Alberdan Santos - Academia.edu (original) (raw)

Papers by Alberdan Santos

Research paper thumbnail of Green Extraction by Ultrasound, Microencapsulation by Spray Drying and Antioxidant Activity of the Tucuma Coproduct (Astrocaryum vulgare Mart.) Almonds

Biomolecules, Apr 8, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Crude Enzyme Concentrate of Filamentous Fungus Hydrolyzed Chitosan to Obtain Oligomers of Different Sizes

Polymers

Chitosan is a non-cytotoxic polysaccharide that, upon hydrolysis, releases oligomers of different... more Chitosan is a non-cytotoxic polysaccharide that, upon hydrolysis, releases oligomers of different sizes that may have antioxidant, antimicrobial activity and the inhibition of cancer cell growth, among other applications. It is, therefore, a hydrolysis process with great biotechnological relevance. Thus, this study aims to use a crude enzyme concentrate (CEC) produced by a filamentous fungus to obtain oligomers with different molecular weights. The microorganism was cultivated in a liquid medium (modified Czapeck—with carboxymethylcellulose as enzyme inducer). The enzymes present in the CEC were identified by LC-MS/MS, with an emphasis on cellobiohydrolase (E.C 3.2.1.91). The fungus of the Aspergillus genus was identified by amplifying the ITS1-5.8S-ITS2 rDNA region and metaproteomic analysis, where the excreted enzymes were identified with sequence coverage greater than 84% to A. nidulans. Chitosan hydrolysis assays compared the CEC with the commercial enzyme (Celluclast 1.5 L®). T...

Research paper thumbnail of Ethanol Associated with Ultrasound Efficiently Extracted Carotenoids from Peels of Peach Palm Fruits (Bactris Gasipaes Kunth) Without Altering Carotenoid Profile

Social Science Research Network, 2022

Research paper thumbnail of Andiroba oil and nanoemulsion (Carapa guianensis Aublet) reduce lesion severity caused by the antineoplastic agent doxorubicin in mice

Biomedicine & Pharmacotherapy

Doxorubicin (DOX) is an anthracycline antibiotic used in the fight against many types of cancer. ... more Doxorubicin (DOX) is an anthracycline antibiotic used in the fight against many types of cancer. Although it is quite effective for this purpose, its clinical use is limited by its severe side effects, highlighting the relevance of efforts to identify substances that act to minimize these effects. In this work, we sought to verify the ability of andiroba oil (AO) and a nanoemulsion of andiroba oil (AN) to lessen the side effects of DOX. The animals were separated into 7 groups with 6 animals each: mice treated with AO (2000 mg/kg), AN (2000 mg/kg), the antineoplastic agent DOX (40 mg/kg), AO+DOX, AN+DOX and solvent controls was used of negative control (corn oil and nanoemulsion surfactant). AO and AN were administered for 14 consecutive days orally by gavage and on the 13th day, applied DOX by intraperitoneal route (i.p.), in order to evaluate the protective potential of andiroba. The animals were euthanized on the 15th day. Hematological, biochemical, histological, and immunohistochemical parameters were analyzed. Andiroba reduced several aspects of the severity of lesions caused by DOX, decreasing hematotoxicity and the severity of histological changes in the liver and kidneys, and reducing the frequency of apoptotic cell death. In many cases, AN showed greater efficacy than AO alone, reflecting the feasibility of using this nanotechnology to improve the pharmacokinetics of lipid compounds in the body. The study sheds new light on the therapeutic benefits of andiroba and suggests new ways for investigating how the quantity and quality of lipid compounds affect exposed organisms.

Research paper thumbnail of Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L

Arabian Journal of Chemistry

Research paper thumbnail of POSTER PRESENTATION Open Access Andiroba oil: a strategic approach to detect antioxidant activity in different lipid groups by TLC

The search for natural sources of molecules that exhibit biological activity has intensified in t... more The search for natural sources of molecules that exhibit biological activity has intensified in the last 20 years. The andiroba (Carapa guianensis) oil is known for its medicinal actions [1], and biological activities, among them the anti-oxidant action [2]. The use of traditional techniques for the determination of antioxidant activity in vegetable oils faces strong competition from modern techniques and more updated, but the thin layer chromatography (TLC) is presented as effective, quick, simple and low cost. In this study we applied the technique chromatographic separa-tion of lipid groups in the crud andiroba oil [3] in order to identify groups that have antioxidant activity [4]. The crud andiroba oil used was purchased at a market in Belém / Pa. The oil was applied on TLC plate (10x20cm) eluted with a solvent system consisting of hexane: diethyl ether:

