Lata Shahani - Academia.edu (original) (raw)

Papers by Lata Shahani

Asian Pacific Journal of Reproduction, 2023

Materials Today: Proceedings

Toxicology International

Fenvalerate is a synthetic pyrethroid insecticide with moderate mammalian toxicity. It affects th... more Fenvalerate is a synthetic pyrethroid insecticide with moderate mammalian toxicity. It affects the central nervous system and is used for the control of flies and ticks in barns and stables. Exposure of this insecticide to humans occurs in the field. The present study was conducted to observe the toxicity of insecticide on biochemical and blood parameters after treatment with fenvalerate, a synthetic pyrethroid and find out the possible protective role of Quinoa seeds in Swiss albino mice. The mice in this study were divided into 5 groups, and 6 animals were taken into each group. Group A control mice received vehicle distilled water only. Group B and C were administered with fenvalerate at 12.5 and 25 mg/kg body weight respectively for 30 days. Group D was administered 12.5 mg/kg fenvalerate +25 mg/kg Quinoa seeds and Group E received 25 mg/kg fenvalerate +50 mg/kg Quinoa seeds. Fenvalerate treatment resulted in a significant increase in the levels of alkaline phosphatase, acid pho...

With the Increased cases of fungal infections which affects majority of population at different s... more With the Increased cases of fungal infections which affects majority of population at different stages and situations, one such section of population is of pregnant females. Rate and susceptibility of fungal infections is particularly higher in pregnant females as the immunity of the mother is compromised. Infections like Invasive aspergillosis, esophageal candidiasis and candidemia are being treated with new age triazole antifungals- voriconazole. Prolonged and high concentrations of this drug is associated with various developmental anomalies. With this aim teratogenic studies were performed on pregnant female mice during gestation and weaning period to observe the effects of voriconazole at different concentrations (8mg/kgb.w., 10mg/kg b.w., and 20mg/kg b.w.). Pregnant dams subjected to 20mg/kg b.w. voriconazole had small litter size and high resorptions. Presence of 14 th rib, asymmetry in sternebrae and craniofacial defects were some of skeletal anomalies in foetus and pups sub...

Journal of research in pharmacy, 2023

Materials Today: Proceedings

Iranian Journal of Applied Animal Science, 2015

We studied the hepatotoxic effects of a deltamethrin containing formulation (Decis®) on developin... more We studied the hepatotoxic effects of a deltamethrin containing formulation (Decis®) on developing embryo of Gallus domesticus. Fertilized eggs were immersed in three different concentrations of deltamethrin (12.5, 25 and 50 mg/L) for 60 min at 37 ˚C prior to incubation (ED 0) and on the 4 th day of incubation (ED 4) and incubated till embryonic day (ED) 16. Exposure to 50 mg/L of deltamethrin resulted in a significant decrease in total protein and total glycogen content in the liver when exposed on ED 4 and ED 0 respectively, while total glutathione content was reduced at concentrations applied on both the exposure days. Among enzymes, liver alkaline phosphatase activity was significantly increased at 25 and 50 mg/L deltamethrin when embryos were exposed on ED 0 and 4, whereas the glutamate pyruvate transaminase activity showed a marked increase in animals exposed to 50 mg/L deltamethrin only at ED 4. Histophathological evaluation revealed mild to marked cellular lesions in liver s...

The most common source of human exposure to fluoride and aluminium include drinking water and var... more The most common source of human exposure to fluoride and aluminium include drinking water and various food items. Both fluoride and aluminium have been reported to induce oxidative stress mediated neurodegeneration. The aim of the present study is to evaluate the protective effect of Nigella sativa and deferrioxamine against combination of fluoride aluminium induced toxicity in brain of male Swiss albino mice. Mice were treated intraperitoneally with fluoride and aluminium for one month (thrice a week on alternate days) which resulted in decrease in various antioxidant enzymes like SOD, catalase, GPx, GSH and increase in LPO levels. Neurodegenerataive changes were also observed in histology of brain. DNA fragmentation and increased GFAP and Caspase 3 expression was also observed indicating that combination of fluoride and aluminium leads to oxidative stress mediated apoptosis in brain cells. Treatment with Nigella sativa and deferrioxamine resulted in amelioration of neurodegenerati...

