Sandor Boldogh - Academia.edu (original) (raw)

Papers by Sandor Boldogh

Hystrix-italian Journal of Mammalogy, Jul 3, 2017

Hibernating mammals arouse periodically from their torpor under the influence of an unknown mecha... more Hibernating mammals arouse periodically from their torpor under the influence of an unknown mechanism to perform activities necessary for the correct functioning of metabolism. Our model species, the Mediterranean horseshoe bat (Rhinolophus euryale Blasius, 1853), wakes up during the winter and produces both typical consumptive as well as non-consumptive faeces (produced after no feeding activity). The aim of this study was to characterize the composition of the latter droppings of R. euryale in comparison to summer droppings to better understand the processes involved in such arousals from winter hibernation. The non-consumptive samples were morphologically similar and consisted of mucous material on the outside and a homogeneous mass inside. The internal homogenous mass inside the faeces was composed of organic as well as inorganic material, consisting of phosphate, calcium carbonate, quartz particles, and clay. We also confirmed the overall presence of faecal spherulites, calcite crystals and siliceous needles. The faeces contained no cells or if some, merely fragments of them. In contrast, summer faeces were composed of insect fragments; i.e. they were typically consumptive. In addition, we found no trace of insect-prey DNA in the winter droppings, which contained bat DNA instead. We also found peptides belonging to Mammalia as well as to other craniate and eukaryotes, but Arthropoda peptides occurred in only a rudimental occurrence. We found Bacteria peptides as well. Internal parasites were also visually retrieved. The high concentration of inorganic material and virtual lack of prey observed in the non-consumptive faeces indicate that drinking as well as direct sediment consumption occur inside the cave environment during the hibernation period. We conclude that winter arousals are unlikely to be aimed at gaining energy through foraging but most likely allow regulating water balance by active drinking.

The bat fauna of the Gömör-Torna Karst region has been studied for several decades, in par ti cu ... more The bat fauna of the Gömör-Torna Karst region has been studied for several decades, in par ti cu lar that of the Aggtelek Karst (Hungary). The earliest research was mainly conducted in the well-known Baradla cave system, whilst other sites in the region were somewhat neglected. At the end of the 1980s a new research programme started, when studies on house-dwelling bat species and a comparative analysis of results was systematically carried out. These results played an important role in the conservation strategies for the protection of roosts (e.g. in churches). Some of these roosts were of international im por tan ce. During the third period of research, a survey of man-made caverns and of forest-dwelling bat po pu la ti ons began. This research revealed that 26 bat species inhabited the administrative area of the Aggtelek National Park (24 species in the proper area of the national park). These fi gures prove that there is a very rich bat fauna in terms of both species and their populations. The latest faunistical studies and the pro te cti on of these populations have both signifi cantly improved our knowledge on the distribution and occur ren ce of several species. At the same time, it became necessary to reassess all previous data.

Ornis Hungarica, 2016

The Corncrake is a strictly protected species in Hungary and a qualifying species of many N

Journal of General Virology, Oct 1, 2020

Polyomaviruses (PyVs) are small, circular dsDNA viruses carried by diverse vertebrates, including... more Polyomaviruses (PyVs) are small, circular dsDNA viruses carried by diverse vertebrates, including bats. Although previous studies have reported several horseshoe bat PyVs collected in Zambia and China, it is still unclear how PyVs evolved in this group of widely dispersed mammals. Horseshoe bats (genus Rhinolophus) are distributed across the Old World and are natural reservoirs of numerous pathogenic viruses. Herein, non-invasive bat samples from European horseshoe bat species were collected in Hungary for PyV identification and novel PyVs with complete genomes were successfully recovered from two different European horseshoe bat species. Genomic and phylogenetic analysis of the Hungarian horseshoe bat PyVs supported their classification into the genera Alphapolyomavirus and Betapolyomavirus. Notably, despite the significant geographical distances between the corresponding sampling locations, Hungarian PyVs exhibited high genetic relatedness with previously described Zambian and Chinese horseshoe bat PyVs, and phylogenetically clustered with these viruses in each PyV genus. Correlation and virus–host relationship analysis suggested that these PyVs co-diverged with their European, African and Asian horseshoe bat hosts distributed on different continents during their evolutionary history. Additionally, assessment of selective pressures over the major capsid protein (VP1) of horseshoe bat PyVs showed sites under positive selection located in motifs exposed to the exterior of the capsid. In summary, our findings revealed a pattern of stable intrahost divergence of horseshoe bat PyVs with their mammalian hosts on the African and Eurasian continents over evolutionary time.