Research paper thumbnail of Coletânea de Atividades em Pesquisa Científica e Inovação Tecnológica

Research paper thumbnail of Systematic investigation and lipidomic profiles composition characterization in leaves of five Amaryllidaceae species by HRGC-MS technique

South African Journal of Botany, 2021

Abstract This study describes a comparative of the lipidomic profiles of the leaves of the specie... more Abstract This study describes a comparative of the lipidomic profiles of the leaves of the species Crinum americanum L, Crinum jagus, Crinum erubescens, Hippeastrum puniceum and Hymenocallis littoralis belonging to the Amaryllidaceae family and the potential pharmaceutical metabolites applications. The extract obtained by ultrasound-assisted extraction with n-hexane was submitted to transesterification with MeOH/KOH and derivatization with BSTFA+1% of CSTS. It was applied analytical thin-layer chromatography with chemical reaction (ATLC-CR) and gas chromatography coupled to mass spectroscopy (GC/MS). To discriminate the similarities and differences between the researched species, a critical statistical analysis of the results was performed applying the Heatmap and principal component analysis (PCA) methodologies. The results indicated the presence of the following metabolic classes: monoacylglycerol (MAG), diacylglycerol (DAG), triacylglycerol (TAG), free fatty acids (AGL), steroids (ST), terpenes (TP), phospholipids (FL) and tocopherols (TF), and identify 50 metabolites in the samples, mainly saturated and unsaturated fatty acids and organic acids. Hexadecanoic acid is the major metabolite of all the investigated species, ranging from 16.00% to 23.44% of the extract's composition. The results of statistical analysis also reveal that the extracts from five Amaryllidaceae species have diverse metabolites with demonstrated the grouping between the same genera due to their lipid composition.

Research paper thumbnail of Genomic screening and molecular dynamics simulations of cyanovirin‐N homologs from cyanobacteria phylum

Proteins: Structure, Function, and Bioinformatics, 2020

The phylum cyanobacteria are one of the most ancient groups of organisms on the planet and are we... more The phylum cyanobacteria are one of the most ancient groups of organisms on the planet and are well recognized due to its wide distribution, ecological role, and biotechnological potential. Cyanobacterial lectins are being extensively explored due to their antiviral activity, mainly because of their capacity of inhibiting HIV strains from infecting human cells by gp120 and gp41 binding. Cianovirin‐N from Nostoc ellipsosporum was the first lectin isolated with this property. Since then, various homologs have been discovered and characterized. In this article, we present results of a genomic screening to find cyanovirin‐N homologs (CVNH) in all cyanobacteria genomes available in the GenBank, resulting in 155 CVNH proteins with 63 presenting significant identity differences of cyanovirin‐N. Homology modeling and molecular dynamics were employed to characterize 18 unexplored models and their functional capacity of binding to Manα(1‐2)Man. Results presented here support the hypothesis of...

Research paper thumbnail of Thermal-Stability of Enzyme Activity and its Application in The Hydrolysis of Starchy Residue From Mandioca Processing

International journal of scientific research, 2015

Natural occurring fungi have been investigated as producers of amylase degrading starchy residue ... more Natural occurring fungi have been investigated as producers of amylase degrading starchy residue from mandioca (Cassava) flour processing. A high degrading starch fungi strain was isolated and identified as Trichodermareesei CFIBA001 , and was capable to hydrolyze starchy residue completely (20 g l -1 ) in three days of incubation at 30 o C and 160rpm. The amylase present in the crude extract showed high activity with thermal-stability over 3h at 60 o C with pH 5.5. This is an important result for a fungus strain with the potentiality to use in the exploitation of the mandioca´s residue discarded from mandioca flour manufactory to produce sugars that could be used in the bioetanol producing process.

