Sara larki - Academia.edu (original) (raw)

Papers by Sara larki

Antimicrobial Agents and Chemotherapy, Oct 1, 2014

Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determi... more Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the essential oil (EO) from aromatic water (AW) of Zataria multiflora. Thymol (66.9%), carvacrol (15.2%), and carvone (7.3%) were found to be the major EO constituents. Eighty laboratory BALB/c mice were infected intraperitoneally by injection of 1,500 viable protoscolices and were divided into prevention (40 mice) and therapeutic (40 mice) groups. To prove the preventive effect of the Z. multiflora AW on development of hydatid cysts, the 40 infected mice were allocated into three treatment groups, including the albendazole group (10 mice that received 150 mg/kg body weight/day for 10 days), the Z. multiflora AW group (15 mice that received 20 ml/liter in drinking water for 8 months), and a control group (15 mice that received no treatment). To estimate the therapeutic effect of the Z. multiflora AW on the hydatid cyst, after 8 months of infection, the 15 remaining mice were allocated into three experimental treatment groups of five animals each, including the albendazole group (300 mg/ kg/day for 20 days), Z. multiflora AW group (40 ml/liter in drinking water for 30 days), and control group (no treatment). All mice were then euthanized, and the sizes and weights of the cysts as well as their ultrastructural changes were investigated. The weights and sizes of the hydatid cysts significantly decreased upon treatment with the Z. multiflora AW in both the preventive and therapeutic groups (P < 0.05). The results of scanning electron microscopy also showed considerable damage in the germinal layer of the hydatid cysts recovered from the treated animals.

Successful development of free-living stages of parasitic helminths depends on larva ability to s... more Successful development of free-living stages of parasitic helminths depends on larva ability to survive, develop, and hatch. In this study, we aimed to study the host role in the hatching process of Fasciola species. Methods: Fasciola hepatica and Fasciola gigantica eggs were collected from adult worms that originated from naturally infected sheep and cattle livers and were incubated at 26±1°C for 15 days. The percentage of hatched and developed eggs were obtained for each isolate under a light microscope. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) was applied to identify the F. hepatica and F. gigantica species. Results: Our findings showed no significant differences in the development rates of F. gigantica and F. hepatica eggs in sheep (69.32% and 72.71%) and cattle (73.56% and74.69%). However, the rates of hatched eggs of F. gigantica and F. hepatica originated from cattle (69.19% and 62.36%) were almost twice the rates in sheep (31.69% and 32.59%), indicating a significant difference. Conclusion: This study demonstrated that host species significantly affect the hatching of Fasciola eggs as the hatching rates of F. gigantica and F. hepatica originated from cattle were higher than those taken from sheep did not affect their larval development. Thus, in addition to environmental factors, the hatching phenomenon is influenced by host species.

Kocatepe Veterinary Journal, 2020

Monogeneans gill parasites of the diversified Dactylogyrus genus are host-specific to freshwater ... more Monogeneans gill parasites of the diversified Dactylogyrus genus are host-specific to freshwater fish of the Cyprinidae family. Accurate detection of various Dactylogyrus species is very time-consuming and requires highly experienced personnel; to overcome this problem, the genomic DNA technology has been recently used to detect Dactylogyrus spp. In this study, 23 Cyprinus carpio and 19 Ctenopharyngodon idella isolates were collected from different farms and local markets of Ahvaz, southwest of Iran. The gill arches of each fish were carefully examined under a light microscope. Dactylogyrus species was identified morphologically. Identical species were kept in ethanol for molecular study. Molecular analysis was performed using amplification of the ITS-1 region of the ribosomal RNA gene of the parasite. Then, the amplified PCR products were sequenced. The aligned nucleotide sequences were analyzed to construct the phylogenetic tree of the identified species. The results revealed two lineages including D. extensus isolated from common carp and D. lamellatus taken from grass carp fish. Phylogenetic analysis showed that the detected D. extensus and D. lamellatus isolates from Khuzestan region were clustered with some Iranian (Guilan), Chinese and Czech isolates. Importing fingerlings from Guilan Province could lead to introducing monogenean infections to native cyprinid fish.

Research Square (Research Square), Mar 10, 2022

An ancylostomatid Kalicephalus spp. is the common parasitic intestinal nematode of reptiles. West... more An ancylostomatid Kalicephalus spp. is the common parasitic intestinal nematode of reptiles. West-Asian blunt-nosed viper, a venomous snake found in extensive areas of Iran. Between June-September 2017, two dead viper snakes were referred to the parasitology laboratory and examined for intestinal parasites. Several white elongated roundworms were collected and fixed to identify under light and scanning electron microscopes (SEM) based on morphological and molecular characteristics. For the molecular survey, some parts of identified worms were extracted and the ITS of nuclear ribosomal DNA (rDNA) amplified by PCR reaction. All collected hookworms were taxonomically identified as Kalicephalus viperae viperae and exposited in an individual branch far from of Ancylostomatidae species and close to A. braziliense with 88% discrepancies in the phylogenetic tree. The morphological characteristics and a large part of K. viperea viperea rDNA nucleotide sequence was reported in viper snakes for the first time in the world and Iran.

