Samuele Voyron - Academia.edu (original) (raw)

Papers by Samuele Voyron

Fig. S2. Taxonomic (A) and trophic (B) kronas of detected fungal OTUs in the soil of Tobiotsuka k... more Fig. S2. Taxonomic (A) and trophic (B) kronas of detected fungal OTUs in the soil of Tobiotsuka kofun<br>

Genes

The aim of this study was to evaluate the impact of different moistening agents (RNase-free water... more The aim of this study was to evaluate the impact of different moistening agents (RNase-free water, absolute anhydrous ethanol, RNAlater®) applied to collection swabs on DNA/RNA retrieval and integrity for capillary electrophoresis applications (STR typing, cell type identification by mRNA profiling). Analyses were conducted on whole blood, luminol-treated diluted blood, saliva, semen, and mock skin stains. The effects of swab storage temperature and the time interval between sample collection and DNA/RNA extraction were also investigated. Water provided significantly higher DNA yields than ethanol in whole blood and semen samples, while ethanol and RNAlater® significantly outperformed water in skin samples, with full STR profiles obtained from over 98% of the skin samples collected with either ethanol or RNAlater®, compared to 71% of those collected with water. A significant difference in mRNA profiling success rates was observed in whole blood samples between swabs treated with eit...

Diversity, 2021

Hybridization can often lead to the formation of novel taxa which can have traits that resemble e... more Hybridization can often lead to the formation of novel taxa which can have traits that resemble either or both parental species. Determining the similarity of hybrid traits to parental taxa is particularly important in plant conservation, as hybrids that form between rare and common taxa may more closely resemble a rare parental species, thereby putting the rare parental taxon at further risk of extinction via increased backcrossing and introgression. We investigated the floral (morphological and chemical) traits and orchid mycorrhizal (OrM) fungal associations of the endangered orchid Orchis patens, its more common sister species O. provincialis, and their natural hybrid O. × fallax in natural sympatric populations. We found that both morphological and chemical floral traits of O. × fallax are shared by the parents but are more similar to O. patens than O. provincialis. OrM fungi were shared among all three taxa, indicating that the availability of OrM fungi should not represent a ...

Genome sequencing data revealed unexpected similarities among plant-interacting fungi belonging t... more Genome sequencing data revealed unexpected similarities among plant-interacting fungi belonging to different ecological guilds. In particular, the sequenced genomes of ericoid mycorrhizal fungi (ErMF) showed closer similarities with genomes of plant endophytes than with those of other mycorrhizal fungi. ErMF are typically associated with roots of plants in the Ericaceae, but it has never been investigated whether they also colonize other organs of their natural hosts. Here, we applied a metabarcoding approach to describe the fungal community associated with the different organs of Vaccinium myrtillus plants collected in the field. Taxa in the Helotiales and Sebacinales, known to include ErMF, characterize the root endosphere, together with Agaricales and Lecanoromycetes, while the stems were enriched in Agaricomycetes, Tremellomycetes and Pleosporales, the leaves were enriched in Sordariomycetes, Hysteriales, and the flowers were enriched in Dothideomycetes. Operational Taxonomic Un...

Changes in soil fungal community caused by land use have not been sufficiently studied in South-A... more Changes in soil fungal community caused by land use have not been sufficiently studied in South-American Andosols, considered globally as important food production areas. This study analyzed 26 soil samples of Andosols collected from locations devoted to conservation, agriculture and mining activities in the southeastern region of Antioquia, Colombia, to establish differences between fungal communities as indicators of the degree of soil perturbation. The study developed a novel heminested PCR with primers SSUmCf Mix, ITS4 and fITS7 to assess Arbuscular Mycorrhizal Fungi detection in a Illumina MiSeq metabarcoding on nuclear ribosomal ITS2 region. A non-metric multidimensional scaling allowed exploring driver factors of fungal community changes, while fitted Dirichlet-multinomial models and PERMANOVA tests allowed identifying the correlations between alpha diversity indexes and community dissimilarities, as well as the significance of land use effects on fungal community composition...

