Seyed Jalal Hosseinimehr - Academia.edu (original) (raw)

Papers by Seyed Jalal Hosseinimehr

Journal of Medicinal Plants, 2020

Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant t... more Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant that has more than 160 species in the world. In addition, this plant is an important medicinal plant in traditional and modern medicines. Objective: The aim of this study was to evaluate the phytochemical profiles and physicochemical properties, antioxidant and anti-inflammatory activities of Colchicum kurdicum (Bornm.) Stef. corm. Methods: Phytochemical profiles includedtotal tropolone alkaloid, total phenolic/total tannin and total flavonoid contents were determined by spectrophotometric method. Moreover, tropolone alkaloid profiles was analyzed by HPLC method. Physicochemical properties including macroscopic and organoleptic properties, solubility, foreign matter, ash values and heavy metals were evaluated based on pharmacopeial protocoles. In addition, in vitro anti-inflammatory and antioxidant activities of the plant were determined. Results: Total tropolone alkaloids, phenol, tannin and flavonoid contents of the corm were estimated to be 0.652, 0.426, 0.052 and 0.325 g/100 g corm. In HPLC analysis, N-deacetyl-N-formyl colchicine, colchifoline, colchicoside and cornigerine were calculated as highest bioactive tropolone alkaloids. Physicochemical properties were determined within an acceptable range for the pharmacopoeia. Moreover, the anti-inflammatory and antioxidant activities in 10 mg/ml of methanolic extract were estimated 84.7 % and 94.8 %, respectively. Conclusion: The corm of the Colchicum kurdicum had the main bioactive compounds. In addition, these compounds have the valuable biological activities as anti-inflammatory and antioxidant activities.

Cellular and molecular biology, 2016

Losartan is an angiotensin II receptor (AT-II-R) blocker that is widely used by human for blood p... more Losartan is an angiotensin II receptor (AT-II-R) blocker that is widely used by human for blood pressure regulation. Also, it has antitumor property. In this study, we investigated the radiosensitizing effect of losartan on cellular toxicity induced by ionizing radiation on prostate cancer and non-malignant fibroblast cells. Human prostate cancer (DU-145) and human non-malignant fibroblast cells (HFFF2) were treated with losartan at different concentrations (0.5, 1, 10, 50 and 100 µM) and then these cells were exposed to ionizing radiation. The cell proliferation was determined using MTT assay. Our results showed that losartan exhibited antitumor effect on prostate cancer cells; it was reduced cell survival to 66% at concentration 1 µM. Losartan showed an additive killing effect in combination with ionizing radiation on prostate cancer cell. The cell proliferation was reduced to 54% in the prostate cancer cells treated with losartan at concentration 1 µM in combination with ionizing...

Avicenna Journal of Phytomedicine, 2015

Objectives: Burn injury is one of the most health-threatening problems in the world. Malva sylves... more Objectives: Burn injury is one of the most health-threatening problems in the world. Malva sylvestris (M. sylvestris) flowers have a high mucilage content and are used as a remedy for cut wound and dermal infected wounds in Iranian folklore Medicine. The purpose of this study was to investigate the effect of M. sylvestris cream on the second degree burn injury in rats. Materials and Methods: Five groups of 10 rats per group were burned with hot metal plate. Animals were administrated divided as control, normal saline, standard silver sulfadiazine 1% (SSD), 5% M. sylvestris, and 10% M. sylvestris into separate groups. Wound area, percentage of wound contraction, and histological and bacteriological assessments were evaluated. Results: Wound sizes were not significantly different among groups on 1st and 3rd days after burn injury, while they were significantly different among groups after 7th day post-burn injury. The average areas of wounds on the 15th day were 7.5±2.9, 6.7±2, 10.5±1...

