Shinzaburo Takamiya - Academia.edu (original) (raw)
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Papers by Shinzaburo Takamiya
Journal of Inherited Metabolic Disease, 1987
IEEE Transactions on Electron Devices, 1969
Medical Entomology and Zoology, 2001
Archives of Biochemistry and Biophysics, 1994
Annals of the New York Academy of Sciences, 2005
Parasites have exploited unique energy metabolic pathways as adaptations to the natural host habi... more Parasites have exploited unique energy metabolic pathways as adaptations to the natural host habitat. In fact, the respiratory systems of parasites typically show greater diversity in electron transfer pathways than do those of host animals. These unique aspects of parasite mitochondria and related enzymes may represent promising targets for chemotherapy. Natural products have been recognized as a source of the candidates of the specific inhibitors for such parasite respiratory chains. Chalcones was recently evaluated for its antimalarial activity in vitro and in vivo. However, its target is still unclear in malaria parasites. In this study, we investigated that licochalcone A inhibited the bc1 complex (ubiquinol-cytochrome c reductase) as well as complex II (succinate ubiquinone reductase, SQR) of Plasmodium falciparum mitochondria. In particular, licochalcone A inhibits bc1 complex activity at very low concentrations. Because the property of the P. falciparum bc1 complex is different from that of the mammalian host, chalcones would be a promising candidate for a new antimalarial drug.
Annals of the New York Academy of Sciences, 1986
Journal of Biological Chemistry, 1994
本研究においてデザインされた,recA遺伝子のほぼ全長に渡る領域を増幅するためのPCRプライマーを用いて得られたPCR産物の配列情報は,UPTC及び他の高温性カンピロバクターのDNAレベルでの分... more 本研究においてデザインされた,recA遺伝子のほぼ全長に渡る領域を増幅するためのPCRプライマーを用いて得られたPCR産物の配列情報は,UPTC及び他の高温性カンピロバクターのDNAレベルでの分子識別に大変有用であることが強く示唆された。更に,本研究の主目的であるUPTCのゲノムDNA上の多遺伝子配列情報に基づく分子識別にrecA遺伝子を対象とすることは極めて有用であることが初めて明らかとなった。In the present study, for the first time, nucleotide sequencing after TA cloning of almost the full-length (about 1,050 bp in length) of the recA gene of two isolates of UPTC, NCTC12894 and CF89-12 and a strain of C. lari JCM2530T amplified using a degenerate primer pair was carried out. Nucleotide sequence comparison analysis of the possible open reading frame (ORF) demonstrated that the two isolates of UPTC showed about 94% sequence homology of the recA gene to each other and that they also showed 90-92% and about 84% homology of the recA gene to C. lari and C. jejuni, respectively. The two isolates of UPTC showed 50-60% homology of the recA gene to the other family Enterobacteriaceae. In conclusion, this study presents novel sequence data on recA for C. lari and UPTC, which may aid i...
British Journal of Biomedical Science, 2006
Current Topics in Bioenergetics, 1987
Journal of Biological Chemistry, 1989
The mammalian cytochrome c oxidase is a complex of 13 different subunits. We present the full ami... more The mammalian cytochrome c oxidase is a complex of 13 different subunits. We present the full amino acid sequence of the one remaining uncharacterized subunit, subunit IHQ in the nomenclature used here, VIIb in the numbering system of Kadenbach and colleagues (e.g. Kadenbach, B., and Merle, P. (1981) FEBS Lett. 135, 1-11). A partial protein sequence was obtained from the purified subunit isolated by gel filtration procedures. This information was used to synthesize an oligonucleotide probe which was then used to isolate a cDNA clone encoding the subunit. This cDNA for subunit IHQ is 480 base pairs long and encodes a polypeptide which is either 83 or 88 amino acids long, including an N-terminal leader sequence of either 27 or 32 residues. The molecular weight of the mature subunit IHQ is 6350 based on the amino acid sequence deduced from the gene. The leader sequence is typical of other mitochondrial target sequences in having several positively charged residues but no negatively charged side chains.
British Journal of Biomedical Science, 2008
This study aims to clarify the molecular characteristics of the urease gene operon from urease-po... more This study aims to clarify the molecular characteristics of the urease gene operon from urease-positive thermophilic campylobacters (UPTC) obtained from different sources and in various countries. Sequence heterogeneity was observed for the promoter structures at the -35-like region among the 12 isolates examined. The most probable TTG start codon was suggested for the ureB and ureH genes, and for the ureA, E, F and G genes, ATG was suggested among all the isolates examined. Overlap was detected between ureA and ureB and between ureB and ureE among all the isolates examined. UPTC is the first example of an overlap between the two structural genes ureA and ureB. When the completely sequenced open reading frames (ORFs) for ureE, ureF, ureG and ureH were identified, non-coding regions between ureE and ureF, ureF and ureG, and ureG and ureH were also demonstrated. All six start codons of the six urease genes were demonstrated to be preceded by Shine-Dalgarno sequences among all the isolates examined. The Cys-His sequence corresponding to urease active sites were aligned perfectly and fully conserved among the three UPTC isolates examined. A putative and intrinsic p-independent transcriptional terminator was identified to be identical among all the isolates examined. A partial and putative ORF of about 200 bp in length showing high sequence similarity to GTP cyclohydrolase I was observed downstream of ureH.
