Sergey Netesov - Academia.edu (original) (raw)
Papers by Sergey Netesov
Molekuliarnaia genetika, mikrobiologiia i virusologiia, 2023
Archives of Virology, 2017
Intervirology, 1994
Summary The hemagglutination (HA) domains of the Venezuelan equine encephalomyelitis (VEE) and th... more Summary The hemagglutination (HA) domains of the Venezuelan equine encephalomyelitis (VEE) and the eastern equine encephalomyelitis (EEE) viruses providing the interaction of virions and red blood cells were studied with the use of a panel of 17 hemagglutination inhibition (HI) monoclonal antibodies (MAbs). A highly conserved domain (C domain) forming alphavirus-group-reactive MAbs was identified in the E2 protein of the
Archives of virology, Jan 7, 2017
In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. ... more In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. Five new rhabdovirus genera and one new nyamivirus genus were established to harbor 41 of these species, whereas the remaining new species were assigned to already established genera. Furthermore, non-Latinized binomial species names replaced all paramyxovirus and pneumovirus species names, thereby accomplishing application of binomial species names throughout the entire order. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
Bulletin of Experimental Biology and Medicine
A comparison of 5'-UTR structures in Siberian isolates of hepatitis C virus showed that the m... more A comparison of 5'-UTR structures in Siberian isolates of hepatitis C virus showed that the majority of genotyped samples had 1b genotype. A correlation was found between HLA antigens and their stable complexes and clinical manifestations of hepatitis C. We revealed immunogenetic criteria of organism's resistance or susceptibility to hepatitis C virus. The presence of hepatitis C virus RNA in the serum determined by the reverse transcription polymerase chain reaction was accompanied by the appearance of pathomorphological signs characteristic of liver tissue infection, but did not correspond to the severity of fibrosis.
Russian Journal of Genetics: Applied Research, 2014
TORCH INFECTIONS TORCH infections are dangerous for pregnant women and for the fetus. TORCH is an... more TORCH INFECTIONS TORCH infections are dangerous for pregnant women and for the fetus. TORCH is an acronym for toxoplasmosis, other agents, rubella, cytomegalovirus, and herpes simplex.
Biochemistry and molecular biology international, 1995
Nucleotide sequences of the genes 5 and 6 of the Marburg virus, Popp strain, were determined. ORF... more Nucleotide sequences of the genes 5 and 6 of the Marburg virus, Popp strain, were determined. ORFs encoding polypeptides VP30 (281 a.a., MW 32,640) and VP24 (253 a.a., MW 28,621) were found. The putative transcription start and stop signals for viral RNA-dependent RNA polymerase were revealed for both genes. Overlapping of genes 5 and 6 was shown. The deduced amino acid sequences of VP30 and VP24 proteins displayed significant homology with the analogous proteins of another filovirus, the Ebola virus (33% and 37%, respectively). The VP24 appeared to have a hydrophobic amino acid composition; content of hydrophobic amino acids was 40.7%. Model of VP24 location in the virion was suggested.
The objective. To determine various bacterial and viral causative agents of acute enteric infecti... more The objective. To determine various bacterial and viral causative agents of acute enteric infections (AEI) in hospitalized adults and to genotype the obtained isolated of viral pathogens. Patients and methods. Molecular and mictobiological methods were used to examine stool samples from 100 adult patients with AEI hospitalized in 2009 to CCH No 1 of Novosibirsk, for the presence of viral (human rota-, noro-, astro-, entero- and bocaviruses) and bacterial (Salmonella spp., Shigella spp., Escherichia coli, Staphylococcus spp., Klebsiella spp.) agents. Rotaviruses of group A were genotyped by the method of multiplex PCR. For genotyping other enteropathogenic viruses the appropriate variable gene segments were sequenced. Results. The studied microorganisms were found in 57% of patients: in 23% - bacteria, in 24% - viruses, in 10% - bacteria and viruses simultaneously. The most frequently Salmonella spp. (19%) and genotypes GII.4 and GII.3 noroviruses (15%) were detected. In 7%, group А ...
Nucleic Acids Research, 1981
A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method ... more A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method is based upon selective chemical modification of 5'-triphosphate groups by an antigen-containing amine followed by affinity chromatography on an absorbent loaded with antibodies specific to this antigen. A purification factor up to 37 may be achieved.