Journal of Bioenergetics and Biomembranes, 2019

International Journal of Reproductive BioMedicine, 2018

Background: Cyclophosphamide (CP), as an anticancer agent, causes ovarian toxicity and subsequent... more Background: Cyclophosphamide (CP), as an anticancer agent, causes ovarian toxicity and subsequent infertility in women. Atorvastatin (ATV) at a low dose has antioxidant and antiinflammatory properties. Objective: The aim of this study was to investigate the protective effect of ATV against CPinduced ovarian injury in rat. Materials and Methods: In this experimental study, thirty-two female Wistar rats were randomly divided into four groups as I) control, II) ATV (10 mg/kg), III) CP (150 mg/kg), and IV) CP +ATV. The ATV treated groups were received ATV for 10 days via oral gavage. In the CP+ATV group, ATV was administrated on 5 days before and 5 days after CP injection. Histological structure, apoptosis (caspase-3), oxidative stress parameters as malondialdehyde, reactive oxygen species, protein carbonyl levels and cell viability were evaluated in ovary tissue by histological scores, immunohistochemistry, histochemical and biochemical assays. The levels of estrogen and progesterone hormones were measured on the 12th day of study. Results: ATV pretreatment significantly decreased the levels of oxidative stress biomarkers as malondialdehyde, reactive oxygen species and protein carbonyl levels and increased cell death in CP-treated rats as compared with the CP alone group. ATV significantly increased estrogen and progesterone levels in CP-treated rats. In addition, the histological examination showed ATV mitigated acute inflammation, degenerative cells in stroma and follicles, stromal edema, vacuolization, atresia of the follicles and congestion of blood vessels in the CP-treated animals. Furthermore, ATV significantly reduced immunoreactivity level of caspase-3 in CPtreated rats. Conclusion: Our results showed that the ATV with antioxidant and anti-apoptosis (caspase-3) activities protected ovarian against CP-induced toxicity.

Nuclear medicine review. Central & Eastern Europe, Jan 10, 2018

In this study, we investigated the protective effect of vitamin E against methotrexate (MTX)-indu... more In this study, we investigated the protective effect of vitamin E against methotrexate (MTX)-induced hepatotoxicity by quantitative liver 99mTc-phytate uptake and liver imaging and to compare its effect with histopathology in rat. Rats were divided into five groups as control, solvent, Vit E (100 mg/kg), MTX (20 mg/kg), Vit E + MTX and. Vit E was intraperitoneally administrated for 17 days before MTX injection and continued for 4 days. 99mTc-phytate was injected through the tail of rats after the drug administration. The percentage of the injected dose per gram of liver and spleen tissues (%ID/g) was calculated. Liver imaging was obtained with gamma camera. In other experiment, liver of treated rats were assessed for histopathology. 99mTc-phytate uptake per gram tissue of the livers as %ID/g in control, solvent, MTX, Vit E, Vit E + MTX and MTX groups were 8.99%  1.37, 8.53%  2.91, 8.65%  3.84, 3.22%  1.09 and 8.38%  2.68. Vit E administration with MTX resulted in a significant ...

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Iranian journal of basic medical sciences, 2018

The monitoring of cancer treatment response to chemotherapy is considered an essential strategy f... more The monitoring of cancer treatment response to chemotherapy is considered an essential strategy for follow-up of patients. The aim of this study was to evaluate the use ofTc-glucarate as a radiotracer for in vivo quantification and visualization of necrotic area and therapeutic effect of paclitaxel in ovarian cancer xenografted nude mice. After implantation of human ovarian cancer (SKOV-3) in nude mice, tumor xenografted mice were enrolled in two groups as control and treatment (paclitaxel) groups.Tc-glucarate uptakes were quantified in tumors of control and treatment groups and also tumor imaging was performed with a gamma camera. The necrotic and viable areas of tumor and tumoral masses were evaluated through histopathological and macroscopic observations, respectively. Tc-glucarate uptake in tumor of treatment group was higher than control group.Tc-glucarate uptake in ovarian tumor was clearly visualized with gamma imaging in both groups, but paclitaxel treated group showed highe...

