Mihir Kanti Sarkar - Academia.edu (original) (raw)
Papers by Mihir Kanti Sarkar
Indian Journal of Critical Care Medicine
Background: To evaluate the role of ultrasound during initial fluid resuscitation along with clin... more Background: To evaluate the role of ultrasound during initial fluid resuscitation along with clinical guidance in reducing the incidence of fluid overload on day 3 in children with septic shock. Materials and methods: It was a prospective, parallel limb open-labeled randomized controlled superiority trial done in the PICU of a governmentaided tertiary care hospital in Eastern India. Patient enrolment took place between June 2021 and March 2022. Fifty-six children aged between 1 month and 12 years, with proven or suspected septic shock, were randomized to receive either ultrasound-guided or clinically guided fluid boluses (1:1 ratio) and subsequently followed up for various outcomes. The primary outcome was frequency of fluid overload on day 3 of admission. The treatment group received ultrasound-guided fluid boluses along with the clinical guidance and the control group received the same but without ultrasound guidance upto 60 mL/kg of fluid boluses. Results: The frequency of fluid overload on day 3 of admission was significantly lower in the ultrasound group (25% vs 62%, p = 0.012) as was the median (IQR) cumulative fluid balance percentage on day 3 [6.5 (3.3-10.3) vs 11.3 (5.4-17.5), p = 0.02]. The amount of fluid bolus administered was also significantly lower by ultrasound [median 40 (30-50) vs 50 (40-80) mL/kg, p = 0.003]. Resuscitation time was shorter in the ultrasound group (13.4 ± 5.6 vs 20.5 ± 8 h, p = 0.002). Conclusion: Ultrasound-guided fluid boluses were found to be significantly better than clinically guided therapy, in preventing fluid overload and its associated complications in children with septic shock. These factors make ultrasound a potentially useful tool for resuscitation of children with septic shock in the PICU.
Indian Pediatrics
Objective: To determine the clinical presentation and outcome of children infected with severe ac... more Objective: To determine the clinical presentation and outcome of children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the third wave of the pandemic in India. Method: A review of hospital records was done at a tertiary care hospital, for children seen between 1 and 25 January, 2022. Result: Out of total 112 SARS-CoV-2 positive patients, 17 were hospitalized and 95 were treated in the outpatient department. Among non-hospitalized children, fever was the commonest feature (72, 75.7%), followed by sneezing, and loss of appetite. The median (IQR) duration of symptoms was 2.5 (1.5) days. Among hospitalized children, 10 had co-morbidities and one-third required intensive care unit admission. MIS-C was diagnosed in four patients. Out of 4 mechanically ventilated patients, two had coronavirus disease (COVID) pneumonia. The mean (SD) length of hospital stay was 7.5 (2.5) days. One child with leukemia died during management. Conclusion: During the third wave of the pandemic, most children had symptomatic illnesses, but recovery was fast among non-hospitalized children.
Indian Journal of Pediatrics
Objective To determine the outcomes in children with MIS-C receiving different immunomodulatory t... more Objective To determine the outcomes in children with MIS-C receiving different immunomodulatory treatment. Methods In this multicentric, retrospective cohort study, data regarding treatment and outcomes of children meeting the WHO case definition for MIS-C, were collected. The primary composite outcome was the requirement of vasoactive/inotropic support on day 2 or beyond or need of mechanical ventilation on day 2 or beyond after initiation of immunomodulatory treatment or death during hospitalization in the treatment groups. Logistic regression and propensity score matching analyses were used to compare the outcomes in different treatment arms based on the initial immunomodulation, i.e., IVIG alone, IVIG plus steroids, and steroids alone. Results The data of 368 children (diagnosed between April 2020 and June 2021) meeting the WHO case definition for MIS-C, were analyzed. Of the 368 subjects, 28 received IVIG alone, 82 received steroids alone, 237 received IVIG and steroids, and 21 did not receive any immunomodulation. One hundred fifty-six (42.39%) children had the primary outcome. On logistic regression analysis, the treatment group was not associated with the primary outcome; only the children with shock at diagnosis had higher odds for the occurrence of the outcome [OR (95% CI): 11.4 (5.19-25.0), p < 0.001]. On propensity score matching analysis, the primary outcome was comparable in steroid (n = 45), and IVIG plus steroid (n = 84) groups (p = 0.515). Conclusion While no significant difference was observed in the frequency of occurrence of the primary outcome in different treatment groups, data from adequately powered RCTs are required for definitive recommendations.
IP Journal of Paediatrics and Nursing Science
Parents of the COVID -19 positive hospitalized children are facing different psychological stress... more Parents of the COVID -19 positive hospitalized children are facing different psychological stresses which are affecting child’s care and well-being. To determine the psychological stress, anxiety and depression and to identify the associated influencing factors in mothers of hospitalized COVID-19 infected children In this cross-sectional observational study, 150 mothers of COVID-19 positive hospitalized children in a paediatric tertiary care hospital were randomly selected and studied. Data collection tool was a two part questionnaire; the first part was based on ‘Perceived stress scale-10’ and ‘Hospital anxiety and depression scale’ and the second part included stressors related to COVID-19 pandemic selected based on previous researches. The incidence of these stressors and their significance in causing stress, anxiety and depression was assessed using standard statistical methods.83.4% had moderate psychological stress, 35% had significant anxiety and 38% had significant depressio...
