Shraddha Sahu - Academia.edu (original) (raw)

Papers by Shraddha Sahu

International Research Journal of Ayurveda & Yoga, Jun 10, 2020

International Research Journal of Ayurveda & Yoga, Jun 3, 2020

International journal of scientific research, 2020

Background : TB remains a major health concern world wide with extrapulmonary TB accounting for 2... more Background : TB remains a major health concern world wide with extrapulmonary TB accounting for 20% cases in India. Tubercular lymphadenopathy is most common extrapulmonary manifestation in TB. Aim: To compare and analyse cytomorphology patterns, AFB positivity and CBNAAT for detection of tubercular lymphadenopathy Methodology: a total of 80 FNAC samples from lymph nodes were taken, one part was sent for CBNAAT and other part was used to prepare slides and stained with H& E, PAP, MGG, ZN stain for AFB. Results:Out of 80 cases, 52 (65%)were granulomatous, 14 (17.5%)each were of reactive and suppurative. Among these 52 cases reported as granulomatous 32 were positive for CBNAAT, 11 AFB positive . Only 1 case out of 14 reactive cases was positive for CBNAAT. Out of 14 suppurative cases, 4 cases were CBNAAT positive. Conclusion: CBNAAT is a rapid test, can detect even if bacilli is less in number but FNAC along with AFB is simple procedure in resource limited area.

International Journal of Agricultural Engineering, 2018

Desiccant cooling system is one of the options in our daily life to provide the best indoor air q... more Desiccant cooling system is one of the options in our daily life to provide the best indoor air quality and thermal comfort with the minimum consumption of energy. In cooling systems a solid desiccant or a liquid desiccant solution can be used to assist in the cooling process. Desiccant has a property of regeneration so it can be recirculated and reused. In this cooling system we use liquid CO 2 /dry ice to achieve the sensible heat reduction while using silica gel(desiccant) to absorb moisture and decrease latent heat.The desiccant systems are reasonablypriced, produce no CFCs, and capable of both drying and filtering the air. They provide an opportunity to control humidity and temperature independently, and have the capability of using low quality thermal energy. The performance of the system is evaluated using Cooling Capacity (CC) parameter. The system under a typical summer day of hot and humid climate was tested. A remarkable decrease about 40-65% in the specific humidity and with a supply air temperature lower than 25°C of the proposed system was observed. The study is important and helpful to improve the effectiveness of this kind of liquid desiccant system in hot and humid places.

British Journal of Haematology, 2020

A 13-year-old boy was referred to our haematology department with hyperthermia, headaches, confus... more A 13-year-old boy was referred to our haematology department with hyperthermia, headaches, confusion and vomiting, associated with hepatomegaly and haemorrhagic features (epistaxis and conjunctival haemorrhages). A full blood count showed pancytopenia: leucocyte count of 2 2 9 10/l, haemoglobin concentration of 100 g/l, platelet count of 28 9 10/l and reticulocyte count of 21 9 10/l. The peripheral blood film showed atypical lymphocytes and revealed ring forms of Plasmodium falciparum (left) with 2 5% parasitaemia. A diagnosis of airport malaria was made, as the patient had not travelled recently and lived near a military airport. He was treated with intravenous artenusate. Due to the persistence of cytopenias, bone marrow aspiration was performed and showed increased macrophages with haemophagocytosis of leucocytes and the presence of haemozoin in the cytoplasm (right). He fulfilled other macrophage activation syndrome (MAS) criteria, specifically a markedly elevated ferritin (11840 μg/l) and hypertriglyceridaemia (3 88 g/l). By day 3, parasites were no longer detectable and the cytopenias and features of MAS started to resolve without the need for corticosteroid treatment. One platelet transfusion was needed. Treatment was continued with artemether and lumefantrine. Macrophage activation syndrome/haemophagocytic lymphohistiocytosis is a hyperinflammatory condition with a high mortality rate. It can occur as a primary syndrome or more commonly secondary to an autoimmune, neoplastic or infection process, the latter including Epstein–Barr virus infection and also malaria. The pathogenetic mechanism proposed is an imbalance of proand anti-inflammatory cytokines leading to inappropriate immune stimulation and multiorgan dysfunction as a result of the hyperinflammatory response. The clinicopathological features of MAS and malaria can overlap. Clinicians therefore need to actively investigate for MAS when there is persistent fever or cytopenias despite appropriate anti-malarial therapy.

