infraorbital, formacao de bolsas de gordura, de edema e de rugas finas. A hipercromia ocorre em funcao de um disturbio microvascular que pode gerar a formacao de edema e alteracao da tonalidade da pele. A formacao de bolsas de gordura e caracterizada por uma hipertrofia nos adipocitos devido ao acumulo de triacilglicerol intracelular. Pela acao deleteria da radiacao UV observa-se a degradacao do tecido conectivo, o que e refletido atraves do aparecimento de rugas ao redor dos olhos. Neste trabalho, avaliamos a possivel aplicacao da associacao dos extratos hidroglicolicos de Pfaffia paniculata e Ptychopetalum olacoides (AEHPPPO) na prevencao e tratamento da DPO. Os resultados clinicos obtidos demonstraram que AEHPPPO promoveu uma reducao da hipercromia, do edema e d...

People suffering from an ordinary acute cold consume so many handkerchiefs that the wiping action... more People suffering from an ordinary acute cold consume so many handkerchiefs that the wiping actions on their own increase the abrasive damage of the nasolabial zone, finally leading to a disturbed barrier function and inflamation. It seems that the quality of the material used for nose cleansing could play an important role and that innovative handkerchiefs would fulfil a preventive role in minimizing the damaging effect of the skin barrier function of the nasolabial zone during this conditions. The objective of this work was to evaluate the effects of a wet handkerchief (SKNW) on the skin barrier balance by measuring filaggrin and histamine using an experimental model of ex vivo native human skin model and the interference of this handkerchief in the skin microbiota through in vitro screening. SKNW showed an increase in the production of filaggrin and a reduction of histamine synthesis in human explants subjected to barrier disruption with 5% Sodium Lauryl Sulfate. Additionaly, SKNW...

Alternatives to Laboratory Animals

Native human skin has been reported in the literature as being an important experimental model fo... more Native human skin has been reported in the literature as being an important experimental model for studying skin biology. Studies performed by our group have shown that ex vivo skin, from elective plastic surgery, maintains the biological characteristics of native skin under specific culture conditions. As such, it might be a feasible model for the safety and efficacy testing of topical substances. While Brazil is at the forefront of global regulation implementation, Brazilian researchers are not always able to transfer certain widely used protocols to their laboratories, particularly protocols that involve the use of reconstructed tissues with limited viability, such as those for skin corrosion (OECD TG 431) and irritation testing (OECD TG 439). In this study, we investigated the applicability of the ex vivo skin model to the evaluation of irritation and corrosion potential of a number of proficiency substances described in TG 431 and TG 439. The skin fragments were standardised in...

Introducao: Hyperpigmentation is a common dermatological condition, usually associated with expos... more Introducao: Hyperpigmentation is a common dermatological condition, usually associated with exposure to the sun and relative difficulty of treatment. Pycnogenol® is an extract obtained from the French pine tree Pinus pinaster, with known antioxidant and anti-inflammatory activity, having been proven to inhibit melanin synthesis in previous studies. Objective: To evaluate the whitening activity of Pycnogenol® in an ex vivo experimental model after exposure to ultraviolet A and B, infrared-A radiations and visible light. Method: Human skin fragments obtained from elective plastic surgery were treated with Pycnogenol ® and then irradiated with ultraviolet A and B, and infrared-A radiations, and visible light, having been sent for histological evaluation of melanin pigmentation by the Fontana-Masson staining technique. Results: The histological evaluation demonstrated an increase in melanin deposition in all irradiated groups. However, fragments pre-incubated with Pycnogenol® showed a r...

