Savitree Limtong - Academia.edu (original) (raw)

Papers by Savitree Limtong

Research paper thumbnail of Assessment of yeasts in tropical peat swamp forests in Thailand

Mycological Progress

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Fuel Ethanol Production from Sugarcane

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

Microorganisms

During ethanol fermentation, yeast cells are exposed to various stresses that have negative effec... more During ethanol fermentation, yeast cells are exposed to various stresses that have negative effects on cell growth, cell survival, and fermentation ability. This study, therefore, aims to develop Kluyveromyces marxianus-adapted strains that are multi-stress tolerant and to increase ethanol production at high temperatures through a novel evolutionary adaptation procedure. K. marxianus DMKU 3-1042 was subjected to repetitive long-term cultivation with gradual increases in temperature (RLCGT), which exposed cells to various stresses, including high temperatures. In each cultivation step, 1% of the previous culture was inoculated into a medium containing 1% yeast extract, 2% peptone, and 2% glucose, and cultivation was performed under a shaking condition. Four adapted strains showed increased tolerance to ethanol, furfural, hydroxymethylfurfural, and vanillin, and they also showed higher production of ethanol in a medium containing 16% glucose at high temperatures. One showed stronger e...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 発酵生産性に優れた酵母Kluyveromyces marxianusの遺伝学的基盤：完全ゲノムとトランスクリプトーム解析

日本分子生物学会/日本生化学会, Nov 2, 2015

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Antagonistic Interactions between Trichoderma harzianum and Lentinula edodes

Mushroom science and biotechnology, 2002

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis

Energies, 2022

Candida tropicalis, a xylose-fermenting yeast, has the potential for converting cellulosic biomas... more Candida tropicalis, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant C. tropicalis X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in temperature (RLCGT) in the presence of a high concentration of glucose, which exposed cells to various stresses in addition to the high concentration of glucose and high temperatures. The resultant adapted strain demonstrated increased tolerance to ethanol, furfural and hydroxymethylfurfural at high temperatures and displayed improvement in fermentation ability at high glucose concentrations and xylose-fermenting ability. Transcriptome analysis revealed the up-regulation of a gene for a glucose transporter of the major facilitator superfamily and genes for stress response and cell wall proteins. Additionally, hydropathy analysis revealed that three genes for putative membrane proteins with multiple membrane-spanning segments were also u...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of High-lysine feed supplement from cassava

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Ethanol fermentation by flocculent yeasts

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Diversity of Fungi in Mangrove Forest

Soils, water, leaves and twigs were sampled from 5 different areas in mangrove forest at the Rano... more Soils, water, leaves and twigs were sampled from 5 different areas in mangrove forest at the Ranong Biosphere Reserve during July 2000 and February, April and July 2001. The salinity of water varied among different areas, but pH remained the same in all water samples. The temperature of water was highest in April. The numbers of fungi at surface layer of the soils close to the water were 1.0∞1026.3∞10 3 CFU/g and of submerged soils were 2.8∞10-1.6∞10 3 CFU/g. The numbers of fungi in surface water were 1-4∞10 CFU/ml and that at 1 meter below water surface were 1-5.6∞10 CFU/ml. The numbers of fungi from leaves were 2∞10 2 -8.3∞10 3 CFU/ml whereas those from twigs were 3.0∞10 2 -2.1∞10 4 CFU/ g. The numbers of fungi from twigs were higher than those of leaves, soils and water. The numbers of fungi in water were lowest. The fungi were isolated and identified into 16 genera 74 species. Three species, namely Mucor sp., Rhizopus sp., Syncephalastum sp. were in the Phylum Zygomycota; one, n...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Construction of xylose fermenting acid tolerant Saccharomyces cerevisiae for ethanol production from lignocellulosic agricultural waste

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Trichoderma harzianumとLentinula edodesの間の桔抗作用について

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Bioethanol Production from Sugar Cane Syrup by Thermo-tolerant Yeast, Kluyveromyces marxianus DMKU3-1042, using Fed-batch and Repeated-batch Fermentation in a Nonsterile System

