Hossain Shekhar - Academia.edu (original) (raw)

Papers by Hossain Shekhar

Research paper thumbnail of Detection of −α 3.7 and _ _SEA deletions in α-globin gene in the Bangladeshi population

Research Square (Research Square), Jan 2, 2024

Research paper thumbnail of Q531L mutation in the capsid protein of hepatitis E virus genotype 1 causes infections in patients with altered immunity and immunosuppressive condition: Mechanism based on wet lab and in-silico findings

Research paper thumbnail of Application of Engineering in Biomedical Science: The Window of Opportunity

Current Trends in Biomedical Engineering & Biosciences, Feb 20, 2017

Research paper thumbnail of Application of Biochemical Tests and Machine Learning Techniques to Diagnose and Evaluate Liver Disease

Advances in Bioscience and Biotechnology, 2021

Background: The liver function tests (LFTs) remain one of the most commonly employed clinical mea... more Background: The liver function tests (LFTs) remain one of the most commonly employed clinical measures for the diagnosis of hepatobiliary disease. LFTs sometimes referred to as hepatic panel help to determine the health of liver, monitor the progression of a disease and measure the severity of a disease particularly scarring or cirrhosis of the liver. Aims: In this study, we present a new approach to evaluate the natural progression of liver disease through the assessment of eight biochemical parameters: serum total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), Alkaline phosphatase (ALP), total protein (TP), albumin (ALB), albumin/globulin (A/G) ratio, and alpha-fetoprotein (AFP) as well as two machine learning (ML) tools-Random Forest and CART to substantive the outcome. Methods: The study was carried out in a total of 100 subjects which included healthy controls (group I-25 patients), patients with acute hepatitis (group II-25 patients), chronic hepatitis (group III-25 patients) and hepatocellular carcinoma (group IV-25 patients) applying both biochemical and Machine Learning methods. Results: Of the eight parameters tested, all except ALP (p = 0.426), showed an overall discriminatory ability as judged by one-factor analysis of variance (p < 0.0001). We also assessed the differences among group means by least significance difference (LSD). The analysis showed that TB remained significantly elevated in groups II, III, and IV as compared to controls (p < 0.05). ALP did not have any discriminatory power among the four groups tested. ALT and AST were good discriminators only between the control groups and groups II and III. TP, ALB, and A/G ratio were decreased significantly in groups III and IV as compared to controls. Group III and IV were almost indistinguishable using these biochemical parameters except for AFP, which was found to be elevated only in group IV.

Research paper thumbnail of Analysis of Proteotranscriptomics Landscape Reveals Differentially Regulated Pathways in &lt;i&gt;Toxoplasma gondii&lt;/i&gt; Infected Mouse Liver

Computational molecular bioscience, 2022

Toxoplasma gondii (T. gondii) an intracellular protozoan parasite, infects mammals including huma... more Toxoplasma gondii (T. gondii) an intracellular protozoan parasite, infects mammals including human population worldwide. Upon primary infection, the parasite contributes to mild flu like symptoms in immune competent host, but life threatening complication is seen in immune compromised patients and in pregnant women. Understanding the host-parasite interaction is critical for understanding the pathogenesis and biology parasite reactivation in the host. In this study, we used proteotrasncriptomics analyses by integrating the transcriptomics and proteomics data of T. gondii infected mouse liver to uncover the effector molecules responsible for disease pathogenesis that can be used as candidate markers for diagnosis and drug target. With this aim, we systematically integrated transcriptomicand proteomic data, representing the parasite infected mouse liver. Out of 2758 differentially expressed genes (DEGs) and 301 differentially expressed proteins (DEPs), 159 overlapping genes were identified. Among them, 86 genes were upregulated and 72 were downregulated in their respective mRNA and protein levels in the infected condition. Gene Ontology (GO) analysis revealed that the upregulated genes were mostly associated with immune system processes whereas the downregulated genes were involved in oxidation-reduction process and metabolism of lipid, and fatty acids. Protein-protein interaction (PPI) network analysis uncovered an interaction-hub including, Psmb8, Psmb9 and Tap1 for upregulated proteins and Cyp1A2, Cyp4A10 and Cyp3A11 for down-regulated proteins. Further studies are needed to validating these effector molecules. These molecules are likely to play a vital role in disease pathogenesis, as well as can be used as potential diagnostic marker and drug target candidates.

