Samuel Comar - Academia.edu (original) (raw)
Papers by Samuel Comar
Orientadora : Maria Suely Soares LeonartCo-orientadores : Aguinaldo José do Nascimento e Tomoe Na... more Orientadora : Maria Suely Soares LeonartCo-orientadores : Aguinaldo José do Nascimento e Tomoe NakashimaDissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Ciencias da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas.Resumo: O eritrócito humano maduro, quando submetido à sobrecarga oxidativa por condições patológicas do eritrócito ou por agentes oxidantes pode apresentar depleção de glutationa reduzida (GSH), um dos responsáveis pelo sistema de defesa antioxidante do eritrócito; oxidação da molécula de hemoglobina; e agregação de complexos de ferro junto à membrana. Tais conseqüências podem ocasionar anormalidades na membrana eritrocitária e hemólise. Este trabalho tem por objetivo, estudar a ação antioxidante dos flavonóides quercetina e rutina, e de extrato hidroalcoólico de Vitis virtifera (EHV), em eritrócitos humanos normais, submetidos à sobrecarga oxidativa, in vitro, induzida por agentes oxidantes como terc-butilhidroperóxido (TBH) ou cloridrato de fenil-hidrazina (CF). Coletou-se sangue venoso de 12 indivíduos com idades entre 18 e 40 anos e considerados saudáveis, em EDTAK3. Lavou-se os eritrócitos em tampão fosfato 28 mM, NaCl 123 mM, pH 7,4 três vezes por centrifugação a 1220 x g, ressuspendendo-os até volume globular de cerca de 40 %. Submeteu-se os eritrócitos, pré incubados ou não com quercetina 100 (xM, à ação oxidante de CF 0,5 mM durante 15 minutos e; pré-incubados ou não com quercetina, rutina ou extrato hidroalcoólico de Vitis virtifera, à ação oxidante de TBH (0,5 - 5 mM), durante 0 a 30 minutos. Realizou-se a determinação da concentração de GSH [BEUTLER, Red cell metabolism, 1984], da formação de metahemoglobina (MetHb) [EVELYN; MALLOY, JJBioLChem., 126: 655, 1938], modificado por BEUTLER (1995) e realizou-se a contagem de corpos de Heinz (CH) segundo técnica preconizada por BEUTLER et ai [J.Lab.&Clin.Méd., 45: 40, 1955], modificado por CLARO (2002). Observou-se uma diminuição da concentração de GSH (4,0-0,1 nmoles/ g Hb) proporcional ao aumento da concentração de TBH (0,5 - 1,0 mM). A formação de MetHb aumentou (8 - 35 %) assim como a de CH (2 -16 %), em função da concentração de TBH (0,5 - 5 mM). A incubação com quercetina 100 pM (10 - 30 minutos) preveniu parcialmente a formação de GSH (0,25 -1,3 junoles/g Hb) induzida por TBH 1 mM. Quercetina (2 - 20 JIM) preveniu parcialmente a formação de CH (7,5 - 6,7 %) induzida por TBH 3 mM. Em amostras oxidadas por CF 0,5 mM, a quercetina 10 - 120 pM preveniu parcialmente a formação de MetHb (4 - 3,6 %). A rutina 100 (xM preveniu parcialmente a depleção de GSH (0,4 pmoles/g Hb) por TBH (0 pmoles/g Hb). A rutina (40 - 140 jiM) preveniu parcialmente a formação de CH (7,6 - 4,9 %) induzida por TBH 3 mM (9,0 %). O EHV (0,1 mg/ml) preveniu parcialmente a depleção de GSH (4,4 ±0,1 jimoles/g Hb) induzida por TBH 0,5 mM enquanto que EHV (0,5 - 1,0 mg/ml) preveniu quase que na totalidade a depleção de GSH (7,4 ± 0,4 e 7,3 ±0,1 (imoles/ g Hb, respectivamente) induzida por TBH 0,5 mM. O EHV (1,0 - 5,0 mg/ml) preveniu parcialmente a formação de CH (6,4 - 4,0 %) induzida por TBH 3 mM. A associação entre quercetina 100 pM e mesilato de desferrioxamina 4 mM, inibiu parcialmente a formação de MetHb (30 ± 1,2 %) induzida por TBH 5 mM. Os resultados sugerem que, nas concentrações testadas, a quercetina é parcialmente eficaz contra a sobrecarga oxidativa promovida por CF. O estresse oxidativo promovido por TBH foi parcialmente prevenido na presença de quercetina, rutina ou EHV.Abstract: The mature human erythocytes, submitted to oxidative stress under pathological conditions or by oxidizer agents can present: depletion of reduced glutatione (GSH), responsible for the antioxidant defense system of the erythocytes, oxidation of the hemoglobin molecule, or aggregation of complexes of iron close to the membrane. These can cause abnormalities in the membrane and hemolysis. The aim of this work was to study the antioxidative action of the flavonoids quercetin and rutin, and of hydroalchoolic extract of Vitis vinifera (HEV), in human erythocytes submitted to the oxidative stress, induced by oxidizer agents fert-butylhydroperoxide (TBH) or phenylhydrazine hydrochloride (PH), in vitro. Venous blood was collected in EDTAK3 from 12 healthy individuals with ages between 18 and 40 years. The erythocytes was washed three times under centrifugation at 1220 x g, in 28 mM phosphate buffer, pH 7.4, and 123 mM NaCL, and ressuspended at a final globular volume of 40%. The erythocytes was then preincubated with 100 jiM quercetine and then submitted to the oxidizer action of 0.5 mM PH for 15 min and, preincubated with quercetin, rutin and hydroalchoolic extract of Vitis vinifera, to the oxidizer action of TBH (0.5 - 5 mM), for up to 30 min. The concentration of GSH was determinate [BEUTLER, Red cell metabolism, 1984], the methemoglobin (MetHb) was monitored [EVELYN; MALLOY, J.BioI.Chem., 126: 655, 1938], modified by BEUTLER et at. (1995) and tiie Heinz…