Radiotherapy and Oncology, 2018

Biomedicine & Pharmacotherapy, 2004

The goal was to use an artificial neural network model to predict the plasma concentration of ami... more The goal was to use an artificial neural network model to predict the plasma concentration of aminoglycosides in burn patients and identify patients whose plasma antibiotic concentration would be sub-therapeutic based on the patients' physiological data and taking into account burn severity. Physiological data and some indicators of burn severity were collected from 30 burn patients who received arbekacin. A three-layer artificial neural network with five neurons in the hidden layer was used to predict the plasma concentration of arbekacin. Linear modeling for prediction of plasma concentration and logistic regression modeling for the classification of patients were also used and the predictive performance was compared to results from the artificial neural network model. Dose, body mass index, serum creatinine concentration and amount of parenteral fluid were selected as covariates for the plasma concentration of arbekacin. Area of burn after skin graft was a good covariate for indicating burn severity. Predictive performance of the artificial neural network model including burn severity was much better than linear modeling and logistic regression analysis. An artificial neural network model should be helpful for the prediction of plasma concentration using patients' physiological data, and burn severity should be included for improved prediction in burn patients. Because the relationship between burn severity and plasma concentration of aminoglycosides is thought to be nonlinear, it is not surprising that the artificial neural network model showed better predictive performance compared to the linear or logistic regression models.

American Journal of Infectious Diseases, 2013

In order to understand the mechanism of molecular interactions at the active site of Tryparedoxin... more In order to understand the mechanism of molecular interactions at the active site of Tryparedoxin Peroxidase (Try P), homology modeling and docking studies were performed. We generated a Three-Dimensional (3D) model of target protein based on the Crystal structure of Leishmania Major Try PI (PDB ID: 3TUE) using modeler software. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (Try P). Inhibition of the Tryparedoxin peroxidase interaction has become a new therapeutic strategy in treating leishmaniasis. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (TryP). Tryparedoxin peroxidase of Trypanosomatidae family functions as antioxidant through their peroxidase and peroxynitrite reductase activities. The theoretical docking study, conducted on a sample previously reported for anti-cancer properties of Methotrexate at the binding site of 3D models of Tryparedoxin Peroxidase of Leishmania braziliensis (L. braziliensis Try P) examine interaction energy. Our studies indicate that Methotrexate displays potent activity against Try P with lowest binding energy and RMSD values to be-14.5879 Kcal/Mol and 2.0 A. The results of the present study clearly demonstrated the Tryparedoxin Peroxidase inhibitory activity by methotrexate in in silico docking analysis and in vitro assay which contributes towards understanding the mechanism of antileishmanial activity.

American Journal of Biochemistry and Biotechnology, 2013

A.niger Rnase was designed from ACTBIND (PDB ID: 3D3Z). Yeast actin-human gelsolin segment 1 comp... more A.niger Rnase was designed from ACTBIND (PDB ID: 3D3Z). Yeast actin-human gelsolin segment 1 complex (PDB ID: 1YAG) was used as template for L. donovani actin protein for 3D model in Modeller9v8. These models were testified by PROCHECK, ERRAT, WHAT-IF, PROSA2003 and VERIFY-3D. All evidences suggest that the geometric quality of the backbone conformation, energy profile, residue interaction and contact of the structures were well within the limits of reliable structures. The interaction energy of docking was calculated using the HEX server. Etotal and calculated RMSD values were-1.902,-9.323 kcal moL −1 and 0.402 Å, respectively. The study presented here has an advantage to design molecules that may have antileishmanial activity.

INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH, 2020

Background- The diagnostic detection of malignant cells in effusions serves as a mainstream diagn... more Background- The diagnostic detection of malignant cells in effusions serves as a mainstream diagnostic tool and a predictor of the spread of diseases. Aim-To compare diagnostic efficacy of liquid based cytology (LBC) with conventional cytopreparatory techniques of pleural, peritoneal and pericardial fluids. Methodology - 110 peritoneal, pleural and pericardial effusions were received from indoor of various departments, microscopic examination was carried out by conventional and SurePath LBC method. For each case scoring was done for cellularity, cell morphology, distribution and background. They were classified as Non-neoplastic, suspicious for malignancy and malignant. Results - LBC showed better cell yield, morphology, distribution and background. Non-neoplastic lesions were maximum in number and constituted 78 (70.9%) cases, 8 (7.3%) cases were suspicious for malignancy, 22 (20%) malignant cases and 2 cases were unsatisfactory for evaluation. Conclusion- compared to conventional ...

