Anil R Shet - Academia.edu (original) (raw)
Papers by Anil R Shet
Materials Science Forum, 2022
This research work was designed to extract essential oil from sweet lime peel (solid waste). The ... more This research work was designed to extract essential oil from sweet lime peel (solid waste). The feed material selected for study is sweet lime peel. The disposal of waste product such as peels often has a legal restriction problem and treatment process involved requires higher costs. Transformation of this waste in to useful products could cut global treatment costs. Hence the objective of our experimental work was to convert sweet lime peel in to essential oil which is in huge demand in food, pharma and cosmetic industry. In our experimental work eextraction of essential oil was evaluated and comparison study was made between steam distillation, hydro-distillation, solvent extraction and hydro-steam distillation. Maximum extraction yield of 2.4 % was achieved at 90 min of contact by hydro-distillation. Hydro-distillation method offers advantages in comparison with other three methods in terms of energy saving, cleanliness and reduced waste water. Chemical constituents of essential...
Proceedings of the Institution of Civil Engineers - Energy, 2022
Globally there is a quantum rise in production of biodiesel due to its ability to mitigate greenh... more Globally there is a quantum rise in production of biodiesel due to its ability to mitigate greenhouse gases. However, the production of biodiesel faces a major hurdle in obtaining high purity and easy separation of the main product from glycerol. Researchers worldwide are focusing on finding new sophisticated materials that may help to develop a sustainable eco-efficient process. Only a limited number of review articles have been published that cover the use of a catalyst and co-solvent for easy separation of biodiesel from glycerol. In this paper, assessment of various catalysts (homogeneous and heterogeneous), ionic liquids and deep eutectic solvents (green solvents) for biodiesel production is reviewed. This will help researchers to find an excellent combination and economic feasibility for biodiesel manufacturing meeting environmental standards. This will provide a solid background for future research.
Bulletin of Materials Science, 2013
Optimization of process parameters for synthesis of MWCNTs from renewable plant based green precu... more Optimization of process parameters for synthesis of MWCNTs from renewable plant based green precursors using Taguchi robust method The prerequisite for process optimization is to set an objective parameter. For the synthesis of MWCNTs it is necessary to optimize the process for the objective parameter such as better yield or quality. This needs to be done for each precursor type. To achieve the twin objective of physical optimization of the process parameters for better yield and acceptable quality for each precursor type poses almost an impossible task because of time and cost involved. However, a statistical technique such as Taguchi robust technique is expected to be an efficient and effective tool to do this. The process needs to be optimized for each of the precursors selected for MWCNT synthesis for higher yield and acceptable relative quality conforming to requirement of applications. In this chapter, a few parameters that influence the yield and quality of CNT have been identified. Taguchi robust technique is employed which not only helps to optimize the parameters but also analyse the extent of effect of each parameter on the synthesis. 3.1. Introduction In the earlier chapter, the potency of six plant based precursors for CNT synthesis was outlined with details of synthesis and characterization of CNTs which were multiwalled in nature. The yield and quality of those MWCNTs depend on various process parameters like temperature of synthesis, flow rate of carrier gas, catalyst type and characteristics. Thus, it becomes very important to explore the optimum conditions for better yield and acceptable quality for synthesis of MWCNTs from the six screened plant precursors. The work in this chapter is an attempt to use Taguchi robust technique for determining the optimum conditions for MWCNT synthesis form plant based precursors.
Journal of Engineering Education Transformations
Unit Operations is the hallmark course of Chemical Engineering and allied disciplines. This invol... more Unit Operations is the hallmark course of Chemical Engineering and allied disciplines. This involves the separation of products by physical means such as Distillation, Extraction, Gas absorption, Crystallization, Evaporation, Filtration, etc. Unit Operations course is usually offered in the second year of the undergraduate program in Biotechnology. The conventional pedagogy practiced to date was not so effective in the attainment of certain Program Outcomes (PO). To address this issue, categorization of the laboratory was done into four types: Demonstration, Exercise, Structural inquiry, and Open-ended experiments was made. The experiments were carefully designed to address some of the major Technical and Professional outcomes. Assessment criteria for each kind of experiment were mapped to Program outcomes, Outcome elements, and Performance Indicators. This helps in the evaluation of the attainment of Program Outcomes more accurately. The present study deals with an active and colla...