Revista Brasileira De Hematologia E Hemoterapia, Sep 1, 2014
Revista Brasileira De Hematologia E Hemoterapia, Oct 1, 2017
Background: There is great interest in reducing the number of automated complete blood counts req... more Background: There is great interest in reducing the number of automated complete blood counts requiring manual blood smear reviews without sacrificing the quality of patient care. This study was aimed at evaluating and establishing appropriate screening criteria for manual blood smear reviews to improve the performance in a hematology laboratory. Method: A total of 1977 consecutive samples from the daily workload were used to evaluate four sets of screening criteria for manual blood smear reviews to identify samples with positive smear findings. Three sets of screening criteria were arbitrarily proposed in this study: Group 1 (narrow ranges), Group 2 (intermediate ranges), and Group 3 (wide limits) and one set (Group 4) was adapted from the International Society for Laboratory Hematology. All samples were run on Sysmex hematology analyzers and were investigated using manual blood smear reviews. Diagnostic accuracy and agreement were performed for each set of screening criteria, including an investigation of microscopic review rate and efficiency. Results: The microscopic review rates for Groups 1, 2, 3 and 4 were 73.85%, 54.52%, 46.33% and 46.38%, respectively; the false-negative rates were 0.50%, 1.97%, 2.73% and 3.95%, respectively. The efficiency and negative predictive values of Group 3 were 73.04% and 4.91%, respectively. Conclusions: Group 3 had the best relationship between safety (false-negative rate: ≤3%) and efficiency to estimate the limits of automation in performing complete blood counts. This study strengthens the importance of establishing screening criteria for manual blood smear reviews, which account for the different contexts in which hematological determinations are performed. Each laboratory should optimize the screening criteria for manual blood smear reviews in order to maximize their efficiency and safety.
Revista Brasileira De Hematologia E Hemoterapia, May 1, 2014
Revista Brasileira De Hematologia E Hemoterapia, 2015
Rev. bras. anal. clin, 2011
Estudos de Biologia, Nov 27, 2011
Estudos de Biologia, 2008
The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid ... more The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid and its major function is the lubrification of the pleural surface helping the respiratory movements. Several primary and secondary pathologies affect the homeostasis of this liquid resulting in alterations quali and quantitative. The objective of the work is to propose a standardization of the cytological analysis of pleural fluid, increasing the reproductiveness and the precision of the results. The knowledge and execution of the techniques in the right way are essential for great results. Mistakes in any stage can result in alterations that can be significant and consequently it can damage the differentiation of transudate and exsudate, necessary for the diagnosis and therapeutic conduct. The standardization reduces the possibility of mistakes intra and inter-laboratories, increasing the reproductiveness, precision and exactness of the results, helping the doctor effectively in the determination of the etiology, diagnosis and treatment
Estudos de Biologia, Nov 27, 2009
Revista Brasileira de Análises Clínicas, 2021
Orientador: Prof. Dr. Ricardo PasquiniCoorientadora: Profa. Dra. Mariester MalvezziTese (doutorad... more Orientador: Prof. Dr. Ricardo PasquiniCoorientadora: Profa. Dra. Mariester MalvezziTese (doutorado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Medicina Interna. Defesa : Curitiba, 19/06/2015Inclui referências : f. 117-133Resumo: Há um grande interesse em laboratórios de hematologia em reduzir o número de hemogramas automatizados que exijam a análise microscópica, sem sacrificar a qualidade. Nesse sentido, o objetivo deste trabalho foi estabelecer e avaliar critérios para revisão microscópica de hemogramas automatizados (CRMHA) a fim de indicar os mais apropriados para o Laboratório de Hematologia do Hospital de Clínicas da UFPR (HC-UFPR). Em uma primeira etapa foram utilizadas e testadas 1977 amostras, empregando-se os critérios de triagem (CT) e os critérios de positividade em lâmina (CPL) da International Society for Laboratory Hematology (ISLH), cuja taxa de falsos negativos foi de 6,73% e não foram considerados seguros para indicar os hemogramas que poderiam ter sido liberados sem a revisão microscópica da lâmina. Para melhor atender as necessidades e peculiaridades desse Laboratório de Hematologia, em uma segunda etapa, novos CPL foram estabelecidos e analisados nas mesmas amostras, através de quatro conjuntos de CT, sendo um deles o adaptado da ISLH e outros três propostos nesse estudo, denominados de Estudo 1 (limites estreitos), Estudo 2 (limites intermediários) e Estudo 3 (limites amplos). Para cada conjunto de CT, as taxas de falsos positivos, falsos negativos (FN), verdadeiros positivos, verdadeiros negativos, sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo, taxa de revisões microscópicas e