Shohreh Alipour - Academia.edu (original) (raw)

Papers by Shohreh Alipour

Research in Pharmaceutical Sciences, Aug 30, 2012

Background and Aims: There has been considerable interest in developing magnetic nanoparticles as... more Background and Aims: There has been considerable interest in developing magnetic nanoparticles as localized drug delivery carriers. Paclitaxel (PTX) is an effective chemotherapeutic agent which is used for various cancers. PTX is a substrate of cell efflux pumps and some tumors show resistance to PTX due to multi drug resistance (MDR) phenotype. Verapamil is a calcium channel blocker able to reverse the resistance caused by Pglycoprotein (P-gp). Development of a drug delivery system capable of co-administration of paclitaxel and a Pgp inhibitor to cancer cells would be valuable for overcoming drug resistance. Methods: PLGA-magnetic nanoparticles (MNP) were prepared using magnetic nanoparticles and PLGA for the purpose of magnetic drug delivery. Magnetic nanoparticles of magnetite were prepared by chemical coprecipitation of ferric and ferrous chloride salts in a strong basic solution (ammonium hydroxide). PLGA-MNP containing paclitaxel and verapamil were prepared by a modified O/W emulsion solvent evaporation technique. The morphology, size, drug loading efficiency and drug release from the particles were investigated. Cell culture studies in resistant and susceptible cell lines are under development. Results: Magnetic nanoparticles before encapsulation in PLGA particles were less than 100 nm in size. All MNPs were almost spherical in shape and were found to have a mean diameter within the range of 400-800 nm. Drug encapsulation efficiency of magnetic-nanoparticles was 63% for 2min sonication. MNPs were responsive to external magnetic field. Conclusions: Magnetic-PLGA nanoparticles developed in this study may be used as a potential delivery system for reversion of MDR in cells resistant to PTX.

Journal of Drug Delivery Science and Technology, Feb 1, 2022

DOAJ (DOAJ: Directory of Open Access Journals), Mar 1, 2015

Ondansetron hydrochloride, a selective 5-HT3 receptor blocker, is an effective antiemetic drug wi... more Ondansetron hydrochloride, a selective 5-HT3 receptor blocker, is an effective antiemetic drug with oral bioavailability of %60 and half-life of 4-5 h. The present study was carried out to prepare fast dissolving films of ondansetron hydrochloride to increase patient compliance and improve efficacy of drug. Films were prepared by solvent casting method, using poly vinyl alcohol, poly vinyl pyrrolidone and konjacglucomannan as film formers and PEG400 as plasticizer. Natural and synthetic sweeteners were used for masking bitterness of drug. Satisfactory results were obtained from evaluation of physical characteristics of fast dissolving films of ondansetron hydrochloride including: thickness: 37-39mm, surface pH: 6.77, folding endurance: up to 300 time and tensile strength: 35.75-50.93g/cm². Films were also subjected to an in vitro dissolution and release studies. In vitro drug release studies indicated 93-95% release in 5 min. Fast dissolving films of ondansetron could be a potential alternative for the currently marketed oral formulation, parenteral form and suppository with better patient compliance and higher bioavailability for the rapid control of emesis.

Colloids and Surfaces B: Biointerfaces, Dec 1, 2010

In primary or metastatic lung cancer, administration of chemotherapeutic agents via inhalation co... more In primary or metastatic lung cancer, administration of chemotherapeutic agents via inhalation could increase exposure of lung tumor to the drug, while minimizing systemic side effects. In addition, mucosal drug delivery system may reduce the toxicity and increase the site specific efficacy of drugs. Sodium alginate is a biodegradable, biocompatible and mucoadhesive polymer which is used for site specific drug delivery to mucosal tissues. At the present study alginate microparticles were fabricated by an emulsification technique and characterized. Selection of appropriate parameters enabled the preparation of microparticles with a mean volume diameter of 3±0.7 μm, mass median aerodynamic diameter of 5.9±0.33 μm, fine particle fraction of 13.9±0.57% and encapsulation efficiency of 61±4%. The in vitro release profile showed a slower release rate for microparticles compare to pure paclitaxel. Physiochemical characterization of paclitaxel via FT-IR, DSC and XRD techniques revealed information of solid state of paclitaxel loaded microparticles. The in vitro cytotoxicity activity of paclitaxel loaded microparticles was assessed using human non-small cell lung cancer cell lines (A549 and Calu-6). Results showed that exposure of cells to pure paclitaxel and paclitaxel loaded microparticles effectively inhibited the growth of A549 and Calu-6 cells similarly in a concentration-and time-dependent manner.