Jentashapir Journal of Cellular and Molecular Biology

Background: The lizardfish is an economically important fish in the Persian Gulf with high rates ... more Background: The lizardfish is an economically important fish in the Persian Gulf with high rates of parasitic infections. Microsporidia species, as opportunistic parasites, cause several disorders, which in turn result in economic problems. Objectives: The main objective was to evaluate Heterosporis sp. infection in Persian Gulf lizardfish using the small subunit ribosomal RNA phylogenetics to describe and classification of the unknown microsporidia species as well as morphological characteristics. Methods: The abdominal cavities of fifty specimens of lizardfish, Saurida undosquamis, were examined using morphological and molecular techniques. Some irregular whitish cyst-like were fixed for histopathological and transmission electron observations. The small subunit ribosomal genomic DNA was studied and a 1,279 bp genomic sequence was amplified and investigated for molecular analysis. Results: Twenty-two (out of fifty) specimens were infected with irregular whitish microsporidian cyst...

Iranian Journal of Parasitology, 2018

Background: Despite ducks being birds resistant to infection, the favorable habitat of ducks such... more Background: Despite ducks being birds resistant to infection, the favorable habitat of ducks such as subtropical climate or stagnant water is also a perfect place for survival of the parasites. Methods: This study was conducted from Dec 2014 to Apr 2015 to determine the prevalence of gastrointestinal parasites of domestic ducks in Ahvaz and environs, southwest of Iran. Overall, 41 fresh fecal samples were collected and prepared using formol-ether concentration, modified Ziehl-Neelsen, sheather`s floatation and zinc sulfate sedimentation methods. Light microscopic morphometry was used for identification of helminth eggs and oocysts. Results: 60.97% of ducks were infected with three different nematodes and/or four protozoan parasites. The identified nematodes were Capillaria sp., (50%) Subulura spp. (16.66%) and Echinuria spp. (33.33%). The protozoan oocystes were Cryptosporidium spp. (50%) and coccidian species (%58.33) and included Wenionella philiplevinei, Tyzerria spp. and Isospor...

Journal of Medical Microbiology and Infectious Diseases, 2020

Introduction: Successful development of free-living stages of parasitic helminths depends on larv... more Introduction: Successful development of free-living stages of parasitic helminths depends on larva ability to survive, develop, and hatch. In this study, we aimed to study the host role in the hatching process of Fasciola species. Methods: Fasciola hepatica and Fasciola gigantica eggs were collected from adult worms that originated from naturally infected sheep and cattle livers and were incubated at 26±1°C for 15 days. The percentage of hatched and developed eggs were obtained for each isolate under a light microscope. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) was applied to identify the F. hepatica and F. gigantica species. Results: Our findings showed no significant differences in the development rates of F. gigantica and F. hepatica eggs in sheep (69.32% and 72.71%) and cattle (73.56% and74.69%). However, the rates of hatched eggs of F. gigantica and F. hepatica originated from cattle (69.19% and 62.36%) were almost twice the rat...

Kocatepe Veterinary Journal

TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES

Zahedan Journal of Research in Medical Sciences

Antimicrobial Agents and Chemotherapy, 2014

ABSTRACTGas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to... more ABSTRACTGas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the essential oil (EO) from aromatic water (AW) ofZataria multiflora. Thymol (66.9%), carvacrol (15.2%), and carvone (7.3%) were found to be the major EO constituents. Eighty laboratory BALB/c mice were infected intraperitoneally by injection of 1,500 viable protoscolices and were divided into prevention (40 mice) and therapeutic (40 mice) groups. To prove the preventive effect of theZ. multifloraAW on development of hydatid cysts, the 40 infected mice were allocated into three treatment groups, including the albendazole group (10 mice that received 150 mg/kg body weight/day for 10 days), theZ. multifloraAW group (15 mice that received 20 ml/liter in drinking water for 8 months), and a control group (15 mice that received no treatment). To estimate the therapeutic effect of theZ. multifloraAW on the hydatid cyst, after 8 months of infection, the 15 ...