Science of Advanced Materials
The primary goal of this study is to convert waste cooking oil into biodiesel using immobilized l... more The primary goal of this study is to convert waste cooking oil into biodiesel using immobilized lipase enzyme from Bacillus halotolerans. The used cooking oil acid quantity is 3.95 mg KOH/g. In the optimal settings of enzymatic transesterification, methanol:oil ratio 6:1, reaction time 120 min, lipase enzyme concentration 0.6 mg/ml, and temperature 32 °C, the biodiesel yield was 29.64 mg/kg. ANOVA analysis revealed that enzyme concentration and methanol:oil ratios are the two most dominating factors affecting biodiesel yield. The high R2 99.87 and R2 adj 99.56 values indicate that the fitted model agrees with the predicted biodiesel and actual experimental values. Bio-supported beads could preserve up to 76.62 percent of original activity after six cycles of immobilized lipase enzyme reusability testing. Furthermore, the characteristics of biodiesel were assessed and confirmed using the glycerol assay method and FTIR analysis. The proposed technique has the potential to reduce biodi...
Separations, Dec 13, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Fermentation
Pectinolytic enzymes are related enzymes that hydrolyze pectic substances. Pectinolytic enzymes a... more Pectinolytic enzymes are related enzymes that hydrolyze pectic substances. Pectinolytic enzymes are of great interest in industrial applications for softening fruits, extracting and clarifying juices, extracting olive oil, retting textile fibers, preparing gel, and isolating protoplasts. The current work presents acidic extracellular pectinase production using low-cost agro-industrial waste with the indigenously isolated novel strain Aspergillus cervinus. Two fungal isolates, ARS2 and ARS8, with maximum pectinase activity, 41.88 ± 1.57 IU/mL and 39.27 ± 1.14 IU/mL, respectively, were screened out of 27 isolates from decayed fruit peels (orange, banana, and lemon) and soil containing decomposed vegetables. The isolate ARS2, identified as Aspergillus cervinus by molecular characterization, showed the highest pectinase activity of 43.05 ± 1.38IU/mL during screening and was further used for media component screening and optimization studies. To understand their effect on pectinase activ...
Journal of Engineering Education Transformations, 2020
Cardiovascular disease is among the largest contributors of premature mortality in the world caus... more Cardiovascular disease is among the largest contributors of premature mortality in the world caused by inflammation of blood vessels. The abnormalities provoke thrombus formation or thrombosis blocking blood vessels leading to strokes, heart attacks and coronary artery diseases. Increasing percentage of cardiovascular cases and deaths due to thrombosis has attracted researchers to look for newer thrombolysis agents. Commonly used drugs to treat thrombosis has been limited due to various side effects. Therefore, the search for sources of safer and cheaper fibrinolytic enzymes for handling thrombolysis continues. This study aimed to evaluate potentials of fibrinolytic protease of bacteria isolated from fermented seafood (sea cucumber) products as antithrombotic agents. Information was initially gathered from scientific publications identified using web-based tools including PubMed (National Center for Biotechnology Information), Science Direct (Scopus) and Web of Science (Thomson Reuters) using combinations of search terms including "fibrinolytic enzyme protease", "endopeptidase", "fermented food", "sea cucumber", "thrombolysis therapy," "thrombolytic agent," "fibrinolytic bacteria," "fibrinolysis," "protease producing bacteria," "fibrin degradation," "holothurians," etc. We also searched for these terms in national and international organization technical reports and databases. This literature review reveals the prospects of fibrinolytic protease enzymes from bacteria from fermented seafood, particularly sea cucumber as novel antithrombotic agents.
Journal of Pharmaceutical Research International, 2022
Cellulase was produced by Cellulomonas uda utilizing banana stem waste by solid state fermentatio... more Cellulase was produced by Cellulomonas uda utilizing banana stem waste by solid state fermentation (SSF). The different parameters effects considered like particle size of substrate, pH(incubation and extraction), incubation period, temperature (incubation and extraction), media components (yeast extract and peptone) and moisture of substrate on the production of cellulase was investigated. The optimum activity of cellulase produced by Cellulomonas uda on banana waste for particle size (6.97 IU/min for 1mm), extraction pH (7.13 IU/min for pH 7), incubation pH(6.97 IU/min for pH 7), extraction temperature (7.10 IU/min for 500C), incubation temperature (7.20 IU/min for 450C), incubation period (7.20 IU/min on 3rd day), moisture content (7.12 IU/min for 100% ), peptone content ( 7.23 IU/min for 0.5 gm) and yeast extract content (7.18 IU/min for 0.30gm) was recorded. The cellulase produced by Cellulomonas uda by using banana stem waste indicates the socio-econic utilization of agricul...