Research paper thumbnail of Correlations between well water and human samples for each element

<p>Spearman’s rank correlation coefficient (n = 30) for correlation between drinking well w... more <p>Spearman’s rank correlation coefficient (n = 30) for correlation between drinking well water and human urine, toenail and hair samples in Bangladesh in each element (arsenic, magnesium, calcium, strontium and barium) are shown. * and **, Statistically significant correlation (*, p<0.05; **, p<0.01).</p

Research paper thumbnail of Barium absorption in the indicated percent of weight of MF-HT

<p>After the indicated percentages of weight of MF-HT (percent of MF-HT) had been suspended... more <p>After the indicated percentages of weight of MF-HT (percent of MF-HT) had been suspended in solutions containing 1,000 µg/L barium, the MF-HT-suspended solutions were shaken at 300 rpm for 1 hour. Barium concentrations (means ± SD) in the supernatants after the solutions had been centrifuged are presented.</p

Research paper thumbnail of Exploring the Nutrition and Health Benefits of Functional Foods

This chapter focuses on the potential health benefits of camel milk including angiotension I-conv... more This chapter focuses on the potential health benefits of camel milk including angiotension I-converting enzyme-inhibitory, anti-cancer and antioxidant activities, antidiabetic, antimicrobial and hypoallergenicity effects. The bioactivity of oligosaccharide, conjugated linoleic acid and D-amino acid in camel milk is provided. The proposed mechanisms behind these bioactive components and potential health claims are explained. This chapter also describes camel milk composition, nutritional value, production and population. The current available information in the literature on camel milk is not abundant. More research is needed to give better understanding on functional properties of camel milk.

Research paper thumbnail of Association of genetic variants located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes with type 2 Diabetes Mellitus in Bangladeshi population

Background Genome-Wide Association Studies have identified genetic polymorphisms located at the A... more Background Genome-Wide Association Studies have identified genetic polymorphisms located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes on chromosome 3 as susceptibility loci for type 2 diabetes mellitus (T2DM) in different population. The present study investigates the association of these two susceptibility loci (rs11708067 at the ADCY5 and rs11926707 at the PTH1R gene) with T2DM in Bangladeshi population. Furthermore, the association of circulating levels of vitamin D, Calcium (Ca), phosphate, and parathyroid hormone (PTH) with T2DM and the different genotypes of these SNPs were also studied.Methods and Materials A total of 415 individuals (211 T2DM patients and 204 healthy controls) were enrolled in the current study. The rs11708067 and rs11926707 were genotyped using the Taqman SNP Genotyping Assays. Univariate logistic regression analyses were performed for parameter associations with T2DM.Results In a sex-stratified analysis, the variant a...

Research paper thumbnail of Additional file 7: Figure S6. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Temperature shifted difference curves of unknown carrier parents subjected to HRM analysis by 2nd... more Temperature shifted difference curves of unknown carrier parents subjected to HRM analysis by 2nd set of primers. (TIFF 487 kb)

Research paper thumbnail of Additional file 4: Figure S3. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Normalized melt curve patterns of heterozygous c.27_28insG, c.46delT, c.47Gâ >â A and c.92Gâ &... more Normalized melt curve patterns of heterozygous c.27_28insG, c.46delT, c.47Gâ >â A and c.92Gâ >â C mutations. (TIFF 211 kb)

Research paper thumbnail of Additional file 3: Figure S2. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Normalized melt curve patterns of homozygous and heterozygous c.126_129delCTTT mutations. (TIFF 1... more Normalized melt curve patterns of homozygous and heterozygous c.126_129delCTTT mutations. (TIFF 173 kb)

