Shakila Jannat - Academia.edu (original) (raw)

Papers by Shakila Jannat

BIRDEM Medical Journal, May 15, 2023

Background: Among various immunological markers of colorectal carcinoma, the human epidermal grow... more Background: Among various immunological markers of colorectal carcinoma, the human epidermal growth factor receptor 2 (HER2/neu) is a significant one. Overexpression of HER2/neu in breast cancer has already been established as a poor prognostic marker and trastuzumab (a monoclonal antibody against HER2/neu) has been shown to increase the patient survival. The success of anti-HER2 (Herceptin) therapy in breast cancer has led to the evaluation of HER2/neu expression in colorectal and other cancers. The aim of the study was to determine the expression of HER2/neu in colorectal carcinoma. Methods: This cross-sectional study included 97 diagnosed cases of colorectal carcinoma and was conducted in the Department of Pathology, BIRDEM General Hospital, Dhaka from September 2018 to August 2020. Sections were taken from paraffin blocks for routine hematoxylin and eosin (H&E) stain and immunohistochemical stain with HER2/neu antibody. Relevant clinical and microscopic data including age, sex, tumor site and tumor size were collected and recorded. Results: HER2/neu expression was observed in 47.4% (46) cases in this study. Among which 28.9% cases were found strongly positive (Score 3+) and 18.6% cases were found weakly positive (Score 2+). The mean age of colorectal carcinoma patient was 50.54±14.32 years and the highest expression of HER2/neu was found in 21-30 years age group. The tumor was mostly (66.0%) located in the colon whereas HER2/neu expression was more in rectum (54.5%). The tumor size was t5 cm in 64.9% cases while positive expression of HER2/neu was more in tumors <5 cm in size (50.0%). No statistically significant correlation of HER2/neu expression was found with age, sex, tumor site and size. Conclusion: Expression of HER2/neu has been observed in nearly half of colorectal carcinoma patients with strongly positive in almost 29% cases.

Annals of International medical and Dental Research, Jun 1, 2023

But, this cancer-related mortality remains very high in developing countries because of late diag... more But, this cancer-related mortality remains very high in developing countries because of late diagnosis and delay in treatment. [1] Management of breast carcinoma depends mainly on the expression of some molecular markers like ER, PR, and HER-2/neu. These have been proven to have a great role in the

KYAMC Journal, Jun 5, 2022

Background: Breast carcinoma is a heterogenous disease that represent a major health problem in B... more Background: Breast carcinoma is a heterogenous disease that represent a major health problem in Bangladeshi population. Several molecular markers have been detected over the past several decades, that are important in clinical aspects of breast carcinoma. Most recently, Vascular Endothelial Growth Factor (VEGF) is emerging as a prognostic marker in patients with several types of carcinoma including breast carcinoma. Many anti-angiogenic therapies targeting VEGF has been proven effective in many carcinomas. Objectives:This study was aimed to determine the expression of VEGF in different histomorphological types of breast carcinoma in an attempt to clarify its potential clinical outcome.

KYAMC Journal

A 34-years-old married middle-class housewife without diabetes, hypertension or betel-nut- chewin... more A 34-years-old married middle-class housewife without diabetes, hypertension or betel-nut- chewing hailing from Manikganj, Dhaka; came with the outside diagnosis as neuroendocrine tumor of the gallbladder to the Oncology Department of Khwaja Yunus Ali Medical College and Hospital (KYAMCH). She was admitted in the hospital with complaints of upper abdominal pain, abdominal bloating, weakness, anorexia, nausea and heartburn while taking fatty food. According to her Ultrasonography (USG) of the abdomen, a soft tissue mass was found in the gallbladder with unremarkable routine blood examination including tumor markers. She underwent open cholecystectomy with the suspicion of cancer. Grossly, gallbladder was measures 7.0x 5.0 cm with 0.3 cm wall thickness and on opening a polypoid nodule is found. Before treatment on request from the Oncology Department of KYAMCH, all slides were reviewed and a histopathological diagnosis of paraganglioma was made on the basis of organoid, nested or lobu...

Annals of International Medical and Dental Research

Background: The management of breast carcinoma depends on several molecular markers and tumor sta... more Background: The management of breast carcinoma depends on several molecular markers and tumor stages. In the last decades, estrogen receptors (ER), progesterone receptors (PR), and HER-2/neu have shown good therapeutic responses. Among other molecular markers, vascular endothelial growth factor (VEGF) is becoming more widely used as a prognostic indicator in patients with breast carcinoma. Anti-VEGF therapy already has been proven as an effective chemotherapeutic agent in some other carcinomas. The study aimed to find out the immunohistochemical expression of Vascular Endothelial Growth Factor (VEGF) in breast carcinoma and its possible correlation with the expression of ER, PR, and HER-2/neu and molecular subtypes to evaluate its prognostic value. Material & Methods: This study was conducted in the Department of Pathology, BIRDEM General Hospital, Dhaka, from March 2018 to January 2020. In this study, 45 diagnosed cases of breast carcinoma were enrolled. Slides of all cases were st...

BIRDEM Medical Journal

Background: Among various immunological markers of colorectal carcinoma, the human epidermal grow... more Background: Among various immunological markers of colorectal carcinoma, the human epidermal growth factor receptor 2 (HER2/neu) is a significant one. Overexpression of HER2/neu in breast cancer has already been established as a poor prognostic marker and trastuzumab (a monoclonal antibody against HER2/neu) has been shown to increase the patient survival. The success of anti-HER2 (Herceptin) therapy in breast cancer has led to the evaluation of HER2/neu expression in colorectal and other cancers. The aim of the study was to determine the expression of HER2/neu in colorectal carcinoma. Methods: This cross-sectional study included 97 diagnosed cases of colorectal carcinoma and was conducted in the Department of Pathology, BIRDEM General Hospital, Dhaka from September 2018 to August 2020. Sections were taken from paraffin blocks for routine hematoxylin and eosin (H&E) stain and immunohistochemical stain with HER2/neu antibody. Relevant clinical and microscopic data including age, sex, ...

KYAMC Journal, Mar 30, 2023

KYAMC Journal

There are many well-known causes of acute or sub-acute intestinal obstruction. But Pneumatosis cy... more There are many well-known causes of acute or sub-acute intestinal obstruction. But Pneumatosis cystoides intestinalis (PCI) is a rare cause and can easily be misdiagnosed. PCI refers to presence of air within the wall of small or large intestine. This benign disease having both idiopathic and secondary causes with different contributing pathogenic factors that confuses many doctors. In most of the time it is asymptomatic, but clinical signs and imaging impressions may mimic true abdominal visceral perforation or soft tissue stranding leads to mechanical obstruction. PCI is sometimes incidentally found during radiological evaluation, endoscopic procedure or laparotomy. Surgery is not recommended for PCI if there was no intestinal obstruction, because of high operative risk. So correct diagnosis is imperative as treatment of PCI is generally conservative. We are presenting a case of intestinal obstruction who underwent to laparotomy followed by the histopathological diagnosis as a cas...

KYAMC Journal

Background: The diagnosis of Hirschsprung’s disease (HD) is dependent on the histological study o... more Background: The diagnosis of Hirschsprung’s disease (HD) is dependent on the histological study of rectal ganglion cells. Open rectal biopsy is the mainstay that requires general anesthesia (GA) and carries risk of many complications. Rectal punch biopsy has gained wide acceptance and has become the choice as it is simple, safe, having no need of anesthesia, done bedside with a simple biopsy forcep with high degree of accuracy and virtual absence of any complications. Objective: To find out the efficacy of rectal punch biopsy in the diagnosis of Hirschsprung’s disease. Materials and Methods: A cross sectional descriptive observational study was carried out with the histological findings of 60 rectal punch biopsies with corresponding 60 full thickness rectal biopsies from 60 suspected HD patients from March 2018 to February 2020. Sections made from the paraffin blocks were stained with H&E and were examined for the presence or absence of ganglion cells and hypertrophic nerve fibers i...

