Shree Joshi - Academia.edu (original) (raw)

Papers by Shree Joshi

International Journal of Epidemiology

Background In comparison to the slow progression of first wave of the COVID-19 pandemic, by March... more Background In comparison to the slow progression of first wave of the COVID-19 pandemic, by March 2021, the second wave appeared to be much more aggressive with many more cases. We aimed to evaluate reported COVID-19 epidemiology data to better understand the pandemic's progression in Rural Bikaner. Methods A record-based descriptive analysis study between first and second covid-19 waves, on the variables of interest including positivity rates, case fatality rate, demographic profile of positive patients. Results the daily reported cases at the peak of the first wave in rural area in 2020 was 20-25 cases per day and in second wave number of cases was increased double to first wave. Death rate and severity was also increased in second wave. In second wave the mean age of positive patients was decreased. Conclusions In our analysis rural area had a more severe second wave of the COVID-19 pandemic than the first and highlights the importance of examining multiple epidemiological va...

Indian Journal of Community Health

Background: Knowledge of Community Medicine plays a pivot role in effective delivery of health ca... more Background: Knowledge of Community Medicine plays a pivot role in effective delivery of health care services. Effective Teaching-Learning (T-L) of a class of 150-200 students is a challenge. Research is needed to identify methods suitable to the needs of the students. Students’ perspective is an important diagnostic feedback tool in this context. Aims & Objectives: To know the students’ preferences and perceptions regarding current T-L methods and their felt need for modification in the T-L approach. Material & Methods: Responses were collected from students pursuing internship regarding their preferences and perception on a pretested, validated semi structured questionnaire, most of the responses were collected on a Likert type five point scale. The data was analyzed by calculating percentage, mean and Standard Deviation (SD). Result: Majority (66.2%) felt a need for modification in T-L Approach and 74% of these opined that it will improve their performance in assessments. Presenta...

Indian Scientific Journal Of Research In Engineering And Management, Apr 6, 2022

The study was conducted in order to know Indian users' point of view on impact of Chinese apps ba... more The study was conducted in order to know Indian users' point of view on impact of Chinese apps ban on Indian markets. We have taken, primary data collected from Indians and secondary data collected from experts' articles as our source. We studied about various parameters of Indian market like employment, investments, time-usage, preferences, inclination of support of users to use Indian apps and thus the potential in these markets, incentives provided by government and certain start-ups, overall impact on Chinese and Indian markets and economy and we concluded our research paper based on the findings.

Lecture Notes in Electrical Engineering, 2019

E-learning is one of the best tools to support an individual’s education system worldwide. There ... more E-learning is one of the best tools to support an individual’s education system worldwide. There is a demand to create an e-learning site in Kannada for disabled people to remove accessibility barriers. In this paper, we have introduced a new framework for Web accessibility, providing easy access and lifelong learning to the Web site for Kannada’s deaf/blind speakers. The main purpose of this framework is to support Kannada’s deaf/blind by using the language of Kannada sign, speech text, and Kannada Moon code. Kannada-speaking disabilities can learn effectively in this framework.

This document proposes an optional mechanism as part of IPv6 Stateless Address Autoconfiguration ... more This document proposes an optional mechanism as part of IPv6 Stateless Address Autoconfiguration for distribution of unique interface identifiers to IPv6 hosts on a link. Hosts can then use these unique interface identifiers to generate unique autoconfigured link local and global unicast addresses. This mechanism is intended for use in networks where link layer identifiers are used for generating interface identifiers and where non unique link layer identifiers will result in duplicate link local addresses. An example of such network is Ethernet Broadband access networks.

International Journal of Medical and Biomedical Studies, 2020

Background: A mass in the right iliac fossa is a common diagnostic problem encountered in clinica... more Background: A mass in the right iliac fossa is a common diagnostic problem encountered in clinical practice, requiring skill in diagnosis. Methods: 100 patients with signs and symptoms of right iliac fossa mass admitted in Hospital were identified and were studied by taking detailed clinical history, physical examination and were subjected to various investigations like x ray erect abdomen, chest x-ray, contrast x-ray . Result: In this study of out of 100 cases, 65.00% of cases were related to appendicular pathology either in the form of appendicular mass or appendicular abscess. There were 12.00% cases of ileocaecal tuberculosis. Conclusion: Appendicular lump remains the most common cause for right iliac fossa pain. Ileocaecal tuberculosis is one of the most important differential diagnoses for pain abdomen. Keywords: Appendicular Mass, Ileocaecal Tuberculosis, Carcinoma Caecum, Right Iliac Fossa Mass.

Oncotarget, Jan 25, 2018

Multiple myeloma has a continued need for more effective and durable therapies. B cell maturation... more Multiple myeloma has a continued need for more effective and durable therapies. B cell maturation antigen (BCMA), a plasma cell surface antigen and member of the tumor necrosis factor (TNF) receptor superfamily, is an attractive target for immunotherapy of multiple myeloma due to its high prevalence on malignant plasma cells. The current work details the pre-clinical evaluation of BCMA expression and development of a chimeric antigen receptor (CAR) targeting this antigen using a fully human single chain variable fragment (scFv). We demonstrate that BCMA is prevalently, but variably expressed by all MM with expression on 25-100% of malignant plasma cells. Extensive Immunohistochemical analysis of normal tissue expression using commercially available polyclonal antibodies demonstrated expression within B-lineage cells across a number of tissues as expected. Based upon the highly restricted expression of BCMA within normal tissues, we generated a set of novel, fully human scFv binding ...