Advances in Pharmacological Sciences, 2017

Background. We compared the efficacy ofP. granatum(P) flower extract with that of silver sulfadia... more Background. We compared the efficacy ofP. granatum(P) flower extract with that of silver sulfadiazine (SSD) for treating thermal burn injuries in rats.Methods. Ten Wistar rats in each group were topically given base cream, normal saline, cream containing 1% SSD, or creams containing 5% or 10%Punica granatumflower extract. The treatments were administered once daily until complete wound healing was observed. The wound area and healing time were assessed. In addition, percentage wound contraction and histopathological characteristics such as neovascularization and collagen formation were determined. The tannin content inP. granatumextract was determined.Results. The decrease in the average size of wounds on day 15 of the treatment was higher in rats treated with creams containingP. granatumextract than in rats treated with cream containing SSD (2.8±0.9 cm2versus8.4±3.2 cm2). The wounds completely healed on day 25 of the treatment in rats treated with creams containingP. granatumflower...

Pharmaceutical and Biomedical Research, 2015

Bleomycin (BLM) as an anti-cancer agent causes tissue toxicities through DNA damaging and cell de... more Bleomycin (BLM) as an anti-cancer agent causes tissue toxicities through DNA damaging and cell deaths. The aim of this study was to investigate the effects of thymol against genotoxicity and anti-proliferation induced by BLM in normal human lymphocytes and ovarian cancer cells. Peripheral blood samples were collected from human volunteers and were incubated with thymol at different concentrations at 50, 100, and 150 μM. After 2 h incubation, the whole blood was treated with BLM. Then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis blocked binucleated lymphocyte. Human ovarian cancer cells (SKOV-3) were treated with thymol at various concentrations and/or BLM with their combinations and then cell viability were evaluated. Incubation of whole blood with thymol exhibited a significant decrease in the incidence of micronuclei in lymphocytes caused by BLM, as compared with similarly BLM-treated lymphocytes without thymol. Neither enhanced cell death nor cell protective effect was observed using thymol pre-treatment of SKOV-3 cells. This study showed that thymol selectively protects human lymphocytes against DNA damage induced by BLM without any protection on cancer cell. This result is promising for using this natural product in treatment of ovarian cancer with BLM.

Asian Pacific Journal of Cancer Prevention, 2015

Background: Although radiotherapy is one of the most effective strategies in the treatment of can... more Background: Although radiotherapy is one of the most effective strategies in the treatment of cancers, it is associated with short and long term side effects on normal tissues. Zataria multiflora Boiss (Laminacea) (ZM) has several biological properties such as antioxidant and anti-inflammation activities.Here we investigated cell killing effects of a hydroalcoholic Zataria multiflora extract on cell death induced by ionizing radiation in a human glioblastoma cell line (A172) and human non-malignant fibroblasts (HFFF2) in vitro. Materials and Methods: A172 and HFFF2 cells were treated with a hydroalcoholic extract of dried aerial parts of Zataria multiflora at different concentrations (25, 50, 100, 150 and 200 μg/ml) and then exposed to ionizing radiation (IR). Cell proliferation and DNA fragmentation were evaluated. Thymol content in the extract was analyzed and quantified by HPLC methods. Results: A172 cell proliferation was significantly inhibited by ZM. The percentage cell survival was 91.8±8.57 for cells treated with 200 μg/ml of ZM extract alone while it was 76.0±4.27 and 66.2±8.42 for cells treated with ZM and exposed to IR at doses of 3Gy and 6Gy, respectively. Radiation-induced apoptosis in A172 cells was significantly increased following treatment with ZM at doses of 200 μg/ml. ZM extract did not exhibit any enhanced cell killing effects and apoptosis caused by IR on HFFF2 cells. Conclusions: These data show selective radiosensitization effects of ZM in A172 cells apparently due to increased radiation-induced apoptosis.

