Sitesh Bachar - Academia.edu (original) (raw)

Papers by Sitesh Bachar

Research paper thumbnail of A Review on Mechanistic Insight of Plant Derived Anticancer Bioactive Phytocompounds and Their Structure Activity Relationship

Molecules

Cancer is a disorder that rigorously affects the human population worldwide. There is a steady de... more Cancer is a disorder that rigorously affects the human population worldwide. There is a steady demand for new remedies to both treat and prevent this life-threatening sickness due to toxicities, drug resistance and therapeutic failures in current conventional therapies. Researchers around the world are drawing their attention towards compounds of natural origin. For decades, human beings have been using the flora of the world as a source of cancer chemotherapeutic agents. Currently, clinically approved anticancer compounds are vincristine, vinblastine, taxanes, and podophyllotoxin, all of which come from natural sources. With the triumph of these compounds that have been developed into staple drug products for most cancer therapies, new technologies are now appearing to search for novel biomolecules with anticancer activities. Ellipticine, camptothecin, combretastatin, curcumin, homoharringtonine and others are plant derived bioactive phytocompounds with potential anticancer propert...

Research paper thumbnail of A Review of Medicinal Plants with Antiviral Activity Available in Bangladesh and Mechanistic Insight Into Their Bioactive Metabolites on SARS-CoV-2, HIV and HBV

Frontiers in Pharmacology, 2021

Currently, viral infection is the most serious health issue which causing unexpected higher rate ... more Currently, viral infection is the most serious health issue which causing unexpected higher rate of death globally. Many viruses are not yet curable, such as corona virus-2 (SARS-CoV-2), human immunodeficiency virus (HIV), hepatitis virus, human papilloma virus and so others. Furthermore, the toxicities and ineffective responses to resistant strains of synthetic antiviral drugs have reinforced the search of effective and alternative treatment options, such as plant-derived antiviral drug molecules. Therefore, in the present review, an attempt has been taken to summarize the medicinal plants reported for exhibiting antiviral activities available in Bangladesh along with discussing the mechanistic insights into their bioactive components against three most hazardous viruses, namely SARS-CoV-2, HIV, and HBV. The review covers 46 medicinal plants with antiviral activity from 25 families. Among the reported 79 bioactive compounds having antiviral activities isolated from these plants, ab...

Research paper thumbnail of Synthesis and structure-activity-relationships of indan acid derivatives as analgesic and anti-inflammatory agents

Review Journal of Chemistry, 2016

Indan ring system, also known as indane or benzocyclopentane, is an important chemical feature as... more Indan ring system, also known as indane or benzocyclopentane, is an important chemical feature associated with various biological activities. Among the drugs possessing an indan ring system, 1H-indene-3-acetic acid-5-fluoro-2-methyl-1-[4-(methylsulfinyl)phenyl]methylene (Sulindac) and 6-chloro-5-cyclohexyl indan-1-carboxylic acid (Clidanac) are well known anti-inflammatory agents, whilst indan-1,3-dione is an anticoagulant. Over the years, several indan derivatives, particularly indan acid derivatives, have been synthesized and their potential analgesic and anti-inflammatory properties have been studied. This review critically appraises various synthetic protocols and structure-activity-relationships of indan acids as analgesic and anti-inflammatory agents with low ulcerogenicity.

Research paper thumbnail of Analgesic and Anti-inflammatory Activities of Imperata cylindrica

Dhaka University Journal of Pharmaceutical Sciences, Jun 15, 2007

Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dha... more Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

Research paper thumbnail of Evaluation of Justicia adhatoda L. syn. Adhatoda vasica Nees extract by major metabolite analysis

Indian journal of traditional knowledge

The present study reveals an improved method for the evaluation of Adhatoda vasica extract by qua... more The present study reveals an improved method for the evaluation of Adhatoda vasica extract by quantitation of major metabolite vasicine. The plant powder was extracted with methanol and evaporated in vaccuo to obtain an oily residue. The residue was diluted with aqueous solution of citric acid (1%) and was further extracted with chloroform. The preliminary screening for alkaloid was done with alkaloidal reagents and the presence of vasicine was assessed by thin layer chromatography (TLC). The extractive was then subjected to column chromatography using a mixture of ethyl acetate and methanol adjusted to pH 9.5 with liquid ammonia as mobile phase. The TLC chromatograms of the collected column fractions were developed and the fractions indicated the presences of vasicine were combined together. The residue obtained from evaporation of solvent was subjected to preparative TLC along with reference standard to get pure crystalline vasicine. The compound vasicine was ascertained by UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of vasicine was carried out by reversed phase HPLC method. About 99.50% of pure vasicine (647 mg) compared to reference standard was obtained from 250 gm of leaf powder (0.2588% w/w).