Journal of Tropical Pediatrics, 2020
Background Recently a severe form of COVID-19 infection has been described in a cluster of childr... more Background Recently a severe form of COVID-19 infection has been described in a cluster of children presenting as multisystem inflammatory condition. One of the important spectrum of this condition is incomplete Kawasaki disease (KD). Case report A 5-month-old male child presented with high-spiking fever for 5 days with skin rash, bilateral non-purulent conjunctivitis and irritability. His C-reactive protein was markedly elevated (215.4 mg/l). Echocardiography revealed dilated left main coronary artery (3.0 mm, Z score +4.30) and left anterior descending artery (2.37 mm, Z score +3.76). Concomitantly Reverse Transcription- Polymerase Chain Reaction for COVID-19 was positive on fifth day sample. He was diagnosed as incomplete KD with COVID-19 infection and treated with intravenous immunoglobulin (IVIG) (2 g/kg), oral aspirin and azithromycin. Patient improved after 48 h and was discharged on oral aspirin. Conclusion Incomplete KD may co-exist with COVID-19 infection in infant. Early ...
Indian Journal of Pediatrics, 2021
Journal of Tropical Pediatrics, 2021
Background Our goal was to study the demographic, clinical and laboratory profile and outcome of ... more Background Our goal was to study the demographic, clinical and laboratory profile and outcome of scrub typhus-associated hemophagocytic lymphohistiocytosis (HLH) in the pediatric age group. Methods We conducted a prospective observational study in a tertiary care teaching hospital over a period of 1 year. Children in the age group of 1 month to 12 years with IgM ELISA positive for scrub typhus were included in the study. HLH was diagnosed using HLH-2004 criteria. Demographic, clinical and laboratory profile, treatment and outcome of HLH patients were noted and also compared with non-HLH scrub typhus patients. Results Among 58 children with scrub typhus infection, 18 had HLH. The mean age of patients with HLH was 35.3 ± 44.8 months and 61% were male. Anemia, thrombocytopenia and hyperferritinemia were seen in all the patients. Hypertriglyceridemia, hypofibrinogenemia and coagulopathy were noted in 78%, 56% and 44%, respectively. All the patients were treated with intravenous doxycycl...
Journal of Tropical Pediatrics, 2020
Dengue is a major health concern in South Asian countries transmitted by bite of day breeder mosq... more Dengue is a major health concern in South Asian countries transmitted by bite of day breeder mosquitoes Aedes aegypti and Aedes albopictus. Severity of plasma leak, shock, bleeding tendency and other organ dysfunction can be more pronounced in infants. The management becomes further complicated in the presence of a co-existing COVID-19 infection. Although COVID-19 infection is usually asymptomatic or has mild manifestations in children, however in presence of serious co-infection like dengue it can modify the course of the illness and lead to drastic consequences. Here, we present one such case of a 9-month-old female child who tested positive for dengue as well as COVID-19 during the ongoing corona pandemic and went on to develop shock, encephalopathy with deranged liver enzymes but managed to overcome all odds and recover from the disease by day 14 of illness.
Indian journal of experimental biology, 2003
Adrenal delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activity and serum corticost... more Adrenal delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activity and serum corticosterone level were significantly higher in rats fed with 5% casein or 4% albumin diets after 1 hr of ether anaesthetic stress as compared to the controls, 5% casein and 20% casein (equivalent to 4% albumin) respectively. Ether anaesthesia to 20% casein fed rats caused no change in adrenal delta5-3beta-HSD activity and serum corticosterone level when compared with controls fed 20% casein diet. The results suggest that high milk protein diet may prevent acute stress effects by protecting adrenocortical activity. The present investigation opens up a new area of management of stress.
Journal of Tropical Pediatrics, 2021
Background and objectives Assessing the co-infections with COVID-19 is crucial to delineate its t... more Background and objectives Assessing the co-infections with COVID-19 is crucial to delineate its true clinical impact. Pediatric information in this aspect is limited. Our study aims to analyze the spectrum of co-infections in pediatric COVID-19 patients and determine the clinical as well as laboratory parameters predicting co-infection. Methodology In this prospective observational study conducted from June to December 2020 in a single tertiary care institution, data pertaining to demographic, illness and treatment-related variables were analyzed among two subsets of pediatric patients of age 1 month–12 years with RT–PCR-confirmed COVID-19 infection-Group A: those with confirmed co-infection and Group B: moderate to severe disease without co-infection. Among Group A, etiology of co-infection was characterized through relevant microbiological examination within 48 h admission. Result Among our study population, 15.03% and 20.6% had co-infections and moderate to severe disease respect...
