Sanjay Mondal - Academia.edu (original) (raw)

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Papers by Sanjay Mondal

Research paper thumbnail of Development of Assay Systems for Amber Codon Decoding at the Steps of Initiation and Elongation in Mycobacteria

Journal of Bacteriology

Genetic analysis of the mechanism of protein synthesis in Gram-positive bacteria has remained lar... more Genetic analysis of the mechanism of protein synthesis in Gram-positive bacteria has remained largely unexplored because of the unavailability of appropriate in vivo assay systems. We developed chloramphenicol acetyltransferase (CAT)-based in vivo reporter systems to study translation initiation and elongation in Mycobacterium smegmatis. The CAT reporters utilize specific decoding of amber codons by mutant initiator tRNA (i-tRNA, metU) molecules containing a CUA anticodon (metUCUA). The assay systems allow structure-function analyses of tRNAs without interfering with the cellular protein synthesis and function with or without the expression of heterologous GlnRS from Escherichia coli. We show that despite their naturally occurring slow-growth phenotypes, the step of i-tRNA formylation is vital in translation initiation in mycobacteria and that formylation-deficient i-tRNA mutants (metUCUA/A1, metUCUA/G72, and metUCUA/G72G73) with a Watson-Crick base pair at the 1·72 position partici...

Research paper thumbnail of Antimicrobial activity of fusidic acid in Escherichia coli is dependent on the relative levels of ribosome recycling factor and elongation factor G

FEMS Microbiology Letters

The antimicrobial activity of the antibiotic fusidic acid in Escherichia coli increases with an i... more The antimicrobial activity of the antibiotic fusidic acid in Escherichia coli increases with an increase in the cellular levels of elongation factor G with respect to ribosome recycling factor.

Research paper thumbnail of Development of Assay Systems for Amber Codon Decoding at the Steps of Initiation and Elongation in Mycobacteria

Journal of Bacteriology

Genetic analysis of the mechanism of protein synthesis in Gram-positive bacteria has remained lar... more Genetic analysis of the mechanism of protein synthesis in Gram-positive bacteria has remained largely unexplored because of the unavailability of appropriate in vivo assay systems. We developed chloramphenicol acetyltransferase (CAT)-based in vivo reporter systems to study translation initiation and elongation in Mycobacterium smegmatis. The CAT reporters utilize specific decoding of amber codons by mutant initiator tRNA (i-tRNA, metU) molecules containing a CUA anticodon (metUCUA). The assay systems allow structure-function analyses of tRNAs without interfering with the cellular protein synthesis and function with or without the expression of heterologous GlnRS from Escherichia coli. We show that despite their naturally occurring slow-growth phenotypes, the step of i-tRNA formylation is vital in translation initiation in mycobacteria and that formylation-deficient i-tRNA mutants (metUCUA/A1, metUCUA/G72, and metUCUA/G72G73) with a Watson-Crick base pair at the 1·72 position partici...

Research paper thumbnail of Antimicrobial activity of fusidic acid in Escherichia coli is dependent on the relative levels of ribosome recycling factor and elongation factor G

FEMS Microbiology Letters

The antimicrobial activity of the antibiotic fusidic acid in Escherichia coli increases with an i... more The antimicrobial activity of the antibiotic fusidic acid in Escherichia coli increases with an increase in the cellular levels of elongation factor G with respect to ribosome recycling factor.

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