Soerya Marliyana - Academia.edu (original) (raw)
Papers by Soerya Marliyana
Journal of Biomolecular Structure and Dynamics
IOP Conference Series: Materials Science and Engineering, 2019
Antibacterial activity of polypugenol compounds has been carried out in the form active ingredien... more Antibacterial activity of polypugenol compounds has been carried out in the form active ingredients and gels against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Antibacterial activity assay of polyeugenol in active form with varied concentration 0.0025 g/mL, 0.005 g/mL, 0.01 g/mL, 0.02 g/mL, and 0.04 g/mL and gelling form varied concentration 0.01 g/mL, 0.02 g/mL, and 0.04 g/mL. Antibacterial activity assay using agar disk diffusion method by measuring the diameter of inhibition.The result showed that antibacterial activity not presence at Escherichia coli while Staphylococcus aureus has presence of antibacterial activity at concentration 0.01 g/mL, 0.02 g/mL, dan 0.04 g/mL with inhibition diameter values 15.72; 17.28 and 20.51 mm. Polyeugenol in gelling form were tested against Staphylococcus aureus and Escherichia coli at concentration 0.01g/mL, 0.02g/mL, dan 0.04g/mL. The presence of antibacterial activity obtained for Escherichia coli only at concentration 0.04 g/mL with the value of inhibition diameter is 0.53 mm while concentration 0.01g/mL dan 0.02g/mL not presence antibacterial activity. The presence of antibacterial activity for Staphylococcus aureus obtained at concentration 0.01 g/mL, 0.02 g/mL and 0.04 g/mL with the value of inhibition diameter are 14.46; 15.68 and 18.81 mm that showed relatively strong antibacterial activity. Statistic tested using one way ANOVA in Staphylococcus aureus shows significant differences at each concentration from polyeugenol in active form and gelling form with F-count> F-tab.
![Research paper thumbnail of Synthesis and Structure Characterization of Copper(II) Complexes with 5,5'-Dihydroxy-2,2'-[ethylenedioxybis(nitrilomethylidyne)]diphenol](https://attachments.academia-assets.com/96449304/thumbnails/1.jpg)
](Asian Journal of Chemistry, 2013
A new tetradentate bis-oxime chelating ligand, 5,5'-dihydroxy-2,2'-[ethylenedioxybis(nitrilomethy... more A new tetradentate bis-oxime chelating ligand, 5,5'-dihydroxy-2,2'-[ethylenedioxybis(nitrilomethylidyne)]diphenol (H4L) and its two Cu(II) complexes 1 and 2 have been synthesized by the reaction of H4L with copper(II) acetate monohydrate and copper(II) picrate tetrahydrate, respectively. The complexes have been characterized by elemental analyses, IR spectra, UV-visible spectra, TG-DTA and molar conductances. The salen-type bisoxime chelating ligand is a good tetradentate N2O2-donating ligand and the likely formulae of the Cu(II) complexes may be suggested as [Cu(H2L)]•CH3CH2OH•2H2O (1) and [Cu2(H2L)(picrate)2]•H2O (2).
Journal of Physics: Conference Series, 2019
IOP Conference Series: Materials Science and Engineering, 2018
Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "T... more Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "TemuKunci"in Indonesia, is one of the medicinal plants of the family Zingiberaceae. Phytochemical studies on the rhizome of K. pandurata showed the presence of flavonoid derivative, namely flavanones, which constitute as the main components of this plant. Bioactivity studies on this species exhibited various biological activities, such as antibacteria, anti-inflammatory, antitumor, antidiarrhea, antidisentri, anti-HIV, antioxidant, antipyretic, analgesic and insecticides. Among the biological activities, the antibacterial activity results are important as an attempt to answer the emergence of resistance of some bacteria against existing drugs, as well as the emergence of a number of outbreaks of disease caused by bacteria. Therefore, a search to find new compounds that are potential as an antibacterial is an urgent matter. The present study was aimed at the chemical transformation of pinostrobin (1) from K. pandurata rhizome and an antibacterial activity.The chemical transformation was performed through a prenylation reaction of pinostrobin (1) which is the main component of K. pandurata rhizome. The prenylation reaction was carried out by reacting pinostrobin (1) with prenyl bromide and potassium carbonat (K2CO3). The purification of product was done using the radial chromatography with mix solvent n-hexane and ethyl acetate (97.5:2.5; 9.5:0.5; 9.0:1.0.; 8.0:2.0). The purity test of isolated compound was analysedby TLC using different types of eluent. The identification of compounds was determined based on NMR data and mass spectra analysis. Five compounds were obtained from the prenylation reaction, i.e. monooxyprenylated pinostrobin (2), monooxyprenylated chalcone (3), diprenylated chalcone (4), triprenylated chalcone (5), and triprenylated cyclohexene chalcone (6). These compounds were tested for antibacterial activities against four clinical bacteria, namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The antibacterial test was conducted using microdilution method. The five compounds showed moderate antibacterial activity against clinical bacteria with MIC values in the range of 25 to 50 µg/mL.