Asian Pacific Journal of Cancer Prevention, 2012

Background: Bladder cancer is one the most common malignancies of the genitourinary tract. The pr... more Background: Bladder cancer is one the most common malignancies of the genitourinary tract. The present study aimed to assess the epidemiology, of bladder cancer in Mazandaran, a large province in northern Iran as high-incidence cancer area, during a 2-year period. Methods: The data for this study were obtained from the population-based cancer registry of the Vice-Chancellory for Health Affairs of Mazandaran University of Medical Sciences and Mazandaran hospitals between March 1, 2010 and March 1, 2011. Demographic data, including sex, age, residency and symptoms were investigated through careful review of medical records. Using a questionnaire protocol, several variables were assessed for these cases such as smoking, history of opium, vegetable consumption habits, and history of other cancers. Results: A total of 112 cases were analyzed, 98 (87.5%) in men and 14 (12.5%) in women (mean age of 68.0±14.6 years). Urban and rural residence were 60.7% and 39.3%. Tobacco and opium use were found in 45.5% and 21.4% of patients, respectively. Approximately 60% consumed vegetables an average of fewer than one time per day. Hematuria was the first symptom in these cases which were mainly diagnosed as having bladder cancer by ultrasonography. Conclusion: The results showed that bladder cancer tends to be found in the elderly and the male to female ratio is high. Macroscopic hematuria is a very important symptom for indicating probably urothelial tumor that should be followed up patients with transabdominal ultrasonography as a routine modality.

World journal of plastic surgery, 2015

Burn is still a majordevastating condition in emergency medicine departments among both genders a... more Burn is still a majordevastating condition in emergency medicine departments among both genders and all age groups in all developed and developing countries, leading to physical, psychological scars and economical burden. The present study aimed to determine the healing effect of topical treatment with Arnebia euchroma on second-degree burn wound in rats. Fifty rats were divided into 4 equal groups receiving the ointment base, normal saline (NS), standard 1% silver sulfadiazine (SSD), and 5% and 10% Arnebia euchroma ointments (AEO). The mean of burn area, percentage of wound contraction, histopathological and bacteriological assessments in the injured area were dtermined during the study. Average area of wound on the 10(th) day was 10.2±2.3, 8.4±2.6, 12.4±2.5, 5.9±2.2 and 5.7±2 cm(2) for ointment base, NS, 1% SSD, and 5% and 10% AEO, respectively. Wound size was significantly lower in 10% AEO than 1% SSD and control groups on the 10(th) day post-burn injury. On day 11, the percentag...

Radiation Oncology Journal, 2015

Purpose: Mefenamic acid (MEF) as a non-steroidal anti-inflammatory drug is used as a medication f... more Purpose: Mefenamic acid (MEF) as a non-steroidal anti-inflammatory drug is used as a medication for relieving of pain and inflammation. Radiation-induced inflammation process is involved in DNA damage and cell death. In this study, the radioprotective effect of MEF was investigated against genotoxicity induced by ionizing radiation in human blood lymphocytes. Materials and Methods: Peripheral blood samples were collected from human volunteers and incubated with MEF at different concentrations (5, 10, 50, or 100 μM) for two hours. The whole blood was exposed to ionizing radiation at a dose 1.5 Gy. Lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis blocked binucleated lymphocyte. Results: A significant decreasing in the frequency of micronuclei was observed in human lymphocytes irradiated with MEF as compared to irradiated lymphocytes without MEF. The maximum decreasing in frequency of micronuclei was observed at 100 μM of MEF (38% decrease), providing maximal protection against ionizing radiation. Conclusion: The radioprotective effect of MEF is probably related to anti-inflammatory property of MEF on human lymphocytes.

Biomarkers in medicine, 2014

This study aimed to assess the urinary concentrations of epidermal growth factor (EGF) and kidney... more This study aimed to assess the urinary concentrations of epidermal growth factor (EGF) and kidney injury molecule 1 (KIM-1) in patients with hydronephrosis. Neonates with a history of prenatal hydronephrosis were enrolled in three groups. Group 1 included neonates with severe obstruction; group 2 included neonates with milder obstruction; and group 3 included neonates with normal findings on postnatal ultrasonography. 59 neonates were enrolled. The EGF: Cr and KIM-1: Cr ratios were significantly higher in group 1 than in group 2 (p = 0.016 and 0.015, respectively). The cut-off values were measured as 16.855 (sensitivity 71%, specificity 77%) and 0.4765 (sensitivity 81%, specificity 71%) for EGF:Cr and KIM-1:Cr ratios, respectively. The values were significantly higher in group 1 than in group 2. Evaluation of the urinary KIM-1:Cr ratio may help identify neonates with severe obstructive hydronephrosis.