The Indian Journal of Animal Sciences
The present research work was undertaken to compare the expression of paternally imprinted (P57KI... more The present research work was undertaken to compare the expression of paternally imprinted (P57KIP2, Grb10 and Xist) and maternally imprinted gene (Dlk1) among diploid parthenogenetic (DIP), female sexed in vivo and female sexed in vitro produced embryonic cell colony. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and activated by ethanol (7%) for 5 min followed by incubation with 2 mM 6-dimethyl amino purine (DMAP) for 4 h for DIP embryos production. In vivo embryo was collected by surgical method from 10 super ovulated goats using Pluset®. In vitro embryos were produced by maturation of good quality oocyte followed by fertilization with superior male germplasm from the institute flock. Embryonic cell colony were developed from 8 to 16 and morula stage DIP, IVF and in vivo derived embryos. These colonies were used for studying the paternal and maternal imprinted genes expression. Karyotyping and sexing of embryonic cell colony was done to get onl...
Genomics
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Biological Research, 2021
Background PGF2α is essential for the induction of the corpus luteum regression which in turn red... more Background PGF2α is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid elevation of EGR1 was observed after luteolytic dose of PGF2α. EGR1 is involved in the transactivation of many genes, including TGFβ1, which plays an important role during luteal regression. Methods The current study was conducted in buffalo luteal cells with the aim to better understand the role of EGR1 in transactivation of TGFβ1 during PGF2α induced luteal regression. Luteal cells from mid stage corpus luteum of buffalo were cultured and treated with different doses of PGF2α for different time durations. Relative expression of mRNAs encoding for enzymes within the progesterone biosynthetic pathway (3βHSD, CYP11A1 and StAR); Caspase 3; AKT were analyzed to confirm the occurrence of luteol...
International Journal of Livestock Research, 2017
The present study was designed to bring out the effects of antioxidant and increased energy allow... more The present study was designed to bring out the effects of antioxidant and increased energy allowance on reproductive performance and steroids during transition period in crossbred cow. Twenty six advanced pregnant crossbred cows of parity between 2 to 4 and lactation potential of >10 L/day were divided into two equal groups (n=13 cows/group). Cows were fed diets either (i) supplemented with vitamin E (80 IU/kg DM), Selenium (Se; 0.3 mg/kg DM) and increased energy allowance in the form of 20% extra concentrate (TRT) or (ii) basal diet without any supplementation as control (CON). Vitamin E and Se were supplemented with wheat flour bolus from-4 to 8 week of calving where energy allowance increased from 2 to 8 week of calving on daily basis to individual animal (where 0 is day of calving). Blood samples were collected on weekly interval from-4 to 8 week of calving. Post-partum reproductive performance was assessed in terms of service per conception, calving to conception interval, days calving to first AI, first AI pregnancy rate and relative reproductive disturbance in both the groups. In reproductive steroids, serum level of progesterone and estradiol were estimated through radio immuno assay (RIA). Marked favorable impact over fertility was recorded in TRT group through reduced service per conception, calving to conception interval, days calving to first AI and increased first AI pregnancy rate along with relatively less reproductive disturbance. However, study does not reveal any significant effect of vitamin E, Se and increased energy allowance on reproductive steroids.
Iranian Journal of Veterinary Research, 2014
Summary The study was conducted to demonstrate expression profile of cold shock protein genes; co... more Summary The study was conducted to demonstrate expression profile of cold shock protein genes; cold inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3) in goats ( Capra hircus ) during different seasons to check the gene expression in different thermal conditions. Blood samples were collected from healthy goats of tropical and temperate region during peak winter, moderate season and peak summer. Goats were divided in three (n=6 in each group) age groups- (I) up to 2 years, (II) 2 to 5 years and (III) above 5 years. RNA isolation was done from separated peripheral blood mononuclear cells (PBMCs) by Trizol reagent. Real-time PCR was applied to investigate mRNA expression of examined factors. The mRNA expression of CIRP and RBM3 was higher in all age groups during peak winter season as compared to peak summer season in both tropical and temperate region goats. To conclude, our results demonstrated expression of CIRP and RBM3 mRNAs in caprine PBMCs and increased ...
The Pharma Innovation Journal, 2019
To investigate the effects of Vascular endothelial growth factor on progesterone (P4) synthesis a... more To investigate the effects of Vascular endothelial growth factor on progesterone (P4) synthesis and on the expression of a steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage (CYP11A1), 3-hydroxysteroid dehydrogenase(3βHSD), proliferating cell nuclear antigen (PCNA), BCL2 Associated X(BAX) and von Willebrand factor (vWF) in trophoblast cells of water buffalo. Trophoblast cell was isolated from the early pregnant uterus. Cells were treated with VEGF @ 1, 10, and 100 ng/ml for 24, 48, and 72h. RIA assessed P4, while mRNA expression by qRT-PCR. P4 secretion was maximum (P<0.05) at the highest dose maintained for a longer duration. The mRNA expression of StAR, CYP11A1, 3βHSD, PCNA and vWF were upregulated (P<0.05) at 48h and 72h at the highest dose. Expression of BAX was downregulated (P<0.001) at 72h. In conclusion, VEGF modulates the TCC on steroidogenesis, cell survivability, and angiogenesis in the placenta through an autocrine and...