IOP Conference Series: Materials Science and Engineering, 2018
Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "T... more Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "TemuKunci"in Indonesia, is one of the medicinal plants of the family Zingiberaceae. Phytochemical studies on the rhizome of K. pandurata showed the presence of flavonoid derivative, namely flavanones, which constitute as the main components of this plant. Bioactivity studies on this species exhibited various biological activities, such as antibacteria, anti-inflammatory, antitumor, antidiarrhea, antidisentri, anti-HIV, antioxidant, antipyretic, analgesic and insecticides. Among the biological activities, the antibacterial activity results are important as an attempt to answer the emergence of resistance of some bacteria against existing drugs, as well as the emergence of a number of outbreaks of disease caused by bacteria. Therefore, a search to find new compounds that are potential as an antibacterial is an urgent matter. The present study was aimed at the chemical transformation of pinostrobin (1) from K. pandurata rhizome and an antibacterial activity.The chemical transformation was performed through a prenylation reaction of pinostrobin (1) which is the main component of K. pandurata rhizome. The prenylation reaction was carried out by reacting pinostrobin (1) with prenyl bromide and potassium carbonat (K2CO3). The purification of product was done using the radial chromatography with mix solvent n-hexane and ethyl acetate (97.5:2.5; 9.5:0.5; 9.0:1.0.; 8.0:2.0). The purity test of isolated compound was analysedby TLC using different types of eluent. The identification of compounds was determined based on NMR data and mass spectra analysis. Five compounds were obtained from the prenylation reaction, i.e. monooxyprenylated pinostrobin (2), monooxyprenylated chalcone (3), diprenylated chalcone (4), triprenylated chalcone (5), and triprenylated cyclohexene chalcone (6). These compounds were tested for antibacterial activities against four clinical bacteria, namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The antibacterial test was conducted using microdilution method. The five compounds showed moderate antibacterial activity against clinical bacteria with MIC values in the range of 25 to 50 µg/mL.
THE 14TH JOINT CONFERENCE ON CHEMISTRY 2019
Biodiversitas Journal of Biological Diversity, 2022
A study was performed on the antioxidant activity of the methanol extract of Luffa acutangula (L.... more A study was performed on the antioxidant activity of the methanol extract of Luffa acutangula (L.) Roxb (ridge gourd) fruit (LAM) and its derived fractions, such as n-hexane (LAH), chloroform (LAC), ethyl acetate (LAE), n-butanol (LAB) and residual aqueous fraction (LAA) and their correlation to the total phenolics and flavonoids contents. The phytochemical screening demonstrated the presence of phenolic and flavonoid compounds in all the fractions except the n-hexane fraction. Carotenoids were present in the LAH and LAC, while absent in the LAE. The antioxidant activity was assessed using β-carotene bleaching method and the results showed that LAM, LAH and LAC have significant antioxidant activities in the comparison with those of BHT and PG. The total phenolics content of the extract/fractions was determined by the Folin-Ciocalteu procedure and ranged from 18.7±0.11 to 105.1±0.08 mg GAE/g of dried weight basis. The flavonoids content of extract/fractions was measured by aluminium ...