Acta Veterinaria Hungarica, Oct 6, 2022

Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis in humans, dogs, ca... more Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis in humans, dogs, cats, horses and tick-borne fever in ruminants. In Europe, its main vector is the tick species Ixodes ricinus. In this study, spleen and liver samples, as well as ticks from 18 wild-living mammals (belonging to seven species) were analysed for the presence of A. phagocytophilum with molecular methods. The zoonotic ecotype-I of A. phagocytophilum was identified in a European wildcat (Felis silvestris) and its tick, a European pine marten (Martes martes) and a Eurasian red squirrel (Sciurus vulgaris). All PCR-positive samples were collected in 2019 and originated in the same geographic area. These results indicate that taxonomically diverse mammalian species can maintain the local enzootic cycle of the same genotype of A. phagocytophilum. To the best of our knowledge, this is the first report of the zoonotic variant of A. phagocytophilum in the wildcat and in the European pine marten in a broad geographical context, as well as in the red squirrel in Hungary. Since all these host species are well known for their urban and peri-urban presence, the results of this study verify their role in the synanthropic enzootic cycle of granulocytic anaplasmosis and tick-borne fever.

Pathogens, Apr 28, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of General Virology, Apr 1, 2015

Bats are important reservoirs of many viruses with zoonotic potential worldwide, including Europe... more Bats are important reservoirs of many viruses with zoonotic potential worldwide, including Europe. Among bat viruses, members of the Picornaviridae family remain a neglected group. We performed viral metagenomic analyses on Miniopterus schreibersii bat faecal samples, collected in Hungary in 2013. In the present study we report the first molecular data and genomic characterization of a novel picornavirus from the bat species M. schreibersii in Europe. Based on phylogenetic analyses, the novel bat picornaviruses unambiguously belong to the Mischivirus genus and were highly divergent from other bat-derived picornaviruses of the Sapelovirus genus. Although the Hungarian viruses were most closely related to Mischivirus A, they formed a separate monophyletic branch within the genus. Recently, we have initiated a survey to describe bat-borne viruses in the European bat fauna, with particular emphasis on RNA viruses, to uncover the occurrence of viruses posing a zoonotic threat in Hungary. In addition, we The GenBank/EMBL/DDBJ accession numbers for the BatPV sequences were KP054273-KP054278.

Social Science Research Network, 2023

International Journal of Infectious Diseases, 2022

Hystrix-italian Journal of Mammalogy, 2009

We collected data on the reproduction of Nyctalus noctula in Hungary by mist-netting and monitori... more We collected data on the reproduction of Nyctalus noctula in Hungary by mist-netting and monitoring bat boxes. The capture or observation of juveniles and lactating females were considered evidence of breeding. A total of 1413 N. noctula were mist-netted in four study areas, corresponding to 24 new breeding locations for Hungary. In southern Hungary, one breeding colony with 20-25 individuals was observed in a bat box in a protected park, while another was found in the hollow of a white poplar. Twenty lactating females and 3 juveniles were mist-netted in the Zemplen Mountains. Our data document the regular breeding of the species in Hungary, the breeding area being wider than previously reported. Further breeding colonies are likely to be located in the floodplain forests of some main rivers, such as the Tisza and the Danube. Riassunto Sempre piu a sud: nuovi dati sull’areale riproduttivo di Nyctalus noctula (Schreber, 1774) in Europa centrale Tramite cattura con reti e il monitorag...