International Journal of Current Microbiology and Applied Sciences, 2020
The climate change is pertinent and its impact on the animal and human population is tremendous, ... more The climate change is pertinent and its impact on the animal and human population is tremendous, directly threatens food security of all living beings. The decreased availability of forages, feed intake and feed conversion efficiency in domestic animals and the increased ingestion three times will be the major impacts of climate change (RojasDowning et al., 2017). The production parameters like milk and meat productivity have been adversely affected by the extreme climate scenario. The adverse effect of increased temperature on milk production (Henry et al., 2012; Nardone et al., 2010; Thornton et al., 2009) has been scaled about 15 million tonnes by 2050 (Venkateswarlu, and Rao, 2013).
Scientific Reports, 2020
BMPs are multifunctional growth factors implicated in regulating the ovarian function as key intr... more BMPs are multifunctional growth factors implicated in regulating the ovarian function as key intra-ovarian factors. Biological effects of BMPs are mediated through binding with membrane bound receptors like BMPR-IB and initiating downstream Smad signaling pathway. FecB mutation, regarded as a loss of function mutation in the BMPR-IB gene was identified in certain sheep breeds having high fecundity. Similar type of fecundity genes in goats have not been discovered so far. Hence, the current study was designed to investigate the effects of BMPR-IB gene modulation on granulosa cell function in goats. The BMPR-IB gene was knocked out using CRISPR-Cas technology in granulosa cells and cultured in vitro with BMP-4 stimulation for three different durations In addition, the FecB mutation was introduced in the BMPR-IB gene applying Easi-CRISPR followed by BMP-4/7 stimulation for 72 h. Steroidogenesis and cell viability were studied to explore the granulosa cell function on BMPR-IB gene modul...
Journal of Applied Animal Research, 2020
Our current investigation was aimed to study differential influence of heat stress on defensive f... more Our current investigation was aimed to study differential influence of heat stress on defensive functions of blood polymorphonuclear cells among native and crossbred cows by culturing them in vitro after animal exposure to in vivo heat stress. Blood was collected from 18 cows which were divided in to three groups based on their breed as, group I (Sahiwal), group II (Tharparkar) and group III (Crossbred). THI was calculated by temperature and relative humidity (RH). PMN were isolated and were incubated at 38°C. Phagocytosis and lymphocyte proliferation was then investigated by NBT and MTT assays, respectively. The overall mean THI was significantly (p < 0.5) higher during summer (88.41 ± 1.54) when compared to the winter season (64.75 ± 0.97). There is a significant high lymphocytic proliferation in Group II when compared to Group III cattle. Also, there is a significant decrease in PA during the summer when compared to winter in all the breeds and in group III there is significant decrease in LPA during summer. The results of the present study suggest that indigenous cattle Tharparkar exhibited more tolerance to heat stress than the Group-I and Group-III during summer. However, in winter, all the breeds showed similar.
Veterinary World, 2018
The aim of this study was to evaluate the relationship of scrotal circumference (SC) with plasma ... more The aim of this study was to evaluate the relationship of scrotal circumference (SC) with plasma testosterone, seminal vesicles (SVs) weight, and its secretion as measurable indicators of fertility and also to sequence and establish phylogenetic relatedness of certain SV protein genes with other species as such integrated approach is lacking. Materials and Methods: Altogether, 59 apparently healthy male buffaloes sacrificed at slaughterhouse were selected (irrespective of breed) for measuring SC and collecting blood and paired SVs. The SC was measured at greater curvature using soft thread. In the present study, blood plasma testosterone, cholesterol, protein, and glucose in addition to SV fructose, citric acid and proteins in SV fluid were also estimated. The SV tissue was fixed in RNAlater for RNA extraction.Male buffaloes were categorized as per total SV weight into Group I (<5.0 g), Group II (5.0-7.84 g), and Group III (>8.0 g) and dentitions-I (≤18 months), II (18-24 months), and III (≥24 months) to assess the effect of weight and dentition age on SC, SV weight, and its certain secretions. Data were analyzed using linear model procedure including Tukey HSD test and Pearson's correlation coefficient. Variance inflation and condition index were also used to assess multicollinearity. Results: Gross and histomorphological evaluation of SVs did not show any abnormality. Macronutrients (plasma protein, glucose, and cholesterol) showed non-significant (p>0.05) variation between groups. The SC and SV weight varied significantly (p<0.05) with a significant positive relationship with plasma testosterone, SV protein, fructose, and citric acid. In addition, testosterone concentration also showed increasing trend from Groups I to III but increased significantly (p<0.05) from Group II to III with positive and significant correlations with SV protein, fructose, and citric acid similar to SV weight and SC. Binders of sperm protein (BSP1, 3, and 5) genes (full length) were sequenced and established an evolutionary relationship which is lacking in buffalo. Conclusion: The present findings established a significant positive correlation of SC with that of other fertility parameters related to SVs weight and its secretions: Fructose, citric acid, and protein (inclusive of BSPs sequenced full length), and testosterone. Therefore, the present integrated approach along with certain semen quality attributes reflecting epididymis function could be used as a predictive fertility marker for grading and selection of breeding bulls and their progenies to develop outstanding bull mother farm.