Journal of Cosmetic Dermatology

Drug Delivery and Translational Research, Oct 19, 2022

The recent outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China has spread rapidly aro... more The recent outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China has spread rapidly around the world, leading to a widespread and urgent effort to develop and use comprehensive approaches in the treatment of COVID-19. While oral therapy is accepted as an effective and simple method, since the primary site of infection and disease progression of COVID-19 is mainly through the lungs, inhaled drug delivery directly to the lungs may be the most appropriate route of administration. To prevent or treat primary SARS-CoV-2 infections, it is essential to target the virus port of entry in the respiratory tract and airway epithelium, which requires rapid and high-intensity inhibition or control of viral entry or replication. To achieve success in this field, inhalation therapy is the most attractive treatment approach due to efficacy/ safety profiles. In this review article, pulmonary drug delivery as a unique treatment option in lung diseases will be briefly reviewed. Then, possible inhalation therapies for the treatment of symptoms of COVID-19 will be discussed and the results of clinical trials will be presented. By pulmonary delivery of the currently approved drugs for COVID-19, efficacy of the treatment would be improved along with reducing systemic side effects.

Frontiers in Nutrition

BackgroundThe co-occurrence of obesity and mood impairments named as “metabolic mood syndrome” (M... more BackgroundThe co-occurrence of obesity and mood impairments named as “metabolic mood syndrome” (MMS) is often neglected in the obesity management. This study aimed to evaluate effects of Probio-Tec ®BG-VCap-6.5 and magnesium co-supplementation on mood, cognition, intestinal barrier function and serum C reactive protein (CRP) levels in participants with obesity and depressed mood.DesignSeventy-four eligible participants were randomly allocated to either Probio-Tec®BG-VCap-6.5 [containing Lactobacillus rhamnosus (LGG®) and Bifidobacterium animalis subsp. Lactis (BB-12®)] + Magnesium chloride or placebo for 9 weeks. Sociodemographic data were collected in the beginning. Anthropometric, dietary and physical activity (PA) assessments were carried out. Beck Depression Inventory-II (BDI-II) and Montreal Cognitive Assessment (MoCA) scores were assessed through validated questionnaires. Fasting plasma zonulin, lipopolysaccharide (LPS) and (CRP) were measured by ELIZA kits.ResultsOf seventy-f...

Nutrition & Food Science

Purpose The purpose of this study was to evaluate the effects of methyl donor supplementation on ... more Purpose The purpose of this study was to evaluate the effects of methyl donor supplementation on anthropometric indices, body composition, glycemic control, lipid profile, homocysteine and appetite regulatory hormones in overweight and obese subjects. Design/methodology/approach This randomized double-blind placebo-controlled trial was conducted on overweight and obese subjects in Shiraz, Iran, in 2018. The 25 participants in each intervention and control group were required to take methyl donor supplement and placebo, respectively, for eight weeks. A variety of measurements was collected at baseline and the end of the study. The data were analyzed using SPSS 21 software. Findings The results indicated a significant reduction in weight, body mass index, body fat and waist and hip circumference (p < 0.001 for all parameters) within both groups and a significant improvement in skeletal muscle (p = 0.005), fat-free mass (p = 0.006), visceral fat area (p < 0.001) and body cell mas...

