Simone Simionatto - Academia.edu (original) (raw)
Papers by Simone Simionatto
Revista do Instituto de Medicina Tropical de São Paulo
This is an open-access article distributed under the terms of the Creative Commons Attribution Li... more This is an open-access article distributed under the terms of the Creative Commons Attribution License.
BMC Proceedings, 2014
Background Reports of nosocomial infection due to carbapenem resistant Serratia spp. have become ... more Background Reports of nosocomial infection due to carbapenem resistant Serratia spp. have become significantly more common. This resistance may be due to production of distinct carbapenemases, such as KPC [1,2]. This enzyme, initially described in Klebsiella pneumoniae isolates, has also been detected among other organisms, such as Serratia marcescens, emphasizing the global risk of interspecies spread of resistance genes [2,3]. The aim of this study was to identify carbapenemase genes in Serratia spp. Isolated of nosocomial infection.
Scientific Data
The One Health concept is a global strategy to study the relationship between human and animal he... more The One Health concept is a global strategy to study the relationship between human and animal health and the transfer of pathogenic and non-pathogenic species between these systems. However, to the best of our knowledge, no data based on One Health genome-centric metagenomics are available in public repositories. Here, we present a dataset based on a pilot-study of 2,915 metagenome-assembled genomes (MAGs) of 107 samples from the human (N = 34), cattle (N = 28), swine (N = 15) and poultry (N = 30) gut microbiomes. Samples were collected from the five Brazilian geographical regions. Of the draft genomes, 1,273 were high-quality drafts (≥90% of completeness and ≤5% of contamination), and 1,642 were medium-quality drafts (≥50% of completeness and ≤10% of contamination). Taxonomic predictions were based on the alignment and concatenation of single-marker genes, and the most representative phyla were Bacteroidota, Firmicutes, and Proteobacteria. Many of these species represent potential...
Veterinary parasitology, Jan 19, 2011
Neosporosis is of alarming economic concern in the cattle industry. The effectiveness of diagnost... more Neosporosis is of alarming economic concern in the cattle industry. The effectiveness of diagnostic tests for detecting specific antibodies against Neospora caninum is hampered by potential cross-reaction with other coccidia. Use of a single specific antigen might improve test specificity. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using the truncated protein NcSRS2 expressed in Escherichia coli. The ELISA results were compared with those of the indirect fluorescence antibody test (IFAT). Receiver Operating Characteristic (ROC) and Tests in the Absence of a Gold Standard (TAGS) analysis revealed an assay having 96% specificity and 95% sensitivity when applied to 145 positive and 352 negative sera from two distinct cattle populations. Using OD ≤ 0.095 as the cut-off point, the assay's negative and positive predictive values ranged from 98.8% to 50.8% and from 58.8% to 99.1%, respectively, depending on neosporosis prevalence in a given area. The novel ELIS...
Revista do Instituto de Medicina Tropical de São Paulo
This is an open-access article distributed under the terms of the Creative Commons Attribution Li... more This is an open-access article distributed under the terms of the Creative Commons Attribution License.
BMC Proceedings, 2014
Background Reports of nosocomial infection due to carbapenem resistant Serratia spp. have become ... more Background Reports of nosocomial infection due to carbapenem resistant Serratia spp. have become significantly more common. This resistance may be due to production of distinct carbapenemases, such as KPC [1,2]. This enzyme, initially described in Klebsiella pneumoniae isolates, has also been detected among other organisms, such as Serratia marcescens, emphasizing the global risk of interspecies spread of resistance genes [2,3]. The aim of this study was to identify carbapenemase genes in Serratia spp. Isolated of nosocomial infection.
Scientific Data
The One Health concept is a global strategy to study the relationship between human and animal he... more The One Health concept is a global strategy to study the relationship between human and animal health and the transfer of pathogenic and non-pathogenic species between these systems. However, to the best of our knowledge, no data based on One Health genome-centric metagenomics are available in public repositories. Here, we present a dataset based on a pilot-study of 2,915 metagenome-assembled genomes (MAGs) of 107 samples from the human (N = 34), cattle (N = 28), swine (N = 15) and poultry (N = 30) gut microbiomes. Samples were collected from the five Brazilian geographical regions. Of the draft genomes, 1,273 were high-quality drafts (≥90% of completeness and ≤5% of contamination), and 1,642 were medium-quality drafts (≥50% of completeness and ≤10% of contamination). Taxonomic predictions were based on the alignment and concatenation of single-marker genes, and the most representative phyla were Bacteroidota, Firmicutes, and Proteobacteria. Many of these species represent potential...
Veterinary parasitology, Jan 19, 2011
Neosporosis is of alarming economic concern in the cattle industry. The effectiveness of diagnost... more Neosporosis is of alarming economic concern in the cattle industry. The effectiveness of diagnostic tests for detecting specific antibodies against Neospora caninum is hampered by potential cross-reaction with other coccidia. Use of a single specific antigen might improve test specificity. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using the truncated protein NcSRS2 expressed in Escherichia coli. The ELISA results were compared with those of the indirect fluorescence antibody test (IFAT). Receiver Operating Characteristic (ROC) and Tests in the Absence of a Gold Standard (TAGS) analysis revealed an assay having 96% specificity and 95% sensitivity when applied to 145 positive and 352 negative sera from two distinct cattle populations. Using OD ≤ 0.095 as the cut-off point, the assay's negative and positive predictive values ranged from 98.8% to 50.8% and from 58.8% to 99.1%, respectively, depending on neosporosis prevalence in a given area. The novel ELIS...