Indian Journal of Critical Care Medicine
Background: To evaluate the role of ultrasound during initial fluid resuscitation along with clin... more Background: To evaluate the role of ultrasound during initial fluid resuscitation along with clinical guidance in reducing the incidence of fluid overload on day 3 in children with septic shock. Materials and methods: It was a prospective, parallel limb open-labeled randomized controlled superiority trial done in the PICU of a governmentaided tertiary care hospital in Eastern India. Patient enrolment took place between June 2021 and March 2022. Fifty-six children aged between 1 month and 12 years, with proven or suspected septic shock, were randomized to receive either ultrasound-guided or clinically guided fluid boluses (1:1 ratio) and subsequently followed up for various outcomes. The primary outcome was frequency of fluid overload on day 3 of admission. The treatment group received ultrasound-guided fluid boluses along with the clinical guidance and the control group received the same but without ultrasound guidance upto 60 mL/kg of fluid boluses. Results: The frequency of fluid overload on day 3 of admission was significantly lower in the ultrasound group (25% vs 62%, p = 0.012) as was the median (IQR) cumulative fluid balance percentage on day 3 [6.5 (3.3-10.3) vs 11.3 (5.4-17.5), p = 0.02]. The amount of fluid bolus administered was also significantly lower by ultrasound [median 40 (30-50) vs 50 (40-80) mL/kg, p = 0.003]. Resuscitation time was shorter in the ultrasound group (13.4 ± 5.6 vs 20.5 ± 8 h, p = 0.002). Conclusion: Ultrasound-guided fluid boluses were found to be significantly better than clinically guided therapy, in preventing fluid overload and its associated complications in children with septic shock. These factors make ultrasound a potentially useful tool for resuscitation of children with septic shock in the PICU.
Indian Pediatrics
Objective: To determine the clinical presentation and outcome of children infected with severe ac... more Objective: To determine the clinical presentation and outcome of children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the third wave of the pandemic in India. Method: A review of hospital records was done at a tertiary care hospital, for children seen between 1 and 25 January, 2022. Result: Out of total 112 SARS-CoV-2 positive patients, 17 were hospitalized and 95 were treated in the outpatient department. Among non-hospitalized children, fever was the commonest feature (72, 75.7%), followed by sneezing, and loss of appetite. The median (IQR) duration of symptoms was 2.5 (1.5) days. Among hospitalized children, 10 had co-morbidities and one-third required intensive care unit admission. MIS-C was diagnosed in four patients. Out of 4 mechanically ventilated patients, two had coronavirus disease (COVID) pneumonia. The mean (SD) length of hospital stay was 7.5 (2.5) days. One child with leukemia died during management. Conclusion: During the third wave of the pandemic, most children had symptomatic illnesses, but recovery was fast among non-hospitalized children.
Indian Journal of Pediatrics
Objective To determine the outcomes in children with MIS-C receiving different immunomodulatory t... more Objective To determine the outcomes in children with MIS-C receiving different immunomodulatory treatment. Methods In this multicentric, retrospective cohort study, data regarding treatment and outcomes of children meeting the WHO case definition for MIS-C, were collected. The primary composite outcome was the requirement of vasoactive/inotropic support on day 2 or beyond or need of mechanical ventilation on day 2 or beyond after initiation of immunomodulatory treatment or death during hospitalization in the treatment groups. Logistic regression and propensity score matching analyses were used to compare the outcomes in different treatment arms based on the initial immunomodulation, i.e., IVIG alone, IVIG plus steroids, and steroids alone. Results The data of 368 children (diagnosed between April 2020 and June 2021) meeting the WHO case definition for MIS-C, were analyzed. Of the 368 subjects, 28 received IVIG alone, 82 received steroids alone, 237 received IVIG and steroids, and 21 did not receive any immunomodulation. One hundred fifty-six (42.39%) children had the primary outcome. On logistic regression analysis, the treatment group was not associated with the primary outcome; only the children with shock at diagnosis had higher odds for the occurrence of the outcome [OR (95% CI): 11.4 (5.19-25.0), p < 0.001]. On propensity score matching analysis, the primary outcome was comparable in steroid (n = 45), and IVIG plus steroid (n = 84) groups (p = 0.515). Conclusion While no significant difference was observed in the frequency of occurrence of the primary outcome in different treatment groups, data from adequately powered RCTs are required for definitive recommendations.
IP Journal of Paediatrics and Nursing Science
Parents of the COVID -19 positive hospitalized children are facing different psychological stress... more Parents of the COVID -19 positive hospitalized children are facing different psychological stresses which are affecting child’s care and well-being. To determine the psychological stress, anxiety and depression and to identify the associated influencing factors in mothers of hospitalized COVID-19 infected children In this cross-sectional observational study, 150 mothers of COVID-19 positive hospitalized children in a paediatric tertiary care hospital were randomly selected and studied. Data collection tool was a two part questionnaire; the first part was based on ‘Perceived stress scale-10’ and ‘Hospital anxiety and depression scale’ and the second part included stressors related to COVID-19 pandemic selected based on previous researches. The incidence of these stressors and their significance in causing stress, anxiety and depression was assessed using standard statistical methods.83.4% had moderate psychological stress, 35% had significant anxiety and 38% had significant depressio...
