Sita Gaffar - Academia.edu (original) (raw)

Papers by Sita Gaffar

Research paper thumbnail of Covid-19 Infection in a Teenage Patient with Nasopharyngeal Cancer

Advances in Health Sciences Research, 2021

Research paper thumbnail of Surgical Management of Tympanojugulare Paragangliomas: A Rare and Challenging Case of Vascular Neoplasma

Advances in Health Sciences Research, 2021

Research paper thumbnail of NPC-Exosome Carry Wild and Mutant-type p53 among Nasopharyngeal Cancer Patients

The Indonesian Biomedical Journal

BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exoso... more BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exosome (NPC-Exo) could carry p53. However, information regarding the type of p53 carrier on NPC-Exo remains limited. This study aims to introduce our important findings regarding the type of p53 NPC-Exo cargo.METHODS: Serum from patients with NPC were prepared for exosome isolation with Seramir Exoquick by following the manual instructions. RT-PCR was conducted to determine the expression levels of latent membrane protein 1 (LMP-1) and p53 in the exosome isolate. Partial sequencing of p53 amplicon was conducted to determine mutation type of p53.RESULTS: There were 8 patients enrolled in this study. According to RT-PCR results, the expression levels of LMP-1 and p53 varied in the NPC-Exo isolate. Based on sequencing analysis, 1 case of p53 mutation was noticeable.CONCLUSION: According to current results, the NPC-derived exosome potentially carries not only wild type but also mutant type p53. ...

Research paper thumbnail of 1874-4281/09 2009 Bentham Open Open Access A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis of Connexin 26 (GJB2) Gene Common Mutation (235delC) In Indonesian Patients with Prelingual Nonsyndromic Sensorineural Hear

Abstract: Background: Inherited hearing impairment affects about 1 in 1000 newborns. Up to 50 per... more Abstract: Background: Inherited hearing impairment affects about 1 in 1000 newborns. Up to 50 percent of all patients with autosomal recessive nonsyndromic prelingual deafness in many populations have mutations in the gene encoding the gap junction protein connexin 26 (GJB2) at locus DFNB1 (autosomal recessive nonsyndromic deafness) on chromosome 13q11-12. In East Asia, there is a common mutation (235delC) of connexin 26 (GJB2) gene mutation, and a common GJB2 gene mutation (V37I) in Singapore and Malaysia with congenital deafness. No connexin 26 gene study was done in Indonesia. In this preliminary study, we analyzed 40 Indonesian patients with prelingual nonsyndromic sensorineural hearing loss. Objective: To detect the common frameshift mutation (235delC) of connexin 26 (GJB2) gene in Indonesian patients by using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) analysis. Materials and Methods: Forty patients with prelingual nonsyndromic sensorineur...

Research paper thumbnail of NPC-Exosome Carry Wild and Mutant-type p53 among Nasopharyngeal Cancer Patients

The Indonesian Biomedical Journal

BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exoso... more BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exosome (NPC-Exo) could carry p53. However, information regarding the type of p53 carrier on NPC-Exo remains limited. This study aims to introduce our important findings regarding the type of p53 NPC-Exo cargo.METHODS: Serum from patients with NPC were prepared for exosome isolation with Seramir Exoquick by following the manual instructions. RT-PCR was conducted to determine the expression levels of latent membrane protein 1 (LMP-1) and p53 in the exosome isolate. Partial sequencing of p53 amplicon was conducted to determine mutation type of p53.RESULTS: There were 8 patients enrolled in this study. According to RT-PCR results, the expression levels of LMP-1 and p53 varied in the NPC-Exo isolate. Based on sequencing analysis, 1 case of p53 mutation was noticeable.CONCLUSION: According to current results, the NPC-derived exosome potentially carries not only wild type but also mutant type p53. ...

Research paper thumbnail of Bilateral osteoradionecrosis of the temporal bones: a rare complication of radiotherapy

Osteoradionecrosis of the temporal bone is a rare complication of radiotherapy treatment for naso... more Osteoradionecrosis of the temporal bone is a rare complication of radiotherapy treatment for nasopharyngeal carcinoma (NPC). We report the case of a 72-year-old man with osteoradionecrosis of bilateral temporal bones three years after completion of radiation therapy for NPC. He presented to us with chronic ear discharge secondary to persistent right chronic otitis media for the past 15 years. Pure tone audiometry showed bilateral moderate mixed hearing loss. A right cortical mastoidectomy showed extensive granulation tissue in the mastoid cavity. Recovery was uneventful but required the use of hearing aids.

Research paper thumbnail of Anatomical Measurement of Normal Eustachian Tube on the Temporal Bone Computed Tomography Imaging

Tujuan: Menggambarkan pengukuran anatomis tuba Eustachius normal pada pencitraan Computed Tomogra... more Tujuan: Menggambarkan pengukuran anatomis tuba Eustachius normal pada pencitraan Computed Tomography (CT) tulang temporal. Metode: Ini adalah penelitian deskriptif tuba Eustachius normal dengan mengukur diameter pretimpani, sudut tubotimpani dan panjang pars kartilago tuba Eustachius normal menggunakan teknik rekonstruksi multiplanar pada pencitraan CT tulang temporal dari 58 sampel di beberapa rumah sakit di Makassar. Semua sampel dibagi lagi menjadi kelompok laki-laki dan perempuan, serta kelompok sisi kanan dan kiri. Kami juga membagi sampel menjadi 6 kelompok usia. Hasil: Rerata diameter pretimpani pada kelompok pria adalah 4,62 mm dan pada kelompok wanita adalah 4,36 mm. Rerata diameter pretimpani adalah 4,59 mm pada kelompok sisi kanan dan 4,36 mm pada kelompok sisi kiri. Rerata sudut tubotimpani adalah 143,5o pada kelompok pria dan 143,3o pada kelompok wanita. Nilai rerata sudut tubotimpani tidak memiliki perbedaan di kedua sisi telinga. Rerata panjang pars kartilago tidak ...

Research paper thumbnail of The Comparison of Exosome RNA Concentration and its LMP-1 and p53 content Among Patients with Nasopharyngeal Cancer before and after Chemoradiation

ObjectiveNasopharyngeal cancer (NPC) remains the highest head and neck malignancy in Asia, especi... more ObjectiveNasopharyngeal cancer (NPC) remains the highest head and neck malignancy in Asia, especially in Southeast Asia. The exosome is a nanocarrier that has a role in inhibit or promote the progression of cancer as well as the marker to knows the response of therapy depending on its carrier inside. NPC-exosome carriers including LMP-1 and p53. LMP-1 is a main oncogene of Epstein Barr Virus (EBV) the predominant cause of NPC. Wild type p53 known as the main inhibitor of cancer growth. The study aims to compare the concentration of exosomes and the characteristic of LMP-1 and p53 inside exosomes among patients with NPCResultEight patients fulfilled the study criteria. The mean age was 37,5 years old (SD = 9,9), most of them were stage III. The mean exosome RNA concentration before and after chemoradiation was 14,39 and 3,61 ng/ml with a p-value of 0,04. LMP-1 expression was documented inside the exosome. Expression of p53 in exon 4-5 and 5-6 are express well, except on exon 7-8. Fur...

