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Papers by Helder Da Silva

Research paper thumbnail of Hydrodynastes melanogigas Franco, Fernandes and Bentim, 2007 (Squamata: Serpentes: Colubridae): Range extension and new state record

Research paper thumbnail of A new two-pored species of Amphisbaena (Squamata, Amphisbaenidae) from the Brazilian Cerrado, with a key to the two-pored species of Amphisbaena

Zootaxa, Jan 3, 2016

A new species of Amphisbaena is described from municipalities of Babaçulândia, State of Tocantins... more A new species of Amphisbaena is described from municipalities of Babaçulândia, State of Tocantins, and Estreito, State of Maranhão, northern Brazilian Cerrado. The new species differs from other two-pored species of the genus, by presenting mainly slender body shape; snout rounded in profile and dorsal view; high number of body annuli (328-342); 12-14 dorsal segments and 14-16 ventral in midbody half-annulus; autotomic site between 9-10<sup>th</sup> caudal annuli; absence of chevron-shaped anterior dorsal half-annuli; 20-23 caudal annuli; postmalar row absent; and precloacals pores arranged in a continuous series of the precloacal half-annuli. Additionally, we present a key for two-pored species of Amphisbaena.

Research paper thumbnail of Uso do AHP na avaliçao do desempenho do sistema de gestao da qualidade, meio ambiente e segurança e saúde ocupacional integrados

Direccion Y Organizacion Revista De Direccion Organizacion Y Administracion De Empresas, 2011

Research paper thumbnail of Monthly analysis, seasonal and interanual the reference evapotranspiration for the southwestern Amazon, Acre, Brazil

Revista Brasileira de Geografia Física, 2015

Research paper thumbnail of Biocompatible Material, Process for Obtaining Said Biocompatible Material and Use of Said Biocompatible Material in Implants and Prostheses

Research paper thumbnail of Evaluation of Reference Genes for RT-qPCR Normalization in Cowpea under Drought Stress during Biological Nitrogen Fixation

Research paper thumbnail of Scinax Ruber. Predation. Natural History Notes

Herpetological Review

Between 2145 and 2210 h on 30 Dec 2009, we observed a Trachycephalus typhonius (94 mm SVL) preyin... more Between 2145 and 2210 h on 30 Dec 2009, we observed a Trachycephalus typhonius (94 mm SVL) preying on two Scinax ruber in the city of Carolina, Maranhão, northeastern Brazil.

Research paper thumbnail of <p class="HeadingRunIn"><strong>Redescription of <em>Phalotris labiomaculatus</em> (Serpentes, Dipsadidae, Elapomorphini), with notes on the taxonomic boundaries within the <em>nasutus</em> group</strong></p>

Zootaxa, 2013

was described based on a single specimen from Porto Franco, south of the Brazilian state of Maran... more was described based on a single specimen from Porto Franco, south of the Brazilian state of Maranhão, being rediscovered three years later in Mateiros, a municipality in the north of the state of Tocantins, attesting to its occurrence in the cerrado of both banks of the Tocantins River. The discovery of 28 new specimens from other localities during field expeditions (2009-2011) allowed a redescription of the species, adding new data on meristic and morpho-qualitative traits. These results enable a better diagnosis of intraspecific, ontogenetic and sexual variation, consolidating its taxonomic relationships with other species belonging to the nasutus group. The distribution map of P. labiomaculatus, as well as an identification key to the species of the natusus group, are provided. The type-locality is fixed to the municipality of Porto Franco, state of Maranhão.

Research paper thumbnail of Herpetofauna, Ponte de Pedra Hydroelectric Power Plant, states of Mato Grosso and Mato Grosso do Sul, Brazil

Research paper thumbnail of Hydrodynastes melanogigas Franco, Fernandes and Bentim, 2007 (Squamata: Serpentes: Colubridae): Range extension and new state record

Check List, 2012

Editorial responsibility: Pedro M. Sales Nunes

Research paper thumbnail of Estudo experimental das necroses cutâneas produzidas por fármacos utilizados na escleroterapia de teleangiectasias

Cir Vasc Angiol, Feb 1, 2000

Resumo: Com a finalidade de analisar as escaras produzidas pelos diferentes agentes nos procedime... more Resumo: Com a finalidade de analisar as escaras produzidas pelos diferentes agentes nos procedimentos escleroterápicos, foi realizado um estudo experimental em ambiente laboratorial. Usaram-se 20 ratos anestesiados submetidos à aplicaçäo uniforme de 0, 1ml ...