Antonie van Leeuwenhoek, 2020

Increasing amount of data attest that (in the context of vector-borne infections) birds are not o... more Increasing amount of data attest that (in the context of vector-borne infections) birds are not only important as hosts of blood-sucking arthropod vectors, but also as reservoirs of vector-borne pathogens. From 2015 to 2019 cadavers of 100 birds (from 45 species, nine orders) were collected in Hungary, and their organs were screened for DNA from a broad range of vector-borne bacteria with PCR and sequencing. Molecular analyses revealed the presence of Anaplasmataceae, and sequencing identified bacteria closely related to Neorickettsia helminthoeca and Ehrlichia chaffeensis in a Eurasian teal (Anas crecca) and a song thrush (Turdus philomelos), respectively. All samples were PCR negative for rickettsiae, borreliae, Francisella and Coxiella spp., as well as for piroplasms. To our knowledge, this is the first report of a Neorickettsia and an Ehrlichia sp., which belong to the phylogenetic groups of N. helminthoeca and E. chaffeensis, respectively, from Europe. The potential presence of...

Systematic and Applied Acarology, 2019

Bats are well adapted to inhabit human settlements and are suitable reservoirs of a high number o... more Bats are well adapted to inhabit human settlements and are suitable reservoirs of a high number of vector-borne pathogens with veterinary-medical importance. Owing to these eco-epidemiological traits, the importance of studying bat ectoparasites is increasingly recognized. However, relevant molecular-phylogenetic data are missing from several countries of southern Asia, including Pakistan. In this study 11 ectoparasites, collected from bats in northern Pakistan, were analyzed morphologically and/or molecularly, phylogenetically from a taxonomic point of view. In addition, soft ticks were screened for pathogen DNA. Three mesostigmatid mite species were identified: Steatonyssus occidentalis evansi Micherdziński, 1980 and Ancystropus taprobanius Turk, 1950 from Rousettus leschenaultii (Desmarest 1820) and two specimens of Spinturnix americanus (Banks 1902) from Pipistrellus cf. javanicus (Gray 1838). Six soft tick (Carios vespertilionis Latreille, 1802) larvae were also removed from Sc...

International Journal of Infectious Diseases, 2019

Emerging Microbes & Infections, 2018

Infection, Genetics and Evolution, 2016

Emerging viral diseases represent an ongoing challenge for globalized world and bats constitute a... more Emerging viral diseases represent an ongoing challenge for globalized world and bats constitute an immense, partially explored, reservoir of potentially zoonotic viruses. Caliciviruses are important human and animal pathogens and, as observed for human noroviruses, they may impact on human health on a global scale. By screening fecal samples of bats in Hungary, calicivirus RNA was identified in the samples of Myotis daubentonii and Eptesicus serotinus bats. In order to characterize more in detail the bat caliciviruses, large portions of the genome sequence of the viruses were determined. Phylogenetic analyses and molecular modeling identified firmly the two viruses as candidate members within the Caliciviridae family, with one calicivirus strain resembling members of the Sapovirus genus and the other bat calicivirus being more related to porcine caliciviruses of the proposed genus Valovirus. This data serves the effort for detecting reservoir hosts for potential emerging viruses and recognize important evolutionary relationships.

Parasites & Vectors, 2019

Background: Despite the increasingly recognized eco-epidemiological significance of bats, data fr... more Background: Despite the increasingly recognized eco-epidemiological significance of bats, data from molecular analyses of vector-borne bacteria in bat ectoparasites are lacking from several regions of the Old and New Worlds. Methods: During this study, six species of ticks (630 specimens) were collected from bats in Hungary,

bioRxiv (Cold Spring Harbor Laboratory), Aug 10, 2021

Genome Announcements, Jun 30, 2016

We report the complete genome sequence and genetic characterization of a novel strain of Providen... more We report the complete genome sequence and genetic characterization of a novel strain of Providence virus, detected in Barbastella barbastellus bat guano, collected in Hungary in 2014. Our data may facilitate the understanding of the evolutionary processes of this unique viral family of Carmotetraviridae.

