Sharon Angela Tanuseputero - Academia.edu (original) (raw)

Papers by Sharon Angela Tanuseputero

Research paper thumbnail of Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model

International Journal of Molecular Sciences

Fermentation is thought to alter the composition and bioavailability of bioactive compounds in ri... more Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran. However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated. This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function. The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions. The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity. The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells. Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells ...

Research paper thumbnail of Utilization of rambutan (nephelium lappaceum l.) peel and its extract to inhibit alcoholic fermentation of sugar palm (arenga pinnata merr.) Sap for sugar production

Rambutan (Nephelium lappaceum L.) peels had been studied for its inhibitory effects toward alcoho... more Rambutan (Nephelium lappaceum L.) peels had been studied for its inhibitory effects toward alcoholic fermentation in apple cider and cassava tapai. The aim of this research was to utilize rambutan peel and its extract to inhibit the fermentation occurring in palm sap. Preliminary research was conducted to investigate the suitable concentration of rambutan peels to give effective inhibition of fermentation. Based on the result, dried rambutan peels were ineffective, whereas size-reduced peels were able to inhibit fermentation and further investigated by other fellow researchers. In the main research stage I semi-dried peels (moisture content~40%) with concentrations 45, 50 and 55% were applied to palm sap. In addition, extracts of rambutan peel derived of the maceration of peels using ethyl acetic extract were also used with concentrations of 2, 4 and 6%. Samples were analyzed for pH, total titratable acidity, total reducing sugar, alcohol content, total dissolved solids, and microbi...

Research paper thumbnail of Intravenous Arginine Administration Benefits CD4+ T-Cell Homeostasis and Attenuates Liver Inflammation in Mice with Polymicrobial Sepsis

Nutrients, 2020

This study investigated the effects of a single dose of arginine (Arg) administration at the begi... more This study investigated the effects of a single dose of arginine (Arg) administration at the beginning of sepsis on CD4+ T-cell regulation and liver inflammation in C57BL/6J mice. Mice were divided into normal control (NC), sham (SH), sepsis saline (SS), and sepsis Arg (SA) groups. An inducible nitric oxide (NO) synthase (iNOS) inhibitor was administered to additional sepsis groups to evaluate the role of NO during sepsis. Sepsis was induced using cecal ligation and puncture (CLP). The SS and SA groups received saline or Arg (300 mg/kg body weight) via tail vein 1 h after CLP. Mice were euthanized at 12 and 24 h post-CLP. Blood, para-aortic lymph nodes, and liver tissues were collected for further measurement. The findings showed that sepsis resulted in decreases in blood and para-aortic lymph node CD4+ T-cell percentages, whereas percentages of interleukin (IL)-4- and IL-17-expressing CD4+ T cells were upregulated. Compared to the SS group, Arg administration resulted in maintained...

Research paper thumbnail of Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis

Mediators of Inflammation

Acute kidney injury (AKI) is a major complication of sepsis. Nucleotide-binding domain-like recep... more Acute kidney injury (AKI) is a major complication of sepsis. Nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasomes are multiprotein complexes that mediate septic AKI. L-arginine (Arg) is a conditionally essential amino acid in catabolic conditions and a substrate for nitric oxide (NO) production; however, its use in sepsis is controversial. This study investigated the effect of intravenous Arg supplementation on modulating NLRP3 inflammasome activity in relation to septic AKI. Mice were divided into normal control (NC), sham, sepsis saline (SS), and sepsis Arg (SA) groups. In order to investigate the role of NO, L-N6-(1-iminoethyl)-lysine hydrochloride (L-NIL), an inducible NO synthase inhibitor, was administered to the sepsis groups. Sepsis was induced using cecal ligation and puncture (CLP). The SS and SA groups received saline or Arg via tail vein 1 h after CLP. Mice were sacrificed at 6, 12, and 24 h after sepsis. The results showed that compared to the NC grou...

Research paper thumbnail of Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model

International Journal of Molecular Sciences

Fermentation is thought to alter the composition and bioavailability of bioactive compounds in ri... more Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran. However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated. This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function. The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions. The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity. The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells. Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells ...

Research paper thumbnail of Utilization of rambutan (nephelium lappaceum l.) peel and its extract to inhibit alcoholic fermentation of sugar palm (arenga pinnata merr.) Sap for sugar production

Rambutan (Nephelium lappaceum L.) peels had been studied for its inhibitory effects toward alcoho... more Rambutan (Nephelium lappaceum L.) peels had been studied for its inhibitory effects toward alcoholic fermentation in apple cider and cassava tapai. The aim of this research was to utilize rambutan peel and its extract to inhibit the fermentation occurring in palm sap. Preliminary research was conducted to investigate the suitable concentration of rambutan peels to give effective inhibition of fermentation. Based on the result, dried rambutan peels were ineffective, whereas size-reduced peels were able to inhibit fermentation and further investigated by other fellow researchers. In the main research stage I semi-dried peels (moisture content~40%) with concentrations 45, 50 and 55% were applied to palm sap. In addition, extracts of rambutan peel derived of the maceration of peels using ethyl acetic extract were also used with concentrations of 2, 4 and 6%. Samples were analyzed for pH, total titratable acidity, total reducing sugar, alcohol content, total dissolved solids, and microbi...

Research paper thumbnail of Intravenous Arginine Administration Benefits CD4+ T-Cell Homeostasis and Attenuates Liver Inflammation in Mice with Polymicrobial Sepsis

Nutrients, 2020

This study investigated the effects of a single dose of arginine (Arg) administration at the begi... more This study investigated the effects of a single dose of arginine (Arg) administration at the beginning of sepsis on CD4+ T-cell regulation and liver inflammation in C57BL/6J mice. Mice were divided into normal control (NC), sham (SH), sepsis saline (SS), and sepsis Arg (SA) groups. An inducible nitric oxide (NO) synthase (iNOS) inhibitor was administered to additional sepsis groups to evaluate the role of NO during sepsis. Sepsis was induced using cecal ligation and puncture (CLP). The SS and SA groups received saline or Arg (300 mg/kg body weight) via tail vein 1 h after CLP. Mice were euthanized at 12 and 24 h post-CLP. Blood, para-aortic lymph nodes, and liver tissues were collected for further measurement. The findings showed that sepsis resulted in decreases in blood and para-aortic lymph node CD4+ T-cell percentages, whereas percentages of interleukin (IL)-4- and IL-17-expressing CD4+ T cells were upregulated. Compared to the SS group, Arg administration resulted in maintained...

Research paper thumbnail of Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis

Mediators of Inflammation

Acute kidney injury (AKI) is a major complication of sepsis. Nucleotide-binding domain-like recep... more Acute kidney injury (AKI) is a major complication of sepsis. Nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasomes are multiprotein complexes that mediate septic AKI. L-arginine (Arg) is a conditionally essential amino acid in catabolic conditions and a substrate for nitric oxide (NO) production; however, its use in sepsis is controversial. This study investigated the effect of intravenous Arg supplementation on modulating NLRP3 inflammasome activity in relation to septic AKI. Mice were divided into normal control (NC), sham, sepsis saline (SS), and sepsis Arg (SA) groups. In order to investigate the role of NO, L-N6-(1-iminoethyl)-lysine hydrochloride (L-NIL), an inducible NO synthase inhibitor, was administered to the sepsis groups. Sepsis was induced using cecal ligation and puncture (CLP). The SS and SA groups received saline or Arg via tail vein 1 h after CLP. Mice were sacrificed at 6, 12, and 24 h after sepsis. The results showed that compared to the NC grou...