Journal of Engineering Education Transformations, 2017
Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of al... more Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of allied subjects, good hands-on skill-sets, along with an ability to integrate biology with engineering for the graduates to be industry-ready. In this context, theme based mini and minor academic projects for V and VI semester respectively were designed and implemented for under-graduate students of Biotechnology. The objectives of the mini project were 1) To equip the students with basic skill-sets and impart the culture of Good Laboratory Practices (GLP)& Standard Operating Procedures (SOP)and 2) to impart basic microbiological and biochemical skillsets. In continuation of mini project, students have to harness the capabilities of skills learnt in mini project to translate in to an industrially viable product, hence fermentation skills play an important role. In this perspective, the objectives of minor project were developed to impart the students with 1) basic fermentation skills at la...
In recent years, the fossil fuel resources are depleting rapidly with consequent environment degr... more In recent years, the fossil fuel resources are depleting rapidly with consequent environment degradation. Further, India is importing more than 80% of its fuel requirement and spending a huge amount of foreign currency on fuel. In view of this, it becomes highly imperative to search alternative fuel options based on renewable energy. Biodiesel, the monoalkyl esters of long chain fatty acids derived from a renewable lipid feedstock’s such as vegetable oil or animal fat is providing a substitute to diesel. In the present study the parameters affecting solvent extraction and transesterification of oil was investigated. Also the characterization of the produced biodiesel was done. The highest oil extraction was obtained under the conditions of 0.425 mm particle size, 8:1 solvent to feed ratio, 6 h extraction time and extraction temperature of 60C. The results showed that biodiesel produced from soybeans was the maximum at 1:1 methanol to oil ratio, 2 h reaction time, 0.5% NaOH catalyst,...
Silver nanoparticles have attracted increasing interest due to their unique physical, chemical an... more Silver nanoparticles have attracted increasing interest due to their unique physical, chemical and biological properties compared to their macro-scaled counterparts. A comparative study of chemical and biological method for Silver nanoparticle synthesis is done. Few locally available fruits including Lemon (Citrus aurantifolia), Orange (Citrus sinensis) and Tomato (Solanum lycopersicum), which are known to contain relatively high amount of ascorbic acid were selected for silver nanoparticle synthesis. Silver nanoparticles were prepared from aqueous solution of silver nitrate using different fruit extracts. The synthesized silver nanoparticles have been characterized by UV-visible spectroscopy. The UV-Vis spectrophotometer analysis showed a peak for silver nanoparticles between 2-6 hours for the samples kept at room temperature (37ºC). It was also observed that orange extract synthesized nanoparticles showed highest peak, indicating synthesis of maximum nanoparticles and Trisodium citrate synthesized nanoparticles showed the minimum. Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method. Antibacterial activity of the synthesized silver nanoparticles was tested with one Gram-positive (Staphylococcus aureus) and one Gram-negative (Escherichia coli) bacterium. Antibacterial effect was found to be maximum for lemon extract synthesized nanoparticles, and it was found that the silver nanoparticles have antibacterial activity against Escherichia coli and Staphylococcus aureus.
Enzymes are biocatalyst, produced by living systems, are proteineous in nature with catalytic pro... more Enzymes are biocatalyst, produced by living systems, are proteineous in nature with catalytic properties Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) are a class of hydrolyses that are primarily responsible for the hydrolysis of acylglycerides. Lipases are produced by various microbes, such as bacteria, fungi, yeast, and also in the pancreas of mammals, like pigs and humans. Commercially useful lipases are usually obtained from microorganisms that produce a wide variety of extracellular lipases. Only about 2% of world’s microorganisms have been tested as enzyme sources. In the present study different oil contaminated soils were taken for isolation of lipolytic organisms. Different samples were collected and isolation of lipolytic microorganisms is done by standard plate assay method. This methodology helps in getting cultures with less contamination and abundance of bacteria can be minimized by using antibiotics in culture medium Totally seven strain were isolated, out of se...