Frontiers in Materials

Among different topical drug delivery systems, nanoemulsions (NEs) are attractive due to enhanced... more Among different topical drug delivery systems, nanoemulsions (NEs) are attractive due to enhanced skin permeation potential and minimum toxicity. Topical nanoemulgels (NEMGELs) contain both gelling agents and NEs and thus can enhance skin permeation with sustained effects at the site of application. Oxybutynin (OXB) is an anti-muscarinic agent that is administered orally to treat hyperhidrosis but its systemic side effects may decrease patient compliance. Therefore, it assumes that oxybutynin-nanoemulgel (OXB-NEMGEL) with sustained release properties and skin permeation ability may be a suitable alternative. Using Design Expert software®, 17 OXB-NE formulations were prepared and optimized based on particle size, zeta potential and physical stability. Three polymers were used to prepare and optimized OXB-NEMGEL based on spreadability and viscosity. In-vitro drug release and ex-vivo skin permeation were investigated for optimized formulation. The model suggested by Design-Expert was q...

Iranian Journal of Basic Medical Sciences, 2021

Objective(s): Ocular inserts are usually polymeric thin films with increased ocular residence tim... more Objective(s): Ocular inserts are usually polymeric thin films with increased ocular residence time and sustained drug release capacity. Sodium alginate is a biocompatible and biodegradable carrier; however, initial burst release of encapsulated drug within it, is recognized as a challenge. Grafting –addition of functional moieties to a polymer– is a technique to modify polymers’ physicochemical properties, including higher ability to control drug release. Linezolid (LNZ) solution is used in consecutive doses in treatment of antibiotic-resistant Gram-positive bacterial infections especially induced by methicillin resistant Staphylococcus aureus (MRSA). Materials and Methods: Grafted alginate copolymers were synthesized using butyl methacrylate (BMC) and lauryl methacrylate (LMC) at two different reaction times (12 hr and 24 hr). Copolymerization was evaluated by 1H-NMR, Ft-IR, and TGA. Copolymer safety was examined by cytotoxicity test against HEK-293 cell. Linezolid inserts were pre...

Research in Pharmaceutical Sciences, Jan 9, 2012

Iranian Red Crescent Medical Journal, 2016

Background: Insulin resistance is a measure of metabolic stress in the perioperative period. Befo... more Background: Insulin resistance is a measure of metabolic stress in the perioperative period. Before now, no clinical trial has determined the summative effects of glutamine, L-carnitine, and antioxidants as metabolic conditioning supplements in the perioperative period. Objectives: The purpose of this study was to determine the effects of a new conditioning supplement on perioperative metabolic stress and clinical outcomes in non-diabetic patients. Patients and Methods: In this randomized controlled trial, 89 non-diabetic patients scheduled for coronary artery bypass grafting, with ejection fractions above 30%, were selected. Using the balanced block randomization method, the patients were allocated to one of four study arms: 1) SP (supplement/placebo): supplement seven days before and placebo 30 days after the surgery; 2) PS: placebo before and supplement after the surgery; 3) SS: supplement before and after the surgery; and 4) PP: placebo before and after the surgery. The supplement was composed of glutamine, L-carnitine, vitamin C, vitamin E, and selenium, which was manufactured for the first time by this research team. Five blood samples were drawn: seven days preoperatively, at the entrance to the operating room, while leaving the operating room, seven days postoperatively, and 30 days postoperatively. Levels of glucose, insulin, and HbA1c were measured in blood samples. Insulin resistance and sensitivity were calculated using a formula. Surgical complications were assessed 30 days postoperatively. Data analysis was done using one-way ANOVA, the Chi-square test, and a general linear model repeated-measures analysis with Bonferroni adjustment. Results: Blood glucose levels were increased postoperatively in the four groups (< 0.001), but a significantly higher increase occurred in the PP group compared to the SP (0.027), PS (0.026), and SS (0.004) groups. The superficial wound infection rate was significantly different between the four groups (0.021): 26.08% in PP, 9.09% in SP, 4.54% in PS, and 0% in SS. Conclusions: Our new metabolic conditioning supplement, whether given pre-or postoperatively, led to better perioperative glycemic control and decreased postsurgical wound infections in non-diabetic patients.