Journal of Tropical Pediatrics, 2020
Background Recently a severe form of COVID-19 infection has been described in a cluster of childr... more Background Recently a severe form of COVID-19 infection has been described in a cluster of children presenting as multisystem inflammatory condition. One of the important spectrum of this condition is incomplete Kawasaki disease (KD). Case report A 5-month-old male child presented with high-spiking fever for 5 days with skin rash, bilateral non-purulent conjunctivitis and irritability. His C-reactive protein was markedly elevated (215.4 mg/l). Echocardiography revealed dilated left main coronary artery (3.0 mm, Z score +4.30) and left anterior descending artery (2.37 mm, Z score +3.76). Concomitantly Reverse Transcription- Polymerase Chain Reaction for COVID-19 was positive on fifth day sample. He was diagnosed as incomplete KD with COVID-19 infection and treated with intravenous immunoglobulin (IVIG) (2 g/kg), oral aspirin and azithromycin. Patient improved after 48 h and was discharged on oral aspirin. Conclusion Incomplete KD may co-exist with COVID-19 infection in infant. Early ...
Indian Journal of Pediatrics, 2021
Journal of Tropical Pediatrics, 2021
Background Our goal was to study the demographic, clinical and laboratory profile and outcome of ... more Background Our goal was to study the demographic, clinical and laboratory profile and outcome of scrub typhus-associated hemophagocytic lymphohistiocytosis (HLH) in the pediatric age group. Methods We conducted a prospective observational study in a tertiary care teaching hospital over a period of 1 year. Children in the age group of 1 month to 12 years with IgM ELISA positive for scrub typhus were included in the study. HLH was diagnosed using HLH-2004 criteria. Demographic, clinical and laboratory profile, treatment and outcome of HLH patients were noted and also compared with non-HLH scrub typhus patients. Results Among 58 children with scrub typhus infection, 18 had HLH. The mean age of patients with HLH was 35.3 ± 44.8 months and 61% were male. Anemia, thrombocytopenia and hyperferritinemia were seen in all the patients. Hypertriglyceridemia, hypofibrinogenemia and coagulopathy were noted in 78%, 56% and 44%, respectively. All the patients were treated with intravenous doxycycl...
Journal of Tropical Pediatrics, 2020
Dengue is a major health concern in South Asian countries transmitted by bite of day breeder mosq... more Dengue is a major health concern in South Asian countries transmitted by bite of day breeder mosquitoes Aedes aegypti and Aedes albopictus. Severity of plasma leak, shock, bleeding tendency and other organ dysfunction can be more pronounced in infants. The management becomes further complicated in the presence of a co-existing COVID-19 infection. Although COVID-19 infection is usually asymptomatic or has mild manifestations in children, however in presence of serious co-infection like dengue it can modify the course of the illness and lead to drastic consequences. Here, we present one such case of a 9-month-old female child who tested positive for dengue as well as COVID-19 during the ongoing corona pandemic and went on to develop shock, encephalopathy with deranged liver enzymes but managed to overcome all odds and recover from the disease by day 14 of illness.
Indian journal of experimental biology, 2003
Adrenal delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activity and serum corticost... more Adrenal delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activity and serum corticosterone level were significantly higher in rats fed with 5% casein or 4% albumin diets after 1 hr of ether anaesthetic stress as compared to the controls, 5% casein and 20% casein (equivalent to 4% albumin) respectively. Ether anaesthesia to 20% casein fed rats caused no change in adrenal delta5-3beta-HSD activity and serum corticosterone level when compared with controls fed 20% casein diet. The results suggest that high milk protein diet may prevent acute stress effects by protecting adrenocortical activity. The present investigation opens up a new area of management of stress.
Journal of Tropical Pediatrics, 2021
Background and objectives Assessing the co-infections with COVID-19 is crucial to delineate its t... more Background and objectives Assessing the co-infections with COVID-19 is crucial to delineate its true clinical impact. Pediatric information in this aspect is limited. Our study aims to analyze the spectrum of co-infections in pediatric COVID-19 patients and determine the clinical as well as laboratory parameters predicting co-infection. Methodology In this prospective observational study conducted from June to December 2020 in a single tertiary care institution, data pertaining to demographic, illness and treatment-related variables were analyzed among two subsets of pediatric patients of age 1 month–12 years with RT–PCR-confirmed COVID-19 infection-Group A: those with confirmed co-infection and Group B: moderate to severe disease without co-infection. Among Group A, etiology of co-infection was characterized through relevant microbiological examination within 48 h admission. Result Among our study population, 15.03% and 20.6% had co-infections and moderate to severe disease respect...
