Sherry Glover - Academia.edu (original) (raw)

Papers by Sherry Glover

Research paper thumbnail of Vaccination Against Rabbit Hemorrhagic Disease Virus 2 Using a Baculovirus Recombinant Vaccine Provides Durable Immunity in Rabbits

Research paper thumbnail of Vaccination against Rabbit Hemorrhagic Disease Virus 2 (RHDV2) Using a Baculovirus Recombinant Vaccine Provides Durable Immunity in Rabbits

Viruses, 2024

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Confirmation of Presence of Borrelia burgdorferi Outer Surface Protein C Antigen and Production of Antibodies to Borrelia burgdorferi Outer Surface Protein C in Dogs Vaccinated with a Whole-cell Borrelia burgdorferi Bacterin

The findings in this study demonstrate the presence of Borrelia burgdorferi outer surface protein... more The findings in this study demonstrate the presence of Borrelia burgdorferi outer surface protein C (OspC) antigen in a whole-cell bacterin a (BBB) and the production of antibodies directed against Borrelia burgdorferi OspC in dogs immunized with this bacterin. Client owned dogs were determined to have negative test results for infection with Borrelia burgdorferi prior to initial or booster immunization. Monoclonal OspC antibody Western blot demonstrated presence of Borrelia burgdorferi OspC antigen in the whole-cell bacterin. Serum samples obtained post-immunization and evaluated with an in vitro qualitative assay for the detection of IgG antibodies in canine serum that react with Borrelia burgdorferi antigens, including OspC, revealed that dogs under field and laboratory conditions had Western blot evidence of production of antibodies directed against Borrelia burgdorferi OspC.

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple... more To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentag...

Research paper thumbnail of Canine Parvovirus ( CPV ) Type 2 b Vaccine Protects puppies with Maternal Antibodies to CPV when Challenged with Virulent CPV-2 c Virus

This study was conducted to evaluate canine parvovirus disease prevention efficacy of the minimum... more This study was conducted to evaluate canine parvovirus disease prevention efficacy of the minimum immunizing dose of the CPV-2b fraction of a multivalent vaccine when administered at approximately 6 weeks of age to pups with maternal CPV-2b antibodies. A second dose was administered 4 weeks later. Pups were challenged with a virulent strain of CPV-2c virus 2 months after the second vaccination. Efficacy was evaluated by monitoring the pups for various clinical observations and laboratory testing of parvovirus infection, including mucous stool, bloody stool, diarrhea, fever, death, leukopenia, lymphopenia, CPV-2b serum neutralization titer, and detection of CPV in the feces. Upon a severe challenge with a virulent CPV-2c virus, four of five (80%) control pups had at least three of four clinical signs of CPV infection while 19 of 20 (95%) vaccinated pups had not more than one sign of CPV infection. The response of the control pups confirmed the virulence of the challenge and validity ...

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

OBJECTIVE To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressin... more OBJECTIVE To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. STUDY DESIGN Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. RESULTS The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte ga...

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple... more To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentag...

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple... more To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentag...

Research paper thumbnail of Vaccination Against Rabbit Hemorrhagic Disease Virus 2 Using a Baculovirus Recombinant Vaccine Provides Durable Immunity in Rabbits

Research paper thumbnail of Vaccination against Rabbit Hemorrhagic Disease Virus 2 (RHDV2) Using a Baculovirus Recombinant Vaccine Provides Durable Immunity in Rabbits

Viruses, 2024

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Confirmation of Presence of Borrelia burgdorferi Outer Surface Protein C Antigen and Production of Antibodies to Borrelia burgdorferi Outer Surface Protein C in Dogs Vaccinated with a Whole-cell Borrelia burgdorferi Bacterin

The findings in this study demonstrate the presence of Borrelia burgdorferi outer surface protein... more The findings in this study demonstrate the presence of Borrelia burgdorferi outer surface protein C (OspC) antigen in a whole-cell bacterin a (BBB) and the production of antibodies directed against Borrelia burgdorferi OspC in dogs immunized with this bacterin. Client owned dogs were determined to have negative test results for infection with Borrelia burgdorferi prior to initial or booster immunization. Monoclonal OspC antibody Western blot demonstrated presence of Borrelia burgdorferi OspC antigen in the whole-cell bacterin. Serum samples obtained post-immunization and evaluated with an in vitro qualitative assay for the detection of IgG antibodies in canine serum that react with Borrelia burgdorferi antigens, including OspC, revealed that dogs under field and laboratory conditions had Western blot evidence of production of antibodies directed against Borrelia burgdorferi OspC.

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple... more To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentag...

Research paper thumbnail of Canine Parvovirus ( CPV ) Type 2 b Vaccine Protects puppies with Maternal Antibodies to CPV when Challenged with Virulent CPV-2 c Virus

This study was conducted to evaluate canine parvovirus disease prevention efficacy of the minimum... more This study was conducted to evaluate canine parvovirus disease prevention efficacy of the minimum immunizing dose of the CPV-2b fraction of a multivalent vaccine when administered at approximately 6 weeks of age to pups with maternal CPV-2b antibodies. A second dose was administered 4 weeks later. Pups were challenged with a virulent strain of CPV-2c virus 2 months after the second vaccination. Efficacy was evaluated by monitoring the pups for various clinical observations and laboratory testing of parvovirus infection, including mucous stool, bloody stool, diarrhea, fever, death, leukopenia, lymphopenia, CPV-2b serum neutralization titer, and detection of CPV in the feces. Upon a severe challenge with a virulent CPV-2c virus, four of five (80%) control pups had at least three of four clinical signs of CPV infection while 19 of 20 (95%) vaccinated pups had not more than one sign of CPV infection. The response of the control pups confirmed the virulence of the challenge and validity ...

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

OBJECTIVE To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressin... more OBJECTIVE To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. STUDY DESIGN Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. RESULTS The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte ga...

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple... more To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentag...

Research paper thumbnail of Canine peripheral blood lymphocyte phenotyping by 7-color multiparameter flow cytometry

Analytical and quantitative cytopathology and histopathology, 2013

To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple... more To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. The high number of samples and acquired flow data (averaging 1.9 x 10(5) cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gammadelta TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentag...