Sania Begum - Academia.edu (original) (raw)

Papers by Sania Begum

Indian Journal of Genetics and Plant Breeding, 2010

Polish Journal of Environmental Studies, Feb 6, 2024

The study was designed to evaluate the genetic background of indigenous germplasm by using simple... more The study was designed to evaluate the genetic background of indigenous germplasm by using simple sequence repeats (SSRs) gene markers for the selection of elite lines with multiple gene combinations that can be used in breeding programs for wheat improvement against rust disease. The principal component analysis (PCA) was constructed to estimate the genetic difference between the accessions and revealed an 85.3% variation. A high level of association was found between markers XWMC170 /XGWM608, XGWM44 a /XGWM44 b , XGDM111 a /XGWM129, XWMC765 a /XWMC765 b , and GWM148 a /XWMC765 b while low level of correlation was observed between the markers CSLV34b and CSLV34 a i.e-0.688 followed by GWM148 a and XGWM129 (-0.633). Cluster analysis revealed three

Applied Ecology and Environmental Research, 2022

Begum et al.: Genetic transformation of the EPSPS herbicide resistance gene in Agrobacterium medi... more Begum et al.: Genetic transformation of the EPSPS herbicide resistance gene in Agrobacterium mediated peanut (Arachis hypogaea L.

Molecules, Sep 21, 2022

Synthesis of Silver Nanoparticles Using Catharanthus roseus and Its Cytotoxicity Effect on Vero C... more Synthesis of Silver Nanoparticles Using Catharanthus roseus and Its Cytotoxicity Effect on Vero Cell Lines.

Molecular Biology Reports, May 30, 2023

Background: Soil salinity has been affecting wheat production worldwide over past few decades. Ev... more Background: Soil salinity has been affecting wheat production worldwide over past few decades. Evaluation of wheat genotypes for salinity tolerance at germination and vegetative growth level is crucial. Marker assisted selection is a technique used extensively for choosing salt-tolerant genotypes from breeding populations to introduce novel genes. Methods and materials: The current study's main goal was to discover salt-stress resistant genes; genetic divergence and genome-wide connection by using recently designed candidate gene-based simplesequence-repeat markers (cg-SSRs). The phenotypic connection of morphological features during the germination growth stage i.e., germination period, root length/weight and shoot length/weight, and vegetative growth stages i.e., root length/weight and shoot length/weight were tested in a group of 50 wheat genotypes. Signi cant difference was observed in germination rate, root length and weight among control and saline treatments. Conclusion: Total 30 SSR markers were utilized to test salinity resistance genes in wheat genotypes. Three (10%) of which were monomorphic, one (3.34%) showed no result, and the other 26 (86%) were polymorphic. Using 30 polymorphic markers discovered total 37 alleles. The polymorphic information content (PIC), quanti es each SSR locus capacity to discriminate between wheat, varied from 0.00 to 0.38 with an average of 0.19. Association analysis revealed that 26 primers were associated with morphological features, 03 with root length and the remaining 23 with germination. Utilizing morphological data, stress tolerance index (STI) was designed concluding that Auqab-2000, Margala-99 and Ufaq showed better resistance against salinity among other wheat genotypes. Cluster analysis demonstrated that wheat genotypes have vast genetic variability.

