Shelly Sharma - Academia.edu (original) (raw)
Papers by Shelly Sharma
World Academy of Science, Engineering and Technology, International Journal of Medical and Health Sciences, 2016
Environmental science and pollution research international, 2017
The study was aimed to evaluate the levels of chlorpyrifos (CPF) pollution in agricultural soil o... more The study was aimed to evaluate the levels of chlorpyrifos (CPF) pollution in agricultural soil of Punjab, India, its detrimental effects on acetylcholinesterase (AChE) activity in rat brain and bioremediation of soils polluted with CPF using indigenous and adapted bacterial lab isolate. The analysis revealed that soil samples of Bathinda and Amritsar regions are highly contaminated with chlorpyrifos showing 19 to 175 mg/kg concentrations of CPF. The non-targeted animals may get poisoned with CPF by its indirect dermal absorption, inhalation of toxic fumes and regular consumption of soiled food grains. The study indicated that even the lowermost concentrations of CPF, 19 and 76 mg/kg of soil found in the Amritsar and Bathinda regions respectively can significantly inhibit the AChE activity in rat brain within 24 h of its treatment. This represents the antagonistic effect of CPF on AChE which is a prime neurotransmitter present in all living beings including humans. In light of this,...
Environmental Science and Pollution Research, 2019
This study was aiming to treat the chlorpyrifos (CPF), an organophosphate (OP) pesticide with mic... more This study was aiming to treat the chlorpyrifos (CPF), an organophosphate (OP) pesticide with microbial enzyme extract, and assess the toxicity effects of CPF before/after its treatment on the integrity of DNA (deoxyribonucleic acid) and the activities of enzymes AChE (acetylcholinestrase), GST (glutathione S-transferase), SOD (superoxide dismutase), CAT (catalase), and MDA (malondialdehyde) in different organs of rat. The untreated CPF in rat significantly increased the DNA damage and decreased the activities of all these enzymes. Among all the organs studied, the liver was the most affected organ. Further, CPF was treated with an OPH (organophosphate hydrolase) enzyme obtained from CPF degrading bacterial laboratory isolate Pseudomonas sp. (ChlD) to neutralize the toxicity of CPF. The crude intracellular enzyme extract degraded > 90% of added CPF and > 80% of its toxic intermediate 3,5,6-trichloropyridinol (TCP) which resulted in > 80% reduction of CPF toxicity in different organs of rat. Thus, this study not only illustrated the adverse effect of OPs on mammalian system but also suggested a highly efficient and ecofriendly way to remove the harmful pesticide from the environment and agricultural food products which may help to reduce the exposure of humans to such lethal toxicants.
Pesticides are being purposely added to the environment to control domestic and agricultural pest... more Pesticides are being purposely added to the environment to control domestic and agricultural pests for increasing the yield of crops to satisfy the growing demands of population. The present study was aimed to evaluate the ameliorative effect of cow urine on chlorpyrifos-induced genotoxcity in vivo. Male healthy rats, 8-10 weeks old, weighing 120 ± 10 g were randomly selected and divided into eight groups, namely, corn oil (C); cow urine (CU),Group P-1/8 of LD50 i.e 19mg/kg b.wt CPF, Group Q-1/4 of LD50 i.e 38mg/kg b.wt CPF, Group R-1/2 of LD50 i.e 76mg/kg b.wt CPF, Group X-1/8 of LD50 i.e 76mg/kg b.wt + cow urine, Group Y-1/4 of LD50 i.e 38mg/kg b.wt + cow urine, Group Z-1/2 of LD50 i.e 19mg/kg b.wt + cow urine. All treatments were administered orally for 24, 48, 72 hours. Chlorpyrifos treated group showed increased chromosomal aberrations,as compared to controls. The groups pretreated with cow urine exhibited a significant decrease in frequency of aberrant cells as compare to the ...
![Research paper thumbnail of Assessment of Antioxidative and Ameliorative Potential of Aqueous Extract of Leaves of Thuja Orientalis (Platycladus Orientalis [L.])](https://attachments.academia-assets.com/85088312/thumbnails/1.jpg)
](Asian Journal of Pharmaceutical and Clinical Research, 2018
Objective: The main objective of the present study is to assess the antioxidative and attenuating... more Objective: The main objective of the present study is to assess the antioxidative and attenuating potential of aqueous extract of leaves of Thuja orientalis (AET) against the genotoxicity induced by chlorpyrifos (CPF) (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate).Methods: Phytochemical analysis of the extract was performed to reveal the presence of various bioactive compounds. Free radical scavenging activity was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and further genoprotective activity of AET was evaluated by in vivo studies using rat as model. For this purpose, male healthy rats, 8–10 weeks old, weighing 120±10 g, were selected and divided into three groups, namely Group 1 fed with corn oil, taken as control, Group 2 fed with CPF, and Group 3 treated with AET 1 week before CPF exposure. ¼ of LD50, i.e., 38 mg/kg CPF was used for experiment and was orally administered to rats.Results: The presence of bioactive compounds such as saponins, alkaloids...
