Selma Onuma - Academia.edu (original) (raw)

Papers by Selma Onuma

Publicatio UEPG: Ciências Biologicas e da Saude, 2013

The Taiamã Ecological Station is an area of 115,55km 2 in the Pantanal biome and is located in th... more The Taiamã Ecological Station is an area of 115,55km 2 in the Pantanal biome and is located in the municipality of Cáceres, Mato Grosso, Brazil. The station is also situated inside an area of great population density of jaguars. Georeferenced images of Panthera onca were taken in the rivers located around and at the borders of the station, with 27 distinct individuals recorded over several years. These photographic records, along with information obtained from the literature, make it plausible to assume that the station's current area is not sufficient to legally protect the feline population in the region. This study suggests an increase in the size of this conservation unit.

Revista do Instituto de Medicina Tropical de São Paulo, 2015

This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp.... more This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp. and Brucella abortus in two conservation units in the Pantanal of Mato Grosso, Brazil. The presence of antibodies in blood samples of eleven jaguars was investigated using autochthonous antigens isolated in Brazil added to reference antigen collection applied to diagnosis of leptospirosis by Microscopic Agglutination Test (MAT). The Rose Bengal test was applied for B. abortus antibodies. Two (18.2%) jaguars were seroreactive for the Leptospira spp. antigen and the serovar considered as most infective in both animals was a Brazilian isolate of serovar Canicola (L01). All jaguars were seronegative for B. abortus. These data indicate that the inclusion of autochthonous antigens in serological studies can significantly increase the number of reactive animals, as well as modify the epidemiological profile of Leptospira spp. infection.

Brazilian Journal of Veterinary Research and Animal Science, 2014

A investigação da contaminação do solo por enteroparasitos já foi efetuada em várias regiões do B... more A investigação da contaminação do solo por enteroparasitos já foi efetuada em várias regiões do Brasil, principalmente em locais que podem apresentar riscos à saúde humana, como creches e praças públicas. No presente trabalho, foi relatada a contaminação do solo por ovos de Toxocara spp., Ascaris spp., tricostrongilídeos e oocistos de protozoários em uma comunidade rural localizada no Pantanal Mato-Grossense. Os resultados do presente estudo ressaltam o risco a que podem estar expostos os moradores de comunidades em condições precárias de habitação e saneamento com relação à infecção por parasitas.

Revista Brasileira de Parasitologia Veterinária, 2014

Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites th... more Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites that cause reproductive and neurological disorders in a wide range of domestic and wild animals. In the present study, the immunofluorescence antibody test (IFAT) was used to investigate the presence of antibodies against T. gondii, N. caninum and S. neurona in the sera of 11 free-living jaguars (Panthera onca) in two protected areas in the Pantanal region of Mato Grosso state, Brazil. Ten jaguars (90.9%) showed seropositivity for T. gondii, eight (72.7%) for S. neurona, and seven (63.6%) for N. caninum antigens. Our findings reveal exposure of jaguars to these related coccidian parasites and circulation of these pathogens in this wild ecosystem. To the best of our knowledge, this is the first serological detection of N. caninum and S. neurona in free-living jaguars.

Ticks and tick-borne diseases, 2014

A novel Ehrlichia genotype most closely related to E. canis was reported in North American cattle... more A novel Ehrlichia genotype most closely related to E. canis was reported in North American cattle in 2010, and a similar agent was subsequently identified in the hemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks and isolated in 2012. The purpose of this study was to determine whether this or other novel ehrlichial agents naturally infect Brazilian cattle. Using PCR targeting the genus-conserved dsb gene, DNA from this novel ehrlichial agent in Brazilian cattle was detected. Attempts to isolate the organism in vitro were performed using DH82 cells, but morulae and ehrlichial DNA could only be detected for approximately one month. In order to further molecularly characterize the organism, PCR was performed using primers specific for multiple E. canis genes (dsb, rrs, and trp36). Sequence obtained from the conserved rrs and dsb genes demonstrated that the organism was 99-100% identical to the novel Ehrlichia genotypes previously reported in North American cattle (rrs gen...