IOP Conference Series: Materials Science and Engineering, 2020
Microcrystalline cellulose was isolated from the banana stem, an abundant but underutilized biore... more Microcrystalline cellulose was isolated from the banana stem, an abundant but underutilized bioresource, by using three main steps, specifically bleaching, alkaline treatment and acid hydrolysis. The isolated MC was used to reinforce starch-based biofoams with different loadings of 0%, 2%, 4%, and 6%. The result of contact angle measurement using the LB-ADSA method achieved remarkable increases of contact angle value as the MC loading increased from 0% to 6%. In addition, compared to starch-based biofoams, starch/MC composite biofoams showed improved biodegradability. The starch/MC composite biofoams displayed good hydrophobicity properties that can potentially represent a sustainable and green alternative for packaging and insulation materials.
IOP Conference Series: Materials Science and Engineering, 2017
The phytochemical screening conducted on ethanol extract of Luffa acutangula (L.) Roxb's fruit re... more The phytochemical screening conducted on ethanol extract of Luffa acutangula (L.) Roxb's fruit revealed the presence of alkaloids, saponins, carotenoids and terpenoids and the absence of flavonoids, tannins and anthraquinones. The GC-MS of the analysis L. acutangula (L.) Roxb's fraction resulted in the identification of six compounds. The compounds that could be identified were 2,3-dihydro,3,5-dihydroxy-6-methyl-(4H)-pyran-4-one; 3,7,11,15tetramethyl-2-hexadecen-1-ol; (3β, 20R)-cholest-5-en-3-ol; n-hexadecanoic acid; 9, 12, 15octadecatrienoic acid methyl ester and citronellyl tiglate. The present study provides evidence that L. acutangula's fruit contains medicinally important bioactive compounds and this justifies the possibly use of these fruits as traditional medicine for treatment of various diseases.
Indonesian Journal of Chemistry, 2013
Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flouresce... more Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70% when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soybe...
AIP Conference Proceedings, 2015
A molasses based medium for the biosurfactant production by Pseudomonas fluorescens was developed... more A molasses based medium for the biosurfactant production by Pseudomonas fluorescens was developed, where the effect of pre-treated of molasses and medium composition were evaluated. Biosurfactant production was followed by measuring optical density (OD), surface tension and emulsifying index (E24) over 12 days of fermentation. The optimum condition for the biosurfactant production was obtained when a medium containing of 8 g/L nutrient broth, 5 g/L NaCl, 1 g/L NH4NO3 and 5% v/v pre-treated molasses with centrifugation was used as media with 3 days of fermentation. The biosurfactant was identified as a rhamnolipid type biosurfactant which had critical micelle concentration (CMC) value of 801 mg/L and was able to reduce the surface tension of the water from 80 mN/m to 51 mN/m. The biosurfactants had water in oil (w/o) emulsion type. Biosurfactant was able to emulsify various hydrocarbons, which were able to decrase the interfacial tension about 50-75% when benzyl chloride, anisaldehyde and palm oil were used as immiscible compounds. The biosurfactant exhibited the E24 value of about 50% and the stable emulsion was reached up to 30 days when lubricant was used as an immiscible compound. Up to 68% of phenol was degraded in the presence of biosurfactant within 15 days, whereas only 56% of phenol was degraded in the absence of biosurfactant. Overall, the results exhibited that molasses are recommended for the rhamnolipids production which possessed good surface-active properties and had potential application in the enhancement of phenol degradation.
ALCHEMY Jurnal Penelitian Kimia, 2017
Telah dilakukan uji aktivitas secara in vitro terhadap bakteri isolat klinis dari senyawa turunan... more Telah dilakukan uji aktivitas secara in vitro terhadap bakteri isolat klinis dari senyawa turunan calkon yang berhasil diisolasi dari rimpang temu kunci (K. pandurata). Dua senyawa turunan calkon, panduratin A (1) dan 4-hidroksipanduratin A (2) telah berhasil diisolasi dari rimpang K. pandurata. Isolasi dilakukan dengan metode ekstraksi (maserasi), kromatografi kolom vakum cair dan kromatografi radial. Identifikasi struktur dilakukan dengan metode spektroskopi NMR (1 H NMR, 13 C NMR, 1D dan 2D) dan dibandingkan dengan data literatur yang telah dilaporkan. Uji aktivitas antibakteri dilakukan dengan metode mikrodilusi mengacu pada CLSI, terhadap delapan bakteri isolat klinis yaitu Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae dan Vibrio cholerae. Senyawa 1 dan 2 menunjukkan aktivitas antibakteri yang signifikan terhadap bakteri uji, dengan aktivitas tertinggi terhadap S.aureus dan B. subtilis dengan nilai MIC sebesar 2,4-18,8 µg/mL dan MBC pada kisaran 4,8-37,5 µg/mL. Hasi uji antibakteri menunjukkan kedua senyawa tersebut potensial sebagai senyawa antibakteri terhadap bakteri isolat klinis. Kata kunci: antibakteri, bakteri isolat klinis, 4-hidroksipanduratin A, panduratin A.
Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flouresce... more Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70 % when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soyb...
Molekul
Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a tra... more Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopher...
Atomic Abso p rtion Spektrophotomet er (AAS) atau Spektrofotomet er Serapan Atom adalah istrumen ... more Atomic Abso p rtion Spektrophotomet er (AAS) atau Spektrofotomet er Serapan Atom adalah istrumen untuk melakukan analisa logam antara lain : Pb, Cr, Ni, Cd, Fe, Zn, Cu, dan Co dimana dasar pengukurannya adalah pengukuran serapan sinar oleh suatu atom. Sinar yang tidak diserap diteruskan dan dirubah menjadi sinar listrik yang terukur Tujuan Penelitian ini adalah mengetahui perform a alat AAS Therma ICE 3000 terhadap pengujian logam Pb. Hal ini sangat penting karena berpengaruh besar terhadap validitas hasil pengukuran pada kegiatan riset,praktikum dan pengujian di Laboratorium Kimia UPT Lab Terpadu UNS. Perform a yang akan diteliti meliputi akurasi, presisi, limit deteksi dan liniaritas. Akurasi dilakukan dengan pengukuran CRM 500 dan 697, presisi dilakukan dengan mengukur standar 10 pengulangan, limit deteksi dengan mengukur larutan blangko 10 pengulangan, liniaritas dilakukan dengan mengukur larutan baku dari berbagai deret konsentrasi. Dari pengukuran didapatkan hasil uji linearit...
A study was performed on the antioxidant activity o f the methanol extract of Luffa acutangula (L... more A study was performed on the antioxidant activity o f the methanol extract of Luffa acutangula (L.) Rox b (ridge gourd) fruit (LAM) and its derived fractions, such as n-hexane (LAH), chloroform (LAC), ethyl acetate (LAE), nbutanol (LAB) and residual aqueous fraction (LAA) a nd their correlation to the total phenolics and fla vonoids contents. The phytochemical screening demonstrated the presence of phenolic and flavonoid compounds in all the fractions except the n-hexane fraction. Carotenoids were present in the LAH and LAC, while absent in t he LAE. The antioxidant activity was assessed using β-carotene bleaching method and the results showed t hat LAM, LAH and LAC have significant antioxidant activities in the c omparison with those of BHT and PG. The total pheno lics content of the extract/fractions was determined by the Foli n-C ocalteu procedure and ranged from 18.7±0.11 to 105.1±0.08 mg GAE/g of dried weight basis. The flavonoids cont ent of extract/fractions was measured by a...
Indonesian Journal of Chemistry, 2021
The novel nickel(II) complex has been successfully synthesized through the reaction of Ni(NO 3 ) ... more The novel nickel(II) complex has been successfully synthesized through the reaction of Ni(NO 3 ) 2 ·6H 2 O with 4-aminoantipyrine (AAP) ligand in a 1:3 mole ratio of Ni(II) to AAP. The complex was characterized using UV-Vis, Atomic Absorption Spectroscopy (AAS), Infrared spectrophotometry (IR), Thermogravimetry/Differential Scanning Calorimetry (TG/DSC), conductivity, and magnetic susceptibility. The complex formula was [Ni(AAP) 3 ](NO 3 ) 2 ·5H 2 O. AAP was a bidentate ligand that coordinated through the primary amine nitrogen and the carbonyl oxygen to the nickel ion. The electronic spectra of the complex displayed two peaks at 646 nm and 385 nm in accordance with the 3 A 2g (F) → 3 T 1g (F) and 3 A 2g (F) → 3 T 1g (P) transitions, respectively. This complex gave a paramagnetic property with the effective magnetic moment (µ eff ) of 2.96 BM and the shape of an octahedron. The antibacterial test of this complex showed higher activity than the metal and its free ligand.