Research paper thumbnail of Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

Dhaka University Journal of Pharmaceutical Sciences, 2015

Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as an... more Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 ?g/mL in the solution of amorphous residue (50 ?g/mL). After quantitative determinatio...

Research paper thumbnail of Comparative pharmacokinetic analysis with two omeprazole formulations “Proceptin®” and “Losec®” in healthy subjects

Archives of Medical and Biomedical Research, 2015

The pharmacokinetics of omeprazole pharmaceutical products Proceptin® 20mg capsule and Losec® 20m... more The pharmacokinetics of omeprazole pharmaceutical products Proceptin® 20mg capsule and Losec® 20mg MUPS tablet were compared in healthy subjects. The study was an open-label, randomized, two-treatment, two-sequence, two-way crossover, single-dose bioavailability study conducted under fasting conditions with a wash out period of seven days between the two administrations. Blood samples were collected pre-dosing and at 0.5-24.0 h post administration of a single oral dose of either of the formulation followed by HPLC analysis. Twenty-eight healthy male subjects (20-28 years) participated in the study. Only four subjects dropped from the study and the other 24 completed the study and were included in the pharmacokinetic and statistical analysis. Evaluated mean (±SD) values of prime pharmacokinetic parameters for reference and test products were C max-345.28 (±42.38) and 316.23 (±26.12) ng/mL, t max-2.28 (±0.16) and 2.69 (±0.23) h, AUC 0-24-710.01 (±92.51) and 771.13 (±102.35) h-ng/ml and AUC 0-∞-848.21 (±65.31) and 902.56 (±45.23) h.ng/mL, respectively with no significant (p>0.05) differences in paired t-test. Moreover, 90% CI for the AUC 0-24 , and C max values were 89.245-103.154% and 81.634-102.211% respectively were within the predetermined FDA bioequivalence range of 80-125%. On the basis of the pharmacokinetic parameters AUC 0-24 , the relative bioavailability of the test preparation Proceptin 20 capsule was 108.61% of that of the reference preparation Losec 20mg MUPS tablet. This study stipulated that, the test and reference formulations of omeprazole meet the regulatory criteria for bioequivalence. Thus the test product Proceptin ® 20mg may be supplanted for reference product Losec ® 20mg MUPS tablet in oral administration.

Research paper thumbnail of Anti hyperlipidemic Activity of Flowers of Punica granatum in Poloxamer-407 Induced Hyperlipidemic Mice Model

Pharmacognosy Journal, 2012

Pomegranate is an important medicinal plant containing versatile bioactive compounds and traditio... more Pomegranate is an important medicinal plant containing versatile bioactive compounds and traditionally was used in the treatment of different diseases. The ripe fruit is tonic, astringent, laxative and diuretic, used in brain diseases, chest troubles, bronchitis and earache. Bark and fruit rind are administered orally to prevent dysentery, diarrhea, piles, bronchitis, biliousness and as an anthelmintic. [2] The dried flowers, known as Gulnar, are efficaceous to treat hematuria, hemoptysis, diarrhea, dysentery, bronchitis, nasal hemorrhage. [3] Pomegranate flowers have been prescribed in Unani and Ayurvedic medicines for the treatment of diabetes. [4] It has been demonstrated that flower extract shows hypoglycemic activity in normal and alloxan-induced diabetic animals. [5] Flower juice is recommended as a gargle for sore throat, oral and throat inflammation, in leucorrhea, hemorrhage and ulcers of the uterus and rectus. [3] Some studies have reported that both the pomegranate flowers and fruit extracts exhibited high activity on lowering

Research paper thumbnail of The Analgesic and Anti-Inflammatory Activities of the Extracts of Phyllanthus reticulatus. in Mice Model

Research paper thumbnail of Antidiarrheal Activity of the Methanol Extract of Ludwigia Hyssopifolia Linn

… J Pharm Sci, 2003

Dried whole plant parts of Ludwigia hyssopifolia were subjected to successive cold extraction wit... more Dried whole plant parts of Ludwigia hyssopifolia were subjected to successive cold extraction with n-hexane, ethylacetate and methanol. The methanol extract (LHM), obtained as 1% yield, showed significant antidiarrheal property by reducing diarrheal episodes in castor ...