Burn is an important life-threatening disturbance for human. The purpose of this study was review... more Burn is an important life-threatening disturbance for human. The purpose of this study was reviewing natural products recommended by resources of Iranian traditional medicine (ITM) for treatment of burn. We investigated the books of philosophers of Iranian traditional medicine; written sources from the 9 th century AD with the words of "Hergh" and "Fire burn" were studied. Bibliography of sources, name of products (plant, animal or mineral), using methods, and the nature of the products were recorded. More details of these natural products were evaluated in recent databases with focusing on burn treatment. 89 natural products to treat burns were presented in 11 traditional written sources. 46.1% of the products were in more than one source. 76.4% of the products were of herbal origin, 15.7% of mineral origin, and 7.9% were of animal origin. Most of the presented products had cold and dry nature. 18% of Materia have been described to have amazing effect or to be extremely beneficial. Some of reported products in the study are approved by the new standard and scientific methods; but most of the cases are not. This is a suitable issue for researchers that would like work on Iranian traditional medicine.

Acute pyelonephritis (APN) is the main predisposing factor for renal cortical scar (RCS) performi... more Acute pyelonephritis (APN) is the main predisposing factor for renal cortical scar (RCS) performing. Hypertension is a complication of RCS and appears in 10-20 Percent of patient in their life. Diagnosis and treatment of APN are necessary for prevention of RCS. The aim of this review is assessment of 99mTc-DMSA (99mTc-dimercaptosuccinic acid) scan value in diagnosis of APN and RCS and comparison of its results with other diagnostic methods such as radiology and ultrasonography. Ultrasonography (US) showed almost 39% of APN that diagnosed with 99mTC-DMSA scan. Computed Tomography Scan (CT scan) is a sensitive and effective method in diagnosis of APN and its complication. Spiral CT scan and Magnetic Resonance Imaging (MRI) are sensitive and relatively reliable tools in diagnosis of APN; however they are not practicable in the first evaluation and fallow up of infant and children with acute urinary tract infection. Power Doppler Ultrasonography (PDUS) demonstrated a sensitivity of 70 percent and a specificity of 85 percent in diagnosis of APN. 99m Tc-DMSA scan is the most sensitive diagnostic tool of APN and RCS with a sensitivity and specificity of 89-91 and 100 percent, respectively. Because 99m Tc-MAG3(99mTc-mercapto-acetylglycyl-glycyl-glycine) provides adequate images for assessment of renal cortex and accurate measurement of deferential renal function (DRF) as compared to 99m Tc DMSA, and it provides important information about urodynamics of both kidneys. Authors considered that it is the best time to replace the 99m Tc-DMSA with 99m Tc-MAG3 in most nephrourologic disorders in pediatric patients to avoids unnecessary radiation to the children, however, it need more studies. Some researchers recommended that follow-up 99m Tc-DMSA scan should be perform after 12 month of APN, since in comparison with at 6 month follow-up 99m Tc-DMSA scan, study at 12month after APN can demonstrates more permanent lesion.

Surgery Today, 2009

Burn injury is associated with a high incidence of death and disability; yet its management remai... more Burn injury is associated with a high incidence of death and disability; yet its management remains problematic and costly. We conducted this clinical study to evaluate the efficacy of aloe vera cream for partial thickness burn wounds and compare its results with those of silver sulfadiazine (SSD). Thirty patients with similar types of second-degree burns at two sites on different parts of the body were included in this study. Each patient had one burn treated with topical SSD and one treated with aloe cream, randomly. The rate of re-epithelialization and healing of the partial thickness burns was significantly faster in the site treated with aloe than in the site treated with SSD (15.9 +/- 2 vs 18.73 +/- 2.65 days, respectively; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). The sites treated with aloe were completely healed in less than 16 days vs 19 days for the sites treated with SSD. These results clearly demonstrated the greater efficacy of aloe cream over SSD cream for treating second-degree burns.