Current Stem Cell Research & Therapy

: Stem cells, which were initially identified in the 1900s, are distinct cells with the potential... more : Stem cells, which were initially identified in the 1900s, are distinct cells with the potential to replenish themselves as well as differentiate into specialised cells with certain forms and functions. Cancer stem cells play a significant role in the growth and recurrence of the tumours and, similar to normal stem cells, are capable of proliferating and differentiating. Traditional cancer treatments are ineffective against cancer stem cells, which leads to tumour regrowth. Cancer stem cells are thought to emerge as a result of epithelial-to-mesenchymal transition pathways. Brain, prostate, pancreatic, blood, ovarian, lung, liver, melanomas, AML, and breast cancer stem cells are among the most prevalent cancer forms. This review aims to comprehend the possibility of using specific forms of nanotechnology to replace cancer stem cells. In terms of nanotechnology, magnetic nanoparticles can deliver medications, especially to the target region without harming healthy cells, and they are biocompatible. In order to kill glioma cancer stem cells, the gold nanoparticles bond with DNA and function as radio sensitizers. In contrast, liposomes can circulate and traverse biological membranes and exhibit high therapeutic efficacy, precise targeting, and better drug release. Similar to carbon nanotubes, grapheme, and grapheme oxide, these substances can be delivered specifically when utilized in photothermal therapy. Recent treatments including signaling pathways and indicators targeted by nanoparticles are being researched. Future research in nanotechnology aims to develop more effective and targeted medicinal approaches. The results of the current investigation also showed that this technology's utilization will improve medical therapy and treatment.

Agronomy

Banana is one of the major cash and fruit crops of Pakistan. The lack of information concerning g... more Banana is one of the major cash and fruit crops of Pakistan. The lack of information concerning genetic diversity and purity within locally cultivated banana varieties is a major bottleneck in improving its genetics. Due to the existence of a narrow genetic background, it’s quite important to find genomic variations in banana varieties. DNA marker-based techniques have been used to effectively characterize banana varieties. In the current study, Inter Simple Sequence Repeat (ISSR) markers were used to characterize banana cultivars and to assess the genetic diversity of 14 local banana varieties grown in Pakistan. Out of the 45 primers used, 40 primers revealed reproducible results and produced 121 polymorphic bands, which contributed a ratio of 47.87 polymorphism. The ISSR UBC-835 and UBC-834 possessed the highest PIC ranged between (86–88%) in banana varieties, while the lowest PIC (46%) was detected in the case of UBC−857 marker with (100–1500 bp) PCR product size. Pairwise Jaccar...

Acta Scientiarum Polonorum Hortorum Cultus

Brassica species commonly known as rapeseed are the second largest oil yielding crop of Pakistan ... more Brassica species commonly known as rapeseed are the second largest oil yielding crop of Pakistan and belongs to the family Brassicaceae. This scientific investigation was carried out to explore the genetic diversity of Brassica rapa from Azad Jammu and Kashmir (AJK) Pakistan. Seeds of Brassica rapa L. genotypes of Azad Jammu and Kashmir were collected from Plant Genetic Research Institute (PGRI), National Agricultural Research Centre (NARC), Islamabad Pakistan. CTAB method was used for the extraction of DNA from leaf samples. SSR and ISSR markers were used to explore the genetic diversity and cluster analysis was used for data analysis. Out of 6 SSR markers, Ra2E11 and Na10-E02 showed maximum genetic diversity while Ra2-E07, Ra2-D04, and Na10-D03 showed moderate diversity while Ra2-E01 showed minimum genetic diversity. Among the elected genotypes 026503 (Bhimber), 026514 (Kotli), 026510 (Kotli) and 026539 (Bagh) showed maximum diversity with 6 SSR markers. From 10 ISSR markers ISSR ...

Pakistan Journal of Botany

Pakistan Journal of Botany, 2018

In the computer system masquerader is an outside or a corporate executive user who take the privi... more In the computer system masquerader is an outside or a corporate executive user who take the privileges or authentication to act as associate legal user in system. Such user takes identity of a legal user to performing some illegal or a fraud activity. Till currently varied researchers has been accomplish for detecting masqueraders. Semi-global alignment approach is one Best algorithm however it doesn't reach to that much of accuracy and performance that is needed by high scale industries. To overcome such a disadvantage we discover a new technique referred to as data Driven Semi-Global Alignment Approach (DDSGA). In this algorithm we try to enhance security of system by adopting rating parameter for an every user. DDSGA also minimize the alignment overhead. The process of Detection and change is completed in a parallel manner experimental results that show that DDSGA achieves a high hit ratio of 88.4 % with a low false positive rate of 1.7 percent.

This research was conducted at The University of Agriculture, Peshawar during soybean cropping se... more This research was conducted at The University of Agriculture, Peshawar during soybean cropping seasons, 2011 and 2012. The plant material comprised of parental lines of US germplasm (US), landraces from Kurram Agency (KA) and lines collected from Plant Genetic Resource Institute (PG) NARC Islamabad soybean genotypes. These lines were studied for genetic variability, heritability and genotype x year interaction (GEI), using RCB design with two replications. Data was recorded on leaf area, days to flowering, and maturity, plant height, 100 seed weight and seed yield plant. Analysis of variance showed significant differences among the genotypes for 100 seed weight, DPI, days to flowering, plant height, maturity, seed yield plant, and leaf area. GEI was significant only for plant height, seed yield plant and leaf area. Based on mean performance, local genotype KA1-2 initiated flowers early (62 days) and whereas parental lines of US08 produced pods early (87days) in first growing season ...

Microsatellite markers or simple sequence repeats (SSR) are believed to be a reliable tool in the... more Microsatellite markers or simple sequence repeats (SSR) are believed to be a reliable tool in the assessment of genetic variation within genomes and are therefore, widely used in population studies. In the present study, microsatellite markers were employed to confirm the process of introgression in F2hybrids derived from interspecific crosses of Oleiferous brassica species viz., B. napus (AACC genomeused as female parent) crossed with each B. juncea (AABB) and B. rapa (AA). A total of 31 SSR primers were utilized and 99 alleles were detected, with 69 (69.7%) being polymorphic. Polymorphism information content of the primer sets ranged from 0.24 to 0.75. In population of B. napus x B. junceae, pair wise similarity indices ranged from 4.4 to 9.2and from 4.2 to 8.2 in F2 hybrids ofB. napus x B. rapa. The deviation of Introgressed hybrids towards female parent in both populations revealed that C genome has played a vital role in the introgression process of brassica species under study...

Acta Scientiarum Polonorum Hortorum Cultus, 2020

Brassica rapa is an important crop of Azad Jammu and Kashmir which is a major source of vegetable... more Brassica rapa is an important crop of Azad Jammu and Kashmir which is a major source of vegetable and oil. The study was carried out to evaluate the genetic diversity among Brassica rapa germplasm grown in Azad Jammu and Kashmir based on their morphological characters and yield. The present study was carried out in the field of National Institute of Genomics and Advanced Biotechnology (NIGAB), NARC Islamabad from October 2017 to March 2018. The study showed great diversity among the germplasm for days to germination, primary branches, secondary branches, maturity, plant height, number of silique, seeds per silique, yield per plant and 1000 seed weight. The genotypes 026509, 026514 and 026548 showed maximum yield and other better morphological responses, so these genotypes should be used in future to enhance the production of this crop. ...

Pakistan Journal of Weed Science Research, 2015

Ephedra procera Fisch & Mey is an important wild medicinal, evergreen dioecious shrub found fragm... more Ephedra procera Fisch & Mey is an important wild medicinal, evergreen dioecious shrub found fragmentally at high altitudes of Balochistan, Pakistan. Published data regarding the E. procera is limited in the area under discussion. Therefore study was initiated to determine genetic diversity of this species from three different altitudes of Quetta valley, Balochistan through genetic polymorphism (by estimating genetic distances). The study was carried out during 2012-2014 using different molecular markers, ten-mer Random amplified polymorphic DNA (RAPD) and eighteen-merInter-Simple Sequence Repeat (ISSR). Out of total sampled plants, nine were selected for this study. Out of 35RAPD and 5 ISSRs, 6 RAPD and 3 ISSRs were able to characterize the studied genotypes. A total of 92 DNA fragments were amplified and 57 (61.95%) were polymorphic with an average of 10.2 amplified per primer. Unweighted pair group method of arithmetic means (UPGMA) method was used for cluster analysis indicating ...