In my thesis I exemin the Jewish clothing rules that is a part of the Tzniut regulations. A bibli... more In my thesis I exemin the Jewish clothing rules that is a part of the Tzniut regulations. A biblical code of conduct that jewish people all over the world still followes. My research material has been internet sites that specialize in selling modest clothing to jewish women. I take a closer look at how these sites describe their clients and how they by doing that are taking a part in creating a specific jewish female identity. I also look at the impact this identity have on women's bodies. How it helps to dicipline women's bodies and keeps women dependent on males to defend them aginst other males. In my conclusion I also discuss the possibility to use modest clothing as a feminist tactic for women to take control over who has acces to their bodies. To do this I use theoris about performativity, clothing as communication and body politics, and I have choosen to use textual analysis as my method.

Methods in molecular biology, 2020

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ec... more Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ecological and economic impacts as they support plant nutrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by high-throughput sequencing through Illumina MiSeq platform of two DNA target regions: small subunit (SSU) and internal transcribed spacer (ITS). The protocol can apply to both soil and root AMF communities.

The isolation of arbuscular mycorrhizal fungi from different land use is the starting point for s... more The isolation of arbuscular mycorrhizal fungi from different land use is the starting point for selecting and producing inoculants. There are different techniques to isolate and produce large-scale arbuscular mycorrhizal fungi-based inoculum, being soil, inert substrate, and in vitro culture techniques among the most used by different biofertilizer producers. This chapter describes an active operating method to isolate and produce large-scale fungal inoculant in substrate-based manufacturing. In addition, critical parameters are presented for the optimal production of arbuscular mycorrhizal fungal inoculum. All the steps of the process are enlisted: from choosing the source of inoculum, its production, scaling, sustaining quality control, to shelf life.

Mycological Progress, 2020

Amongst the complicated group of Peziza with blue-violet-purplish hymenial surface, P. petersii, ... more Amongst the complicated group of Peziza with blue-violet-purplish hymenial surface, P. petersii, P. proteana f. proteana and f. sparassoides were studied using both morphological and molecular approaches (analysis of the nrITS and RPB2 sequences). The nrITS sequences were successfully obtained from the holotypes of Aleuria proteana, P. pseudoviolacea and Underwoodia campbellii. The epitype collection of P. petersii was designated, and its nrITS and RPB2 regions were sequenced. Our analyses showed that (i) P. proteana and P. petersii are a single species, and since P. petersii Berk. was published in 1875 and Aleuria proteana Boud. in 1899, the prioritary name is the former; (ii) P. proteana f. sparassoides (Boud.) Korf, the "cabbage-head fungus", is a distinct evolutionary line and is here recognised at species level using the oldest available epithet that can be combined in Peziza, Underwoodia campbellii Sacc. Consequently, Peziza campbellii nov. comb. is introduced. Keywords Pezizomycetes. Pezizales. Gyromitra phillipsii. Peziza lobulata. nrITS DNA and RPB2 sequences. Morphology. Taxonomy sparassoid/cabbage-shape of the ascomata [P. proteana f. sparassoides (Boud.) Korf] (Van Vooren 2003, 2017) or by the presence of a blue juice (P. saniosa Schrad.) (Donadini 1981; Medardi 2006; Van Vooren 2014). From the microscopic point of view, species identification is not an easy task, since there are species with both smooth and ornamented spores having similar dimensions and ornamentation pattern (Medardi 2006; Van Vooren 2014). P. petersii Berk. and P. proteana (Boud.) Seaver f. proteana show a carbonicolous habitat and very similar morphological features (Van Vooren 2003); hence, our idea of examining in depth their possible identity, also with molecular studies. In the extensive molecular phylogenetic works on Peziza

Frontiers in Ecology and Evolution, 2018

Journal of Cultural Heritage, 2018

Canadian Journal of Microbiology, 2017

Wood decomposition is a key step of the terrestrial carbon cycle and is of economic importance. I... more Wood decomposition is a key step of the terrestrial carbon cycle and is of economic importance. It is essentially a microbiological process performed by fungi and to an unknown extent by bacteria. To gain access to the genes expressed by the diverse microbial communities participating in wood decay, we developed an RNA extraction protocol from this recalcitrant material rich in polysaccharides and phenolic compounds. This protocol was implemented on 22 wood samples representing as many tree species from 11 plant families in the Angiosperms and Gymnosperms. RNA was successfully extracted from all samples and converted into cDNAs from which were amplified both fungal and bacterial protein coding genes, including genes encoding hydrolytic enzymes participating in lignocellulose hydrolysis. This protocol applicable to a wide range of decomposing wood types represents a first step towards a metatranscriptomic analysis of wood degradation under natural conditions.

The New phytologist, 2017

Mycorrhizal fungi are essential for the survival of orchid seedlings under natural conditions. Th... more Mycorrhizal fungi are essential for the survival of orchid seedlings under natural conditions. The distribution of these fungi in soil can constrain the establishment and resulting spatial arrangement of orchids at the local scale, but the actual extent of occurrence and spatial patterns of orchid mycorrhizal (OrM) fungi in soil remain largely unknown. We addressed the fine-scale spatial distribution of OrM fungi in two orchid-rich Mediterranean grasslands by means of high-throughput sequencing of fungal ITS2 amplicons, obtained from soil samples collected either directly beneath or at a distance from adult Anacamptis morio and Ophrys sphegodes plants. Like ectomycorrhizal and arbuscular mycobionts, OrM fungi (tulasnelloid, ceratobasidioid, sebacinoid and pezizoid fungi) exhibited significant horizontal spatial autocorrelation in soil. However, OrM fungal read numbers did not correlate with distance from adult orchid plants, and several of these fungi were extremely sporadic or unde...

Microbiology, 2017

⎯Microbially unexplored medicinal plants can have a genetically diverse microbial population with... more ⎯Microbially unexplored medicinal plants can have a genetically diverse microbial population with multi-functional plant growth promoting traits. In this aspect, 75 endophytic bacterial isolates with plant growth promoting traits were isolated from Withania coagulans Dunal and Olea ferruginea Royal. Many of these bacteria were able to solubilize phosphate, produce indole-3-acetic acid, ammonia as well as hydrogen cyanide, synthesize extracellular enzymes and show antagonistic activities against plant pathogenic fungi under in vitro conditions. These isolates were also characterized by morphological and biochemical analysis. Furthermore, four representative isolates with pronounced plant growth promoting activities were identified as Enterobacter cloacae, Enterobacter dissolvens, Enterobacter hormaechei and Cronobacter sakazakii by 16S rDNA sequencing analysis. This work for the first time, reported the isolation of endophytic bacteria, the novel association form selected plants, Withania coagulans and Olea ferruginea. The explored endophytes might have great potential in the field of biocontrol and plant growth promoting for sustainable agricultural practices.

The New phytologist, 2017

Orchids are highly dependent on their mycorrhizal fungal partners for nutrient supply, especially... more Orchids are highly dependent on their mycorrhizal fungal partners for nutrient supply, especially during early developmental stages. In addition to organic carbon, nitrogen (N) is probably a major nutrient transferred to the plant because orchid tissues are highly N-enriched. We know almost nothing about the N form preferentially transferred to the plant or about the key molecular determinants required for N uptake and transfer. We identified, in the genome of the orchid mycorrhizal fungus Tulasnella calospora, two functional ammonium transporters and several amino acid transporters but found no evidence of a nitrate assimilation system, in agreement with the N preference of the free-living mycelium grown on different N sources. Differential expression in symbiosis of a repertoire of fungal and plant genes involved in the transport and metabolism of N compounds suggested that organic N may be the main form transferred to the orchid host and that ammonium is taken up by the intracell...

New Biotechnology, 2013

(AAM) is copyrighted and published by Elsevier. It is posted here by agreement between Elsevier a... more (AAM) is copyrighted and published by Elsevier. It is posted here by agreement between Elsevier and the University of Turin. Changes resulting from the publishing process-such as editing, corrections, structural formatting, and other quality control mechanisms-may not be reflected in this version of the text. The definitive version of the text was subsequently published in NEW BIOTECHNOLOGY, 30 (6), 2013, 10.1016/j.nbt.2013.01.010. You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions: (1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license. (2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy.

Mycosphere, 2012

Hydnum ovoideisporum is a species of the Hydnum rufescens complex recently described from Spain, ... more Hydnum ovoideisporum is a species of the Hydnum rufescens complex recently described from Spain, and characterized by having a pileus with deep orange tones, ovoid to broadly ellipsoid basidiospores, and growing in Mediterranean areas on calcareous soil. In this paper, four South Italian collections found under Quercus and traditionally determined as H. rufescens, were analyzed on the basis of morphological and molecular (ITS) data; three of them were proven to be conspecific with H. ovoideisporum even though they differ from the type by globose to subglobose spores, and one has a smooth hymenophore. H. ovoideisporum f. depauperatum f. nov. is established for accommodating specimens with a smooth hymenophoral surface. Finally, H. magnorufescens sp. nov. is established for a collection with large basidiomes without strong orange-reddish tinges, and with globose to subglobose spores.

