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Papers by Sneha Choudhary

Research paper thumbnail of An Insight on Mycoflora Associated with Rice Grain Discolouration

Journal of scientific research and reports, May 17, 2024

Grain discolouration, a complex disease of rice is a new enemy to rice crops around the world, an... more Grain discolouration, a complex disease of rice is a new enemy to rice crops around the world, and it is becoming increasingly important due to the qualitative as well as quantitative loss of harvested crop. The present study was carried out on the ten different variety of rice (

Research paper thumbnail of Dissecting brown planthopper resistance genes in Oryza and its wild relatives: A review

Research paper thumbnail of Response of Silkworm (Bombyx mori L.) Breeds to Temperature and BmNPV Stress

International Journal of Environment and Climate Change

Silkworm Bombyx mori L. is one of the most important domesticated insects, which produces luxuria... more Silkworm Bombyx mori L. is one of the most important domesticated insects, which produces luxuriant silk thread in the form of cocoon by consuming mulberry leaves during larval period. The growth and development of silkworm is greatly influenced by environmental conditions. The seasonal differences in the environmental components considerably affect the genotypic expression in the form of phenotypic output of silkworm crop. Bombyx mori nucleopolyhedrovirus (BmNPV) is a pathogen that causes great economic losses in sericulture. In the present investigation, twelve bivoltine silkworm breeds (WM, ND5, NB4D2, U-4, PO1, ND3, U-6, CSR2, SH6, SPO, U-3 and NSP) were studied for temperature and BmNPV stress. After third and fourth moult silkworm larvae were treated with BmNPV inoculum at 25±1⁰C and 30±1⁰C temperature respectively. Increased mortality was observed at 30±1⁰C when compared to 25±1⁰C. However, with respect to breeds the highest total mortality was recorded in breed PO1 and leas...

Research paper thumbnail of Sources of Growth in Cereals Production in Uttar Pradesh, India

The study was conducted in the state Uttar Pradesh, which was selected purposively for the presen... more The study was conducted in the state Uttar Pradesh, which was selected purposively for the present study due to my personal interest. Keeping in mind the agro climatic conditions of the state was divided into nine agro-climatic zones. The methodology used was based on dynamicity of time and regionalization of area, production and yield of major cereal crops grown in Uttar Pradesh viz., rice, maize, pearl millet, sorghum, wheat and barley. The study reveals the positive growth in area, production and productivity of rice, wheat and maize whereas other crops showed the mixed trend. The variability was found highest in production followed by productivity and area throughout the years. The total production of cereals as per the study was due to the increase in production, area and its interaction with other factors. The estimation of future requirement shows that we are self sufficient in the case of cereals production and no need to borrow from other state or import for the consumption.

Research paper thumbnail of Presence of polyadenylated 3′ tail in RNA of Pepper mild mottle virus allows the Oligo(dT)18 in Priming cDNA Synthesis of its genomic RNA template

The PCR amplification of majority of the ssRNA of both genomic and non-genomic mRNA is accomplish... more The PCR amplification of majority of the ssRNA of both genomic and non-genomic mRNA is accomplished by RT-PCR. The mRNA is subjected to cDNA synthesis using reverse transcriptase and either Oligo(dT)18, or random or gene specific reverse primers based priming strategies. The choice of primer largely depends on the nature of 3 prime terminus of mRNA and length of cDNA synthesized. In general, oligo(dT)18 is the preferred choice for mRNAs having poly(A) tail at 3 prime terminus. In general, tobamoviruses lack any poly(A) tail at their 3 prime untranslated region (UTR) which forms a tRNA like structure and upstream pseudoknot domain except tow viruses viz., Hibiscus latent Fort Pierce virus (HLFPV) and Hibiscus latent Singapore virus (HLSV) which accommodate internal poly(A) sequences of 46 and 87 nucleotides long, respectively in their 3 prime UTR. However, determination of full nucleotide sequence of Pepper mild mottle virus (PMMoV) using an oligo(dT)18 primed cDNA as template indicated the libertinism of oligo(dT)18 in priming cDNA synthesis of RNA template which are known to lack poly(A) tail. at the end or internally at its 3 prime end. Moreover, coat protein (CP) gene of PMMoV and bean common mosaic virus (BCMV) (Potyvirus with a poly(A) tract at its 3 prime end) was amplified using cDNA primed with random primer as well as oligo(dT)18 was successfully amplified but with significant variation in the intensity of the amplification band in case of PMMoV but not in BCMV. This clearly indicated the presence of PMMoV mRNA with polyadenylated 3 prime tail in total RNA isolated from PMMoV infected capsicum leaves with abundance of non-polyadenylated PMMoV genomic RNA (gRNA). Hence, we hypothesize that the generation of polyadenylated RNA population during the infection cycle of PMMoV in pepper may be possible reason for libertinism of oligo(dT)18 in priming cDNA synthesis of RNA template isolated from PMMoV infected leaves followed by amplification of entire PMMoV genome through RT-PCR. This is first study indicating the presence of polyadenylated or polyadenylated rich regions in PMMoV gRNA acquired during the infection cycle in pepper.