eficiência foram determinadas. Nos Estudos 1, 2, 3 e ISLH adaptados, considerando os CPL propostos neste estudo, as taxas de revisões microscópicas foram de 73,85%, 54,52%, 46,33% e 46,38%, respectivamente. Já as taxas de resultados FN foram de 0,50%, 1,97%, 2,73% e 3,95%, respectivamente. A eficiência e o valor preditivo negativo do Estudo 3 foram 73,04% e 94,91%, respectivamente. O Estudo 3 mostrou a melhor relação entre segurança e eficiência para estimar os limites da automação na realização de hemogramas. Cada laboratório deve avaliar os CRMHA baseados no Grupo Internacional de Consenso em Revisões Hematológicas da ISLH e, caso necessário, otimizá-los para maximizar sua eficiência e segurança. Esse estudo fortalece a necessidade de respeitar a heterogeneidade da população que utiliza o laboratório de modo que os CRMHA atendam às características de diferentes contextos nos quais se realizam determinações hematimétricas. Palavras-chave: Hematologia. Critérios de revisão de lâminas de hemograma. Analisadores hematológicos. Estudos de validação. AutomaçãoAbstract: To reduce costs and the time required to release full blood count results, there is great interest in reducing the number of automated blood count results requiring microscopic review without sacrificing quality in hematology laboratories. Towards to expand the knowledge on how to establish criteria for mandatory manual blood smear review we aimed to establish and evaluate criteria for manual blood smear review (CMBSR) in the Hematology Laboratory of the Hospital de Clínicas da Universidade Federal do Paraná in order to indicate the more appropriate CMBSR for this Hematology Laboratory. As a first step, a total of 1977 samples were utilized, which were tested using the adapted CMBSR of the International Society for Laboratory Hematology (ISLH), consisting of screening criteria adapted locally to capture samples with positive smear findings (PSF) defined by the ISLH. CMBSR adapted from ISLH shows 6.73% of FN and were unsafe to indicate blood counts that could have been released without manual peripheral blood smear review. In a second stage, we also evaluated on the same samples, four sets of screening criteria to capture samples of PSF proposed in this study, the set of screening criteria adapted ISLH and three other sets of screening criteria proposed by this study, called Study 1 (narrow ranges), Study 2 (intermediate ranges) and Study 3 (wide limits). For each set of screening criteria, false positive rates, false negatives (FN), true positives, true negatives, sensitivity, specificity, positive predictive value, negative predictive value, microscopic review rate and efficiency were determined. In Studies 1, 2, 3 and adapted from ISLH considering PSF proposed in this study, microscopic review rates were 73.85%, 54.52%, 46.33% and 46.38%, respectively. The FN rates were 0.50%, 1.97%, 2.73% and 3.95%, respectively. The efficiency and negative predictive value of Study 3 were 73.04% and 94.91%, respectively. Study 3 showed the best relationship between safety and efficiency to estimate the limits of automation in performing complete blood counts. Each laboratory should evaluate the criteria for manual blood smear review based on the International Consensus Group for…
Jornal Brasileiro De Patologia E Medicina Laboratorial, Aug 1, 2019
Introduction: Diagnosing infections in intensive care unit (ICU) patients is vital to provide app... more Introduction: Diagnosing infections in intensive care unit (ICU) patients is vital to provide appropriate therapies. Hematological analyzers perform automated immature granulocyte counts (IG) quickly and with no additional cost when compared to traditional microbiological cultures. Elevated IG is directly associated with infections and inflammation. Objectives: Evaluate IG as infection marker in adult inpatients at the ICU-Complexo Hospital de Clínicas da Universidade Federal do Paraná (CHC-UFPR), compared to cultures of biological materials (gold standard). Material and methods: Samples of 200 adult inpatients at CHC-UFPR ICU with suspected infection were used. Absolute (IG#) and relative (IG%) counts were performed on the Sysmex XN-3000. Cultures and blood cultures were performed either manually or on Bactec FX. Diagnostic accuracy and agreement for IG# and IG% were evaluated. Results: The reference intervals (RI) obtained for IG# and IG% were 0.06 × 10 3 /µl and 0.6%, respectively, with sensitivity for both of 74.4% and specificity of 25.3% for IG#, and 26.6% for IG%. The receiver operating characteristic (ROC) curve showed cutoff value of 0.33 × 10 3 /µl for IG#, sensitivity of 28%, specificity of 82.3%, and area under the curve (AUC) of 0.521. For IG%, cutoff value was 1.35%, sensitivity 44.6%, specificity 64.6%, and AUC 0.532. CV < 3% increased specificity to 88%. Conclusion: RI of IG% and IG# showed high sensitivity and are useful in screening for infection in ICU patients. The CVs demonstrated by the ROC curves showed high specificity and are helpful on the exclusion of sepsis diagnosis in ICU patients. IG was shown to be useful for screening and confirmation of infection in ICU patients.