Fig. S2. Taxonomic (A) and trophic (B) kronas of detected fungal OTUs in the soil of Tobiotsuka k... more Fig. S2. Taxonomic (A) and trophic (B) kronas of detected fungal OTUs in the soil of Tobiotsuka kofun<br>

Genes

The aim of this study was to evaluate the impact of different moistening agents (RNase-free water... more The aim of this study was to evaluate the impact of different moistening agents (RNase-free water, absolute anhydrous ethanol, RNAlater®) applied to collection swabs on DNA/RNA retrieval and integrity for capillary electrophoresis applications (STR typing, cell type identification by mRNA profiling). Analyses were conducted on whole blood, luminol-treated diluted blood, saliva, semen, and mock skin stains. The effects of swab storage temperature and the time interval between sample collection and DNA/RNA extraction were also investigated. Water provided significantly higher DNA yields than ethanol in whole blood and semen samples, while ethanol and RNAlater® significantly outperformed water in skin samples, with full STR profiles obtained from over 98% of the skin samples collected with either ethanol or RNAlater®, compared to 71% of those collected with water. A significant difference in mRNA profiling success rates was observed in whole blood samples between swabs treated with eit...

Diversity, 2021

Hybridization can often lead to the formation of novel taxa which can have traits that resemble e... more Hybridization can often lead to the formation of novel taxa which can have traits that resemble either or both parental species. Determining the similarity of hybrid traits to parental taxa is particularly important in plant conservation, as hybrids that form between rare and common taxa may more closely resemble a rare parental species, thereby putting the rare parental taxon at further risk of extinction via increased backcrossing and introgression. We investigated the floral (morphological and chemical) traits and orchid mycorrhizal (OrM) fungal associations of the endangered orchid Orchis patens, its more common sister species O. provincialis, and their natural hybrid O. × fallax in natural sympatric populations. We found that both morphological and chemical floral traits of O. × fallax are shared by the parents but are more similar to O. patens than O. provincialis. OrM fungi were shared among all three taxa, indicating that the availability of OrM fungi should not represent a ...

Genome sequencing data revealed unexpected similarities among plant-interacting fungi belonging t... more Genome sequencing data revealed unexpected similarities among plant-interacting fungi belonging to different ecological guilds. In particular, the sequenced genomes of ericoid mycorrhizal fungi (ErMF) showed closer similarities with genomes of plant endophytes than with those of other mycorrhizal fungi. ErMF are typically associated with roots of plants in the Ericaceae, but it has never been investigated whether they also colonize other organs of their natural hosts. Here, we applied a metabarcoding approach to describe the fungal community associated with the different organs of Vaccinium myrtillus plants collected in the field. Taxa in the Helotiales and Sebacinales, known to include ErMF, characterize the root endosphere, together with Agaricales and Lecanoromycetes, while the stems were enriched in Agaricomycetes, Tremellomycetes and Pleosporales, the leaves were enriched in Sordariomycetes, Hysteriales, and the flowers were enriched in Dothideomycetes. Operational Taxonomic Un...

Changes in soil fungal community caused by land use have not been sufficiently studied in South-A... more Changes in soil fungal community caused by land use have not been sufficiently studied in South-American Andosols, considered globally as important food production areas. This study analyzed 26 soil samples of Andosols collected from locations devoted to conservation, agriculture and mining activities in the southeastern region of Antioquia, Colombia, to establish differences between fungal communities as indicators of the degree of soil perturbation. The study developed a novel heminested PCR with primers SSUmCf Mix, ITS4 and fITS7 to assess Arbuscular Mycorrhizal Fungi detection in a Illumina MiSeq metabarcoding on nuclear ribosomal ITS2 region. A non-metric multidimensional scaling allowed exploring driver factors of fungal community changes, while fitted Dirichlet-multinomial models and PERMANOVA tests allowed identifying the correlations between alpha diversity indexes and community dissimilarities, as well as the significance of land use effects on fungal community composition...

In my thesis I exemin the Jewish clothing rules that is a part of the Tzniut regulations. A bibli... more In my thesis I exemin the Jewish clothing rules that is a part of the Tzniut regulations. A biblical code of conduct that jewish people all over the world still followes. My research material has been internet sites that specialize in selling modest clothing to jewish women. I take a closer look at how these sites describe their clients and how they by doing that are taking a part in creating a specific jewish female identity. I also look at the impact this identity have on women's bodies. How it helps to dicipline women's bodies and keeps women dependent on males to defend them aginst other males. In my conclusion I also discuss the possibility to use modest clothing as a feminist tactic for women to take control over who has acces to their bodies. To do this I use theoris about performativity, clothing as communication and body politics, and I have choosen to use textual analysis as my method.

