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Papers by Shail Singh

Research paper thumbnail of A case control study of clinical and biochemical parameters of metabolic syndrome with special attention among young and middle aged population

Diabetes & Metabolic Syndrome: Clinical Research & Reviews

Research paper thumbnail of Synergistic biodegradation of pentachlorophenol by Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385)

World Journal of Microbiology and Biotechnology, Jun 18, 2009

The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcesc... more The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple (BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains (62.75-90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC-MS analysis revealed the formation of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from degraded sample as compared to control.

Research paper thumbnail of Biodegradation of phenol in batch culture by pure and mixed strains of Paenibacillus sp. and Bacillus cereus

Polish Journal of Microbiology Polskie Towarzystwo Mikrobiologow the Polish Society of Microbiologists, 2009

Research paper thumbnail of Biodegradation of Phenol in Batch Culture by Pure and

Research paper thumbnail of Synergistic biodegradation of pentachlorophenol by Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385)

World Journal of …, 2009

The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcesc... more The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple (BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains (62.75-90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC-MS analysis revealed the formation of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from degraded sample as compared to control.

Research paper thumbnail of Investigation of the biotransformation of pentachlorophenol and pulp paper mill effluent decolorisation by the bacterial strains in a mixed culture

Bioresource technology, 2008

Mixed culture of two bacterial strains Bacillus sp. and Serratia marcescens showed potential pent... more Mixed culture of two bacterial strains Bacillus sp. and Serratia marcescens showed potential pentachlorophenol (PCP) degradation and decolorisation of pulp paper mill effluent. The physico-chemical quality of pulp paper mill effluent has been analyzed after 168 h incubation period degraded by mixed culture. The study revealed that it has decreased high load of BOD, COD, TS, TDS, TSS, sulphate, phosphate, total nitrogen, total phenols, metals and different salts (i.e. chloride, sodium, nitrate, potassium) at 168 h incubation period. PCP degradation in pulp paper mill effluent was confirmed by HPLC analysis. Mixed culture was found to degrade PCP up to (94%) present in pulp paper mill effluent with 1% glucose and 0.5% peptone (w/v) at 30+/-1 degrees C, pH 8.0+/-0.2 at 120 rpm in 168 h incubation period. The simultaneous release of chloride ion up to 1,200 mg/l at 168 h emphasized the bacterial dechlorination in the medium. The pulp paper mill effluent degradation was also supported by decline in pH, AOX (absorbable organic halides), color, D.O., BOD, COD and PCP. The analysis of pulp paper mill effluent degradation products by GC-MS analysis revealed the formation of low molecular weight compound like 2-chlorophenol (RT=3.8 min) and tetrachlorohydroquinone (RT=11.86 min) from PCP extracted degraded sample. Further, mixed culture may be used for bioremediation of PCP containing pulp paper mill waste in the environment.

Research paper thumbnail of Isolation and characterization of novel Serratia marcescens (AY927692) for pentachlorophenol degradation from pulp and paper mill waste

World Journal of Microbiology and …, 2007

Seven aerobic bacterial strains were isolated from pulp paper mill waste and screened for pentach... more Seven aerobic bacterial strains were isolated from pulp paper mill waste and screened for pentachlorophenol (PCP) tolerance on PCP containing mineral salt agar medium (MSM). The organism was characterized by 16S rDNA sequencing which showed 99.7% sequence similarity with Serratia marcescens. PCP degradation was routinely monitored with spectrophotometric analysis and further confirmed by HPLC analysis. Among seven strains, ITRC S 7 was found to degrade up to 90.33% of 1.127 mM (300 mg/l) of PCP and simultaneous release of chloride ion (2.435 mM) emphasized the bacterial dechlorination in the medium in presence of glucose as an additional carbon and energy source under optimized condition within 168 h incubation. In absence of glucose bacterium was unable to utilize PCP indicating the phenomenon of co-metabolism. Bacterium was identified as S. marcescens (AY927692), was a novel and potential aerobic bacterial strain capable of degrading PCP in axenic condition. Further, this strain may be used for bioremediation of PCP containing pulp paper mill waste in the environment.