Research paper thumbnail of Author response for "Genomic screening and molecular dynamics simulations of cyanovirin‐N homologs from cyanobacteria phylum

Research paper thumbnail of Cultivo in Vitro De Jambu Com Diferentes Reguladores De Crescimento

Horticultura Brasileira, 2020

Acmella oleracea , conhecida como jambu, e uma planta medicinal pertencente a familia Asteraceae.... more Acmella oleracea , conhecida como jambu, e uma planta medicinal pertencente a familia Asteraceae. Devido as varias propriedades medicinais e cosmeticas tem aumentado o interesse e a procura no setor farmaceutico para uma propagacao em larga escala. O presente trabalho objetivou investigar o desenvolvimento in vitro do jambu com diferentes reguladores de crescimento. Inicialmente, apos assepsia as sementes foram inoculadas em meios diferentes (MS, ½MS e agua-agar) para germinacao in vitro . Para o estabelecimento da micropropagacao foram utilizados segmentos nodais das plântulas germinadas in vitro em meio MS com BAP a 0,0 (T1);0,125(T2);0,25(T3); 0,50(T4) e 0,75 mg.L -1 (T5).Para a inducao de calos foram utilizados segmentos nodais em meio MS com 2,4-D a 0,0(T1); 0,25(T2); 0,50(T3); 0,75(T4) e 1,0(T5) mg.L -1 . Todos os meios foram suplementados com sacarose a 30 g.L -1 e agar a 8 g.L -1 . As culturas foram mantidas a 25 ± 2 o C e fotoperiodo de 16h. As maiores taxas de germinacao f...

Research paper thumbnail of Production of Lipolytic Enzymes by Endophytic Fungi and Its Use in the Hydrolysis of Crude Oil

International journal of scientific research, 2015

Endophytic fungi were isolated from the seeds of oil palm ( Elaeisguineensis Jacq) by biomonitori... more Endophytic fungi were isolated from the seeds of oil palm ( Elaeisguineensis Jacq) by biomonitoring lipase activity on semi-solid media with chromogenic substrate. Molecular and classical methods were used to identify the species, and the selected fungus was confirmed as Penicilliumcitrinum . Method for thermal stability of the enzyme carried on crude oil hydrolysis. The fermentation was carried out in shake flasks and results were compared with control at 30°C and pH 6.8 Induction of lipase activity promoted a concentrated fraction of extra-cell lipase (FOL) with 137mg/L and activity of 12000 U/L in submerged culture. The hydrolysis yielded Y P/So = 0.4623 of free fatty acids. It was not observed that crude oil inhibit lipase activity, only products as known, whereas refined oil enhanced lipase activity. The micro-organism showed a production of a robust enzyme with prospects for technological application.

Research paper thumbnail of The effect of drying of Piper hispidinervium by different methods and its influence on the yield of essential oil and safrole

Information Processing in Agriculture, 2021

Research paper thumbnail of A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

Arabian Journal of Chemistry, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Metabolomics techniques applied in the investigation of phenolic acids from the agro-industrial by-product of Carapa guianensis Aubl

Arabian Journal of Chemistry, 2021

Research paper thumbnail of Bioculturalidade, conservação e biotecnologia na Amazônia Oriental

Research paper thumbnail of Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose-rich liquor as substrates

Brazilian Journal of Chemical Engineering, 2020

Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due ... more Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due to the high concentration of residual sugars. Nevertheless, several potentially toxic byproducts are also present. However, a few microorganisms are able to overcome this toxicity by growing on these liquors. Twenty-five filamentous fungi were evaluated in submerged cultivation, but none was able to grow using liquor at a concentration of 100% as the liquid medium. However, six fungi were selected for enzyme induction after being grown in diluted liquor at 50% (v/v) using two feed pulses. Induction experiments were performed using 1% untreated and pretreated sugarcane bagasse. FPase and xylanase activities were detected for all six fungi in submerged cultivation, whereas β-glucosidase was observed in four fungi. The highest xylanase activity (28.8 IU mL −1) was at 72 h for T. harzianum P49P11 using pretreated-SCB as an inducer. This work showed a successful alternative for the final destination of liquor residue as substrate for fungi cultivation prior to enzyme production.