The Indian Journal of Animal Sciences
The present research work was undertaken to compare the expression of paternally imprinted (P57KI... more The present research work was undertaken to compare the expression of paternally imprinted (P57KIP2, Grb10 and Xist) and maternally imprinted gene (Dlk1) among diploid parthenogenetic (DIP), female sexed in vivo and female sexed in vitro produced embryonic cell colony. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and activated by ethanol (7%) for 5 min followed by incubation with 2 mM 6-dimethyl amino purine (DMAP) for 4 h for DIP embryos production. In vivo embryo was collected by surgical method from 10 super ovulated goats using Pluset®. In vitro embryos were produced by maturation of good quality oocyte followed by fertilization with superior male germplasm from the institute flock. Embryonic cell colony were developed from 8 to 16 and morula stage DIP, IVF and in vivo derived embryos. These colonies were used for studying the paternal and maternal imprinted genes expression. Karyotyping and sexing of embryonic cell colony was done to get onl...
Genomics
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Biological Research, 2021
Background PGF2α is essential for the induction of the corpus luteum regression which in turn red... more Background PGF2α is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid elevation of EGR1 was observed after luteolytic dose of PGF2α. EGR1 is involved in the transactivation of many genes, including TGFβ1, which plays an important role during luteal regression. Methods The current study was conducted in buffalo luteal cells with the aim to better understand the role of EGR1 in transactivation of TGFβ1 during PGF2α induced luteal regression. Luteal cells from mid stage corpus luteum of buffalo were cultured and treated with different doses of PGF2α for different time durations. Relative expression of mRNAs encoding for enzymes within the progesterone biosynthetic pathway (3βHSD, CYP11A1 and StAR); Caspase 3; AKT were analyzed to confirm the occurrence of luteol...
International Journal of Livestock Research, 2017
The present study was designed to bring out the effects of antioxidant and increased energy allow... more The present study was designed to bring out the effects of antioxidant and increased energy allowance on reproductive performance and steroids during transition period in crossbred cow. Twenty six advanced pregnant crossbred cows of parity between 2 to 4 and lactation potential of >10 L/day were divided into two equal groups (n=13 cows/group). Cows were fed diets either (i) supplemented with vitamin E (80 IU/kg DM), Selenium (Se; 0.3 mg/kg DM) and increased energy allowance in the form of 20% extra concentrate (TRT) or (ii) basal diet without any supplementation as control (CON). Vitamin E and Se were supplemented with wheat flour bolus from-4 to 8 week of calving where energy allowance increased from 2 to 8 week of calving on daily basis to individual animal (where 0 is day of calving). Blood samples were collected on weekly interval from-4 to 8 week of calving. Post-partum reproductive performance was assessed in terms of service per conception, calving to conception interval, days calving to first AI, first AI pregnancy rate and relative reproductive disturbance in both the groups. In reproductive steroids, serum level of progesterone and estradiol were estimated through radio immuno assay (RIA). Marked favorable impact over fertility was recorded in TRT group through reduced service per conception, calving to conception interval, days calving to first AI and increased first AI pregnancy rate along with relatively less reproductive disturbance. However, study does not reveal any significant effect of vitamin E, Se and increased energy allowance on reproductive steroids.
Iranian Journal of Veterinary Research, 2014
Summary The study was conducted to demonstrate expression profile of cold shock protein genes; co... more Summary The study was conducted to demonstrate expression profile of cold shock protein genes; cold inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3) in goats ( Capra hircus ) during different seasons to check the gene expression in different thermal conditions. Blood samples were collected from healthy goats of tropical and temperate region during peak winter, moderate season and peak summer. Goats were divided in three (n=6 in each group) age groups- (I) up to 2 years, (II) 2 to 5 years and (III) above 5 years. RNA isolation was done from separated peripheral blood mononuclear cells (PBMCs) by Trizol reagent. Real-time PCR was applied to investigate mRNA expression of examined factors. The mRNA expression of CIRP and RBM3 was higher in all age groups during peak winter season as compared to peak summer season in both tropical and temperate region goats. To conclude, our results demonstrated expression of CIRP and RBM3 mRNAs in caprine PBMCs and increased ...
The Pharma Innovation Journal, 2019
To investigate the effects of Vascular endothelial growth factor on progesterone (P4) synthesis a... more To investigate the effects of Vascular endothelial growth factor on progesterone (P4) synthesis and on the expression of a steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage (CYP11A1), 3-hydroxysteroid dehydrogenase(3βHSD), proliferating cell nuclear antigen (PCNA), BCL2 Associated X(BAX) and von Willebrand factor (vWF) in trophoblast cells of water buffalo. Trophoblast cell was isolated from the early pregnant uterus. Cells were treated with VEGF @ 1, 10, and 100 ng/ml for 24, 48, and 72h. RIA assessed P4, while mRNA expression by qRT-PCR. P4 secretion was maximum (P<0.05) at the highest dose maintained for a longer duration. The mRNA expression of StAR, CYP11A1, 3βHSD, PCNA and vWF were upregulated (P<0.05) at 48h and 72h at the highest dose. Expression of BAX was downregulated (P<0.001) at 72h. In conclusion, VEGF modulates the TCC on steroidogenesis, cell survivability, and angiogenesis in the placenta through an autocrine and...
