Usman Shafqat - Academia.edu (original) (raw)
Papers by Usman Shafqat
Research Square (Research Square), Jun 3, 2024
Background Cotton Leaf Curl Virus Disease (CLCuD) is one of the major concerns for cotton growers... more Background Cotton Leaf Curl Virus Disease (CLCuD) is one of the major concerns for cotton growers. The traditional approach to managing CLCuD involves the control of the vector (white y) population through the use of pesticides. In this study, the e cacy of nanoparticles was compared with pesticides. The present study was conducted to evaluate the comparative e cacy of zinc oxide nanoparticles (ZnO nanoparticles), iron oxide nanoparticles (FeO nanoparticles), copper nanoparticles (Cu nanoparticles) and silver nanoparticles (Ag nanoparticles). Optimized doses of zinc oxide nanoparticles (ZnO nanoparticles), iron oxide nanoparticles (FeO nanoparticles), copper nanoparticles (Cu nanoparticles) and silver nanoparticles (Ag nanoparticles) were applied in a eld trial of cotton against Cotton Leaf Curl Virus Disease (CLCuD) in cotton. The study consisted of morphological parameters (height of stem, monopodial branches, sympodial branches, staple length, boll weight and number of bolls), yield parameters (seed cotton yield and ginning outturn), chlorophyll content (chlorophyll a, chlorophyll b, carotenoids and total chlorophyll), biochemical parameters (superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), hydrogen peroxide (H 2 O 2) and electrolyte leakage) and disease parameters (reduction infection, disease severity and disease incidence). Results Cotton Leaf Curl Virus (CLCuV) was detected by TAS-ELISA (Triple Antibody Sandwich-Enzyme-linked immunosorbent assay). Pesticide reduced the infection as 79.3%. Zinc oxide nanoparticles (ZnO nanoparticles), iron oxide nanoparticles (FeO nanoparticles), copper nanoparticles (Cu nanoparticles) and silver nanoparticles (Ag nanoparticles) reduced the infection as 42.33%, 41%, 34.7% and 44.8% respectively. The statistical design for eld trial was randomized complete block design (RCBD). Oneway ANOVA was performed.
Italian journal of food safety, Jan 26, 2024
This study examines the challenges Pakistani farmers face in adopting global good agricultural pr... more This study examines the challenges Pakistani farmers face in adopting global good agricultural practices (GGAP) and highlights the limitations in infrastructure and cost-based clauses. A questionnaire based on GGAP's fruit and vegetable module version 5.0 was developed and validated by the Department of Environmental Sciences, Government College University, Faisalabad. This was a survey-based study of 15 farmers divided into 5 groups according to their annual farm turnover. The findings of the study indicated that, although the basic paperwork requirements of GGAP were implementable, clauses related to capital investment and technical record-keeping were not. Results showed that 90-100% of farmers considered risk assessments, training, and documentation on their farms. However, 42-56% of clauses related to record-keeping, installation, visual presentation, and infrastructure development, and 24-37% of clauses related to external testing, health, safety, and hygiene were declared not implementable. The study revealed a need for adapting GGAP standards to Pakistan's unique agricultural conditions, suggesting the development of localized standards for more practical implementation. The study's findings highlight crucial insights for policymakers and stakeholders in the agriculture sector and suggest the need for target strategies to overcome implementation barriers and optimize the adaptation of Global GAP in Pakistan that would help to increase exports of agricultural commodities.
Sustainability, Oct 16, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Notulae Botanicae Horti Agrobotanici Cluj-napoca, Sep 26, 2023
Chemical and Biological Technologies in Agriculture
Background A pot experiment was conducted to evaluate the phytotoxic thresholds of zinc oxide (Zn... more Background A pot experiment was conducted to evaluate the phytotoxic thresholds of zinc oxide (ZnO NPs), iron oxide (FeO NPs), copper (Cu NPs), and silver nanoparticles (Ag NPs) on cotton. All the nanoparticles were biosynthesized from Conocarpus erectus L. leaf extract. They were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transmission infrared spectroscopy (FTIR). Five concentrations (0, 25, 50, 75, and 100 ppm) of all the biosynthesized nanoparticles were foliar applied thrice over the duration of cotton growth. Five cotton seeds were sown in 5 kg soil pot and harvested after 6 weeks. SPAD values were evaluated before harvesting while physical growth parameters, physiological parameters biochemical, and oxidative stress parameters were measured after harvesting. Results The value of all parameters, except oxidative stress, increased by 13–47% in response to ZnO NPs application up to 100 ppm. However, phytotoxic threshold of iron oxide...
The study aims to prepare reliable, ecofriendly and cost efficient zinc nanoparticles (Zn NPs) by... more The study aims to prepare reliable, ecofriendly and cost efficient zinc nanoparticles (Zn NPs) by Mentha piperita L. leaf extract and zinc sulfate heptahydrate, for four pathogenic bacteria. Synthesized NPs were investigated by using X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Zeta Potential. Disc diffusion method was used to check their antibacterial activity. For synthesizing Zn NPs leaf extract was used as a biological reducing agent. During reaction, appearance of cloudy white solution due to reduction in the number of zinc ions, indicated the Zn NPs formation. XRD determined the average NPs size i.e. 9.8 nm. FTIR confirmed the presence of functional groups in the leaf extract sample. Spherical shape of the NPs was confirmed by the SEM analysis. Antibacterial activity of the Zn NPs was measured by the inhibition zones against various bacteria (E. coli, P. aeruginosa, S. aureus and S. pneumoniae). Inhibition zon...
