Simone Mossini - Academia.edu (original) (raw)
Papers by Simone Mossini
Química Nova, 2021
Deborah Thais Palma Scanferlaa, Jessica Yuri Sakuradaa, Luís Otávio de Oliveiraa, Renata Sano Lin... more Deborah Thais Palma Scanferlaa, Jessica Yuri Sakuradaa, Luís Otávio de Oliveiraa, Renata Sano Linia, Raul Gomes Agueraa, Mariana Aparecida Oliveira Madiaa, Paula Pessoa Moreiraa, Jessica Cristina Zoratto Romolib, Érika Bandob, Miguel Machinski Juniorb, Camila Marchionic and Simone Aparecida Galerani Mossinib,*, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, 87020-900 Maringá – PR, Brasil Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, 87020-900 Maringá – PR, Brasil Departamento de Patologia, Universidade Federal de Santa Catarina, 88040-370 Florianópolis – SC, Brasil
The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrin... more The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrine alterations, caused by chemical residues in food, has led to the search for sustainable agricultural management alternatives, such as the use of essential oils for the development of natural and eco-friendly fungicides. The aim of this study was to evaluate the antifungal and antiaflatoxigenic activity of <i>Rosmarinus officinalis</i> L. essential oil (REO) against <i>Aspergillus flavus</i> Link. REO was obtained by hydrodistillation and its major components were identified as 1,8-cineole (eucalyptol, 52.2%), camphor (15.2%) and α-pinene (12.4%) by GC/MS and NMR. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were both 500 µg/mL. REO reduced the mycelial growth of <i>A. flavus</i> at a concentration of 250 µg/mL (15.3%). The results obtained from scanning electron microscopy (SEM) demonstrated a reduction in the...
Com a evolucao na producao agricola, o uso de praguicidas em larga escala tornou-se algo necessar... more Com a evolucao na producao agricola, o uso de praguicidas em larga escala tornou-se algo necessario para manter a demanda produtiva. A utilizacao destes produtos de forma descontrolada propicia aparecimento de casos de intoxicacao. Dentre os praguicidas utilizados atualmente, os inseticidas organofosforados e carbamatos, causam intoxicacoes graves que podem evoluir para o obito. O mecanismo de acao e a inibicao da enzima acetilcolinesterase, responsavel pela hidrolise da acetilcolina, com a enzima inibida ocorre o acumulo do neurotransmissor, exacerbando seus efeitos fisiologicos. O objetivo deste trabalho foi analisar a exposicao ocupacional de viticultores de Marialva-PR aos praguicidas inibidores da colinesterase. Trata-se de um estudo descritivo transversal realizado com a populacao de viticultores de Marialva-PR, Brasil, exposta aos inibidores da colinesterase, durante o ano de 2017, entre os meses de junho a julho (primeira etapa) e setembro a novembro (segunda etapa). Neste e...
Objective: To analyze the data on epidemiological records of autointoxication reports registered ... more Objective: To analyze the data on epidemiological records of autointoxication reports registered at a toxicological assistance center. Methods: Exploratory, descriptive, and retrospective study with the analysis of secondary data, conducted from the audit on Toxicological Occurrence Notification and Attendance forms, referring to the period from 2014 to 2018, filed in a toxicological information and assistance center, located in a municipality in southern Brazil. The data obtained were compiled in spreadsheets and analyzed using simple descriptive statistics. Results: 2,942 epidemiological records on women's autointoxication were audited, 72.0% of the total cases in the period studied. The predominant age group was 18 to 29 years old, with 1697 (57.7%) cases. The main toxic agent was medication, and the only agent of intoxication in 2,358 cases (80.1%). Psychotropic drugs accounted for 55.2% (1,593) of the cases, mainly antiepileptic and psychoanalytic drugs. Clonazepam was pres...
Tendo como objetivo conhecer a prevalencia e as caracteristicas do uso de tabaco e seus derivados... more Tendo como objetivo conhecer a prevalencia e as caracteristicas do uso de tabaco e seus derivados entre universitarios da area de saude, buscou-se caracterizar o consumo de tabaco do curso de biomedicina de uma universidade publica. Trata-se de estudo transversal, quantitativo, com dados obtidos atraves de questionario anonimo, auto aplicado, respondido em sala de aula de turno integral, no ano de 2018. Participaram voluntariamente da pesquisa 104 universitarios. Com os dados obtidos, evidencia-se deficiencias na informacao em relacao as condutas que ocasionam riscos a saude. Como futuros profissionais, esses formandos serao responsaveis por orientar a populacao acerca do uso de substâncias toxicas, tais como o tabaco, evidenciando a necessidade de modificacoes na formacao academica para melhor exercicio da profissao.