Research paper thumbnail of Additional file 2: Figure S1. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Normalized melt curve patterns of homozygous and heterozygous c.79G > A and c.92 + 5G > C m... more Normalized melt curve patterns of homozygous and heterozygous c.79G > A and c.92 + 5G > C mutations. (TIFF 232 kb)

Research paper thumbnail of MOESM1 of Nationwide carrier detection and molecular characterization of β-thalassemia and hemoglobin E variants in Bangladeshi population

Additional file 1: Table S1. Hematological features of the participants having high HbA2 level (H... more Additional file 1: Table S1. Hematological features of the participants having high HbA2 level (HbA2 > 3.5%) without mutation in beta globin gene of hemoglobin.

Research paper thumbnail of TYPES OF ROS , SOURCE OF SYNTHESIS AND THE DAMAGE CAUSED BY THE PRODUCTION OF ROS 2 . 1 . Types of Reactive Species

Redox degenerative reactions of the biological system inevitably produce reactive oxygen species ... more Redox degenerative reactions of the biological system inevitably produce reactive oxygen species (ROS) and their derivatives. Oxidative stress is the result of an imbalance in pro-oxidant/antioxidant homeostasis that leads to the generation of toxic reactive oxygen species (ROS), such as hydrogen peroxide, organic hydro peroxides, nitric oxide, superoxide and hydroxyl radicals etc. Information are accumulating steadily, supporting the general importance of oxidative damage of tissue and cellular components as a primary or secondary causative factor in many different human diseases and aging processes. Many of the recent landmarks in scientific research have shown that in human beings, oxidative stress has been implicated in the progression of major health problems by inactivating the metabolic enzymes and damaging important cellular components, oxidizing the nucleic acids, leading to cardiovascular diseases, eye disorders, joint disorders, neurological diseases (Alzheimer’s disease,...

Research paper thumbnail of Molecular Diagnostic Approach Prevails Superior Over Conventional GelElectrophoresis Method in Detecting Sickle Cell Anemia

Journal of Molecular Biomarkers & Diagnosis, 2018

Sickle cell anemia is defined as homozygosity caused by the mutation of the glutamic acid residue... more Sickle cell anemia is defined as homozygosity caused by the mutation of the glutamic acid residue to valine in the β-globin gene. Sickle cell disease is an increasing global health burden with the estimated number of patients increasing in a concerning manner. Here we report a very interesting and clinically insightful case of hemoglobinopathy which was initially suspected to be Hb S/D Punjab-a rare type of hemoglobinopathy as diagnosed by the hemoglobinelectrophoresis technique. Despite the diagnosis report indicating the rare Hb S/D Punjab, the uncharacteristic clinical presentation of the patient which was not coherent with the classical symptoms of Hb S/D Punjab, forced the clinicians to turn their attention to molecular diagnosis. To clarify the etiology of the clinical case, a sequencing-based molecular diagnosis approach was adopted that revealed the mutational signature of sickle cell anemia (SCA). This case can be regarded as a prominent example where the molecular techniques lead to the correct diagnosis matching with the clinical symptoms while the conventional diagnostic approach failed.

Research paper thumbnail of A computational in silico approach to predict high-risk coding and non-coding SNPs of human PLCG1 gene

PLoS ONE, 2021

PLCG1 gene is responsible for many T-cell lymphoma subtypes, including peripheral T-cell lymphoma... more PLCG1 gene is responsible for many T-cell lymphoma subtypes, including peripheral T-cell lymphoma (PTCL), angioimmunoblastic T-cell lymphoma (AITL), cutaneous T-cell lymphoma (CTCL), adult T-cell leukemia/lymphoma along with other diseases. Missense mutations of this gene have already been found in patients of CTCL and AITL. The non-synonymous single nucleotide polymorphisms (nsSNPs) can alter the protein structure as well as its functions. In this study, probable deleterious and disease-related nsSNPs in PLCG1 were identified using SIFT, PROVEAN, PolyPhen-2, PhD-SNP, Pmut, and SNPS&GO tools. Further, their effect on protein stability was checked along with conservation and solvent accessibility analysis by I-mutant 2.0, MUpro, Consurf, and Netsurf 2.0 server. Some SNPs were finalized for structural analysis with PyMol and BIOVIA discovery studio visualizer. Out of the 16 nsSNPs which were found to be deleterious, ten nsSNPs had an effect on protein stability, and six mutations (L41...