Research paper thumbnail of The potential role of exosome on cytokine storm and treatment of severe COVID-19 infection

Bali Medical Journal, 2020

The symptoms of COVID-19 varies from mild to severe The risk of severe infection occurs about 10%... more The symptoms of COVID-19 varies from mild to severe The risk of severe infection occurs about 10% of cases, while the risk of death occurs about 0-14 6% of cases One of the suspected pathophysiology in cases of severe infection and death of COVID-19 is due to cytokine storms Exosome plays a vital role in the pathogenesis of several diseases, including severe disease such as sepsis due to cytokine storm Exosome has been proven as a nanomaterial carrier that can tackle cytokine storm in the treatment of severe COVID-19 infection Several studies have been conducted for this purpose, and its clinical application continually increases This review will explore the role of exosomes in cytokine storms that occur in patients with COVID-19 and seek the opportunity to use exosomes in their management © 2020, Sanglah General Hospital All rights reserved

Research paper thumbnail of A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis of Connexin 26 (GJB2) Gene Common Mutation (235delC) In Indonesian Patients with Prelingual Nonsyndromic Sensorineural Hearing Loss: A Preliminary Study

The Open Otorhinolaryngology Journal, 2009

Research paper thumbnail of Akurasi Gambaran CT Scan Tulang Temporal Preoperatif dalam Menilai Kolesteatoma pada Penderita Otitis Media Supuratif Kronis (OMSK)

e-CliniC

This study was aimed to determine the accuracy of preoperative temporal bone CTscan ini assessing... more This study was aimed to determine the accuracy of preoperative temporal bone CTscan ini assessing cholesteatoma in chronic suppurative otitis media (CSOM) patients. This was a diagnostic test study conducted by comparing the findings of the preoperative temporal bone CTscan with the intraoperative findings of 54 CSOM patients who had a temporal bone CT-scan followed by surgery at the Hasanuddin University Hospital and the Jaury Academic Hospital. Assessment of cholesteatoma on a preoperative temporal bone CT-scan was performed when soft tissue density was found in the middle ear accompanied by bone erosion. In addition, an assessment was also carried out for the presence of ossicular, scutum, tympanic tegmen, facial nerve canal and mastoid tegmen erosions. The results indicated that the accuracy of preoperative temporal bone CT-scan in assessing cholesteatoma in CSOM patients was 87.04% with a sensitivity of 85%, specificity of 88.23%, a positive predictive value of 80.95%, and a negative predictive value of 90.91%. The sensitivity of the preoperative temporal bone CT-scan in assessing the highest erosion of cholesteatoma in the erosion of the scutum and tympanic tegmen (100%) with the specificity and accuracy of the preoperative temporal bone CT scan of the in assessing erosions in cholesteatoma highest on mastoid tegman erosion (100% and 96.29%). In conclusion, preoperative CT scan of temporal bone has high accuracy, sensitivity, and specifity values in assessing cholesteatoma and erosions of surrounding structures. Keywords: cholesteatoma, chronic suppurative otitis media (CSOM), temporal bone CT-Scan Abstrak: Penelitian ini bertujuan untuk mengetahui akurasi gambaran CT-scan tulang temporal preoperatif dalam menilai kolesteatoma pada penderita otitis media supuratif kronis (OMSK). Jenis penelitian ialah uji diagnostik yang membandingkan temuan pada CT scan tulang temporal preoperatif dengan hasil temuan intraoperatif pada 54 penderita OMSK yang menjalani pemeriksaan CT scan tulang temporal dilanjutkan dengan tindakan operasi di Rumah Sakit Universitas Hasanuddin dan Rumah Sakit Akademis Jaury. Penilaian kolesteatoma pada CT Scan tulang temporal preoperatif ketika ditemukan densitas jaringan lunak di telinga tengah yang disertai dengan erosi tulang. Selain itu, dilakukan penilaian adanya erosi osikula, skutum, tegmen timpani, kanalis nervus fasialis, dan tegmen mastoid. Hasil penelitian mendapatkan akurasi CT scan tulang temporal preoperatif dalam menilai kolesteatoma pada penderita OMSK sebesar 87,04% dengan sensitivitas 85%, spesifisitas 88,23%, nilai prediksi positif 80,95%, dan nilai prediksi negatif 90,91%. Sensitivitas CT scan tulang temporal preoperatif dalam menilai erosi pada kolesteatoma tertinggi pada erosi skutum dan tegmen timpani (100%) dengan spesifisitas dan akurasi CT scan tulang temporal preoperatif dalam menilai erosi pada kolesteatoma tertinggi pada erosi tegmen mastoid (100% dan 96.29%). Simpulan penelitian ini ialah CT scan tulang temporal preoperatif memiliki nilai akurasi, sensitivitas, serta spesifisitas yang cukup tinggi dalam menilai kolesteatoma serta erosi pada struktur di sekitarnya. Kata kunci: kolesteatoma, otitis media supuratif kronis (OMSK), CT scan tulang temporal

Research paper thumbnail of Deteksi pepsin pada penderita refluks laringofaring yang didiagnosis berdasarkan reflux symptom index dan reflux finding score

Oto Rhino Laryngologica Indonesiana

Background: It is estimated that more than 50% of patients with voice disorders who come fortreat... more Background: It is estimated that more than 50% of patients with voice disorders who come fortreatment are caused by laryngopharyngeal reflux (LPR). LPR has been implicated in the etiology ofmany laryngeal disorders including subglottic stenosis, laryngeal carcinoma, laryngeal contact ulcer,laryngospasm and vocal nodule on the vocal cords. Ambulatory 24 hour double-probe (pharyngeal andesophageal) pH monitoring is the gold standard examination for diagnosing LPR, but it is still far fromideal criteria.The assessment of pepsin in airway secretions could be used as a sensitive diagnosticmarker of LPR because pepsin is not synthesized by any type of airway cells. Purpose: The aim ofthis study was to detect the presence of pepsin on laryingopharyngeal reflux patients which diagnosedbased on reflux symptom index (RSI) dan reflux finding score (RFS) at Wahidin Sudirohusodo Hospital,Makassar. Methods: This is a comparative quantitative study. We performed RSI and RFS examinationson 51 sampl...

Research paper thumbnail of Incidence and characteristics of anemia among patients with nasopharyngeal carcinoma in Lombok, Indonesia

PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019

Since the anemia and nasopharyngeal cancer (NPC) are a major problem in Indonesia and anemia has ... more Since the anemia and nasopharyngeal cancer (NPC) are a major problem in Indonesia and anemia has become one of the prognostic factors for the treatment of NPC, it is important to analyse the incidence and its characteristics in NPC patients especially in rural areas in Indonesia, such as Lombok. The purpose of this study is to analyse the incidence and the characteristics of anemia in NPC patients in a secondary referral hospital, Lombok, Indonesia. This research was carried out by analytic descriptive study using medical record data from secondary referral hospitals, Lombok, Indonesia. The data were obtained between April 2017 to March 2018. Nasopharyngeal cancer was established through physical examination, imaging, and histopathological examination. The stage of disease was defined according to AJCC 2017. Anemia was defined as Haemoglobin level less than 11 g/dl and the severity level according to WHO criteria. Classification of anemia based on MCV and MCHC are divided into normocytic, microcytic and macrocytic as well as normochromic, hypochromic, and hyperchromic according to the criteria of Chinese Clinical Oncology guideline 2012. There were 40 cases of patient with anemia who fulfil the study criteria, which consist of 30 male and 10 female, with the ratio 3: 1. According to the age, patients were distributed from 13 to 67 years old with the mean age for males was 46 (SD± 11.04) years old while the mean age for female was 50,9 (SD± 12,37) years old. Patients were found in stage II-IV, however, most of them were stage IV (68.75%). There were 87.5% of patients with anemia in different severity levels. However, the highest number was found in mild anemia level (57.5%). Based on the erythrocyte index, the majority types of anemia were hypochromic microcytic and normocytic normochromic (39,3%). The incidence of anemia in a patient with nasopharyngeal carcinoma at secondary referral hospitals in Lombok was 87,5%. Most of them were mild and hypochromic microcytic as well as normocytic normochromic type.

Research paper thumbnail of Acute MRSA rhinosinusitis with periorbital cellulitis: A case report

Journal of Otology & Rhinology

Research paper thumbnail of Covid-19 Infection in a Teenage Patient with Nasopharyngeal Cancer

Advances in Health Sciences Research, 2021

Research paper thumbnail of Surgical Management of Tympanojugulare Paragangliomas: A Rare and Challenging Case of Vascular Neoplasma

Advances in Health Sciences Research, 2021

Research paper thumbnail of NPC-Exosome Carry Wild and Mutant-type p53 among Nasopharyngeal Cancer Patients

The Indonesian Biomedical Journal

BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exoso... more BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exosome (NPC-Exo) could carry p53. However, information regarding the type of p53 carrier on NPC-Exo remains limited. This study aims to introduce our important findings regarding the type of p53 NPC-Exo cargo.METHODS: Serum from patients with NPC were prepared for exosome isolation with Seramir Exoquick by following the manual instructions. RT-PCR was conducted to determine the expression levels of latent membrane protein 1 (LMP-1) and p53 in the exosome isolate. Partial sequencing of p53 amplicon was conducted to determine mutation type of p53.RESULTS: There were 8 patients enrolled in this study. According to RT-PCR results, the expression levels of LMP-1 and p53 varied in the NPC-Exo isolate. Based on sequencing analysis, 1 case of p53 mutation was noticeable.CONCLUSION: According to current results, the NPC-derived exosome potentially carries not only wild type but also mutant type p53. ...

Research paper thumbnail of 1874-4281/09 2009 Bentham Open Open Access A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis of Connexin 26 (GJB2) Gene Common Mutation (235delC) In Indonesian Patients with Prelingual Nonsyndromic Sensorineural Hear

Abstract: Background: Inherited hearing impairment affects about 1 in 1000 newborns. Up to 50 per... more Abstract: Background: Inherited hearing impairment affects about 1 in 1000 newborns. Up to 50 percent of all patients with autosomal recessive nonsyndromic prelingual deafness in many populations have mutations in the gene encoding the gap junction protein connexin 26 (GJB2) at locus DFNB1 (autosomal recessive nonsyndromic deafness) on chromosome 13q11-12. In East Asia, there is a common mutation (235delC) of connexin 26 (GJB2) gene mutation, and a common GJB2 gene mutation (V37I) in Singapore and Malaysia with congenital deafness. No connexin 26 gene study was done in Indonesia. In this preliminary study, we analyzed 40 Indonesian patients with prelingual nonsyndromic sensorineural hearing loss. Objective: To detect the common frameshift mutation (235delC) of connexin 26 (GJB2) gene in Indonesian patients by using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) analysis. Materials and Methods: Forty patients with prelingual nonsyndromic sensorineur...

Research paper thumbnail of NPC-Exosome Carry Wild and Mutant-type p53 among Nasopharyngeal Cancer Patients

The Indonesian Biomedical Journal

BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exoso... more BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exosome (NPC-Exo) could carry p53. However, information regarding the type of p53 carrier on NPC-Exo remains limited. This study aims to introduce our important findings regarding the type of p53 NPC-Exo cargo.METHODS: Serum from patients with NPC were prepared for exosome isolation with Seramir Exoquick by following the manual instructions. RT-PCR was conducted to determine the expression levels of latent membrane protein 1 (LMP-1) and p53 in the exosome isolate. Partial sequencing of p53 amplicon was conducted to determine mutation type of p53.RESULTS: There were 8 patients enrolled in this study. According to RT-PCR results, the expression levels of LMP-1 and p53 varied in the NPC-Exo isolate. Based on sequencing analysis, 1 case of p53 mutation was noticeable.CONCLUSION: According to current results, the NPC-derived exosome potentially carries not only wild type but also mutant type p53. ...