Surgical and Cosmetic Dermatology, 2014

Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality... more Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality. Objective: To evaluate the reduction of abdominal fat in volunteers who underwent low-frequency diode laser therapy. Methods: A total of 60 volunteers (18 to 50 years of age, with a BMI of between 18.5 kg/m2 and 30 kg/m2, and who had abdominal fat), underwent a treatment performed twice a week for 31 days and were then evaluated. The abdominal circumference was evaluated in three locations, with photographic and ultrasound images taken of the studied area. Results: There was a reduction in the abdominal circumference measurements in the evaluated sites, with dermal compression and a significant reduction of hypodermis verified through ultrasound. Conclusion: The low-frequency diode laser therapy showed significant results in the reduction of localized fat and abdominal circumference measurements.

[

adjuvant to be used in dermocosmetic formulations. Clinical studies are in progress aiming to fur... more adjuvant to be used in dermocosmetic formulations. Clinical studies are in progress aiming to further investigate the protective effects of CGO in the skin [Maria Del Carmen Velazquez Pereda, Gustavo de Campos Dieamant, Samara Eberlin, Cecília Nogueira, Débora Colombi, Luiz Claudio Di Stasi and Mary Luci de Souza Queiroz (Department of Pharmacology/ Hemocenter, University of Campinas, Campinas, Brazil), J Cosmetic Dermatol, 2009, 8(1), 52-68].

Surgical and Cosmetic Dermatology, 2022

Photodermatology, Photoimmunology and Photomedicine, Dec 1, 2008

Exposure to ultraviolet (UV) radiation induces generation of reactive oxygen species, production ... more Exposure to ultraviolet (UV) radiation induces generation of reactive oxygen species, production of proinflammatory cytokines and melanocyte-stimulating hormone (MSH) as well as increase in tyrosinase activity. The potential photoprotective effects of Coccoloba uvifera extract (CUE) were evaluated in UV-stimulated melanocytes. Human epidermal melanocytes were used as an in vitro model to evaluate the effects of CUE on the production interleukin-1alpha (IL-1alpha), tumor necrosis factor alpha (TNF-alpha), and alpha-MSH under basal and UV-stimulated conditions. Antioxidant and anti-tyrosinase activities were also evaluated in membrane lipid peroxidation and mushroom tyrosinase assay, respectively. Coccoloba uvifera L. showed antioxidant and anti-tyrosinase activities and also inhibited the production of IL-1alpha, TNF-alpha and alpha-MSH in melanocytes subjected to UV radiation (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.01). Moreover, CUE inhibited the activity of tyrosine kinase in cell culture under basal and UV radiation conditions (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001), corroborating the findings of the mushroom tyrosinase assay. This study supports the photoprotective potential of CUE.

Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality... more Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality. Objective: To evaluate the reduction of abdominal fat in volunteers who underwent low-frequency diode laser therapy. Methods: A total of 60 volunteers (18 to 50 years of age, with a BMI of between 18.5 kg/m2 and 30 kg/m2, and who had abdominal fat), underwent a treatment performed twice a week for 31 days and were then evaluated. The abdominal circumference was evaluated in three locations, with photographic and ultrasound images taken of the studied area. Results: There was a reduction in the abdominal circumference measurements in the evaluated sites, with dermal compression and a significant reduction of hypodermis verified through ultrasound. Conclusion: The low-frequency diode laser therapy showed significant results in the

International journal of cosmetic science, Jan 20, 2015

Most of the cosmetic compounds with preservative properties available in the market pose some ris... more Most of the cosmetic compounds with preservative properties available in the market pose some risks concerning safety, such as the possibility of causing sensitization. Due to the fact that there are few options, the proper development of new molecules with this purpose is needed. Xylitol is a natural sugar, and the antimicrobial properties of xylitol-derived compounds have already been described in the literature. C-8 xylitol monoester and xylitol phosphate esters may be useful for the development of skincare products. As an initial screen for safety of chemicals, the combination of in silico methods and in vitro testing can aid in prioritizing resources in toxicological investigations while reducing the ethical and monetary costs that are related to animal and human testing. This study was designed to evaluate the safety of C-8 xylitol monoester and xylitol phosphate esters regarding carcinogenicity, mutagenicity, skin and eye irritation/corrosion and sensitization through alterna...