Introduction: After treatment for Multiple Myeloma (MM), transformation into burkitt’s lymphoma (... more Introduction: After treatment for Multiple Myeloma (MM), transformation into burkitt’s lymphoma (LB) is uncommon, with rare cases reported. Objective: to report a rare case of LB secondary to MM, in a patient treated with the CyBorD protocol. Case report: We describe a case of a 37-year-old man, black, Senegalese and HIV positive, who in just over 2 months after the diagnosis of MM evolved to a medullary transformation that resulted in a B lymphoblastic lymphoma compatible with LB. Conclusion: This case report demonstrates the possibility of malignant transformation from MM to BL, thus encouraging future multicentric comparisons of similar cases in a broad effort to better define this pathological process.
Revista Brasileira de Análises Clínicas, 2021
Estudos De Biologia, 2008
The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid ... more The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid and its major function is the lubrification of the pleural surface helping the respiratory movements. Several primary and secondary pathologies affect the homeostasis of this liquid resulting in alterations quali and quantitative. The objective of the work is to propose a standardization of the cytological analysis of pleural fluid, increasing the reproductiveness and the precision of the results. The knowledge and execution of the techniques in the right way are essential for great results. Mistakes in any stage can result in alterations that can be significant and consequently it can damage the differentiation of transudate and exsudate, necessary for the diagnosis and therapeutic conduct. The standardization reduces the possibility of mistakes intra and inter-laboratories, increasing the reproductiveness, precision and exactness of the results, helping the doctor effectively in the determination of the etiology, diagnosis and treatment
Jornal Brasileiro de Patologia e Medicina Laboratorial, 2014
Introduction: The Sysmex ® XE-2100D is a multiparameter hematology analyzer designed for hematolo... more Introduction: The Sysmex ® XE-2100D is a multiparameter hematology analyzer designed for hematology testing in samples with ethylenediamine tetraacetic acid (EDTA). Objectives: Considering the importance of this hematology analyzer for clinical and laboratory practice, the objective of this study was to evaluate its analytical performance, comparing the obtained results with quality specifications described in literature. Material and method: In the evaluation of analytical performance, according to recommendations of the document H26-A2 of the Clinical and Laboratory Standards Institute (CLSI), intra-run imprecision, inter-run imprecision, linearity, carryover, autosampler evaluation, clinical sensitivity of the atypical lymphocytes flag (n = 400 samples) were included, as well as the comparison between automated and manual leukocyte differential count (n = 400 samples), based on an adaptation of the document H20-A2 of CLSI. Results: Repeatability, reproducibility, linearity and carryover were satisfactory according to the manufacturer's specifications. The clinical sensitivity of the atypical lymphocytes flag showed efficiency, sensitivity and specificity of 92.5%, 65.2% and 94.1% respectively. The correlation coefficients between the automated and manual differential counts of neutrophils, lymphocytes, monocytes, eosinophils and basophils were 0.991, 0.99, 0.872, 0.974 and 0.557, respectively. Conclusions: The results were in accordance with quality specifications described in literature, indicating reliability in Sysmex ® XE-2100D. This fact ensures certainty to both laboratory professionals and medical staff. We conclude that the Sysmex ® XE-2100D showed excellent analytical performance, and is useful to provide reliable hematology data.
Cell Biochemistry and Function, 2006
The oxidative action of 1 mmol l À1 phenylhydrazine hydrochloride (PH) was studied on human eryth... more The oxidative action of 1 mmol l À1 phenylhydrazine hydrochloride (PH) was studied on human erythrocytes treated with the antioxidants vitamin C (vit. C) and vitamin E (vit. E). The erythrocytes were resuspended in PBS to obtain 35% cell packed volume, and then submitted to the oxidative action of PH for 20 min, with or without previous incubation for 60 min with vit. C or vit. E. Heinz bodies and methemoglobin formation by PH were inhibited in the presence of vit. C. At the concentration of 90 mmol l À1 , vit. C, not only seemed to lose its antioxidant effect, but it also promoted an increase in methemoglobin formation. Vit. C (0.5-80 mmol l À1) did not protect against GSH depletion by PH. Vit. C alone produced insignificant hemolysis, but, in the presence of PH, the hemolysis indices were more accentuated. Heinz body formation by PH was inhibited in the presence of vit. E. Formation of methemoglobin induced by PH was decreased by vit. E (0.1-2 mmol l À1), although vit. E (3-80 mmol l À1) did not lower the concentration of methemoglobin and did not lead to the recovery of the GSH depleted by PH. The results obtained suggest that vit. C and vit. E contribute to the decrease in oxidative stress caused by PH.
Commercial control for blood cell values became available in the earlier sixties, but a lack of p... more Commercial control for blood cell values became available in the earlier sixties, but a lack of padronization for many measures involving cellular components still remains. Commercial cellular samples consist of human or animal blood components, altered for retarding degeneration. The names fixed, buffered, stabilized or preserved are commonly indicated without a clear description of the way they are processed. In previous works we developed samples that were adequate for quality control of red blood cell values for up to 100 days. The médium (CE medium) was composed of glucose, NaCl, sodium citrate, sodium monohydrogen phosphate, KCl, disodium EDTA, bovine serum albumin, chlormicetin, neomycin and cortisone plus red blood cells partially fixed with glutaraldehyde [Leonart et al., Rev.Bras.Anál.Clín. 21:111, 1989]. During the last decade, the use of additives for storage of platelet concentrates became frequent in clinical practice. In preliminary tests with the use of CE medium for...