EnglishEssential oils are possible alternatives to the use of synthetic pesticides for control of... more EnglishEssential oils are possible alternatives to the use of synthetic pesticides for control of fungal contamination. Ginger (Zingiber officinale) essential oil (GEO) is known for having antifungal and antiaflatoxigenic properties, but its use as a fumigant in situ has not been studied yet. The aim of this study was to evaluate GEO’s effects upon Aspergillus flavus as a fumigant agent in stored maize grains. The main compounds reported in GEO were α-zingiberene (23.85%) and geranial (14.16%), characterized by gas chromatography-mass spectrometry and nuclear magnetic resonance. The GEO was used as a fumigant in irradiated maize grains in concentrations ranging from 5 to 50 µg/g and the resulting effects were compared to a synthetic antifungal agent (carbendazim and thiram), an antifungal traditionally used for seed treatment. The antifungal efficacy of GEO against A. flavus has been proven in a dose-dependent manner through in situ (maize grains) test. The GEO inhibited aflatoxin p...
Submitted 15 October 2020, Resubmitted 14 December 2020, 2nd time Resubmitted 11 February 21, Acc... more Submitted 15 October 2020, Resubmitted 14 December 2020, 2nd time Resubmitted 11 February 21, Accepted 11 February 21, Available online March 2021. Cite: de Oliveira, K. O.; Scanferla, D. T. P.; da Silva, J. Q. P.; Madia, M. A. O.; Bando, E.; Machinski Junior, M.; Marchioni, C.; Mossini, S. A. G. Quantitative analysis of Δ9-THC-COOH in Human Urine by the Liquid-Liquid Extraction technique and Gas Chromatography-Mass Spectrometry: Adaptation, Optimization and Validation. Braz. J. Anal. Chem., 2021, 8 (00), pp 00–00. doi: http://dx.doi.org/10.30744/brjac.2179-3425.TN-59-2020 Brazilian Journal of Analytical Chemistry Pre-publication – Accepted after peer review doi: 10.30744/brjac.2179-3425.TN-59-2020
Environmental Science and Pollution Research
This is a cross-sectional study with data and biological material collection from vineyard farmer... more This is a cross-sectional study with data and biological material collection from vineyard farmers in southern Brazil. An interview was carried out through a questionnaire developed according to the reference guide of the state government. Plasma and urine samples were screened for Aluminum, Chromium, Manganese, Copper, Nickel, Cobalt, Zinc, Arsenic, Selenium, Cadmium, Antimony, Barium, Mercury, Lead and Uranium, with a technique for fast determination of these elemental contents in biological material utilizing dynamic reaction cell inductively coupled mass spectrometry. Principal component analysis was used to identify associations between these elemental contents in biological samples and the information obtained from the interviews. The farmers showed some trace elements in plasma and urine at a higher concentration than unexposed populations from other studies. This study highlights recent findings of trace elements in biological material and their association with characteristics of pesticide use. In addition, it also contributes to the gap in the literature regarding trace elements content in plasma and urine of workers exposed to pesticides.
Research, Society and Development
Pesquisas abordando o tema da violência autoinfligida são relevantes, mas têm sido pouco abordado... more Pesquisas abordando o tema da violência autoinfligida são relevantes, mas têm sido pouco abordados no Brasil. Este estudo tem como objetivo analisar dados sobre notificações de mortes por lesões autoprovocadas no estado do Paraná. Trata-se de um estudo ecológico, descritivo, de abordagem quantitativa a partir de dados secundários obtidos dos sistemas de informação de saúde nacionais de casos notificados de óbitos por lesão autoprovocada intencionalmente no estado do Paraná, no período de 2009 a 2018, referente ao Código Internacional da Doença (CID) 10 da categoria X60 a X84. Foram identificados 6609 óbitos por lesões autoprovocadas com crescimento significativo (p<0,0001) no período analisado, com predominância do sexo masculino (79,69%), solteiro (49,27%), com maior ocorrência em domicílio (64,26%) e faixa etária entre 20 a 59 anos. A principal causa de óbito foi a lesão autoprovocada por enforcamento, estrangulamento e sufocação. Autointoxicação por medicamentos e drogas de ab...