Infection, Genetics and Evolution, 2012
Human astrovirus is one of the etiological agents of acute gastroenteritis in humans, mostly in y... more Human astrovirus is one of the etiological agents of acute gastroenteritis in humans, mostly in young children and elderly people. Complete genome sequencing of four human astrovirus strains isolated in Novosibirsk, Russia was performed. Analysis of these sequences and the sequences available in GenBank database has detected numerous potential recombination breakpoints. For the first time the rate of human astrovirus evolution was estimated based on the genome fragments without recombination breakpoints; the determined rate is typical of the RNA viruses with high evolutionary rate, amounting to approximately 3.7 Â 10-3 nucleotide substitutions per site per year, and for the synonymous changes, 2.8 Â 10-3 nucleotide substitutions per site per year.
Emerging Infectious Diseases, 2003
Eight fatal cases of tick-borne encephalitis with an unusual hemorrhagic syndrome were identified... more Eight fatal cases of tick-borne encephalitis with an unusual hemorrhagic syndrome were identified in 1999 in the Novosibirsk Region, Russia. To study these strains, we sequenced cDNA fragments of protein E gene from six archival formalin-fixed brain samples. Phylogenetic analysis showed tick-borne encephalitis variants clustered with a Far Eastern subtype (homology 94.7%) but not with the Siberian subtype (82%).
Molecular Genetics Microbiology and Virology (Russian version), 2020
Резюме В декабре 2019 г. в Ухане (Китайская Народная Республика) были обнаружены первые случаи пн... more Резюме В декабре 2019 г. в Ухане (Китайская Народная Республика) были обнаружены первые случаи пневмонии неизвестной этиологии. Позже пневмонию связали с новым коронавирусом, и в феврале 2020 г. Всемирная организация здравоохранения (ВОЗ) дала новой болезни название COVID-19, а Международный Комитет по Таксономии Вирусов (ICTV) вирусу, вызывающему ее, дал название SARS-CoV-2. К 11 марта 2020 г., когда вирус распространился на 114 стран, число диагностированных больных достигло 118 тыс., а число умерших-4 тыс., ВОЗ объявила вспышку заболевания пандемией. К 16 апреля 2020 г. число диагностированных больных превысило 2 млн, а число смертей-134 тыс. В данном обзоре мы суммировали актуальную информацию о возникновении и распространении SARS-CoV-2, его эпидемиологии, диагностике, клиническом течении и лечении COVID-19.
Archives of Virology
Yamasaki (山﨑淳紀) 169 • Hironobu Yanagisawa (栁澤広宣) 170 • Gongyin Ye (叶恭银) 171 • Yong-Zhen Zhang (张永... more Yamasaki (山﨑淳紀) 169 • Hironobu Yanagisawa (栁澤広宣) 170 • Gongyin Ye (叶恭银) 171 • Yong-Zhen Zhang (张永振) 172 • Arnfinn Lodden Økland 173
Additional file 3. Video demonstrating â search-likeâ behavior of the CD90+ MSC with active pseud... more Additional file 3. Video demonstrating â search-likeâ behavior of the CD90+ MSC with active pseudopodial movement and capture of the TAMRA+ cell.
Cancer Cell International, 2019
Background: We have characterized the human cell line arised from the Epstein-Barr virus (EBV) po... more Background: We have characterized the human cell line arised from the Epstein-Barr virus (EBV) positive multiple myeloma aspirate subjected to the long-term cultivation. This cell line has acquired the ability to form free-floating spheres and to produce a xenograft upon transplantation into NOD/SCID mice. Methods: Cells from both in vitro culture and developed xenografts were investigated with a number of analytical approaches, including pathomorphological analysis, FISH analysis, and analysis of the surface antigens and of the VDJ locus rearrangement. Results: The obtained results, as well as the confirmed presence of EBV, testify that both biological systems are derived from B-cells, which, in turn, is a progeny of the EBV-transformed B-cellular clone that supplanted the primordial multiple myeloma cells. Next we assessed whether cells that (i) were constantly present in vitro in the investigated cell line, (ii) were among the sphere-forming cells, and (iii) were capable of internalizing a fluorescent TAMRA-labeled DNA probe (TAMRA+ cells) belonged to one of the three types of undifferentiated bone marrow cells of a multiple myeloma patient: CD34+ hematopoietic stem cells, CD90+ mesenchymal stem cells, and clonotypic multiple myeloma cell. Conclusion: TAMRA+ cells were shown to constitute the fourth independent subpopulation of undifferentiated bone marrow cells of the multiple myeloma patient. We have demonstrated the formation of ectopic contacts between TAMRA+ cells and cells of other types in culture, in particular with CD90+ mesenchymal stem cells, followed by the transfer of some TAMRA+ cell material into the contacted cell.