The Journal of Biochemistry, 1978
The chemical and catalytic properties of potato phosphoglucomutase [EC 2.7.5.1] were studied usin... more The chemical and catalytic properties of potato phosphoglucomutase [EC 2.7.5.1] were studied using various enzyme species (Peaks Ia, Ib, Ic, and II; Takamiya, S. & Fukui, T. (1978) Plant Cell Physiol. 19, 319--328). The molecular weights of the species are all approximately 60,000. No indication of the presence of subunit structure was obtained under various conditions. The amino acid composition of Peak Ia is generally similar to those of the enzymes from other sources, though it has some peculiarities. The Peak Ia and Peak II enzymes both absolutely require alpha-D-glucose 1,6-bisphosphate and Mg2+ for activity, and appear to have a "ping-pong" mechanism. A low concentration of Be2+ inhibits their action, the inhibition being retarded either by Mg2 or EDTA. Although the inhibition patterns by various metabolites, are similar for Peaks Ia and II, they differ in their kinetic parameters and optimal pH values.
Plant and Cell Physiology, 1978
Parasitology International, 2016
A method for purifying active liquid-cultured Caenorhabditis elegans using a modified Baermann ap... more A method for purifying active liquid-cultured Caenorhabditis elegans using a modified Baermann apparatus is 15 described. This method consists of two parts: 1) large-scale cultivation of C. elegans in liquid medium and 2) prep-16 aration of active nematodes without contamination using the Baermann apparatus.
Cytochrome Systems, 1987
The phosphoenolpyruvate carboxykinase (PEPCK)-succinate pathway is important in the energy metabo... more The phosphoenolpyruvate carboxykinase (PEPCK)-succinate pathway is important in the energy metabolism of anaerobic organisms such as Ascaris suum, which resides in the host’s small intestine where oxygen is fairly limited (1). The last step of this pathway is catalized by fumarate reductase activity of mitochondria, and our studies on the respiratory chain of mitochondria from Ascaris indicated that complex II (succinate-ubiquinone reductase) functions as fumarate reductase by its reverse reaction. Complex II contains b-type cytochrome and properties of this cytochrome of Ascaris was somewhat different from that of beef heart. Comparative characterization of these b cytochromes are described.
Journal of Inherited Metabolic Disease, 1987
IEEE Transactions on Electron Devices, 1969
Medical Entomology and Zoology, 2001
Archives of Biochemistry and Biophysics, 1994
Annals of the New York Academy of Sciences, 2005
Parasites have exploited unique energy metabolic pathways as adaptations to the natural host habi... more Parasites have exploited unique energy metabolic pathways as adaptations to the natural host habitat. In fact, the respiratory systems of parasites typically show greater diversity in electron transfer pathways than do those of host animals. These unique aspects of parasite mitochondria and related enzymes may represent promising targets for chemotherapy. Natural products have been recognized as a source of the candidates of the specific inhibitors for such parasite respiratory chains. Chalcones was recently evaluated for its antimalarial activity in vitro and in vivo. However, its target is still unclear in malaria parasites. In this study, we investigated that licochalcone A inhibited the bc1 complex (ubiquinol-cytochrome c reductase) as well as complex II (succinate ubiquinone reductase, SQR) of Plasmodium falciparum mitochondria. In particular, licochalcone A inhibits bc1 complex activity at very low concentrations. Because the property of the P. falciparum bc1 complex is different from that of the mammalian host, chalcones would be a promising candidate for a new antimalarial drug.
Annals of the New York Academy of Sciences, 1986
Journal of Biological Chemistry, 1994
本研究においてデザインされた,recA遺伝子のほぼ全長に渡る領域を増幅するためのPCRプライマーを用いて得られたPCR産物の配列情報は,UPTC及び他の高温性カンピロバクターのDNAレベルでの分... more 本研究においてデザインされた,recA遺伝子のほぼ全長に渡る領域を増幅するためのPCRプライマーを用いて得られたPCR産物の配列情報は,UPTC及び他の高温性カンピロバクターのDNAレベルでの分子識別に大変有用であることが強く示唆された。更に,本研究の主目的であるUPTCのゲノムDNA上の多遺伝子配列情報に基づく分子識別にrecA遺伝子を対象とすることは極めて有用であることが初めて明らかとなった。In the present study, for the first time, nucleotide sequencing after TA cloning of almost the full-length (about 1,050 bp in length) of the recA gene of two isolates of UPTC, NCTC12894 and CF89-12 and a strain of C. lari JCM2530T amplified using a degenerate primer pair was carried out. Nucleotide sequence comparison analysis of the possible open reading frame (ORF) demonstrated that the two isolates of UPTC showed about 94% sequence homology of the recA gene to each other and that they also showed 90-92% and about 84% homology of the recA gene to C. lari and C. jejuni, respectively. The two isolates of UPTC showed 50-60% homology of the recA gene to the other family Enterobacteriaceae. In conclusion, this study presents novel sequence data on recA for C. lari and UPTC, which may aid i...