Surgical and Cosmetic Dermatology, 2015

Journal of the American Academy of Dermatology, 2020

Native human skin has been reported in the literature as being an important experimental model fo... more Native human skin has been reported in the literature as being an important experimental model for studying skin biology. Studies performed by our group have shown that <i>ex vivo</i> skin, from elective plastic surgery, maintains the biological characteristics of native skin under specific culture conditions. As such, it might be a feasible model for the safety and efficacy testing of topical substances. While Brazil is at the forefront of global regulation implementation, Brazilian researchers are not always able to transfer certain widely used protocols to their laboratories, particularly protocols that involve the use of reconstructed tissues with limited viability, such as those for skin corrosion (OECD TG 431) and irritation testing (OECD TG 439). In this study, we investigated the applicability of the <i>ex vivo</i> skin model to the evaluation of irritation and corrosion potential of a number of proficiency substances described in TG 431 and TG 439. T...

O modelo experimental de infeccao por Listeria monocytogenes tem provado ser util na investigacao... more O modelo experimental de infeccao por Listeria monocytogenes tem provado ser util na investigacao dos efeitos de novos compostos na resposta imunologica primaria contra bacterias intracelulares. Em particular, o numero de celulas hematopoeticas na medula ossea e baco e de fundamental importância na resistencia a esta infeccao. Neste trabalho, investigamos o efeito do extrato de Uncaria tomentosa (EUT) sobre o crescimento e diferenciacao de precursores hematopoeticos para granulocitos e macrofagos (CFU-GM) da medula ossea e do baco e a atividade estimuladora de colonias (CSA) do soro de animais normais e infectados com Listeria monocytogenes. A eficacia de EUT frente a uma dose letal da bacteria tambem foi avaliada. Demonstramos que o EUT protegeu os animais contra uma dose letal de L. monocytogenes, quando administrado profilaticamente nas doses de 50 e 100 mg/kg durante 7 dias, com taxas de sobrevida de 15 e 35%, respectivamente. Este tratamento tambem preveniu a mielossupressao e hematopoese extramedular causadas por uma dose sub-letal de L. monocytogenes, devido a um aumento no numero de CFU-GM na medula ossea. Alem disso, observamos uma resposta dose-dependente na estimulacao da mielopoese. Animais somente tratados (nao infectados) com uma a dose de 100mg/Kg tambem apresentaram numeros elevados de CFU-GM na medula ossea em relacao ao grupo controle. Adicionalmente, a investigacao da producao de fatores estimuladores de colonias no soro dos animais revelou um aumento da CSA nos grupos normais e infectados quando estes foram pre-tratados com EUT, sugerindo que a estimulacao da mielopoese pelo extrato seja mediada pela producao de fatores de crescimento. Estes resultados favorecem a nossa hipotese de que a habilidade da Uncaria tomentosa em induzir uma alta reserva de progenitores na medula ossea seja o maior determinante da resistencia a infeccao Abstract

Antioxidants, 2019

The aim of this study was to develop a phytocosmetic sunscreen emulsion with antioxidant effect, ... more The aim of this study was to develop a phytocosmetic sunscreen emulsion with antioxidant effect, containing a blend of flavonoid-enriched plant extracts. In vitro sun protection factor, antioxidant activity, skin irritation, photostability, cutaneous permeation, and retention of flavonoids were evaluated. Thermodynamically stable emulsions were obtained and tested for sensorial analysis after loading the blend of extracts. The selected emulsion was stable when stored at low temperatures (5 C), for which after 120 days the concentration of quercetin and rutin were above their limit of quantification, i.e., 2.8 ± 0.39 µg/mL and 30.39 ± 0.39 µg/mL, respectively. Spreadability, low rupture strength and adhesiveness were shown to be similar to a conventional topical product. Higher brittleness, pseudo-plastic, and viscoelastic behaviors were also recorded for the developed phytocosmetic sunscreen. The product presented a critical wavelength of 387.0 nm and ultraviolet rays A and B (UVA/U...