Methods in molecular biology, 2020

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ec... more Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ecological and economic impacts as they support plant nutrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by high-throughput sequencing through Illumina MiSeq platform of two DNA target regions: small subunit (SSU) and internal transcribed spacer (ITS). The protocol can apply to both soil and root AMF communities.

The isolation of arbuscular mycorrhizal fungi from different land use is the starting point for s... more The isolation of arbuscular mycorrhizal fungi from different land use is the starting point for selecting and producing inoculants. There are different techniques to isolate and produce large-scale arbuscular mycorrhizal fungi-based inoculum, being soil, inert substrate, and in vitro culture techniques among the most used by different biofertilizer producers. This chapter describes an active operating method to isolate and produce large-scale fungal inoculant in substrate-based manufacturing. In addition, critical parameters are presented for the optimal production of arbuscular mycorrhizal fungal inoculum. All the steps of the process are enlisted: from choosing the source of inoculum, its production, scaling, sustaining quality control, to shelf life.

Mycological Progress, 2020

Amongst the complicated group of Peziza with blue-violet-purplish hymenial surface, P. petersii, ... more Amongst the complicated group of Peziza with blue-violet-purplish hymenial surface, P. petersii, P. proteana f. proteana and f. sparassoides were studied using both morphological and molecular approaches (analysis of the nrITS and RPB2 sequences). The nrITS sequences were successfully obtained from the holotypes of Aleuria proteana, P. pseudoviolacea and Underwoodia campbellii. The epitype collection of P. petersii was designated, and its nrITS and RPB2 regions were sequenced. Our analyses showed that (i) P. proteana and P. petersii are a single species, and since P. petersii Berk. was published in 1875 and Aleuria proteana Boud. in 1899, the prioritary name is the former; (ii) P. proteana f. sparassoides (Boud.) Korf, the "cabbage-head fungus", is a distinct evolutionary line and is here recognised at species level using the oldest available epithet that can be combined in Peziza, Underwoodia campbellii Sacc. Consequently, Peziza campbellii nov. comb. is introduced. Keywords Pezizomycetes. Pezizales. Gyromitra phillipsii. Peziza lobulata. nrITS DNA and RPB2 sequences. Morphology. Taxonomy sparassoid/cabbage-shape of the ascomata [P. proteana f. sparassoides (Boud.) Korf] (Van Vooren 2003, 2017) or by the presence of a blue juice (P. saniosa Schrad.) (Donadini 1981; Medardi 2006; Van Vooren 2014). From the microscopic point of view, species identification is not an easy task, since there are species with both smooth and ornamented spores having similar dimensions and ornamentation pattern (Medardi 2006; Van Vooren 2014). P. petersii Berk. and P. proteana (Boud.) Seaver f. proteana show a carbonicolous habitat and very similar morphological features (Van Vooren 2003); hence, our idea of examining in depth their possible identity, also with molecular studies. In the extensive molecular phylogenetic works on Peziza

Frontiers in Ecology and Evolution, 2018

Journal of Cultural Heritage, 2018

Canadian Journal of Microbiology, 2017

Wood decomposition is a key step of the terrestrial carbon cycle and is of economic importance. I... more Wood decomposition is a key step of the terrestrial carbon cycle and is of economic importance. It is essentially a microbiological process performed by fungi and to an unknown extent by bacteria. To gain access to the genes expressed by the diverse microbial communities participating in wood decay, we developed an RNA extraction protocol from this recalcitrant material rich in polysaccharides and phenolic compounds. This protocol was implemented on 22 wood samples representing as many tree species from 11 plant families in the Angiosperms and Gymnosperms. RNA was successfully extracted from all samples and converted into cDNAs from which were amplified both fungal and bacterial protein coding genes, including genes encoding hydrolytic enzymes participating in lignocellulose hydrolysis. This protocol applicable to a wide range of decomposing wood types represents a first step towards a metatranscriptomic analysis of wood degradation under natural conditions.

