Silvia Luna - Academia.edu (original) (raw)

Papers by Silvia Luna

Research paper thumbnail of Extraction methods and nutritional characterization of protein concentrates obtained from bean, chickpea, and corn discard grains

Current Research in Food Science

Research paper thumbnail of Simple and efficient protocol for amaranth betalains extraction and stability analysis by ATR-FTIR spectroscopy

Journal of Cereal Science

Research paper thumbnail of Nutritional Characterization of Protein Concentrates Obtained from Bean, Chickpea, And Corn Discard Grains

Social Science Research Network, 2022

Research paper thumbnail of Desiccation-induced viable but nonculturable state in Pseudomonas putida KT2440, a survival strategy

PLOS ONE, 2019

S10 Fig. Amplifica6on of the16S rRNA gene from P. pu&da KT2440 by using the RT-PCR method. 1) Mar... more S10 Fig. Amplifica6on of the16S rRNA gene from P. pu&da KT2440 by using the RT-PCR method. 1) Marker 1 kb DNA Leader Jena Bioscience, 2) cells before desicca;on, 3) twenty-min rehydrated cells of 18 DABD. 4) Twenty four-hours rehydrated cells of 18 DABD, 5) twenty-min rehydrated cells of 40 DABD, 6) nega;ve control; reac;on without retrotranscriptase, and 7) nega;ve control; reac;on without template.

Research paper thumbnail of The phenological growth stages of different amaranth species grown in restricted spaces based in BBCH code

South African Journal of Botany, 2019

Amaranth is a pseudocereal with potential health benefits. Amaranth has recently gained importanc... more Amaranth is a pseudocereal with potential health benefits. Amaranth has recently gained importance due to its high capacity to grow in adverse conditions. Details about the growth and development of amaranth is fundamental to its cultivation, but reports on the phenological growth stages, development, and the life cycle of amaranth are limited. Under normal conditions, amaranth plants are as high as 2.2 m, making their handling difficult. Thus, this study determined the phenological growth stages and life cycle of amaranth in restricted spaces. Amaranthus cruentus, Amaranthus hybridus, and Amaranthus hypochondriacus plants were cultivated in restricted spaces. The physiological and qualitative features as number of leaves, length of plants and leaves, panicle color, were used to determine the different phenological growth stages and life cycle of amaranth plants. The phenological growth stages were described vía Biologische Bundesanstalt Bundessortenamt and CHemische Industrie (BBCH) decimal code. Plants between 15 and 22 cm were generated, and each phenological growth stage was easily managed in restricted spaces. The time for each phenological growth stage was examined in different amaranth species and this offered a general representation of the phenological growth stages and life cycle of amaranth. This work established the phenological growth stages of amaranth based on the BBCH coding system managed in restricted spaces. These observations allow us to envision amaranth as a model plant in which each phenological growth stage describing its life cycle is managed easily under limited spaces, which could be an advantage for better manipulation and future studies.

Research paper thumbnail of Heavy metal pollution in drinking water - a global risk for human health: A review

African Journal of Environmental Science and Technology, 2013

Water resources in the world have been profoundly influenced over the last years by human activit... more Water resources in the world have been profoundly influenced over the last years by human activities, whereby the world is currently facing critical water supply and drinking water quality problems. In many parts of the world heavy metal (HM) concentrations in drinking water are higher than some international guideline values. Discussing about the HM pollution in drinking water, the incorporation of them into the food chain, and their implications as a global risk for the human health, are the objectives of this review. It is known that there are million people with chronic HM poisoning which has become a worldwide public health issue, while 1.6 million children die each year from diseases for which contaminated drinking water is a leading cause. There is also evidence of HM in drinking water that are responsible for causing adverse effect on human health through food chain contamination. A global effort to offering affordable and healthy drinking water most to be launched throughou...