Research paper thumbnail of Andiroba oil (Carapa guianensisAubl) shows cytotoxicity but no mutagenicity in the ACPP02 gastric cancer cell line

Journal of Applied Toxicology, 2020

Andiroba (Carapa guianensisAubl) is an Amazonian plant whose oil has been widely used in traditio... more Andiroba (Carapa guianensisAubl) is an Amazonian plant whose oil has been widely used in traditional medicine for various purposes, including anti‐inflammation. Research reports indicate that the oil can confer antitumor activity due to the presence of fatty acids, which can directly influence cell death mechanisms. Thus, andiroba oil (AO) has gained interest for its potential to be used in antineoplastic therapies. Here, we report an in vitro analysis of the cytotoxic and mutagenic potential of AO in the gastric cancer cell line, ACP02. Cell survival was assessed by the MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay, differential staining with ethidium bromide and acridine orange assessed apoptosis‐necrosis, and mutagenesis was assessed by the micronucleus test. The apolar oil was first diluted in 0.1% dimethyl sulfoxide (DMSO) and then further diluted to six concentrations (0.01, 0.1, 1, 10 and 100 μg/mL and 1 mg/mL) in RPMI medium. Controls included RPMI...

Research paper thumbnail of Aqueous ethanol extract of Libidibia ferrea (Mart. Ex Tul) L.P. Queiroz (juca) exhibits antioxidant and migration-inhibiting activity in human gastric adenocarcinoma (ACP02) cells

PLOS ONE, 2020

Libidibia ferrea (juca) is a plant belonging to the Fabaceae (Leguminosae) family, whose antioxid... more Libidibia ferrea (juca) is a plant belonging to the Fabaceae (Leguminosae) family, whose antioxidant activity has been widely described in the literature. We evaluated this parameter of Aqueous ethanol extract (AE), ethyl acetate (ACO), chloroform (CLO) and hexane (HEX) extracts of L. ferrea. We then tested the most active extract for its toxicity and ability to inhibit migratory activity in the ACP02 gastric adenocarcinoma cell line in vitro. The AE and ACO extracts both had antioxidant activity, the AE extract showing greater potential. This may reflect that both extracts contained phenolic compounds. Although AE extract showed no cytotoxic, mutagenic or genotoxic effect, it altered cell morphology and migration activity. Analysis of apoptosis/necrosis indicated that this parameter does not appear to account for the apparent ability of AE to inhibit cancer cell migration. We speculate that the morphological changes in AE-treated cells could be due to cytoskeleton alterations related to the presence of myo-inositol in AE extract. Together, our results demonstrate this extract of L. ferrea can act as an exogenous antioxidant and might prove useful in efforts to fight secondary tumors.

Research paper thumbnail of Green Extraction by Ultrasound, Microencapsulation by Spray Drying and Antioxidant Activity of the Tucuma Coproduct (Astrocaryum vulgare Mart.) Almonds

Biomolecules, Apr 8, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Crude Enzyme Concentrate of Filamentous Fungus Hydrolyzed Chitosan to Obtain Oligomers of Different Sizes

Polymers

Chitosan is a non-cytotoxic polysaccharide that, upon hydrolysis, releases oligomers of different... more Chitosan is a non-cytotoxic polysaccharide that, upon hydrolysis, releases oligomers of different sizes that may have antioxidant, antimicrobial activity and the inhibition of cancer cell growth, among other applications. It is, therefore, a hydrolysis process with great biotechnological relevance. Thus, this study aims to use a crude enzyme concentrate (CEC) produced by a filamentous fungus to obtain oligomers with different molecular weights. The microorganism was cultivated in a liquid medium (modified Czapeck—with carboxymethylcellulose as enzyme inducer). The enzymes present in the CEC were identified by LC-MS/MS, with an emphasis on cellobiohydrolase (E.C 3.2.1.91). The fungus of the Aspergillus genus was identified by amplifying the ITS1-5.8S-ITS2 rDNA region and metaproteomic analysis, where the excreted enzymes were identified with sequence coverage greater than 84% to A. nidulans. Chitosan hydrolysis assays compared the CEC with the commercial enzyme (Celluclast 1.5 L®). T...