Journal of Biomedical Nanotechnology, 2015

Mesothelin, a cancer biomarker overexpressed in tumors of epithelial origin, is a target for nano... more Mesothelin, a cancer biomarker overexpressed in tumors of epithelial origin, is a target for nanotechnology-based diagnostic, therapeutic, and prognostic applications. The currently available anti-mesothelin antibodies present limitations, including low penetration due to large size and/or lack of in vivo stability. Single domain antibodies (sdAbs) or nanobodies (Nbs) provide powerful solutions to these specific problems. We generated a phage-display library of Nbs that were amplified from B cells of a llama that was immunized with human recombinant mesothelin. Two nanobodies (Nb A1 and Nb C6) were selected on the basis of affinity (K D = 15 and 30 nM, respectively). Nb A1 was further modified by adding either a cysteine to permit maleimide-based bioconjugations or a sequence for the site-specific metabolic addition of a biotin in vivo. Both systems of conjugation (thiol-maleimide and streptavidin/biotin) were used to characterize and validate Nb A1 and to functionalize nanoparticles. We showed that anti-mesothelin Nb A1 could detect native and denatured mesothelin in various diagnostic applications, including flow cytometry, western blotting, immunofluorescence, and optical imaging. In conclusion, anti-mesothelin Nbs are novel, cost-effective, small, and single domain reagents with high affinity and specificity for the tumor-associated antigen mesothelin, which can be simply bioengineered for attachment to nanoparticles or modified surfaces using multiple bioconjugation strategies. These anti-mesothelin Nbs can be useful in both conventional and nanotechnology-based diagnostic, therapeutic and prognostic biomedical applications.

Journal of Biological Chemistry, 2005

We are interested in the mechanism of cyclooxygenase-2 (Cox-2) regulation in colon cancer cells b... more We are interested in the mechanism of cyclooxygenase-2 (Cox-2) regulation in colon cancer cells because this knowledge could provide insight into colon carcinogenesis and suggest ways to suppress Cox-2 expression in colon tumors. Studying the HT-29 colon cancer cell line as a model, we found that Cox-2 mRNA and protein levels were activated over 10-fold by the inflammatory cytokine tumor necrosis factor (TNF)-␣. Moreover, we found that the histone deacetylase inhibitors butyrate and trichostatin A could block Cox-2 activation in a gene-specific manner. TNF-␣ and butyrate did not significantly affect Cox-2 promoter activity, mRNA stability, or negative regulation by the Cox-2 3-untranslated RNA region. A nuclear run-on assay showed that TNF-␣ increased Cox-2 transcription, whereas butyrate was suppressive. Because butyrate has been reported to suppress polymerase elongation on the c-myc gene, we employed the chromatin immunoprecipitation assay to determine the influence of butyrate and trichostatin A on polymerase distribution on the Cox-2 gene. These data indicated that butyrate restricted polymerase elongation from exon 1 to 2 on both the c-myc and Cox-2 genes. We propose that histone deacetylases regulate a transcriptional block on the Cox-2 and c-myc genes and that this block may be a potential target for pharmacological intervention.

American Journal of Biochemistry and Biotechnology, 2013

Araucaria cookii, Bauhinia blakeana and Brassaia actinophylla are ornamental plants. The presence... more Araucaria cookii, Bauhinia blakeana and Brassaia actinophylla are ornamental plants. The presence of various phytochemicals and pharmacologically important compounds in these plants can be exploited for their medicinal use. But there are no reports on the phytochemical and pharmacological evaluation of these plants and this study aims at investigating these. The plant extracts were prepared in different solvents like methanol, ethanol, ethyl acetate, acetone, hexane, water and chloroform. Qualitative analysis of phytochemicals were assessed. The anti-microbial, anti-oxidant and enzyme inhibitory activity was determined for all three plant extracts. Anti-bacterial activity against three gram negative bacteria, E. coli, Pseudomonas and Klebsiella was done and Araucaria cooki showed highest antibacterial activity among the three plants. Maximum antioxidant activity was seen in methanol extract of Brassaia actinophyla with 81% inhibition. The order of the antioxidant activity of the three plants are in the order B.actinophylla>A cookie>B.blakeana. The results of phytochemical analysis suggest that phytosteroids are present in all the three plants. Maximum inhibition against the tested enzymes was exhibited by hexane and chloroform extracts of A.cookii. Hemolytic activity was done and the hexane extract showed maximum haemolysis where as aqueous extracts showed minimum activity. From the results it is clear that the three plant extracts has pharmacological applications. This is the first report of antimicrobial, antioxidant and enzyme inhibitory activities of these three plant extracts. Further studies are needed to exploit the actual mechanism and active compounds of these plants.