SpringerPlus, 2016
Background The widespread use of pesticides in public health protection and agriculture pest cont... more Background The widespread use of pesticides in public health protection and agriculture pest control has caused severe environmental pollution and health hazards to target and nontarget organisms (Noaishi et al. 2013). Their extensive application in modern agriculture requires an intensive investigation of the impact of these chemicals on the environment and public health. Due to the disposal of hundreds of millions of kilograms of pesticides each year, these agents must be analysed for their carcinogenic properties. The everincreasing use of pesticides at an alarming rate and their unintended toxic effects on non-target organisms has raised an important global health concern. Organophosphate compounds are one of the most widely used insecticides accounting for approximately 50 % of the insecticides used globally. Though organophosphate pesticides have already
World Academy of Science, Engineering and Technology, International Journal of Medical and Health Sciences, 2016
Environmental science and pollution research international, 2017
The study was aimed to evaluate the levels of chlorpyrifos (CPF) pollution in agricultural soil o... more The study was aimed to evaluate the levels of chlorpyrifos (CPF) pollution in agricultural soil of Punjab, India, its detrimental effects on acetylcholinesterase (AChE) activity in rat brain and bioremediation of soils polluted with CPF using indigenous and adapted bacterial lab isolate. The analysis revealed that soil samples of Bathinda and Amritsar regions are highly contaminated with chlorpyrifos showing 19 to 175 mg/kg concentrations of CPF. The non-targeted animals may get poisoned with CPF by its indirect dermal absorption, inhalation of toxic fumes and regular consumption of soiled food grains. The study indicated that even the lowermost concentrations of CPF, 19 and 76 mg/kg of soil found in the Amritsar and Bathinda regions respectively can significantly inhibit the AChE activity in rat brain within 24 h of its treatment. This represents the antagonistic effect of CPF on AChE which is a prime neurotransmitter present in all living beings including humans. In light of this,...
Environmental Science and Pollution Research, 2019
This study was aiming to treat the chlorpyrifos (CPF), an organophosphate (OP) pesticide with mic... more This study was aiming to treat the chlorpyrifos (CPF), an organophosphate (OP) pesticide with microbial enzyme extract, and assess the toxicity effects of CPF before/after its treatment on the integrity of DNA (deoxyribonucleic acid) and the activities of enzymes AChE (acetylcholinestrase), GST (glutathione S-transferase), SOD (superoxide dismutase), CAT (catalase), and MDA (malondialdehyde) in different organs of rat. The untreated CPF in rat significantly increased the DNA damage and decreased the activities of all these enzymes. Among all the organs studied, the liver was the most affected organ. Further, CPF was treated with an OPH (organophosphate hydrolase) enzyme obtained from CPF degrading bacterial laboratory isolate Pseudomonas sp. (ChlD) to neutralize the toxicity of CPF. The crude intracellular enzyme extract degraded > 90% of added CPF and > 80% of its toxic intermediate 3,5,6-trichloropyridinol (TCP) which resulted in > 80% reduction of CPF toxicity in different organs of rat. Thus, this study not only illustrated the adverse effect of OPs on mammalian system but also suggested a highly efficient and ecofriendly way to remove the harmful pesticide from the environment and agricultural food products which may help to reduce the exposure of humans to such lethal toxicants.
Pesticides are being purposely added to the environment to control domestic and agricultural pest... more Pesticides are being purposely added to the environment to control domestic and agricultural pests for increasing the yield of crops to satisfy the growing demands of population. The present study was aimed to evaluate the ameliorative effect of cow urine on chlorpyrifos-induced genotoxcity in vivo. Male healthy rats, 8-10 weeks old, weighing 120 ± 10 g were randomly selected and divided into eight groups, namely, corn oil (C); cow urine (CU),Group P-1/8 of LD50 i.e 19mg/kg b.wt CPF, Group Q-1/4 of LD50 i.e 38mg/kg b.wt CPF, Group R-1/2 of LD50 i.e 76mg/kg b.wt CPF, Group X-1/8 of LD50 i.e 76mg/kg b.wt + cow urine, Group Y-1/4 of LD50 i.e 38mg/kg b.wt + cow urine, Group Z-1/2 of LD50 i.e 19mg/kg b.wt + cow urine. All treatments were administered orally for 24, 48, 72 hours. Chlorpyrifos treated group showed increased chromosomal aberrations,as compared to controls. The groups pretreated with cow urine exhibited a significant decrease in frequency of aberrant cells as compare to the ...
Asian Journal of Pharmaceutical and Clinical Research, 2018
Objective: The main objective of the present study is to assess the antioxidative and attenuating... more Objective: The main objective of the present study is to assess the antioxidative and attenuating potential of aqueous extract of leaves of Thuja orientalis (AET) against the genotoxicity induced by chlorpyrifos (CPF) (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate).Methods: Phytochemical analysis of the extract was performed to reveal the presence of various bioactive compounds. Free radical scavenging activity was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and further genoprotective activity of AET was evaluated by in vivo studies using rat as model. For this purpose, male healthy rats, 8–10 weeks old, weighing 120±10 g, were selected and divided into three groups, namely Group 1 fed with corn oil, taken as control, Group 2 fed with CPF, and Group 3 treated with AET 1 week before CPF exposure. ¼ of LD50, i.e., 38 mg/kg CPF was used for experiment and was orally administered to rats.Results: The presence of bioactive compounds such as saponins, alkaloids...
SpringerPlus, 2016
Background The widespread use of pesticides in public health protection and agriculture pest cont... more Background The widespread use of pesticides in public health protection and agriculture pest control has caused severe environmental pollution and health hazards to target and nontarget organisms (Noaishi et al. 2013). Their extensive application in modern agriculture requires an intensive investigation of the impact of these chemicals on the environment and public health. Due to the disposal of hundreds of millions of kilograms of pesticides each year, these agents must be analysed for their carcinogenic properties. The everincreasing use of pesticides at an alarming rate and their unintended toxic effects on non-target organisms has raised an important global health concern. Organophosphate compounds are one of the most widely used insecticides accounting for approximately 50 % of the insecticides used globally. Though organophosphate pesticides have already