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Papers by Sitapha Ouattara
Parmi les maladies opportunistes, les aspergilloses, mycoses de l’appareil respiratoire essentiel... more Parmi les maladies opportunistes, les aspergilloses, mycoses de l’appareil respiratoire essentiellement de l’homme et des animaux, sont causees par des champignons du genre Aspergillus [1]. Pour apporter notre contribution a l’eradication de ces mycoses nous avons teste l’efficacite des residus de Terminalia ivorensis A.Chev. (une combretacee) sur la croissance in vitro de Aspergillus fumigatus. Les residus ont ete incorpores a la gelose sabouraud selon la methode de la double dilution en tubes penches. Nous avons ensemence 1000 cellules de Aspergillus fumigatus sur ces milieux. Les cultures ont ete incubees a 30°C pendant 48 heures. Les resultats des tests antifongiques montrent que parmi les dix residus testes, le residu non hexanosoluble X42 (CMF = 40 microg/ml ; CI50 = 4,56 microg/ml) est le plus actif. Par contre le residu aqueux Xaq (CMF = 190 microg/ml ; CI50 = 39,60 microg/ml) est le moins actif. L’utilisation d’un solvant comportant 70% d’ethanol et 30% d’eau suivie ...
The Journal of Phytopharmacology, 2016
Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regula... more Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regularly evaluated in different Laboratories. And, the processes of evaluation of antifungal susceptibility are often different to one laboratory to another. This study was undertaken in the goal to compare parameters obtained (MIC and MFC) with two antifungal susceptibility testing. The first one is Agar slant double dilution tubes method (M1) and the second is disc diffusion method /broth dilution according document CLSI M38 -A2 supplemented to 2% glucose (M2). Ethanol (EtOH 70%), Methanol (MeOH) and n-Hexane (n-HexTG) extracts of leaves of Tectona grandis have been tested in comparison to Itraconazole against two clinicals strains of Aspergillus fumigatus 896/AB and Aspergillus flavus 1006/OM isolated to HIV patients. Results showed that on A. flavus, agar slant method (M1) gave MIC ranging between 200 µg/mL and 800 µg/mL while disc diffusion meth/broth dilution (M2) showed growth inhibiti...
European Scientific Journal, ESJ, 2018
Introduction: Ocimum gratissimum is used in traditional Ivorian medicine for its multiple therape... more Introduction: Ocimum gratissimum is used in traditional Ivorian medicine for its multiple therapeutic virtues. Scope: Evaluate the biological properties of the essential oil of this plant. Methods: Antitumor activity was assessed using the MTS colorimetric assay on six (06) cell lines, the antifungal activity was evaluated by double dilution slant method on four (04) strains of clinical fungi, the potential antioxidant was determined by the DPPH test. Results: The strongest cytotoxic activity was obtained on Jurkat (T lymphoma) with an IC50 = 80 μg / ml. The best antifungal activity was observed on C. albicans with MIC and FMC values obtained at 12.5 μg / ml. Antioxidant activity was evaluated at F = 187 ± 1.57 mM Trolox / ml or (I = 38 ± 0.74%). Conclusion: The essential oil has interesting biological properties like as cytotoxic, antifungal and antioxidant
Journal of Drug Delivery and Therapeutics, 2013
Journal of Intercultural Ethnopharmacology, 2013
Aim: The present study was undertaken to evaluate in vitro antifungal activity of aqueous and hyd... more Aim: The present study was undertaken to evaluate in vitro antifungal activity of aqueous and hydroacoholic extracts from bark of Terminalia ivorensis A. Chev. (Combretaceae). Methods: In vitro antifungal activity of all the extracts was done by agar slant double dilution method. Candida albicans and Aspergillus fumigatus clinically important strains were used for the study. ketoconazole was used as standards for antifungal assay. Antifungal activity was determinated by evaluating of antifungal parameters values which are MCF (minimal concentration fungicide) and IC50 (Concentration for 50% of inhibition) around each assay. Results: Result showed that the antifungal activity was more pronounced against Aspergillus fumigatus than Candida albicans. The hydroalcoholic extract showed best antifungal activity than ketoconazole. Conclusion: Demonstration of antifungal activity of T. ivorensis provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antifungal substances that could serve as selective agents for infectious disease chemotherapy.