Scientific and Technical Means of Distinguishing Between Natural and Other Outbreaks of Disease, 2001
ABSTRACT
The Role of Biotechnology in Countering BTW Agents, 2001
ABSTRACT
Doklady Biochemistry and Biophysics, 2006
Molekuliarnaia genetika, mikrobiologiia i virusologiia, 2023
Archives of Virology, 2017
Intervirology, 1994
Summary The hemagglutination (HA) domains of the Venezuelan equine encephalomyelitis (VEE) and th... more Summary The hemagglutination (HA) domains of the Venezuelan equine encephalomyelitis (VEE) and the eastern equine encephalomyelitis (EEE) viruses providing the interaction of virions and red blood cells were studied with the use of a panel of 17 hemagglutination inhibition (HI) monoclonal antibodies (MAbs). A highly conserved domain (C domain) forming alphavirus-group-reactive MAbs was identified in the E2 protein of the
Archives of virology, Jan 7, 2017
In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. ... more In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. Five new rhabdovirus genera and one new nyamivirus genus were established to harbor 41 of these species, whereas the remaining new species were assigned to already established genera. Furthermore, non-Latinized binomial species names replaced all paramyxovirus and pneumovirus species names, thereby accomplishing application of binomial species names throughout the entire order. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
Bulletin of Experimental Biology and Medicine
A comparison of 5'-UTR structures in Siberian isolates of hepatitis C virus showed that the m... more A comparison of 5'-UTR structures in Siberian isolates of hepatitis C virus showed that the majority of genotyped samples had 1b genotype. A correlation was found between HLA antigens and their stable complexes and clinical manifestations of hepatitis C. We revealed immunogenetic criteria of organism's resistance or susceptibility to hepatitis C virus. The presence of hepatitis C virus RNA in the serum determined by the reverse transcription polymerase chain reaction was accompanied by the appearance of pathomorphological signs characteristic of liver tissue infection, but did not correspond to the severity of fibrosis.
Russian Journal of Genetics: Applied Research, 2014
TORCH INFECTIONS TORCH infections are dangerous for pregnant women and for the fetus. TORCH is an... more TORCH INFECTIONS TORCH infections are dangerous for pregnant women and for the fetus. TORCH is an acronym for toxoplasmosis, other agents, rubella, cytomegalovirus, and herpes simplex.
Biochemistry and molecular biology international, 1995
Nucleotide sequences of the genes 5 and 6 of the Marburg virus, Popp strain, were determined. ORF... more Nucleotide sequences of the genes 5 and 6 of the Marburg virus, Popp strain, were determined. ORFs encoding polypeptides VP30 (281 a.a., MW 32,640) and VP24 (253 a.a., MW 28,621) were found. The putative transcription start and stop signals for viral RNA-dependent RNA polymerase were revealed for both genes. Overlapping of genes 5 and 6 was shown. The deduced amino acid sequences of VP30 and VP24 proteins displayed significant homology with the analogous proteins of another filovirus, the Ebola virus (33% and 37%, respectively). The VP24 appeared to have a hydrophobic amino acid composition; content of hydrophobic amino acids was 40.7%. Model of VP24 location in the virion was suggested.
The objective. To determine various bacterial and viral causative agents of acute enteric infecti... more The objective. To determine various bacterial and viral causative agents of acute enteric infections (AEI) in hospitalized adults and to genotype the obtained isolated of viral pathogens. Patients and methods. Molecular and mictobiological methods were used to examine stool samples from 100 adult patients with AEI hospitalized in 2009 to CCH No 1 of Novosibirsk, for the presence of viral (human rota-, noro-, astro-, entero- and bocaviruses) and bacterial (Salmonella spp., Shigella spp., Escherichia coli, Staphylococcus spp., Klebsiella spp.) agents. Rotaviruses of group A were genotyped by the method of multiplex PCR. For genotyping other enteropathogenic viruses the appropriate variable gene segments were sequenced. Results. The studied microorganisms were found in 57% of patients: in 23% - bacteria, in 24% - viruses, in 10% - bacteria and viruses simultaneously. The most frequently Salmonella spp. (19%) and genotypes GII.4 and GII.3 noroviruses (15%) were detected. In 7%, group А ...
Nucleic Acids Research, 1981
A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method ... more A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method is based upon selective chemical modification of 5'-triphosphate groups by an antigen-containing amine followed by affinity chromatography on an absorbent loaded with antibodies specific to this antigen. A purification factor up to 37 may be achieved.
