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Papers by Sheila Johnston

Journal of Bacteriology, 1993

A transformation scheme for Cryptococcus neoformans to yield high-frequency, integrative events w... more A transformation scheme for Cryptococcus neoformans to yield high-frequency, integrative events was developed. Adenine auxotrophs from a clinical isolate of C. neoformans serotype A were complemented by the cryptococcal phosphoribosylaminoimidazole carboxylase gene (ade2) with a biolistic DNA delivery system. Comparison of two DNA delivery systems (electroporation versus a biolistic system) showed notable differences. The biolistic system did not require linear vectors and transformed each auxotrophic strain at similar frequencies. Examination of randomly selected transformants by biolistics showed that 15 to 40% were stable, depending on the recipient auxotroph, with integrative events identified in all stable transformants by DNA analysis. Although the ade2 cDNA copy transformed at a low frequency, DNA analysis found homologous recombination in each of these transformants. DNA analysis of stable transformants receiving genomic ade2 revealed ectopic integration in a majority of cas...

physica status solidi (RRL) - Rapid Research Letters, 2012

Screen, 1982

CINEMA, TELEVISION: TWO different media, each with its own separate apparatus of production and d... more CINEMA, TELEVISION: TWO different media, each with its own separate apparatus of production and dissemination. In Britain this is taken for granted-but the West German model suggests that it heed not be so. It is by now almost a commonplace that the New German Cinema owes ...

Journal of Vision, 2010

The capacity of visual short-term memory can be increased significantly when the to-be-remembered... more The capacity of visual short-term memory can be increased significantly when the to-be-remembered objects are presented sequentially across two displays, rather than simultaneously in one display (Ihssen, Linden, & Shapiro, VSS, 2009). Interestingly, a similar performance increase is ...

Cognitive Therapy and Research, 1997

This study examined the effects of thought suppression, prior to an experimental pain procedure, ... more This study examined the effects of thought suppression, prior to an experimental pain procedure, on subsequent pain experience. The study also examined the role of thought intrusions in mediating the relation between (1) thought suppression and pain, and (2) catastrophizing and pain. Catastrophizers (n = 34) and noncatastrophizers (n = 36) made pain ratings while one arm was immersed in

British Journal of Sports Medicine, 2009

The purpose of this study was to examine ratings of perceived exertion (RPE) and performance duri... more The purpose of this study was to examine ratings of perceived exertion (RPE) and performance during repetitive maximal effort 40 km time trials as well as after an intervention that aimed to decrease certainty about the remaining distance of the exercise bout. In addition, we examined the RPE during exercise bouts of markedly different duration. Methods: Part 1: 12 well-trained, competitive-level cyclists completed five 40 km time trials. During the final time trial all feedback was withheld until the final kilometre. In addition, to cause confusion about the remaining distance, they were asked to report their RPE at random intervals from 18 km to 38 km. Part 2: 6 welltrained, recreation-level cyclists randomly completed a 5 km, 10 km, 40 km and 100 km time trial. Results: Part 1: Mean RPE increased during the first four trials and decreased during the final trial. The rate of RPE progression increased in linearity during the first four trials and became more conservative in the final trial. These changes were directly related to performance. Part 2: Mean RPE for longer duration trials (40 km, 100 km) were lower during the first half of trial duration but matched those of shorter trials in the final 20%. Conclusions: Increased familiarity of the exercise bout and certainty about its endpoint are associated with a more aggressive RPE strategy that produces a superior exercise performance. Certainty about the endpoint and the duration of exercise affect both the RPE strategy and performance.

American Journal of Respiratory and Critical Care Medicine, 2006

Background: Respiratory syncytial virus (RSV) is increasingly recognized as an important pathogen... more Background: Respiratory syncytial virus (RSV) is increasingly recognized as an important pathogen in adults with cardiopulmonary disease. It has been associated with acute exacerbations of chronic obstructive pulmonary disease (COPD); however, it has also been detected in the lower airway in the stable state, but the consequences of RSV in stable disease have not previously been determined. We therefore studied the consequences of RSV persistence in adults with COPD and its effect on airway inflammation and lung function decline. Methods: A total of 241 sputum samples from 74 patients with COPD (FEV 1 % predicted, 39.2%; interquartile range, 29.6-57.8%) were collected quarterly in the stable state over 2 yr. RSV was detected by polymerase chain reaction (PCR), quantitative microbiology was performed, and inflammatory cytokines were quantified by ELISA. Results: RSV RNA was detected in 32.8% of sputum samples. Patients in whom RSV was more frequently detected (Ͼ 50% of samples RSV PCR-positive, n ϭ 18) had higher airway inflammation and faster FEV 1 decline over the study (101.4 ml/yr [95% confidence interval, 57.1-145.8]) compared with those with less frequent detection of RSV (n ϭ 56; 51.2 ml/yr [31.7-70.8]; p ϭ 0.01). The observed relationship between RSV detection and accelerated lung function decline was independent of smoking status, exacerbation frequency, and lower airway bacterial load. Conclusions: Persistent RSV detection in patients with COPD is associated with airway inflammation and accelerated decline in FEV 1. Chronic RSV infection may be a novel therapeutic target to alter the natural history of COPD.