Thérapie, 2010
Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes en forte... more Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes en forte recrudescence chez les malades du SIDA, notre équipe a testé neuf (09) extraits d'une rubiaceae codifiée MISCA sur la croissance in vitro d'une souche de Candida albicans. Les tests antifongiques ont été réalisés sur milieu Sabouraud selon la méthode de la double dilution en tubes penchés. La souche testée est sensible aux neuf extraits de MISCA et l'extrait X 11 obtenu par partition de l'extrait hydro-alcoolique dans un mélange de solvants hexane-eau (v/v) est le plus actif (CMF =3,125 mg/ml). Le tri phytochimique de tous les extraits de MISCA a révélé une forte présence des stérols et des triterpènes dans l'extrait X 11 , l'activité anticandidosique de cette plante serait donc due à ces composés. Ainsi, l'extraction à partir d'un mélange alcool-eau 70 :30 (v/v) suivi d'une partition dans la combinaison hexane-eau 50 :50 (v/v) est celle qui permet de mieux concentrer les principes actifs antifongiques présents dans MISCA.
… des Sciences de Liège, 2009
Bulletin de la Société Royale des Sciences de Liège --- Volume 78 - Année 2009 : 12 - 23 --- Vers... more Bulletin de la Société Royale des Sciences de Liège --- Volume 78 - Année 2009 : 12 - 23 --- Version imprimable. ... Bulletin de la Société Royale des Sciences de Liège, Volume 78 - Année 2009 : 12 - 23 http://popups.ulg.ac.be/SRSL/document.php?id=1167. ...
Journal of Natural Products and Resources, 2020
This study was conducted to make a contribution in the treatment of dermatosis, particularly moth... more This study was conducted to make a contribution in the treatment of dermatosis, particularly moths. Ointments of different concentrations made from the hydroethanolic extract of Terminalia ivorensis and shea butter were used. The best results were obtained with the following concentrations: C4: 0.01 g/mL; C5: 0.0125 g/mL; C6: 0.015 g/mL and C10: 0.025 g/mL for trichophytic clipping moth on eleven (11) selected persons with ringworm and C9: 0.0225 g/mL for microsporic clipping moth on one (1) person. The present study really provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antifungal substances that could serve as selective agents for infectious disease chemotherapy.
Evaluation de l'activité antifongique des extraits totaux de Hugonia platysepala sur les pathogèn... more Evaluation de l'activité antifongique des extraits totaux de Hugonia platysepala sur les pathogènes responsables de cryptococcoses et de candidoses chez les sujets infectés par le VIH Coulibaly et al., J. Appl. Biosci. 2020 Evaluation de l'activité antifongique des extraits totaux de Hugonia platysepala sur les pathogènes responsables de cryptococcoses et de candidoses chez les sujets infectés par le VIH 15047 Evaluation of antifungal activity of Hugonia platysepala extracts on ccryptococcosis and candidiasisresponsible pathogens in HIV-infected people ABSTRACT Objective: To evaluate in vitro the antifungal activity of total extracts from the leaves of Hugonia platysepala, a medicinal plant in southwestern Côte d'Ivoire in order to treat the fungi of people in poor countries infected with HIV/AIDS at a lower cost. Methodology and results: The liquid dilution macromethod and solid-media scattering method have been used for antifungal testing. The results revealed that both water extracts (ETA) and hydro alcoholic (ETOH) have fungicide activity on Cryptococcus neoformans and Candida albicans but ETOH extract is 32 times more active than ETA extract with a 1.56 mg/ml CMI and CMF 50 mg/ml. Tested on an isolate of Candida albicans resistant to Amphotéricin B and Fluconazole, ETOH obtained an inhibition zone above the 10 mm sensitivity threshold. Conclusion and application: ETOH therefore has a fungicide effect on strains of Cryptococcus neoformans and Candida albicans, sensitive or resistant to conventional antifungals regularly prescribed to patients. The phytochemical screening of this ETOH extract revealed the presence of terpenes and polyphenols that could justify its antifungal pharmacological properties. The ETOH extract of Hugonia platysepala, after purification of its active molecules, can be used as an Improved Traditional Drug in the treatment of fungus in HIV-infected people.