The ability to predict the conformations and energi s involved in the binding of small molecules ... more The ability to predict the conformations and energi s involved in the binding of small molecules to pr teins is quite crucial in designing potential drugs that c n interact favourably with their target proteins. Docking is also used to predict protein-protein complexes, whi ch are helpful in determining the quaternary struct ures of intrinsically multimeric proteins as well as to gai n an understanding of the protein interaction netwo rks. The objective of our research work is to predict protei n ligand interaction for snake venom. Phylogenetic analysis was carried out using ClustalW2 software. ClustalW2 is a general purpose multiple sequence alignment program for DNA or proteins. Homology mod elling was done using the SWISS-MODEL Workspace. Tropine was selected as ligand for perfo rming docking. Docking was performed to find the inhibition activity of tropine against snake venom using Medock & Hex. Vibrational analysis was perfor med to check the energy changes and it was found tha...
Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of al... more Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of allied subjects along with good hands-on skill-sets for the graduates to be industry-ready. In this context, theme-based mini project for V semester was designed and implemented for under-graduate students of Biotechnology. The objectives of the mini project were 1)To equip the students with basic skill sets and impart the culture of Good Laboratory Practices (GLP)& Standard Operating Procedures (SOP) and 2) To impart basic microbiological and biochemical skill-sets. The mini project was undertaken with the theme of "Isolation, Screening and Preliminary Characterization of Microorganisms Producing Selected Industrially Important Metabolites". A multi-level rubrics-based assessment was followed to measure the learning. The project addressed eight Program Outcomes (PO) whose attainment on a scale of 10 was calculated. It is concluded that the theme-based project was effective in ex...
Journal of Engineering Education Transformations, 2021
Bio-Process Control is the application of automatic control in the field of Biotechnology. The co... more Bio-Process Control is the application of automatic control in the field of Biotechnology. The course emphasizes on the dynamic behaviour, physical and empirical modeling of bio-systems and advanced control strategies. The primary objective of process control is to maintain a process at the desired operating conditions with quality standards in an economic way and with safety aspects in place. In this backdrop, the present study was implemented as an exercise and structured inquiry experiments in Bioprocess Control Laboratory for VI semester undergraduate students of Biotechnology engineering. The course was hitherto taught in a conventional approach with analysis and interpretation of dynamic responses of different systems with standard inputs. The delivery of the exercise was implemented in three phases namely training, execution and assessment phase. In the present study dynamic response of control system was predicted with models and then compared with experimental observations. The resulting deviations therein, were analyzed for the possible underlying reasons and a chisquare goodness of fit test was performed to validate the model. The assessment of the students' performance was done on individual basis through a Rubrics-based approach and attainment of the Program outcomes addressed was recorded which ranged from 80-95%. The study helped in addressing the graduate attributes related to the investigation of complex problem, analyze the correlation of the experimental outcomes with underlying theoretical concepts and interpretation of results. A formal feedback from the students revealed that the active learning approach enhanced the understanding of the control engineering concepts through the application of statistical modeling and analysis.
bioscipub.com
Page 1. 523 Enrichment of Saccharomyces cerevisiae with zinc and their impact on cell growth Anil... more Page 1. 523 Enrichment of Saccharomyces cerevisiae with zinc and their impact on cell growth Anil R. Shet, Laxmikant R. Patil, Veeranna S. Hombalimath, Deepak A. Yaraguppi, Basavaraj B. Udapudi Department of Biotechnology ...
Microbial Pectinase has drawn great attention from various researchers worldwide as a biological ... more Microbial Pectinase has drawn great attention from various researchers worldwide as a biological catalyst in a variety of industrial processes. Pectinase is an enzyme that breaks down pectin, a polysaccharide found in plant cell walls. Pectinolytic enzymes are classified as Protopectinases, Esterases and Depolymerases according to their mode of attack on the galacturonan part of the pectin molecules. Pectinolytic enzymes are among the most important industrial enzymes with wide-ranging applications in fruit juice industry, degumming of plant bast fibers, wastewater treatment, wine industry, textile processing, paper making and coffee and tea fermentations. The present review discusses the structure, properties and applications of pectin, classification of Pectinase, production of microbial Pectinase, purification and biotechnological applications of microbial Pectinase.