Saúde e Pesquisa
O objetivo desta pesquisa foi conhecer o consumo de álcool entre estudantes de uma universidade n... more O objetivo desta pesquisa foi conhecer o consumo de álcool entre estudantes de uma universidade no sul do Brasil. Realizou-se estudo transversal, quantitativo, com acadêmicos de Biomedicina (n=134) por meio do instrumento Alcohol Use Disorders Identification Test, versão traduzida. Utilizou-se teste estatístico para verificar associação entre variáveis categóricas. Houve predomínio de mulheres, de 18 a 21 anos. Verificou-se início precoce de consumo de álcool, anterior à chegada à universidade, vinculado a festas e companhia de amigos. Após ingressar no curso, 41,04% disseram ter aumentado a ingestão (p=0,0001). Quanto ao risco, 72,38% foram classificados como consumidores de baixo risco, 19,39% como de risco, 5,98% de alto risco e 2,25% dependentes. Ser acadêmico da área da saúde e conhecer os prejuízos associados ao consumo do álcool não favorece a adoção de um estilo de vida saudável. Evidencia-se que o uso de álcool não é exclusivamente influenciado pelo conhecimento dos riscos.
Food and Chemical Toxicology
This study aimed to evaluate the risk concerning child population's health because of the occ... more This study aimed to evaluate the risk concerning child population's health because of the occurrence of AFM1 in UHT milk, powdered milk (PM) and infant formulae (IF). Determination of AFM1 was performed in 60 samples and evaluation of the mycotoxin exposure was carried out through the determination of the estimated daily intake (EDI), whereas risk characterization was evaluated with the calculation of the risk of Hepatocellular Carcinoma (HCC) and the Margin of Exposure (MOE). AFM1 ranged from 150 to 1,020 ng/kg, and all the positive samples exceeded the limits stablished by European Community. The EDI for AFM1 ranged according to the age group of the population studied (0-5 years old) from 0.828-2.523, 0-2.113 and 0.029-0.833 ng/kg b.w./day in UHT, PM and IF, respectively. The number of HCC cases associated with AFM1 exposure (0.0015 a 0.0045) was higher than the limit of 0.001 case/100,000. MOE values for AFM1 were 728 to 239, considerably below the security margin of 10,000. These results point to a potential risk to the health of Brazilian child population exposed to AFM1 in dairy products.
Brazilian Journal of Pharmaceutical Sciences
Research, Society and Development
A exposição crônica aos agrotóxicos pode levar ao desenvolvimento de doenças, e a escassez de inf... more A exposição crônica aos agrotóxicos pode levar ao desenvolvimento de doenças, e a escassez de informações sobre manifestações clínicas na exposição a longo prazo é uma preocupação atual. Este trabalho teve como objetivo analisar o uso crônico de agrotóxicos da classe dos fungicidas por viticultores de um município do Paraná, região sul do Brasil. Foram entrevistados 192 agricultores familiares, que residiam em 66 propriedades, com instrumentos desenvolvidos segundo o protocolo de avaliação das intoxicações crônicas por agrotóxicos da Secretaria de Estado da Saúde do Paraná. Entre os participantes, 45,8% trabalhava há mais de 20 anos na viticultura, utilizando continuamente os fungicidas e apenas 6,8% relatou uso de todos os equipamentos de proteção recomendados para a aplicação dos agrotóxicos. Foram relatados sintomas como irritação cutânea pruriginosa (20,5%) e tremores (12,1%) que podem estar associados a exposição crônica aos fungicidas ditiocarbamatos. A população agricultora f...