Research paper thumbnail of Expression and thermal stability of an 11S globulin modified in both, third and fourth variable region

Research paper thumbnail of Comparison of the physicochemical and functional properties of flour and protein isolate from moringa (Moringa oleifera Lam.) leaves

International Journal of Food Properties

Research paper thumbnail of MiR6024 overexpression increases the susceptibility of Nicotiana tabacum to Sclerotinia sclerotiorum

European Journal of Plant Pathology

Research paper thumbnail of Identification of genuine and novel miRNAs in Amaranthus hypochondriacus from high-throughput sequencing data

Genomics

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Shelf Life of Blackberry Fruits (Rubus fruticosus) with Edible Coatings Based on Candelilla Wax and Guar Gum

Horticulturae

Blackberries are very perishable with a limited shelf life due to a high metabolic activity and s... more Blackberries are very perishable with a limited shelf life due to a high metabolic activity and susceptibility to mechanical damage and microbial attack. The effect of edible coatings (EC) based on candelilla wax (CW) and guar gum (GG) on the quality, shelf life, bioactive-compounds content and antioxidant activity of blackberries stored at 25 ± 2 °C for 6 days, was evaluated. All EC contained GG (0.4%) and gallic acid (GA)(0.06%); EC1 contained CW (0.2%), glycerol (GL)(0.2%); EC2 contained CW (0.4%) and GL (0.2%); EC3 contained CW (0.2%) and GL (0.3%) and EC4 contained CW (0.4%) and GL (0.3%). The decay percentage (Decay%), physiological weight loss (%PWL), firmness, pH, total soluble solids (TSS), titratable acidity (TA), total phenolic content (TPC), total anthocyanin content (TAC) and antioxidant activity were analyzed. EC3 showed potential for prolonging the shelf life of blackberry by reducing Decay% (47%) and %PWL (64%) on day 6, while 86% of uncoated blackberries were decaye...

Research paper thumbnail of Effect of supercritical carbon dioxide on physicochemical and techno-functional properties of amaranth flour

Chemical Engineering and Processing - Process Intensification

Research paper thumbnail of Foaming and Structural Studies on the Acidic Subunit of Amaranth 11S Globulin Modified with Antihypertensive Peptides as a Function of pH and Ionic Strength

Molecules

Some studies aimed at revealing the relationship between protein structure and their functional p... more Some studies aimed at revealing the relationship between protein structure and their functional properties. However, the majority of these reports have been carried out using protein isolates. There are limited reports on the possible relationship between the functional properties and the structure of a purified protein. In this work the amaranth 11S globulin acidic subunit (AAC) and five mutations of the same protein that were modified in their variable regions with antihypertensive peptides (VYVYVYVY and RIPP), were analyzed at two ionic strength (2.9 and 17.6 g/L NaCl) and pH (3.0–7.0). Results revealed better solubility for the proteins mutated at the terminal ends (AACM.1 and AACM.4) and lower solubility for the protein inserted with RIPP peptide. Spectroscopy studies revealed an increase of β-sheet structure at high salt concentration for all proteins. It was also observed that salt concentration acted as a modulator, which allowed a better foam features for all modified prote...

Research paper thumbnail of Supplementary material

CERN European Organization for Nuclear Research - Zenodo, Mar 31, 2022

Research paper thumbnail of Growth and characterization of Cd1-xZnxTe (0 \leq x \leq 1) nanolayers grown by isothermal closed space atomic layer deposition on GaSb and GaAs

Revista Mexicana de Física, 2018

In this work are presented the results obtained from the deposition of Cd1-xZnxTe nanolayers usin... more In this work are presented the results obtained from the deposition of Cd1-xZnxTe nanolayers using as precursor the vapours of the elements Zn, Te, and a mixture of Cd and Zn on GaAs and GaSb (001) substrates by Atomic Layer Deposition technique (ALD), which allows the deposition of layers of nanometric dimensions. At each exposure of the growth surface to the of cation or anion precursors vapours, this surface is saturated. Therefore, it is considered that the process is self-regulated. The ZnTe layers were grown in a wide range of temperatures; however, ZnTe nanolayers with a shiny mirror-like surface could be grown at temperatures between 370 and 410oC. Temperatures higher than 400oC were necessary for the CdTe growth. The layers of the Cd1-xZnxTe ternary alloy were deposited at temperature range of 400 and 425oC. The grown nanofilms were characterized by Raman spectroscopy and high-resolution X-ray diffraction. The Raman spectrum shows the peak corresponding to LO-ZnTe at 208 cm...

