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Research paper thumbnail of Molecular mechanisms underlying calcium current modulation by nociceptin

Neuroreport, 2004

Nociceptin is a non-opioid peptide that modulates pain response. One of mechanism underlying its ... more Nociceptin is a non-opioid peptide that modulates pain response. One of mechanism underlying its analgesic action is the inhibition of voltage-dependent calcium current (ICa), similar to that of opioids. We investigated the molecular mechanism by which nociceptin inhibits ICa using sensory neurons and a heterologous expression system. ICa inhibition by nociceptin was voltage-dependent, exhibited the slowing of activation kinetics and prepulse facilitation, and was blocked by N-ethylmaleimide, indicating the involvement of Gi/Go protein. ICa inhibition by nociceptin was primarily mediated through binding to its own receptor, ORL-1, but not through affecting other mu-opioid receptors. Thus, our results strongly demonstrate that heterologous cross-talk between ORL1 and muOR is not involved in the ICa inhibition by nociceptin.

Research paper thumbnail of CHANGES IN THE FATTY ACID COMPOSITION OF THE SEEDS OF THREE OIL-BEARING SPECIES DURING INCREASING SEED MATURITY

Canadian Journal of Plant Science, 1964

Research paper thumbnail of Advances in hydrogeologic testing of mineral exploration boreholes

Research paper thumbnail of Owner: City of Charlottesville

Research paper thumbnail of Molecular mechanisms underlying calcium current modulation by nociceptin

Neuroreport, 2004

Nociceptin is a non-opioid peptide that modulates pain response. One of mechanism underlying its ... more Nociceptin is a non-opioid peptide that modulates pain response. One of mechanism underlying its analgesic action is the inhibition of voltage-dependent calcium current (ICa), similar to that of opioids. We investigated the molecular mechanism by which nociceptin inhibits ICa using sensory neurons and a heterologous expression system. ICa inhibition by nociceptin was voltage-dependent, exhibited the slowing of activation kinetics and prepulse facilitation, and was blocked by N-ethylmaleimide, indicating the involvement of Gi/Go protein. ICa inhibition by nociceptin was primarily mediated through binding to its own receptor, ORL-1, but not through affecting other mu-opioid receptors. Thus, our results strongly demonstrate that heterologous cross-talk between ORL1 and muOR is not involved in the ICa inhibition by nociceptin.

Research paper thumbnail of CHANGES IN THE FATTY ACID COMPOSITION OF THE SEEDS OF THREE OIL-BEARING SPECIES DURING INCREASING SEED MATURITY

Canadian Journal of Plant Science, 1964

Research paper thumbnail of Advances in hydrogeologic testing of mineral exploration boreholes

Research paper thumbnail of Owner: City of Charlottesville

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