Parmi les maladies opportunistes, les aspergilloses, mycoses de l’appareil respiratoire essentiel... more Parmi les maladies opportunistes, les aspergilloses, mycoses de l’appareil respiratoire essentiellement de l’homme et des animaux, sont causees par des champignons du genre Aspergillus [1]. Pour apporter notre contribution a l’eradication de ces mycoses nous avons teste l’efficacite des residus de Terminalia ivorensis A.Chev. (une combretacee) sur la croissance in vitro de Aspergillus fumigatus. Les residus ont ete incorpores a la gelose sabouraud selon la methode de la double dilution en tubes penches. Nous avons ensemence 1000 cellules de Aspergillus fumigatus sur ces milieux. Les cultures ont ete incubees a 30°C pendant 48 heures. Les resultats des tests antifongiques montrent que parmi les dix residus testes, le residu non hexanosoluble X42 (CMF = 40 microg/ml ; CI50 = 4,56 microg/ml) est le plus actif. Par contre le residu aqueux Xaq (CMF = 190 microg/ml ; CI50 = 39,60 microg/ml) est le moins actif. L’utilisation d’un solvant comportant 70% d’ethanol et 30% d’eau suivie ...
The Journal of Phytopharmacology, 2016
Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regula... more Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regularly evaluated in different Laboratories. And, the processes of evaluation of antifungal susceptibility are often different to one laboratory to another. This study was undertaken in the goal to compare parameters obtained (MIC and MFC) with two antifungal susceptibility testing. The first one is Agar slant double dilution tubes method (M1) and the second is disc diffusion method /broth dilution according document CLSI M38 -A2 supplemented to 2% glucose (M2). Ethanol (EtOH 70%), Methanol (MeOH) and n-Hexane (n-HexTG) extracts of leaves of Tectona grandis have been tested in comparison to Itraconazole against two clinicals strains of Aspergillus fumigatus 896/AB and Aspergillus flavus 1006/OM isolated to HIV patients. Results showed that on A. flavus, agar slant method (M1) gave MIC ranging between 200 µg/mL and 800 µg/mL while disc diffusion meth/broth dilution (M2) showed growth inhibiti...
European Scientific Journal, ESJ, 2018
Introduction: Ocimum gratissimum is used in traditional Ivorian medicine for its multiple therape... more Introduction: Ocimum gratissimum is used in traditional Ivorian medicine for its multiple therapeutic virtues. Scope: Evaluate the biological properties of the essential oil of this plant. Methods: Antitumor activity was assessed using the MTS colorimetric assay on six (06) cell lines, the antifungal activity was evaluated by double dilution slant method on four (04) strains of clinical fungi, the potential antioxidant was determined by the DPPH test. Results: The strongest cytotoxic activity was obtained on Jurkat (T lymphoma) with an IC50 = 80 μg / ml. The best antifungal activity was observed on C. albicans with MIC and FMC values obtained at 12.5 μg / ml. Antioxidant activity was evaluated at F = 187 ± 1.57 mM Trolox / ml or (I = 38 ± 0.74%). Conclusion: The essential oil has interesting biological properties like as cytotoxic, antifungal and antioxidant
Journal of Drug Delivery and Therapeutics, 2013
Journal of Intercultural Ethnopharmacology, 2013
Aim: The present study was undertaken to evaluate in vitro antifungal activity of aqueous and hyd... more Aim: The present study was undertaken to evaluate in vitro antifungal activity of aqueous and hydroacoholic extracts from bark of Terminalia ivorensis A. Chev. (Combretaceae). Methods: In vitro antifungal activity of all the extracts was done by agar slant double dilution method. Candida albicans and Aspergillus fumigatus clinically important strains were used for the study. ketoconazole was used as standards for antifungal assay. Antifungal activity was determinated by evaluating of antifungal parameters values which are MCF (minimal concentration fungicide) and IC50 (Concentration for 50% of inhibition) around each assay. Results: Result showed that the antifungal activity was more pronounced against Aspergillus fumigatus than Candida albicans. The hydroalcoholic extract showed best antifungal activity than ketoconazole. Conclusion: Demonstration of antifungal activity of T. ivorensis provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antifungal substances that could serve as selective agents for infectious disease chemotherapy.