Jornal Brasileiro de Patologia e Medicina Laboratorial
Hematology, Transfusion and Cell Therapy, 2021
Orientadora : Maria Suely Soares LeonartCo-orientadores : Aguinaldo José do Nascimento e Tomoe Na... more Orientadora : Maria Suely Soares LeonartCo-orientadores : Aguinaldo José do Nascimento e Tomoe NakashimaDissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Ciencias da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas.Resumo: O eritrócito humano maduro, quando submetido à sobrecarga oxidativa por condições patológicas do eritrócito ou por agentes oxidantes pode apresentar depleção de glutationa reduzida (GSH), um dos responsáveis pelo sistema de defesa antioxidante do eritrócito; oxidação da molécula de hemoglobina; e agregação de complexos de ferro junto à membrana. Tais conseqüências podem ocasionar anormalidades na membrana eritrocitária e hemólise. Este trabalho tem por objetivo, estudar a ação antioxidante dos flavonóides quercetina e rutina, e de extrato hidroalcoólico de Vitis virtifera (EHV), em eritrócitos humanos normais, submetidos à sobrecarga oxidativa, in vitro, induzida por agentes oxidantes como terc-butilhidroperóxido (TBH) ou cloridrato de fenil-hidrazina (CF). Coletou-se sangue venoso de 12 indivíduos com idades entre 18 e 40 anos e considerados saudáveis, em EDTAK3. Lavou-se os eritrócitos em tampão fosfato 28 mM, NaCl 123 mM, pH 7,4 três vezes por centrifugação a 1220 x g, ressuspendendo-os até volume globular de cerca de 40 %. Submeteu-se os eritrócitos, pré incubados ou não com quercetina 100 (xM, à ação oxidante de CF 0,5 mM durante 15 minutos e; pré-incubados ou não com quercetina, rutina ou extrato hidroalcoólico de Vitis virtifera, à ação oxidante de TBH (0,5 - 5 mM), durante 0 a 30 minutos. Realizou-se a determinação da concentração de GSH [BEUTLER, Red cell metabolism, 1984], da formação de metahemoglobina (MetHb) [EVELYN; MALLOY, JJBioLChem., 126: 655, 1938], modificado por BEUTLER (1995) e realizou-se a contagem de corpos de Heinz (CH) segundo técnica preconizada por BEUTLER et ai [J.Lab.&Clin.Méd., 45: 40, 1955], modificado por CLARO (2002). Observou-se uma diminuição da concentração de GSH (4,0-0,1 nmoles/ g Hb) proporcional ao aumento da concentração de TBH (0,5 - 1,0 mM). A formação de MetHb aumentou (8 - 35 %) assim como a de CH (2 -16 %), em função da concentração de TBH (0,5 - 5 mM). A incubação com quercetina 100 pM (10 - 30 minutos) preveniu parcialmente a formação de GSH (0,25 -1,3 junoles/g Hb) induzida por TBH 1 mM. Quercetina (2 - 20 JIM) preveniu parcialmente a formação de CH (7,5 - 6,7 %) induzida por TBH 3 mM. Em amostras oxidadas por CF 0,5 mM, a quercetina 10 - 120 pM preveniu parcialmente a formação de MetHb (4 - 3,6 %). A rutina 100 (xM preveniu parcialmente a depleção de GSH (0,4 pmoles/g Hb) por TBH (0 pmoles/g Hb). A rutina (40 - 140 jiM) preveniu parcialmente a formação de CH (7,6 - 4,9 %) induzida por TBH 3 mM (9,0 %). O EHV (0,1 mg/ml) preveniu parcialmente a depleção de GSH (4,4 ±0,1 jimoles/g Hb) induzida por TBH 0,5 mM enquanto que EHV (0,5 - 1,0 mg/ml) preveniu quase que na totalidade a depleção de GSH (7,4 ± 0,4 e 7,3 ±0,1 (imoles/ g Hb, respectivamente) induzida por TBH 0,5 mM. O EHV (1,0 - 5,0 mg/ml) preveniu parcialmente a formação de CH (6,4 - 4,0 %) induzida por TBH 3 mM. A associação entre quercetina 100 pM e mesilato de desferrioxamina 4 mM, inibiu parcialmente a formação de MetHb (30 ± 1,2 %) induzida por TBH 5 mM. Os resultados sugerem que, nas concentrações testadas, a quercetina é parcialmente eficaz contra a sobrecarga oxidativa promovida por CF. O estresse oxidativo promovido por TBH foi parcialmente prevenido na presença de quercetina, rutina ou EHV.Abstract: The mature human erythocytes, submitted to oxidative stress under pathological conditions or by oxidizer agents can present: depletion of reduced glutatione (GSH), responsible for the antioxidant defense system of the erythocytes, oxidation of the hemoglobin molecule, or aggregation of complexes of iron close to the membrane. These can cause abnormalities in the membrane and hemolysis. The aim of this work was to study the antioxidative action of the flavonoids quercetin and rutin, and of hydroalchoolic extract of Vitis vinifera (HEV), in human erythocytes submitted to the oxidative stress, induced by oxidizer agents fert-butylhydroperoxide (TBH) or phenylhydrazine hydrochloride (PH), in vitro. Venous blood was collected in EDTAK3 from 12 healthy individuals with ages between 18 and 40 years. The erythocytes was washed three times under centrifugation at 1220 x g, in 28 mM phosphate buffer, pH 7.4, and 123 mM NaCL, and ressuspended at a final globular volume of 40%. The erythocytes was then preincubated with 100 jiM quercetine and then submitted to the oxidizer action of 0.5 mM PH for 15 min and, preincubated with quercetin, rutin and hydroalchoolic extract of Vitis vinifera, to the oxidizer action of TBH (0.5 - 5 mM), for up to 30 min. The concentration of GSH was determinate [BEUTLER, Red cell metabolism, 1984], the methemoglobin (MetHb) was monitored [EVELYN; MALLOY, J.BioI.Chem., 126: 655, 1938], modified by BEUTLER et at. (1995) and tiie Heinz…
Revista Brasileira De Hematologia E Hemoterapia, Sep 1, 2014
Revista Brasileira De Hematologia E Hemoterapia, Oct 1, 2017