Vector-borne and Zoonotic Diseases, Dec 1, 2014

Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasi... more Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasing scientific interest. To start gaining information about bat-transmitted viruses in Hungary, we tested multiple bat species for several virus groups between 2012 and 2013. Materials and Methods: Fecal samples were collected from bats across Hungary. We performed group-specific RT-PCR screening for astro-, calici-, corona-, lyssa-, othoreo-, paramyxo-, and rotaviruses. Positive samples were selected and sequenced for further phylogenetic analyses. Results: A total of 447 fecal samples, representing 24 European bat species were tested. Novel strains of astroviruses, coronaviruses, and caliciviruses were detected and analyzed phylogenetically. Out of the 447 tested samples, 40 (9%) bats were positive for at least one virus. Bat-transmitted astroviruses (BtAstV) were detected in eight species with a 6.93% detection rate (95% confidence interval [CI] 4.854, 9.571). Coronaviruses (BtCoV) were detected in seven bat species with a detection rate of 1.79% (95% CI 0.849, 3.348), whereas novel caliciviruses (BtCalV) were detected in three bat species with a detection rate of 0.67% (95% CI 0.189, 1.780). Phylogenetic analyses revealed a great diversity among astrovirus strains, whereas the Hungarian BtCoV strains clustered together with both alpha-and betacoronavirus strains from other European countries. One of the most intriguing findings of our investigation is the discovery of novel BtCalVs in Europe. The Hungarian BtCalV did not cluster with any of the calcivirus genera identified in the family so far. Conclusions: We have successfully confirmed BtCoVs in numerous bat species. Furthermore, we have described new bat species harboring BtAstVs in Europe and found new species of CalVs. Further long-term investigations involving more species are needed in the Central European region for a better understanding on the host specificity, seasonality, phylogenetic relationships, and the possible zoonotic potential of these newly described viruses.

Hystrix-italian Journal of Mammalogy, Jul 3, 2017

Hibernating mammals arouse periodically from their torpor under the influence of an unknown mecha... more Hibernating mammals arouse periodically from their torpor under the influence of an unknown mechanism to perform activities necessary for the correct functioning of metabolism. Our model species, the Mediterranean horseshoe bat (Rhinolophus euryale Blasius, 1853), wakes up during the winter and produces both typical consumptive as well as non-consumptive faeces (produced after no feeding activity). The aim of this study was to characterize the composition of the latter droppings of R. euryale in comparison to summer droppings to better understand the processes involved in such arousals from winter hibernation. The non-consumptive samples were morphologically similar and consisted of mucous material on the outside and a homogeneous mass inside. The internal homogenous mass inside the faeces was composed of organic as well as inorganic material, consisting of phosphate, calcium carbonate, quartz particles, and clay. We also confirmed the overall presence of faecal spherulites, calcite crystals and siliceous needles. The faeces contained no cells or if some, merely fragments of them. In contrast, summer faeces were composed of insect fragments; i.e. they were typically consumptive. In addition, we found no trace of insect-prey DNA in the winter droppings, which contained bat DNA instead. We also found peptides belonging to Mammalia as well as to other craniate and eukaryotes, but Arthropoda peptides occurred in only a rudimental occurrence. We found Bacteria peptides as well. Internal parasites were also visually retrieved. The high concentration of inorganic material and virtual lack of prey observed in the non-consumptive faeces indicate that drinking as well as direct sediment consumption occur inside the cave environment during the hibernation period. We conclude that winter arousals are unlikely to be aimed at gaining energy through foraging but most likely allow regulating water balance by active drinking.