The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin we... more The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin were examined using those commercial formulations which are used extensively throughout in India for the agricultural purposes. Fertilized eggs were immersed in various dose concentrations of each insecticide for 60 min at 37 ° C on prior to incubation, 4 th and 7 th day of incubation. The applied concentrations of each insecticide were 12.5, 25 and 50 mg L-1 of deltamethrin formulation and 250, 500 and 1000 mg L-1 of dicofol formulation. Sampling of all the treated embryos was done on embryonic day 16. The result revealed that administration of deltamethrin prior to incubation showed significant increase in percent mortality at concentrations of 25 mg L-1 and 50mg L-1 when compared with controls. No significant (p≥0.05) increases in percent mortality were observed in embryos treated with these insecticides on 4 th and 7 th day of incubation. Further, brain AChE activity of treated embryos...

Pesticides are major contaminants of our environment and pose a serious threat to non target orga... more Pesticides are major contaminants of our environment and pose a serious threat to non target organisms. The present experimental design aims to study the toxic effect of Flash on the embryo of chick treated at the post organogenesis stage of its developmental period. Fertilized eggs of pure breed (BV 300) of chick were immersed in low, moderate and high concentrations of the toxicant for one hour on day 7 of incubation and recovered on day 15 to observe skeletal development. The treated group’s revealed malformations at all doses. The mortality rate was found to increase with significant decrease in wet body weight with increase in the dose. The pesticide caused overall reduction in ossification of skeleton; with significant malformations at only high dose, and in thoracic and caudal vertebrae. Present findings show that Flash is a potent teratogenic with reference to avian species.

To investigate the protective role of broccoli powder “ against continuous ingestion of escitalop... more To investigate the protective role of broccoli powder “ against continuous ingestion of escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice. Methods: Mice were divided into different groups. Group1: Normal control (0.9% NaCl), Group 2: Escitalopram drug trea 3: Broccoli powder with Escitalopram drug treated (200 mg/kg + 20 mg/kg), Group 4: Olive oil vehicle control, Group 5: Carbon tetrachloride (CCl4) referenced as positive control (33 mg/kg), Group 6: Broccoli powder with these groups on liver tissue was studied after three different time periods weeks. increased serum SGO SOD and GSH) that are comparable with CCl4 intoxicated group considered as positive control. Comparing escitalopram drug treated group with group that received both broccoli po levels and restored the level of antioxidant enzymes. The protective effect of broccoli powder on escitalopram drug induced hepatotoxicity was also supported by histopa

The teratogenicity of a commercial formulation of the insecticide deltamethrin (Decis ® ) in chic... more The teratogenicity of a commercial formulation of the insecticide deltamethrin (Decis ® ) in chick embryos was evaluated. Fertilized eggs of Gallus domesticus were immersed in aqueous emulsions of deltamethrin at concentrations of 12.5 mg L -1 , 25 mg L -1 and 50 mg L -1 for 60 min at 37 ◦ C on 4 th day of incubation. Two control groups of eggs were used: One group was immersed in distilled water (vehicle) and the second group was kept as untreated to study background toxicity. On embryonic day 16; recovered embryos were evaluated for mortality rate, wet body weight, gross morphological and skeletal malformations. The result revealed that embryonic mortality markedly increased after administration of deltamethrin. The significant decrease (p≤0.05) in wet body weight and significant increase (p≤0.05) in percentage of abnormal survivors was observed in dose dependent manner. A spectrum of external and skeletal malformations was exhibited by deltamethrin treated embryos. These finding ...