Molecular and Cellular Biochemistry, 2006

Oxymetholone is a 17alpha -alkylated anabolic-androgenic steroid. This drug can stimulate bone ma... more Oxymetholone is a 17alpha -alkylated anabolic-androgenic steroid. This drug can stimulate bone marrow cells and increase the blood cells in the peripheral blood vessels. It has been used for the treatment of anemia caused by low red cell production. Since oxymetholone has hematopoietic effect, we studied radioprotective effects of this drug in mice. In this study, we determined percentage of survival, dose-reduction factor (DRF) and hematological parameters in irradiated mice which treated with or without oxymetholone. Oxymetholone administrated at different doses 80, 160, 320, 640 mg/kg by gavages at 24 h before 8 Gy gamma irradiation. At 30 days after treatment, the following percentage of animals survival in each group was as: 80 mg/kg, 50%; 160 mg/kg, 50%; 320 mg/kg, 55%; 640 mg/kg, 75% and vehicle, 15%. Percentage of survival increased in all of treated groups statistically compared with irradiated-vehicle group. In the groups treated by oxymetholone, maximum protection was realized at 640 mg/kg. In order to calculate the DRF for oxymetholone, mice were exposed to whole-body gamma irradiation with dose ranges between 5.83 and 11.23 Gy. The probit line for oxymetholone-treated mice was shifted to the right with a DRF of 1.14. In mice exposed to whole-body gamma-irradiation (4 Gy), an oral administration of 640 mg/kg oxymetholone ameliorated radiation-induced decreases in circulating platelets and erythrocytes, but had a less effect on total number of WBC. These results demonstrate that oxymetholone stimulates myelopoiesis and thrombocytopenia and enhances survival in mice after ionizing radiation.

Journal of Pharmacy and Pharmacology, 2005

The protective effect of citrus extract was investigated by using the micronucleus assay for anti... more The protective effect of citrus extract was investigated by using the micronucleus assay for anticlastogenic activity in mouse bone marrow cells; liver glutathione (GSH) content was determined against toxicity induced by cyclophosphamide. Mice were orally (gavage) pretreated with solutions of citrus peel extract (Citrus aurantium var. amara) prepared at three different doses (100, 200 and 400 mg kg−1 body weight) for 7 consecutive days. Then mice were injected intraperitoneally on the seventh day with cyclophosphamide (50 mg kg−1) and after 24 h killed for the evaluation of micronucleated polychromatic erythrocytes (MnPCEs) in bone marrow cells. Non-protein thiol levels in liver were estimated in mice injected with citrus extract with or without cyclophosphamide treatment. Administration of citrus extract before cyclophosphamide treatment significantly reduced the frequency of MnPCEs in mice bone marrow compared with the group treated with cyclophosphamide alone (P<0.0001–0.05). ...

The Journal of Alternative and Complementary Medicine, 2010

Objective: Aloe vera is an herbal medicine, which has wound healing effects in burn injury. This ... more Objective: Aloe vera is an herbal medicine, which has wound healing effects in burn injury. This study assessed the effects of Aloe vera cream in reducing postoperative pain, postdefection pain, and its promotion of wound healing after open hemorrhoidectomy. Design: A prospective, randomized, double-blind, placebo-controlled trial was conducted comparing the effects of a cream containing Aloe vera versus a placebo cream on posthemorrhoidectomy pain. The study preparations were applied by patients to the surgical site 3 times per day for 4 weeks after hemorrhoidectomy. Pain was assessed with a visual analog scale immediately postoperatively and at hours 12, 24, and 48 after surgery and at weeks 2 and 4. Wound healing was examined and evaluated at the end of 2 and 4 weeks. The use of analgesics was recorded. Results: Forty-nine (49) patients were randomly assigned to receive aloe (n ¼ 24) or placebo (n ¼ 25). Patients in the topical aloe cream group had significantly less postoperative pain at hours 12, 24, and 48 hours and at 2 weeks. Aloe cream reduced the pain after defecation in 24 and 48 hours postsurgery (p < 0.001). Wound healing at the end of the second postoperative week was significantly greater in the aloe group compared with the placebo group (p < 0.001). Patients required fewer additional analgesics posthemorrhoidectomy (p < 0.001). Conclusions: Application of Aloe vera cream on the surgical site is effective in reducing postoperative pain both on resting and during defecation, healing time, and analgesic requirements in the patients compared with the placebo group.