Materials Science Forum, 2022
This research work was designed to extract essential oil from sweet lime peel (solid waste). The ... more This research work was designed to extract essential oil from sweet lime peel (solid waste). The feed material selected for study is sweet lime peel. The disposal of waste product such as peels often has a legal restriction problem and treatment process involved requires higher costs. Transformation of this waste in to useful products could cut global treatment costs. Hence the objective of our experimental work was to convert sweet lime peel in to essential oil which is in huge demand in food, pharma and cosmetic industry. In our experimental work eextraction of essential oil was evaluated and comparison study was made between steam distillation, hydro-distillation, solvent extraction and hydro-steam distillation. Maximum extraction yield of 2.4 % was achieved at 90 min of contact by hydro-distillation. Hydro-distillation method offers advantages in comparison with other three methods in terms of energy saving, cleanliness and reduced waste water. Chemical constituents of essential...
Proceedings of the Institution of Civil Engineers - Energy, 2022
Globally there is a quantum rise in production of biodiesel due to its ability to mitigate greenh... more Globally there is a quantum rise in production of biodiesel due to its ability to mitigate greenhouse gases. However, the production of biodiesel faces a major hurdle in obtaining high purity and easy separation of the main product from glycerol. Researchers worldwide are focusing on finding new sophisticated materials that may help to develop a sustainable eco-efficient process. Only a limited number of review articles have been published that cover the use of a catalyst and co-solvent for easy separation of biodiesel from glycerol. In this paper, assessment of various catalysts (homogeneous and heterogeneous), ionic liquids and deep eutectic solvents (green solvents) for biodiesel production is reviewed. This will help researchers to find an excellent combination and economic feasibility for biodiesel manufacturing meeting environmental standards. This will provide a solid background for future research.
Bulletin of Materials Science, 2013
Optimization of process parameters for synthesis of MWCNTs from renewable plant based green precu... more Optimization of process parameters for synthesis of MWCNTs from renewable plant based green precursors using Taguchi robust method The prerequisite for process optimization is to set an objective parameter. For the synthesis of MWCNTs it is necessary to optimize the process for the objective parameter such as better yield or quality. This needs to be done for each precursor type. To achieve the twin objective of physical optimization of the process parameters for better yield and acceptable quality for each precursor type poses almost an impossible task because of time and cost involved. However, a statistical technique such as Taguchi robust technique is expected to be an efficient and effective tool to do this. The process needs to be optimized for each of the precursors selected for MWCNT synthesis for higher yield and acceptable relative quality conforming to requirement of applications. In this chapter, a few parameters that influence the yield and quality of CNT have been identified. Taguchi robust technique is employed which not only helps to optimize the parameters but also analyse the extent of effect of each parameter on the synthesis. 3.1. Introduction In the earlier chapter, the potency of six plant based precursors for CNT synthesis was outlined with details of synthesis and characterization of CNTs which were multiwalled in nature. The yield and quality of those MWCNTs depend on various process parameters like temperature of synthesis, flow rate of carrier gas, catalyst type and characteristics. Thus, it becomes very important to explore the optimum conditions for better yield and acceptable quality for synthesis of MWCNTs from the six screened plant precursors. The work in this chapter is an attempt to use Taguchi robust technique for determining the optimum conditions for MWCNT synthesis form plant based precursors.
Journal of Engineering Education Transformations
Unit Operations is the hallmark course of Chemical Engineering and allied disciplines. This invol... more Unit Operations is the hallmark course of Chemical Engineering and allied disciplines. This involves the separation of products by physical means such as Distillation, Extraction, Gas absorption, Crystallization, Evaporation, Filtration, etc. Unit Operations course is usually offered in the second year of the undergraduate program in Biotechnology. The conventional pedagogy practiced to date was not so effective in the attainment of certain Program Outcomes (PO). To address this issue, categorization of the laboratory was done into four types: Demonstration, Exercise, Structural inquiry, and Open-ended experiments was made. The experiments were carefully designed to address some of the major Technical and Professional outcomes. Assessment criteria for each kind of experiment were mapped to Program outcomes, Outcome elements, and Performance Indicators. This helps in the evaluation of the attainment of Program Outcomes more accurately. The present study deals with an active and colla...