Background: There is great interest in reducing the number of automated complete blood counts req... more Background: There is great interest in reducing the number of automated complete blood counts requiring manual blood smear reviews without sacrificing the quality of patient care. This study was aimed at evaluating and establishing appropriate screening criteria for manual blood smear reviews to improve the performance in a hematology laboratory. Method: A total of 1977 consecutive samples from the daily workload were used to evaluate four sets of screening criteria for manual blood smear reviews to identify samples with positive smear findings. Three sets of screening criteria were arbitrarily proposed in this study: Group 1 (narrow ranges), Group 2 (intermediate ranges), and Group 3 (wide limits) and one set (Group 4) was adapted from the International Society for Laboratory Hematology. All samples were run on Sysmex hematology analyzers and were investigated using manual blood smear reviews. Diagnostic accuracy and agreement were performed for each set of screening criteria, including an investigation of microscopic review rate and efficiency. Results: The microscopic review rates for Groups 1, 2, 3 and 4 were 73.85%, 54.52%, 46.33% and 46.38%, respectively; the false-negative rates were 0.50%, 1.97%, 2.73% and 3.95%, respectively. The efficiency and negative predictive values of Group 3 were 73.04% and 4.91%, respectively. Conclusions: Group 3 had the best relationship between safety (false-negative rate: ≤3%) and efficiency to estimate the limits of automation in performing complete blood counts. This study strengthens the importance of establishing screening criteria for manual blood smear reviews, which account for the different contexts in which hematological determinations are performed. Each laboratory should optimize the screening criteria for manual blood smear reviews in order to maximize their efficiency and safety.
Revista Brasileira De Hematologia E Hemoterapia, May 1, 2014
Revista Brasileira De Hematologia E Hemoterapia, 2015
Rev. bras. anal. clin, 2011
Estudos de Biologia, Nov 27, 2011
Estudos de Biologia, 2008
The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid ... more The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid and its major function is the lubrification of the pleural surface helping the respiratory movements. Several primary and secondary pathologies affect the homeostasis of this liquid resulting in alterations quali and quantitative. The objective of the work is to propose a standardization of the cytological analysis of pleural fluid, increasing the reproductiveness and the precision of the results. The knowledge and execution of the techniques in the right way are essential for great results. Mistakes in any stage can result in alterations that can be significant and consequently it can damage the differentiation of transudate and exsudate, necessary for the diagnosis and therapeutic conduct. The standardization reduces the possibility of mistakes intra and inter-laboratories, increasing the reproductiveness, precision and exactness of the results, helping the doctor effectively in the determination of the etiology, diagnosis and treatment
Estudos de Biologia, Nov 27, 2009
Revista Brasileira de Análises Clínicas, 2021
Orientador: Prof. Dr. Ricardo PasquiniCoorientadora: Profa. Dra. Mariester MalvezziTese (doutorad... more Orientador: Prof. Dr. Ricardo PasquiniCoorientadora: Profa. Dra. Mariester MalvezziTese (doutorado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Medicina Interna. Defesa : Curitiba, 19/06/2015Inclui referências : f. 117-133Resumo: Há um grande interesse em laboratórios de hematologia em reduzir o número de hemogramas automatizados que exijam a análise microscópica, sem sacrificar a qualidade. Nesse sentido, o objetivo deste trabalho foi estabelecer e avaliar critérios para revisão microscópica de hemogramas automatizados (CRMHA) a fim de indicar os mais apropriados para o Laboratório de Hematologia do Hospital de Clínicas da UFPR (HC-UFPR). Em uma primeira etapa foram utilizadas e testadas 1977 amostras, empregando-se os critérios de triagem (CT) e os critérios de positividade em lâmina (CPL) da International Society for Laboratory Hematology (ISLH), cuja taxa de falsos negativos foi de 6,73% e não foram considerados seguros para indicar os hemogramas que poderiam ter sido liberados sem a revisão microscópica da lâmina. Para melhor atender as necessidades e peculiaridades desse Laboratório de Hematologia, em uma segunda etapa, novos CPL foram estabelecidos e analisados nas mesmas amostras, através de quatro conjuntos de CT, sendo um deles o adaptado da ISLH e outros três propostos nesse estudo, denominados de Estudo 1 (limites estreitos), Estudo 2 (limites intermediários) e Estudo 3 (limites amplos). Para cada conjunto de CT, as taxas de falsos positivos, falsos negativos (FN), verdadeiros positivos, verdadeiros negativos, sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo, taxa de revisões microscópicas e eficiência foram determinadas. Nos Estudos 1, 2, 3 e ISLH adaptados, considerando os CPL propostos neste estudo, as taxas de revisões microscópicas foram de 73,85%, 54,52%, 46,33% e 46,38%, respectivamente. Já as taxas de resultados FN foram de 0,50%, 1,97%, 2,73% e 3,95%, respectivamente. A eficiência e o valor preditivo negativo do Estudo 3 foram 73,04% e 94,91%, respectivamente. O Estudo 3 mostrou a melhor relação entre segurança e eficiência para estimar os limites da automação na realização de hemogramas. Cada laboratório deve