Research paper thumbnail of Ethanol Associated with Ultrasound Efficiently Extracted Carotenoids from Peels of Peach Palm Fruits (Bactris Gasipaes Kunth) Without Altering Carotenoid Profile

Social Science Research Network, 2022

Research paper thumbnail of Andiroba oil and nanoemulsion (Carapa guianensis Aublet) reduce lesion severity caused by the antineoplastic agent doxorubicin in mice

Biomedicine & Pharmacotherapy

Doxorubicin (DOX) is an anthracycline antibiotic used in the fight against many types of cancer. ... more Doxorubicin (DOX) is an anthracycline antibiotic used in the fight against many types of cancer. Although it is quite effective for this purpose, its clinical use is limited by its severe side effects, highlighting the relevance of efforts to identify substances that act to minimize these effects. In this work, we sought to verify the ability of andiroba oil (AO) and a nanoemulsion of andiroba oil (AN) to lessen the side effects of DOX. The animals were separated into 7 groups with 6 animals each: mice treated with AO (2000 mg/kg), AN (2000 mg/kg), the antineoplastic agent DOX (40 mg/kg), AO+DOX, AN+DOX and solvent controls was used of negative control (corn oil and nanoemulsion surfactant). AO and AN were administered for 14 consecutive days orally by gavage and on the 13th day, applied DOX by intraperitoneal route (i.p.), in order to evaluate the protective potential of andiroba. The animals were euthanized on the 15th day. Hematological, biochemical, histological, and immunohistochemical parameters were analyzed. Andiroba reduced several aspects of the severity of lesions caused by DOX, decreasing hematotoxicity and the severity of histological changes in the liver and kidneys, and reducing the frequency of apoptotic cell death. In many cases, AN showed greater efficacy than AO alone, reflecting the feasibility of using this nanotechnology to improve the pharmacokinetics of lipid compounds in the body. The study sheds new light on the therapeutic benefits of andiroba and suggests new ways for investigating how the quantity and quality of lipid compounds affect exposed organisms.

Research paper thumbnail of Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L

Arabian Journal of Chemistry

Research paper thumbnail of POSTER PRESENTATION Open Access Andiroba oil: a strategic approach to detect antioxidant activity in different lipid groups by TLC

The search for natural sources of molecules that exhibit biological activity has intensified in t... more The search for natural sources of molecules that exhibit biological activity has intensified in the last 20 years. The andiroba (Carapa guianensis) oil is known for its medicinal actions [1], and biological activities, among them the anti-oxidant action [2]. The use of traditional techniques for the determination of antioxidant activity in vegetable oils faces strong competition from modern techniques and more updated, but the thin layer chromatography (TLC) is presented as effective, quick, simple and low cost. In this study we applied the technique chromatographic separa-tion of lipid groups in the crud andiroba oil [3] in order to identify groups that have antioxidant activity [4]. The crud andiroba oil used was purchased at a market in Belém / Pa. The oil was applied on TLC plate (10x20cm) eluted with a solvent system consisting of hexane: diethyl ether:

Research paper thumbnail of Coletânea de Atividades em Pesquisa Científica e Inovação Tecnológica

Research paper thumbnail of Systematic investigation and lipidomic profiles composition characterization in leaves of five Amaryllidaceae species by HRGC-MS technique

South African Journal of Botany, 2021

Abstract This study describes a comparative of the lipidomic profiles of the leaves of the specie... more Abstract This study describes a comparative of the lipidomic profiles of the leaves of the species Crinum americanum L, Crinum jagus, Crinum erubescens, Hippeastrum puniceum and Hymenocallis littoralis belonging to the Amaryllidaceae family and the potential pharmaceutical metabolites applications. The extract obtained by ultrasound-assisted extraction with n-hexane was submitted to transesterification with MeOH/KOH and derivatization with BSTFA+1% of CSTS. It was applied analytical thin-layer chromatography with chemical reaction (ATLC-CR) and gas chromatography coupled to mass spectroscopy (GC/MS). To discriminate the similarities and differences between the researched species, a critical statistical analysis of the results was performed applying the Heatmap and principal component analysis (PCA) methodologies. The results indicated the presence of the following metabolic classes: monoacylglycerol (MAG), diacylglycerol (DAG), triacylglycerol (TAG), free fatty acids (AGL), steroids (ST), terpenes (TP), phospholipids (FL) and tocopherols (TF), and identify 50 metabolites in the samples, mainly saturated and unsaturated fatty acids and organic acids. Hexadecanoic acid is the major metabolite of all the investigated species, ranging from 16.00% to 23.44% of the extract's composition. The results of statistical analysis also reveal that the extracts from five Amaryllidaceae species have diverse metabolites with demonstrated the grouping between the same genera due to their lipid composition.