Research paper thumbnail of Additional file 1: Table S1. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Mutational status of 40 parents of beta-thalassemia patients recruited for initial evaluation of ... more Mutational status of 40 parents of beta-thalassemia patients recruited for initial evaluation of HRM study. (DOCX 11 kb)

Research paper thumbnail of Pattern of Drug Resistance of Pathogenic Microbes in the Street Foods of Dhaka City, Bangladesh

The ready-to-eat (RTE) foods sold by the street vendors cause numerous public health hazards. A p... more The ready-to-eat (RTE) foods sold by the street vendors cause numerous public health hazards. A pilot study was carried out in three areas of Dhaka city, namely Dhanmondi (posh residential area), Lalbag (Low income group residential area) and Motijheel (Commercial area) to assess the microbial load of seven randomly chosen commonly consumed street-vended foods (SVFs)-Bhelpuri, Cake, Cholaboot, Ghugni, Samucha, Singara and Sugarcane juice. The mean aerobic plate count (APC), total coliform (TC) and total Escherichia coli (EC) count ranged from 2.74-3.78, 1.77-2.58 and 1.57-2.64 log 10 CFU/g respectively for all the foods tested. According to the guidelines for RTE foods, the APC for Bhelpuri, Cholaboot, Cake and Ghugni is in the satisfactory level whereas for Samucha and Singara it is in the acceptable level. However, the APC, TC and EC count for sugarcane juice are 3.55-4.16, 2.76-3.54 and 2.46-3.39 log 10 CFU/ml respectively and all these values fall in the unsatisfactory level. Taking 10 bacterial isolates randomly from each food item of all three areas, a total of 210 colonies were isolated to check the antimicrobial resistant pattern. 79.52% of isolates did not show any antibiotic resistance while the remaining 20.47% of isolates showed resistance to single/multiple antibiotics. Electrophoresis of DNA extract from antibiotic-resistant-isolates showed the presence of plasmid DNA of various sizes. One of the isolate from Bhelpuri of Dhanmondi showed resistance to ampicllin (Amp), neomycin (Neo) and penicillin (Pen) and was identified biochemically as Hafnia alvei. Another isolate from Sugarcane juice of Motijheel showed resistance to Amp, gentamycin (Gen), kanamycin (Kan), Neo and Pen and was identified biochemically as Klebsiella pneumoniae.

Research paper thumbnail of The presence of hydroquinone, and mercury in skin toning creams, as well as lead in turmeric powder, pose potential health risks to Bangladeshi women

Bioresearch Communications

Skin toning creams and turmeric powders are widely used among Bangladeshi fashion and beauty conc... more Skin toning creams and turmeric powders are widely used among Bangladeshi fashion and beauty concerned women and young girls in general. However, it has been reported that excessive use of skin toning cream and turmeric powder can cause various dermatological problems, including skin cancer. These creams and turmeric powder contain a wide variety of active ingredients, including Hydroquinone (HQ), Mercury (Hg), and Lead (Pb), that are toxic, and hazardous to health, especially after prolonged application. As a result, in the current study, we tried to assess HQ and Hg level in commonly used skin toning creams and Pb in turmeric powder in the context of Bangladeshi women collected from the local markets in Dhaka city. HQ, Hg, and Pb levels were analyzed for 40 different Bangladeshi skin toning cream products and 40 different turmeric powder samples. The HQ level was determined by the high-performance liquid chromatography (HPLC) method, the Hg level was determined by Cold Vapor Atomi...