avaliar os CRMHA baseados no Grupo Internacional de Consenso em Revisões Hematológicas da ISLH e, caso necessário, otimizá-los para maximizar sua eficiência e segurança. Esse estudo fortalece a necessidade de respeitar a heterogeneidade da população que utiliza o laboratório de modo que os CRMHA atendam às características de diferentes contextos nos quais se realizam determinações hematimétricas. Palavras-chave: Hematologia. Critérios de revisão de lâminas de hemograma. Analisadores hematológicos. Estudos de validação. AutomaçãoAbstract: To reduce costs and the time required to release full blood count results, there is great interest in reducing the number of automated blood count results requiring microscopic review without sacrificing quality in hematology laboratories. Towards to expand the knowledge on how to establish criteria for mandatory manual blood smear review we aimed to establish and evaluate criteria for manual blood smear review (CMBSR) in the Hematology Laboratory of the Hospital de Clínicas da Universidade Federal do Paraná in order to indicate the more appropriate CMBSR for this Hematology Laboratory. As a first step, a total of 1977 samples were utilized, which were tested using the adapted CMBSR of the International Society for Laboratory Hematology (ISLH), consisting of screening criteria adapted locally to capture samples with positive smear findings (PSF) defined by the ISLH. CMBSR adapted from ISLH shows 6.73% of FN and were unsafe to indicate blood counts that could have been released without manual peripheral blood smear review. In a second stage, we also evaluated on the same samples, four sets of screening criteria to capture samples of PSF proposed in this study, the set of screening criteria adapted ISLH and three other sets of screening criteria proposed by this study, called Study 1 (narrow ranges), Study 2 (intermediate ranges) and Study 3 (wide limits). For each set of screening criteria, false positive rates, false negatives (FN), true positives, true negatives, sensitivity, specificity, positive predictive value, negative predictive value, microscopic review rate and efficiency were determined. In Studies 1, 2, 3 and adapted from ISLH considering PSF proposed in this study, microscopic review rates were 73.85%, 54.52%, 46.33% and 46.38%, respectively. The FN rates were 0.50%, 1.97%, 2.73% and 3.95%, respectively. The efficiency and negative predictive value of Study 3 were 73.04% and 94.91%, respectively. Study 3 showed the best relationship between safety and efficiency to estimate the limits of automation in performing complete blood counts. Each laboratory should evaluate the criteria for manual blood smear review based on the International Consensus Group for…
Jornal Brasileiro De Patologia E Medicina Laboratorial, Aug 1, 2019
Introduction: Diagnosing infections in intensive care unit (ICU) patients is vital to provide app... more Introduction: Diagnosing infections in intensive care unit (ICU) patients is vital to provide appropriate therapies. Hematological analyzers perform automated immature granulocyte counts (IG) quickly and with no additional cost when compared to traditional microbiological cultures. Elevated IG is directly associated with infections and inflammation. Objectives: Evaluate IG as infection marker in adult inpatients at the ICU-Complexo Hospital de Clínicas da Universidade Federal do Paraná (CHC-UFPR), compared to cultures of biological materials (gold standard). Material and methods: Samples of 200 adult inpatients at CHC-UFPR ICU with suspected infection were used. Absolute (IG#) and relative (IG%) counts were performed on the Sysmex XN-3000. Cultures and blood cultures were performed either manually or on Bactec FX. Diagnostic accuracy and agreement for IG# and IG% were evaluated. Results: The reference intervals (RI) obtained for IG# and IG% were 0.06 × 10 3 /µl and 0.6%, respectively, with sensitivity for both of 74.4% and specificity of 25.3% for IG#, and 26.6% for IG%. The receiver operating characteristic (ROC) curve showed cutoff value of 0.33 × 10 3 /µl for IG#, sensitivity of 28%, specificity of 82.3%, and area under the curve (AUC) of 0.521. For IG%, cutoff value was 1.35%, sensitivity 44.6%, specificity 64.6%, and AUC 0.532. CV < 3% increased specificity to 88%. Conclusion: RI of IG% and IG# showed high sensitivity and are useful in screening for infection in ICU patients. The CVs demonstrated by the ROC curves showed high specificity and are helpful on the exclusion of sepsis diagnosis in ICU patients. IG was shown to be useful for screening and confirmation of infection in ICU patients.
Introduction: After treatment for Multiple Myeloma (MM), transformation into burkitt’s lymphoma (... more Introduction: After treatment for Multiple Myeloma (MM), transformation into burkitt’s lymphoma (LB) is uncommon, with rare cases reported. Objective: to report a rare case of LB secondary to MM, in a patient treated with the CyBorD protocol. Case report: We describe a case of a 37-year-old man, black, Senegalese and HIV positive, who in just over 2 months after the diagnosis of MM evolved to a medullary transformation that resulted in a B lymphoblastic lymphoma compatible with LB. Conclusion: This case report demonstrates the possibility of malignant transformation from MM to BL, thus encouraging future multicentric comparisons of similar cases in a broad effort to better define this pathological process.