Food Additives & Contaminants: Part A
ABSTRACT There is an increasing demand for fungi control in grains, especially toxigenic. Also, t... more ABSTRACT There is an increasing demand for fungi control in grains, especially toxigenic. Also, there is growing concern on the use of synthetic fungicides; thus alternatives are needed. The aim of this study was to evaluate the antifungal and antimycotoxigenic action of essential oils (EOs) from Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii against Fusarium verticillioides, a spoilage and toxigenic fungus. Essential oils were first chemically characterised by gas chromatography coupled to mass spectrometry, and their antioxidant potential was measured by the DPPH, ABTS and FRAP methods. Minimum inhibitory concentration (MIC) and disc diffusion were used to assess antifungal activity. Scanning electron microscopy was used to evaluate morphological changes in the fungus. Antimycotoxigenic activity of the EOs against the production of fumonisin B1 and B2 by F. verticillioides was evaluated using ultra-high-performance liquid chromatography system. Z. officinale, C. zeylanicum and C. martinii EOs were predominantly composed by zingiberene and geranial; eugenol; and geraniol, respectively. All the EOs had high antioxidant power, especially that from C. zeylanicum. The MICs were 250, 500 and 2,000 µg mL−1 for C. zeylanicum, C. martinii and Z. officinale EOs, respectively. Mycelial reduction of F. verticillioides was observed when EOs were used, and the lowest activity was detected in the Z. officinale EO. Overall, the tested EOs promoted structural damage to the fungal cell wall, decreased conidia size and mycelial reduction. Antimycotoxigenic evaluation of the EOs evidenced a significant reduction (p < .05) in the production of fumonisins B1 and B2 with all the EOs evaluated in the study. These results suggest that especially C. zeylanicum and C. martinii EOs are highly useful for controlling F. verticillioides and fumonisins production. GRAPHICAL ABSTRACT
Food Additives & Contaminants: Part A
ABSTRACT The increased risk to health by diverse pathologies, such as cancer, liver diseases, and... more ABSTRACT The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrine alterations, caused by chemical residues in food, has led to the search for sustainable agricultural management alternatives, such as the use of essential oils for the development of natural and eco-friendly fungicides. The aim of this study was to evaluate the antifungal and antiaflatoxigenic activity of Rosmarinus officinalis L. essential oil (REO) against Aspergillus flavus Link. REO was obtained by hydrodistillation and its major components were identified as 1,8-cineole (eucalyptol, 52.2%), camphor (15.2%) and α-pinene (12.4%) by GC/MS and NMR. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were both 500 µg/mL. REO reduced the mycelial growth of A. flavus at a concentration of 250 µg/mL (15.3%). The results obtained from scanning electron microscopy (SEM) demonstrated a reduction in the size of conidiophores and in the thickness of hyphae in A. flavus caused by treatment with REO (250 µg/mL). The production of ergosterol and the biomass of mycelium were both reduced as the REO treatment concentration increased. The production of aflatoxins B1 and B2 was inhibited after treatment with 250 µg/mL REO, a concentration below the MIC/MFC, indicating that the antiaflatoxigenic effect of REO is independent of its antifungal effect and is likely due to its direct action upon toxin biosynthesis. The data demonstrated that REO may be used as an alternative to synthetic fungicides. Graphical abstract
Journal of Food Safety
The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and c... more The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and commercialized in State of Parana, Brazil. The extraction method carried out with a Romer Mycosep 226 column was found to be efficient due to addition of both diatomaceous earth and centrifuging during the cleaning step. From the total of 84 analyzed samples, 78.6% (n = 66) were found to be contaminated ZEA at levels between 15.4 and 72.1 μg/kg. This study was the first report on occurrence of positive samples for ZEA in corn meal commercialized in Parana since 2005. The estimated daily intake (EDI) of ZEA for the Brazilian population and for the State of Parana was 3.1 and 2.2 ng/kg bw/day, respectively. The EDI was below the tolerable daily intake of 250 ng/kg bw/day. Despite the low health risk of the population, it was observed that the occurrence of ZEA in corn meal was frequent, which demonstrates the importance of monitoring studies to ensure consumer's health. PRACTICAL APPLICATIONS: Occurrence of zearalenone in corn meal was high (78.6%) in Brazil. First report on corn meal samples positive for zearalenone in Parana, Brazil. No sample exceeded the tolerated limit by both Brazilian and European legislation. EDI due to corn meal consumption was inferior to TDI.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 2018
Members of the Fusarium genus are capable of contaminating agricultural commodities, compromising... more Members of the Fusarium genus are capable of contaminating agricultural commodities, compromising the quality of maize and other grains, which leads to severe quality and yield losses. Contamination with mycotoxins is also a concern. Essential oils are possible alternatives to the use of synthetic pesticides for control of fungal contamination, as many have antifungal and anti-mycotoxigenic properties and are innocuous to human health. They also do not cause any sort of microbial resistance and do not promote environmental pollution. The aim of this study was to evaluate the antifungal and anti-mycotoxigenic effects of Zingiber officinale Roscoe essential oil (GEO) upon Fusarium graminearum Schwabe in vitro. The essential oil was extracted by hydrodistillation and analysed by GC/MS. Antifungal and anti-mycotoxigenic activities were assessed by HPLC/UV by quantifying ergosterol and deoxynivalenol (DON), respectively. Results indicated that GEO inhibited ergosterol production at a con...
Acta Farmaceutica Bonaerense, 2005
... heterocíclicas 100 . Folhas de Nim podem atuar como mediador na ativação da resposta imune. B... more ... heterocíclicas 100 . Folhas de Nim podem atuar como mediador na ativação da resposta imune. Baral &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Chatto-padhyay 75, estudando em ratos o efeito profilá-tico e terapêutico ... co ao homem. Kasturi et al. 117 verificaram ...