Research paper thumbnail of Bilateral osteoradionecrosis of the temporal bones: a rare complication of radiotherapy

Osteoradionecrosis of the temporal bone is a rare complication of radiotherapy treatment for naso... more Osteoradionecrosis of the temporal bone is a rare complication of radiotherapy treatment for nasopharyngeal carcinoma (NPC). We report the case of a 72-year-old man with osteoradionecrosis of bilateral temporal bones three years after completion of radiation therapy for NPC. He presented to us with chronic ear discharge secondary to persistent right chronic otitis media for the past 15 years. Pure tone audiometry showed bilateral moderate mixed hearing loss. A right cortical mastoidectomy showed extensive granulation tissue in the mastoid cavity. Recovery was uneventful but required the use of hearing aids.

Research paper thumbnail of Anatomical Measurement of Normal Eustachian Tube on the Temporal Bone Computed Tomography Imaging

Tujuan: Menggambarkan pengukuran anatomis tuba Eustachius normal pada pencitraan Computed Tomogra... more Tujuan: Menggambarkan pengukuran anatomis tuba Eustachius normal pada pencitraan Computed Tomography (CT) tulang temporal. Metode: Ini adalah penelitian deskriptif tuba Eustachius normal dengan mengukur diameter pretimpani, sudut tubotimpani dan panjang pars kartilago tuba Eustachius normal menggunakan teknik rekonstruksi multiplanar pada pencitraan CT tulang temporal dari 58 sampel di beberapa rumah sakit di Makassar. Semua sampel dibagi lagi menjadi kelompok laki-laki dan perempuan, serta kelompok sisi kanan dan kiri. Kami juga membagi sampel menjadi 6 kelompok usia. Hasil: Rerata diameter pretimpani pada kelompok pria adalah 4,62 mm dan pada kelompok wanita adalah 4,36 mm. Rerata diameter pretimpani adalah 4,59 mm pada kelompok sisi kanan dan 4,36 mm pada kelompok sisi kiri. Rerata sudut tubotimpani adalah 143,5o pada kelompok pria dan 143,3o pada kelompok wanita. Nilai rerata sudut tubotimpani tidak memiliki perbedaan di kedua sisi telinga. Rerata panjang pars kartilago tidak ...

Research paper thumbnail of The Comparison of Exosome RNA Concentration and its LMP-1 and p53 content Among Patients with Nasopharyngeal Cancer before and after Chemoradiation

ObjectiveNasopharyngeal cancer (NPC) remains the highest head and neck malignancy in Asia, especi... more ObjectiveNasopharyngeal cancer (NPC) remains the highest head and neck malignancy in Asia, especially in Southeast Asia. The exosome is a nanocarrier that has a role in inhibit or promote the progression of cancer as well as the marker to knows the response of therapy depending on its carrier inside. NPC-exosome carriers including LMP-1 and p53. LMP-1 is a main oncogene of Epstein Barr Virus (EBV) the predominant cause of NPC. Wild type p53 known as the main inhibitor of cancer growth. The study aims to compare the concentration of exosomes and the characteristic of LMP-1 and p53 inside exosomes among patients with NPCResultEight patients fulfilled the study criteria. The mean age was 37,5 years old (SD = 9,9), most of them were stage III. The mean exosome RNA concentration before and after chemoradiation was 14,39 and 3,61 ng/ml with a p-value of 0,04. LMP-1 expression was documented inside the exosome. Expression of p53 in exon 4-5 and 5-6 are express well, except on exon 7-8. Fur...