Research paper thumbnail of Detection of −α 3.7 and _ _SEA deletions in α-globin gene in the Bangladeshi population

Research Square (Research Square), Jan 2, 2024

Research paper thumbnail of Q531L mutation in the capsid protein of hepatitis E virus genotype 1 causes infections in patients with altered immunity and immunosuppressive condition: Mechanism based on wet lab and in-silico findings

Research paper thumbnail of Application of Engineering in Biomedical Science: The Window of Opportunity

Current Trends in Biomedical Engineering & Biosciences, Feb 20, 2017

Research paper thumbnail of Application of Biochemical Tests and Machine Learning Techniques to Diagnose and Evaluate Liver Disease

Advances in Bioscience and Biotechnology, 2021

Background: The liver function tests (LFTs) remain one of the most commonly employed clinical mea... more Background: The liver function tests (LFTs) remain one of the most commonly employed clinical measures for the diagnosis of hepatobiliary disease. LFTs sometimes referred to as hepatic panel help to determine the health of liver, monitor the progression of a disease and measure the severity of a disease particularly scarring or cirrhosis of the liver. Aims: In this study, we present a new approach to evaluate the natural progression of liver disease through the assessment of eight biochemical parameters: serum total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), Alkaline phosphatase (ALP), total protein (TP), albumin (ALB), albumin/globulin (A/G) ratio, and alpha-fetoprotein (AFP) as well as two machine learning (ML) tools-Random Forest and CART to substantive the outcome. Methods: The study was carried out in a total of 100 subjects which included healthy controls (group I-25 patients), patients with acute hepatitis (group II-25 patients), chronic hepatitis (group III-25 patients) and hepatocellular carcinoma (group IV-25 patients) applying both biochemical and Machine Learning methods. Results: Of the eight parameters tested, all except ALP (p = 0.426), showed an overall discriminatory ability as judged by one-factor analysis of variance (p < 0.0001). We also assessed the differences among group means by least significance difference (LSD). The analysis showed that TB remained significantly elevated in groups II, III, and IV as compared to controls (p < 0.05). ALP did not have any discriminatory power among the four groups tested. ALT and AST were good discriminators only between the control groups and groups II and III. TP, ALB, and A/G ratio were decreased significantly in groups III and IV as compared to controls. Group III and IV were almost indistinguishable using these biochemical parameters except for AFP, which was found to be elevated only in group IV.

Research paper thumbnail of Analysis of Proteotranscriptomics Landscape Reveals Differentially Regulated Pathways in &lt;i&gt;Toxoplasma gondii&lt;/i&gt; Infected Mouse Liver

Computational molecular bioscience, 2022

Toxoplasma gondii (T. gondii) an intracellular protozoan parasite, infects mammals including huma... more Toxoplasma gondii (T. gondii) an intracellular protozoan parasite, infects mammals including human population worldwide. Upon primary infection, the parasite contributes to mild flu like symptoms in immune competent host, but life threatening complication is seen in immune compromised patients and in pregnant women. Understanding the host-parasite interaction is critical for understanding the pathogenesis and biology parasite reactivation in the host. In this study, we used proteotrasncriptomics analyses by integrating the transcriptomics and proteomics data of T. gondii infected mouse liver to uncover the effector molecules responsible for disease pathogenesis that can be used as candidate markers for diagnosis and drug target. With this aim, we systematically integrated transcriptomicand proteomic data, representing the parasite infected mouse liver. Out of 2758 differentially expressed genes (DEGs) and 301 differentially expressed proteins (DEPs), 159 overlapping genes were identified. Among them, 86 genes were upregulated and 72 were downregulated in their respective mRNA and protein levels in the infected condition. Gene Ontology (GO) analysis revealed that the upregulated genes were mostly associated with immune system processes whereas the downregulated genes were involved in oxidation-reduction process and metabolism of lipid, and fatty acids. Protein-protein interaction (PPI) network analysis uncovered an interaction-hub including, Psmb8, Psmb9 and Tap1 for upregulated proteins and Cyp1A2, Cyp4A10 and Cyp3A11 for down-regulated proteins. Further studies are needed to validating these effector molecules. These molecules are likely to play a vital role in disease pathogenesis, as well as can be used as potential diagnostic marker and drug target candidates.