Kasetsart Journal. Natural Sciences, 2012

Ethanol production from sugar cane syrup was carried out to compare the effi ciency of batch-, fe... more Ethanol production from sugar cane syrup was carried out to compare the effi ciency of batch-, fed-batch and repeated-batch fermentation using Kluyveromyces marxianus DMKU3-1042. The sugar cane syrup medium contained 18% total sugar, 0.1% (NH4)2SO4, 0.1% KH2PO4, and 0.1% MgSO4.7H2O, with the pH adjusted to 4.5. Experiments were carried out in a 2.5 L jar fermenter at a controlled temperature of 35 °C and an agitation speed of 300 rpm without aeration. All experiments were carried out in a nonsterile system that resembled the process used in the bioethanol fermentation industry in Thailand. The ethanol concentration from batch fermentation was lowest among the three processes where 6.35% w/v was achieved in 72 hr resulting in an ethanol productivity of 0.88 g.L -1 . hr -1 and the ethanol yield corresponded to 82.73% of the theoretical yield. For fed-batch fermentation, the exponential feeding scheme barely improved the ethanol yields while sigmoidal feeding gave a considerably higher...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 3Ep06 Isolation, Selection and Characterization of Thermotolerant Yeasts for Ethanol Production

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Comparison of glucose repression between thermotolerant yeast Kluyveromyces marxianus and Saccharomyces cerevisiae

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Yeast diversity in Huay Kar Kang wildlife sanctuary

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 2Dp03 Yeasts on Agronomic Crop Phylloplane : Description of Novel Species and Potential in Agricultural Application

Bookmarks Related papers MentionsView impact

Research paper thumbnail of An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand

Journal of Fungi, 2021

Ascomycetous yeast species in the genus Wickerhamomyces (Saccharomycetales, Wickerhamomycetaceae)... more Ascomycetous yeast species in the genus Wickerhamomyces (Saccharomycetales, Wickerhamomycetaceae) are isolated from various habitats and distributed throughout the world. Prior to this study, 35 species had been validly published and accepted into this genus. Beneficially, Wickerhamomyces species have been used in a number of biotechnologically applications of environment, food, beverage industries, biofuel, medicine and agriculture. However, in some studies, Wickerhamomyces species have been identified as an opportunistic human pathogen. Through an overview of diversity, taxonomy and recently published literature, we have updated a brief review of Wickerhamomyces. Moreover, two new Wickerhamomyces species were isolated from the soil samples of Assam tea (Camellia sinensis var. assamica) that were collected from plantations in northern Thailand. Herein, we have identified these species as W. lannaensis and W. nanensis. The identification of these species was based on phenotypic (mor...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effects of temperature and relative humidity on Aflatoxin B1 reduction in corn grains and antagonistic activities against Aflatoxin-producing Aspergillus flavus by a volatile organic compound-producing yeast, Kwoniella heveanensis DMKU-CE82

BioControl, 2021

As shown in our previous study, Kwoniella heveanensis DMKU-CE82, a volatile organic compound (VOC... more As shown in our previous study, Kwoniella heveanensis DMKU-CE82, a volatile organic compound (VOC)-producing yeast, demonstrated promising antagonistic activity against aflatoxin-producing strain of Aspergillus flavus . This yeast’s volatile organic compounds (VOCs) could reduce Aflatoxin B1 (AFB1) in corn grains. In the current study, we evaluated the effect of temperatures and relative humidity on AFB1 reduction during grain storage when co-incubated with this VOC-producing yeast. The VOCs produced by K. heveanensis DMKU-CE82 could promote reduction of AFB1 to less than 20 part per billion (ppb) in the fungal contaminated corn grains under most storage conditions at 35 °C. The major VOCs produced by 2-, 4-, and 6-day-old yeast cultures were closely matched to 3-methyl-1-butanol, 2-methyl-1-butanol, hydrazine-1-1-dimethyl, and butanoic acid-3-methyl. In addition, this yeast strain had the ability to produce β-1,3-glucanase, amylase, cellulase, chitinase, siderophores, and biofilms. Scanning electron microscopy also confirmed the antagonistic activity of K. heveanensis DMKU-CE82 as it caused structural damage to conidia and inhibited the development of mycelia and conidiophores in both direct fungal–yeast interaction and the VOC method in corn grains. These results demonstrated that this yeast strain could be a promising biocontrol agent against aflatoxin-producing fungi in agricultural products.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Phylloplane Yeasts in Tropical Climates