Research paper thumbnail of Automedicação entre graduandos de enfermagem, farmácia e odontologia da Universidade Federal de Alfenas

Revista Mineira De Enfermagem, Mar 1, 2007

Automedicação entre graduandos de enfermagem...

Research paper thumbnail of Abortamento: um estudo da morbidade hospitalar no país

Rbm Rev Bras Med, Apr 1, 2010

Research paper thumbnail of Algoritmo de Huffman aplicado à compactação de textos de jogos desenvolvidos pela ferramenta Dev X Game

Anais Sulcomp, Oct 3, 2015

Research paper thumbnail of Produção de Biomassa Seca e Pigmentos pela Microalga Neochloris oleoabundans em Diferentes Meios de Cultivo

Anais do V Simpósio de Bioquímica e Biotecnologia, 2015

Research paper thumbnail of 14697 - Efeitos da estiagem na produção familiar no sítio Guritiba, Queimadas-PB

Cadernos De Agroecologia, Dec 15, 2013

Research paper thumbnail of Development of microsatellite markers for Hyacinth macaw (Anodorhynchus hyacinthinus) and their cross-amplification in other parrot species

BMC Research Notes, 2015

Hyacinth macaw Anodorhynchus hyacinthinus is the largest parrot of the world and is considered vu... more Hyacinth macaw Anodorhynchus hyacinthinus is the largest parrot of the world and is considered vulnerable to extinction due to its habitat loss and illegal trade associated to the international pet market demand. Genetic studies on this species are still incipient to generate a consistent characterization of the population dynamics and to develop appropriate conservation strategies. In this sense, microsatellite markers may support the detection of a population genetic structure for this bird species. However, at this time, none Hyacinth macaw species-specific primers for microsatellite loci have been so far established. This study aimed to develop and characterize polymorphic microsatellite markers for A. hyacinthinus and to check for their cross-amplification in other parrot species. Sequences containing repeated dinucleotide motifs were prospected and optimized from a genomic library that was enriched for microsatellites using magnetic beads. The analyses of 43-57 samples from wild individuals of three distinct Brazilian subpopulations led to the characterization of five polymorphic microsatellite loci. Allele richness per locus ranged from two to 12. Three loci exhibited observed heterozygosity values higher than 50 %, but the overall average value among all loci was close to 45 %. In addition, successful primer cross-amplification was verified in seven other investigated species of Neotropical parrots. The newly developed markers have shown to be potentially useful for in situ and ex situ population studies to support future conservation actions of Hyacinth macaw and other parrots.

Research paper thumbnail of Acúmulo de óleo em sementes de soja cultivadas in vitro e in vivo

Semina: Ciências Agrárias, 2015

Research paper thumbnail of Molecularly imprinted sensor for voltammetric detection of norfloxacin

Sensors and Actuators B: Chemical, 2015

ABSTRACT

Research paper thumbnail of The Dengue risk transmission during the FIFA 2014 World Cup

Dengue is a viral infection that can produce a severe fever and symptoms that may require hospita... more Dengue is a viral infection that can produce a severe fever and symptoms that may require hospitalization. It is transmitted between humans by the urban-adapted, day-biting Aedes mosquitoes and is therefore a particular problem in towns and cities. To explore this risk, one has assessed the potential levels of exposure by a climate-driven model for dengue risk transmission in Brazil and records of its seasonal variation at the key sites. Like the weather, it is unworkable to forecast the precise situation with regard to dengue in Brazil in 2014. One can, however, make informed guesses on the basis of averaged records of dengue in previous years. For the areas around the World Cup stadiums, these records show that the main dengue season will have passed before the World Cup is held in June and July. Unfortunately, the risk remains, even this is low but not negliegible, during these months in the Brazilian north and northeast. But, in fact, the risk of an outbreak of dengue fever duri...