Science of Advanced Materials
The primary goal of this study is to convert waste cooking oil into biodiesel using immobilized l... more The primary goal of this study is to convert waste cooking oil into biodiesel using immobilized lipase enzyme from Bacillus halotolerans. The used cooking oil acid quantity is 3.95 mg KOH/g. In the optimal settings of enzymatic transesterification, methanol:oil ratio 6:1, reaction time 120 min, lipase enzyme concentration 0.6 mg/ml, and temperature 32 °C, the biodiesel yield was 29.64 mg/kg. ANOVA analysis revealed that enzyme concentration and methanol:oil ratios are the two most dominating factors affecting biodiesel yield. The high R2 99.87 and R2 adj 99.56 values indicate that the fitted model agrees with the predicted biodiesel and actual experimental values. Bio-supported beads could preserve up to 76.62 percent of original activity after six cycles of immobilized lipase enzyme reusability testing. Furthermore, the characteristics of biodiesel were assessed and confirmed using the glycerol assay method and FTIR analysis. The proposed technique has the potential to reduce biodi...
Separations, Dec 13, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Fermentation
Pectinolytic enzymes are related enzymes that hydrolyze pectic substances. Pectinolytic enzymes a... more Pectinolytic enzymes are related enzymes that hydrolyze pectic substances. Pectinolytic enzymes are of great interest in industrial applications for softening fruits, extracting and clarifying juices, extracting olive oil, retting textile fibers, preparing gel, and isolating protoplasts. The current work presents acidic extracellular pectinase production using low-cost agro-industrial waste with the indigenously isolated novel strain Aspergillus cervinus. Two fungal isolates, ARS2 and ARS8, with maximum pectinase activity, 41.88 ± 1.57 IU/mL and 39.27 ± 1.14 IU/mL, respectively, were screened out of 27 isolates from decayed fruit peels (orange, banana, and lemon) and soil containing decomposed vegetables. The isolate ARS2, identified as Aspergillus cervinus by molecular characterization, showed the highest pectinase activity of 43.05 ± 1.38IU/mL during screening and was further used for media component screening and optimization studies. To understand their effect on pectinase activ...
Journal of Engineering Education Transformations, 2020
Cardiovascular disease is among the largest contributors of premature mortality in the world caus... more Cardiovascular disease is among the largest contributors of premature mortality in the world caused by inflammation of blood vessels. The abnormalities provoke thrombus formation or thrombosis blocking blood vessels leading to strokes, heart attacks and coronary artery diseases. Increasing percentage of cardiovascular cases and deaths due to thrombosis has attracted researchers to look for newer thrombolysis agents. Commonly used drugs to treat thrombosis has been limited due to various side effects. Therefore, the search for sources of safer and cheaper fibrinolytic enzymes for handling thrombolysis continues. This study aimed to evaluate potentials of fibrinolytic protease of bacteria isolated from fermented seafood (sea cucumber) products as antithrombotic agents. Information was initially gathered from scientific publications identified using web-based tools including PubMed (National Center for Biotechnology Information), Science Direct (Scopus) and Web of Science (Thomson Reuters) using combinations of search terms including "fibrinolytic enzyme protease", "endopeptidase", "fermented food", "sea cucumber", "thrombolysis therapy," "thrombolytic agent," "fibrinolytic bacteria," "fibrinolysis," "protease producing bacteria," "fibrin degradation," "holothurians," etc. We also searched for these terms in national and international organization technical reports and databases. This literature review reveals the prospects of fibrinolytic protease enzymes from bacteria from fermented seafood, particularly sea cucumber as novel antithrombotic agents.
Journal of Pharmaceutical Research International, 2022
Cellulase was produced by Cellulomonas uda utilizing banana stem waste by solid state fermentatio... more Cellulase was produced by Cellulomonas uda utilizing banana stem waste by solid state fermentation (SSF). The different parameters effects considered like particle size of substrate, pH(incubation and extraction), incubation period, temperature (incubation and extraction), media components (yeast extract and peptone) and moisture of substrate on the production of cellulase was investigated. The optimum activity of cellulase produced by Cellulomonas uda on banana waste for particle size (6.97 IU/min for 1mm), extraction pH (7.13 IU/min for pH 7), incubation pH(6.97 IU/min for pH 7), extraction temperature (7.10 IU/min for 500C), incubation temperature (7.20 IU/min for 450C), incubation period (7.20 IU/min on 3rd day), moisture content (7.12 IU/min for 100% ), peptone content ( 7.23 IU/min for 0.5 gm) and yeast extract content (7.18 IU/min for 0.30gm) was recorded. The cellulase produced by Cellulomonas uda by using banana stem waste indicates the socio-econic utilization of agricul...