Research paper thumbnail of The potential role of exosome on cytokine storm and treatment of severe COVID-19 infection

Bali Medical Journal, 2020

The symptoms of COVID-19 varies from mild to severe The risk of severe infection occurs about 10%... more The symptoms of COVID-19 varies from mild to severe The risk of severe infection occurs about 10% of cases, while the risk of death occurs about 0-14 6% of cases One of the suspected pathophysiology in cases of severe infection and death of COVID-19 is due to cytokine storms Exosome plays a vital role in the pathogenesis of several diseases, including severe disease such as sepsis due to cytokine storm Exosome has been proven as a nanomaterial carrier that can tackle cytokine storm in the treatment of severe COVID-19 infection Several studies have been conducted for this purpose, and its clinical application continually increases This review will explore the role of exosomes in cytokine storms that occur in patients with COVID-19 and seek the opportunity to use exosomes in their management © 2020, Sanglah General Hospital All rights reserved

Research paper thumbnail of A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis of Connexin 26 (GJB2) Gene Common Mutation (235delC) In Indonesian Patients with Prelingual Nonsyndromic Sensorineural Hearing Loss: A Preliminary Study

The Open Otorhinolaryngology Journal, 2009

Research paper thumbnail of Akurasi Gambaran CT Scan Tulang Temporal Preoperatif dalam Menilai Kolesteatoma pada Penderita Otitis Media Supuratif Kronis (OMSK)

e-CliniC

This study was aimed to determine the accuracy of preoperative temporal bone CTscan ini assessing... more This study was aimed to determine the accuracy of preoperative temporal bone CTscan ini assessing cholesteatoma in chronic suppurative otitis media (CSOM) patients. This was a diagnostic test study conducted by comparing the findings of the preoperative temporal bone CTscan with the intraoperative findings of 54 CSOM patients who had a temporal bone CT-scan followed by surgery at the Hasanuddin University Hospital and the Jaury Academic Hospital. Assessment of cholesteatoma on a preoperative temporal bone CT-scan was performed when soft tissue density was found in the middle ear accompanied by bone erosion. In addition, an assessment was also carried out for the presence of ossicular, scutum, tympanic tegmen, facial nerve canal and mastoid tegmen erosions. The results indicated that the accuracy of preoperative temporal bone CT-scan in assessing cholesteatoma in CSOM patients was 87.04% with a sensitivity of 85%, specificity of 88.23%, a positive predictive value of 80.95%, and a negative predictive value of 90.91%. The sensitivity of the preoperative temporal bone CT-scan in assessing the highest erosion of cholesteatoma in the erosion of the scutum and tympanic tegmen (100%) with the specificity and accuracy of the preoperative temporal bone CT scan of the in assessing erosions in cholesteatoma highest on mastoid tegman erosion (100% and 96.29%). In conclusion, preoperative CT scan of temporal bone has high accuracy, sensitivity, and specifity values in assessing cholesteatoma and erosions of surrounding structures. Keywords: cholesteatoma, chronic suppurative otitis media (CSOM), temporal bone CT-Scan Abstrak: Penelitian ini bertujuan untuk mengetahui akurasi gambaran CT-scan tulang temporal preoperatif dalam menilai kolesteatoma pada penderita otitis media supuratif kronis (OMSK). Jenis penelitian ialah uji diagnostik yang membandingkan temuan pada CT scan tulang temporal preoperatif dengan hasil temuan intraoperatif pada 54 penderita OMSK yang menjalani pemeriksaan CT scan tulang temporal dilanjutkan dengan tindakan operasi di Rumah Sakit Universitas Hasanuddin dan Rumah Sakit Akademis Jaury. Penilaian kolesteatoma pada CT Scan tulang temporal preoperatif ketika ditemukan densitas jaringan lunak di telinga tengah yang disertai dengan erosi tulang. Selain itu, dilakukan penilaian adanya erosi osikula, skutum, tegmen timpani, kanalis nervus fasialis, dan tegmen mastoid. Hasil penelitian mendapatkan akurasi CT scan tulang temporal preoperatif dalam menilai kolesteatoma pada penderita OMSK sebesar 87,04% dengan sensitivitas 85%, spesifisitas 88,23%, nilai prediksi positif 80,95%, dan nilai prediksi negatif 90,91%. Sensitivitas CT scan tulang temporal preoperatif dalam menilai erosi pada kolesteatoma tertinggi pada erosi skutum dan tegmen timpani (100%) dengan spesifisitas dan akurasi CT scan tulang temporal preoperatif dalam menilai erosi pada kolesteatoma tertinggi pada erosi tegmen mastoid (100% dan 96.29%). Simpulan penelitian ini ialah CT scan tulang temporal preoperatif memiliki nilai akurasi, sensitivitas, serta spesifisitas yang cukup tinggi dalam menilai kolesteatoma serta erosi pada struktur di sekitarnya. Kata kunci: kolesteatoma, otitis media supuratif kronis (OMSK), CT scan tulang temporal