Research paper thumbnail of Cannabinoid as Potential Aromatase Inhibitor Through Molecular Modeling and Screening for Anti-Cancer Activity

Dhaka University Journal of Pharmaceutical Sciences

Inhibition of aromatase (CYTP450), a key enzyme in the estrogen biosynthesis, could result in reg... more Inhibition of aromatase (CYTP450), a key enzyme in the estrogen biosynthesis, could result in regression of estrogen-dependent tumors and even prevent the promotion of breast cancer. The present research has been designed for searching a potent chemical moiety from natural sources to inhibit aromatase enzyme, the overfunctionality of which causes the breast cancer. Cannabis sativa contains a very much promising group of cannabinoids with more than 66 compounds with reported anticancer property and for the search of a target specific potent aromatase inhibitor, 61 cannabinoids from C. sativa were selected. The Structures Data File (SDF) of these ligand molecules were subjected to docking studies at the binding site of aromatase X-ray crystallographic structure based on lower resolution of the protein crystal structure and higher docking accuracy, predicted by calculating the correlation between experimental activities and Glide dock scores and compared with the standard aromatase lig...

Research paper thumbnail of Change in Antibiotic Susceptibility Pattern of Clinical Bacterial Isolates from Two Hospitals in Dhaka, Bangladesh over a Period of Three Years

Microbiology Research Journal International

Introduction: It is established that improper use of antibiotics leads to rapid development of ba... more Introduction: It is established that improper use of antibiotics leads to rapid development of bacterial antibiotic resistance. We investigated changes in the antibiotic susceptibility pattern of pathogenic bacteria in a megacity where improper antibiotic use is common. Methodology: Data on the antibiotic susceptibility pattern of clinical isolates to 28 commonly used antibiotics was obtained from two hospitals at time point A (Jan-Dec 2009, or Nov-Dec 2010) and at about 12-36 months later (time point B), and the data were compared using the one-sided test for equality of proportions. For large samples, tests using Z-score and normal distribution were conducted; for small samples, Fisher's exact test was performed.

Research paper thumbnail of Clinical Interventions of Critical Care Pharmacist in the Therapeutic Management of Critically Ill Patients: a Retrospective Study in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences

Critically ill patients at ICU are treated with poly pharmacy and conservative drug management is... more Critically ill patients at ICU are treated with poly pharmacy and conservative drug management is necessary for ensuring drugs’ safety and accuracy. The objective of this study was to analyze the qualitative intervention of critical care pharmacists (CCP) in critically ill patients’ effective medication management. This was a 6 months long observational study. All the provided suggestions of CCP were categorized into- A (drug-drug interaction), B (addition of new drug therapies), C (rational dosing of antibiotics), D (acceleration or deceleration of the doses) and E (adverse drug reaction). Out of total CCP’s 650 suggestions, 566 (87.08%) suggestions were accepted by doctors and modified the therapies, accordingly. CCP being a part of ICU’s multi professional team contribute the professional roles in generating safe, appropriate and quality prescriptions, which finally turns into quality pharmacotherapy for critically ill patients at ICU. Dhaka Univ. J. Pharm. Sci. 18(1): 113-119, 2...

Research paper thumbnail of Early Event Detection and Discontinuation of the Culprit Drug Increases the Rate of Survival in Severe Cutaneous Adverse Drug Reactions: A Multi-Center Study in Bangladesh

International Journal of Sciences

Adverse drug reactions are completely unwanted drug reactions derived from self-medications and a... more Adverse drug reactions are completely unwanted drug reactions derived from self-medications and among those unpleasant reactions, severe cutaneous adverse drug reactions like, Stevens-Johnson Syndrome, Toxic Epidermal Necrolysis, Acute Generalized Exanthematous Pustolosis and Drug Reaction with Eosinophilia and Systemic Symptoms, are mostly life threatening harms, globally. The main objective of this study was to find out the impact of early detection of the adverse drug events and consequently prompt withdrawn of the suspected culprit drugs in the ultimate survival rate among the patients experienced with these types of unwelcome life threatening events in Bangladesh. Here, we found that antibiotics were accounted for most adverse drug reactions (81.13%, n=53) including serious reactions. This study also showed that if the adverse drug event is possibly detected with in the first 48 hours then, 72.73% (n=11) patients were survived from severe cutaneous adverse drug reactions, where as if delayed than 48 hours, only 20% (n=5) patients were survived. Similarly, 77.77% (n=9) patients were survived after the proper treatment when suspected culprit drugs were discontinued within first 48 hours and late discontinuation resulted in only 28.57% (n=7) survival rate among these patients. So, prompt response through early event-detection and discontinuation of the culprit drug, to a serious cutaneous adverse drug reaction can tremendously save life by providing early necessary management and required treatment.