Journal of Engineering Education Transformations, 2017
Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of al... more Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of allied subjects, good hands-on skill-sets, along with an ability to integrate biology with engineering for the graduates to be industry-ready. In this context, theme based mini and minor academic projects for V and VI semester respectively were designed and implemented for under-graduate students of Biotechnology. The objectives of the mini project were 1) To equip the students with basic skill-sets and impart the culture of Good Laboratory Practices (GLP)& Standard Operating Procedures (SOP)and 2) to impart basic microbiological and biochemical skillsets. In continuation of mini project, students have to harness the capabilities of skills learnt in mini project to translate in to an industrially viable product, hence fermentation skills play an important role. In this perspective, the objectives of minor project were developed to impart the students with 1) basic fermentation skills at la...
In recent years, the fossil fuel resources are depleting rapidly with consequent environment degr... more In recent years, the fossil fuel resources are depleting rapidly with consequent environment degradation. Further, India is importing more than 80% of its fuel requirement and spending a huge amount of foreign currency on fuel. In view of this, it becomes highly imperative to search alternative fuel options based on renewable energy. Biodiesel, the monoalkyl esters of long chain fatty acids derived from a renewable lipid feedstock’s such as vegetable oil or animal fat is providing a substitute to diesel. In the present study the parameters affecting solvent extraction and transesterification of oil was investigated. Also the characterization of the produced biodiesel was done. The highest oil extraction was obtained under the conditions of 0.425 mm particle size, 8:1 solvent to feed ratio, 6 h extraction time and extraction temperature of 60C. The results showed that biodiesel produced from soybeans was the maximum at 1:1 methanol to oil ratio, 2 h reaction time, 0.5% NaOH catalyst,...
Silver nanoparticles have attracted increasing interest due to their unique physical, chemical an... more Silver nanoparticles have attracted increasing interest due to their unique physical, chemical and biological properties compared to their macro-scaled counterparts. A comparative study of chemical and biological method for Silver nanoparticle synthesis is done. Few locally available fruits including Lemon (Citrus aurantifolia), Orange (Citrus sinensis) and Tomato (Solanum lycopersicum), which are known to contain relatively high amount of ascorbic acid were selected for silver nanoparticle synthesis. Silver nanoparticles were prepared from aqueous solution of silver nitrate using different fruit extracts. The synthesized silver nanoparticles have been characterized by UV-visible spectroscopy. The UV-Vis spectrophotometer analysis showed a peak for silver nanoparticles between 2-6 hours for the samples kept at room temperature (37ºC). It was also observed that orange extract synthesized nanoparticles showed highest peak, indicating synthesis of maximum nanoparticles and Trisodium citrate synthesized nanoparticles showed the minimum. Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method. Antibacterial activity of the synthesized silver nanoparticles was tested with one Gram-positive (Staphylococcus aureus) and one Gram-negative (Escherichia coli) bacterium. Antibacterial effect was found to be maximum for lemon extract synthesized nanoparticles, and it was found that the silver nanoparticles have antibacterial activity against Escherichia coli and Staphylococcus aureus.
Enzymes are biocatalyst, produced by living systems, are proteineous in nature with catalytic pro... more Enzymes are biocatalyst, produced by living systems, are proteineous in nature with catalytic properties Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) are a class of hydrolyses that are primarily responsible for the hydrolysis of acylglycerides. Lipases are produced by various microbes, such as bacteria, fungi, yeast, and also in the pancreas of mammals, like pigs and humans. Commercially useful lipases are usually obtained from microorganisms that produce a wide variety of extracellular lipases. Only about 2% of world’s microorganisms have been tested as enzyme sources. In the present study different oil contaminated soils were taken for isolation of lipolytic organisms. Different samples were collected and isolation of lipolytic microorganisms is done by standard plate assay method. This methodology helps in getting cultures with less contamination and abundance of bacteria can be minimized by using antibiotics in culture medium Totally seven strain were isolated, out of se...