Thérapie, 2010
Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes en forte... more Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes en forte recrudescence chez les malades du SIDA, notre équipe a testé neuf (09) extraits d'une rubiaceae codifiée MISCA sur la croissance in vitro d'une souche de Candida albicans. Les tests antifongiques ont été réalisés sur milieu Sabouraud selon la méthode de la double dilution en tubes penchés. La souche testée est sensible aux neuf extraits de MISCA et l'extrait X 11 obtenu par partition de l'extrait hydro-alcoolique dans un mélange de solvants hexane-eau (v/v) est le plus actif (CMF =3,125 mg/ml). Le tri phytochimique de tous les extraits de MISCA a révélé une forte présence des stérols et des triterpènes dans l'extrait X 11 , l'activité anticandidosique de cette plante serait donc due à ces composés. Ainsi, l'extraction à partir d'un mélange alcool-eau 70 :30 (v/v) suivi d'une partition dans la combinaison hexane-eau 50 :50 (v/v) est celle qui permet de mieux concentrer les principes actifs antifongiques présents dans MISCA.
… des Sciences de Liège, 2009
Bulletin de la Société Royale des Sciences de Liège --- Volume 78 - Année 2009 : 12 - 23 --- Vers... more Bulletin de la Société Royale des Sciences de Liège --- Volume 78 - Année 2009 : 12 - 23 --- Version imprimable. ... Bulletin de la Société Royale des Sciences de Liège, Volume 78 - Année 2009 : 12 - 23 http://popups.ulg.ac.be/SRSL/document.php?id=1167. ...
Journal of Natural Products and Resources, 2020
This study was conducted to make a contribution in the treatment of dermatosis, particularly moth... more This study was conducted to make a contribution in the treatment of dermatosis, particularly moths. Ointments of different concentrations made from the hydroethanolic extract of Terminalia ivorensis and shea butter were used. The best results were obtained with the following concentrations: C4: 0.01 g/mL; C5: 0.0125 g/mL; C6: 0.015 g/mL and C10: 0.025 g/mL for trichophytic clipping moth on eleven (11) selected persons with ringworm and C9: 0.0225 g/mL for microsporic clipping moth on one (1) person. The present study really provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antifungal substances that could serve as selective agents for infectious disease chemotherapy.
Evaluation de l'activité antifongique des extraits totaux de Hugonia platysepala sur les pathogèn... more Evaluation de l'activité antifongique des extraits totaux de Hugonia platysepala sur les pathogènes responsables de cryptococcoses et de candidoses chez les sujets infectés par le VIH Coulibaly et al., J. Appl. Biosci. 2020 Evaluation de l'activité antifongique des extraits totaux de Hugonia platysepala sur les pathogènes responsables de cryptococcoses et de candidoses chez les sujets infectés par le VIH 15047 Evaluation of antifungal activity of Hugonia platysepala extracts on ccryptococcosis and candidiasisresponsible pathogens in HIV-infected people ABSTRACT Objective: To evaluate in vitro the antifungal activity of total extracts from the leaves of Hugonia platysepala, a medicinal plant in southwestern Côte d'Ivoire in order to treat the fungi of people in poor countries infected with HIV/AIDS at a lower cost. Methodology and results: The liquid dilution macromethod and solid-media scattering method have been used for antifungal testing. The results revealed that both water extracts (ETA) and hydro alcoholic (ETOH) have fungicide activity on Cryptococcus neoformans and Candida albicans but ETOH extract is 32 times more active than ETA extract with a 1.56 mg/ml CMI and CMF 50 mg/ml. Tested on an isolate of Candida albicans resistant to Amphotéricin B and Fluconazole, ETOH obtained an inhibition zone above the 10 mm sensitivity threshold. Conclusion and application: ETOH therefore has a fungicide effect on strains of Cryptococcus neoformans and Candida albicans, sensitive or resistant to conventional antifungals regularly prescribed to patients. The phytochemical screening of this ETOH extract revealed the presence of terpenes and polyphenols that could justify its antifungal pharmacological properties. The ETOH extract of Hugonia platysepala, after purification of its active molecules, can be used as an Improved Traditional Drug in the treatment of fungus in HIV-infected people.