Supplemental Material, sj-docx-1-atl-10.1177_02611929211038652 for <i>Ex Vivo</i> Hum... more Supplemental Material, sj-docx-1-atl-10.1177_02611929211038652 for <i>Ex Vivo</i> Human Skin: An Alternative Test System for Skin Irritation and Corrosion Assays by Samara Eberlin, Gustavo Facchini, Gustavo Henrique da Silva, Samir Eberlin, Aline Rodrigues Bragatto, Ana Lúcia Tabarini Alves Pinheiro and Adriano da Silva Pinheiro in Alternatives to Laboratory Animals

Journal of The American Academy of Dermatology, 2020

Periorbital disobers (POD), cosmetic concern for large number of individuals, are characterized b... more Periorbital disobers (POD), cosmetic concern for large number of individuals, are characterized by an alteration in the color and shape of eyelids, manifested by hyperpigmentation (dark circles) and a safe and effective dermocosmetic product composed by concentration saponins and flavonoids, which corrects the multifonctional dissarrangement observed in POD physiopathology. Our results demonstrated that CEPPI reducted the levels of the main mediators of the inflammatory response, presented considerable lipolytic action, and promoted a stimuliting action on extracellular matrix protein synthesis. Clinical trials were also performed and demonstrated an improvement on dark circle tone, as measured by using a colorimetry assay, taken together, the results led us to conclude that CEPPL is an innovative and complete eyecare cosmetic indicated for dark circles and puffiness improvement.

Introducao: Introduction: Skin aging is a challenge to treat. Objectives: To evaluate the in vitr... more Introducao: Introduction: Skin aging is a challenge to treat. Objectives: To evaluate the in vitro and in vivo efficacy and clinical safety of a phytoestrogens-based cosmetic in the management of photoaging. Methods: The in vitro investigation was performed using the analysis of the genic expression of fibronectin and procollagen, evaluation of the immunomodulatory activity (proinflammatory and anti-inflammatory cytokines synthesis) and histochemistry and immunofluorescence analyses of the skin and the dermoepidermal junction. The in vivo investigation – performed in 76 women randomized into Group A (phytoestrogens complex cream and SPF 20 twice daily) or Group B (the same product plus a commercially available anti-aging cream applied overnight). The study lasted for 120 days, with physician- and patient-led evaluations, in addition to monthly ultrasound (20 MHz) and photographic analysis. Skin biopsies of the face were performed before and after treatment. Results: The study showed...

Desordens periorbitais (DPO) e um nome atribuido a um conjunto de disfuncoes esteticas caracteriz... more Desordens periorbitais (DPO) e um nome atribuido a um conjunto de disfuncoes esteticas caracterizadas por alteracoes na coloracao e na forma das palpebras. As principais manifestacoes observadas sao, hipercromia da regiao infraorbital, formacao de bolsas de gordura, de edema e de rugas finas. A hipercromia ocorre em funcao de um disturbio microvascular que pode gerar a formacao de edema e alteracao da tonalidade da pele. A formacao de bolsas de gordura e caracterizada por uma hipertrofia nos adipocitos devido ao acumulo de triacilglicerol intracelular. Pela acao deleteria da radiacao UV observa-se a degradacao do tecido conectivo, o que e refletido atraves do aparecimento de rugas ao redor dos olhos. Neste trabalho, avaliamos a possivel aplicacao da associacao dos extratos hidroglicolicos de Pfaffia paniculata e Ptychopetalum olacoides (AEHPPPO) na prevencao e tratamento da DPO. Os resultados clinicos obtidos demonstraram que AEHPPPO promoveu uma reducao da hipercromia, do edema e d...