Research paper thumbnail of Deteksi pepsin pada penderita refluks laringofaring yang didiagnosis berdasarkan reflux symptom index dan reflux finding score

Oto Rhino Laryngologica Indonesiana

Background: It is estimated that more than 50% of patients with voice disorders who come fortreat... more Background: It is estimated that more than 50% of patients with voice disorders who come fortreatment are caused by laryngopharyngeal reflux (LPR). LPR has been implicated in the etiology ofmany laryngeal disorders including subglottic stenosis, laryngeal carcinoma, laryngeal contact ulcer,laryngospasm and vocal nodule on the vocal cords. Ambulatory 24 hour double-probe (pharyngeal andesophageal) pH monitoring is the gold standard examination for diagnosing LPR, but it is still far fromideal criteria.The assessment of pepsin in airway secretions could be used as a sensitive diagnosticmarker of LPR because pepsin is not synthesized by any type of airway cells. Purpose: The aim ofthis study was to detect the presence of pepsin on laryingopharyngeal reflux patients which diagnosedbased on reflux symptom index (RSI) dan reflux finding score (RFS) at Wahidin Sudirohusodo Hospital,Makassar. Methods: This is a comparative quantitative study. We performed RSI and RFS examinationson 51 sampl...

Research paper thumbnail of Incidence and characteristics of anemia among patients with nasopharyngeal carcinoma in Lombok, Indonesia

PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019

Since the anemia and nasopharyngeal cancer (NPC) are a major problem in Indonesia and anemia has ... more Since the anemia and nasopharyngeal cancer (NPC) are a major problem in Indonesia and anemia has become one of the prognostic factors for the treatment of NPC, it is important to analyse the incidence and its characteristics in NPC patients especially in rural areas in Indonesia, such as Lombok. The purpose of this study is to analyse the incidence and the characteristics of anemia in NPC patients in a secondary referral hospital, Lombok, Indonesia. This research was carried out by analytic descriptive study using medical record data from secondary referral hospitals, Lombok, Indonesia. The data were obtained between April 2017 to March 2018. Nasopharyngeal cancer was established through physical examination, imaging, and histopathological examination. The stage of disease was defined according to AJCC 2017. Anemia was defined as Haemoglobin level less than 11 g/dl and the severity level according to WHO criteria. Classification of anemia based on MCV and MCHC are divided into normocytic, microcytic and macrocytic as well as normochromic, hypochromic, and hyperchromic according to the criteria of Chinese Clinical Oncology guideline 2012. There were 40 cases of patient with anemia who fulfil the study criteria, which consist of 30 male and 10 female, with the ratio 3: 1. According to the age, patients were distributed from 13 to 67 years old with the mean age for males was 46 (SD± 11.04) years old while the mean age for female was 50,9 (SD± 12,37) years old. Patients were found in stage II-IV, however, most of them were stage IV (68.75%). There were 87.5% of patients with anemia in different severity levels. However, the highest number was found in mild anemia level (57.5%). Based on the erythrocyte index, the majority types of anemia were hypochromic microcytic and normocytic normochromic (39,3%). The incidence of anemia in a patient with nasopharyngeal carcinoma at secondary referral hospitals in Lombok was 87,5%. Most of them were mild and hypochromic microcytic as well as normocytic normochromic type.

Research paper thumbnail of Acute MRSA rhinosinusitis with periorbital cellulitis: A case report

Journal of Otology & Rhinology