The bat fauna of the Gömör-Torna Karst region has been studied for several decades, in par ti cu ... more The bat fauna of the Gömör-Torna Karst region has been studied for several decades, in par ti cu lar that of the Aggtelek Karst (Hungary). The earliest research was mainly conducted in the well-known Baradla cave system, whilst other sites in the region were somewhat neglected. At the end of the 1980s a new research programme started, when studies on house-dwelling bat species and a comparative analysis of results was systematically carried out. These results played an important role in the conservation strategies for the protection of roosts (e.g. in churches). Some of these roosts were of international im por tan ce. During the third period of research, a survey of man-made caverns and of forest-dwelling bat po pu la ti ons began. This research revealed that 26 bat species inhabited the administrative area of the Aggtelek National Park (24 species in the proper area of the national park). These fi gures prove that there is a very rich bat fauna in terms of both species and their populations. The latest faunistical studies and the pro te cti on of these populations have both signifi cantly improved our knowledge on the distribution and occur ren ce of several species. At the same time, it became necessary to reassess all previous data.

Ornis Hungarica, 2016

The Corncrake is a strictly protected species in Hungary and a qualifying species of many N

Journal of General Virology, Oct 1, 2020

Polyomaviruses (PyVs) are small, circular dsDNA viruses carried by diverse vertebrates, including... more Polyomaviruses (PyVs) are small, circular dsDNA viruses carried by diverse vertebrates, including bats. Although previous studies have reported several horseshoe bat PyVs collected in Zambia and China, it is still unclear how PyVs evolved in this group of widely dispersed mammals. Horseshoe bats (genus Rhinolophus) are distributed across the Old World and are natural reservoirs of numerous pathogenic viruses. Herein, non-invasive bat samples from European horseshoe bat species were collected in Hungary for PyV identification and novel PyVs with complete genomes were successfully recovered from two different European horseshoe bat species. Genomic and phylogenetic analysis of the Hungarian horseshoe bat PyVs supported their classification into the genera Alphapolyomavirus and Betapolyomavirus. Notably, despite the significant geographical distances between the corresponding sampling locations, Hungarian PyVs exhibited high genetic relatedness with previously described Zambian and Chinese horseshoe bat PyVs, and phylogenetically clustered with these viruses in each PyV genus. Correlation and virus–host relationship analysis suggested that these PyVs co-diverged with their European, African and Asian horseshoe bat hosts distributed on different continents during their evolutionary history. Additionally, assessment of selective pressures over the major capsid protein (VP1) of horseshoe bat PyVs showed sites under positive selection located in motifs exposed to the exterior of the capsid. In summary, our findings revealed a pattern of stable intrahost divergence of horseshoe bat PyVs with their mammalian hosts on the African and Eurasian continents over evolutionary time.

Acta Veterinaria Hungarica, Oct 6, 2022

Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis in humans, dogs, ca... more Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis in humans, dogs, cats, horses and tick-borne fever in ruminants. In Europe, its main vector is the tick species Ixodes ricinus. In this study, spleen and liver samples, as well as ticks from 18 wild-living mammals (belonging to seven species) were analysed for the presence of A. phagocytophilum with molecular methods. The zoonotic ecotype-I of A. phagocytophilum was identified in a European wildcat (Felis silvestris) and its tick, a European pine marten (Martes martes) and a Eurasian red squirrel (Sciurus vulgaris). All PCR-positive samples were collected in 2019 and originated in the same geographic area. These results indicate that taxonomically diverse mammalian species can maintain the local enzootic cycle of the same genotype of A. phagocytophilum. To the best of our knowledge, this is the first report of the zoonotic variant of A. phagocytophilum in the wildcat and in the European pine marten in a broad geographical context, as well as in the red squirrel in Hungary. Since all these host species are well known for their urban and peri-urban presence, the results of this study verify their role in the synanthropic enzootic cycle of granulocytic anaplasmosis and tick-borne fever.