Human and Ecological Risk Assessment: An International Journal, 2015

ABSTRACT A deltamethrin containing insecticide formulation (Decis®) was evaluated for its toxic p... more ABSTRACT A deltamethrin containing insecticide formulation (Decis®) was evaluated for its toxic potential in developing chick embryos. For the present study, three water emulsified concentrations of Decis® (12.5 mg L−1, 25 mg L−1, and 50 mg L−1) were used. Fertilized eggs of Gallus domesticus were immersed in these three concentrations of the insecticide for 60 min at 37°C on day 0 of incubation and kept for incubation till embryonic day 7. Recovered embryos were evaluated for teratogenic and biochemical changes. The results revealed that administration of Decis® at its lower concentrations (12.5 mg L−1 and 25 mg L−1) did not show any significant teratological changes but the significant number of abnormal survivors was observed at 50 mg L−1 of dose concentration when compared with vehicle-treated control. Among biochemical changes, total glycogen and RNA contents of embryos was significantly decreased at 25 mg L−1 and 50 mg L−1 of Decis® concentrations. Similarly, significant alteration (p ≤ .05) was observed in alanine transaminase activity at 50 mg L−1 concentration of Decis®. Thus, the present study concluded that the no-effect-level for developmental toxicity for Decis® is below the concentration of 25 mg L−1 under standard laboratory conditions.

Present study was designed to investigate the toxic effects of Carbosulfan 25% EC Marshal on the ... more Present study was designed to investigate the toxic effects of Carbosulfan 25% EC Marshal on the developing chick embryo at critical period of its developmental stage. Fertilized eggs of pure breed of chick (BV 300) were immersed in low, medium and sub lethal concentrations of the toxicant for 60 minutes on day 4 of incubation. The gross morphological and skeletal features were studied on day 10 and day 15 of the incubation period. The pesticide caused musculoskeletal deformities in skin, limbs, head, neck, skull, lower body and overall reduction in ossification of skeleton. The embryolethality percentage was highest at the highest dose and lower in the lowest dose. However, incidence of malformations was found to be significant and showed increase with the increasing concentrations of the toxicant. Thus, insecticide Marshal EC with active ingredient Carbosulfan is a potent teratogen with reference to avian systems.

L -1 of Colonel-S ® exposed on ED 4 and ED 7, respectively. A significant increase in the percent... more L -1 of Colonel-S ® exposed on ED 4 and ED 7, respectively. A significant increase in the percentage of abnormal survivors exhibiting a spectrum of morphological as well as skeletal malformations was observed in each of the insecticide treated group. These findings suggest that Colonel-S ® and Decis ® can be considered as teratogenic agents, at the dose levels used at our laboratory conditions.

International Research Journal of Pharmacy

Antidepressants induced metabolic syndrome is a major public health problem in the treatment of p... more Antidepressants induced metabolic syndrome is a major public health problem in the treatment of psychiatric disorders which leads to an increased risk of cardiovascular disease, non-alcoholic hepatic steatosis and renal dysfunction. Psychiatric disorder is a mental illness that includes depression, schizophrenia, and anxiety disorder that affect millions of people around the world. The purpose of present study was to investigate the protective role of broccoli powder against continuous ingestion of antidepressant drug induced nephrotoxicity in Swiss albino mice. In present study mice were continuously treated with escitalopram antidepressant drug (20 mg/kg/b.w. orally) for 4, 8 and 12 weeks. The escitalopram drug intoxicated group showed elevation in serum urea and creatinine level. Escitalopram drug intoxicated group also showed degeneration of glomerulus and renal tubule that are comparable with carbon tetrachloride (33 mg/kg) group considered as positive control. Group that received both broccoli powder and escitalopram drug (200 mg/kg + 20 mg/kg) significantly reduced elevated level of serum urea and creatinine with mild degeneration of glomerulus and renal tubule as compared to antidepressant drug intoxicated group.

Toxicological & Environmental Chemistry, 2014

In the present investigation, hepatotoxic effect of a commercially available insecticide formulat... more In the present investigation, hepatotoxic effect of a commercially available insecticide formulation of dicofol (Colonel–S® 18.5% emulsified concentrate) was studied in the developing chick embryo. Fertilized eggs of Gallus domesticus were immersed in three different dose concentrations, i.e. 250, 500, and 1000 mg L−1 of Colonel–S on “0” and fourth days of incubation for 60 min at 37 °C and incubated till embryonic day 16. Severe histopathological cellular lesions, such as extensive cell degeneration and necrosis with enlarged blood sinusoids, cytoplasmic vacuolization, and leucocyte infiltrations with congestion or dilation of central vein, appeared in dose-depended manner. Dicofol treatment also caused significant decrease in the levels of total protein, glycogen, and glutathione content and an increase in alkaline phosphatase activity of embryonic liver, whereas glutamic pyruvic transaminase activity showed mixed response.