Yeasts in Natural Ecosystems: Diversity, 2017

Phylloplane, which refers to the surfaces of aboveground parts of plants, includes mainly leaves ... more Phylloplane, which refers to the surfaces of aboveground parts of plants, includes mainly leaves and has been recognized as an important habitat for microorganisms. The growth of phylloplane microorganisms is dependent on organic and inorganic substances on the leaf surfaces that are either secreted by the plant or originate from external sources. Several factors that structure the microbial communities in phylloplane such as leaf age, plant species, growing conditions, environmental factors, geography and competing microorganisms have been reported. To date, the diversity of microorganisms in the phylloplane has been studied intensively. However, only a small number of articles have focused on yeasts. In this chapter, we review the methodologies, both culture-dependent and culture-independent methods, which have been utilized for assessment of leaf-associated yeasts. The diversity of phylloplane yeasts in the tropical regions assessed by those techniques is discussed. In the course of investigations, phylloplane yeasts in tropical countries have been shown to be highly diverse. Many novel yeast species have been discovered by both culture-dependent and culture-independent methods. These studies suggest that extensive studies of phylloplane yeasts from tropical regions will lead to recovering a so far underappreciated diversity.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae

International Journal of Systematic and Evolutionary Microbiology, 2020

Seven yeast strains, DMKU VGT1-14T, DMKU VGT1-19T, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DM... more Seven yeast strains, DMKU VGT1-14T, DMKU VGT1-19T, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DMKU VGT2-19 and DMKU VGT6-14, were isolated from a grease trap in Thailand and two strains, SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, these strains represented two novel yeast species of the genus Wickerhamiella. In terms of pairwise sequence similarity, four strains, DMKU VGT1-14, DMKU-JMGT1-32, DMKU VGT6-14 and SN-102, were closely related to Wickerhamiella infanticola NRRL Y-17858T but differed by 13 nucleotide substitutions with one gap (2.46 %) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2 %) in the ITS region. The strains DMKU VGT1-19T, DMKU-JMGT1-28, DMKU VGT2-06, DMKU VGT2-19 and SJ-1, differed from the type strain of the most closely re...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Assessment of yeasts in tropical peat swamp forests in Thailand

Mycological Progress

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Fuel Ethanol Production from Sugarcane

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

Microorganisms

During ethanol fermentation, yeast cells are exposed to various stresses that have negative effec... more During ethanol fermentation, yeast cells are exposed to various stresses that have negative effects on cell growth, cell survival, and fermentation ability. This study, therefore, aims to develop Kluyveromyces marxianus-adapted strains that are multi-stress tolerant and to increase ethanol production at high temperatures through a novel evolutionary adaptation procedure. K. marxianus DMKU 3-1042 was subjected to repetitive long-term cultivation with gradual increases in temperature (RLCGT), which exposed cells to various stresses, including high temperatures. In each cultivation step, 1% of the previous culture was inoculated into a medium containing 1% yeast extract, 2% peptone, and 2% glucose, and cultivation was performed under a shaking condition. Four adapted strains showed increased tolerance to ethanol, furfural, hydroxymethylfurfural, and vanillin, and they also showed higher production of ethanol in a medium containing 16% glucose at high temperatures. One showed stronger e...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 発酵生産性に優れた酵母Kluyveromyces marxianusの遺伝学的基盤：完全ゲノムとトランスクリプトーム解析

日本分子生物学会/日本生化学会, Nov 2, 2015

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Antagonistic Interactions between Trichoderma harzianum and Lentinula edodes

Mushroom science and biotechnology, 2002

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis

Energies, 2022

Candida tropicalis, a xylose-fermenting yeast, has the potential for converting cellulosic biomas... more Candida tropicalis, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant C. tropicalis X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in temperature (RLCGT) in the presence of a high concentration of glucose, which exposed cells to various stresses in addition to the high concentration of glucose and high temperatures. The resultant adapted strain demonstrated increased tolerance to ethanol, furfural and hydroxymethylfurfural at high temperatures and displayed improvement in fermentation ability at high glucose concentrations and xylose-fermenting ability. Transcriptome analysis revealed the up-regulation of a gene for a glucose transporter of the major facilitator superfamily and genes for stress response and cell wall proteins. Additionally, hydropathy analysis revealed that three genes for putative membrane proteins with multiple membrane-spanning segments were also u...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of High-lysine feed supplement from cassava