Research paper thumbnail of Correlations between well water and human samples for each element

<p>Spearman’s rank correlation coefficient (n = 30) for correlation between drinking well w... more <p>Spearman’s rank correlation coefficient (n = 30) for correlation between drinking well water and human urine, toenail and hair samples in Bangladesh in each element (arsenic, magnesium, calcium, strontium and barium) are shown. * and **, Statistically significant correlation (*, p<0.05; **, p<0.01).</p

Research paper thumbnail of Barium absorption in the indicated percent of weight of MF-HT

<p>After the indicated percentages of weight of MF-HT (percent of MF-HT) had been suspended... more <p>After the indicated percentages of weight of MF-HT (percent of MF-HT) had been suspended in solutions containing 1,000 µg/L barium, the MF-HT-suspended solutions were shaken at 300 rpm for 1 hour. Barium concentrations (means ± SD) in the supernatants after the solutions had been centrifuged are presented.</p

Research paper thumbnail of Exploring the Nutrition and Health Benefits of Functional Foods

This chapter focuses on the potential health benefits of camel milk including angiotension I-conv... more This chapter focuses on the potential health benefits of camel milk including angiotension I-converting enzyme-inhibitory, anti-cancer and antioxidant activities, antidiabetic, antimicrobial and hypoallergenicity effects. The bioactivity of oligosaccharide, conjugated linoleic acid and D-amino acid in camel milk is provided. The proposed mechanisms behind these bioactive components and potential health claims are explained. This chapter also describes camel milk composition, nutritional value, production and population. The current available information in the literature on camel milk is not abundant. More research is needed to give better understanding on functional properties of camel milk.

Research paper thumbnail of Association of genetic variants located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes with type 2 Diabetes Mellitus in Bangladeshi population

Background Genome-Wide Association Studies have identified genetic polymorphisms located at the A... more Background Genome-Wide Association Studies have identified genetic polymorphisms located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes on chromosome 3 as susceptibility loci for type 2 diabetes mellitus (T2DM) in different population. The present study investigates the association of these two susceptibility loci (rs11708067 at the ADCY5 and rs11926707 at the PTH1R gene) with T2DM in Bangladeshi population. Furthermore, the association of circulating levels of vitamin D, Calcium (Ca), phosphate, and parathyroid hormone (PTH) with T2DM and the different genotypes of these SNPs were also studied.Methods and Materials A total of 415 individuals (211 T2DM patients and 204 healthy controls) were enrolled in the current study. The rs11708067 and rs11926707 were genotyped using the Taqman SNP Genotyping Assays. Univariate logistic regression analyses were performed for parameter associations with T2DM.Results In a sex-stratified analysis, the variant a...

Research paper thumbnail of Additional file 7: Figure S6. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Temperature shifted difference curves of unknown carrier parents subjected to HRM analysis by 2nd... more Temperature shifted difference curves of unknown carrier parents subjected to HRM analysis by 2nd set of primers. (TIFF 487 kb)

Research paper thumbnail of Additional file 4: Figure S3. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Normalized melt curve patterns of heterozygous c.27_28insG, c.46delT, c.47Gâ >â A and c.92Gâ &... more Normalized melt curve patterns of heterozygous c.27_28insG, c.46delT, c.47Gâ >â A and c.92Gâ >â C mutations. (TIFF 211 kb)

Research paper thumbnail of Additional file 3: Figure S2. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Normalized melt curve patterns of homozygous and heterozygous c.126_129delCTTT mutations. (TIFF 1... more Normalized melt curve patterns of homozygous and heterozygous c.126_129delCTTT mutations. (TIFF 173 kb)