Research paper thumbnail of Hydrodynastes melanogigas Franco, Fernandes and Bentim, 2007 (Squamata: Serpentes: Colubridae): Range extension and new state record

Research paper thumbnail of A new two-pored species of Amphisbaena (Squamata, Amphisbaenidae) from the Brazilian Cerrado, with a key to the two-pored species of Amphisbaena

Zootaxa, Jan 3, 2016

A new species of Amphisbaena is described from municipalities of Babaçulândia, State of Tocantins... more A new species of Amphisbaena is described from municipalities of Babaçulândia, State of Tocantins, and Estreito, State of Maranhão, northern Brazilian Cerrado. The new species differs from other two-pored species of the genus, by presenting mainly slender body shape; snout rounded in profile and dorsal view; high number of body annuli (328-342); 12-14 dorsal segments and 14-16 ventral in midbody half-annulus; autotomic site between 9-10<sup>th</sup> caudal annuli; absence of chevron-shaped anterior dorsal half-annuli; 20-23 caudal annuli; postmalar row absent; and precloacals pores arranged in a continuous series of the precloacal half-annuli. Additionally, we present a key for two-pored species of Amphisbaena.

Research paper thumbnail of Uso do AHP na avaliçao do desempenho do sistema de gestao da qualidade, meio ambiente e segurança e saúde ocupacional integrados

Direccion Y Organizacion Revista De Direccion Organizacion Y Administracion De Empresas, 2011

Research paper thumbnail of Monthly analysis, seasonal and interanual the reference evapotranspiration for the southwestern Amazon, Acre, Brazil

Revista Brasileira de Geografia Física, 2015

Research paper thumbnail of Biocompatible Material, Process for Obtaining Said Biocompatible Material and Use of Said Biocompatible Material in Implants and Prostheses

Research paper thumbnail of Evaluation of Reference Genes for RT-qPCR Normalization in Cowpea under Drought Stress during Biological Nitrogen Fixation

Research paper thumbnail of Scinax Ruber. Predation. Natural History Notes

Herpetological Review

Between 2145 and 2210 h on 30 Dec 2009, we observed a Trachycephalus typhonius (94 mm SVL) preyin... more Between 2145 and 2210 h on 30 Dec 2009, we observed a Trachycephalus typhonius (94 mm SVL) preying on two Scinax ruber in the city of Carolina, Maranhão, northeastern Brazil.

Research paper thumbnail of <p class="HeadingRunIn"><strong>Redescription of <em>Phalotris labiomaculatus</em> (Serpentes, Dipsadidae, Elapomorphini), with notes on the taxonomic boundaries within the <em>nasutus</em> group</strong></p>

Zootaxa, 2013

was described based on a single specimen from Porto Franco, south of the Brazilian state of Maran... more was described based on a single specimen from Porto Franco, south of the Brazilian state of Maranhão, being rediscovered three years later in Mateiros, a municipality in the north of the state of Tocantins, attesting to its occurrence in the cerrado of both banks of the Tocantins River. The discovery of 28 new specimens from other localities during field expeditions (2009-2011) allowed a redescription of the species, adding new data on meristic and morpho-qualitative traits. These results enable a better diagnosis of intraspecific, ontogenetic and sexual variation, consolidating its taxonomic relationships with other species belonging to the nasutus group. The distribution map of P. labiomaculatus, as well as an identification key to the species of the natusus group, are provided. The type-locality is fixed to the municipality of Porto Franco, state of Maranhão.

Research paper thumbnail of Herpetofauna, Ponte de Pedra Hydroelectric Power Plant, states of Mato Grosso and Mato Grosso do Sul, Brazil

Research paper thumbnail of Hydrodynastes melanogigas Franco, Fernandes and Bentim, 2007 (Squamata: Serpentes: Colubridae): Range extension and new state record

Check List, 2012

Editorial responsibility: Pedro M. Sales Nunes

Research paper thumbnail of Estudo experimental das necroses cutâneas produzidas por fármacos utilizados na escleroterapia de teleangiectasias

Cir Vasc Angiol, Feb 1, 2000

Resumo: Com a finalidade de analisar as escaras produzidas pelos diferentes agentes nos procedime... more Resumo: Com a finalidade de analisar as escaras produzidas pelos diferentes agentes nos procedimentos escleroterápicos, foi realizado um estudo experimental em ambiente laboratorial. Usaram-se 20 ratos anestesiados submetidos à aplicaçäo uniforme de 0, 1ml ...