Research paper thumbnail of Genomic screening and molecular dynamics simulations of cyanovirin‐N homologs from cyanobacteria phylum

Proteins: Structure, Function, and Bioinformatics, 2020

The phylum cyanobacteria are one of the most ancient groups of organisms on the planet and are we... more The phylum cyanobacteria are one of the most ancient groups of organisms on the planet and are well recognized due to its wide distribution, ecological role, and biotechnological potential. Cyanobacterial lectins are being extensively explored due to their antiviral activity, mainly because of their capacity of inhibiting HIV strains from infecting human cells by gp120 and gp41 binding. Cianovirin‐N from Nostoc ellipsosporum was the first lectin isolated with this property. Since then, various homologs have been discovered and characterized. In this article, we present results of a genomic screening to find cyanovirin‐N homologs (CVNH) in all cyanobacteria genomes available in the GenBank, resulting in 155 CVNH proteins with 63 presenting significant identity differences of cyanovirin‐N. Homology modeling and molecular dynamics were employed to characterize 18 unexplored models and their functional capacity of binding to Manα(1‐2)Man. Results presented here support the hypothesis of...

Research paper thumbnail of Thermal-Stability of Enzyme Activity and its Application in The Hydrolysis of Starchy Residue From Mandioca Processing

International journal of scientific research, 2015

Natural occurring fungi have been investigated as producers of amylase degrading starchy residue ... more Natural occurring fungi have been investigated as producers of amylase degrading starchy residue from mandioca (Cassava) flour processing. A high degrading starch fungi strain was isolated and identified as Trichodermareesei CFIBA001 , and was capable to hydrolyze starchy residue completely (20 g l -1 ) in three days of incubation at 30 o C and 160rpm. The amylase present in the crude extract showed high activity with thermal-stability over 3h at 60 o C with pH 5.5. This is an important result for a fungus strain with the potentiality to use in the exploitation of the mandioca´s residue discarded from mandioca flour manufactory to produce sugars that could be used in the bioetanol producing process.

Research paper thumbnail of Author response for "Genomic screening and molecular dynamics simulations of cyanovirin‐N homologs from cyanobacteria phylum

Research paper thumbnail of Cultivo in Vitro De Jambu Com Diferentes Reguladores De Crescimento

Horticultura Brasileira, 2020

Acmella oleracea , conhecida como jambu, e uma planta medicinal pertencente a familia Asteraceae.... more Acmella oleracea , conhecida como jambu, e uma planta medicinal pertencente a familia Asteraceae. Devido as varias propriedades medicinais e cosmeticas tem aumentado o interesse e a procura no setor farmaceutico para uma propagacao em larga escala. O presente trabalho objetivou investigar o desenvolvimento in vitro do jambu com diferentes reguladores de crescimento. Inicialmente, apos assepsia as sementes foram inoculadas em meios diferentes (MS, ½MS e agua-agar) para germinacao in vitro . Para o estabelecimento da micropropagacao foram utilizados segmentos nodais das plântulas germinadas in vitro em meio MS com BAP a 0,0 (T1);0,125(T2);0,25(T3); 0,50(T4) e 0,75 mg.L -1 (T5).Para a inducao de calos foram utilizados segmentos nodais em meio MS com 2,4-D a 0,0(T1); 0,25(T2); 0,50(T3); 0,75(T4) e 1,0(T5) mg.L -1 . Todos os meios foram suplementados com sacarose a 30 g.L -1 e agar a 8 g.L -1 . As culturas foram mantidas a 25 ± 2 o C e fotoperiodo de 16h. As maiores taxas de germinacao f...

Research paper thumbnail of Production of Lipolytic Enzymes by Endophytic Fungi and Its Use in the Hydrolysis of Crude Oil

International journal of scientific research, 2015

Endophytic fungi were isolated from the seeds of oil palm ( Elaeisguineensis Jacq) by biomonitori... more Endophytic fungi were isolated from the seeds of oil palm ( Elaeisguineensis Jacq) by biomonitoring lipase activity on semi-solid media with chromogenic substrate. Molecular and classical methods were used to identify the species, and the selected fungus was confirmed as Penicilliumcitrinum . Method for thermal stability of the enzyme carried on crude oil hydrolysis. The fermentation was carried out in shake flasks and results were compared with control at 30°C and pH 6.8 Induction of lipase activity promoted a concentrated fraction of extra-cell lipase (FOL) with 137mg/L and activity of 12000 U/L in submerged culture. The hydrolysis yielded Y P/So = 0.4623 of free fatty acids. It was not observed that crude oil inhibit lipase activity, only products as known, whereas refined oil enhanced lipase activity. The micro-organism showed a production of a robust enzyme with prospects for technological application.