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Ethanol fermentation by flocculent yeasts

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Diversity of Fungi in Mangrove Forest

Soils, water, leaves and twigs were sampled from 5 different areas in mangrove forest at the Rano... more Soils, water, leaves and twigs were sampled from 5 different areas in mangrove forest at the Ranong Biosphere Reserve during July 2000 and February, April and July 2001. The salinity of water varied among different areas, but pH remained the same in all water samples. The temperature of water was highest in April. The numbers of fungi at surface layer of the soils close to the water were 1.0∞1026.3∞10 3 CFU/g and of submerged soils were 2.8∞10-1.6∞10 3 CFU/g. The numbers of fungi in surface water were 1-4∞10 CFU/ml and that at 1 meter below water surface were 1-5.6∞10 CFU/ml. The numbers of fungi from leaves were 2∞10 2 -8.3∞10 3 CFU/ml whereas those from twigs were 3.0∞10 2 -2.1∞10 4 CFU/ g. The numbers of fungi from twigs were higher than those of leaves, soils and water. The numbers of fungi in water were lowest. The fungi were isolated and identified into 16 genera 74 species. Three species, namely Mucor sp., Rhizopus sp., Syncephalastum sp. were in the Phylum Zygomycota; one, n...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Construction of xylose fermenting acid tolerant Saccharomyces cerevisiae for ethanol production from lignocellulosic agricultural waste

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Trichoderma harzianumとLentinula edodesの間の桔抗作用について

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Bioethanol Production from Sugar Cane Syrup by Thermo-tolerant Yeast, Kluyveromyces marxianus DMKU3-1042, using Fed-batch and Repeated-batch Fermentation in a Nonsterile System

Kasetsart Journal. Natural Sciences, 2012

Ethanol production from sugar cane syrup was carried out to compare the effi ciency of batch-, fe... more Ethanol production from sugar cane syrup was carried out to compare the effi ciency of batch-, fed-batch and repeated-batch fermentation using Kluyveromyces marxianus DMKU3-1042. The sugar cane syrup medium contained 18% total sugar, 0.1% (NH4)2SO4, 0.1% KH2PO4, and 0.1% MgSO4.7H2O, with the pH adjusted to 4.5. Experiments were carried out in a 2.5 L jar fermenter at a controlled temperature of 35 °C and an agitation speed of 300 rpm without aeration. All experiments were carried out in a nonsterile system that resembled the process used in the bioethanol fermentation industry in Thailand. The ethanol concentration from batch fermentation was lowest among the three processes where 6.35% w/v was achieved in 72 hr resulting in an ethanol productivity of 0.88 g.L -1 . hr -1 and the ethanol yield corresponded to 82.73% of the theoretical yield. For fed-batch fermentation, the exponential feeding scheme barely improved the ethanol yields while sigmoidal feeding gave a considerably higher...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 3Ep06 Isolation, Selection and Characterization of Thermotolerant Yeasts for Ethanol Production

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Comparison of glucose repression between thermotolerant yeast Kluyveromyces marxianus and Saccharomyces cerevisiae

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Yeast diversity in Huay Kar Kang wildlife sanctuary

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 2Dp03 Yeasts on Agronomic Crop Phylloplane : Description of Novel Species and Potential in Agricultural Application

Bookmarks Related papers MentionsView impact

Research paper thumbnail of An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand

Journal of Fungi, 2021

Ascomycetous yeast species in the genus Wickerhamomyces (Saccharomycetales, Wickerhamomycetaceae)... more Ascomycetous yeast species in the genus Wickerhamomyces (Saccharomycetales, Wickerhamomycetaceae) are isolated from various habitats and distributed throughout the world. Prior to this study, 35 species had been validly published and accepted into this genus. Beneficially, Wickerhamomyces species have been used in a number of biotechnologically applications of environment, food, beverage industries, biofuel, medicine and agriculture. However, in some studies, Wickerhamomyces species have been identified as an opportunistic human pathogen. Through an overview of diversity, taxonomy and recently published literature, we have updated a brief review of Wickerhamomyces. Moreover, two new Wickerhamomyces species were isolated from the soil samples of Assam tea (Camellia sinensis var. assamica) that were collected from plantations in northern Thailand. Herein, we have identified these species as W. lannaensis and W. nanensis. The identification of these species was based on phenotypic (mor...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effects of temperature and relative humidity on Aflatoxin B1 reduction in corn grains and antagonistic activities against Aflatoxin-producing Aspergillus flavus by a volatile organic compound-producing yeast, Kwoniella heveanensis DMKU-CE82