Comparative Clinical Pathology, 2014

The phenolic compounds of Zataria multiflora extract, were identified by HPLC analysis. Gallic ac... more The phenolic compounds of Zataria multiflora extract, were identified by HPLC analysis. Gallic acid, catechin, caffeic acid, and quercetin were found to be the major phenolic compounds. Eighty healthy laboratory Balb/C mice were infected intraperitoneally by injection of 1500 viable protoscoleces and were divided into prevention (40 mice) and therapeutic (40 mice) groups. To prove the preventive effect of Z. multiflora extract on development of hydatid cyst, the 40 infected animals were allocated into three treatment groups including Z. multiflora (4 g/l in drinking water for 8 months), albendazole (150 mg/kg BW/day for 10 days) and untreated (control) group. To estimate the therapeutic effect of Z. multiflora extract on the hydatid cyst, after 8 months of infection, the infected mice were allocated into three experimental treatment groups including Z. multiflora (8 g/l in drinking water for 30 days), albendazole (300 mg/kg BW/day for 20 days) and untreated (control) group. At the end of the treatment period, all mice were euthanized and necropsied, the hydatid cysts were carefully removed, weighed and their size were recorded. Weight and size of the hydatid cysts significantly decreased (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05) upon the treatment with Z. multiflora extract in both prevention and therapeutic groups. The germinal layer of the hydatid cysts recovered from the treated mice, either from the prevention or therapeutic group, were completely damaged at ultrastructural level by scanning electron microscopy.

Parasitology Research, 2010

The most confident way for treatment of hydatid cyst is surgical operation. Spillage of the cyst ... more The most confident way for treatment of hydatid cyst is surgical operation. Spillage of the cyst contents during the operation is the major cause of recurrence after hydatid cyst surgery. Instillation of scolicidal agent into hydatid cyst is the most commonly employed measure to prevent this complication. In the present study, the scolicidal effect of highly acidic and alkaline solutions is investigated. Protoscoleces were collected aseptically from sheep livers containing hydatid cyst. Acidic solutions with pH 1, 2, 3, and 4 and alkaline solutions with pH 11, 12, 13, and 14 were used for 5,10, and 15 min in the experiments. Viability of protoscoleces was assessed by 0.1% eosin staining. Scolicidal effect of acidic solution with pH 1 after 5 min and with pH 2 and 3 after 10 min was 100%. Scolicidal effect of acidic solution with pH 2 and 3 after 5 min was 99.6% and 98.7%, respectively. Acidic solution with pH 4 after 5, 10, and 15 min killed 15.5%, 21.5%, and 22.6% of protoscoleces, respectively. Alkaline solution with pH 14 after 5 min and with pH 13 after 15 min killed all protoscoleces. The scolicidal effect of alkaline solution with pH 13 after 5 and 10 min was also 97.5% and 99.7%, respectively. These values for alkaline solution with pH 12 were 29.33%, 33.44% and 37.09%, respectively. The scolicidal effect of solution with pH 11 was 24.5%, 30.5%, and 31.3%, respectively. Although the in vitro scolicidal effect of highly acidic or alkaline solutions was satisfactory in our study, in vivo efficacy of these solutions and also possible side effects, remain to be more investigated.

Parasitology Research, 2011

Four Sarcocystis species, i.e., Sarcocystis fusiformis and Sarcocystis buffalonis with cats as de... more Four Sarcocystis species, i.e., Sarcocystis fusiformis and Sarcocystis buffalonis with cats as definitive hosts, Sarcocystis levinei with dogs as definitive host, and Sarcocystis dubeyi with unknown definitive host, have previously been described from water buffalo (Bubalus bubalis). The aim of the present study was genetic characterization of the causative agent(s) of water buffalo sarcocystosis in Khuzestan Province, western Iran. RFLP-PCR and partial sequence analysis of 18S rDNA gene were used for the genetic characterization of the specimens directly obtained from water buffalo. In RFLP-PCR, four restriction enzymes (Dra1, Ssp1, Fok1 and Bsl1) were used for species discrimination of Sarcocystis spp. in this host. Comparison of the molecular sequencing results and RFLP-PCR pattern of the samples obtained in the present study with those previously reported for different Sarcocystis spp. revealed that all positive Sarcocystis samples represented S. fusiformis. To our knowledge, this is the first demonstration of the existence of S. fusiformis in the Iranian water buffalo population by a genetic approach. In addition, comparison between the alignments between the Iranian 18S rDNA sequences (HQ703791), made in this study, and those previously reported for S. fusiformis in different geographical location (accession nos. AF176927, AF176926, and U03071) showed the occurrence of local genetic polymorphisms and heterogeneity in this ribosomal locus. Despite the occurrence of some genetic variations in the hypervariable regions of the 18S rDNA in S. fusiformis, Dra I restriction site was conserved among all sequences available. According to the present study, it seems that cats have a more significant epidemiological role than dogs in transmission of sarcocystosis agent to water buffalo in Iran.