Infection, Genetics and Evolution, 2012
Human astrovirus is one of the etiological agents of acute gastroenteritis in humans, mostly in y... more Human astrovirus is one of the etiological agents of acute gastroenteritis in humans, mostly in young children and elderly people. Complete genome sequencing of four human astrovirus strains isolated in Novosibirsk, Russia was performed. Analysis of these sequences and the sequences available in GenBank database has detected numerous potential recombination breakpoints. For the first time the rate of human astrovirus evolution was estimated based on the genome fragments without recombination breakpoints; the determined rate is typical of the RNA viruses with high evolutionary rate, amounting to approximately 3.7 Â 10-3 nucleotide substitutions per site per year, and for the synonymous changes, 2.8 Â 10-3 nucleotide substitutions per site per year.
Emerging Infectious Diseases, 2003
Eight fatal cases of tick-borne encephalitis with an unusual hemorrhagic syndrome were identified... more Eight fatal cases of tick-borne encephalitis with an unusual hemorrhagic syndrome were identified in 1999 in the Novosibirsk Region, Russia. To study these strains, we sequenced cDNA fragments of protein E gene from six archival formalin-fixed brain samples. Phylogenetic analysis showed tick-borne encephalitis variants clustered with a Far Eastern subtype (homology 94.7%) but not with the Siberian subtype (82%).
Molecular Genetics Microbiology and Virology (Russian version), 2020
Резюме В декабре 2019 г. в Ухане (Китайская Народная Республика) были обнаружены первые случаи пн... more Резюме В декабре 2019 г. в Ухане (Китайская Народная Республика) были обнаружены первые случаи пневмонии неизвестной этиологии. Позже пневмонию связали с новым коронавирусом, и в феврале 2020 г. Всемирная организация здравоохранения (ВОЗ) дала новой болезни название COVID-19, а Международный Комитет по Таксономии Вирусов (ICTV) вирусу, вызывающему ее, дал название SARS-CoV-2. К 11 марта 2020 г., когда вирус распространился на 114 стран, число диагностированных больных достигло 118 тыс., а число умерших-4 тыс., ВОЗ объявила вспышку заболевания пандемией. К 16 апреля 2020 г. число диагностированных больных превысило 2 млн, а число смертей-134 тыс. В данном обзоре мы суммировали актуальную информацию о возникновении и распространении SARS-CoV-2, его эпидемиологии, диагностике, клиническом течении и лечении COVID-19.
Archives of Virology
Yamasaki (山﨑淳紀) 169 • Hironobu Yanagisawa (栁澤広宣) 170 • Gongyin Ye (叶恭银) 171 • Yong-Zhen Zhang (张永... more Yamasaki (山﨑淳紀) 169 • Hironobu Yanagisawa (栁澤広宣) 170 • Gongyin Ye (叶恭银) 171 • Yong-Zhen Zhang (张永振) 172 • Arnfinn Lodden Økland 173
Additional file 3. Video demonstrating â search-likeâ behavior of the CD90+ MSC with active pseud... more Additional file 3. Video demonstrating â search-likeâ behavior of the CD90+ MSC with active pseudopodial movement and capture of the TAMRA+ cell.
Cancer Cell International, 2019
Background: We have characterized the human cell line arised from the Epstein-Barr virus (EBV) po... more Background: We have characterized the human cell line arised from the Epstein-Barr virus (EBV) positive multiple myeloma aspirate subjected to the long-term cultivation. This cell line has acquired the ability to form free-floating spheres and to produce a xenograft upon transplantation into NOD/SCID mice. Methods: Cells from both in vitro culture and developed xenografts were investigated with a number of analytical approaches, including pathomorphological analysis, FISH analysis, and analysis of the surface antigens and of the VDJ locus rearrangement. Results: The obtained results, as well as the confirmed presence of EBV, testify that both biological systems are derived from B-cells, which, in turn, is a progeny of the EBV-transformed B-cellular clone that supplanted the primordial multiple myeloma cells. Next we assessed whether cells that (i) were constantly present in vitro in the investigated cell line, (ii) were among the sphere-forming cells, and (iii) were capable of internalizing a fluorescent TAMRA-labeled DNA probe (TAMRA+ cells) belonged to one of the three types of undifferentiated bone marrow cells of a multiple myeloma patient: CD34+ hematopoietic stem cells, CD90+ mesenchymal stem cells, and clonotypic multiple myeloma cell. Conclusion: TAMRA+ cells were shown to constitute the fourth independent subpopulation of undifferentiated bone marrow cells of the multiple myeloma patient. We have demonstrated the formation of ectopic contacts between TAMRA+ cells and cells of other types in culture, in particular with CD90+ mesenchymal stem cells, followed by the transfer of some TAMRA+ cell material into the contacted cell.
Scientific and Technical Means of Distinguishing Between Natural and Other Outbreaks of Disease, 2001
ABSTRACT
The Role of Biotechnology in Countering BTW Agents, 2001
ABSTRACT
Doklady Biochemistry and Biophysics, 2006