Research paper thumbnail of Analysis of biodiesel production by transesterification of crude oil from the pulp of class III avocado (Persea Americana Mill var. Hass, Fuerte and Creole)

Interciencia, Nov 1, 2016

Research paper thumbnail of Effect of Ethanol and Methanol on the Total Phenolic Content and Antioxidant Capacity of Chia Seeds (Salvia hispanica L.)

Sains Malaysiana, 2020

The use of methanol and ethanol for the extraction of antioxidant compounds in Chia Seed (Salvia ... more The use of methanol and ethanol for the extraction of antioxidant compounds in Chia Seed (Salvia hispanica L.) is studied, but only the effect of a single concentration of these solvents was evaluated. However, factors such as the nature and the concentration of the solvents determines the content of phenolic compounds, the antioxidant capacity and the possible use of the extract. For this reason, it is necessary to have a detailed study of different extraction conditions to find the optimum. The objective of this work was to evaluate the effect of different concentrations of ethanol and methanol and the hexane treatment on the extraction of phenolic compounds and the antioxidant capacity of the Chia Seeds extracts. The greatest total phenolic content was obtained in the 50% aqueous ethanol and methanol extracts. Nevertheless, all the extracts showed a high DPPH scavenging activity. With exception of 100% ethanol extract, no significant differences were found between the extracts treated with and without hexane. No significant differences were found in the extraction yields of the 80% aqueous ethanol and methanol extracts, but the aqueous methanol extract presented lower IC 50. The FTIR and UV-VIS spectra of the aqueous ethanol and methanol Chia extracts suggest differences in the composition of the extracts. It is possible to extract the phenolic compounds from the Chia Seeds with different concentration of ethanol and methanol, which will allow to select the appropriate extraction conditions according to the application of the extract.

Research paper thumbnail of Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis

European Food Research and Technology, 2017

Enzymatic hydrolysis of migratory locust (Locusta migratoria L.) protein flour (MLPF) was investi... more Enzymatic hydrolysis of migratory locust (Locusta migratoria L.) protein flour (MLPF) was investigated as a method to improve the techno-functional properties. Experiments were conducted under variation of the applied proteases (Alcalase, Neutrase, Flavourzyme, Papain) or combinations thereof, enzyme-substrate ratio (0.05-1.0% w/w), heat pre-treatment (60-80 °C; 15-60 min), and hydrolysis time (0-24 h). Protein degradation was monitored in terms of degree of hydrolysis (DH) and SDS-PAGE. Solubility, emulsifying, foaming and water/oil binding properties of the hydrolysates were determined. In comparison to the control (DH = 5%), hydrolysis resulted in considerably higher DH values up to 42%. SDS-PAGE profiles revealed a steady decrease of bands between 25 and 75 kDa and an increase of low molecular weight bands (10-15 kDa). However, different heat pre-treatments resulted in impaired hydrolytic cleavage as evidenced by lower DH values. Protein solubility of MLPF hydrolysates was improved over a broad pH range from initially 10-22% up to 55% at alkaline conditions. Furthermore, hydrolysis resulted in enhanced emulsifying activity (54%) at pH 7, improved foamability (326%) at pH 3 and advanced oil binding capacity. The results of this study have clearly demonstrated the potential of targeted enzymatic degradation to improve the techno-functionality of migratory locust protein in order to produce tailored insect-based ingredients for the use in food applications.