Pathogens, Apr 28, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of General Virology, Apr 1, 2015

Bats are important reservoirs of many viruses with zoonotic potential worldwide, including Europe... more Bats are important reservoirs of many viruses with zoonotic potential worldwide, including Europe. Among bat viruses, members of the Picornaviridae family remain a neglected group. We performed viral metagenomic analyses on Miniopterus schreibersii bat faecal samples, collected in Hungary in 2013. In the present study we report the first molecular data and genomic characterization of a novel picornavirus from the bat species M. schreibersii in Europe. Based on phylogenetic analyses, the novel bat picornaviruses unambiguously belong to the Mischivirus genus and were highly divergent from other bat-derived picornaviruses of the Sapelovirus genus. Although the Hungarian viruses were most closely related to Mischivirus A, they formed a separate monophyletic branch within the genus. Recently, we have initiated a survey to describe bat-borne viruses in the European bat fauna, with particular emphasis on RNA viruses, to uncover the occurrence of viruses posing a zoonotic threat in Hungary. In addition, we The GenBank/EMBL/DDBJ accession numbers for the BatPV sequences were KP054273-KP054278.

Social Science Research Network, 2023

International Journal of Infectious Diseases, 2022

Hystrix-italian Journal of Mammalogy, 2009

We collected data on the reproduction of Nyctalus noctula in Hungary by mist-netting and monitori... more We collected data on the reproduction of Nyctalus noctula in Hungary by mist-netting and monitoring bat boxes. The capture or observation of juveniles and lactating females were considered evidence of breeding. A total of 1413 N. noctula were mist-netted in four study areas, corresponding to 24 new breeding locations for Hungary. In southern Hungary, one breeding colony with 20-25 individuals was observed in a bat box in a protected park, while another was found in the hollow of a white poplar. Twenty lactating females and 3 juveniles were mist-netted in the Zemplen Mountains. Our data document the regular breeding of the species in Hungary, the breeding area being wider than previously reported. Further breeding colonies are likely to be located in the floodplain forests of some main rivers, such as the Tisza and the Danube. Riassunto Sempre piu a sud: nuovi dati sull’areale riproduttivo di Nyctalus noctula (Schreber, 1774) in Europa centrale Tramite cattura con reti e il monitorag...

Antonie van Leeuwenhoek, 2020

Increasing amount of data attest that (in the context of vector-borne infections) birds are not o... more Increasing amount of data attest that (in the context of vector-borne infections) birds are not only important as hosts of blood-sucking arthropod vectors, but also as reservoirs of vector-borne pathogens. From 2015 to 2019 cadavers of 100 birds (from 45 species, nine orders) were collected in Hungary, and their organs were screened for DNA from a broad range of vector-borne bacteria with PCR and sequencing. Molecular analyses revealed the presence of Anaplasmataceae, and sequencing identified bacteria closely related to Neorickettsia helminthoeca and Ehrlichia chaffeensis in a Eurasian teal (Anas crecca) and a song thrush (Turdus philomelos), respectively. All samples were PCR negative for rickettsiae, borreliae, Francisella and Coxiella spp., as well as for piroplasms. To our knowledge, this is the first report of a Neorickettsia and an Ehrlichia sp., which belong to the phylogenetic groups of N. helminthoeca and E. chaffeensis, respectively, from Europe. The potential presence of...

Systematic and Applied Acarology, 2019

Bats are well adapted to inhabit human settlements and are suitable reservoirs of a high number o... more Bats are well adapted to inhabit human settlements and are suitable reservoirs of a high number of vector-borne pathogens with veterinary-medical importance. Owing to these eco-epidemiological traits, the importance of studying bat ectoparasites is increasingly recognized. However, relevant molecular-phylogenetic data are missing from several countries of southern Asia, including Pakistan. In this study 11 ectoparasites, collected from bats in northern Pakistan, were analyzed morphologically and/or molecularly, phylogenetically from a taxonomic point of view. In addition, soft ticks were screened for pathogen DNA. Three mesostigmatid mite species were identified: Steatonyssus occidentalis evansi Micherdziński, 1980 and Ancystropus taprobanius Turk, 1950 from Rousettus leschenaultii (Desmarest 1820) and two specimens of Spinturnix americanus (Banks 1902) from Pipistrellus cf. javanicus (Gray 1838). Six soft tick (Carios vespertilionis Latreille, 1802) larvae were also removed from Sc...

