Slawomir Pikula - Academia.edu (original) (raw)

Papers by Slawomir Pikula

Cardiovascular Research, 2020

Cardiovascular calcification (CVC) is associated with increased morbidity and mortality. It devel... more Cardiovascular calcification (CVC) is associated with increased morbidity and mortality. It develops in several diseases and locations, such as in the tunica intima in atherosclerosis plaques, in the tunica media in type 2 diabetes and chronic kidney disease, and in aortic valves. In spite of the wide occurrence of CVC and its detrimental effects on cardiovascular diseases (CVD), no treatment is yet available. Most of CVC involve mechanisms similar to those occurring during endochondral and/or intramembranous ossification. Logically, since tissue-nonspecific alkaline phosphatase (TNAP) is the key-enzyme responsible for skeletal/dental mineralization, it is a promising target to limit CVC. Tools have recently been developed to inhibit its activity and preclinical studies conducted in animal models of vascular calcification already provided promising results. Nevertheless, as its name indicates, TNAP is ubiquitous and recent data indicate that it dephosphorylates different substrates ...

Journal of Extracellular Biology

Figure S1. Implementation of apparatus for macroscopic imaging using light-sheet illumination. (a... more Figure S1. Implementation of apparatus for macroscopic imaging using light-sheet illumination. (a) General view. (b) Side view. (c) Front view. (1) camera, (2) stepper motor, (3) z-axis line module (here light microscope body, Zeiss), (4) base holder, (5) glass container, (6) line lasers, (7) metal block, (8) laser power supply. Figure S2. Perfusion-based CUBIC cleared rat organs stained with propidium iodide. Bright field images of whole rat organs (a) intestine, (b) kidney, (c) heart, (d) cerebellum and (e) spleen. Single squares in all panels - 5 × 5 mm. This figure has been adapted from the original article "Optimized perfusion-based CUBIC protocol for the efficient whole-body clearing and imaging of rat organs" by P. Matryba et al., J Biophotonics 2017, doi./10.1002/jbio.201700248 . Reproduced with permission 4358370973288. Figure S3. Comparison between optical and standard histopathology sectioning of Alizarin red S stained mdx mouse muscles. (a) Representative optic...

The roles of annexins and alkaline phosphatase in mineralization process �

International Journal of Molecular Sciences, 2021

The mineralization process is initiated by osteoblasts and chondrocytes during intramembranous an... more The mineralization process is initiated by osteoblasts and chondrocytes during intramembranous and endochondral ossifications, respectively. Both types of cells release matrix vesicles (MVs), which accumulate Pi and Ca2+ and form apatites in their lumen. Tissue non-specific alkaline phosphatase (TNAP), a mineralization marker, is highly enriched in MVs, in which it removes inorganic pyrophosphate (PPi), an inhibitor of apatite formation. MVs then bud from the microvilli of mature osteoblasts or hypertrophic chondrocytes and, thanks to the action of the acto-myosin cortex, become released to the extracellular matrix (ECM), where they bind to collagen fibers and propagate mineral growth. In this report, we compared the mineralization ability of human fetal osteoblastic cell line (hFOB 1.19 cells) with that of osteosarcoma cell line (Saos-2 cells). Both types of cells were able to mineralize in an osteogenic medium containing ascorbic acid and beta glycerophosphate. The composition of ...

Acta Biochimica Polonica, 2000

Numerous results on membrane lipid composition from different regions of autopsied Alzheimer'... more Numerous results on membrane lipid composition from different regions of autopsied Alzheimer's disease brains in comparison with corresponding fractions isolated from control brains revealed significant differences in serine- and ethanolamine-containing glycerophospholipid as well as in glycosphingolipid content. Changes in membrane lipid composition are frequently accompanied by alterations in membrane fluidity, hydrophobic mismatch, lipid signaling pathways, transient formation and disappearance of lipid microdomains, changes in membrane permeability to cations and variations of other membrane properties. In this review we focus on possible implications of altered membrane composition on beta-amyloid precursor protein (APP) and on proteolysis of APP leading eventually to the formation of neurotoxic beta-amyloid (A beta) peptides, the major proteinaceous component of extracellular senile plaques, directly involved in Alzheimer's disease pathogenesis.