International Journal of Current Microbiology and Applied Sciences, 2020
The climate change is pertinent and its impact on the animal and human population is tremendous, ... more The climate change is pertinent and its impact on the animal and human population is tremendous, directly threatens food security of all living beings. The decreased availability of forages, feed intake and feed conversion efficiency in domestic animals and the increased ingestion three times will be the major impacts of climate change (RojasDowning et al., 2017). The production parameters like milk and meat productivity have been adversely affected by the extreme climate scenario. The adverse effect of increased temperature on milk production (Henry et al., 2012; Nardone et al., 2010; Thornton et al., 2009) has been scaled about 15 million tonnes by 2050 (Venkateswarlu, and Rao, 2013).
Scientific Reports, 2020
BMPs are multifunctional growth factors implicated in regulating the ovarian function as key intr... more BMPs are multifunctional growth factors implicated in regulating the ovarian function as key intra-ovarian factors. Biological effects of BMPs are mediated through binding with membrane bound receptors like BMPR-IB and initiating downstream Smad signaling pathway. FecB mutation, regarded as a loss of function mutation in the BMPR-IB gene was identified in certain sheep breeds having high fecundity. Similar type of fecundity genes in goats have not been discovered so far. Hence, the current study was designed to investigate the effects of BMPR-IB gene modulation on granulosa cell function in goats. The BMPR-IB gene was knocked out using CRISPR-Cas technology in granulosa cells and cultured in vitro with BMP-4 stimulation for three different durations In addition, the FecB mutation was introduced in the BMPR-IB gene applying Easi-CRISPR followed by BMP-4/7 stimulation for 72 h. Steroidogenesis and cell viability were studied to explore the granulosa cell function on BMPR-IB gene modul...
Journal of Applied Animal Research, 2020
Our current investigation was aimed to study differential influence of heat stress on defensive f... more Our current investigation was aimed to study differential influence of heat stress on defensive functions of blood polymorphonuclear cells among native and crossbred cows by culturing them in vitro after animal exposure to in vivo heat stress. Blood was collected from 18 cows which were divided in to three groups based on their breed as, group I (Sahiwal), group II (Tharparkar) and group III (Crossbred). THI was calculated by temperature and relative humidity (RH). PMN were isolated and were incubated at 38°C. Phagocytosis and lymphocyte proliferation was then investigated by NBT and MTT assays, respectively. The overall mean THI was significantly (p < 0.5) higher during summer (88.41 ± 1.54) when compared to the winter season (64.75 ± 0.97). There is a significant high lymphocytic proliferation in Group II when compared to Group III cattle. Also, there is a significant decrease in PA during the summer when compared to winter in all the breeds and in group III there is significant decrease in LPA during summer. The results of the present study suggest that indigenous cattle Tharparkar exhibited more tolerance to heat stress than the Group-I and Group-III during summer. However, in winter, all the breeds showed similar.
Veterinary World, 2018
The aim of this study was to evaluate the relationship of scrotal circumference (SC) with plasma ... more The aim of this study was to evaluate the relationship of scrotal circumference (SC) with plasma testosterone, seminal vesicles (SVs) weight, and its secretion as measurable indicators of fertility and also to sequence and establish phylogenetic relatedness of certain SV protein genes with other species as such integrated approach is lacking. Materials and Methods: Altogether, 59 apparently healthy male buffaloes sacrificed at slaughterhouse were selected (irrespective of breed) for measuring SC and collecting blood and paired SVs. The SC was measured at greater curvature using soft thread. In the present study, blood plasma testosterone, cholesterol, protein, and glucose in addition to SV fructose, citric acid and proteins in SV fluid were also estimated. The SV tissue was fixed in RNAlater for RNA extraction.Male buffaloes were categorized as per total SV weight into Group I (<5.0 g), Group II (5.0-7.84 g), and Group III (>8.0 g) and dentitions-I (≤18 months), II (18-24 months), and III (≥24 months) to assess the effect of weight and dentition age on SC, SV weight, and its certain secretions. Data were analyzed using linear model procedure including Tukey HSD test and Pearson's correlation coefficient. Variance inflation and condition index were also used to assess multicollinearity. Results: Gross and histomorphological evaluation of SVs did not show any abnormality. Macronutrients (plasma protein, glucose, and cholesterol) showed non-significant (p>0.05) variation between groups. The SC and SV weight varied significantly (p<0.05) with a significant positive relationship with plasma testosterone, SV protein, fructose, and citric acid. In addition, testosterone concentration also showed increasing trend from Groups I to III but increased significantly (p<0.05) from Group II to III with positive and significant correlations with SV protein, fructose, and citric acid similar to SV weight and SC. Binders of sperm protein (BSP1, 3, and 5) genes (full length) were sequenced and established an evolutionary relationship which is lacking in buffalo. Conclusion: The present findings established a significant positive correlation of SC with that of other fertility parameters related to SVs weight and its secretions: Fructose, citric acid, and protein (inclusive of BSPs sequenced full length), and testosterone. Therefore, the present integrated approach along with certain semen quality attributes reflecting epididymis function could be used as a predictive fertility marker for grading and selection of breeding bulls and their progenies to develop outstanding bull mother farm.