International Journal of Infectious Diseases, 2019

Emerging Microbes & Infections, 2018

Infection, Genetics and Evolution, 2016

Emerging viral diseases represent an ongoing challenge for globalized world and bats constitute a... more Emerging viral diseases represent an ongoing challenge for globalized world and bats constitute an immense, partially explored, reservoir of potentially zoonotic viruses. Caliciviruses are important human and animal pathogens and, as observed for human noroviruses, they may impact on human health on a global scale. By screening fecal samples of bats in Hungary, calicivirus RNA was identified in the samples of Myotis daubentonii and Eptesicus serotinus bats. In order to characterize more in detail the bat caliciviruses, large portions of the genome sequence of the viruses were determined. Phylogenetic analyses and molecular modeling identified firmly the two viruses as candidate members within the Caliciviridae family, with one calicivirus strain resembling members of the Sapovirus genus and the other bat calicivirus being more related to porcine caliciviruses of the proposed genus Valovirus. This data serves the effort for detecting reservoir hosts for potential emerging viruses and recognize important evolutionary relationships.

Parasites & Vectors, 2019

Background: Despite the increasingly recognized eco-epidemiological significance of bats, data fr... more Background: Despite the increasingly recognized eco-epidemiological significance of bats, data from molecular analyses of vector-borne bacteria in bat ectoparasites are lacking from several regions of the Old and New Worlds. Methods: During this study, six species of ticks (630 specimens) were collected from bats in Hungary,

bioRxiv (Cold Spring Harbor Laboratory), Aug 10, 2021

Genome Announcements, Jun 30, 2016

We report the complete genome sequence and genetic characterization of a novel strain of Providen... more We report the complete genome sequence and genetic characterization of a novel strain of Providence virus, detected in Barbastella barbastellus bat guano, collected in Hungary in 2014. Our data may facilitate the understanding of the evolutionary processes of this unique viral family of Carmotetraviridae.

Vector-borne and Zoonotic Diseases, Dec 1, 2014

Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasi... more Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasing scientific interest. To start gaining information about bat-transmitted viruses in Hungary, we tested multiple bat species for several virus groups between 2012 and 2013. Materials and Methods: Fecal samples were collected from bats across Hungary. We performed group-specific RT-PCR screening for astro-, calici-, corona-, lyssa-, othoreo-, paramyxo-, and rotaviruses. Positive samples were selected and sequenced for further phylogenetic analyses. Results: A total of 447 fecal samples, representing 24 European bat species were tested. Novel strains of astroviruses, coronaviruses, and caliciviruses were detected and analyzed phylogenetically. Out of the 447 tested samples, 40 (9%) bats were positive for at least one virus. Bat-transmitted astroviruses (BtAstV) were detected in eight species with a 6.93% detection rate (95% confidence interval [CI] 4.854, 9.571). Coronaviruses (BtCoV) were detected in seven bat species with a detection rate of 1.79% (95% CI 0.849, 3.348), whereas novel caliciviruses (BtCalV) were detected in three bat species with a detection rate of 0.67% (95% CI 0.189, 1.780). Phylogenetic analyses revealed a great diversity among astrovirus strains, whereas the Hungarian BtCoV strains clustered together with both alpha-and betacoronavirus strains from other European countries. One of the most intriguing findings of our investigation is the discovery of novel BtCalVs in Europe. The Hungarian BtCalV did not cluster with any of the calcivirus genera identified in the family so far. Conclusions: We have successfully confirmed BtCoVs in numerous bat species. Furthermore, we have described new bat species harboring BtAstVs in Europe and found new species of CalVs. Further long-term investigations involving more species are needed in the Central European region for a better understanding on the host specificity, seasonality, phylogenetic relationships, and the possible zoonotic potential of these newly described viruses.