Research paper thumbnail of An Insight on Mycoflora Associated with Rice Grain Discolouration

Journal of scientific research and reports, May 17, 2024

Grain discolouration, a complex disease of rice is a new enemy to rice crops around the world, an... more Grain discolouration, a complex disease of rice is a new enemy to rice crops around the world, and it is becoming increasingly important due to the qualitative as well as quantitative loss of harvested crop. The present study was carried out on the ten different variety of rice (

Research paper thumbnail of Dissecting brown planthopper resistance genes in Oryza and its wild relatives: A review

Research paper thumbnail of Response of Silkworm (Bombyx mori L.) Breeds to Temperature and BmNPV Stress

International Journal of Environment and Climate Change

Silkworm Bombyx mori L. is one of the most important domesticated insects, which produces luxuria... more Silkworm Bombyx mori L. is one of the most important domesticated insects, which produces luxuriant silk thread in the form of cocoon by consuming mulberry leaves during larval period. The growth and development of silkworm is greatly influenced by environmental conditions. The seasonal differences in the environmental components considerably affect the genotypic expression in the form of phenotypic output of silkworm crop. Bombyx mori nucleopolyhedrovirus (BmNPV) is a pathogen that causes great economic losses in sericulture. In the present investigation, twelve bivoltine silkworm breeds (WM, ND5, NB4D2, U-4, PO1, ND3, U-6, CSR2, SH6, SPO, U-3 and NSP) were studied for temperature and BmNPV stress. After third and fourth moult silkworm larvae were treated with BmNPV inoculum at 25±1⁰C and 30±1⁰C temperature respectively. Increased mortality was observed at 30±1⁰C when compared to 25±1⁰C. However, with respect to breeds the highest total mortality was recorded in breed PO1 and leas...

Research paper thumbnail of Sources of Growth in Cereals Production in Uttar Pradesh, India

The study was conducted in the state Uttar Pradesh, which was selected purposively for the presen... more The study was conducted in the state Uttar Pradesh, which was selected purposively for the present study due to my personal interest. Keeping in mind the agro climatic conditions of the state was divided into nine agro-climatic zones. The methodology used was based on dynamicity of time and regionalization of area, production and yield of major cereal crops grown in Uttar Pradesh viz., rice, maize, pearl millet, sorghum, wheat and barley. The study reveals the positive growth in area, production and productivity of rice, wheat and maize whereas other crops showed the mixed trend. The variability was found highest in production followed by productivity and area throughout the years. The total production of cereals as per the study was due to the increase in production, area and its interaction with other factors. The estimation of future requirement shows that we are self sufficient in the case of cereals production and no need to borrow from other state or import for the consumption.

Research paper thumbnail of Presence of polyadenylated 3′ tail in RNA of Pepper mild mottle virus allows the Oligo(dT)18 in Priming cDNA Synthesis of its genomic RNA template

The PCR amplification of majority of the ssRNA of both genomic and non-genomic mRNA is accomplish... more The PCR amplification of majority of the ssRNA of both genomic and non-genomic mRNA is accomplished by RT-PCR. The mRNA is subjected to cDNA synthesis using reverse transcriptase and either Oligo(dT)18, or random or gene specific reverse primers based priming strategies. The choice of primer largely depends on the nature of 3 prime terminus of mRNA and length of cDNA synthesized. In general, oligo(dT)18 is the preferred choice for mRNAs having poly(A) tail at 3 prime terminus. In general, tobamoviruses lack any poly(A) tail at their 3 prime untranslated region (UTR) which forms a tRNA like structure and upstream pseudoknot domain except tow viruses viz., Hibiscus latent Fort Pierce virus (HLFPV) and Hibiscus latent Singapore virus (HLSV) which accommodate internal poly(A) sequences of 46 and 87 nucleotides long, respectively in their 3 prime UTR. However, determination of full nucleotide sequence of Pepper mild mottle virus (PMMoV) using an oligo(dT)18 primed cDNA as template indicated the libertinism of oligo(dT)18 in priming cDNA synthesis of RNA template which are known to lack poly(A) tail. at the end or internally at its 3 prime end. Moreover, coat protein (CP) gene of PMMoV and bean common mosaic virus (BCMV) (Potyvirus with a poly(A) tract at its 3 prime end) was amplified using cDNA primed with random primer as well as oligo(dT)18 was successfully amplified but with significant variation in the intensity of the amplification band in case of PMMoV but not in BCMV. This clearly indicated the presence of PMMoV mRNA with polyadenylated 3 prime tail in total RNA isolated from PMMoV infected capsicum leaves with abundance of non-polyadenylated PMMoV genomic RNA (gRNA). Hence, we hypothesize that the generation of polyadenylated RNA population during the infection cycle of PMMoV in pepper may be possible reason for libertinism of oligo(dT)18 in priming cDNA synthesis of RNA template isolated from PMMoV infected leaves followed by amplification of entire PMMoV genome through RT-PCR. This is first study indicating the presence of polyadenylated or polyadenylated rich regions in PMMoV gRNA acquired during the infection cycle in pepper.