Research paper thumbnail of Phytochemical investigations and antioxidant potential of roots of Leea macrophylla (Roxb.)

BMC research notes, Jan 6, 2017

Oleanolic acid (NZ-15), 7 α, 28-olean diol (NZ-38) and Stigmasterol (NZ-14) were isolated from th... more Oleanolic acid (NZ-15), 7 α, 28-olean diol (NZ-38) and Stigmasterol (NZ-14) were isolated from the ethanolic extracts of the roots of Leea macrophylla (Family: Leeaceae) by using chromatographic analysis. This is the first report of isolation of these compounds from this plant. Their structures were constructed by spectroscopic analysis and by comparing the data with the published one. Subsequently the ethanolic extract was fractionated with two organic solvents and all the fractions were studied to evaluate their in vitro antioxidant property. The ethanolic extract was fractionated with two organic solvents and all the fractions were studied to evaluate their in vitro antioxidant property by DPPH free radical scavenging assay, superoxide anion radical scavenging assay, nitric oxide radical scavenging assay, and reducing power assay. In the DPPH free radical scavenging assay and superoxide radical scavenging assay, the ethyl acetate soluble fraction of ethanolic extract revealed the...

Research paper thumbnail of Standardization of Adhatoda vasica Nees Market Preparations by RP-HPLC Method

Dhaka University Journal of Pharmaceutical Sciences, 2016

In recent times, quality control of herbal and traditional medicines with modern scientific techn... more In recent times, quality control of herbal and traditional medicines with modern scientific techniques and knowledge are of great concern. The present study reveals a simple and improved reversed phase HPLC method for qualitative analyses of Adhatoda vasica Nees market preparations via quantitation of its major metabolite, vasicine as a marker compound. Three market preparations, each of four different herbal and traditional manufacturers, were analyzed. The market preparations were extracted with chloroform and the residue obtained from extraction of each market preparation was analyzed for quantitation of vasicine by RP-HPLC method with ODS column using a mixture of water and methanol (60:40) as mobile phase at a flow rate of 0.5 ml/min. The estimated quantities of vasicine compared to reference standard for marketed products of four different manufacturers were found to be 1.502 ± 0.064 g, 1.590 ± 0.081 g, 1.761 ± 0.061 g and 1.627 ± 0.082 g, respectively per 100 ml of preparatio...

Research paper thumbnail of Antitumor and Antibacterial Activity of Ethylacetate Extract of Ludwigia Hyssopifolia Linn and Its Active Principle Piperine

An alkaloid constituent 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine, trivial nam... more An alkaloid constituent 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine, trivial name piperine was isolated from Ludwigia hyssopifolia Linn. (Family-Onagraceae). The ethylacetate extract of the plant and the isolated compound piperine were studied for antitumor and in vitro antibacterial activity. Ethylacetate extract showed 73.05 and 84.14% inhibition of Agrobacterium tumefaciens-induced crown gall tumor formation in potato disc. Piperine exhibited antitumor activity with IC 50 value of 13.50 µg/disc. Both ethylacetate extract and piperine showed mild to moderate antibacterial activity against selected Gram-positive and Gram-negative bacteria.

Research paper thumbnail of Shrotriya et al PJPS

Research paper thumbnail of Analgesic and Anti-inflammatory Activities of Desmodium triflorum DC

Journal of Biological Sciences, May 1, 2005

Research paper thumbnail of Development and evaluation of Water dispersible tablets of Acyclovir

The present investigation concerns the development of water dispersible tablets of Acyclovir whic... more The present investigation concerns the development of water dispersible tablets of Acyclovir which were designed to enhance the onset of action of Acyclovir. The acyclovir water dispersible tablet was prepared by using Veegum F (Magnesium Aluminum Silicate) and Sodium Starch Glycolate as superdisintegrating agent, Avicel PH 102 as a diluent, Povidone K30 as a binder and carried out studies for weight variation, thickness, hardness, content uniformity, disintegrating time, dispersion time, wetting time and in vitro drug release. Tablets were prepared by using wet granulation method. Out of five, formulation F2 exhibited highest drug release 85.11% and formulation F4 showed drug release 85.42% in 10 min. They also exhibited the shortest wetting time 57.56 and 54.46 second with dispersion time 78 and 110 second respectively. It was concluded that water dispersible tablets with enhanced dissolution rate can be made using selective superdisintegrants.