KYAMC Journal

Background: Neonatal sepsis is defined as a clinical condition of bacteremia with clinical featur... more Background: Neonatal sepsis is defined as a clinical condition of bacteremia with clinical features of infection in the first 28 days of life. Neonatal sepsis is an important cause of neonatal deaths worldwide. If detected early and treated aggressively and appropriately with antibiotics with good supportive care, it can be possible to save a life. Objective: The present study is carried out to overview the clinical and investigations profile, and outcome of neonatal sepsis admitted in a neonatal intensive care unit (NICU). Materials and Methods: This is a retrospective descriptive study. This study included newborns diagnosed with neonatal sepsis admitted to the Neonatal Intensive Care Unit (NICU) of Khwaja Yunus Ali Medical College and Hospital, Sirajgonj from October 2020 to January 2022. The case records of 50 neonatal sepsis. The case records of these newborns were thoroughly studied and recorded for relevant information including detailed history including maternal, clinical e...

KYAMC Journal

Background: Breast carcinoma is a heterogenous disease that represent a major health problem in B... more Background: Breast carcinoma is a heterogenous disease that represent a major health problem in Bangladeshi population. Several molecular markers have been detected over the past several decades, that are important in clinical aspects of breast carcinoma. Most recently, Vascular Endothelial Growth Factor (VEGF) is emerging as a prognostic marker in patients with several types of carcinoma including breast carcinoma. Many anti-angiogenic therapies targeting VEGF has been proven effective in many carcinomas. Objectives:This study was aimed to determine the expression of VEGF in different histomorphological types of breast carcinoma in an attempt to clarify its potential clinical outcome. Materials and Methods: This study was conducted in the Department of Pathology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) General Hospital, Dhaka from March 2018 to January 2020. In this study, 45 diagnosed cases of breast carcinoma wer...

BIRDEM Medical Journal, May 15, 2023

Background: Among various immunological markers of colorectal carcinoma, the human epidermal grow... more Background: Among various immunological markers of colorectal carcinoma, the human epidermal growth factor receptor 2 (HER2/neu) is a significant one. Overexpression of HER2/neu in breast cancer has already been established as a poor prognostic marker and trastuzumab (a monoclonal antibody against HER2/neu) has been shown to increase the patient survival. The success of anti-HER2 (Herceptin) therapy in breast cancer has led to the evaluation of HER2/neu expression in colorectal and other cancers. The aim of the study was to determine the expression of HER2/neu in colorectal carcinoma. Methods: This cross-sectional study included 97 diagnosed cases of colorectal carcinoma and was conducted in the Department of Pathology, BIRDEM General Hospital, Dhaka from September 2018 to August 2020. Sections were taken from paraffin blocks for routine hematoxylin and eosin (H&E) stain and immunohistochemical stain with HER2/neu antibody. Relevant clinical and microscopic data including age, sex, tumor site and tumor size were collected and recorded. Results: HER2/neu expression was observed in 47.4% (46) cases in this study. Among which 28.9% cases were found strongly positive (Score 3+) and 18.6% cases were found weakly positive (Score 2+). The mean age of colorectal carcinoma patient was 50.54±14.32 years and the highest expression of HER2/neu was found in 21-30 years age group. The tumor was mostly (66.0%) located in the colon whereas HER2/neu expression was more in rectum (54.5%). The tumor size was t5 cm in 64.9% cases while positive expression of HER2/neu was more in tumors <5 cm in size (50.0%). No statistically significant correlation of HER2/neu expression was found with age, sex, tumor site and size. Conclusion: Expression of HER2/neu has been observed in nearly half of colorectal carcinoma patients with strongly positive in almost 29% cases.