Journal of Clinical Nursing, 2012

This study explored decision-making experiences of patients with stage 5 chronic kidney disease w... more This study explored decision-making experiences of patients with stage 5 chronic kidney disease when opting for conservative management of their renal failure. Dialysis is an invasive treatment, and for some older patients, there is an associated treatment burden of dialysis-related symptoms. An alternative choice is conservative management, but little is known about those who make this decision and how they are supported through the process. Qualitative practitioner research study. Data were generated from nine patients&amp;amp;amp;amp;amp;#39; naturally occurring clinic consultations with a renal clinical nurse specialist between May 2010 - July 2010. Interviews were transcribed verbatim and findings fed back at three multi-disciplinary meetings to check for relevance and resonance. Common themes were identified and codes applied. Patients reported age and having to travel three times a week to hospital for dialysis as reasons not to opt for treatment. Others felt well without dialysis not wanting to upset the &amp;amp;amp;amp;amp;#39;status quo&amp;amp;amp;amp;amp;#39; or to burden loved ones. Most felt equipped to make the decision following explanation and discussion with the clinical nurse specialist in the renal clinic. Patients opting for conservative management give numerous reasons for this including old age, travel limitations, feeling well without dialysis and not wanting to be a burden, but appear content with their decision. One-to-one discussions with the clinical nurse specialist appear helpful during the decision-making process presenting an opportunity for advancing nursing roles in the chronic kidney disease service. Understanding patients&amp;amp;amp;amp;amp;#39; reasons for refusing dialysis assists in supporting until death. There is an opportunity for developing nursing practice to meet the multi-faceted needs of this group.

This paper discusses the need for employer internship opportunities that are based on employer-co... more This paper discusses the need for employer internship opportunities that are based on employer-community partnerships. Interpreter graduates lack the experience necessary to allow them to successfully make the transition between interpreting student and working community interpreter. The failure to make this transition impedes the professionalisation of the field as interpreters with widely varying skills are providing service in the community. The Canadian Hearing Society (CHS), an organization serving Deaf, deafened and hard of hearing consumers, has developed and piloted an Interpreter Internship Program. Unlike a placement opportunity, interns are graduates from interpreter training programs hired by the agency. The process of establishing such a program, developing curriculum and blending formal training with hands on experience is highlighted. Discussion will focus on initial outcomes and lessons learned.

Sight and Sound, 1999

... Biographies and memoirs. Autores: Stella Bruzzi; Localización: Sight & sound, ISSN 0037-4... more ... Biographies and memoirs. Autores: Stella Bruzzi; Localización: Sight & sound, ISSN 0037-4806, Vol. 9, Nº 2, 1999 , pág. 32. Fundación Dialnet. Acceso de usuarios registrados. Acceso de usuarios registrados Usuario. Contraseña. Entrar. Mi Dialnet. ...

This biography details the background and rise to fame of Keanu Reeves, from his birth in Beirut ... more This biography details the background and rise to fame of Keanu Reeves, from his birth in Beirut and upbringing in Toronto to his residency in Los Angeles. The book examines his relationship with friends and co-stars, including the late River Phoenix.

Index on Censorship, 1995

... Cameron co-wrote the screenplay for Strange Days. But she still believes it possible to make ... more ... Cameron co-wrote the screenplay for Strange Days. But she still believes it possible to make 'captivating' crowd-pleasers while maintaining a critical distance. ... In Strange Days the SQUID clips are filtered through the character of Lenny Nero. ...

Healthcare

Chronic kidney disease (CKD) stages 3-5 now affects 8.5% of adults in the United Kingdom; with 4%... more Chronic kidney disease (CKD) stages 3-5 now affects 8.5% of adults in the United Kingdom; with 4% of patients expected to reach stage 5 CKD. Increasing numbers of older patients are contributing to the growth of demand of kidney services. With the exception of transplantation, dialysis has been the main form of renal replacement therapy (RRT) for advanced CKD. This elderly population is usually too frail and has many other co-existing medical complaints or co morbidities to undergo transplantation. Dialysis is an invasive treatment, and some frail elderly patients can experience many dialysis related symptoms. An alternative option for these patients is to choose conservative management (CM) of their stage 5 CKD. These patients often have complex supportive and palliative care needs. The frequency, severity and distress caused by symptoms related to stage 5 CKD are often under recognized and under treated. There is a need for early identification and management of symptoms as they present in patients with stage 5 CKD being managed conservatively. Symptom assessment should be focused on anticipating, identifying and alleviating any symptoms. This needs to be incorporated into the regular practice of those managing CM patients.