Research paper thumbnail of Additional file 2: Figure S1. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Normalized melt curve patterns of homozygous and heterozygous c.79G > A and c.92 + 5G > C m... more Normalized melt curve patterns of homozygous and heterozygous c.79G > A and c.92 + 5G > C mutations. (TIFF 232 kb)

Research paper thumbnail of MOESM1 of Nationwide carrier detection and molecular characterization of β-thalassemia and hemoglobin E variants in Bangladeshi population

Additional file 1: Table S1. Hematological features of the participants having high HbA2 level (H... more Additional file 1: Table S1. Hematological features of the participants having high HbA2 level (HbA2 > 3.5%) without mutation in beta globin gene of hemoglobin.

Research paper thumbnail of TYPES OF ROS , SOURCE OF SYNTHESIS AND THE DAMAGE CAUSED BY THE PRODUCTION OF ROS 2 . 1 . Types of Reactive Species

Redox degenerative reactions of the biological system inevitably produce reactive oxygen species ... more Redox degenerative reactions of the biological system inevitably produce reactive oxygen species (ROS) and their derivatives. Oxidative stress is the result of an imbalance in pro-oxidant/antioxidant homeostasis that leads to the generation of toxic reactive oxygen species (ROS), such as hydrogen peroxide, organic hydro peroxides, nitric oxide, superoxide and hydroxyl radicals etc. Information are accumulating steadily, supporting the general importance of oxidative damage of tissue and cellular components as a primary or secondary causative factor in many different human diseases and aging processes. Many of the recent landmarks in scientific research have shown that in human beings, oxidative stress has been implicated in the progression of major health problems by inactivating the metabolic enzymes and damaging important cellular components, oxidizing the nucleic acids, leading to cardiovascular diseases, eye disorders, joint disorders, neurological diseases (Alzheimer’s disease,...

Research paper thumbnail of Molecular Diagnostic Approach Prevails Superior Over Conventional GelElectrophoresis Method in Detecting Sickle Cell Anemia

Journal of Molecular Biomarkers & Diagnosis, 2018

Sickle cell anemia is defined as homozygosity caused by the mutation of the glutamic acid residue... more Sickle cell anemia is defined as homozygosity caused by the mutation of the glutamic acid residue to valine in the β-globin gene. Sickle cell disease is an increasing global health burden with the estimated number of patients increasing in a concerning manner. Here we report a very interesting and clinically insightful case of hemoglobinopathy which was initially suspected to be Hb S/D Punjab-a rare type of hemoglobinopathy as diagnosed by the hemoglobinelectrophoresis technique. Despite the diagnosis report indicating the rare Hb S/D Punjab, the uncharacteristic clinical presentation of the patient which was not coherent with the classical symptoms of Hb S/D Punjab, forced the clinicians to turn their attention to molecular diagnosis. To clarify the etiology of the clinical case, a sequencing-based molecular diagnosis approach was adopted that revealed the mutational signature of sickle cell anemia (SCA). This case can be regarded as a prominent example where the molecular techniques lead to the correct diagnosis matching with the clinical symptoms while the conventional diagnostic approach failed.

Research paper thumbnail of A computational in silico approach to predict high-risk coding and non-coding SNPs of human PLCG1 gene

PLoS ONE, 2021

PLCG1 gene is responsible for many T-cell lymphoma subtypes, including peripheral T-cell lymphoma... more PLCG1 gene is responsible for many T-cell lymphoma subtypes, including peripheral T-cell lymphoma (PTCL), angioimmunoblastic T-cell lymphoma (AITL), cutaneous T-cell lymphoma (CTCL), adult T-cell leukemia/lymphoma along with other diseases. Missense mutations of this gene have already been found in patients of CTCL and AITL. The non-synonymous single nucleotide polymorphisms (nsSNPs) can alter the protein structure as well as its functions. In this study, probable deleterious and disease-related nsSNPs in PLCG1 were identified using SIFT, PROVEAN, PolyPhen-2, PhD-SNP, Pmut, and SNPS&GO tools. Further, their effect on protein stability was checked along with conservation and solvent accessibility analysis by I-mutant 2.0, MUpro, Consurf, and Netsurf 2.0 server. Some SNPs were finalized for structural analysis with PyMol and BIOVIA discovery studio visualizer. Out of the 16 nsSNPs which were found to be deleterious, ten nsSNPs had an effect on protein stability, and six mutations (L41...