Publicatio UEPG: Ciências Biologicas e da Saude, 2013

The Taiamã Ecological Station is an area of 115,55km 2 in the Pantanal biome and is located in th... more The Taiamã Ecological Station is an area of 115,55km 2 in the Pantanal biome and is located in the municipality of Cáceres, Mato Grosso, Brazil. The station is also situated inside an area of great population density of jaguars. Georeferenced images of Panthera onca were taken in the rivers located around and at the borders of the station, with 27 distinct individuals recorded over several years. These photographic records, along with information obtained from the literature, make it plausible to assume that the station's current area is not sufficient to legally protect the feline population in the region. This study suggests an increase in the size of this conservation unit.

Revista do Instituto de Medicina Tropical de São Paulo, 2015

This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp.... more This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp. and Brucella abortus in two conservation units in the Pantanal of Mato Grosso, Brazil. The presence of antibodies in blood samples of eleven jaguars was investigated using autochthonous antigens isolated in Brazil added to reference antigen collection applied to diagnosis of leptospirosis by Microscopic Agglutination Test (MAT). The Rose Bengal test was applied for B. abortus antibodies. Two (18.2%) jaguars were seroreactive for the Leptospira spp. antigen and the serovar considered as most infective in both animals was a Brazilian isolate of serovar Canicola (L01). All jaguars were seronegative for B. abortus. These data indicate that the inclusion of autochthonous antigens in serological studies can significantly increase the number of reactive animals, as well as modify the epidemiological profile of Leptospira spp. infection.

Brazilian Journal of Veterinary Research and Animal Science, 2014

A investigação da contaminação do solo por enteroparasitos já foi efetuada em várias regiões do B... more A investigação da contaminação do solo por enteroparasitos já foi efetuada em várias regiões do Brasil, principalmente em locais que podem apresentar riscos à saúde humana, como creches e praças públicas. No presente trabalho, foi relatada a contaminação do solo por ovos de Toxocara spp., Ascaris spp., tricostrongilídeos e oocistos de protozoários em uma comunidade rural localizada no Pantanal Mato-Grossense. Os resultados do presente estudo ressaltam o risco a que podem estar expostos os moradores de comunidades em condições precárias de habitação e saneamento com relação à infecção por parasitas.

Revista Brasileira de Parasitologia Veterinária, 2014

Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites th... more Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites that cause reproductive and neurological disorders in a wide range of domestic and wild animals. In the present study, the immunofluorescence antibody test (IFAT) was used to investigate the presence of antibodies against T. gondii, N. caninum and S. neurona in the sera of 11 free-living jaguars (Panthera onca) in two protected areas in the Pantanal region of Mato Grosso state, Brazil. Ten jaguars (90.9%) showed seropositivity for T. gondii, eight (72.7%) for S. neurona, and seven (63.6%) for N. caninum antigens. Our findings reveal exposure of jaguars to these related coccidian parasites and circulation of these pathogens in this wild ecosystem. To the best of our knowledge, this is the first serological detection of N. caninum and S. neurona in free-living jaguars.

Ticks and tick-borne diseases, 2014

A novel Ehrlichia genotype most closely related to E. canis was reported in North American cattle... more A novel Ehrlichia genotype most closely related to E. canis was reported in North American cattle in 2010, and a similar agent was subsequently identified in the hemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks and isolated in 2012. The purpose of this study was to determine whether this or other novel ehrlichial agents naturally infect Brazilian cattle. Using PCR targeting the genus-conserved dsb gene, DNA from this novel ehrlichial agent in Brazilian cattle was detected. Attempts to isolate the organism in vitro were performed using DH82 cells, but morulae and ehrlichial DNA could only be detected for approximately one month. In order to further molecularly characterize the organism, PCR was performed using primers specific for multiple E. canis genes (dsb, rrs, and trp36). Sequence obtained from the conserved rrs and dsb genes demonstrated that the organism was 99-100% identical to the novel Ehrlichia genotypes previously reported in North American cattle (rrs gen...