The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin we... more The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin were examined using those commercial formulations which are used extensively throughout in India for the agricultural purposes. Fertilized eggs were immersed in various dose concentrations of each insecticide for 60 min at 37 ° C on prior to incubation, 4 th and 7 th day of incubation. The applied concentrations of each insecticide were 12.5, 25 and 50 mg L-1 of deltamethrin formulation and 250, 500 and 1000 mg L-1 of dicofol formulation. Sampling of all the treated embryos was done on embryonic day 16. The result revealed that administration of deltamethrin prior to incubation showed significant increase in percent mortality at concentrations of 25 mg L-1 and 50mg L-1 when compared with controls. No significant (p≥0.05) increases in percent mortality were observed in embryos treated with these insecticides on 4 th and 7 th day of incubation. Further, brain AChE activity of treated embryos...

The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin we... more The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin were examined using those commercial formulations which are used extensively throughout in India for the agricultural purposes. Fertilized eggs were immersed in various dose concentrations of each insecticide for 60 min at 37 ° C on prior to incubation, 4 th and 7 th day of incubation. The applied concentrations of each insecticide were 12.5, 25 and 50 mg L -1 of deltamethrin formulation and 250, 500 and 1000 mg L -1 of dicofol formulation. Sampling of all the treated embryos was done on embryonic day 16. The result revealed that administration of deltamethrin prior to incubation showed significant increase in percent mortality at concentrations of 25 mg L -1 and 50mg L -1 when compared with controls. No significant (p≥0.05) increases in percent mortality were observed in embryos treated with these insecticides on 4 th and 7 th day of incubation. Further, brain AChE activity of treated emb...

Asian Pacific Journal of Reproduction, 2023

Materials Today: Proceedings

Toxicology International

Fenvalerate is a synthetic pyrethroid insecticide with moderate mammalian toxicity. It affects th... more Fenvalerate is a synthetic pyrethroid insecticide with moderate mammalian toxicity. It affects the central nervous system and is used for the control of flies and ticks in barns and stables. Exposure of this insecticide to humans occurs in the field. The present study was conducted to observe the toxicity of insecticide on biochemical and blood parameters after treatment with fenvalerate, a synthetic pyrethroid and find out the possible protective role of Quinoa seeds in Swiss albino mice. The mice in this study were divided into 5 groups, and 6 animals were taken into each group. Group A control mice received vehicle distilled water only. Group B and C were administered with fenvalerate at 12.5 and 25 mg/kg body weight respectively for 30 days. Group D was administered 12.5 mg/kg fenvalerate +25 mg/kg Quinoa seeds and Group E received 25 mg/kg fenvalerate +50 mg/kg Quinoa seeds. Fenvalerate treatment resulted in a significant increase in the levels of alkaline phosphatase, acid pho...

With the Increased cases of fungal infections which affects majority of population at different s... more With the Increased cases of fungal infections which affects majority of population at different stages and situations, one such section of population is of pregnant females. Rate and susceptibility of fungal infections is particularly higher in pregnant females as the immunity of the mother is compromised. Infections like Invasive aspergillosis, esophageal candidiasis and candidemia are being treated with new age triazole antifungals- voriconazole. Prolonged and high concentrations of this drug is associated with various developmental anomalies. With this aim teratogenic studies were performed on pregnant female mice during gestation and weaning period to observe the effects of voriconazole at different concentrations (8mg/kgb.w., 10mg/kg b.w., and 20mg/kg b.w.). Pregnant dams subjected to 20mg/kg b.w. voriconazole had small litter size and high resorptions. Presence of 14 th rib, asymmetry in sternebrae and craniofacial defects were some of skeletal anomalies in foetus and pups sub...