Research paper thumbnail of Additional file 1: Table S1. of High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Mutational status of 40 parents of beta-thalassemia patients recruited for initial evaluation of ... more Mutational status of 40 parents of beta-thalassemia patients recruited for initial evaluation of HRM study. (DOCX 11 kb)

Research paper thumbnail of Pattern of Drug Resistance of Pathogenic Microbes in the Street Foods of Dhaka City, Bangladesh

The ready-to-eat (RTE) foods sold by the street vendors cause numerous public health hazards. A p... more The ready-to-eat (RTE) foods sold by the street vendors cause numerous public health hazards. A pilot study was carried out in three areas of Dhaka city, namely Dhanmondi (posh residential area), Lalbag (Low income group residential area) and Motijheel (Commercial area) to assess the microbial load of seven randomly chosen commonly consumed street-vended foods (SVFs)-Bhelpuri, Cake, Cholaboot, Ghugni, Samucha, Singara and Sugarcane juice. The mean aerobic plate count (APC), total coliform (TC) and total Escherichia coli (EC) count ranged from 2.74-3.78, 1.77-2.58 and 1.57-2.64 log 10 CFU/g respectively for all the foods tested. According to the guidelines for RTE foods, the APC for Bhelpuri, Cholaboot, Cake and Ghugni is in the satisfactory level whereas for Samucha and Singara it is in the acceptable level. However, the APC, TC and EC count for sugarcane juice are 3.55-4.16, 2.76-3.54 and 2.46-3.39 log 10 CFU/ml respectively and all these values fall in the unsatisfactory level. Taking 10 bacterial isolates randomly from each food item of all three areas, a total of 210 colonies were isolated to check the antimicrobial resistant pattern. 79.52% of isolates did not show any antibiotic resistance while the remaining 20.47% of isolates showed resistance to single/multiple antibiotics. Electrophoresis of DNA extract from antibiotic-resistant-isolates showed the presence of plasmid DNA of various sizes. One of the isolate from Bhelpuri of Dhanmondi showed resistance to ampicllin (Amp), neomycin (Neo) and penicillin (Pen) and was identified biochemically as Hafnia alvei. Another isolate from Sugarcane juice of Motijheel showed resistance to Amp, gentamycin (Gen), kanamycin (Kan), Neo and Pen and was identified biochemically as Klebsiella pneumoniae.

Research paper thumbnail of The presence of hydroquinone, and mercury in skin toning creams, as well as lead in turmeric powder, pose potential health risks to Bangladeshi women

Bioresearch Communications

Skin toning creams and turmeric powders are widely used among Bangladeshi fashion and beauty conc... more Skin toning creams and turmeric powders are widely used among Bangladeshi fashion and beauty concerned women and young girls in general. However, it has been reported that excessive use of skin toning cream and turmeric powder can cause various dermatological problems, including skin cancer. These creams and turmeric powder contain a wide variety of active ingredients, including Hydroquinone (HQ), Mercury (Hg), and Lead (Pb), that are toxic, and hazardous to health, especially after prolonged application. As a result, in the current study, we tried to assess HQ and Hg level in commonly used skin toning creams and Pb in turmeric powder in the context of Bangladeshi women collected from the local markets in Dhaka city. HQ, Hg, and Pb levels were analyzed for 40 different Bangladeshi skin toning cream products and 40 different turmeric powder samples. The HQ level was determined by the high-performance liquid chromatography (HPLC) method, the Hg level was determined by Cold Vapor Atomi...