Research paper thumbnail of A Review on Mechanistic Insight of Plant Derived Anticancer Bioactive Phytocompounds and Their Structure Activity Relationship

Molecules

Cancer is a disorder that rigorously affects the human population worldwide. There is a steady de... more Cancer is a disorder that rigorously affects the human population worldwide. There is a steady demand for new remedies to both treat and prevent this life-threatening sickness due to toxicities, drug resistance and therapeutic failures in current conventional therapies. Researchers around the world are drawing their attention towards compounds of natural origin. For decades, human beings have been using the flora of the world as a source of cancer chemotherapeutic agents. Currently, clinically approved anticancer compounds are vincristine, vinblastine, taxanes, and podophyllotoxin, all of which come from natural sources. With the triumph of these compounds that have been developed into staple drug products for most cancer therapies, new technologies are now appearing to search for novel biomolecules with anticancer activities. Ellipticine, camptothecin, combretastatin, curcumin, homoharringtonine and others are plant derived bioactive phytocompounds with potential anticancer propert...

Research paper thumbnail of A Review of Medicinal Plants with Antiviral Activity Available in Bangladesh and Mechanistic Insight Into Their Bioactive Metabolites on SARS-CoV-2, HIV and HBV

Frontiers in Pharmacology, 2021

Currently, viral infection is the most serious health issue which causing unexpected higher rate ... more Currently, viral infection is the most serious health issue which causing unexpected higher rate of death globally. Many viruses are not yet curable, such as corona virus-2 (SARS-CoV-2), human immunodeficiency virus (HIV), hepatitis virus, human papilloma virus and so others. Furthermore, the toxicities and ineffective responses to resistant strains of synthetic antiviral drugs have reinforced the search of effective and alternative treatment options, such as plant-derived antiviral drug molecules. Therefore, in the present review, an attempt has been taken to summarize the medicinal plants reported for exhibiting antiviral activities available in Bangladesh along with discussing the mechanistic insights into their bioactive components against three most hazardous viruses, namely SARS-CoV-2, HIV, and HBV. The review covers 46 medicinal plants with antiviral activity from 25 families. Among the reported 79 bioactive compounds having antiviral activities isolated from these plants, ab...

Research paper thumbnail of Synthesis and structure-activity-relationships of indan acid derivatives as analgesic and anti-inflammatory agents

Review Journal of Chemistry, 2016

Indan ring system, also known as indane or benzocyclopentane, is an important chemical feature as... more Indan ring system, also known as indane or benzocyclopentane, is an important chemical feature associated with various biological activities. Among the drugs possessing an indan ring system, 1H-indene-3-acetic acid-5-fluoro-2-methyl-1-[4-(methylsulfinyl)phenyl]methylene (Sulindac) and 6-chloro-5-cyclohexyl indan-1-carboxylic acid (Clidanac) are well known anti-inflammatory agents, whilst indan-1,3-dione is an anticoagulant. Over the years, several indan derivatives, particularly indan acid derivatives, have been synthesized and their potential analgesic and anti-inflammatory properties have been studied. This review critically appraises various synthetic protocols and structure-activity-relationships of indan acids as analgesic and anti-inflammatory agents with low ulcerogenicity.

Research paper thumbnail of Analgesic and Anti-inflammatory Activities of Imperata cylindrica

Dhaka University Journal of Pharmaceutical Sciences, Jun 15, 2007

Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dha... more Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

Research paper thumbnail of Evaluation of Justicia adhatoda L. syn. Adhatoda vasica Nees extract by major metabolite analysis

Indian journal of traditional knowledge

The present study reveals an improved method for the evaluation of Adhatoda vasica extract by qua... more The present study reveals an improved method for the evaluation of Adhatoda vasica extract by quantitation of major metabolite vasicine. The plant powder was extracted with methanol and evaporated in vaccuo to obtain an oily residue. The residue was diluted with aqueous solution of citric acid (1%) and was further extracted with chloroform. The preliminary screening for alkaloid was done with alkaloidal reagents and the presence of vasicine was assessed by thin layer chromatography (TLC). The extractive was then subjected to column chromatography using a mixture of ethyl acetate and methanol adjusted to pH 9.5 with liquid ammonia as mobile phase. The TLC chromatograms of the collected column fractions were developed and the fractions indicated the presences of vasicine were combined together. The residue obtained from evaporation of solvent was subjected to preparative TLC along with reference standard to get pure crystalline vasicine. The compound vasicine was ascertained by UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of vasicine was carried out by reversed phase HPLC method. About 99.50% of pure vasicine (647 mg) compared to reference standard was obtained from 250 gm of leaf powder (0.2588% w/w).