Annals of International medical and Dental Research, Jun 1, 2023

But, this cancer-related mortality remains very high in developing countries because of late diag... more But, this cancer-related mortality remains very high in developing countries because of late diagnosis and delay in treatment. [1] Management of breast carcinoma depends mainly on the expression of some molecular markers like ER, PR, and HER-2/neu. These have been proven to have a great role in the

KYAMC Journal, Jun 5, 2022

Background: Breast carcinoma is a heterogenous disease that represent a major health problem in B... more Background: Breast carcinoma is a heterogenous disease that represent a major health problem in Bangladeshi population. Several molecular markers have been detected over the past several decades, that are important in clinical aspects of breast carcinoma. Most recently, Vascular Endothelial Growth Factor (VEGF) is emerging as a prognostic marker in patients with several types of carcinoma including breast carcinoma. Many anti-angiogenic therapies targeting VEGF has been proven effective in many carcinomas. Objectives:This study was aimed to determine the expression of VEGF in different histomorphological types of breast carcinoma in an attempt to clarify its potential clinical outcome.

KYAMC Journal

A 34-years-old married middle-class housewife without diabetes, hypertension or betel-nut- chewin... more A 34-years-old married middle-class housewife without diabetes, hypertension or betel-nut- chewing hailing from Manikganj, Dhaka; came with the outside diagnosis as neuroendocrine tumor of the gallbladder to the Oncology Department of Khwaja Yunus Ali Medical College and Hospital (KYAMCH). She was admitted in the hospital with complaints of upper abdominal pain, abdominal bloating, weakness, anorexia, nausea and heartburn while taking fatty food. According to her Ultrasonography (USG) of the abdomen, a soft tissue mass was found in the gallbladder with unremarkable routine blood examination including tumor markers. She underwent open cholecystectomy with the suspicion of cancer. Grossly, gallbladder was measures 7.0x 5.0 cm with 0.3 cm wall thickness and on opening a polypoid nodule is found. Before treatment on request from the Oncology Department of KYAMCH, all slides were reviewed and a histopathological diagnosis of paraganglioma was made on the basis of organoid, nested or lobu...

Annals of International Medical and Dental Research

Background: The management of breast carcinoma depends on several molecular markers and tumor sta... more Background: The management of breast carcinoma depends on several molecular markers and tumor stages. In the last decades, estrogen receptors (ER), progesterone receptors (PR), and HER-2/neu have shown good therapeutic responses. Among other molecular markers, vascular endothelial growth factor (VEGF) is becoming more widely used as a prognostic indicator in patients with breast carcinoma. Anti-VEGF therapy already has been proven as an effective chemotherapeutic agent in some other carcinomas. The study aimed to find out the immunohistochemical expression of Vascular Endothelial Growth Factor (VEGF) in breast carcinoma and its possible correlation with the expression of ER, PR, and HER-2/neu and molecular subtypes to evaluate its prognostic value. Material & Methods: This study was conducted in the Department of Pathology, BIRDEM General Hospital, Dhaka, from March 2018 to January 2020. In this study, 45 diagnosed cases of breast carcinoma were enrolled. Slides of all cases were st...

BIRDEM Medical Journal

Background: Among various immunological markers of colorectal carcinoma, the human epidermal grow... more Background: Among various immunological markers of colorectal carcinoma, the human epidermal growth factor receptor 2 (HER2/neu) is a significant one. Overexpression of HER2/neu in breast cancer has already been established as a poor prognostic marker and trastuzumab (a monoclonal antibody against HER2/neu) has been shown to increase the patient survival. The success of anti-HER2 (Herceptin) therapy in breast cancer has led to the evaluation of HER2/neu expression in colorectal and other cancers. The aim of the study was to determine the expression of HER2/neu in colorectal carcinoma. Methods: This cross-sectional study included 97 diagnosed cases of colorectal carcinoma and was conducted in the Department of Pathology, BIRDEM General Hospital, Dhaka from September 2018 to August 2020. Sections were taken from paraffin blocks for routine hematoxylin and eosin (H&E) stain and immunohistochemical stain with HER2/neu antibody. Relevant clinical and microscopic data including age, sex, ...