Journal of Biomolecular Structure and Dynamics
IOP Conference Series: Materials Science and Engineering, 2019
Antibacterial activity of polypugenol compounds has been carried out in the form active ingredien... more Antibacterial activity of polypugenol compounds has been carried out in the form active ingredients and gels against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Antibacterial activity assay of polyeugenol in active form with varied concentration 0.0025 g/mL, 0.005 g/mL, 0.01 g/mL, 0.02 g/mL, and 0.04 g/mL and gelling form varied concentration 0.01 g/mL, 0.02 g/mL, and 0.04 g/mL. Antibacterial activity assay using agar disk diffusion method by measuring the diameter of inhibition.The result showed that antibacterial activity not presence at Escherichia coli while Staphylococcus aureus has presence of antibacterial activity at concentration 0.01 g/mL, 0.02 g/mL, dan 0.04 g/mL with inhibition diameter values 15.72; 17.28 and 20.51 mm. Polyeugenol in gelling form were tested against Staphylococcus aureus and Escherichia coli at concentration 0.01g/mL, 0.02g/mL, dan 0.04g/mL. The presence of antibacterial activity obtained for Escherichia coli only at concentration 0.04 g/mL with the value of inhibition diameter is 0.53 mm while concentration 0.01g/mL dan 0.02g/mL not presence antibacterial activity. The presence of antibacterial activity for Staphylococcus aureus obtained at concentration 0.01 g/mL, 0.02 g/mL and 0.04 g/mL with the value of inhibition diameter are 14.46; 15.68 and 18.81 mm that showed relatively strong antibacterial activity. Statistic tested using one way ANOVA in Staphylococcus aureus shows significant differences at each concentration from polyeugenol in active form and gelling form with F-count> F-tab.
Asian Journal of Chemistry, 2013
A new tetradentate bis-oxime chelating ligand, 5,5'-dihydroxy-2,2'-[ethylenedioxybis(nitrilomethy... more A new tetradentate bis-oxime chelating ligand, 5,5'-dihydroxy-2,2'-[ethylenedioxybis(nitrilomethylidyne)]diphenol (H4L) and its two Cu(II) complexes 1 and 2 have been synthesized by the reaction of H4L with copper(II) acetate monohydrate and copper(II) picrate tetrahydrate, respectively. The complexes have been characterized by elemental analyses, IR spectra, UV-visible spectra, TG-DTA and molar conductances. The salen-type bisoxime chelating ligand is a good tetradentate N2O2-donating ligand and the likely formulae of the Cu(II) complexes may be suggested as [Cu(H2L)]•CH3CH2OH•2H2O (1) and [Cu2(H2L)(picrate)2]•H2O (2).
Journal of Physics: Conference Series, 2019
IOP Conference Series: Materials Science and Engineering, 2018
Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "T... more Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "TemuKunci"in Indonesia, is one of the medicinal plants of the family Zingiberaceae. Phytochemical studies on the rhizome of K. pandurata showed the presence of flavonoid derivative, namely flavanones, which constitute as the main components of this plant. Bioactivity studies on this species exhibited various biological activities, such as antibacteria, anti-inflammatory, antitumor, antidiarrhea, antidisentri, anti-HIV, antioxidant, antipyretic, analgesic and insecticides. Among the biological activities, the antibacterial activity results are important as an attempt to answer the emergence of resistance of some bacteria against existing drugs, as well as the emergence of a number of outbreaks of disease caused by bacteria. Therefore, a search to find new compounds that are potential as an antibacterial is an urgent matter. The present study was aimed at the chemical transformation of pinostrobin (1) from K. pandurata rhizome and an antibacterial activity.The chemical transformation was performed through a prenylation reaction of pinostrobin (1) which is the main component of K. pandurata rhizome. The prenylation reaction was carried out by reacting pinostrobin (1) with prenyl bromide and potassium carbonat (K2CO3). The purification of product was done using the radial chromatography with mix solvent n-hexane and ethyl acetate (97.5:2.5; 9.5:0.5; 9.0:1.0.; 8.0:2.0). The purity test of isolated compound was analysedby TLC using different types of eluent. The identification of compounds was determined based on NMR data and mass spectra analysis. Five compounds were obtained from the prenylation reaction, i.e. monooxyprenylated pinostrobin (2), monooxyprenylated chalcone (3), diprenylated chalcone (4), triprenylated chalcone (5), and triprenylated cyclohexene chalcone (6). These compounds were tested for antibacterial activities against four clinical bacteria, namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The antibacterial test was conducted using microdilution method. The five compounds showed moderate antibacterial activity against clinical bacteria with MIC values in the range of 25 to 50 µg/mL.