Journal of research in pharmacy, 2023

Materials Today: Proceedings

Iranian Journal of Applied Animal Science, 2015

We studied the hepatotoxic effects of a deltamethrin containing formulation (Decis®) on developin... more We studied the hepatotoxic effects of a deltamethrin containing formulation (Decis®) on developing embryo of Gallus domesticus. Fertilized eggs were immersed in three different concentrations of deltamethrin (12.5, 25 and 50 mg/L) for 60 min at 37 ˚C prior to incubation (ED 0) and on the 4 th day of incubation (ED 4) and incubated till embryonic day (ED) 16. Exposure to 50 mg/L of deltamethrin resulted in a significant decrease in total protein and total glycogen content in the liver when exposed on ED 4 and ED 0 respectively, while total glutathione content was reduced at concentrations applied on both the exposure days. Among enzymes, liver alkaline phosphatase activity was significantly increased at 25 and 50 mg/L deltamethrin when embryos were exposed on ED 0 and 4, whereas the glutamate pyruvate transaminase activity showed a marked increase in animals exposed to 50 mg/L deltamethrin only at ED 4. Histophathological evaluation revealed mild to marked cellular lesions in liver s...

The most common source of human exposure to fluoride and aluminium include drinking water and var... more The most common source of human exposure to fluoride and aluminium include drinking water and various food items. Both fluoride and aluminium have been reported to induce oxidative stress mediated neurodegeneration. The aim of the present study is to evaluate the protective effect of Nigella sativa and deferrioxamine against combination of fluoride aluminium induced toxicity in brain of male Swiss albino mice. Mice were treated intraperitoneally with fluoride and aluminium for one month (thrice a week on alternate days) which resulted in decrease in various antioxidant enzymes like SOD, catalase, GPx, GSH and increase in LPO levels. Neurodegenerataive changes were also observed in histology of brain. DNA fragmentation and increased GFAP and Caspase 3 expression was also observed indicating that combination of fluoride and aluminium leads to oxidative stress mediated apoptosis in brain cells. Treatment with Nigella sativa and deferrioxamine resulted in amelioration of neurodegenerati...

The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin we... more The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin were examined using those commercial formulations which are used extensively throughout in India for the agricultural purposes. Fertilized eggs were immersed in various dose concentrations of each insecticide for 60 min at 37 ° C on prior to incubation, 4 th and 7 th day of incubation. The applied concentrations of each insecticide were 12.5, 25 and 50 mg L-1 of deltamethrin formulation and 250, 500 and 1000 mg L-1 of dicofol formulation. Sampling of all the treated embryos was done on embryonic day 16. The result revealed that administration of deltamethrin prior to incubation showed significant increase in percent mortality at concentrations of 25 mg L-1 and 50mg L-1 when compared with controls. No significant (p≥0.05) increases in percent mortality were observed in embryos treated with these insecticides on 4 th and 7 th day of incubation. Further, brain AChE activity of treated embryos...

Pesticides are major contaminants of our environment and pose a serious threat to non target orga... more Pesticides are major contaminants of our environment and pose a serious threat to non target organisms. The present experimental design aims to study the toxic effect of Flash on the embryo of chick treated at the post organogenesis stage of its developmental period. Fertilized eggs of pure breed (BV 300) of chick were immersed in low, moderate and high concentrations of the toxicant for one hour on day 7 of incubation and recovered on day 15 to observe skeletal development. The treated group’s revealed malformations at all doses. The mortality rate was found to increase with significant decrease in wet body weight with increase in the dose. The pesticide caused overall reduction in ossification of skeleton; with significant malformations at only high dose, and in thoracic and caudal vertebrae. Present findings show that Flash is a potent teratogenic with reference to avian species.