KYAMC Journal, Mar 30, 2023

KYAMC Journal

There are many well-known causes of acute or sub-acute intestinal obstruction. But Pneumatosis cy... more There are many well-known causes of acute or sub-acute intestinal obstruction. But Pneumatosis cystoides intestinalis (PCI) is a rare cause and can easily be misdiagnosed. PCI refers to presence of air within the wall of small or large intestine. This benign disease having both idiopathic and secondary causes with different contributing pathogenic factors that confuses many doctors. In most of the time it is asymptomatic, but clinical signs and imaging impressions may mimic true abdominal visceral perforation or soft tissue stranding leads to mechanical obstruction. PCI is sometimes incidentally found during radiological evaluation, endoscopic procedure or laparotomy. Surgery is not recommended for PCI if there was no intestinal obstruction, because of high operative risk. So correct diagnosis is imperative as treatment of PCI is generally conservative. We are presenting a case of intestinal obstruction who underwent to laparotomy followed by the histopathological diagnosis as a cas...

KYAMC Journal

Background: The diagnosis of Hirschsprung’s disease (HD) is dependent on the histological study o... more Background: The diagnosis of Hirschsprung’s disease (HD) is dependent on the histological study of rectal ganglion cells. Open rectal biopsy is the mainstay that requires general anesthesia (GA) and carries risk of many complications. Rectal punch biopsy has gained wide acceptance and has become the choice as it is simple, safe, having no need of anesthesia, done bedside with a simple biopsy forcep with high degree of accuracy and virtual absence of any complications. Objective: To find out the efficacy of rectal punch biopsy in the diagnosis of Hirschsprung’s disease. Materials and Methods: A cross sectional descriptive observational study was carried out with the histological findings of 60 rectal punch biopsies with corresponding 60 full thickness rectal biopsies from 60 suspected HD patients from March 2018 to February 2020. Sections made from the paraffin blocks were stained with H&E and were examined for the presence or absence of ganglion cells and hypertrophic nerve fibers i...

KYAMC Journal

Background: Neonatal sepsis is defined as a clinical condition of bacteremia with clinical featur... more Background: Neonatal sepsis is defined as a clinical condition of bacteremia with clinical features of infection in the first 28 days of life. Neonatal sepsis is an important cause of neonatal deaths worldwide. If detected early and treated aggressively and appropriately with antibiotics with good supportive care, it can be possible to save a life. Objective: The present study is carried out to overview the clinical and investigations profile, and outcome of neonatal sepsis admitted in a neonatal intensive care unit (NICU). Materials and Methods: This is a retrospective descriptive study. This study included newborns diagnosed with neonatal sepsis admitted to the Neonatal Intensive Care Unit (NICU) of Khwaja Yunus Ali Medical College and Hospital, Sirajgonj from October 2020 to January 2022. The case records of 50 neonatal sepsis. The case records of these newborns were thoroughly studied and recorded for relevant information including detailed history including maternal, clinical e...

KYAMC Journal

Background: Breast carcinoma is a heterogenous disease that represent a major health problem in B... more Background: Breast carcinoma is a heterogenous disease that represent a major health problem in Bangladeshi population. Several molecular markers have been detected over the past several decades, that are important in clinical aspects of breast carcinoma. Most recently, Vascular Endothelial Growth Factor (VEGF) is emerging as a prognostic marker in patients with several types of carcinoma including breast carcinoma. Many anti-angiogenic therapies targeting VEGF has been proven effective in many carcinomas. Objectives:This study was aimed to determine the expression of VEGF in different histomorphological types of breast carcinoma in an attempt to clarify its potential clinical outcome. Materials and Methods: This study was conducted in the Department of Pathology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) General Hospital, Dhaka from March 2018 to January 2020. In this study, 45 diagnosed cases of breast carcinoma wer...