IOP Conference Series: Materials Science and Engineering, 2018
Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "T... more Kaempferia pandurata (syn. Boesenbergia rotunda, B. pandurata (Roxb.)Schltr), locally known as "TemuKunci"in Indonesia, is one of the medicinal plants of the family Zingiberaceae. Phytochemical studies on the rhizome of K. pandurata showed the presence of flavonoid derivative, namely flavanones, which constitute as the main components of this plant. Bioactivity studies on this species exhibited various biological activities, such as antibacteria, anti-inflammatory, antitumor, antidiarrhea, antidisentri, anti-HIV, antioxidant, antipyretic, analgesic and insecticides. Among the biological activities, the antibacterial activity results are important as an attempt to answer the emergence of resistance of some bacteria against existing drugs, as well as the emergence of a number of outbreaks of disease caused by bacteria. Therefore, a search to find new compounds that are potential as an antibacterial is an urgent matter. The present study was aimed at the chemical transformation of pinostrobin (1) from K. pandurata rhizome and an antibacterial activity.The chemical transformation was performed through a prenylation reaction of pinostrobin (1) which is the main component of K. pandurata rhizome. The prenylation reaction was carried out by reacting pinostrobin (1) with prenyl bromide and potassium carbonat (K2CO3). The purification of product was done using the radial chromatography with mix solvent n-hexane and ethyl acetate (97.5:2.5; 9.5:0.5; 9.0:1.0.; 8.0:2.0). The purity test of isolated compound was analysedby TLC using different types of eluent. The identification of compounds was determined based on NMR data and mass spectra analysis. Five compounds were obtained from the prenylation reaction, i.e. monooxyprenylated pinostrobin (2), monooxyprenylated chalcone (3), diprenylated chalcone (4), triprenylated chalcone (5), and triprenylated cyclohexene chalcone (6). These compounds were tested for antibacterial activities against four clinical bacteria, namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The antibacterial test was conducted using microdilution method. The five compounds showed moderate antibacterial activity against clinical bacteria with MIC values in the range of 25 to 50 µg/mL.
THE 14TH JOINT CONFERENCE ON CHEMISTRY 2019
Biodiversitas Journal of Biological Diversity, 2022
A study was performed on the antioxidant activity of the methanol extract of Luffa acutangula (L.... more A study was performed on the antioxidant activity of the methanol extract of Luffa acutangula (L.) Roxb (ridge gourd) fruit (LAM) and its derived fractions, such as n-hexane (LAH), chloroform (LAC), ethyl acetate (LAE), n-butanol (LAB) and residual aqueous fraction (LAA) and their correlation to the total phenolics and flavonoids contents. The phytochemical screening demonstrated the presence of phenolic and flavonoid compounds in all the fractions except the n-hexane fraction. Carotenoids were present in the LAH and LAC, while absent in the LAE. The antioxidant activity was assessed using β-carotene bleaching method and the results showed that LAM, LAH and LAC have significant antioxidant activities in the comparison with those of BHT and PG. The total phenolics content of the extract/fractions was determined by the Folin-Ciocalteu procedure and ranged from 18.7±0.11 to 105.1±0.08 mg GAE/g of dried weight basis. The flavonoids content of extract/fractions was measured by aluminium ...
IOP Conference Series: Materials Science and Engineering, 2020
Microcrystalline cellulose was isolated from the banana stem, an abundant but underutilized biore... more Microcrystalline cellulose was isolated from the banana stem, an abundant but underutilized bioresource, by using three main steps, specifically bleaching, alkaline treatment and acid hydrolysis. The isolated MC was used to reinforce starch-based biofoams with different loadings of 0%, 2%, 4%, and 6%. The result of contact angle measurement using the LB-ADSA method achieved remarkable increases of contact angle value as the MC loading increased from 0% to 6%. In addition, compared to starch-based biofoams, starch/MC composite biofoams showed improved biodegradability. The starch/MC composite biofoams displayed good hydrophobicity properties that can potentially represent a sustainable and green alternative for packaging and insulation materials.