Research paper thumbnail of Automedicação entre graduandos de enfermagem, farmácia e odontologia da Universidade Federal de Alfenas

Revista Mineira De Enfermagem, Mar 1, 2007

Automedicação entre graduandos de enfermagem...

Research paper thumbnail of Abortamento: um estudo da morbidade hospitalar no país

Rbm Rev Bras Med, Apr 1, 2010

Research paper thumbnail of Algoritmo de Huffman aplicado à compactação de textos de jogos desenvolvidos pela ferramenta Dev X Game

Anais Sulcomp, Oct 3, 2015

Research paper thumbnail of Produção de Biomassa Seca e Pigmentos pela Microalga Neochloris oleoabundans em Diferentes Meios de Cultivo

Anais do V Simpósio de Bioquímica e Biotecnologia, 2015

Research paper thumbnail of 14697 - Efeitos da estiagem na produção familiar no sítio Guritiba, Queimadas-PB

Cadernos De Agroecologia, Dec 15, 2013

Research paper thumbnail of Development of microsatellite markers for Hyacinth macaw (Anodorhynchus hyacinthinus) and their cross-amplification in other parrot species

BMC Research Notes, 2015

Hyacinth macaw Anodorhynchus hyacinthinus is the largest parrot of the world and is considered vu... more Hyacinth macaw Anodorhynchus hyacinthinus is the largest parrot of the world and is considered vulnerable to extinction due to its habitat loss and illegal trade associated to the international pet market demand. Genetic studies on this species are still incipient to generate a consistent characterization of the population dynamics and to develop appropriate conservation strategies. In this sense, microsatellite markers may support the detection of a population genetic structure for this bird species. However, at this time, none Hyacinth macaw species-specific primers for microsatellite loci have been so far established. This study aimed to develop and characterize polymorphic microsatellite markers for A. hyacinthinus and to check for their cross-amplification in other parrot species. Sequences containing repeated dinucleotide motifs were prospected and optimized from a genomic library that was enriched for microsatellites using magnetic beads. The analyses of 43-57 samples from wild individuals of three distinct Brazilian subpopulations led to the characterization of five polymorphic microsatellite loci. Allele richness per locus ranged from two to 12. Three loci exhibited observed heterozygosity values higher than 50 %, but the overall average value among all loci was close to 45 %. In addition, successful primer cross-amplification was verified in seven other investigated species of Neotropical parrots. The newly developed markers have shown to be potentially useful for in situ and ex situ population studies to support future conservation actions of Hyacinth macaw and other parrots.

Research paper thumbnail of Acúmulo de óleo em sementes de soja cultivadas in vitro e in vivo

Semina: Ciências Agrárias, 2015

Research paper thumbnail of Molecularly imprinted sensor for voltammetric detection of norfloxacin

Sensors and Actuators B: Chemical, 2015

ABSTRACT

Research paper thumbnail of The Dengue risk transmission during the FIFA 2014 World Cup

Dengue is a viral infection that can produce a severe fever and symptoms that may require hospita... more Dengue is a viral infection that can produce a severe fever and symptoms that may require hospitalization. It is transmitted between humans by the urban-adapted, day-biting Aedes mosquitoes and is therefore a particular problem in towns and cities. To explore this risk, one has assessed the potential levels of exposure by a climate-driven model for dengue risk transmission in Brazil and records of its seasonal variation at the key sites. Like the weather, it is unworkable to forecast the precise situation with regard to dengue in Brazil in 2014. One can, however, make informed guesses on the basis of averaged records of dengue in previous years. For the areas around the World Cup stadiums, these records show that the main dengue season will have passed before the World Cup is held in June and July. Unfortunately, the risk remains, even this is low but not negliegible, during these months in the Brazilian north and northeast. But, in fact, the risk of an outbreak of dengue fever duri...