Research paper thumbnail of Seasonal Bacteriological Analysis of Gola River Water Contaminated with Pulp Paper Mill Waste in Uttaranchal, India

Environmental Monitoring and Assessment, 2006

The seasonal physico-chemical and microbial quality of Gola river water has been analyzed after c... more The seasonal physico-chemical and microbial quality of Gola river water has been analyzed after confluence of pulp paper mill waste. The study revealed that it has enhanced 20–30 times pollution load of BOD, COD, TDS, TSS, sulphate, chloride, sodium, nitrate, potassium, lignin and phenol after mixing of pulp paper mill waste with river water in all season. Further, it induced the bacterial growth by increasing most probable number value of E. coli was 1.57 × 104, 1.6 × 104, 1.37 × 104and SPC count was 1.68 × 104, 1.64 × 104, 1.67 × 104/100 ml during summer, monsoon, winter respectively. While the most probable number value in river water before mixing of pulp paper mill waste was 1.4 × 102, 1.82 × 102, 1.5 × 102and SPC count was 2.8 × 103, 2.89 × 103, 2.78 × 103/100 ml during summer, monsoon and winter respectively. This indicated from 88 –114 fold increase in most probable number value of E. coli and 56.55 –60.0 times increase in SPC count of river water after mixing of effluent in...

Research paper thumbnail of Isolation and Characterization of Bacterial Strain for the Degradation of Phenol

Research paper thumbnail of Isolation and characterization of two potential pentachlorophenol degrading aerobic bacteria from pulp paper effluent sludge

The Journal of general and …, 2006

Chlorophenols (CPs) are major environmental pollutants discharged from pulp and paper mills, tann... more Chlorophenols (CPs) are major environmental pollutants discharged from pulp and paper mills, tanneries, distilleries, dye and paint manufacturing and pharmaceuticals industry (Crosby, 1981). The major source of CPs is as by-products when chlorine is used for bleaching of pulp and for disinfections of drinking water. Therefore, large amounts of chlorinated aromatic compounds, including CPs, have been discharged into the environment (Steinle et al., 1998). Among chlorophenols, pentachlorophenol (PCP) is a wide spectrum biocide with applications in agriculture, industry and public health (Bevenue and Beckman, 1967). PCP a highly toxic pesticide has been widely used in disinfectants and preservatives (Rao, 1978). The U.S. Environmental Protection Agency regulates PCP as a priority pollutant and also considers 1.0 mg/L of PCP hazardous for land disposal (Freeman, 1989; Sittig, 1981).

Research paper thumbnail of A case control study of clinical and biochemical parameters of metabolic syndrome with special attention among young and middle aged population

Diabetes & Metabolic Syndrome: Clinical Research & Reviews

Research paper thumbnail of Synergistic biodegradation of pentachlorophenol by Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385)

World Journal of Microbiology and Biotechnology, Jun 18, 2009

The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcesc... more The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple (BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains (62.75-90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC-MS analysis revealed the formation of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from degraded sample as compared to control.

Research paper thumbnail of Biodegradation of phenol in batch culture by pure and mixed strains of Paenibacillus sp. and Bacillus cereus

Polish Journal of Microbiology Polskie Towarzystwo Mikrobiologow the Polish Society of Microbiologists, 2009

Research paper thumbnail of Biodegradation of Phenol in Batch Culture by Pure and

Research paper thumbnail of Synergistic biodegradation of pentachlorophenol by Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385)

World Journal of …, 2009

The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcesc... more The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple (BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains (62.75-90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC-MS analysis revealed the formation of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from degraded sample as compared to control.

Research paper thumbnail of Investigation of the biotransformation of pentachlorophenol and pulp paper mill effluent decolorisation by the bacterial strains in a mixed culture

Bioresource technology, 2008

Mixed culture of two bacterial strains Bacillus sp. and Serratia marcescens showed potential pent... more Mixed culture of two bacterial strains Bacillus sp. and Serratia marcescens showed potential pentachlorophenol (PCP) degradation and decolorisation of pulp paper mill effluent. The physico-chemical quality of pulp paper mill effluent has been analyzed after 168 h incubation period degraded by mixed culture. The study revealed that it has decreased high load of BOD, COD, TS, TDS, TSS, sulphate, phosphate, total nitrogen, total phenols, metals and different salts (i.e. chloride, sodium, nitrate, potassium) at 168 h incubation period. PCP degradation in pulp paper mill effluent was confirmed by HPLC analysis. Mixed culture was found to degrade PCP up to (94%) present in pulp paper mill effluent with 1% glucose and 0.5% peptone (w/v) at 30+/-1 degrees C, pH 8.0+/-0.2 at 120 rpm in 168 h incubation period. The simultaneous release of chloride ion up to 1,200 mg/l at 168 h emphasized the bacterial dechlorination in the medium. The pulp paper mill effluent degradation was also supported by decline in pH, AOX (absorbable organic halides), color, D.O., BOD, COD and PCP. The analysis of pulp paper mill effluent degradation products by GC-MS analysis revealed the formation of low molecular weight compound like 2-chlorophenol (RT=3.8 min) and tetrachlorohydroquinone (RT=11.86 min) from PCP extracted degraded sample. Further, mixed culture may be used for bioremediation of PCP containing pulp paper mill waste in the environment.