Research paper thumbnail of Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

Dhaka University Journal of Pharmaceutical Sciences, 2015

Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as an... more Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 ?g/mL in the solution of amorphous residue (50 ?g/mL). After quantitative determinatio...

Research paper thumbnail of Comparative pharmacokinetic analysis with two omeprazole formulations “Proceptin®” and “Losec®” in healthy subjects

Archives of Medical and Biomedical Research, 2015

The pharmacokinetics of omeprazole pharmaceutical products Proceptin® 20mg capsule and Losec® 20m... more The pharmacokinetics of omeprazole pharmaceutical products Proceptin® 20mg capsule and Losec® 20mg MUPS tablet were compared in healthy subjects. The study was an open-label, randomized, two-treatment, two-sequence, two-way crossover, single-dose bioavailability study conducted under fasting conditions with a wash out period of seven days between the two administrations. Blood samples were collected pre-dosing and at 0.5-24.0 h post administration of a single oral dose of either of the formulation followed by HPLC analysis. Twenty-eight healthy male subjects (20-28 years) participated in the study. Only four subjects dropped from the study and the other 24 completed the study and were included in the pharmacokinetic and statistical analysis. Evaluated mean (±SD) values of prime pharmacokinetic parameters for reference and test products were C max-345.28 (±42.38) and 316.23 (±26.12) ng/mL, t max-2.28 (±0.16) and 2.69 (±0.23) h, AUC 0-24-710.01 (±92.51) and 771.13 (±102.35) h-ng/ml and AUC 0-∞-848.21 (±65.31) and 902.56 (±45.23) h.ng/mL, respectively with no significant (p>0.05) differences in paired t-test. Moreover, 90% CI for the AUC 0-24 , and C max values were 89.245-103.154% and 81.634-102.211% respectively were within the predetermined FDA bioequivalence range of 80-125%. On the basis of the pharmacokinetic parameters AUC 0-24 , the relative bioavailability of the test preparation Proceptin 20 capsule was 108.61% of that of the reference preparation Losec 20mg MUPS tablet. This study stipulated that, the test and reference formulations of omeprazole meet the regulatory criteria for bioequivalence. Thus the test product Proceptin ® 20mg may be supplanted for reference product Losec ® 20mg MUPS tablet in oral administration.

Research paper thumbnail of Anti hyperlipidemic Activity of Flowers of Punica granatum in Poloxamer-407 Induced Hyperlipidemic Mice Model

Pharmacognosy Journal, 2012

Pomegranate is an important medicinal plant containing versatile bioactive compounds and traditio... more Pomegranate is an important medicinal plant containing versatile bioactive compounds and traditionally was used in the treatment of different diseases. The ripe fruit is tonic, astringent, laxative and diuretic, used in brain diseases, chest troubles, bronchitis and earache. Bark and fruit rind are administered orally to prevent dysentery, diarrhea, piles, bronchitis, biliousness and as an anthelmintic. [2] The dried flowers, known as Gulnar, are efficaceous to treat hematuria, hemoptysis, diarrhea, dysentery, bronchitis, nasal hemorrhage. [3] Pomegranate flowers have been prescribed in Unani and Ayurvedic medicines for the treatment of diabetes. [4] It has been demonstrated that flower extract shows hypoglycemic activity in normal and alloxan-induced diabetic animals. [5] Flower juice is recommended as a gargle for sore throat, oral and throat inflammation, in leucorrhea, hemorrhage and ulcers of the uterus and rectus. [3] Some studies have reported that both the pomegranate flowers and fruit extracts exhibited high activity on lowering

Research paper thumbnail of The Analgesic and Anti-Inflammatory Activities of the Extracts of Phyllanthus reticulatus. in Mice Model

Research paper thumbnail of Antidiarrheal Activity of the Methanol Extract of Ludwigia Hyssopifolia Linn

… J Pharm Sci, 2003

Dried whole plant parts of Ludwigia hyssopifolia were subjected to successive cold extraction wit... more Dried whole plant parts of Ludwigia hyssopifolia were subjected to successive cold extraction with n-hexane, ethylacetate and methanol. The methanol extract (LHM), obtained as 1% yield, showed significant antidiarrheal property by reducing diarrheal episodes in castor ...