Química Nova, 2021
Deborah Thais Palma Scanferlaa, Jessica Yuri Sakuradaa, Luís Otávio de Oliveiraa, Renata Sano Lin... more Deborah Thais Palma Scanferlaa, Jessica Yuri Sakuradaa, Luís Otávio de Oliveiraa, Renata Sano Linia, Raul Gomes Agueraa, Mariana Aparecida Oliveira Madiaa, Paula Pessoa Moreiraa, Jessica Cristina Zoratto Romolib, Érika Bandob, Miguel Machinski Juniorb, Camila Marchionic and Simone Aparecida Galerani Mossinib,*, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, 87020-900 Maringá – PR, Brasil Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, 87020-900 Maringá – PR, Brasil Departamento de Patologia, Universidade Federal de Santa Catarina, 88040-370 Florianópolis – SC, Brasil
The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrin... more The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrine alterations, caused by chemical residues in food, has led to the search for sustainable agricultural management alternatives, such as the use of essential oils for the development of natural and eco-friendly fungicides. The aim of this study was to evaluate the antifungal and antiaflatoxigenic activity of <i>Rosmarinus officinalis</i> L. essential oil (REO) against <i>Aspergillus flavus</i> Link. REO was obtained by hydrodistillation and its major components were identified as 1,8-cineole (eucalyptol, 52.2%), camphor (15.2%) and α-pinene (12.4%) by GC/MS and NMR. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were both 500 µg/mL. REO reduced the mycelial growth of <i>A. flavus</i> at a concentration of 250 µg/mL (15.3%). The results obtained from scanning electron microscopy (SEM) demonstrated a reduction in the...
Com a evolucao na producao agricola, o uso de praguicidas em larga escala tornou-se algo necessar... more Com a evolucao na producao agricola, o uso de praguicidas em larga escala tornou-se algo necessario para manter a demanda produtiva. A utilizacao destes produtos de forma descontrolada propicia aparecimento de casos de intoxicacao. Dentre os praguicidas utilizados atualmente, os inseticidas organofosforados e carbamatos, causam intoxicacoes graves que podem evoluir para o obito. O mecanismo de acao e a inibicao da enzima acetilcolinesterase, responsavel pela hidrolise da acetilcolina, com a enzima inibida ocorre o acumulo do neurotransmissor, exacerbando seus efeitos fisiologicos. O objetivo deste trabalho foi analisar a exposicao ocupacional de viticultores de Marialva-PR aos praguicidas inibidores da colinesterase. Trata-se de um estudo descritivo transversal realizado com a populacao de viticultores de Marialva-PR, Brasil, exposta aos inibidores da colinesterase, durante o ano de 2017, entre os meses de junho a julho (primeira etapa) e setembro a novembro (segunda etapa). Neste e...
Objective: To analyze the data on epidemiological records of autointoxication reports registered ... more Objective: To analyze the data on epidemiological records of autointoxication reports registered at a toxicological assistance center. Methods: Exploratory, descriptive, and retrospective study with the analysis of secondary data, conducted from the audit on Toxicological Occurrence Notification and Attendance forms, referring to the period from 2014 to 2018, filed in a toxicological information and assistance center, located in a municipality in southern Brazil. The data obtained were compiled in spreadsheets and analyzed using simple descriptive statistics. Results: 2,942 epidemiological records on women's autointoxication were audited, 72.0% of the total cases in the period studied. The predominant age group was 18 to 29 years old, with 1697 (57.7%) cases. The main toxic agent was medication, and the only agent of intoxication in 2,358 cases (80.1%). Psychotropic drugs accounted for 55.2% (1,593) of the cases, mainly antiepileptic and psychoanalytic drugs. Clonazepam was pres...
Tendo como objetivo conhecer a prevalencia e as caracteristicas do uso de tabaco e seus derivados... more Tendo como objetivo conhecer a prevalencia e as caracteristicas do uso de tabaco e seus derivados entre universitarios da area de saude, buscou-se caracterizar o consumo de tabaco do curso de biomedicina de uma universidade publica. Trata-se de estudo transversal, quantitativo, com dados obtidos atraves de questionario anonimo, auto aplicado, respondido em sala de aula de turno integral, no ano de 2018. Participaram voluntariamente da pesquisa 104 universitarios. Com os dados obtidos, evidencia-se deficiencias na informacao em relacao as condutas que ocasionam riscos a saude. Como futuros profissionais, esses formandos serao responsaveis por orientar a populacao acerca do uso de substâncias toxicas, tais como o tabaco, evidenciando a necessidade de modificacoes na formacao academica para melhor exercicio da profissao.