IOP Conference Series: Materials Science and Engineering, 2017
The phytochemical screening conducted on ethanol extract of Luffa acutangula (L.) Roxb's fruit re... more The phytochemical screening conducted on ethanol extract of Luffa acutangula (L.) Roxb's fruit revealed the presence of alkaloids, saponins, carotenoids and terpenoids and the absence of flavonoids, tannins and anthraquinones. The GC-MS of the analysis L. acutangula (L.) Roxb's fraction resulted in the identification of six compounds. The compounds that could be identified were 2,3-dihydro,3,5-dihydroxy-6-methyl-(4H)-pyran-4-one; 3,7,11,15tetramethyl-2-hexadecen-1-ol; (3β, 20R)-cholest-5-en-3-ol; n-hexadecanoic acid; 9, 12, 15octadecatrienoic acid methyl ester and citronellyl tiglate. The present study provides evidence that L. acutangula's fruit contains medicinally important bioactive compounds and this justifies the possibly use of these fruits as traditional medicine for treatment of various diseases.
Indonesian Journal of Chemistry, 2013
Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flouresce... more Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70% when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soybe...
AIP Conference Proceedings, 2015
A molasses based medium for the biosurfactant production by Pseudomonas fluorescens was developed... more A molasses based medium for the biosurfactant production by Pseudomonas fluorescens was developed, where the effect of pre-treated of molasses and medium composition were evaluated. Biosurfactant production was followed by measuring optical density (OD), surface tension and emulsifying index (E24) over 12 days of fermentation. The optimum condition for the biosurfactant production was obtained when a medium containing of 8 g/L nutrient broth, 5 g/L NaCl, 1 g/L NH4NO3 and 5% v/v pre-treated molasses with centrifugation was used as media with 3 days of fermentation. The biosurfactant was identified as a rhamnolipid type biosurfactant which had critical micelle concentration (CMC) value of 801 mg/L and was able to reduce the surface tension of the water from 80 mN/m to 51 mN/m. The biosurfactants had water in oil (w/o) emulsion type. Biosurfactant was able to emulsify various hydrocarbons, which were able to decrase the interfacial tension about 50-75% when benzyl chloride, anisaldehyde and palm oil were used as immiscible compounds. The biosurfactant exhibited the E24 value of about 50% and the stable emulsion was reached up to 30 days when lubricant was used as an immiscible compound. Up to 68% of phenol was degraded in the presence of biosurfactant within 15 days, whereas only 56% of phenol was degraded in the absence of biosurfactant. Overall, the results exhibited that molasses are recommended for the rhamnolipids production which possessed good surface-active properties and had potential application in the enhancement of phenol degradation.
ALCHEMY Jurnal Penelitian Kimia, 2017
Telah dilakukan uji aktivitas secara in vitro terhadap bakteri isolat klinis dari senyawa turunan... more Telah dilakukan uji aktivitas secara in vitro terhadap bakteri isolat klinis dari senyawa turunan calkon yang berhasil diisolasi dari rimpang temu kunci (K. pandurata). Dua senyawa turunan calkon, panduratin A (1) dan 4-hidroksipanduratin A (2) telah berhasil diisolasi dari rimpang K. pandurata. Isolasi dilakukan dengan metode ekstraksi (maserasi), kromatografi kolom vakum cair dan kromatografi radial. Identifikasi struktur dilakukan dengan metode spektroskopi NMR (1 H NMR, 13 C NMR, 1D dan 2D) dan dibandingkan dengan data literatur yang telah dilaporkan. Uji aktivitas antibakteri dilakukan dengan metode mikrodilusi mengacu pada CLSI, terhadap delapan bakteri isolat klinis yaitu Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae dan Vibrio cholerae. Senyawa 1 dan 2 menunjukkan aktivitas antibakteri yang signifikan terhadap bakteri uji, dengan aktivitas tertinggi terhadap S.aureus dan B. subtilis dengan nilai MIC sebesar 2,4-18,8 µg/mL dan MBC pada kisaran 4,8-37,5 µg/mL. Hasi uji antibakteri menunjukkan kedua senyawa tersebut potensial sebagai senyawa antibakteri terhadap bakteri isolat klinis. Kata kunci: antibakteri, bakteri isolat klinis, 4-hidroksipanduratin A, panduratin A.
Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flouresce... more Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70 % when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soyb...
Molekul
Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a tra... more Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopher...
Atomic Abso p rtion Spektrophotomet er (AAS) atau Spektrofotomet er Serapan Atom adalah istrumen ... more Atomic Abso p rtion Spektrophotomet er (AAS) atau Spektrofotomet er Serapan Atom adalah istrumen untuk melakukan analisa logam antara lain : Pb, Cr, Ni, Cd, Fe, Zn, Cu, dan Co dimana dasar pengukurannya adalah pengukuran serapan sinar oleh suatu atom. Sinar yang tidak diserap diteruskan dan dirubah menjadi sinar listrik yang terukur Tujuan Penelitian ini adalah mengetahui perform a alat AAS Therma ICE 3000 terhadap pengujian logam Pb. Hal ini sangat penting karena berpengaruh besar terhadap validitas hasil pengukuran pada kegiatan riset,praktikum dan pengujian di Laboratorium Kimia UPT Lab Terpadu UNS. Perform a yang akan diteliti meliputi akurasi, presisi, limit deteksi dan liniaritas. Akurasi dilakukan dengan pengukuran CRM 500 dan 697, presisi dilakukan dengan mengukur standar 10 pengulangan, limit deteksi dengan mengukur larutan blangko 10 pengulangan, liniaritas dilakukan dengan mengukur larutan baku dari berbagai deret konsentrasi. Dari pengukuran didapatkan hasil uji linearit...
A study was performed on the antioxidant activity o f the methanol extract of Luffa acutangula (L... more A study was performed on the antioxidant activity o f the methanol extract of Luffa acutangula (L.) Rox b (ridge gourd) fruit (LAM) and its derived fractions, such as n-hexane (LAH), chloroform (LAC), ethyl acetate (LAE), nbutanol (LAB) and residual aqueous fraction (LAA) a nd their correlation to the total phenolics and fla vonoids contents. The phytochemical screening demonstrated the presence of phenolic and flavonoid compounds in all the fractions except the n-hexane fraction. Carotenoids were present in the LAH and LAC, while absent in t he LAE. The antioxidant activity was assessed using β-carotene bleaching method and the results showed t hat LAM, LAH and LAC have significant antioxidant activities in the c omparison with those of BHT and PG. The total pheno lics content of the extract/fractions was determined by the Foli n-C ocalteu procedure and ranged from 18.7±0.11 to 105.1±0.08 mg GAE/g of dried weight basis. The flavonoids cont ent of extract/fractions was measured by a...
Indonesian Journal of Chemistry, 2021
The novel nickel(II) complex has been successfully synthesized through the reaction of Ni(NO 3 ) ... more The novel nickel(II) complex has been successfully synthesized through the reaction of Ni(NO 3 ) 2 ·6H 2 O with 4-aminoantipyrine (AAP) ligand in a 1:3 mole ratio of Ni(II) to AAP. The complex was characterized using UV-Vis, Atomic Absorption Spectroscopy (AAS), Infrared spectrophotometry (IR), Thermogravimetry/Differential Scanning Calorimetry (TG/DSC), conductivity, and magnetic susceptibility. The complex formula was [Ni(AAP) 3 ](NO 3 ) 2 ·5H 2 O. AAP was a bidentate ligand that coordinated through the primary amine nitrogen and the carbonyl oxygen to the nickel ion. The electronic spectra of the complex displayed two peaks at 646 nm and 385 nm in accordance with the 3 A 2g (F) → 3 T 1g (F) and 3 A 2g (F) → 3 T 1g (P) transitions, respectively. This complex gave a paramagnetic property with the effective magnetic moment (µ eff ) of 2.96 BM and the shape of an octahedron. The antibacterial test of this complex showed higher activity than the metal and its free ligand.