BioControl, 2021

As shown in our previous study, Kwoniella heveanensis DMKU-CE82, a volatile organic compound (VOC... more As shown in our previous study, Kwoniella heveanensis DMKU-CE82, a volatile organic compound (VOC)-producing yeast, demonstrated promising antagonistic activity against aflatoxin-producing strain of Aspergillus flavus . This yeast’s volatile organic compounds (VOCs) could reduce Aflatoxin B1 (AFB1) in corn grains. In the current study, we evaluated the effect of temperatures and relative humidity on AFB1 reduction during grain storage when co-incubated with this VOC-producing yeast. The VOCs produced by K. heveanensis DMKU-CE82 could promote reduction of AFB1 to less than 20 part per billion (ppb) in the fungal contaminated corn grains under most storage conditions at 35 °C. The major VOCs produced by 2-, 4-, and 6-day-old yeast cultures were closely matched to 3-methyl-1-butanol, 2-methyl-1-butanol, hydrazine-1-1-dimethyl, and butanoic acid-3-methyl. In addition, this yeast strain had the ability to produce β-1,3-glucanase, amylase, cellulase, chitinase, siderophores, and biofilms. Scanning electron microscopy also confirmed the antagonistic activity of K. heveanensis DMKU-CE82 as it caused structural damage to conidia and inhibited the development of mycelia and conidiophores in both direct fungal–yeast interaction and the VOC method in corn grains. These results demonstrated that this yeast strain could be a promising biocontrol agent against aflatoxin-producing fungi in agricultural products.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Phylloplane Yeasts in Tropical Climates

Yeasts in Natural Ecosystems: Diversity, 2017

Phylloplane, which refers to the surfaces of aboveground parts of plants, includes mainly leaves ... more Phylloplane, which refers to the surfaces of aboveground parts of plants, includes mainly leaves and has been recognized as an important habitat for microorganisms. The growth of phylloplane microorganisms is dependent on organic and inorganic substances on the leaf surfaces that are either secreted by the plant or originate from external sources. Several factors that structure the microbial communities in phylloplane such as leaf age, plant species, growing conditions, environmental factors, geography and competing microorganisms have been reported. To date, the diversity of microorganisms in the phylloplane has been studied intensively. However, only a small number of articles have focused on yeasts. In this chapter, we review the methodologies, both culture-dependent and culture-independent methods, which have been utilized for assessment of leaf-associated yeasts. The diversity of phylloplane yeasts in the tropical regions assessed by those techniques is discussed. In the course of investigations, phylloplane yeasts in tropical countries have been shown to be highly diverse. Many novel yeast species have been discovered by both culture-dependent and culture-independent methods. These studies suggest that extensive studies of phylloplane yeasts from tropical regions will lead to recovering a so far underappreciated diversity.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae

International Journal of Systematic and Evolutionary Microbiology, 2020

Seven yeast strains, DMKU VGT1-14T, DMKU VGT1-19T, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DM... more Seven yeast strains, DMKU VGT1-14T, DMKU VGT1-19T, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DMKU VGT2-19 and DMKU VGT6-14, were isolated from a grease trap in Thailand and two strains, SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, these strains represented two novel yeast species of the genus Wickerhamiella. In terms of pairwise sequence similarity, four strains, DMKU VGT1-14, DMKU-JMGT1-32, DMKU VGT6-14 and SN-102, were closely related to Wickerhamiella infanticola NRRL Y-17858T but differed by 13 nucleotide substitutions with one gap (2.46 %) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2 %) in the ITS region. The strains DMKU VGT1-19T, DMKU-JMGT1-28, DMKU VGT2-06, DMKU VGT2-19 and SJ-1, differed from the type strain of the most closely re...

Bookmarks Related papers MentionsView impact