The ability to predict the conformations and energi s involved in the binding of small molecules ... more The ability to predict the conformations and energi s involved in the binding of small molecules to pr teins is quite crucial in designing potential drugs that c n interact favourably with their target proteins. Docking is also used to predict protein-protein complexes, whi ch are helpful in determining the quaternary struct ures of intrinsically multimeric proteins as well as to gai n an understanding of the protein interaction netwo rks. The objective of our research work is to predict protei n ligand interaction for snake venom. Phylogenetic analysis was carried out using ClustalW2 software. ClustalW2 is a general purpose multiple sequence alignment program for DNA or proteins. Homology mod elling was done using the SWISS-MODEL Workspace. Tropine was selected as ligand for perfo rming docking. Docking was performed to find the inhibition activity of tropine against snake venom using Medock & Hex. Vibrational analysis was perfor med to check the energy changes and it was found tha...
Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of al... more Biotechnology being an inter-disciplinary domain, demands an adequate theoretical knowledge of allied subjects along with good hands-on skill-sets for the graduates to be industry-ready. In this context, theme-based mini project for V semester was designed and implemented for under-graduate students of Biotechnology. The objectives of the mini project were 1)To equip the students with basic skill sets and impart the culture of Good Laboratory Practices (GLP)& Standard Operating Procedures (SOP) and 2) To impart basic microbiological and biochemical skill-sets. The mini project was undertaken with the theme of "Isolation, Screening and Preliminary Characterization of Microorganisms Producing Selected Industrially Important Metabolites". A multi-level rubrics-based assessment was followed to measure the learning. The project addressed eight Program Outcomes (PO) whose attainment on a scale of 10 was calculated. It is concluded that the theme-based project was effective in ex...
Journal of Engineering Education Transformations, 2021
Bio-Process Control is the application of automatic control in the field of Biotechnology. The co... more Bio-Process Control is the application of automatic control in the field of Biotechnology. The course emphasizes on the dynamic behaviour, physical and empirical modeling of bio-systems and advanced control strategies. The primary objective of process control is to maintain a process at the desired operating conditions with quality standards in an economic way and with safety aspects in place. In this backdrop, the present study was implemented as an exercise and structured inquiry experiments in Bioprocess Control Laboratory for VI semester undergraduate students of Biotechnology engineering. The course was hitherto taught in a conventional approach with analysis and interpretation of dynamic responses of different systems with standard inputs. The delivery of the exercise was implemented in three phases namely training, execution and assessment phase. In the present study dynamic response of control system was predicted with models and then compared with experimental observations. The resulting deviations therein, were analyzed for the possible underlying reasons and a chisquare goodness of fit test was performed to validate the model. The assessment of the students' performance was done on individual basis through a Rubrics-based approach and attainment of the Program outcomes addressed was recorded which ranged from 80-95%. The study helped in addressing the graduate attributes related to the investigation of complex problem, analyze the correlation of the experimental outcomes with underlying theoretical concepts and interpretation of results. A formal feedback from the students revealed that the active learning approach enhanced the understanding of the control engineering concepts through the application of statistical modeling and analysis.
bioscipub.com
Page 1. 523 Enrichment of Saccharomyces cerevisiae with zinc and their impact on cell growth Anil... more Page 1. 523 Enrichment of Saccharomyces cerevisiae with zinc and their impact on cell growth Anil R. Shet, Laxmikant R. Patil, Veeranna S. Hombalimath, Deepak A. Yaraguppi, Basavaraj B. Udapudi Department of Biotechnology ...
Microbial Pectinase has drawn great attention from various researchers worldwide as a biological ... more Microbial Pectinase has drawn great attention from various researchers worldwide as a biological catalyst in a variety of industrial processes. Pectinase is an enzyme that breaks down pectin, a polysaccharide found in plant cell walls. Pectinolytic enzymes are classified as Protopectinases, Esterases and Depolymerases according to their mode of attack on the galacturonan part of the pectin molecules. Pectinolytic enzymes are among the most important industrial enzymes with wide-ranging applications in fruit juice industry, degumming of plant bast fibers, wastewater treatment, wine industry, textile processing, paper making and coffee and tea fermentations. The present review discusses the structure, properties and applications of pectin, classification of Pectinase, production of microbial Pectinase, purification and biotechnological applications of microbial Pectinase.