Research paper thumbnail of Certificate of Attendence

Research paper thumbnail of EVALUACIÓN DEL POTENCIAL PROBIÓTICOY CREClMIENTO EN DIFERENTES MEDIOS DE CULTIVO DE CEPAS DE LACTOBACILLUS SP

Research paper thumbnail of Extraction methods and nutritional characterization of protein concentrates obtained from bean, chickpea, and corn discard grains

Current Research in Food Science

Research paper thumbnail of Simple and efficient protocol for amaranth betalains extraction and stability analysis by ATR-FTIR spectroscopy

Journal of Cereal Science

Research paper thumbnail of Nutritional Characterization of Protein Concentrates Obtained from Bean, Chickpea, And Corn Discard Grains

Social Science Research Network, 2022

Research paper thumbnail of Desiccation-induced viable but nonculturable state in Pseudomonas putida KT2440, a survival strategy

PLOS ONE, 2019

S10 Fig. Amplifica6on of the16S rRNA gene from P. pu&da KT2440 by using the RT-PCR method. 1) Mar... more S10 Fig. Amplifica6on of the16S rRNA gene from P. pu&da KT2440 by using the RT-PCR method. 1) Marker 1 kb DNA Leader Jena Bioscience, 2) cells before desicca;on, 3) twenty-min rehydrated cells of 18 DABD. 4) Twenty four-hours rehydrated cells of 18 DABD, 5) twenty-min rehydrated cells of 40 DABD, 6) nega;ve control; reac;on without retrotranscriptase, and 7) nega;ve control; reac;on without template.

Research paper thumbnail of The phenological growth stages of different amaranth species grown in restricted spaces based in BBCH code

South African Journal of Botany, 2019

Amaranth is a pseudocereal with potential health benefits. Amaranth has recently gained importanc... more Amaranth is a pseudocereal with potential health benefits. Amaranth has recently gained importance due to its high capacity to grow in adverse conditions. Details about the growth and development of amaranth is fundamental to its cultivation, but reports on the phenological growth stages, development, and the life cycle of amaranth are limited. Under normal conditions, amaranth plants are as high as 2.2 m, making their handling difficult. Thus, this study determined the phenological growth stages and life cycle of amaranth in restricted spaces. Amaranthus cruentus, Amaranthus hybridus, and Amaranthus hypochondriacus plants were cultivated in restricted spaces. The physiological and qualitative features as number of leaves, length of plants and leaves, panicle color, were used to determine the different phenological growth stages and life cycle of amaranth plants. The phenological growth stages were described vía Biologische Bundesanstalt Bundessortenamt and CHemische Industrie (BBCH) decimal code. Plants between 15 and 22 cm were generated, and each phenological growth stage was easily managed in restricted spaces. The time for each phenological growth stage was examined in different amaranth species and this offered a general representation of the phenological growth stages and life cycle of amaranth. This work established the phenological growth stages of amaranth based on the BBCH coding system managed in restricted spaces. These observations allow us to envision amaranth as a model plant in which each phenological growth stage describing its life cycle is managed easily under limited spaces, which could be an advantage for better manipulation and future studies.

Research paper thumbnail of Heavy metal pollution in drinking water - a global risk for human health: A review

African Journal of Environmental Science and Technology, 2013

Water resources in the world have been profoundly influenced over the last years by human activit... more Water resources in the world have been profoundly influenced over the last years by human activities, whereby the world is currently facing critical water supply and drinking water quality problems. In many parts of the world heavy metal (HM) concentrations in drinking water are higher than some international guideline values. Discussing about the HM pollution in drinking water, the incorporation of them into the food chain, and their implications as a global risk for the human health, are the objectives of this review. It is known that there are million people with chronic HM poisoning which has become a worldwide public health issue, while 1.6 million children die each year from diseases for which contaminated drinking water is a leading cause. There is also evidence of HM in drinking water that are responsible for causing adverse effect on human health through food chain contamination. A global effort to offering affordable and healthy drinking water most to be launched throughou...