EnglishEssential oils are possible alternatives to the use of synthetic pesticides for control of... more EnglishEssential oils are possible alternatives to the use of synthetic pesticides for control of fungal contamination. Ginger (Zingiber officinale) essential oil (GEO) is known for having antifungal and antiaflatoxigenic properties, but its use as a fumigant in situ has not been studied yet. The aim of this study was to evaluate GEO’s effects upon Aspergillus flavus as a fumigant agent in stored maize grains. The main compounds reported in GEO were α-zingiberene (23.85%) and geranial (14.16%), characterized by gas chromatography-mass spectrometry and nuclear magnetic resonance. The GEO was used as a fumigant in irradiated maize grains in concentrations ranging from 5 to 50 µg/g and the resulting effects were compared to a synthetic antifungal agent (carbendazim and thiram), an antifungal traditionally used for seed treatment. The antifungal efficacy of GEO against A. flavus has been proven in a dose-dependent manner through in situ (maize grains) test. The GEO inhibited aflatoxin p...
Submitted 15 October 2020, Resubmitted 14 December 2020, 2nd time Resubmitted 11 February 21, Acc... more Submitted 15 October 2020, Resubmitted 14 December 2020, 2nd time Resubmitted 11 February 21, Accepted 11 February 21, Available online March 2021. Cite: de Oliveira, K. O.; Scanferla, D. T. P.; da Silva, J. Q. P.; Madia, M. A. O.; Bando, E.; Machinski Junior, M.; Marchioni, C.; Mossini, S. A. G. Quantitative analysis of Δ9-THC-COOH in Human Urine by the Liquid-Liquid Extraction technique and Gas Chromatography-Mass Spectrometry: Adaptation, Optimization and Validation. Braz. J. Anal. Chem., 2021, 8 (00), pp 00–00. doi: http://dx.doi.org/10.30744/brjac.2179-3425.TN-59-2020 Brazilian Journal of Analytical Chemistry Pre-publication – Accepted after peer review doi: 10.30744/brjac.2179-3425.TN-59-2020
Environmental Science and Pollution Research
This is a cross-sectional study with data and biological material collection from vineyard farmer... more This is a cross-sectional study with data and biological material collection from vineyard farmers in southern Brazil. An interview was carried out through a questionnaire developed according to the reference guide of the state government. Plasma and urine samples were screened for Aluminum, Chromium, Manganese, Copper, Nickel, Cobalt, Zinc, Arsenic, Selenium, Cadmium, Antimony, Barium, Mercury, Lead and Uranium, with a technique for fast determination of these elemental contents in biological material utilizing dynamic reaction cell inductively coupled mass spectrometry. Principal component analysis was used to identify associations between these elemental contents in biological samples and the information obtained from the interviews. The farmers showed some trace elements in plasma and urine at a higher concentration than unexposed populations from other studies. This study highlights recent findings of trace elements in biological material and their association with characteristics of pesticide use. In addition, it also contributes to the gap in the literature regarding trace elements content in plasma and urine of workers exposed to pesticides.
Research, Society and Development
Pesquisas abordando o tema da violência autoinfligida são relevantes, mas têm sido pouco abordado... more Pesquisas abordando o tema da violência autoinfligida são relevantes, mas têm sido pouco abordados no Brasil. Este estudo tem como objetivo analisar dados sobre notificações de mortes por lesões autoprovocadas no estado do Paraná. Trata-se de um estudo ecológico, descritivo, de abordagem quantitativa a partir de dados secundários obtidos dos sistemas de informação de saúde nacionais de casos notificados de óbitos por lesão autoprovocada intencionalmente no estado do Paraná, no período de 2009 a 2018, referente ao Código Internacional da Doença (CID) 10 da categoria X60 a X84. Foram identificados 6609 óbitos por lesões autoprovocadas com crescimento significativo (p<0,0001) no período analisado, com predominância do sexo masculino (79,69%), solteiro (49,27%), com maior ocorrência em domicílio (64,26%) e faixa etária entre 20 a 59 anos. A principal causa de óbito foi a lesão autoprovocada por enforcamento, estrangulamento e sufocação. Autointoxicação por medicamentos e drogas de ab...