Acta Biochimica Polonica, 2007

Annexin A6 (AnxA6) is a Ca(2+)-dependent membrane-binding protein involved in vesicular traffic. ... more Annexin A6 (AnxA6) is a Ca(2+)-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca(2+) signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca(2+)] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca(2+)] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca(2+)] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation o...

Archives of Biochemistry and Biophysics, 2019

Archives of Biochemistry and Biophysics, 2016

Mutations in the NPC1 or NPC2 genes lead to Niemann-Pick type C (NPC) disease, a rare lysosomal s... more Mutations in the NPC1 or NPC2 genes lead to Niemann-Pick type C (NPC) disease, a rare lysosomal storage disorder characterized by progressive neurodegeneration. These mutations result in cholesterol and glycosphingolipid accumulation in the late endosomal/lysosomal compartment. Complications in the storage of cholesterol in NPC1 mutant cells are associated with other anomalies, such as altered distribution of intracellular organelles and properties of the plasma membrane. The pathomechanism of NPC disease is largely unknown. Interestingly, other storage diseases such as Gaucher and Farber diseases are accompanied by severe mitochondrial dysfunction. This prompted us to investigate the effect of absence or dysfunction of the NPC1 protein on mitochondrial properties to confirm or deny a putative relationship between NPC1 mutations and mitochondrial function. This study was performed on primary skin fibroblasts derived from skin biopsies of two NPC patients, carrying mutations in the NPC1 gene. We observed altered organization of mitochondria in NPC1 mutant cells, significant enrichment in mitochondrial cholesterol content, increased respiration, altered composition of the respiratory chain complex, and substantial reduction in cellular ATP level. Thus, a primary lysosomal defect in NPC1 mutant fibroblasts is accompanied by deregulation of the organization and function of the mitochondrial network.

Nuclear Magnetic Resonance, 2015

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Postepy biochemii, 2012

Cell integrity, assured by plasma membrane continuity, is essential to maintain proper cell funct... more Cell integrity, assured by plasma membrane continuity, is essential to maintain proper cell functioning and survival. Plasma membrane separates the cell interior from the extracellular milieu and constitutes a barrier due to which the spatial relationship between organelles and the internal membrane network as well as the chemical composition of the cytoplasm are preserved during the cellular life span. Therefore, all cellular processes including intracellular ion homeostasis, exchange of substances between the extracellular environment and the cytoplasm, maintenance of cellular shape, cellular movement, vesicular traffic, cell division and membrane biogenesis, as well as and cellular signaling depend on the integrity, structure and function of the plasma membrane. In the course of these processes the plasma membrane is subjected to dynamic changes that can create a kind of mechanical stress and be a source of cell-threatening injuries. These membrane injuries could be also created ...

Nuclear Magnetic Resonance, 2014

This chapter reviews articles on NMR and lipids published between June 2012 and May 2013. The num... more This chapter reviews articles on NMR and lipids published between June 2012 and May 2013. The number of papers devoted to NMR utilization to investigate lipids, their structures, behavior in native and artificial membranes, interactions with proteins and peptides, as well as with low molecular weight molecules, biomedical applications and new methods is growing (over 1300 articles in Pub-Med) although we included here only a selection of those papers that were accessible and peer-reviewed. The reviewed material has been arranged in chapters devoted to the structure and function of lipids in membranes, their roles in membrane-related processes including lipid-mediated signal transduction, interactions of lipids with membrane and soluble proteins, peptides and various low molecular weight compounds, lipid metabolomics, visualization of lipid related processes in biomedicine, lipid-based diagnosis, and methodological approaches.