Research paper thumbnail of Expression and thermal stability of an 11S globulin modified in both, third and fourth variable region

Research paper thumbnail of Comparison of the physicochemical and functional properties of flour and protein isolate from moringa (Moringa oleifera Lam.) leaves

International Journal of Food Properties

Research paper thumbnail of MiR6024 overexpression increases the susceptibility of Nicotiana tabacum to Sclerotinia sclerotiorum

European Journal of Plant Pathology

Research paper thumbnail of Identification of genuine and novel miRNAs in Amaranthus hypochondriacus from high-throughput sequencing data

Genomics

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Shelf Life of Blackberry Fruits (Rubus fruticosus) with Edible Coatings Based on Candelilla Wax and Guar Gum

Horticulturae

Blackberries are very perishable with a limited shelf life due to a high metabolic activity and s... more Blackberries are very perishable with a limited shelf life due to a high metabolic activity and susceptibility to mechanical damage and microbial attack. The effect of edible coatings (EC) based on candelilla wax (CW) and guar gum (GG) on the quality, shelf life, bioactive-compounds content and antioxidant activity of blackberries stored at 25 ± 2 °C for 6 days, was evaluated. All EC contained GG (0.4%) and gallic acid (GA)(0.06%); EC1 contained CW (0.2%), glycerol (GL)(0.2%); EC2 contained CW (0.4%) and GL (0.2%); EC3 contained CW (0.2%) and GL (0.3%) and EC4 contained CW (0.4%) and GL (0.3%). The decay percentage (Decay%), physiological weight loss (%PWL), firmness, pH, total soluble solids (TSS), titratable acidity (TA), total phenolic content (TPC), total anthocyanin content (TAC) and antioxidant activity were analyzed. EC3 showed potential for prolonging the shelf life of blackberry by reducing Decay% (47%) and %PWL (64%) on day 6, while 86% of uncoated blackberries were decaye...

Research paper thumbnail of Effect of supercritical carbon dioxide on physicochemical and techno-functional properties of amaranth flour

Chemical Engineering and Processing - Process Intensification

Research paper thumbnail of Foaming and Structural Studies on the Acidic Subunit of Amaranth 11S Globulin Modified with Antihypertensive Peptides as a Function of pH and Ionic Strength

Molecules

Some studies aimed at revealing the relationship between protein structure and their functional p... more Some studies aimed at revealing the relationship between protein structure and their functional properties. However, the majority of these reports have been carried out using protein isolates. There are limited reports on the possible relationship between the functional properties and the structure of a purified protein. In this work the amaranth 11S globulin acidic subunit (AAC) and five mutations of the same protein that were modified in their variable regions with antihypertensive peptides (VYVYVYVY and RIPP), were analyzed at two ionic strength (2.9 and 17.6 g/L NaCl) and pH (3.0–7.0). Results revealed better solubility for the proteins mutated at the terminal ends (AACM.1 and AACM.4) and lower solubility for the protein inserted with RIPP peptide. Spectroscopy studies revealed an increase of β-sheet structure at high salt concentration for all proteins. It was also observed that salt concentration acted as a modulator, which allowed a better foam features for all modified prote...

Research paper thumbnail of Supplementary material

CERN European Organization for Nuclear Research - Zenodo, Mar 31, 2022

Research paper thumbnail of Growth and characterization of Cd1-xZnxTe (0 \leq x \leq 1) nanolayers grown by isothermal closed space atomic layer deposition on GaSb and GaAs

Revista Mexicana de Física, 2018

In this work are presented the results obtained from the deposition of Cd1-xZnxTe nanolayers usin... more In this work are presented the results obtained from the deposition of Cd1-xZnxTe nanolayers using as precursor the vapours of the elements Zn, Te, and a mixture of Cd and Zn on GaAs and GaSb (001) substrates by Atomic Layer Deposition technique (ALD), which allows the deposition of layers of nanometric dimensions. At each exposure of the growth surface to the of cation or anion precursors vapours, this surface is saturated. Therefore, it is considered that the process is self-regulated. The ZnTe layers were grown in a wide range of temperatures; however, ZnTe nanolayers with a shiny mirror-like surface could be grown at temperatures between 370 and 410oC. Temperatures higher than 400oC were necessary for the CdTe growth. The layers of the Cd1-xZnxTe ternary alloy were deposited at temperature range of 400 and 425oC. The grown nanofilms were characterized by Raman spectroscopy and high-resolution X-ray diffraction. The Raman spectrum shows the peak corresponding to LO-ZnTe at 208 cm...