Research paper thumbnail of The effect of drying of Piper hispidinervium by different methods and its influence on the yield of essential oil and safrole

Information Processing in Agriculture, 2021

Research paper thumbnail of A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

Arabian Journal of Chemistry, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Metabolomics techniques applied in the investigation of phenolic acids from the agro-industrial by-product of Carapa guianensis Aubl

Arabian Journal of Chemistry, 2021

Research paper thumbnail of Bioculturalidade, conservação e biotecnologia na Amazônia Oriental

Research paper thumbnail of Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose-rich liquor as substrates

Brazilian Journal of Chemical Engineering, 2020

Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due ... more Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due to the high concentration of residual sugars. Nevertheless, several potentially toxic byproducts are also present. However, a few microorganisms are able to overcome this toxicity by growing on these liquors. Twenty-five filamentous fungi were evaluated in submerged cultivation, but none was able to grow using liquor at a concentration of 100% as the liquid medium. However, six fungi were selected for enzyme induction after being grown in diluted liquor at 50% (v/v) using two feed pulses. Induction experiments were performed using 1% untreated and pretreated sugarcane bagasse. FPase and xylanase activities were detected for all six fungi in submerged cultivation, whereas β-glucosidase was observed in four fungi. The highest xylanase activity (28.8 IU mL −1) was at 72 h for T. harzianum P49P11 using pretreated-SCB as an inducer. This work showed a successful alternative for the final destination of liquor residue as substrate for fungi cultivation prior to enzyme production.

Research paper thumbnail of Andiroba oil (Carapa guianensisAubl) shows cytotoxicity but no mutagenicity in the ACPP02 gastric cancer cell line

Journal of Applied Toxicology, 2020

Andiroba (Carapa guianensisAubl) is an Amazonian plant whose oil has been widely used in traditio... more Andiroba (Carapa guianensisAubl) is an Amazonian plant whose oil has been widely used in traditional medicine for various purposes, including anti‐inflammation. Research reports indicate that the oil can confer antitumor activity due to the presence of fatty acids, which can directly influence cell death mechanisms. Thus, andiroba oil (AO) has gained interest for its potential to be used in antineoplastic therapies. Here, we report an in vitro analysis of the cytotoxic and mutagenic potential of AO in the gastric cancer cell line, ACP02. Cell survival was assessed by the MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay, differential staining with ethidium bromide and acridine orange assessed apoptosis‐necrosis, and mutagenesis was assessed by the micronucleus test. The apolar oil was first diluted in 0.1% dimethyl sulfoxide (DMSO) and then further diluted to six concentrations (0.01, 0.1, 1, 10 and 100 μg/mL and 1 mg/mL) in RPMI medium. Controls included RPMI...

Research paper thumbnail of Aqueous ethanol extract of Libidibia ferrea (Mart. Ex Tul) L.P. Queiroz (juca) exhibits antioxidant and migration-inhibiting activity in human gastric adenocarcinoma (ACP02) cells

PLOS ONE, 2020

Libidibia ferrea (juca) is a plant belonging to the Fabaceae (Leguminosae) family, whose antioxid... more Libidibia ferrea (juca) is a plant belonging to the Fabaceae (Leguminosae) family, whose antioxidant activity has been widely described in the literature. We evaluated this parameter of Aqueous ethanol extract (AE), ethyl acetate (ACO), chloroform (CLO) and hexane (HEX) extracts of L. ferrea. We then tested the most active extract for its toxicity and ability to inhibit migratory activity in the ACP02 gastric adenocarcinoma cell line in vitro. The AE and ACO extracts both had antioxidant activity, the AE extract showing greater potential. This may reflect that both extracts contained phenolic compounds. Although AE extract showed no cytotoxic, mutagenic or genotoxic effect, it altered cell morphology and migration activity. Analysis of apoptosis/necrosis indicated that this parameter does not appear to account for the apparent ability of AE to inhibit cancer cell migration. We speculate that the morphological changes in AE-treated cells could be due to cytoskeleton alterations related to the presence of myo-inositol in AE extract. Together, our results demonstrate this extract of L. ferrea can act as an exogenous antioxidant and might prove useful in efforts to fight secondary tumors.