People suffering from an ordinary acute cold consume so many handkerchiefs that the wiping action... more People suffering from an ordinary acute cold consume so many handkerchiefs that the wiping actions on their own increase the abrasive damage of the nasolabial zone, finally leading to a disturbed barrier function and inflamation. It seems that the quality of the material used for nose cleansing could play an important role and that innovative handkerchiefs would fulfil a preventive role in minimizing the damaging effect of the skin barrier function of the nasolabial zone during this conditions. The objective of this work was to evaluate the effects of a wet handkerchief (SKNW) on the skin barrier balance by measuring filaggrin and histamine using an experimental model of ex vivo native human skin model and the interference of this handkerchief in the skin microbiota through in vitro screening. SKNW showed an increase in the production of filaggrin and a reduction of histamine synthesis in human explants subjected to barrier disruption with 5% Sodium Lauryl Sulfate. Additionaly, SKNW...

Alternatives to Laboratory Animals

Native human skin has been reported in the literature as being an important experimental model fo... more Native human skin has been reported in the literature as being an important experimental model for studying skin biology. Studies performed by our group have shown that ex vivo skin, from elective plastic surgery, maintains the biological characteristics of native skin under specific culture conditions. As such, it might be a feasible model for the safety and efficacy testing of topical substances. While Brazil is at the forefront of global regulation implementation, Brazilian researchers are not always able to transfer certain widely used protocols to their laboratories, particularly protocols that involve the use of reconstructed tissues with limited viability, such as those for skin corrosion (OECD TG 431) and irritation testing (OECD TG 439). In this study, we investigated the applicability of the ex vivo skin model to the evaluation of irritation and corrosion potential of a number of proficiency substances described in TG 431 and TG 439. The skin fragments were standardised in...

Introducao: Hyperpigmentation is a common dermatological condition, usually associated with expos... more Introducao: Hyperpigmentation is a common dermatological condition, usually associated with exposure to the sun and relative difficulty of treatment. Pycnogenol® is an extract obtained from the French pine tree Pinus pinaster, with known antioxidant and anti-inflammatory activity, having been proven to inhibit melanin synthesis in previous studies. Objective: To evaluate the whitening activity of Pycnogenol® in an ex vivo experimental model after exposure to ultraviolet A and B, infrared-A radiations and visible light. Method: Human skin fragments obtained from elective plastic surgery were treated with Pycnogenol ® and then irradiated with ultraviolet A and B, and infrared-A radiations, and visible light, having been sent for histological evaluation of melanin pigmentation by the Fontana-Masson staining technique. Results: The histological evaluation demonstrated an increase in melanin deposition in all irradiated groups. However, fragments pre-incubated with Pycnogenol® showed a r...

Surgical and Cosmetic Dermatology, 2014

Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality... more Introduction: Obesity is a major health problem worldwide due to its high morbidity and mortality. Objective: To evaluate the reduction of abdominal fat in volunteers who underwent low-frequency diode laser therapy. Methods: A total of 60 volunteers (18 to 50 years of age, with a BMI of between 18.5 kg/m2 and 30 kg/m2, and who had abdominal fat), underwent a treatment performed twice a week for 31 days and were then evaluated. The abdominal circumference was evaluated in three locations, with photographic and ultrasound images taken of the studied area. Results: There was a reduction in the abdominal circumference measurements in the evaluated sites, with dermal compression and a significant reduction of hypodermis verified through ultrasound. Conclusion: The low-frequency diode laser therapy showed significant results in the reduction of localized fat and abdominal circumference measurements.

[

adjuvant to be used in dermocosmetic formulations. Clinical studies are in progress aiming to fur... more adjuvant to be used in dermocosmetic formulations. Clinical studies are in progress aiming to further investigate the protective effects of CGO in the skin [Maria Del Carmen Velazquez Pereda, Gustavo de Campos Dieamant, Samara Eberlin, Cecília Nogueira, Débora Colombi, Luiz Claudio Di Stasi and Mary Luci de Souza Queiroz (Department of Pharmacology/ Hemocenter, University of Campinas, Campinas, Brazil), J Cosmetic Dermatol, 2009, 8(1), 52-68].