The New phytologist, 2017

Mycorrhizal fungi are essential for the survival of orchid seedlings under natural conditions. Th... more Mycorrhizal fungi are essential for the survival of orchid seedlings under natural conditions. The distribution of these fungi in soil can constrain the establishment and resulting spatial arrangement of orchids at the local scale, but the actual extent of occurrence and spatial patterns of orchid mycorrhizal (OrM) fungi in soil remain largely unknown. We addressed the fine-scale spatial distribution of OrM fungi in two orchid-rich Mediterranean grasslands by means of high-throughput sequencing of fungal ITS2 amplicons, obtained from soil samples collected either directly beneath or at a distance from adult Anacamptis morio and Ophrys sphegodes plants. Like ectomycorrhizal and arbuscular mycobionts, OrM fungi (tulasnelloid, ceratobasidioid, sebacinoid and pezizoid fungi) exhibited significant horizontal spatial autocorrelation in soil. However, OrM fungal read numbers did not correlate with distance from adult orchid plants, and several of these fungi were extremely sporadic or unde...

Microbiology, 2017

⎯Microbially unexplored medicinal plants can have a genetically diverse microbial population with... more ⎯Microbially unexplored medicinal plants can have a genetically diverse microbial population with multi-functional plant growth promoting traits. In this aspect, 75 endophytic bacterial isolates with plant growth promoting traits were isolated from Withania coagulans Dunal and Olea ferruginea Royal. Many of these bacteria were able to solubilize phosphate, produce indole-3-acetic acid, ammonia as well as hydrogen cyanide, synthesize extracellular enzymes and show antagonistic activities against plant pathogenic fungi under in vitro conditions. These isolates were also characterized by morphological and biochemical analysis. Furthermore, four representative isolates with pronounced plant growth promoting activities were identified as Enterobacter cloacae, Enterobacter dissolvens, Enterobacter hormaechei and Cronobacter sakazakii by 16S rDNA sequencing analysis. This work for the first time, reported the isolation of endophytic bacteria, the novel association form selected plants, Withania coagulans and Olea ferruginea. The explored endophytes might have great potential in the field of biocontrol and plant growth promoting for sustainable agricultural practices.

The New phytologist, 2017

Orchids are highly dependent on their mycorrhizal fungal partners for nutrient supply, especially... more Orchids are highly dependent on their mycorrhizal fungal partners for nutrient supply, especially during early developmental stages. In addition to organic carbon, nitrogen (N) is probably a major nutrient transferred to the plant because orchid tissues are highly N-enriched. We know almost nothing about the N form preferentially transferred to the plant or about the key molecular determinants required for N uptake and transfer. We identified, in the genome of the orchid mycorrhizal fungus Tulasnella calospora, two functional ammonium transporters and several amino acid transporters but found no evidence of a nitrate assimilation system, in agreement with the N preference of the free-living mycelium grown on different N sources. Differential expression in symbiosis of a repertoire of fungal and plant genes involved in the transport and metabolism of N compounds suggested that organic N may be the main form transferred to the orchid host and that ammonium is taken up by the intracell...

New Biotechnology, 2013

(AAM) is copyrighted and published by Elsevier. It is posted here by agreement between Elsevier a... more (AAM) is copyrighted and published by Elsevier. It is posted here by agreement between Elsevier and the University of Turin. Changes resulting from the publishing process-such as editing, corrections, structural formatting, and other quality control mechanisms-may not be reflected in this version of the text. The definitive version of the text was subsequently published in NEW BIOTECHNOLOGY, 30 (6), 2013, 10.1016/j.nbt.2013.01.010. You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions: (1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license. (2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy.

Mycosphere, 2012

Hydnum ovoideisporum is a species of the Hydnum rufescens complex recently described from Spain, ... more Hydnum ovoideisporum is a species of the Hydnum rufescens complex recently described from Spain, and characterized by having a pileus with deep orange tones, ovoid to broadly ellipsoid basidiospores, and growing in Mediterranean areas on calcareous soil. In this paper, four South Italian collections found under Quercus and traditionally determined as H. rufescens, were analyzed on the basis of morphological and molecular (ITS) data; three of them were proven to be conspecific with H. ovoideisporum even though they differ from the type by globose to subglobose spores, and one has a smooth hymenophore. H. ovoideisporum f. depauperatum f. nov. is established for accommodating specimens with a smooth hymenophoral surface. Finally, H. magnorufescens sp. nov. is established for a collection with large basidiomes without strong orange-reddish tinges, and with globose to subglobose spores.