Journal of Evolution of medical and Dental Sciences, Apr 1, 2014

We report a 64 year old female who presented with epigastric pain and intractable vomiting not re... more We report a 64 year old female who presented with epigastric pain and intractable vomiting not respond to treatment and we were not able to pass nasogastric tube. Chest x-ray shows eventration of left sided hemi diaphragm, upper gastro intestinal contrast study conform the diagnosis of gastric volvulus. Plication of left hemidiaphragm with anterior gastropexy was performed through an abdominal approach. Postoperatively the patient's symptoms improved. Acute gastric volvulus carries a mortality rate of 42-56%, secondary to gastric ischemia, perforation or necrosis. 1 Emergency physicians should have suspicion about gastric volvulus when treating patients with abdominal pain and persistent vomiting. The patient should go for surgical consultation as early as possible.

American Journal of Biochemistry and Molecular Biology, 2013

homology modeling and inhibitory effects of flavonoids for anti-leishmanial activity

International Journal of Orthopaedics Sciences, 2018

Background: Osteoporosis is a major public health problem as it is associated with substantial mo... more Background: Osteoporosis is a major public health problem as it is associated with substantial morbidity and socioeconomic burden.

Biomaterial-associated infection is a significant cause of concern in health care and clinical fi... more Biomaterial-associated infection is a significant cause of concern in health care and clinical field. It is widely acknowledged that material with intended use in a medical application must not support colonization and attachment of bacteria on its surfaces. This has encouraged the search for the newer polymeric biomaterial with anti-adhesive and anti-adherence properties to resist bacterial attachment. Firsthand information about the combined effect of various material properties that affects bacterial attachment to biomaterial surface during the development of anti-adhesive material is always useful. In this work, computer-aided techniques, i.e., the neural network was applied to build a model using biomaterial properties such as surface roughness, swelling ratio, and pore size as input and number of surface adhered bacterial cells as output response. Various blending combinations of silk fibroin and xanthan were used to generate biocomposites with varying surface roughnesses. The...

DOI: 10.21276/sjams.2018.6.9.50 Abstract:Worldwide 1 in 3 women over 50 years of age experience a... more DOI: 10.21276/sjams.2018.6.9.50 Abstract:Worldwide 1 in 3 women over 50 years of age experience an osteoporotic fracture. Measuring bone mineral density is the most important tool in the diagnosis osteoporosis.To compare number of reproductive years, BMI and natural and surgical menopause with bone mineral density in post-menopausal women.Two hundred postmenopausal women were studied at the Department of Obstetrics &Gynaecology, King Georges Medical University, Lucknow from September 2015 to August 2016. A detailed questionnaire was filled which included details of demographic factors of the patient, BMI, Blood pressure, number of reproductive years, whether menopause was natural or surgical and personal history including history of exercise. BMD was measured in each woman at lumbar spine (L1-L4) by lunar Prodigy dual energy X Ray absorptiometry. Mean BMI showed a significant declining trend from normal to osteoporosis BMD status (p<0.001). Among overweight and obese patients maj...

New Biotechnology, 2018

Aqueous two-phase system (ATPS) is suitable for purification of enzymes and proteins by preferent... more Aqueous two-phase system (ATPS) is suitable for purification of enzymes and proteins by preferentially partitioning the desired biomolecule in one phase and interfering substances into another phase. The biocompatibility of ATPS provides a very low interfacial tension between the phases, which results in high mass transfer and ease of scale up. Bromelain is the denomination given to the group of endoproteases obtained from members of the Bromeliaceae family. The objective of the present study is to relate the main studies in India that has proven that bromelain purification can be cost-effective, in addition to the well-known benefits owned by such enzymes, and highlight the applications that create their market potential in the World. In the present work, partitioning of bromelain enzyme, extracted from the peel and crown leaves of pineapple fruit, in different PEG/salt two-phase systems was studied. ATPS comprising different compositions of PEG X (X = 4000, 6000)/salts (ammonium sulfate, sodium sulfate, potassium phosphate and trisodium citrate) were formed and characterized with the help of binodal curve and tie-line length. The specific activity of Bromelain in the crude extract of peel and crown was 0.15 U/mg and 0.25 U/mg respectively, and Specific activity after purification using ammonium sulfate precipitation, dialysis and ATPS (PEG 4000-Ammonium Sulfate-Water system) was 1.66 U/mg and 12 U/mg for peel and Crown respectively. Final fold purification of peel and crown is 11.06 and 48 respectively.