Research paper thumbnail of Cannabinoid as Potential Aromatase Inhibitor Through Molecular Modeling and Screening for Anti-Cancer Activity

Dhaka University Journal of Pharmaceutical Sciences

Inhibition of aromatase (CYTP450), a key enzyme in the estrogen biosynthesis, could result in reg... more Inhibition of aromatase (CYTP450), a key enzyme in the estrogen biosynthesis, could result in regression of estrogen-dependent tumors and even prevent the promotion of breast cancer. The present research has been designed for searching a potent chemical moiety from natural sources to inhibit aromatase enzyme, the overfunctionality of which causes the breast cancer. Cannabis sativa contains a very much promising group of cannabinoids with more than 66 compounds with reported anticancer property and for the search of a target specific potent aromatase inhibitor, 61 cannabinoids from C. sativa were selected. The Structures Data File (SDF) of these ligand molecules were subjected to docking studies at the binding site of aromatase X-ray crystallographic structure based on lower resolution of the protein crystal structure and higher docking accuracy, predicted by calculating the correlation between experimental activities and Glide dock scores and compared with the standard aromatase lig...

Research paper thumbnail of Change in Antibiotic Susceptibility Pattern of Clinical Bacterial Isolates from Two Hospitals in Dhaka, Bangladesh over a Period of Three Years

Microbiology Research Journal International

Introduction: It is established that improper use of antibiotics leads to rapid development of ba... more Introduction: It is established that improper use of antibiotics leads to rapid development of bacterial antibiotic resistance. We investigated changes in the antibiotic susceptibility pattern of pathogenic bacteria in a megacity where improper antibiotic use is common. Methodology: Data on the antibiotic susceptibility pattern of clinical isolates to 28 commonly used antibiotics was obtained from two hospitals at time point A (Jan-Dec 2009, or Nov-Dec 2010) and at about 12-36 months later (time point B), and the data were compared using the one-sided test for equality of proportions. For large samples, tests using Z-score and normal distribution were conducted; for small samples, Fisher's exact test was performed.

Research paper thumbnail of Clinical Interventions of Critical Care Pharmacist in the Therapeutic Management of Critically Ill Patients: a Retrospective Study in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences

Critically ill patients at ICU are treated with poly pharmacy and conservative drug management is... more Critically ill patients at ICU are treated with poly pharmacy and conservative drug management is necessary for ensuring drugs’ safety and accuracy. The objective of this study was to analyze the qualitative intervention of critical care pharmacists (CCP) in critically ill patients’ effective medication management. This was a 6 months long observational study. All the provided suggestions of CCP were categorized into- A (drug-drug interaction), B (addition of new drug therapies), C (rational dosing of antibiotics), D (acceleration or deceleration of the doses) and E (adverse drug reaction). Out of total CCP’s 650 suggestions, 566 (87.08%) suggestions were accepted by doctors and modified the therapies, accordingly. CCP being a part of ICU’s multi professional team contribute the professional roles in generating safe, appropriate and quality prescriptions, which finally turns into quality pharmacotherapy for critically ill patients at ICU. Dhaka Univ. J. Pharm. Sci. 18(1): 113-119, 2...

Research paper thumbnail of Early Event Detection and Discontinuation of the Culprit Drug Increases the Rate of Survival in Severe Cutaneous Adverse Drug Reactions: A Multi-Center Study in Bangladesh

International Journal of Sciences

Adverse drug reactions are completely unwanted drug reactions derived from self-medications and a... more Adverse drug reactions are completely unwanted drug reactions derived from self-medications and among those unpleasant reactions, severe cutaneous adverse drug reactions like, Stevens-Johnson Syndrome, Toxic Epidermal Necrolysis, Acute Generalized Exanthematous Pustolosis and Drug Reaction with Eosinophilia and Systemic Symptoms, are mostly life threatening harms, globally. The main objective of this study was to find out the impact of early detection of the adverse drug events and consequently prompt withdrawn of the suspected culprit drugs in the ultimate survival rate among the patients experienced with these types of unwelcome life threatening events in Bangladesh. Here, we found that antibiotics were accounted for most adverse drug reactions (81.13%, n=53) including serious reactions. This study also showed that if the adverse drug event is possibly detected with in the first 48 hours then, 72.73% (n=11) patients were survived from severe cutaneous adverse drug reactions, where as if delayed than 48 hours, only 20% (n=5) patients were survived. Similarly, 77.77% (n=9) patients were survived after the proper treatment when suspected culprit drugs were discontinued within first 48 hours and late discontinuation resulted in only 28.57% (n=7) survival rate among these patients. So, prompt response through early event-detection and discontinuation of the culprit drug, to a serious cutaneous adverse drug reaction can tremendously save life by providing early necessary management and required treatment.