Four wild medicinal and aromatic plants were studied for their allelopathic potential against Con... more Four wild medicinal and aromatic plants were studied for their allelopathic potential against Convolvulus arvensis. The wild plants were collected from different elevation zones of Quetta, Balochistan. Two species of genus Sophora viz. Sophora mollis (Royel) Baker. and S. alopecuroides L. were collected from Sra Ghurgai (Takatu mountain Range) and University of Balochistan Quetta campus, respectively. While Perovskia abrotanoides Karel. was collected from SraGhurgai and Zarghoon region of different elevation zones and Peganum harmala L. was collected from SraGhurgai (Takatu Mountain Range) and University of Balochistan Quetta Campus. Phytotoxic effect of the four plants was studied at various grades of aqueous extracts i.e 4 and 32% (w/v). To check germination impact and seedling growth of weed C. arvensis L. water-soluble phenolic content and its impact as inhibitory substance was also investigated along with the impact of altitudinal gradient on phenolic concentration and its effe...

Juniperusexcelsa (Cupressaceae) is an evergreen tree and the second most diverse group of the con... more Juniperusexcelsa (Cupressaceae) is an evergreen tree and the second most diverse group of the conifers distributed abundantly in high elevation zones of Balochistan. Genetic and phytochemical variations in three naturally occurring populations of J.excelsa were analysed. Genetic variability was assessed by different molecular markers (RAPD, ISSR and URP) with an objective to use genetic diversity as a key to conserve the taxon which is also known as living fossil as dominated in Mesozoic era. Genetic diversity was assessed by polymorphic bands to generate a dendrogram based on UPGMA. Using tested markers, 116 bands were amplified out of which 67 bands were polymorphic with an average value of 8.37 (57%) bands per primer. Based on data, a cluster dendrogram was prepared that exhibited the mean genetic similarity matrix as 0.57 and two major clusters diverge at 0.49. The genetic similarity coefficient among all accessions ranged from 0.35 to 0.90. In phytochemical analysis, total phen...

Berberis is an important genus of Berberidaceae. This genus is of great economic and medicinal va... more Berberis is an important genus of Berberidaceae. This genus is of great economic and medicinal value. Berberis baluchistanica (endemic to Balochistan) selected for this study is used traditionally by local communities to cure different ailments such as internal injuries and infection of human and livestock, thus over collection of this significant taxa leads to its population depletion. Genetic diversity of B. baluchistanica has been studied for the first time from different sites of Zarghoon and Sra Ghurgai (Takatu-mountain range) of Balochistan from 2012-2014 by using Simple Sequence Repeats (SSR). Out of total sampled genotypes six were selected for this study. The morphological and molecular variation was estimated by Numerical Taxonomy and Multivariate Analysis System (NTSYS pc). DNA Amplification of all genotypes was studied by using SSR markers. Out of seven primer pairs of SSR markers, four amplified twenty-seven allelic variants with twenty-two polymorphic bands (81%) with ...

JPMA. The Journal of the Pakistan Medical Association, 2018

Adverse drug reactions (ADRs) are very common with anticancer drugs. The objectives of the survey... more Adverse drug reactions (ADRs) are very common with anticancer drugs. The objectives of the survey are to evaluate ADRs of AC (Adriamycin (Doxorubicin) and Cyclophosphamide) combination therapy with special reference to GIT (Gastrointestinal system). It was a prospective, descriptive, observational study which included 90 female patients receiving AC combination therapy for their treatment through a purposive sampling and from 01-01-2016 to 31-12-2016 at Cancer Hospital Jamshoro Pakistan. Patients were interviewed during follow up session with a consultant, their response was recorded in a questionnaire and verified through British National formulary (BNF), Hartwig & Siegel scale (ADR severity assessment scale). The survey results shows that AC anticancer combination therapy can result in various disturbances in gastrointestinal tract among which nausea, vomiting decreased appetite and hyperacidity were most common. These must be taken into account and managed with supportive treatme...