Maribel Serrano - Academia.edu (original) (raw)
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Papers by Maribel Serrano
European Journal of Radiology Extra, 2010
Sequestration spectrum of the lung Pulmonary sequestration Intralobar sequestration Systemic arte... more Sequestration spectrum of the lung Pulmonary sequestration Intralobar sequestration Systemic arterial supply to the lung Congenital pulmonary disease a b s t r a c t Intralobar pulmonary sequestration and anomalous systemic arterial supply to the normal lung are two anomalies pertaining to the pulmonary sequestration spectrum. This term brings together a group of related congenital anomalies with a common embryogenesis. Most cases of pulmonary sequestration are unilateral. Bilateral presentation is very rare, with few cases reported. We present two patients with intralobar pulmonary sequestration in the left lung and systemic arterial supply to the otherwise normal right lung. This association is extremely unusual and, to the best of our knowledge, this is the first time it has been diagnosed with multidetector computerized tomography.
Hepatology, 2002
Liver cells from p21 Cip1؊/؊ mice subjected to partial hepatectomy (PH) progress into DNA synthes... more Liver cells from p21 Cip1؊/؊ mice subjected to partial hepatectomy (PH) progress into DNA synthesis faster than those from wild-type mice. These cells also show a premature induction of cyclin E/cyclin-dependent kinase (CDK) 2 activity. We studied the mechanisms whereby cells lacking p21 Cip1 showed a premature induction of this activity. Whereas the levels of CDK2, cyclin E, and p27 Kip1 were similar in both wild-type and p21 Cip1؊/؊ mice, those of the activator CDC25A were much higher in p21 Cip1؊/؊ quiescent and regenerating livers than in wild-type animals. Moreover, p21 Cip1؊/؊ cells also showed a premature translocation of CDC25A from cytoplasm into the nucleus. The ectopic expression of p21 Cip1 into mice embryo fibroblasts from p21 Cip1؊/؊ mice decreased the levels of CDC25A and delayed its nuclear translocation. The levels of CDC25A messenger RNA in p21 Cip1؊/؊ cells were higher than in wild-type cells, suggesting that this increase might be responsible, at least in part, for the high levels of CDC25A protein in these cells. Thus, the results reported here indicate that p21 Cip1 regulates the levels and the intracellular localization of CDC25A. We also found a good correlation between CDC25A nuclear translocation and cyclin E/CDK2 activation. In conclusion, premature translocation of CDC25A to the nucleus might be involved in the advanced induction of cyclin E/CDK2 activity and DNA replication in cells from animals lacking p21 Cip1 . (HEPATOLOGY 2002;35:1063-1071.) Abbreviations: CDK, cyclin-dependent kinase; PCNA, proliferating cell nuclear antigen; PH, partial hepatectomy; MEF, mice embryonary fibroblast; FCS, fetal calf serum. From the
European Journal of Radiology Extra, 2010
Sequestration spectrum of the lung Pulmonary sequestration Intralobar sequestration Systemic arte... more Sequestration spectrum of the lung Pulmonary sequestration Intralobar sequestration Systemic arterial supply to the lung Congenital pulmonary disease a b s t r a c t Intralobar pulmonary sequestration and anomalous systemic arterial supply to the normal lung are two anomalies pertaining to the pulmonary sequestration spectrum. This term brings together a group of related congenital anomalies with a common embryogenesis. Most cases of pulmonary sequestration are unilateral. Bilateral presentation is very rare, with few cases reported. We present two patients with intralobar pulmonary sequestration in the left lung and systemic arterial supply to the otherwise normal right lung. This association is extremely unusual and, to the best of our knowledge, this is the first time it has been diagnosed with multidetector computerized tomography.
Hepatology, 2002
Liver cells from p21 Cip1؊/؊ mice subjected to partial hepatectomy (PH) progress into DNA synthes... more Liver cells from p21 Cip1؊/؊ mice subjected to partial hepatectomy (PH) progress into DNA synthesis faster than those from wild-type mice. These cells also show a premature induction of cyclin E/cyclin-dependent kinase (CDK) 2 activity. We studied the mechanisms whereby cells lacking p21 Cip1 showed a premature induction of this activity. Whereas the levels of CDK2, cyclin E, and p27 Kip1 were similar in both wild-type and p21 Cip1؊/؊ mice, those of the activator CDC25A were much higher in p21 Cip1؊/؊ quiescent and regenerating livers than in wild-type animals. Moreover, p21 Cip1؊/؊ cells also showed a premature translocation of CDC25A from cytoplasm into the nucleus. The ectopic expression of p21 Cip1 into mice embryo fibroblasts from p21 Cip1؊/؊ mice decreased the levels of CDC25A and delayed its nuclear translocation. The levels of CDC25A messenger RNA in p21 Cip1؊/؊ cells were higher than in wild-type cells, suggesting that this increase might be responsible, at least in part, for the high levels of CDC25A protein in these cells. Thus, the results reported here indicate that p21 Cip1 regulates the levels and the intracellular localization of CDC25A. We also found a good correlation between CDC25A nuclear translocation and cyclin E/CDK2 activation. In conclusion, premature translocation of CDC25A to the nucleus might be involved in the advanced induction of cyclin E/CDK2 activity and DNA replication in cells from animals lacking p21 Cip1 . (HEPATOLOGY 2002;35:1063-1071.) Abbreviations: CDK, cyclin-dependent kinase; PCNA, proliferating cell nuclear antigen; PH, partial hepatectomy; MEF, mice embryonary fibroblast; FCS, fetal calf serum. From the