British Journal of Biomedical Science, 2006
Current Topics in Bioenergetics, 1987
Journal of Biological Chemistry, 1989
The mammalian cytochrome c oxidase is a complex of 13 different subunits. We present the full ami... more The mammalian cytochrome c oxidase is a complex of 13 different subunits. We present the full amino acid sequence of the one remaining uncharacterized subunit, subunit IHQ in the nomenclature used here, VIIb in the numbering system of Kadenbach and colleagues (e.g. Kadenbach, B., and Merle, P. (1981) FEBS Lett. 135, 1-11). A partial protein sequence was obtained from the purified subunit isolated by gel filtration procedures. This information was used to synthesize an oligonucleotide probe which was then used to isolate a cDNA clone encoding the subunit. This cDNA for subunit IHQ is 480 base pairs long and encodes a polypeptide which is either 83 or 88 amino acids long, including an N-terminal leader sequence of either 27 or 32 residues. The molecular weight of the mature subunit IHQ is 6350 based on the amino acid sequence deduced from the gene. The leader sequence is typical of other mitochondrial target sequences in having several positively charged residues but no negatively charged side chains.
British Journal of Biomedical Science, 2008
This study aims to clarify the molecular characteristics of the urease gene operon from urease-po... more This study aims to clarify the molecular characteristics of the urease gene operon from urease-positive thermophilic campylobacters (UPTC) obtained from different sources and in various countries. Sequence heterogeneity was observed for the promoter structures at the -35-like region among the 12 isolates examined. The most probable TTG start codon was suggested for the ureB and ureH genes, and for the ureA, E, F and G genes, ATG was suggested among all the isolates examined. Overlap was detected between ureA and ureB and between ureB and ureE among all the isolates examined. UPTC is the first example of an overlap between the two structural genes ureA and ureB. When the completely sequenced open reading frames (ORFs) for ureE, ureF, ureG and ureH were identified, non-coding regions between ureE and ureF, ureF and ureG, and ureG and ureH were also demonstrated. All six start codons of the six urease genes were demonstrated to be preceded by Shine-Dalgarno sequences among all the isolates examined. The Cys-His sequence corresponding to urease active sites were aligned perfectly and fully conserved among the three UPTC isolates examined. A putative and intrinsic p-independent transcriptional terminator was identified to be identical among all the isolates examined. A partial and putative ORF of about 200 bp in length showing high sequence similarity to GTP cyclohydrolase I was observed downstream of ureH.
The Journal of Biochemistry, 1978
The chemical and catalytic properties of potato phosphoglucomutase [EC 2.7.5.1] were studied usin... more The chemical and catalytic properties of potato phosphoglucomutase [EC 2.7.5.1] were studied using various enzyme species (Peaks Ia, Ib, Ic, and II; Takamiya, S. & Fukui, T. (1978) Plant Cell Physiol. 19, 319--328). The molecular weights of the species are all approximately 60,000. No indication of the presence of subunit structure was obtained under various conditions. The amino acid composition of Peak Ia is generally similar to those of the enzymes from other sources, though it has some peculiarities. The Peak Ia and Peak II enzymes both absolutely require alpha-D-glucose 1,6-bisphosphate and Mg2+ for activity, and appear to have a "ping-pong" mechanism. A low concentration of Be2+ inhibits their action, the inhibition being retarded either by Mg2 or EDTA. Although the inhibition patterns by various metabolites, are similar for Peaks Ia and II, they differ in their kinetic parameters and optimal pH values.
Plant and Cell Physiology, 1978
Parasitology International, 2016
A method for purifying active liquid-cultured Caenorhabditis elegans using a modified Baermann ap... more A method for purifying active liquid-cultured Caenorhabditis elegans using a modified Baermann apparatus is 15 described. This method consists of two parts: 1) large-scale cultivation of C. elegans in liquid medium and 2) prep-16 aration of active nematodes without contamination using the Baermann apparatus.
Cytochrome Systems, 1987
The phosphoenolpyruvate carboxykinase (PEPCK)-succinate pathway is important in the energy metabo... more The phosphoenolpyruvate carboxykinase (PEPCK)-succinate pathway is important in the energy metabolism of anaerobic organisms such as Ascaris suum, which resides in the host’s small intestine where oxygen is fairly limited (1). The last step of this pathway is catalized by fumarate reductase activity of mitochondria, and our studies on the respiratory chain of mitochondria from Ascaris indicated that complex II (succinate-ubiquinone reductase) functions as fumarate reductase by its reverse reaction. Complex II contains b-type cytochrome and properties of this cytochrome of Ascaris was somewhat different from that of beef heart. Comparative characterization of these b cytochromes are described.