Research paper thumbnail of Isolation and characterization of novel Serratia marcescens (AY927692) for pentachlorophenol degradation from pulp and paper mill waste

World Journal of Microbiology and …, 2007

Seven aerobic bacterial strains were isolated from pulp paper mill waste and screened for pentach... more Seven aerobic bacterial strains were isolated from pulp paper mill waste and screened for pentachlorophenol (PCP) tolerance on PCP containing mineral salt agar medium (MSM). The organism was characterized by 16S rDNA sequencing which showed 99.7% sequence similarity with Serratia marcescens. PCP degradation was routinely monitored with spectrophotometric analysis and further confirmed by HPLC analysis. Among seven strains, ITRC S 7 was found to degrade up to 90.33% of 1.127 mM (300 mg/l) of PCP and simultaneous release of chloride ion (2.435 mM) emphasized the bacterial dechlorination in the medium in presence of glucose as an additional carbon and energy source under optimized condition within 168 h incubation. In absence of glucose bacterium was unable to utilize PCP indicating the phenomenon of co-metabolism. Bacterium was identified as S. marcescens (AY927692), was a novel and potential aerobic bacterial strain capable of degrading PCP in axenic condition. Further, this strain may be used for bioremediation of PCP containing pulp paper mill waste in the environment.

Research paper thumbnail of Seasonal Bacteriological Analysis of Gola River Water Contaminated with Pulp Paper Mill Waste in Uttaranchal, India

Environmental Monitoring and Assessment, 2006

The seasonal physico-chemical and microbial quality of Gola river water has been analyzed after c... more The seasonal physico-chemical and microbial quality of Gola river water has been analyzed after confluence of pulp paper mill waste. The study revealed that it has enhanced 20–30 times pollution load of BOD, COD, TDS, TSS, sulphate, chloride, sodium, nitrate, potassium, lignin and phenol after mixing of pulp paper mill waste with river water in all season. Further, it induced the bacterial growth by increasing most probable number value of E. coli was 1.57 × 104, 1.6 × 104, 1.37 × 104and SPC count was 1.68 × 104, 1.64 × 104, 1.67 × 104/100 ml during summer, monsoon, winter respectively. While the most probable number value in river water before mixing of pulp paper mill waste was 1.4 × 102, 1.82 × 102, 1.5 × 102and SPC count was 2.8 × 103, 2.89 × 103, 2.78 × 103/100 ml during summer, monsoon and winter respectively. This indicated from 88 –114 fold increase in most probable number value of E. coli and 56.55 –60.0 times increase in SPC count of river water after mixing of effluent in...

Research paper thumbnail of Isolation and Characterization of Bacterial Strain for the Degradation of Phenol

Research paper thumbnail of Isolation and characterization of two potential pentachlorophenol degrading aerobic bacteria from pulp paper effluent sludge

The Journal of general and …, 2006

Chlorophenols (CPs) are major environmental pollutants discharged from pulp and paper mills, tann... more Chlorophenols (CPs) are major environmental pollutants discharged from pulp and paper mills, tanneries, distilleries, dye and paint manufacturing and pharmaceuticals industry (Crosby, 1981). The major source of CPs is as by-products when chlorine is used for bleaching of pulp and for disinfections of drinking water. Therefore, large amounts of chlorinated aromatic compounds, including CPs, have been discharged into the environment (Steinle et al., 1998). Among chlorophenols, pentachlorophenol (PCP) is a wide spectrum biocide with applications in agriculture, industry and public health (Bevenue and Beckman, 1967). PCP a highly toxic pesticide has been widely used in disinfectants and preservatives (Rao, 1978). The U.S. Environmental Protection Agency regulates PCP as a priority pollutant and also considers 1.0 mg/L of PCP hazardous for land disposal (Freeman, 1989; Sittig, 1981).