Research paper thumbnail of Phytochemical investigations and antioxidant potential of roots of Leea macrophylla (Roxb.)

BMC research notes, Jan 6, 2017

Oleanolic acid (NZ-15), 7 α, 28-olean diol (NZ-38) and Stigmasterol (NZ-14) were isolated from th... more Oleanolic acid (NZ-15), 7 α, 28-olean diol (NZ-38) and Stigmasterol (NZ-14) were isolated from the ethanolic extracts of the roots of Leea macrophylla (Family: Leeaceae) by using chromatographic analysis. This is the first report of isolation of these compounds from this plant. Their structures were constructed by spectroscopic analysis and by comparing the data with the published one. Subsequently the ethanolic extract was fractionated with two organic solvents and all the fractions were studied to evaluate their in vitro antioxidant property. The ethanolic extract was fractionated with two organic solvents and all the fractions were studied to evaluate their in vitro antioxidant property by DPPH free radical scavenging assay, superoxide anion radical scavenging assay, nitric oxide radical scavenging assay, and reducing power assay. In the DPPH free radical scavenging assay and superoxide radical scavenging assay, the ethyl acetate soluble fraction of ethanolic extract revealed the...

Research paper thumbnail of Standardization of Adhatoda vasica Nees Market Preparations by RP-HPLC Method

Dhaka University Journal of Pharmaceutical Sciences, 2016

In recent times, quality control of herbal and traditional medicines with modern scientific techn... more In recent times, quality control of herbal and traditional medicines with modern scientific techniques and knowledge are of great concern. The present study reveals a simple and improved reversed phase HPLC method for qualitative analyses of Adhatoda vasica Nees market preparations via quantitation of its major metabolite, vasicine as a marker compound. Three market preparations, each of four different herbal and traditional manufacturers, were analyzed. The market preparations were extracted with chloroform and the residue obtained from extraction of each market preparation was analyzed for quantitation of vasicine by RP-HPLC method with ODS column using a mixture of water and methanol (60:40) as mobile phase at a flow rate of 0.5 ml/min. The estimated quantities of vasicine compared to reference standard for marketed products of four different manufacturers were found to be 1.502 ± 0.064 g, 1.590 ± 0.081 g, 1.761 ± 0.061 g and 1.627 ± 0.082 g, respectively per 100 ml of preparatio...

Research paper thumbnail of Antitumor and Antibacterial Activity of Ethylacetate Extract of Ludwigia Hyssopifolia Linn and Its Active Principle Piperine

An alkaloid constituent 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine, trivial nam... more An alkaloid constituent 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine, trivial name piperine was isolated from Ludwigia hyssopifolia Linn. (Family-Onagraceae). The ethylacetate extract of the plant and the isolated compound piperine were studied for antitumor and in vitro antibacterial activity. Ethylacetate extract showed 73.05 and 84.14% inhibition of Agrobacterium tumefaciens-induced crown gall tumor formation in potato disc. Piperine exhibited antitumor activity with IC 50 value of 13.50 µg/disc. Both ethylacetate extract and piperine showed mild to moderate antibacterial activity against selected Gram-positive and Gram-negative bacteria.

Research paper thumbnail of Shrotriya et al PJPS

Research paper thumbnail of Analgesic and Anti-inflammatory Activities of Desmodium triflorum DC

Journal of Biological Sciences, May 1, 2005

Research paper thumbnail of Development and evaluation of Water dispersible tablets of Acyclovir

The present investigation concerns the development of water dispersible tablets of Acyclovir whic... more The present investigation concerns the development of water dispersible tablets of Acyclovir which were designed to enhance the onset of action of Acyclovir. The acyclovir water dispersible tablet was prepared by using Veegum F (Magnesium Aluminum Silicate) and Sodium Starch Glycolate as superdisintegrating agent, Avicel PH 102 as a diluent, Povidone K30 as a binder and carried out studies for weight variation, thickness, hardness, content uniformity, disintegrating time, dispersion time, wetting time and in vitro drug release. Tablets were prepared by using wet granulation method. Out of five, formulation F2 exhibited highest drug release 85.11% and formulation F4 showed drug release 85.42% in 10 min. They also exhibited the shortest wetting time 57.56 and 54.46 second with dispersion time 78 and 110 second respectively. It was concluded that water dispersible tablets with enhanced dissolution rate can be made using selective superdisintegrants.