Revista Brasileira de Análises Clínicas, 2021
Estudos De Biologia, 2008
The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid ... more The space between pleural sheets is filled out with an plasma ultrafiltered called pleural fluid and its major function is the lubrification of the pleural surface helping the respiratory movements. Several primary and secondary pathologies affect the homeostasis of this liquid resulting in alterations quali and quantitative. The objective of the work is to propose a standardization of the cytological analysis of pleural fluid, increasing the reproductiveness and the precision of the results. The knowledge and execution of the techniques in the right way are essential for great results. Mistakes in any stage can result in alterations that can be significant and consequently it can damage the differentiation of transudate and exsudate, necessary for the diagnosis and therapeutic conduct. The standardization reduces the possibility of mistakes intra and inter-laboratories, increasing the reproductiveness, precision and exactness of the results, helping the doctor effectively in the determination of the etiology, diagnosis and treatment
Jornal Brasileiro de Patologia e Medicina Laboratorial, 2014
Introduction: The Sysmex ® XE-2100D is a multiparameter hematology analyzer designed for hematolo... more Introduction: The Sysmex ® XE-2100D is a multiparameter hematology analyzer designed for hematology testing in samples with ethylenediamine tetraacetic acid (EDTA). Objectives: Considering the importance of this hematology analyzer for clinical and laboratory practice, the objective of this study was to evaluate its analytical performance, comparing the obtained results with quality specifications described in literature. Material and method: In the evaluation of analytical performance, according to recommendations of the document H26-A2 of the Clinical and Laboratory Standards Institute (CLSI), intra-run imprecision, inter-run imprecision, linearity, carryover, autosampler evaluation, clinical sensitivity of the atypical lymphocytes flag (n = 400 samples) were included, as well as the comparison between automated and manual leukocyte differential count (n = 400 samples), based on an adaptation of the document H20-A2 of CLSI. Results: Repeatability, reproducibility, linearity and carryover were satisfactory according to the manufacturer's specifications. The clinical sensitivity of the atypical lymphocytes flag showed efficiency, sensitivity and specificity of 92.5%, 65.2% and 94.1% respectively. The correlation coefficients between the automated and manual differential counts of neutrophils, lymphocytes, monocytes, eosinophils and basophils were 0.991, 0.99, 0.872, 0.974 and 0.557, respectively. Conclusions: The results were in accordance with quality specifications described in literature, indicating reliability in Sysmex ® XE-2100D. This fact ensures certainty to both laboratory professionals and medical staff. We conclude that the Sysmex ® XE-2100D showed excellent analytical performance, and is useful to provide reliable hematology data.
Cell Biochemistry and Function, 2006
The oxidative action of 1 mmol l À1 phenylhydrazine hydrochloride (PH) was studied on human eryth... more The oxidative action of 1 mmol l À1 phenylhydrazine hydrochloride (PH) was studied on human erythrocytes treated with the antioxidants vitamin C (vit. C) and vitamin E (vit. E). The erythrocytes were resuspended in PBS to obtain 35% cell packed volume, and then submitted to the oxidative action of PH for 20 min, with or without previous incubation for 60 min with vit. C or vit. E. Heinz bodies and methemoglobin formation by PH were inhibited in the presence of vit. C. At the concentration of 90 mmol l À1 , vit. C, not only seemed to lose its antioxidant effect, but it also promoted an increase in methemoglobin formation. Vit. C (0.5-80 mmol l À1) did not protect against GSH depletion by PH. Vit. C alone produced insignificant hemolysis, but, in the presence of PH, the hemolysis indices were more accentuated. Heinz body formation by PH was inhibited in the presence of vit. E. Formation of methemoglobin induced by PH was decreased by vit. E (0.1-2 mmol l À1), although vit. E (3-80 mmol l À1) did not lower the concentration of methemoglobin and did not lead to the recovery of the GSH depleted by PH. The results obtained suggest that vit. C and vit. E contribute to the decrease in oxidative stress caused by PH.
Commercial control for blood cell values became available in the earlier sixties, but a lack of p... more Commercial control for blood cell values became available in the earlier sixties, but a lack of padronization for many measures involving cellular components still remains. Commercial cellular samples consist of human or animal blood components, altered for retarding degeneration. The names fixed, buffered, stabilized or preserved are commonly indicated without a clear description of the way they are processed. In previous works we developed samples that were adequate for quality control of red blood cell values for up to 100 days. The médium (CE medium) was composed of glucose, NaCl, sodium citrate, sodium monohydrogen phosphate, KCl, disodium EDTA, bovine serum albumin, chlormicetin, neomycin and cortisone plus red blood cells partially fixed with glutaraldehyde [Leonart et al., Rev.Bras.Anál.Clín. 21:111, 1989]. During the last decade, the use of additives for storage of platelet concentrates became frequent in clinical practice. In preliminary tests with the use of CE medium for...
Jornal Brasileiro de Patologia e Medicina Laboratorial
Hematology, Transfusion and Cell Therapy, 2021