To investigate the protective role of broccoli powder “ against continuous ingestion of escitalop... more To investigate the protective role of broccoli powder “ against continuous ingestion of escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice. Methods: Mice were divided into different groups. Group1: Normal control (0.9% NaCl), Group 2: Escitalopram drug trea 3: Broccoli powder with Escitalopram drug treated (200 mg/kg + 20 mg/kg), Group 4: Olive oil vehicle control, Group 5: Carbon tetrachloride (CCl4) referenced as positive control (33 mg/kg), Group 6: Broccoli powder with these groups on liver tissue was studied after three different time periods weeks. increased serum SGO SOD and GSH) that are comparable with CCl4 intoxicated group considered as positive control. Comparing escitalopram drug treated group with group that received both broccoli po levels and restored the level of antioxidant enzymes. The protective effect of broccoli powder on escitalopram drug induced hepatotoxicity was also supported by histopa

The teratogenicity of a commercial formulation of the insecticide deltamethrin (Decis ® ) in chic... more The teratogenicity of a commercial formulation of the insecticide deltamethrin (Decis ® ) in chick embryos was evaluated. Fertilized eggs of Gallus domesticus were immersed in aqueous emulsions of deltamethrin at concentrations of 12.5 mg L -1 , 25 mg L -1 and 50 mg L -1 for 60 min at 37 ◦ C on 4 th day of incubation. Two control groups of eggs were used: One group was immersed in distilled water (vehicle) and the second group was kept as untreated to study background toxicity. On embryonic day 16; recovered embryos were evaluated for mortality rate, wet body weight, gross morphological and skeletal malformations. The result revealed that embryonic mortality markedly increased after administration of deltamethrin. The significant decrease (p≤0.05) in wet body weight and significant increase (p≤0.05) in percentage of abnormal survivors was observed in dose dependent manner. A spectrum of external and skeletal malformations was exhibited by deltamethrin treated embryos. These finding ...

Human and Ecological Risk Assessment: An International Journal, 2015

ABSTRACT A deltamethrin containing insecticide formulation (Decis®) was evaluated for its toxic p... more ABSTRACT A deltamethrin containing insecticide formulation (Decis®) was evaluated for its toxic potential in developing chick embryos. For the present study, three water emulsified concentrations of Decis® (12.5 mg L−1, 25 mg L−1, and 50 mg L−1) were used. Fertilized eggs of Gallus domesticus were immersed in these three concentrations of the insecticide for 60 min at 37°C on day 0 of incubation and kept for incubation till embryonic day 7. Recovered embryos were evaluated for teratogenic and biochemical changes. The results revealed that administration of Decis® at its lower concentrations (12.5 mg L−1 and 25 mg L−1) did not show any significant teratological changes but the significant number of abnormal survivors was observed at 50 mg L−1 of dose concentration when compared with vehicle-treated control. Among biochemical changes, total glycogen and RNA contents of embryos was significantly decreased at 25 mg L−1 and 50 mg L−1 of Decis® concentrations. Similarly, significant alteration (p ≤ .05) was observed in alanine transaminase activity at 50 mg L−1 concentration of Decis®. Thus, the present study concluded that the no-effect-level for developmental toxicity for Decis® is below the concentration of 25 mg L−1 under standard laboratory conditions.

Present study was designed to investigate the toxic effects of Carbosulfan 25% EC Marshal on the ... more Present study was designed to investigate the toxic effects of Carbosulfan 25% EC Marshal on the developing chick embryo at critical period of its developmental stage. Fertilized eggs of pure breed of chick (BV 300) were immersed in low, medium and sub lethal concentrations of the toxicant for 60 minutes on day 4 of incubation. The gross morphological and skeletal features were studied on day 10 and day 15 of the incubation period. The pesticide caused musculoskeletal deformities in skin, limbs, head, neck, skull, lower body and overall reduction in ossification of skeleton. The embryolethality percentage was highest at the highest dose and lower in the lowest dose. However, incidence of malformations was found to be significant and showed increase with the increasing concentrations of the toxicant. Thus, insecticide Marshal EC with active ingredient Carbosulfan is a potent teratogen with reference to avian systems.

L -1 of Colonel-S ® exposed on ED 4 and ED 7, respectively. A significant increase in the percent... more L -1 of Colonel-S ® exposed on ED 4 and ED 7, respectively. A significant increase in the percentage of abnormal survivors exhibiting a spectrum of morphological as well as skeletal malformations was observed in each of the insecticide treated group. These findings suggest that Colonel-S ® and Decis ® can be considered as teratogenic agents, at the dose levels used at our laboratory conditions.