Research paper thumbnail of Analysis of biodiesel production by transesterification of crude oil from the pulp of class III avocado (Persea Americana Mill var. Hass, Fuerte and Creole)

Interciencia, Nov 1, 2016

Research paper thumbnail of Effect of Ethanol and Methanol on the Total Phenolic Content and Antioxidant Capacity of Chia Seeds (Salvia hispanica L.)

Sains Malaysiana, 2020

The use of methanol and ethanol for the extraction of antioxidant compounds in Chia Seed (Salvia ... more The use of methanol and ethanol for the extraction of antioxidant compounds in Chia Seed (Salvia hispanica L.) is studied, but only the effect of a single concentration of these solvents was evaluated. However, factors such as the nature and the concentration of the solvents determines the content of phenolic compounds, the antioxidant capacity and the possible use of the extract. For this reason, it is necessary to have a detailed study of different extraction conditions to find the optimum. The objective of this work was to evaluate the effect of different concentrations of ethanol and methanol and the hexane treatment on the extraction of phenolic compounds and the antioxidant capacity of the Chia Seeds extracts. The greatest total phenolic content was obtained in the 50% aqueous ethanol and methanol extracts. Nevertheless, all the extracts showed a high DPPH scavenging activity. With exception of 100% ethanol extract, no significant differences were found between the extracts treated with and without hexane. No significant differences were found in the extraction yields of the 80% aqueous ethanol and methanol extracts, but the aqueous methanol extract presented lower IC 50. The FTIR and UV-VIS spectra of the aqueous ethanol and methanol Chia extracts suggest differences in the composition of the extracts. It is possible to extract the phenolic compounds from the Chia Seeds with different concentration of ethanol and methanol, which will allow to select the appropriate extraction conditions according to the application of the extract.

Research paper thumbnail of Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis

European Food Research and Technology, 2017

Enzymatic hydrolysis of migratory locust (Locusta migratoria L.) protein flour (MLPF) was investi... more Enzymatic hydrolysis of migratory locust (Locusta migratoria L.) protein flour (MLPF) was investigated as a method to improve the techno-functional properties. Experiments were conducted under variation of the applied proteases (Alcalase, Neutrase, Flavourzyme, Papain) or combinations thereof, enzyme-substrate ratio (0.05-1.0% w/w), heat pre-treatment (60-80 °C; 15-60 min), and hydrolysis time (0-24 h). Protein degradation was monitored in terms of degree of hydrolysis (DH) and SDS-PAGE. Solubility, emulsifying, foaming and water/oil binding properties of the hydrolysates were determined. In comparison to the control (DH = 5%), hydrolysis resulted in considerably higher DH values up to 42%. SDS-PAGE profiles revealed a steady decrease of bands between 25 and 75 kDa and an increase of low molecular weight bands (10-15 kDa). However, different heat pre-treatments resulted in impaired hydrolytic cleavage as evidenced by lower DH values. Protein solubility of MLPF hydrolysates was improved over a broad pH range from initially 10-22% up to 55% at alkaline conditions. Furthermore, hydrolysis resulted in enhanced emulsifying activity (54%) at pH 7, improved foamability (326%) at pH 3 and advanced oil binding capacity. The results of this study have clearly demonstrated the potential of targeted enzymatic degradation to improve the techno-functionality of migratory locust protein in order to produce tailored insect-based ingredients for the use in food applications.

Research paper thumbnail of Certificate of Attendence

Research paper thumbnail of EVALUACIÓN DEL POTENCIAL PROBIÓTICOY CREClMIENTO EN DIFERENTES MEDIOS DE CULTIVO DE CEPAS DE LACTOBACILLUS SP