Research Square (Research Square), Jun 3, 2024
Background Cotton Leaf Curl Virus Disease (CLCuD) is one of the major concerns for cotton growers... more Background Cotton Leaf Curl Virus Disease (CLCuD) is one of the major concerns for cotton growers. The traditional approach to managing CLCuD involves the control of the vector (white y) population through the use of pesticides. In this study, the e cacy of nanoparticles was compared with pesticides. The present study was conducted to evaluate the comparative e cacy of zinc oxide nanoparticles (ZnO nanoparticles), iron oxide nanoparticles (FeO nanoparticles), copper nanoparticles (Cu nanoparticles) and silver nanoparticles (Ag nanoparticles). Optimized doses of zinc oxide nanoparticles (ZnO nanoparticles), iron oxide nanoparticles (FeO nanoparticles), copper nanoparticles (Cu nanoparticles) and silver nanoparticles (Ag nanoparticles) were applied in a eld trial of cotton against Cotton Leaf Curl Virus Disease (CLCuD) in cotton. The study consisted of morphological parameters (height of stem, monopodial branches, sympodial branches, staple length, boll weight and number of bolls), yield parameters (seed cotton yield and ginning outturn), chlorophyll content (chlorophyll a, chlorophyll b, carotenoids and total chlorophyll), biochemical parameters (superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), hydrogen peroxide (H 2 O 2) and electrolyte leakage) and disease parameters (reduction infection, disease severity and disease incidence). Results Cotton Leaf Curl Virus (CLCuV) was detected by TAS-ELISA (Triple Antibody Sandwich-Enzyme-linked immunosorbent assay). Pesticide reduced the infection as 79.3%. Zinc oxide nanoparticles (ZnO nanoparticles), iron oxide nanoparticles (FeO nanoparticles), copper nanoparticles (Cu nanoparticles) and silver nanoparticles (Ag nanoparticles) reduced the infection as 42.33%, 41%, 34.7% and 44.8% respectively. The statistical design for eld trial was randomized complete block design (RCBD). Oneway ANOVA was performed.
Italian journal of food safety, Jan 26, 2024
This study examines the challenges Pakistani farmers face in adopting global good agricultural pr... more This study examines the challenges Pakistani farmers face in adopting global good agricultural practices (GGAP) and highlights the limitations in infrastructure and cost-based clauses. A questionnaire based on GGAP's fruit and vegetable module version 5.0 was developed and validated by the Department of Environmental Sciences, Government College University, Faisalabad. This was a survey-based study of 15 farmers divided into 5 groups according to their annual farm turnover. The findings of the study indicated that, although the basic paperwork requirements of GGAP were implementable, clauses related to capital investment and technical record-keeping were not. Results showed that 90-100% of farmers considered risk assessments, training, and documentation on their farms. However, 42-56% of clauses related to record-keeping, installation, visual presentation, and infrastructure development, and 24-37% of clauses related to external testing, health, safety, and hygiene were declared not implementable. The study revealed a need for adapting GGAP standards to Pakistan's unique agricultural conditions, suggesting the development of localized standards for more practical implementation. The study's findings highlight crucial insights for policymakers and stakeholders in the agriculture sector and suggest the need for target strategies to overcome implementation barriers and optimize the adaptation of Global GAP in Pakistan that would help to increase exports of agricultural commodities.
Sustainability, Oct 16, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Notulae Botanicae Horti Agrobotanici Cluj-napoca, Sep 26, 2023
Chemical and Biological Technologies in Agriculture
Background A pot experiment was conducted to evaluate the phytotoxic thresholds of zinc oxide (Zn... more Background A pot experiment was conducted to evaluate the phytotoxic thresholds of zinc oxide (ZnO NPs), iron oxide (FeO NPs), copper (Cu NPs), and silver nanoparticles (Ag NPs) on cotton. All the nanoparticles were biosynthesized from Conocarpus erectus L. leaf extract. They were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transmission infrared spectroscopy (FTIR). Five concentrations (0, 25, 50, 75, and 100 ppm) of all the biosynthesized nanoparticles were foliar applied thrice over the duration of cotton growth. Five cotton seeds were sown in 5 kg soil pot and harvested after 6 weeks. SPAD values were evaluated before harvesting while physical growth parameters, physiological parameters biochemical, and oxidative stress parameters were measured after harvesting. Results The value of all parameters, except oxidative stress, increased by 13–47% in response to ZnO NPs application up to 100 ppm. However, phytotoxic threshold of iron oxide...
The study aims to prepare reliable, ecofriendly and cost efficient zinc nanoparticles (Zn NPs) by... more The study aims to prepare reliable, ecofriendly and cost efficient zinc nanoparticles (Zn NPs) by Mentha piperita L. leaf extract and zinc sulfate heptahydrate, for four pathogenic bacteria. Synthesized NPs were investigated by using X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Zeta Potential. Disc diffusion method was used to check their antibacterial activity. For synthesizing Zn NPs leaf extract was used as a biological reducing agent. During reaction, appearance of cloudy white solution due to reduction in the number of zinc ions, indicated the Zn NPs formation. XRD determined the average NPs size i.e. 9.8 nm. FTIR confirmed the presence of functional groups in the leaf extract sample. Spherical shape of the NPs was confirmed by the SEM analysis. Antibacterial activity of the Zn NPs was measured by the inhibition zones against various bacteria (E. coli, P. aeruginosa, S. aureus and S. pneumoniae). Inhibition zon...