Saúde e Pesquisa
O objetivo desta pesquisa foi conhecer o consumo de álcool entre estudantes de uma universidade n... more O objetivo desta pesquisa foi conhecer o consumo de álcool entre estudantes de uma universidade no sul do Brasil. Realizou-se estudo transversal, quantitativo, com acadêmicos de Biomedicina (n=134) por meio do instrumento Alcohol Use Disorders Identification Test, versão traduzida. Utilizou-se teste estatístico para verificar associação entre variáveis categóricas. Houve predomínio de mulheres, de 18 a 21 anos. Verificou-se início precoce de consumo de álcool, anterior à chegada à universidade, vinculado a festas e companhia de amigos. Após ingressar no curso, 41,04% disseram ter aumentado a ingestão (p=0,0001). Quanto ao risco, 72,38% foram classificados como consumidores de baixo risco, 19,39% como de risco, 5,98% de alto risco e 2,25% dependentes. Ser acadêmico da área da saúde e conhecer os prejuízos associados ao consumo do álcool não favorece a adoção de um estilo de vida saudável. Evidencia-se que o uso de álcool não é exclusivamente influenciado pelo conhecimento dos riscos.
Food and Chemical Toxicology
This study aimed to evaluate the risk concerning child population's health because of the occ... more This study aimed to evaluate the risk concerning child population's health because of the occurrence of AFM1 in UHT milk, powdered milk (PM) and infant formulae (IF). Determination of AFM1 was performed in 60 samples and evaluation of the mycotoxin exposure was carried out through the determination of the estimated daily intake (EDI), whereas risk characterization was evaluated with the calculation of the risk of Hepatocellular Carcinoma (HCC) and the Margin of Exposure (MOE). AFM1 ranged from 150 to 1,020 ng/kg, and all the positive samples exceeded the limits stablished by European Community. The EDI for AFM1 ranged according to the age group of the population studied (0-5 years old) from 0.828-2.523, 0-2.113 and 0.029-0.833 ng/kg b.w./day in UHT, PM and IF, respectively. The number of HCC cases associated with AFM1 exposure (0.0015 a 0.0045) was higher than the limit of 0.001 case/100,000. MOE values for AFM1 were 728 to 239, considerably below the security margin of 10,000. These results point to a potential risk to the health of Brazilian child population exposed to AFM1 in dairy products.
Brazilian Journal of Pharmaceutical Sciences
Research, Society and Development
A exposição crônica aos agrotóxicos pode levar ao desenvolvimento de doenças, e a escassez de inf... more A exposição crônica aos agrotóxicos pode levar ao desenvolvimento de doenças, e a escassez de informações sobre manifestações clínicas na exposição a longo prazo é uma preocupação atual. Este trabalho teve como objetivo analisar o uso crônico de agrotóxicos da classe dos fungicidas por viticultores de um município do Paraná, região sul do Brasil. Foram entrevistados 192 agricultores familiares, que residiam em 66 propriedades, com instrumentos desenvolvidos segundo o protocolo de avaliação das intoxicações crônicas por agrotóxicos da Secretaria de Estado da Saúde do Paraná. Entre os participantes, 45,8% trabalhava há mais de 20 anos na viticultura, utilizando continuamente os fungicidas e apenas 6,8% relatou uso de todos os equipamentos de proteção recomendados para a aplicação dos agrotóxicos. Foram relatados sintomas como irritação cutânea pruriginosa (20,5%) e tremores (12,1%) que podem estar associados a exposição crônica aos fungicidas ditiocarbamatos. A população agricultora f...