International Research Journal of Pharmacy

Antidepressants induced metabolic syndrome is a major public health problem in the treatment of p... more Antidepressants induced metabolic syndrome is a major public health problem in the treatment of psychiatric disorders which leads to an increased risk of cardiovascular disease, non-alcoholic hepatic steatosis and renal dysfunction. Psychiatric disorder is a mental illness that includes depression, schizophrenia, and anxiety disorder that affect millions of people around the world. The purpose of present study was to investigate the protective role of broccoli powder against continuous ingestion of antidepressant drug induced nephrotoxicity in Swiss albino mice. In present study mice were continuously treated with escitalopram antidepressant drug (20 mg/kg/b.w. orally) for 4, 8 and 12 weeks. The escitalopram drug intoxicated group showed elevation in serum urea and creatinine level. Escitalopram drug intoxicated group also showed degeneration of glomerulus and renal tubule that are comparable with carbon tetrachloride (33 mg/kg) group considered as positive control. Group that received both broccoli powder and escitalopram drug (200 mg/kg + 20 mg/kg) significantly reduced elevated level of serum urea and creatinine with mild degeneration of glomerulus and renal tubule as compared to antidepressant drug intoxicated group.

Toxicological & Environmental Chemistry, 2014

In the present investigation, hepatotoxic effect of a commercially available insecticide formulat... more In the present investigation, hepatotoxic effect of a commercially available insecticide formulation of dicofol (Colonel–S® 18.5% emulsified concentrate) was studied in the developing chick embryo. Fertilized eggs of Gallus domesticus were immersed in three different dose concentrations, i.e. 250, 500, and 1000 mg L−1 of Colonel–S on “0” and fourth days of incubation for 60 min at 37 °C and incubated till embryonic day 16. Severe histopathological cellular lesions, such as extensive cell degeneration and necrosis with enlarged blood sinusoids, cytoplasmic vacuolization, and leucocyte infiltrations with congestion or dilation of central vein, appeared in dose-depended manner. Dicofol treatment also caused significant decrease in the levels of total protein, glycogen, and glutathione content and an increase in alkaline phosphatase activity of embryonic liver, whereas glutamic pyruvic transaminase activity showed mixed response.

The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin we... more The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin were examined using those commercial formulations which are used extensively throughout in India for the agricultural purposes. Fertilized eggs were immersed in various dose concentrations of each insecticide for 60 min at 37 ° C on prior to incubation, 4 th and 7 th day of incubation. The applied concentrations of each insecticide were 12.5, 25 and 50 mg L-1 of deltamethrin formulation and 250, 500 and 1000 mg L-1 of dicofol formulation. Sampling of all the treated embryos was done on embryonic day 16. The result revealed that administration of deltamethrin prior to incubation showed significant increase in percent mortality at concentrations of 25 mg L-1 and 50mg L-1 when compared with controls. No significant (p≥0.05) increases in percent mortality were observed in embryos treated with these insecticides on 4 th and 7 th day of incubation. Further, brain AChE activity of treated embryos...

The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin we... more The effects of external exposure of chick (Gallus domesticus) eggs to dicofol and deltamethrin were examined using those commercial formulations which are used extensively throughout in India for the agricultural purposes. Fertilized eggs were immersed in various dose concentrations of each insecticide for 60 min at 37 ° C on prior to incubation, 4 th and 7 th day of incubation. The applied concentrations of each insecticide were 12.5, 25 and 50 mg L -1 of deltamethrin formulation and 250, 500 and 1000 mg L -1 of dicofol formulation. Sampling of all the treated embryos was done on embryonic day 16. The result revealed that administration of deltamethrin prior to incubation showed significant increase in percent mortality at concentrations of 25 mg L -1 and 50mg L -1 when compared with controls. No significant (p≥0.05) increases in percent mortality were observed in embryos treated with these insecticides on 4 th and 7 th day of incubation. Further, brain AChE activity of treated emb...