European Journal of Ophthalmology, 2009

Purpose To determine whether topical tranexamic acid can provide acceptable therapeutic concentra... more Purpose To determine whether topical tranexamic acid can provide acceptable therapeutic concentration in the aqueous humor. Methods A total of 47 patients with cataract as the sole eye disease who have been scheduled for cataract operation received one drop of tranexamic acid 5% solution at various time intervals prior to operation (from 15 minutes to 9 hours). Two patients received 10% solution of the drug. Paracentesis of the anterior chamber was done and the aqueous humor was aspirated in the operation room. The aqueous samples were analyzed using high-performance liquid chromatography method with the limit of quantification around 0.1 μg/mL. Systemic and ocular side effects were evaluated. Results Aqueous concentrations of tranexamic acid was higher than 1.5 μg/mL up to 160 minutes after administration of a single drop of 5% solution of the drug and then declined to an average concentration of 1 μg/mL at 300 minutes which persisted up to 9 hours after administration. In two pati...

Journal of Microencapsulation, 2015

Large porous particles (LPPs) could be used as a useful carrier for non-invasive delivery to the ... more Large porous particles (LPPs) could be used as a useful carrier for non-invasive delivery to the deep lung. Pulmonary delivery of paclitaxel-loaded LPPs (PTX-LPPs) can help to eliminate the highly complicated and harmful solvent used in PTX parenteral formulations. PTX-LPPs with mass median aerodynamic diameter (MMAD) of 5.74 ± 0.09 μm, high encapsulation efficiency and good aerosolisation properties were produced using ammonium bicarbonate as porogen. Cytotoxicity of PTX-LPPs on A549 and Calu-6 cell lines was comparable with Free-PTX. Endotracheal administration of PTX-LPPs in rats exhibited PTX plasma concentration in the therapeutic range which lasted 4-fold longer than i.v. injection. The bioavailability was measured as 51 ± 7.1%. The lung targeting efficiency (Te) of PTX-LPPs was 11.9-fold higher than i.v. administration. PTX-LPPs could deliver a higher PTX to lung with a non-toxic plasma level in a longer duration which shows their pulmonary delivery suitability.

Journal of Drug Delivery Science and Technology

Research in Pharmaceutical Sciences, 2012

Background and Aims:Magnetic nanoparticles (MNPs) are of high interest due to their application i... more Background and Aims:Magnetic nanoparticles (MNPs) are of high interest due to their application in medical fields, in particular for theranostics. Various coatings were studied to overcome the intrinsic instability of magnetite particles (Fe3O4) over long periods of time and their susceptibility to the oxidization in air. Encapsulating MNPs in polymeric microparticles would overcome the rapid clearance of Methotrexate from targeted organs. The objective of this study is to prepare and characterize these dual characteristics carriers suitable for regional drug delivery. Methods:MNPs were synthesized by co-precipitation method. The effect of coating materials of oleic acid, citric acid, polyvinyl alcohol and carboxymethyl cellulose on particles morphology, stability, paramagnetic effect and cytotoxicity were evaluated. Methotrexate loaded PLGA microparticles containing selected MNPs were prepared using double emulsion-solvent evaporation method in which the effects of drug to polymer ratio, PVA concentration and stirring speed were studied. Size, drug and magnetite encapsulation efficiency, surface morphology, magnetization saturation, in vitro release and cell toxicity of selected formulations were evaluated. Results:The optimized MNPs were effectively encapsulated in methotrexate loaded PLGA particles. By evaluating the affective parameters, 0.4-30µm size range particles was produced. Approximately, 94.57% drug encapsulation efficiency was resulted by further optimization. These particles have magnetization saturation of about 43emu/g with no remanence time. Methotrexate released in the range of 13.36% to 88.12% within 76 h. Cell toxicity results support release profile of invested particles. Conclusions:In general, the designed system is suitable for magnetically guided drug targeting and interesting carrier for regional Methotrexate drug delivery due to its appropriate particle size distribution, high magnetic saturation, sufficient drug loading, controlled drug release and low cell toxicity.