Food Additives & Contaminants: Part A
ABSTRACT There is an increasing demand for fungi control in grains, especially toxigenic. Also, t... more ABSTRACT There is an increasing demand for fungi control in grains, especially toxigenic. Also, there is growing concern on the use of synthetic fungicides; thus alternatives are needed. The aim of this study was to evaluate the antifungal and antimycotoxigenic action of essential oils (EOs) from Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii against Fusarium verticillioides, a spoilage and toxigenic fungus. Essential oils were first chemically characterised by gas chromatography coupled to mass spectrometry, and their antioxidant potential was measured by the DPPH, ABTS and FRAP methods. Minimum inhibitory concentration (MIC) and disc diffusion were used to assess antifungal activity. Scanning electron microscopy was used to evaluate morphological changes in the fungus. Antimycotoxigenic activity of the EOs against the production of fumonisin B1 and B2 by F. verticillioides was evaluated using ultra-high-performance liquid chromatography system. Z. officinale, C. zeylanicum and C. martinii EOs were predominantly composed by zingiberene and geranial; eugenol; and geraniol, respectively. All the EOs had high antioxidant power, especially that from C. zeylanicum. The MICs were 250, 500 and 2,000 µg mL−1 for C. zeylanicum, C. martinii and Z. officinale EOs, respectively. Mycelial reduction of F. verticillioides was observed when EOs were used, and the lowest activity was detected in the Z. officinale EO. Overall, the tested EOs promoted structural damage to the fungal cell wall, decreased conidia size and mycelial reduction. Antimycotoxigenic evaluation of the EOs evidenced a significant reduction (p < .05) in the production of fumonisins B1 and B2 with all the EOs evaluated in the study. These results suggest that especially C. zeylanicum and C. martinii EOs are highly useful for controlling F. verticillioides and fumonisins production. GRAPHICAL ABSTRACT
Food Additives & Contaminants: Part A
ABSTRACT The increased risk to health by diverse pathologies, such as cancer, liver diseases, and... more ABSTRACT The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrine alterations, caused by chemical residues in food, has led to the search for sustainable agricultural management alternatives, such as the use of essential oils for the development of natural and eco-friendly fungicides. The aim of this study was to evaluate the antifungal and antiaflatoxigenic activity of Rosmarinus officinalis L. essential oil (REO) against Aspergillus flavus Link. REO was obtained by hydrodistillation and its major components were identified as 1,8-cineole (eucalyptol, 52.2%), camphor (15.2%) and α-pinene (12.4%) by GC/MS and NMR. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were both 500 µg/mL. REO reduced the mycelial growth of A. flavus at a concentration of 250 µg/mL (15.3%). The results obtained from scanning electron microscopy (SEM) demonstrated a reduction in the size of conidiophores and in the thickness of hyphae in A. flavus caused by treatment with REO (250 µg/mL). The production of ergosterol and the biomass of mycelium were both reduced as the REO treatment concentration increased. The production of aflatoxins B1 and B2 was inhibited after treatment with 250 µg/mL REO, a concentration below the MIC/MFC, indicating that the antiaflatoxigenic effect of REO is independent of its antifungal effect and is likely due to its direct action upon toxin biosynthesis. The data demonstrated that REO may be used as an alternative to synthetic fungicides. Graphical abstract
Journal of Food Safety
The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and c... more The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and commercialized in State of Parana, Brazil. The extraction method carried out with a Romer Mycosep 226 column was found to be efficient due to addition of both diatomaceous earth and centrifuging during the cleaning step. From the total of 84 analyzed samples, 78.6% (n = 66) were found to be contaminated ZEA at levels between 15.4 and 72.1 μg/kg. This study was the first report on occurrence of positive samples for ZEA in corn meal commercialized in Parana since 2005. The estimated daily intake (EDI) of ZEA for the Brazilian population and for the State of Parana was 3.1 and 2.2 ng/kg bw/day, respectively. The EDI was below the tolerable daily intake of 250 ng/kg bw/day. Despite the low health risk of the population, it was observed that the occurrence of ZEA in corn meal was frequent, which demonstrates the importance of monitoring studies to ensure consumer's health. PRACTICAL APPLICATIONS: Occurrence of zearalenone in corn meal was high (78.6%) in Brazil. First report on corn meal samples positive for zearalenone in Parana, Brazil. No sample exceeded the tolerated limit by both Brazilian and European legislation. EDI due to corn meal consumption was inferior to TDI.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 2018
Members of the Fusarium genus are capable of contaminating agricultural commodities, compromising... more Members of the Fusarium genus are capable of contaminating agricultural commodities, compromising the quality of maize and other grains, which leads to severe quality and yield losses. Contamination with mycotoxins is also a concern. Essential oils are possible alternatives to the use of synthetic pesticides for control of fungal contamination, as many have antifungal and anti-mycotoxigenic properties and are innocuous to human health. They also do not cause any sort of microbial resistance and do not promote environmental pollution. The aim of this study was to evaluate the antifungal and anti-mycotoxigenic effects of Zingiber officinale Roscoe essential oil (GEO) upon Fusarium graminearum Schwabe in vitro. The essential oil was extracted by hydrodistillation and analysed by GC/MS. Antifungal and anti-mycotoxigenic activities were assessed by HPLC/UV by quantifying ergosterol and deoxynivalenol (DON), respectively. Results indicated that GEO inhibited ergosterol production at a con...
Acta Farmaceutica Bonaerense, 2005
... heterocíclicas 100 . Folhas de Nim podem atuar como mediador na ativação da resposta imune. B... more ... heterocíclicas 100 . Folhas de Nim podem atuar como mediador na ativação da resposta imune. Baral &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Chatto-padhyay 75, estudando em ratos o efeito profilá-tico e terapêutico ... co ao homem. Kasturi et al. 117 verificaram ...